P54223 (BETA_RHIME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Choline dehydrogenase
Short name=CDH
Short name=CHD
EC=1.1.99.1

Gene names

Name:	betA
Ordered Locus Names:	R00947
ORF Names:	SMc00093

Organism

Rhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium meliloti) [Complete proteome] [HAMAP]

Taxonomic identifier

266834 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Sinorhizobium/Ensifer group › Sinorhizobium

Protein attributes

Sequence length	549 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Can catalyze the oxidation of choline to betaine aldehyde and betaine aldehyde to glycine betaine By similarity. HAMAP MF_00750
Catalytic activity	Choline + acceptor = betaine aldehyde + reduced acceptor. HAMAP MF_00750
Cofactor	FAD.
Pathway	Amine and polyamine biosynthesis; betaine biosynthesis via choline pathway; betaine aldehyde from choline (cytochrome c reductase route): step 1/1. HAMAP MF_00750
Subcellular location	Cell membrane; Peripheral membrane protein HAMAP MF_00750.
Sequence similarities	Belongs to the GMC oxidoreductase family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Ligand	FAD Flavoprotein
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycine betaine biosynthetic process from choline Inferred from electronic annotation. Source: InterPro
Cellular component	plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	choline dehydrogenase activity Inferred from electronic annotation. Source: EC flavin adenine dinucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 549	549	Choline dehydrogenase HAMAP MF_00750		PRO_0000205594
Regions
	Nucleotide binding	4 – 33	30	FAD Potential
Sites
	Active site	465	1	By similarity
Experimental info
	Sequence conflict	267	1	A → R in AAC13369. Ref.1
	Sequence conflict	417 – 424	8	RHCVRLTR → DLRAVTG in AAC13369. Ref.1
	Sequence conflict	429	1	Q → E in AAC13369. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P54223 [UniParc]. Last modified May 30, 2000. Version 2. Checksum: 29BE064F40CB88F4	FASTA	549	61,291
	10 20 30 40 50 60 MQADFVIIGS GSAGSALAYR LSEDGRNSVI VLEFGGSDVG PFIQMPAALA WPMSMNRYNW 70 80 90 100 110 120 GYLSEPEPNL NNRRITAPRG KVIGGSSSIN GMVYVRGHSE DFNRWEELGA QGWAYADVLP 130 140 150 160 170 180 YYKRMEHSHG GEEGWRGTDG PLHVQRGPVK NPLFHAFIEA GKEAGFEVTE DYNGSKQEGF 190 200 210 220 230 240 GLMEQTTWRG RRWSAASAYL RPALKRPNVE LIRCFARKIV IENGRATGVE IERGGRIEVV 250 260 270 280 290 300 KANREVIVSA SSFNSPKLLM LSGIGPAAHL KEMGIDVKVD RPGVGQNLQD HMEFYFQQVS 310 320 330 340 350 360 TKPVSLYSWL PWFWQGVAGA QWLFFKRGLG ISNQFESCAF LRSAPGVKQP DIQYHFLPVA 370 380 390 400 410 420 ISYDGKAAAK SHGFQVHVGY NLSKSRGNVS LRSSDPKADP VIRFNYMSHP EDWEKFRHCV 430 440 450 460 470 480 RLTREIFGQK AFDLYRGPEI QPGEKVQTDE EIDGFLREHL ESAYHPCGTC KMGAKDDPMA 490 500 510 520 530 540 VVDPETRVIG VDGLRVADSS IFPHITYGNL NAPSIMTGEK SADHILGRQP LARSNQEPWI NPRWAVSDR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular characterization of the bet genes encoding glycine betaine synthesis in Sinorhizobium meliloti 102F34." Pocard J.A., Vincent N., Boncompagni E., Tombras Smith L., Poggi M.-C., Le Rudulier D. Microbiology 143:1369-1379(1997) [PubMed: 9141699] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 102F34.
[2]	"Analysis of the chromosome sequence of the legume symbiont Sinorhizobium meliloti strain 1021." Capela D., Barloy-Hubler F., Gouzy J., Bothe G., Ampe F., Batut J., Boistard P., Becker A., Boutry M., Cadieu E., Dreano S., Gloux S., Godrie T., Goffeau A., Kahn D., Kiss E., Lelaure V., Masuy D. , Pohl T., Portetelle D., Puehler A., Purnelle B., Ramsperger U., Renard C., Thebault P., Vandenbol M., Weidner S., Galibert F. Proc. Natl. Acad. Sci. U.S.A. 98:9877-9882(2001) [PubMed: 11481430] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1021.
[3]	"The composite genome of the legume symbiont Sinorhizobium meliloti." Galibert F., Finan T.M., Long S.R., Puehler A., Abola P., Ampe F., Barloy-Hubler F., Barnett M.J., Becker A., Boistard P., Bothe G., Boutry M., Bowser L., Buhrmester J., Cadieu E., Capela D., Chain P., Cowie A. , Davis R.W., Dreano S., Federspiel N.A., Fisher R.F., Gloux S., Godrie T., Goffeau A., Golding B., Gouzy J., Gurjal M., Hernandez-Lucas I., Hong A., Huizar L., Hyman R.W., Jones T., Kahn D., Kahn M.L., Kalman S., Keating D.H., Kiss E., Komp C., Lelaure V., Masuy D., Palm C., Peck M.C., Pohl T.M., Portetelle D., Purnelle B., Ramsperger U., Surzycki R., Thebault P., Vandenbol M., Vorhoelter F.J., Weidner S., Wells D.H., Wong K., Yeh K.-C., Batut J. Science 293:668-672(2001) [PubMed: 11474104] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1021.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U39940 Genomic DNA. Translation: AAC13369.1. AL591688 Genomic DNA. Translation: CAC45519.1.
RefSeq	NP_385053.1. NC_003047.1.
3D structure databases
ProteinModelPortal	P54223.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1232590.
GenomeReviews	Gene locus R00947 in contig AL591688_GR.
KEGG	sme:SMc00093.
NMPDR	fig|266834.1.peg.2241.
PATRIC	23631169. VBISinMel96828_2346.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG734713.
OMA	GPFIQMP.
ProtClustDB	PRK02106.
Enzyme and pathway databases
BioCyc	MetaCyc:MONOMER-244. SMEL266834:SMC00093-MONOMER.
Family and domain databases
HAMAP	MF_00750. Choline_dehydrogen. [Tree]
InterPro	IPR011533. Choline_dehydrogenase. IPR012132. GMC_OxRdtase. IPR000172. GMC_OxRdtase_N. IPR007867. GMC_OxRtase_C. [Graphical view]
KO	K00108.
Pfam	PF05199. GMC_oxred_C. 1 hit. PF00732. GMC_oxred_N. 1 hit. [Graphical view]
PIRSF	PIRSF000137. Alcohol_oxidase. 1 hit.
TIGRFAMs	TIGR01810. BetA. 1 hit.
PROSITE	PS00623. GMC_OXRED_1. 1 hit. PS00624. GMC_OXRED_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BETA_RHIME

Accession

Primary (citable) accession number: P54223

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	May 30, 2000
Last modified:	January 25, 2012
This is version 88 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program