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P54222 (BETB1_RHIME) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 91. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
NAD/NADP-dependent betaine aldehyde dehydrogenase 1

Short name=BADH 1
EC=1.2.1.8
Gene names
Name:betB1
Synonyms:betB
Ordered Locus Names:R00948
ORF Names:SMc00094
OrganismRhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium meliloti) [Complete proteome] [HAMAP]
Taxonomic identifier266834 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeSinorhizobium/Ensifer groupSinorhizobium

Protein attributes

Sequence length487 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Involved in the biosynthesis of the osmoprotectant glycine betaine. Catalyzes the reversible oxidation of betaine aldehyde to the corresponding acid By similarity. HAMAP-Rule MF_00804

Catalytic activity

Betaine aldehyde + NAD+ + H2O = betaine + NADH. HAMAP-Rule MF_00804

Cofactor

Binds 2 potassium ions per subunit By similarity. HAMAP-Rule MF_00804

Pathway

Amine and polyamine biosynthesis; betaine biosynthesis via choline pathway; betaine from betaine aldehyde: step 1/1. HAMAP-Rule MF_00804

Subunit structure

Dimer of dimers By similarity. HAMAP-Rule MF_00804

Sequence similarities

Belongs to the aldehyde dehydrogenase family.

Ontologies

Keywords
   LigandMetal-binding
NAD
NADP
Potassium
   Molecular functionOxidoreductase
   PTMOxidation
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processglycine betaine biosynthetic process from choline

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Molecular_functionbetaine-aldehyde dehydrogenase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 487487NAD/NADP-dependent betaine aldehyde dehydrogenase 1 HAMAP-Rule MF_00804
PRO_0000056553

Regions

Nucleotide binding150 – 1534NAD/NADP By similarity
Nucleotide binding176 – 1794NAD/NADP By similarity
Nucleotide binding228 – 2336NAD/NADP By similarity

Sites

Active site1621Charge relay system By similarity
Active site2501Proton acceptor By similarity
Active site4611Charge relay system By similarity
Metal binding261Potassium 1 By similarity
Metal binding271Potassium 1; via carbonyl oxygen By similarity
Metal binding931Potassium 1 By similarity
Metal binding2441Potassium 2; via carbonyl oxygen By similarity
Metal binding4541Potassium 2; via carbonyl oxygen By similarity
Metal binding4571Potassium 2; via carbonyl oxygen By similarity
Binding site2841NAD/NADP By similarity
Binding site3841NAD/NADP By similarity

Amino acid modifications

Modified residue2841Cysteine sulfenic acid (-SOH) By similarity

Experimental info

Sequence conflict821R → L in AAC13368. Ref.1
Sequence conflict3741V → D in AAC13368. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P54222 [UniParc].

Last modified May 30, 2000. Version 2.
Checksum: D548CDF0BB1329AA

FASTA48751,549
        10         20         30         40         50         60 
MRAQPKASHF IDGEYVEDAA GTVIESIYPA TGEIIARLHA ATPGIVEKAI AAAKRAQPEW 

        70         80         90        100        110        120 
AAMSPTARGR ILKRAAELMR QRNRELSELE TLDTGKPIQE TIVADPTSGA DSFEFFGGVA 

       130        140        150        160        170        180 
PAALNGDYIP LGGDFAYTKR VPLGVCVGIG AWNYPQQIAC WKGAPALVAG NAMVFKPSEN 

       190        200        210        220        230        240 
TPLGALKIAE ILIEAGLPKG LFNVIQGDRA TGPLLVNHPD VAKVSLTGSV PTGKKVAGAA 

       250        260        270        280        290        300 
AAELKHVTME LGGKSPLIVF DDADLESAIG GAMLGNFYST GQVCSNGTRV FVQRKIKEPF 

       310        320        330        340        350        360 
LARLKERTEA IVIGDPLDEA TQLGPMVSAA QRDKVFSYIG KGKAEGARLV TGGGIPNNVS 

       370        380        390        400        410        420 
GEGTYIQPTV FADVTDGMTI AREEIFGPVM CVLDFDDEVE VIARANATEF GLSAGVFTAD 

