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P54222

- BETB1_RHIME

UniProt

P54222 - BETB1_RHIME

Protein

NAD/NADP-dependent betaine aldehyde dehydrogenase 1

Gene

betB1

Organism
Rhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium meliloti)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 94 (01 Oct 2014)
      Sequence version 2 (30 May 2000)
      Previous versions | rss
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    • Comment

    Functioni

    Involved in the biosynthesis of the osmoprotectant glycine betaine. Catalyzes the reversible oxidation of betaine aldehyde to the corresponding acid.UniRule annotation

    Catalytic activityi

    Betaine aldehyde + NAD+ + H2O = betaine + NADH.UniRule annotation

    Cofactori

    Binds 2 potassium ions per subunit.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi26 – 261Potassium 1UniRule annotation
    Metal bindingi27 – 271Potassium 1; via carbonyl oxygenUniRule annotation
    Metal bindingi93 – 931Potassium 1UniRule annotation
    Active sitei162 – 1621Charge relay systemUniRule annotation
    Metal bindingi244 – 2441Potassium 2; via carbonyl oxygenUniRule annotation
    Active sitei250 – 2501Proton acceptorUniRule annotation
    Binding sitei284 – 2841NAD/NADPUniRule annotation
    Binding sitei384 – 3841NAD/NADPUniRule annotation
    Metal bindingi454 – 4541Potassium 2; via carbonyl oxygenUniRule annotation
    Metal bindingi457 – 4571Potassium 2; via carbonyl oxygenUniRule annotation
    Active sitei461 – 4611Charge relay systemUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi150 – 1534NAD/NADPUniRule annotation
    Nucleotide bindingi176 – 1794NAD/NADPUniRule annotation
    Nucleotide bindingi228 – 2336NAD/NADPUniRule annotation

    GO - Molecular functioni

    1. betaine-aldehyde dehydrogenase activity Source: UniProtKB-HAMAP
    2. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. glycine betaine biosynthetic process from choline Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Ligandi

    Metal-binding, NAD, NADP, Potassium

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-246.
    SMEL266834:GJF6-971-MONOMER.
    UniPathwayiUPA00529; UER00386.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    NAD/NADP-dependent betaine aldehyde dehydrogenase 1UniRule annotation (EC:1.2.1.8UniRule annotation)
    Short name:
    BADH 1UniRule annotation
    Gene namesi
    Name:betB1UniRule annotation
    Synonyms:betB
    Ordered Locus Names:R00948
    ORF Names:SMc00094
    OrganismiRhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium meliloti)
    Taxonomic identifieri266834 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeSinorhizobium/Ensifer groupSinorhizobium
    ProteomesiUP000001976: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 487487NAD/NADP-dependent betaine aldehyde dehydrogenase 1PRO_0000056553Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei284 – 2841Cysteine sulfenic acid (-SOH)UniRule annotation

    Keywords - PTMi

    Oxidation

    Proteomic databases

    ProMEXiP54222.

    Interactioni

    Subunit structurei

    Dimer of dimers.UniRule annotation

    Protein-protein interaction databases

    STRINGi266834.SMc00094.

    Structurei

    3D structure databases

    ProteinModelPortaliP54222.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the aldehyde dehydrogenase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG1012.
    HOGENOMiHOG000271505.
    KOiK00130.
    OMAiSIFEARW.
    OrthoDBiEOG6BS8QW.

    Family and domain databases

    Gene3Di3.40.309.10. 1 hit.
    3.40.605.10. 1 hit.
    HAMAPiMF_00804. BADH.
    InterProiIPR016161. Ald_DH/histidinol_DH.
    IPR016163. Ald_DH_C.
    IPR016160. Ald_DH_CS_CYS.
    IPR029510. Ald_DH_CS_GLU.
    IPR016162. Ald_DH_N.
    IPR015590. Aldehyde_DH_dom.
    IPR011264. BADH.
    [Graphical view]
    PfamiPF00171. Aldedh. 1 hit.
    [Graphical view]
    SUPFAMiSSF53720. SSF53720. 1 hit.
    TIGRFAMsiTIGR01804. BADH. 1 hit.
    PROSITEiPS00070. ALDEHYDE_DEHYDR_CYS. 1 hit.
    PS00687. ALDEHYDE_DEHYDR_GLU. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P54222-1 [UniParc]FASTAAdd to Basket

