Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P54223 (BETA_RHIME) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 102. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Oxygen-dependent choline dehydrogenase

Short name=CDH
Short name=CHD
EC=1.1.99.1
Alternative name(s):
Betaine aldehyde dehydrogenase
Short name=BADH
EC=1.2.1.8
Gene names
Name:betA
Ordered Locus Names:R00947
ORF Names:SMc00093
OrganismRhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium meliloti) [Complete proteome] [HAMAP]
Taxonomic identifier266834 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeSinorhizobium/Ensifer groupSinorhizobium

Protein attributes

Sequence length549 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Involved in the biosynthesis of the osmoprotectant glycine betaine. Catalyzes the oxidation of choline to betaine aldehyde and betaine aldehyde to glycine betaine at the same rate. Ref.1

Catalytic activity

Choline + acceptor = betaine aldehyde + reduced acceptor. HAMAP-Rule MF_00750

Betaine aldehyde + NAD+ + H2O = betaine + NADH. HAMAP-Rule MF_00750

Cofactor

FAD By similarity. HAMAP-Rule MF_00750

Pathway

Amine and polyamine biosynthesis; betaine biosynthesis via choline pathway; betaine aldehyde from choline (cytochrome c reductase route): step 1/1. HAMAP-Rule MF_00750

Subcellular location

Cell membrane; Peripheral membrane protein Ref.1.

Sequence similarities

Belongs to the GMC oxidoreductase family.

Ontologies

Keywords
   Cellular componentCell membrane
Membrane
   LigandFAD
Flavoprotein
NAD
   Molecular functionOxidoreductase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processglycine betaine biosynthetic process from choline

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentplasma membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionbetaine-aldehyde dehydrogenase activity

Inferred from electronic annotation. Source: UniProtKB-EC

choline dehydrogenase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

flavin adenine dinucleotide binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 549549Oxygen-dependent choline dehydrogenase HAMAP-Rule MF_00750
PRO_0000205594

Regions

Nucleotide binding4 – 3330FAD By similarity

Sites

Active site4651 By similarity

Experimental info

Sequence conflict2671A → R in AAC13369. Ref.1
Sequence conflict417 – 4248RHCVRLTR → DLRAVTG in AAC13369. Ref.1
Sequence conflict4291Q → E in AAC13369. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P54223 [UniParc].

Last modified May 30, 2000. Version 2.
Checksum: 29BE064F40CB88F4

FASTA54961,291
        10         20         30         40         50         60 
MQADFVIIGS GSAGSALAYR LSEDGRNSVI VLEFGGSDVG PFIQMPAALA WPMSMNRYNW 

        70         80         90        100        110        120 
GYLSEPEPNL NNRRITAPRG KVIGGSSSIN GMVYVRGHSE DFNRWEELGA QGWAYADVLP 

       130        140        150        160        170        180 
YYKRMEHSHG GEEGWRGTDG PLHVQRGPVK NPLFHAFIEA GKEAGFEVTE DYNGSKQEGF 

       190        200        210        220        230        240 
GLMEQTTWRG RRWSAASAYL RPALKRPNVE LIRCFARKIV IENGRATGVE IERGGRIEVV 

       250        260        270        280        290        300 
KANREVIVSA SSFNSPKLLM LSGIGPAAHL KEMGIDVKVD RPGVGQNLQD HMEFYFQQVS 

       310        320        330        340        350        360 
TKPVSLYSWL PWFWQGVAGA QWLFFKRGLG ISNQFESCAF LRSAPGVKQP DIQYHFLPVA 

       370        380        390        400        410        420 
ISYDGKAAAK SHGFQVHVGY NLSKSRGNVS LRSSDPKADP VIRFNYMSHP EDWEKFRHCV 

       430        440        450        460        470        480 
RLTREIFGQK AFDLYRGPEI QPGEKVQTDE EIDGFLREHL ESAYHPCGTC KMGAKDDPMA 

       490        500        510        520        530        540 
VVDPETRVIG VDGLRVADSS IFPHITYGNL NAPSIMTGEK SADHILGRQP LARSNQEPWI 


NPRWAVSDR 

« Hide

References

« Hide 'large scale' references
[1]"Molecular characterization of the bet genes encoding glycine betaine synthesis in Sinorhizobium meliloti 102F34."
Pocard J.A., Vincent N., Boncompagni E., Tombras Smith L., Poggi M.-C., Le Rudulier D.
Microbiology 143:1369-1379(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBCELLULAR LOCATION.
Strain: 102F34.
[2]"Analysis of the chromosome sequence of the legume symbiont Sinorhizobium meliloti strain 1021."
Capela D., Barloy-Hubler F., Gouzy J., Bothe G., Ampe F., Batut J., Boistard P., Becker A., Boutry M., Cadieu E., Dreano S., Gloux S., Godrie T., Goffeau A., Kahn D., Kiss E., Lelaure V., Masuy D. expand/collapse author list , Pohl T., Portetelle D., Puehler A., Purnelle B., Ramsperger U., Renard C., Thebault P., Vandenbol M., Weidner S., Galibert F.
Proc. Natl. Acad. Sci. U.S.A. 98:9877-9882(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 1021.
[3]"The composite genome of the legume symbiont Sinorhizobium meliloti."
Galibert F., Finan T.M., Long S.R., Puehler A., Abola P., Ampe F., Barloy-Hubler F., Barnett M.J., Becker A., Boistard P., Bothe G., Boutry M., Bowser L., Buhrmester J., Cadieu E., Capela D., Chain P., Cowie A. expand/collapse author list , Davis R.W., Dreano S., Federspiel N.A., Fisher R.F., Gloux S., Godrie T., Goffeau A., Golding B., Gouzy J., Gurjal M., Hernandez-Lucas I., Hong A., Huizar L., Hyman R.W., Jones T., Kahn D., Kahn M.L., Kalman S., Keating D.H., Kiss E., Komp C., Lelaure V., Masuy D., Palm C., Peck M.C., Pohl T.M., Portetelle D., Purnelle B., Ramsperger U., Surzycki R., Thebault P., Vandenbol M., Vorhoelter F.J., Weidner S., Wells D.H., Wong K., Yeh K.-C., Batut J.
Science 293:668-672(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 1021.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U39940 Genomic DNA. Translation: AAC13369.1.
AL591688 Genomic DNA. Translation: CAC45519.1.
RefSeqNP_385053.1. NC_003047.1.

3D structure databases

ProteinModelPortalP54223.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING266834.SMc00093.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAC45519; CAC45519; SMc00093.
GeneID1232590.
KEGGsme:SMc00093.
PATRIC23631169. VBISinMel96828_2346.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG2303.
HOGENOMHOG000139600.
KOK00108.
OMAREMHCPR.
OrthoDBEOG67HJQP.

Enzyme and pathway databases

BioCycMetaCyc:MONOMER-244.
SMEL266834:GJF6-970-MONOMER.
UniPathwayUPA00529; UER00385.

Family and domain databases

HAMAPMF_00750. Choline_dehydrogen.
InterProIPR011533. Choline_dehydrogenase.
IPR012132. GMC_OxRdtase.
IPR000172. GMC_OxRdtase_N.
IPR007867. GMC_OxRtase_C.
[Graphical view]
PfamPF05199. GMC_oxred_C. 1 hit.
PF00732. GMC_oxred_N. 1 hit.
[Graphical view]
PIRSFPIRSF000137. Alcohol_oxidase. 1 hit.
TIGRFAMsTIGR01810. betA. 1 hit.
PROSITEPS00623. GMC_OXRED_1. 1 hit.
PS00624. GMC_OXRED_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameBETA_RHIME
AccessionPrimary (citable) accession number: P54223
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: May 30, 2000
Last modified: May 14, 2014
This is version 102 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways