P54227 (STMN1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Stathmin

Alternative name(s):
Leukemia-associated gene protein
Leukemia-associated phosphoprotein p18
Metablastin
Oncoprotein 18
Short name=Op18
Phosphoprotein p19
Short name=pp19
Prosolin
Protein Pr22
pp17

Gene names

Name:	Stmn1
Synonyms:	Lag, Lap18, Pr22

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	149 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in the regulation of the microtubule (MT) filament system by destabilizing microtubules. Prevents assembly and promotes disassembly of microtubules. Phosphorylation at Ser-16 may be required for axon formation during neurogenesis By similarity. Involved in the control of the learned and innate fear. Ref.8
Subunit structure	Binds to two alpha/beta-tubulin heterodimers. Interacts with KIST. Ref.7
Subcellular location	Cytoplasm › cytoskeleton.
Tissue specificity	Highly expressed in the lateral nucleus of the amygdala. Ref.8
Post-translational modification	Many different phosphorylated forms are observed depending on specific combinations among the sites which can be phosphorylated. MAPK is responsible for the phosphorylation of stathmin in response to NGF Probable. Phosphorylation at Ser-16 seems to be required for neuron polarization By similarity. Ref.6 Ref.9 Ref.10 Ref.11 Ref.12
Disruption phenotype	Mice show deficits in spike-timing-dependent long-term potentiation, exhibit decreased memory in amygdala-dependent fear conditioning and fail to recognize danger in innately aversive environements. Ref.8
Sequence similarities	Belongs to the stathmin family.

Ontologies

Keywords
Biological process	Differentiation Neurogenesis
Cellular component	Cytoplasm Cytoskeleton Microtubule
Domain	Coiled coil
Molecular function	Developmental protein
PTM	Acetylation Phosphoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	axonogenesis Inferred from mutant phenotype. Source: MGI intracellular signal transduction Inferred from electronic annotation. Source: InterPro negative regulation of microtubule polymerization Inferred from direct assay. Source: MGI positive regulation of cellular component movement Inferred from genetic interaction. Source: MGI
Cellular component	cytosol Inferred from direct assay. Source: MGI membrane fraction Inferred from direct assay. Source: MGI microtubule Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	protein binding Inferred from physical interaction Ref.7. Source: UniProtKB
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
Cdkn1b	P46414	2	EBI-1006438,EBI-1005742

