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P54219 (VMAT1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 125. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Chromaffin granule amine transporter
Alternative name(s):
Solute carrier family 18 member 1
Vesicular amine transporter 1
Short name=VAT1
Gene names
Name:SLC18A1
Synonyms:VAT1, VMAT1
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length525 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Involved in the transport of biogenic monoamines, such as serotonin, from the cytoplasm into the secretory vesicles of neuroendocrine and endocrine cells. Ref.4

Subcellular location

Isoform 1: Cytoplasmic vesicle membrane; Multi-pass membrane protein Ref.4.

Isoform 2: Endoplasmic reticulum membrane; Multi-pass membrane protein Ref.4.

Sequence similarities

Belongs to the major facilitator superfamily. Vesicular transporter family.

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: P54219-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: P54219-2)

Also known as: VMAT1delta15;

The sequence of this isoform differs from the canonical sequence as follows:
     445-525: PSTGGAIVKA...DSDEEPDHEE → YSESGLPHGDPDVCNPEAHEGISSGGGQ
Note: Unable to uptake serotonin.
Isoform 3 (identifier: P54219-3)

The sequence of this isoform differs from the canonical sequence as follows:
     307-338: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 525525Chromaffin granule amine transporter
PRO_0000127510

Regions

Topological domain1 – 2121Cytoplasmic Potential
Transmembrane22 – 4221Helical; Potential
Topological domain43 – 13896Lumenal, vesicle Potential
Transmembrane139 – 15820Helical; Potential
Topological domain159 – 1679Cytoplasmic Potential
Transmembrane168 – 18821Helical; Potential
Topological domain189 – 1979Lumenal, vesicle Potential
Transmembrane198 – 21821Helical; Potential
Topological domain219 – 2279Cytoplasmic Potential
Transmembrane228 – 25023Helical; Potential
Topological domain251 – 2566Lumenal, vesicle Potential
Transmembrane257 – 27923Helical; Potential
Topological domain280 – 29920Cytoplasmic Potential
Transmembrane300 – 31920Helical; Potential
Topological domain320 – 33516Lumenal, vesicle Potential
Transmembrane336 – 36025Helical; Potential
Topological domain361 – 3655Cytoplasmic Potential
Transmembrane366 – 38621Helical; Potential
Topological domain387 – 39711Lumenal, vesicle Potential
Transmembrane398 – 41821Helical; Potential
Topological domain419 – 4224Cytoplasmic Potential
Transmembrane423 – 44321Helical; Potential
Topological domain444 – 4485Lumenal, vesicle Potential
Transmembrane449 – 47022Helical; Potential
Topological domain471 – 52555Cytoplasmic Potential

Amino acid modifications

Glycosylation581N-linked (GlcNAc...) Potential
Glycosylation871N-linked (GlcNAc...) Potential
Glycosylation1041N-linked (GlcNAc...) Potential

Natural variations

Alternative sequence307 – 33832Missing in isoform 3.
VSP_046304
Alternative sequence445 – 52581PSTGG…PDHEE → YSESGLPHGDPDVCNPEAHE GISSGGGQ in isoform 2.
VSP_046305
Natural variant41T → P. Ref.3
Corresponds to variant rs2270641 [ dbSNP | Ensembl ].
VAR_021856
Natural variant111R → Q.
Corresponds to variant rs17092144 [ dbSNP | Ensembl ].
VAR_052002
Natural variant741A → V.
Corresponds to variant rs17215815 [ dbSNP | Ensembl ].
VAR_024632
Natural variant821F → C.
Corresponds to variant rs17215822 [ dbSNP | Ensembl ].
VAR_029149
Natural variant841F → S.
Corresponds to variant rs17215801 [ dbSNP | Ensembl ].
VAR_024633
Natural variant981S → T.
Corresponds to variant rs2270637 [ dbSNP | Ensembl ].
VAR_020033
Natural variant1011A → P.
Corresponds to variant rs17222218 [ dbSNP | Ensembl ].
VAR_024634
Natural variant1361I → T. Ref.3
Corresponds to variant rs1390938 [ dbSNP | Ensembl ].
VAR_012065
Natural variant1401G → R.
Corresponds to variant rs17215808 [ dbSNP | Ensembl ].
VAR_029150
Natural variant1641I → M.
Corresponds to variant rs17222092 [ dbSNP | Ensembl ].
VAR_024635
Natural variant2021I → T.
Corresponds to variant rs17222120 [ dbSNP | Ensembl ].
VAR_029151
Natural variant2491V → I.
Corresponds to variant rs17215759 [ dbSNP | Ensembl ].
VAR_024636
Natural variant3921L → V.
Corresponds to variant rs17092104 [ dbSNP | Ensembl ].
VAR_024637

