Reviewed, UniProtKB/Swiss-Prot P54229 (CTHL5_BOVIN)

Names and origin

Protein names

Recommended name:
    Cathelicidin-5
Alternative name(s):
    Antibacterial peptide BMAP-28
    Myeloid antibacterial peptide 28

Gene names

Name:	CATHL5
Synonyms:	BMAP28

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	159 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Exerts a potent antimicrobial activity against Gram-negative and Gram-positive bacteria, including methicillin-resistant Staphylococcus aureus, and fungi.
Subcellular location	Secreted.
Sequence similarities	Belongs to the cathelicidin family.
Mass spectrometry	Molecular mass is 14414±1 Da from positions 30 - 159. Determined by ESI. Ref.4

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Molecular function	Antibiotic Antimicrobial
PTM	Disulfide bond Pyrrolidone carboxylic acid
Gene Ontology (GO)
Biological process	defense response to bacterium Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 29	29	Potential
	Propeptide	30 – 131	102			PRO_0000004716
	Peptide	132 – 159	28	Cathelicidin-5		PRO_0000004717
Amino acid modifications
	Modified residue	30	1	Pyrrolidone carboxylic acid
	Disulfide bond	86 ↔ 97		By similarity
	Disulfide bond	108 ↔ 125		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P54229-1 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 89B7CB46C5EEC367	FASTA	159	17,616
	10 20 30 40 50 60 METQRASLSL GRWSLWLLLL GLALPSASAQ ALSYREAVLR AVDQLNEKSS EANLYRLLEL 70 80 90 100 110 120 DPPPKEDDEN PNIPKPVSFR VKETVCPRTS QQSPEQCDFK ENGLLKECVG TVTLDQVGSN 130 140 150 FDITCAVPQS VGGLRSLGRK ILRAWKKYGP IIVPIIRIG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Biological characterization of two novel cathelicidin-derived peptides and identification of structural requirements for their antimicrobial and cell lytic activities." Skerlavaj B., Gennaro R., Bagella L., Merluzzi L., Risso A., Zanetti M. J. Biol. Chem. 271:28375-28381(1996) [PubMed: 8910461] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Bone marrow.
[2]	"Sequence analysis and polymorphism discovery in 4 members of the bovine cathelicidin gene family." Gillenwaters E.N., Seabury C.M., Elliott J.S., Womack J.E. J. Hered. 100:241-245(2009) [PubMed: 19136450] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: Isolate 44, Isolate 74 and Isolate JEW38.
[3]	NIH - Mammalian Gene Collection (MGC) project Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Hereford. Tissue: Thymus.
[4]	"Purification and structural characterization of bovine cathelicidins, precursors of antimicrobial peptides." Storici P., Tossi A., Lenarcic B., Romeo D. Eur. J. Biochem. 238:769-776(1996) [PubMed: 8706679] [Abstract] Cited for: CHARACTERIZATION, MASS SPECTROMETRY.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X97609 mRNA. Translation: CAA66208.1. EU751296 Genomic DNA. Translation: ACI31918.1. EU751297 Genomic DNA. Translation: ACI31919.1. EU751302 Genomic DNA. Translation: ACI31924.1. BC142015 mRNA. Translation: AAI42016.1.
IPI	IPI00710530.
RefSeq	NP_776935.1.
UniGene	Bt.15991
3D structure databases
HSSP	HSSP built from PDB template 1KWI based on UniProtKB P32196.
SMR	P54229. Positions 30-131.
ModBase	Search...
Genome annotation databases
Ensembl	ENSBTAG00000020076. Bos taurus. [Contig view]
GeneID	282167.
KEGG	bta:282167.
Phylogenomic databases
HOVERGEN	P54229.
OMA	P54229. QRSSDAN.
Family and domain databases
InterPro	IPR001894. Cathelicidin. IPR018216. Cathelicidin_CS. [Graphical view]
PANTHER	PTHR10206. Cathelicidin. 1 hit.
Pfam	PF00666. Cathelicidins. 1 hit. [Graphical view]
ProDom	PD001838. Cathelicidin. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS00946. CATHELICIDINS_1. 1 hit. PS00947. CATHELICIDINS_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CTHL5_BOVIN

Accession

Primary (citable) accession number: P54229
Secondary accession number(s): A5PJ96, B9UKL6

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	June 16, 2009
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)