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 149	148	Stathmin		PRO_0000182390
Regions
	Coiled coil	41 – 140	100	Potential
Amino acid modifications
	Modified residue	2	1	N-acetylalanine By similarity
	Modified residue	9	1	N6-acetyllysine By similarity
	Modified residue	16	1	Phosphoserine; by PKA Ref.6 Ref.12
	Modified residue	25	1	Phosphoserine; by CDK1, MAPK1 and MAPK3 Ref.6 Ref.9
	Modified residue	28	1	Phosphoserine By similarity
	Modified residue	38	1	Phosphoserine; by CDK1, MAPK1 and MAPK3 Ref.6 Ref.9 Ref.11
	Modified residue	46	1	Phosphoserine Ref.9
	Modified residue	63	1	Phosphoserine; by PKA Ref.6 Ref.10
	Modified residue	80	1	N6-acetyllysine By similarity
	Modified residue	95	1	N6-acetyllysine By similarity
	Modified residue	100	1	N6-acetyllysine By similarity
	Modified residue	119	1	N6-acetyllysine By similarity
	Modified residue	128	1	N6-acetyllysine By similarity
	Modified residue	146	1	Phosphothreonine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P54227 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: 616526E0A6667BDA	FASTA	149	17,274
	10 20 30 40 50 60 MASSDIQVKE LEKRASGQAF ELILSPRSKE SVPDFPLSPP KKKDLSLEEI QKKLEAAEER 70 80 90 100 110 120 RKSHEAEVLK QLAEKREHEK EVLQKAIEEN NNFSKMAEEK LTHKMEANKE NREAQMAAKL 130 140 ERLREKDKHV EEVRKNKESK DPADETEAD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular diversity of the SCG10/stathmin gene family in the mouse." Okazaki T., Yoshida B.N., Avraham K.B., Wang H., Wuenschell C.W., Jenkins N.A., Copeland N.G., Anderson D.J., Mori N. Genomics 18:360-373(1993) [PubMed: 8288240] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	Erratum Okazaki T., Yoshida B.N., Avraham K.B., Wang H., Wuenschell C.W., Jenkins N.A., Copeland N.G., Anderson D.J., Mori N. Genomics 21:298-298(1994)
[3]	"Transcriptional and post-transcriptional regulation of pr22 (Op18) with proliferation control." Hosoya H., Ishikawa K., Dohi N., Marunouchi T. Cell Struct. Funct. 21:237-243(1996) [PubMed: 8906359] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: C57BL/6.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: 129. Tissue: Mammary gland and Retina.
[5]	Lubec G., Klug S., Yang J.W., Zigmond M. Submitted (JUL-2007) to UniProtKB Cited for: PROTEIN SEQUENCE OF 15-27, MASS SPECTROMETRY. Tissue: Brain and Hippocampus.
[6]	"Multiple phosphorylation of stathmin. Identification of four sites phosphorylated in intact cells and in vitro by cyclic AMP-dependent protein kinase and p34cdc2." Beretta L., Dobransky T., Sobel A. J. Biol. Chem. 268:20076-20084(1993) [PubMed: 8376365] [Abstract] Cited for: PHOSPHORYLATION AT SER-16; SER-25; SER-38 AND SER-63.
[7]	"Stathmin interaction with a putative kinase and coiled-coil-forming protein domains." Maucuer A., Camonis J.H., Sobel A. Proc. Natl. Acad. Sci. U.S.A. 92:3100-3104(1995) [PubMed: 7724523] [Abstract] Cited for: INTERACTION WITH KIST. Tissue: Embryo.
[8]	"Stathmin, a gene enriched in the amygdala, controls both learned and innate fear." Shumyatsky G.P., Malleret G., Shin R.-M., Takizawa S., Tully K., Tsvetkov E., Zakharenko S.S., Joseph J., Vronskaya S., Yin D., Schubart U.K., Kandel E.R., Bolshakov V.Y. Cell 123:697-709(2005) [PubMed: 16286011] [Abstract] Cited for: FUNCTION IN CONTROL OF FEAR, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE.
[9]	"Qualitative and quantitative analyses of protein phosphorylation in naive and stimulated mouse synaptosomal preparations." Munton R.P., Tweedie-Cullen R., Livingstone-Zatchej M., Weinandy F., Waidelich M., Longo D., Gehrig P., Potthast F., Rutishauser D., Gerrits B., Panse C., Schlapbach R., Mansuy I.M. Mol. Cell. Proteomics 6:283-293(2007) [PubMed: 17114649] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-25; SER-38 AND SER-46, MASS SPECTROMETRY. Tissue: Brain cortex.
[10]	"Solid tumor proteome and phosphoproteome analysis by high resolution mass spectrometry." Zanivan S., Gnad F., Wickstroem S.A., Geiger T., Macek B., Cox J., Faessler R., Mann M. J. Proteome Res. 7:5314-5326(2008) [PubMed: 19367708] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-63, MASS SPECTROMETRY. Tissue: Melanoma.
[11]	"The phagosomal proteome in interferon-gamma-activated macrophages." Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P. Immunity 30:143-154(2009) [PubMed: 19144319] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-38, MASS SPECTROMETRY. Tissue: Macrophage.
[12]	"Large scale localization of protein phosphorylation by use of electron capture dissociation mass spectrometry." Sweet S.M., Bailey C.M., Cunningham D.L., Heath J.K., Cooper H.J. Mol. Cell. Proteomics 8:904-912(2009) [PubMed: 19131326] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-16, MASS SPECTROMETRY. Tissue: Embryonic fibroblast.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L20256 Genomic DNA. No translation available. L20257 Genomic DNA. No translation available. L20258 Genomic DNA. No translation available. X94915 mRNA. Translation: CAA64401.1. BC010581 mRNA. Translation: AAH10581.1. BC031831 mRNA. Translation: AAH31831.1. BC054396 mRNA. Translation: AAH54396.1.
IPI	IPI00551236.
PIR	B48917.
RefSeq	NP_062615.1. NM_019641.4.
UniGene	Mm.378957.
3D structure databases
ProteinModelPortal	P54227.
SMR	P54227. Positions 6-141.
ModBase	Search...
Protein-protein interaction databases
IntAct	P54227. 2 interactions.
STRING	P54227.
PTM databases
PhosphoSite	P54227.
2D gel databases
REPRODUCTION-2DPAGE	P54227.
UCD-2DPAGE	P54227.
Proteomic databases
PRIDE	P54227.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000030636; ENSMUSP00000030636; ENSMUSG00000028832. ENSMUST00000105868; ENSMUSP00000101494; ENSMUSG00000028832.
GeneID	16765.
KEGG	mmu:16765.
Organism-specific databases
CTD	3925.
MGI	MGI:96739. Stmn1.
Phylogenomic databases
eggNOG	roNOG12776.
HOGENOM	HBG444413.
HOVERGEN	HBG054037.
InParanoid	P54227.
OMA	KEAVPEF.
OrthoDB	EOG46MBKT.
PhylomeDB	P54227.
Gene expression databases
ArrayExpress	P54227.
Bgee	P54227.
CleanEx	MM_STMN1.
Genevestigator	P54227.
GermOnline	ENSMUSG00000028832. Mus musculus.
Family and domain databases
InterPro	IPR000956. Stathmin. [Graphical view]
KO	K04381.
PANTHER	PTHR10104. Stathmin. 1 hit.
Pfam	PF00836. Stathmin. 1 hit. [Graphical view]
PIRSF	PIRSF002285. Stathmin. 1 hit.
PRINTS	PR00345. STATHMIN.
SUPFAM	SSF101494. Stathmin. 1 hit.
PROSITE	PS00563. STATHMIN_1. 1 hit. PS01041. STATHMIN_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	290586.
SOURCE	Search...

Entry information

Entry name

STMN1_MOUSE

Accession

Primary (citable) accession number: P54227

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	January 23, 2007
Last modified:	January 25, 2012
This is version 106 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program