Reviewed, UniProtKB/Swiss-Prot P54222 (BETB1_RHIME)

Names and origin

Protein names

Recommended name:
    Betaine aldehyde dehydrogenase 1
      Short name=BADH 1
    EC=1.2.1.8

Gene names

Name:	betB1
Synonyms:	betB
Ordered Locus Names:	R00948
ORF Names:	SMc00094

Organism

Rhizobium meliloti (Sinorhizobium meliloti) [Complete proteome] [HAMAP]

Taxonomic identifier

382 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Sinorhizobium/Ensifer group › Sinorhizobium

Protein attributes

Sequence length	487 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Betaine aldehyde + NAD+ + H2O = betaine + NADH. HAMAP MF_00804
Pathway	Amine and polyamine biosynthesis; betaine biosynthesis via choline pathway; betaine from betaine aldehyde: step 1/1. HAMAP MF_00804
Sequence similarities	Belongs to the aldehyde dehydrogenase family.

Ontologies

Keywords
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycine betaine biosynthetic process from choline Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	betaine-aldehyde dehydrogenase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 487	487	Betaine aldehyde dehydrogenase 1 HAMAP MF_00804		PRO_0000056553
Regions
	Nucleotide binding	228 – 233	6	NAD By similarity
Sites
	Active site	250	1	By similarity
	Active site	284	1	By similarity
Experimental info
	Sequence conflict	82	1	R → L in AAC13368. Ref.1
	Sequence conflict	374	1	V → D in AAC13368. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P54222-1 [UniParc]. Last modified May 30, 2000. Version 2. Checksum: D548CDF0BB1329AA	FASTA	487	51,549
	10 20 30 40 50 60 MRAQPKASHF IDGEYVEDAA GTVIESIYPA TGEIIARLHA ATPGIVEKAI AAAKRAQPEW 70 80 90 100 110 120 AAMSPTARGR ILKRAAELMR QRNRELSELE TLDTGKPIQE TIVADPTSGA DSFEFFGGVA 130 140 150 160 170 180 PAALNGDYIP LGGDFAYTKR VPLGVCVGIG AWNYPQQIAC WKGAPALVAG NAMVFKPSEN 190 200 210 220 230 240 TPLGALKIAE ILIEAGLPKG LFNVIQGDRA TGPLLVNHPD VAKVSLTGSV PTGKKVAGAA 250 260 270 280 290 300 AAELKHVTME LGGKSPLIVF DDADLESAIG GAMLGNFYST GQVCSNGTRV FVQRKIKEPF 310 320 330 340 350 360 LARLKERTEA IVIGDPLDEA TQLGPMVSAA QRDKVFSYIG KGKAEGARLV TGGGIPNNVS 370 380 390 400 410 420 GEGTYIQPTV FADVTDGMTI AREEIFGPVM CVLDFDDEVE VIARANATEF GLSAGVFTAD 430 440 450 460 470 480 LTRAHRVADR LEAGTLWINT YNLCPVEIPF GGSKQSGFGR ENSVAALNHY TELKTVYVGM GPVEAPY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular characterization of the bet genes encoding glycine betaine synthesis in Sinorhizobium meliloti 102F34." Pocard J.A., Vincent N., Boncompagni E., Tombras Smith L., Poggi M.-C., Le Rudulier D. Microbiology 143:1369-1379(1997) [PubMed: 9141699] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 102F34.
[2]	"Analysis of the chromosome sequence of the legume symbiont Sinorhizobium meliloti strain 1021." Capela D., Barloy-Hubler F., Gouzy J., Bothe G., Ampe F., Batut J., Boistard P., Becker A., Boutry M., Cadieu E., Dreano S., Gloux S., Godrie T., Goffeau A., Kahn D., Kiss E., Lelaure V., Masuy D. , Pohl T., Portetelle D., Puehler A., Purnelle B., Ramsperger U., Renard C., Thebault P., Vandenbol M., Weidner S., Galibert F. Proc. Natl. Acad. Sci. U.S.A. 98:9877-9882(2001) [PubMed: 11481430] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1021.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U39940 Genomic DNA. Translation: AAC13368.1. AL591688 Genomic DNA. Translation: CAC45520.1.
RefSeq	NP_385054.1.
3D structure databases
HSSP	HSSP built from PDB template 1A4S based on UniProtKB P56533.
ModBase	Search...
Genome annotation databases
GeneID	1232591.
GenomeReviews	Gene locus R00948 in contig AL591688_GR.
KEGG	sme:SMc00094.
NMPDR	fig|266834.1.peg.2242.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P54222.
OMA	VFKPSEN.
Enzyme and pathway databases
BioCyc	MetaCyc:MON-246. SMEL266834:SMC00094-MON.
BRENDA	1.2.1.8. 142.
Family and domain databases
HAMAP	MF_00804. [Tree]
InterPro	IPR016160. Ald_DH_CS. IPR016162. Ald_DH_N. IPR015590. Aldehyde_DH. IPR011264. Betaine_Ald_DH. [Graphical view]
Gene3D	G3DSA:3.40.605.10. Aldehyde_dehydrogenase_N. 1 hit.
PANTHER	PTHR11699. Aldehyde_dehyd. 1 hit.
Pfam	PF00171. Aldedh. 1 hit. [Graphical view]
TIGRFAMs	TIGR01804. BADH. 1 hit.
PROSITE	PS00070. ALDEHYDE_DEHYDR_CYS. 1 hit. PS00687. ALDEHYDE_DEHYDR_GLU. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BETB1_RHIME

Accession

Primary (citable) accession number: P54222

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	May 30, 2000
Last modified:	November 3, 2009
This is version 60 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)