P54225 (HEMH_SYNY3) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ferrochelatase
EC=4.99.1.1

Alternative name(s):
Heme synthase
Protoheme ferro-lyase

Gene names

Name:	hemH
Ordered Locus Names:	slr0839

Organism

Synechocystis sp. (strain PCC 6803 / Kazusa)

Taxonomic identifier

1111708 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Chroococcales › Synechocystis

Protein attributes

Sequence length	387 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the ferrous insertion into protoporphyrin IX. HAMAP MF_00323
Catalytic activity	Protoheme + 2 H+ = protoporphyrin + Fe2+. HAMAP MF_00323
Pathway	Porphyrin metabolism; protoheme biosynthesis; protoheme from protoporphyrin-IX: step 1/1. HAMAP MF_00323
Subcellular location	Cytoplasm By similarity HAMAP MF_00323.
Sequence similarities	Belongs to the ferrochelatase family.

Ontologies

Keywords
Biological process	Heme biosynthesis Porphyrin biosynthesis
Cellular component	Cytoplasm
Ligand	Iron Metal-binding
Molecular function	Lyase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	heme biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ferrochelatase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 387	387	Ferrochelatase HAMAP MF_00323		PRO_0000175218
Sites
	Metal binding	196	1	Iron By similarity
	Metal binding	277	1	Iron By similarity

Sequences

	Sequence		Length	Mass (Da)
	P54225 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 29FF8675168FF308	FASTA	387	43,924
	10 20 30 40 50 60 MGRVGVLLLN LGGPEKLEDV RPFLFNLFAD PEIIRLPFPW LQKPLAWLIS TLRAKKSQAN 70 80 90 100 110 120 YAEIGGGSPL LQITEAQASA LTTRLERLGQ DAKVYIGMRY WHPFTEEAVE KIKGDRLQRL 130 140 150 160 170 180 VILPLYPHFS ISTSGSSFRV LEEMWHNDPS LRQLDYSLIP SWYDHPGYLQ AMADLIAQEL 190 200 210 220 230 240 KKFPNPDQAH IFFSAHGVPQ SYVDEAGDPY QAEIEACTRL IMRTLDRPNQ YTLAYQSRVG 250 260 270 280 290 300 PVEWLKPYTE EALQKLGAEG IDDLLVVPIS FVSEHIETLQ EIDIEYREIA EEAGIDNFQR 310 320 330 340 350 360 VPALNTHPVF IDALAQMVMD SLNDPPCTFE TVPHPKKNMK MYPQERWEWG LTTAAEVWNG 370 380 RLAMLGFIAL LVELISGQGP LHFVGLL

References

[1]	"Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. I. Sequence features in the 1 Mb region from map positions 64% to 92% of the genome." Kaneko T., Tanaka A., Sato S., Kotani H., Sazuka T., Miyajima N., Sugiura M., Tabata S. DNA Res. 2:153-166(1995) [PubMed: 8590279] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 27184 / PCC 6803 / N-1.
[2]	"Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions." Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y., Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T., Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S. , Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S. DNA Res. 3:109-136(1996) [PubMed: 8905231] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 27184 / PCC 6803 / N-1.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000022 Genomic DNA. Translation: BAA10523.1.
PIR	S75788.
RefSeq	NP_442453.1. NC_000911.1.
3D structure databases
ProteinModelPortal	P54225.
ModBase	Search...
Protein-protein interaction databases
IntAct	P54225. 5 interactions.
STRING	P54225.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	954446.
GenomeReviews	Gene locus slr0839 in contig BA000022_GR.
KEGG	syn:slr0839.
NMPDR	fig|1148.1.peg.2554.
PATRIC	23842608. VBISynSp132158_2843.
Phylogenomic databases
eggNOG	COG0276.
HOGENOM	HBG697135.
OMA	LQKPLAW.
PhylomeDB	P54225.
ProtClustDB	PRK00035.
Enzyme and pathway databases
BioCyc	SSP1148:SLR0839-MONOMER.
BRENDA	4.99.1.1. 6185.
Family and domain databases
HAMAP	MF_00323. Ferrochelatase. [Tree]
InterPro	IPR023329. Chlorophyll_a/b-bd_dom. IPR001015. Ferrochelatase. IPR019772. Ferrochelatase_AS. [Graphical view]
Gene3D	G3DSA:1.10.3460.10. G3DSA:1.10.3460.10. 1 hit.
KO	K01772.
PANTHER	PTHR11108. Ferrochelatase. 1 hit.
Pfam	PF00762. Ferrochelatase. 1 hit. [Graphical view]
SUPFAM	SSF103511. SSF103511. 1 hit.
TIGRFAMs	TIGR00109. HemH. 1 hit.
PROSITE	PS00534. FERROCHELATASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HEMH_SYNY3

Accession

Primary (citable) accession number: P54225

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	January 25, 2012
This is version 77 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program