    « Hide

    MRAQPKASHF IDGEYVEDAA GTVIESIYPA TGEIIARLHA ATPGIVEKAI    50
    AAAKRAQPEW AAMSPTARGR ILKRAAELMR QRNRELSELE TLDTGKPIQE 100
    TIVADPTSGA DSFEFFGGVA PAALNGDYIP LGGDFAYTKR VPLGVCVGIG 150
    AWNYPQQIAC WKGAPALVAG NAMVFKPSEN TPLGALKIAE ILIEAGLPKG 200
    LFNVIQGDRA TGPLLVNHPD VAKVSLTGSV PTGKKVAGAA AAELKHVTME 250
    LGGKSPLIVF DDADLESAIG GAMLGNFYST GQVCSNGTRV FVQRKIKEPF 300
    LARLKERTEA IVIGDPLDEA TQLGPMVSAA QRDKVFSYIG KGKAEGARLV 350
    TGGGIPNNVS GEGTYIQPTV FADVTDGMTI AREEIFGPVM CVLDFDDEVE 400
    VIARANATEF GLSAGVFTAD LTRAHRVADR LEAGTLWINT YNLCPVEIPF 450
    GGSKQSGFGR ENSVAALNHY TELKTVYVGM GPVEAPY 487
    Length:487
    Mass (Da):51,549
    Last modified:May 30, 2000 - v2
    Checksum:iD548CDF0BB1329AA
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti82 – 821R → L in AAC13368. (PubMed:9141699)Curated
    Sequence conflicti374 – 3741V → D in AAC13368. (PubMed:9141699)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U39940 Genomic DNA. Translation: AAC13368.1.
    AL591688 Genomic DNA. Translation: CAC45520.1.
    RefSeqiNP_385054.1. NC_003047.1.

    Genome annotation databases

    EnsemblBacteriaiCAC45520; CAC45520; SMc00094.
    GeneIDi1232591.
    KEGGisme:SMc00094.
    PATRICi23631171. VBISinMel96828_2347.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U39940 Genomic DNA. Translation: AAC13368.1 .
    AL591688 Genomic DNA. Translation: CAC45520.1 .
    RefSeqi NP_385054.1. NC_003047.1.

    3D structure databases

    ProteinModelPortali P54222.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 266834.SMc00094.

    Proteomic databases

    ProMEXi P54222.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAC45520 ; CAC45520 ; SMc00094 .
    GeneIDi 1232591.
    KEGGi sme:SMc00094.
    PATRICi 23631171. VBISinMel96828_2347.

    Phylogenomic databases

    eggNOGi COG1012.
    HOGENOMi HOG000271505.
    KOi K00130.
    OMAi SIFEARW.
    OrthoDBi EOG6BS8QW.

    Enzyme and pathway databases

    UniPathwayi UPA00529 ; UER00386 .
    BioCyci MetaCyc:MONOMER-246.
    SMEL266834:GJF6-971-MONOMER.

    Family and domain databases

    Gene3Di 3.40.309.10. 1 hit.
    3.40.605.10. 1 hit.
    HAMAPi MF_00804. BADH.
    InterProi IPR016161. Ald_DH/histidinol_DH.
    IPR016163. Ald_DH_C.
    IPR016160. Ald_DH_CS_CYS.
    IPR029510. Ald_DH_CS_GLU.
    IPR016162. Ald_DH_N.
    IPR015590. Aldehyde_DH_dom.
    IPR011264. BADH.
    [Graphical view ]
    Pfami PF00171. Aldedh. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53720. SSF53720. 1 hit.
    TIGRFAMsi TIGR01804. BADH. 1 hit.
    PROSITEi PS00070. ALDEHYDE_DEHYDR_CYS. 1 hit.
    PS00687. ALDEHYDE_DEHYDR_GLU. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular characterization of the bet genes encoding glycine betaine synthesis in Sinorhizobium meliloti 102F34."
      Pocard J.A., Vincent N., Boncompagni E., Tombras Smith L., Poggi M.-C., Le Rudulier D.
      Microbiology 143:1369-1379(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: 102F34.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 1021.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 1021.

    Entry informationi

    Entry nameiBETB1_RHIME
    AccessioniPrimary (citable) accession number: P54222
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: May 30, 2000
    Last modified: October 1, 2014
    This is version 94 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3