Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

NAD/NADP-dependent betaine aldehyde dehydrogenase 1

Gene

betB1

Organism
Rhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium meliloti)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in the biosynthesis of the osmoprotectant glycine betaine. Catalyzes the reversible oxidation of betaine aldehyde to the corresponding acid.UniRule annotation

Catalytic activityi

Betaine aldehyde + NAD+ + H2O = betaine + NADH.UniRule annotation

Cofactori

K(+)UniRule annotationNote: Binds 2 potassium ions per subunit.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi26 – 261Potassium 1UniRule annotation
Metal bindingi27 – 271Potassium 1; via carbonyl oxygenUniRule annotation
Metal bindingi93 – 931Potassium 1UniRule annotation
Active sitei162 – 1621Charge relay systemUniRule annotation
Metal bindingi244 – 2441Potassium 2; via carbonyl oxygenUniRule annotation
Active sitei250 – 2501Proton acceptorUniRule annotation
Binding sitei284 – 2841NAD/NADPUniRule annotation
Binding sitei384 – 3841NAD/NADPUniRule annotation
Metal bindingi454 – 4541Potassium 2; via carbonyl oxygenUniRule annotation
Metal bindingi457 – 4571Potassium 2; via carbonyl oxygenUniRule annotation
Active sitei461 – 4611Charge relay systemUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi150 – 1534NAD/NADPUniRule annotation
Nucleotide bindingi176 – 1794NAD/NADPUniRule annotation
Nucleotide bindingi228 – 2336NAD/NADPUniRule annotation

GO - Molecular functioni

  1. betaine-aldehyde dehydrogenase activity Source: UniProtKB-HAMAP
  2. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. glycine betaine biosynthetic process from choline Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

Metal-binding, NAD, NADP, Potassium

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-246.
SMEL266834:GJF6-971-MONOMER.
UniPathwayiUPA00529; UER00386.

Names & Taxonomyi

Protein namesi
Recommended name:
NAD/NADP-dependent betaine aldehyde dehydrogenase 1UniRule annotation (EC:1.2.1.8UniRule annotation)
Short name:
BADH 1UniRule annotation
Gene namesi
Name:betB1UniRule annotation
Synonyms:betB
Ordered Locus Names:R00948
ORF Names:SMc00094
OrganismiRhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium meliloti)
Taxonomic identifieri266834 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeSinorhizobium/Ensifer groupSinorhizobium
ProteomesiUP000001976: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 487487NAD/NADP-dependent betaine aldehyde dehydrogenase 1PRO_0000056553Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei284 – 2841Cysteine sulfenic acid (-SOH)UniRule annotation

Keywords - PTMi

Oxidation

Interactioni

Subunit structurei

Dimer of dimers.UniRule annotation

Protein-protein interaction databases

STRINGi266834.SMc00094.

Structurei

3D structure databases

ProteinModelPortaliP54222.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the aldehyde dehydrogenase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG1012.
HOGENOMiHOG000271505.
KOiK00130.
OMAiHVYQNIA.
OrthoDBiEOG6BS8QW.

Family and domain databases

Gene3Di3.40.309.10. 1 hit.
3.40.605.10. 1 hit.
HAMAPiMF_00804. BADH.
InterProiIPR016161. Ald_DH/histidinol_DH.
IPR016163. Ald_DH_C.
IPR016160. Ald_DH_CS_CYS.
IPR029510. Ald_DH_CS_GLU.
IPR016162. Ald_DH_N.
IPR015590. Aldehyde_DH_dom.
IPR011264. BADH.
[Graphical view]
PfamiPF00171. Aldedh. 1 hit.
[Graphical view]
SUPFAMiSSF53720. SSF53720. 1 hit.
TIGRFAMsiTIGR01804. BADH. 1 hit.
PROSITEiPS00070. ALDEHYDE_DEHYDR_CYS. 1 hit.
PS00687. ALDEHYDE_DEHYDR_GLU. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P54222-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MRAQPKASHF IDGEYVEDAA GTVIESIYPA TGEIIARLHA ATPGIVEKAI
60 70 80 90 100
AAAKRAQPEW AAMSPTARGR ILKRAAELMR QRNRELSELE TLDTGKPIQE
110 120 130 140 150
TIVADPTSGA DSFEFFGGVA PAALNGDYIP LGGDFAYTKR VPLGVCVGIG
160 170 180 190 200
AWNYPQQIAC WKGAPALVAG NAMVFKPSEN TPLGALKIAE ILIEAGLPKG
210 220 230 240 250
LFNVIQGDRA TGPLLVNHPD VAKVSLTGSV PTGKKVAGAA AAELKHVTME
260 270 280 290 300
LGGKSPLIVF DDADLESAIG GAMLGNFYST GQVCSNGTRV FVQRKIKEPF
310 320 330 340 350
LARLKERTEA IVIGDPLDEA TQLGPMVSAA QRDKVFSYIG KGKAEGARLV
360 370 380 390 400
TGGGIPNNVS GEGTYIQPTV FADVTDGMTI AREEIFGPVM CVLDFDDEVE
410 420 430 440 450
VIARANATEF GLSAGVFTAD LTRAHRVADR LEAGTLWINT YNLCPVEIPF
460 470 480
GGSKQSGFGR ENSVAALNHY TELKTVYVGM GPVEAPY
Length:487
Mass (Da):51,549
Last modified:May 30, 2000 - v2
Checksum:iD548CDF0BB1329AA
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti82 – 821R → L in AAC13368. (PubMed:9141699)Curated
Sequence conflicti374 – 3741V → D in AAC13368. (PubMed:9141699)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U39940 Genomic DNA. Translation: AAC13368.1.
AL591688 Genomic DNA. Translation: CAC45520.1.
RefSeqiNP_385054.1. NC_003047.1.

Genome annotation databases

EnsemblBacteriaiCAC45520; CAC45520; SMc00094.
GeneIDi1232591.
KEGGisme:SMc00094.
PATRICi23631171. VBISinMel96828_2347.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U39940 Genomic DNA. Translation: AAC13368.1.
AL591688 Genomic DNA. Translation: CAC45520.1.
RefSeqiNP_385054.1. NC_003047.1.

3D structure databases

ProteinModelPortaliP54222.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi266834.SMc00094.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAC45520; CAC45520; SMc00094.
GeneIDi1232591.
KEGGisme:SMc00094.
PATRICi23631171. VBISinMel96828_2347.

Phylogenomic databases

eggNOGiCOG1012.
HOGENOMiHOG000271505.
KOiK00130.
OMAiHVYQNIA.
OrthoDBiEOG6BS8QW.

Enzyme and pathway databases

UniPathwayiUPA00529; UER00386.
BioCyciMetaCyc:MONOMER-246.
SMEL266834:GJF6-971-MONOMER.

Family and domain databases

Gene3Di3.40.309.10. 1 hit.
3.40.605.10. 1 hit.
HAMAPiMF_00804. BADH.
InterProiIPR016161. Ald_DH/histidinol_DH.
IPR016163. Ald_DH_C.
IPR016160. Ald_DH_CS_CYS.
IPR029510. Ald_DH_CS_GLU.
IPR016162. Ald_DH_N.
IPR015590. Aldehyde_DH_dom.
IPR011264. BADH.
[Graphical view]
PfamiPF00171. Aldedh. 1 hit.
[Graphical view]
SUPFAMiSSF53720. SSF53720. 1 hit.
TIGRFAMsiTIGR01804. BADH. 1 hit.
PROSITEiPS00070. ALDEHYDE_DEHYDR_CYS. 1 hit.
PS00687. ALDEHYDE_DEHYDR_GLU. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular characterization of the bet genes encoding glycine betaine synthesis in Sinorhizobium meliloti 102F34."
    Pocard J.A., Vincent N., Boncompagni E., Tombras Smith L., Poggi M.-C., Le Rudulier D.
    Microbiology 143:1369-1379(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 102F34.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 1021.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 1021.

Entry informationi

Entry nameiBETB1_RHIME
AccessioniPrimary (citable) accession number: P54222
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: May 30, 2000
Last modified: January 7, 2015
This is version 96 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.