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: 041F2959F4F2BFBC

FASTA52556,257
        10         20         30         40         50         60 
MLRTILDAPQ RLLKEGRASR QLVLVVVFVA LLLDNMLFTV VVPIVPTFLY DMEFKEVNSS 

        70         80         90        100        110        120 
LHLGHAGSSP HALASPAFST IFSFFNNNTV AVEESVPSGI AWMNDTASTI PPPATEAISA 

       130        140        150        160        170        180 
HKNNCLQGTG FLEEEITRVG VLFASKAVMQ LLVNPFVGPL TNRIGYHIPM FAGFVIMFLS 

       190        200        210        220        230        240 
TVMFAFSGTY TLLFVARTLQ GIGSSFSSVA GLGMLASVYT DDHERGRAMG TALGGLALGL 

       250        260        270        280        290        300 
LVGAPFGSVM YEFVGKSAPF LILAFLALLD GALQLCILQP SKVSPESAKG TPLFMLLKDP 

       310        320        330        340        350        360 
YILVAAGSIC FANMGVAILE PTLPIWMMQT MCSPKWQLGL AFLPASVSYL IGTNLFGVLA 

       370        380        390        400        410        420 
NKMGRWLCSL IGMLVVGTSL LCVPLAHNIF GLIGPNAGLG LAIGMVDSSM MPIMGHLVDL 

       430        440        450        460        470        480 
RHTSVYGSVY AIADVAFCMG FAIGPSTGGA IVKAIGFPWL MVITGVINIV YAPLCYYLRS 

       490        500        510        520 
PPAKEEKLAI LSQDCPMETR MYATQKPTKE FPLGEDSDEE PDHEE 

« Hide

Isoform 2 (VMAT1delta15) [UniParc].

Checksum: AC98F90178CFEDC1
Show »

FASTA47250,026
Isoform 3 [UniParc].

Checksum: 95F336015020D3B5
Show »

FASTA49352,677

References

« Hide 'large scale' references
[1]"Distinct pharmacological properties and distribution in neurons and endocrine cells of two isoforms of the human vesicular monoamine transporter."
Erickson J.D., Schaefer M.K.-H., Bonner T.I., Eiden L.E., Weihe E.
Proc. Natl. Acad. Sci. U.S.A. 93:5166-5171(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
Tissue: Pheochromocytoma.
[2]"DNA sequence and analysis of human chromosome 8."
Nusbaum C., Mikkelsen T.S., Zody M.C., Asakawa S., Taudien S., Garber M., Kodira C.D., Schueler M.G., Shimizu A., Whittaker C.A., Chang J.L., Cuomo C.A., Dewar K., FitzGerald M.G., Yang X., Allen N.R., Anderson S., Asakawa T. expand/collapse author list , Blechschmidt K., Bloom T., Borowsky M.L., Butler J., Cook A., Corum B., DeArellano K., DeCaprio D., Dooley K.T., Dorris L. III, Engels R., Gloeckner G., Hafez N., Hagopian D.S., Hall J.L., Ishikawa S.K., Jaffe D.B., Kamat A., Kudoh J., Lehmann R., Lokitsang T., Macdonald P., Major J.E., Matthews C.D., Mauceli E., Menzel U., Mihalev A.H., Minoshima S., Murayama Y., Naylor J.W., Nicol R., Nguyen C., O'Leary S.B., O'Neill K., Parker S.C.J., Polley A., Raymond C.K., Reichwald K., Rodriguez J., Sasaki T., Schilhabel M., Siddiqui R., Smith C.L., Sneddon T.P., Talamas J.A., Tenzin P., Topham K., Venkataraman V., Wen G., Yamazaki S., Young S.K., Zeng Q., Zimmer A.R., Rosenthal A., Birren B.W., Platzer M., Shimizu N., Lander E.S.
Nature 439:331-335(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3), VARIANTS PRO-4 AND THR-136.
Tissue: Brain.
[4]"Identification and characterization of a novel splicing variant of vesicular monoamine transporter 1."
Essand M., Vikman S., Grawe J., Gedda L., Hellberg C., Oberg K., Totterman T.H., Giandomenico V.
J. Mol. Endocrinol. 35:489-501(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 445-472 (ISOFORM 2), FUNCTION, ALTERNATIVE SPLICING, SUBCELLULAR LOCATION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U39905 mRNA. Translation: AAC50472.1.
AC025853 Genomic DNA. No translation available.
BC006317 mRNA. Translation: AAH06317.1.
RefSeqNP_001129163.1. NM_001135691.2.
NP_001135796.1. NM_001142324.1.
NP_001135797.1. NM_001142325.1.
NP_003044.1. NM_003053.3.
UniGeneHs.158322.

3D structure databases

ProteinModelPortalP54219.
SMRP54219. Positions 144-182.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid112458. 2 interactions.
STRING9606.ENSP00000276373.

Chemistry

BindingDBP54219.
ChEMBLCHEMBL1838.
GuidetoPHARMACOLOGY1011.

Protein family/group databases

TCDB2.A.1.2.12. the major facilitator superfamily (mfs).

PTM databases

PhosphoSiteP54219.

Polymorphism databases

DMDM1722741.

Proteomic databases

PaxDbP54219.
PRIDEP54219.

Protocols and materials databases

DNASU6570.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000265808; ENSP00000265808; ENSG00000036565. [P54219-3]
ENST00000276373; ENSP00000276373; ENSG00000036565. [P54219-1]
ENST00000381608; ENSP00000371021; ENSG00000036565. [P54219-2]
ENST00000437980; ENSP00000413361; ENSG00000036565. [P54219-2]
ENST00000440926; ENSP00000387549; ENSG00000036565. [P54219-1]
ENST00000519026; ENSP00000429664; ENSG00000036565. [P54219-3]
GeneID6570.
KEGGhsa:6570.
UCSCuc003wzm.3. human. [P54219-1]
uc003wzn.3. human.

Organism-specific databases

CTD6570.
GeneCardsGC08M020046.
HGNCHGNC:10934. SLC18A1.
HPAHPA007210.
MIM193002. gene.
neXtProtNX_P54219.
PharmGKBPA324.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG255549.
HOGENOMHOG000018545.
HOVERGENHBG055082.
InParanoidP54219.
KOK08155.
OMANNCLQGT.
OrthoDBEOG79CXZ7.
PhylomeDBP54219.
TreeFamTF313494.

Enzyme and pathway databases

ReactomeREACT_15518. Transmembrane transport of small molecules.
REACT_20679. Amine compound SLC transporters.

Gene expression databases

ArrayExpressP54219.
BgeeP54219.
CleanExHS_SLC18A1.
HS_VAT1.
GenevestigatorP54219.

Family and domain databases

InterProIPR011701. MFS.
IPR020846. MFS_dom.
IPR016196. MFS_dom_general_subst_transpt.
IPR004734. Multidrug-R.
[Graphical view]
PfamPF07690. MFS_1. 1 hit.
[Graphical view]
SUPFAMSSF103473. SSF103473. 2 hits.
TIGRFAMsTIGR00880. 2_A_01_02. 1 hit.
PROSITEPS50850. MFS. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GenomeRNAi6570.
NextBio25563.
PROP54219.
SOURCESearch...

Entry information

Entry nameVMAT1_HUMAN
AccessionPrimary (citable) accession number: P54219
Secondary accession number(s): E9PDJ5, Q9BRE4
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: April 16, 2014
This is version 125 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 8

Human chromosome 8: entries, gene names and cross-references to MIM