       430        440        450        460        470        480 
LTRAHRVADR LEAGTLWINT YNLCPVEIPF GGSKQSGFGR ENSVAALNHY TELKTVYVGM 


GPVEAPY 

« Hide

References

« Hide 'large scale' references
[1]"Molecular characterization of the bet genes encoding glycine betaine synthesis in Sinorhizobium meliloti 102F34."
Pocard J.A., Vincent N., Boncompagni E., Tombras Smith L., Poggi M.-C., Le Rudulier D.
Microbiology 143:1369-1379(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 102F34.
[2]"Analysis of the chromosome sequence of the legume symbiont Sinorhizobium meliloti strain 1021."
Capela D., Barloy-Hubler F., Gouzy J., Bothe G., Ampe F., Batut J., Boistard P., Becker A., Boutry M., Cadieu E., Dreano S., Gloux S., Godrie T., Goffeau A., Kahn D., Kiss E., Lelaure V., Masuy D. expand/collapse author list , Pohl T., Portetelle D., Puehler A., Purnelle B., Ramsperger U., Renard C., Thebault P., Vandenbol M., Weidner S., Galibert F.
Proc. Natl. Acad. Sci. U.S.A. 98:9877-9882(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 1021.
[3]"The composite genome of the legume symbiont Sinorhizobium meliloti."
Galibert F., Finan T.M., Long S.R., Puehler A., Abola P., Ampe F., Barloy-Hubler F., Barnett M.J., Becker A., Boistard P., Bothe G., Boutry M., Bowser L., Buhrmester J., Cadieu E., Capela D., Chain P., Cowie A. expand/collapse author list , Davis R.W., Dreano S., Federspiel N.A., Fisher R.F., Gloux S., Godrie T., Goffeau A., Golding B., Gouzy J., Gurjal M., Hernandez-Lucas I., Hong A., Huizar L., Hyman R.W., Jones T., Kahn D., Kahn M.L., Kalman S., Keating D.H., Kiss E., Komp C., Lelaure V., Masuy D., Palm C., Peck M.C., Pohl T.M., Portetelle D., Purnelle B., Ramsperger U., Surzycki R., Thebault P., Vandenbol M., Vorhoelter F.J., Weidner S., Wells D.H., Wong K., Yeh K.-C., Batut J.
Science 293:668-672(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 1021.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U39940 Genomic DNA. Translation: AAC13368.1.
AL591688 Genomic DNA. Translation: CAC45520.1.
RefSeqNP_385054.1. NC_003047.1.

3D structure databases

ProteinModelPortalP54222.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING266834.SMc00094.

Proteomic databases

ProMEXP54222.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAC45520; CAC45520; SMc00094.
GeneID1232591.
KEGGsme:SMc00094.
PATRIC23631171. VBISinMel96828_2347.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1012.
HOGENOMHOG000271505.
KOK00130.
OMAFEARVDI.
OrthoDBEOG6BS8QW.
ProtClustDBPRK13252.

Enzyme and pathway databases

BioCycMetaCyc:MONOMER-246.
SMEL266834:GJF6-971-MONOMER.
UniPathwayUPA00529; UER00386.

Family and domain databases

Gene3D3.40.309.10. 1 hit.
3.40.605.10. 1 hit.
HAMAPMF_00804. BADH.
InterProIPR016161. Ald_DH/histidinol_DH.
IPR016163. Ald_DH_C.
IPR016160. Ald_DH_CS.
IPR016162. Ald_DH_N.
IPR015590. Aldehyde_DH_dom.
IPR011264. BADH.
[Graphical view]
PfamPF00171. Aldedh. 1 hit.
[Graphical view]
SUPFAMSSF53720. SSF53720. 1 hit.
TIGRFAMsTIGR01804. BADH. 1 hit.
PROSITEPS00070. ALDEHYDE_DEHYDR_CYS. 1 hit.
PS00687. ALDEHYDE_DEHYDR_GLU. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameBETB1_RHIME
AccessionPrimary (citable) accession number: P54222
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: May 30, 2000
Last modified: February 19, 2014
This is version 91 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways