P54219 (VMAT1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Chromaffin granule amine transporter

Alternative name(s):
Solute carrier family 18 member 1
Vesicular amine transporter 1
Short name=VAT1

Gene names

Name:	SLC18A1
Synonyms:	VAT1, VMAT1

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	525 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Involved in the transport of biogenic monoamines, such as serotonin, from the cytoplasm into the secretory vesicles of neuroendocrine and endocrine cells. Ref.4
Subcellular location	Isoform 1: Cytoplasmic vesicle membrane; Multi-pass membrane protein Ref.4. Isoform 2: Endoplasmic reticulum membrane; Multi-pass membrane protein Ref.4.
Sequence similarities	Belongs to the major facilitator superfamily. Vesicular transporter family.

Ontologies

Keywords
Biological process	Neurotransmitter transport Transport
Cellular component	Cytoplasmic vesicle Endoplasmic reticulum Membrane
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Transmembrane Transmembrane helix
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	drug transmembrane transport Inferred from electronic annotation. Source: GOC neurotransmitter transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	clathrin-sculpted monoamine transport vesicle membrane Traceable author statement. Source: Reactome endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	drug transmembrane transporter activity Inferred from electronic annotation. Source: InterPro monoamine transmembrane transporter activity Traceable author statement Ref.1. Source: UniProtKB
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: P54219-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: P54219-2)

Also known as: VMAT1delta15;

The sequence of this isoform differs from the canonical sequence as follows:
     445-525: PSTGGAIVKA...DSDEEPDHEE → YSESGLPHGDPDVCNPEAHEGISSGGGQ

Note: Unable to uptake serotonin.

Isoform 3 (identifier: P54219-3)

The sequence of this isoform differs from the canonical sequence as follows:
     307-338: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 525	525	Chromaffin granule amine transporter		PRO_0000127510
Regions
	Topological domain	1 – 21	21	Cytoplasmic Potential
	Transmembrane	22 – 42	21	Helical; Potential
	Topological domain	43 – 138	96	Lumenal, vesicle Potential
	Transmembrane	139 – 158	20	Helical; Potential
	Topological domain	159 – 167	9	Cytoplasmic Potential
	Transmembrane	168 – 188	21	Helical; Potential
	Topological domain	189 – 197	9	Lumenal, vesicle Potential
	Transmembrane	198 – 218	21	Helical; Potential
	Topological domain	219 – 227	9	Cytoplasmic Potential
	Transmembrane	228 – 250	23	Helical; Potential
	Topological domain	251 – 256	6	Lumenal, vesicle Potential
	Transmembrane	257 – 279	23	Helical; Potential
	Topological domain	280 – 299	20	Cytoplasmic Potential
	Transmembrane	300 – 319	20	Helical; Potential
	Topological domain	320 – 335	16	Lumenal, vesicle Potential
	Transmembrane	336 – 360	25	Helical; Potential
	Topological domain	361 – 365	5	Cytoplasmic Potential
	Transmembrane	366 – 386	21	Helical; Potential
	Topological domain	387 – 397	11	Lumenal, vesicle Potential
	Transmembrane	398 – 418	21	Helical; Potential
	Topological domain	419 – 422	4	Cytoplasmic Potential
	Transmembrane	423 – 443	21	Helical; Potential
	Topological domain	444 – 448	5	Lumenal, vesicle Potential
	Transmembrane	449 – 470	22	Helical; Potential
	Topological domain	471 – 525	55	Cytoplasmic Potential
Amino acid modifications
	Glycosylation	58	1	N-linked (GlcNAc...) Potential
	Glycosylation	87	1	N-linked (GlcNAc...) Potential
	Glycosylation	104	1	N-linked (GlcNAc...) Potential
Natural variations
	Alternative sequence	307 – 338	32	Missing in isoform 3.		VSP_046304
	Alternative sequence	445 – 525	81	PSTGG…PDHEE → YSESGLPHGDPDVCNPEAHE GISSGGGQ in isoform 2.		VSP_046305
	Natural variant	4	1	T → P. Ref.3 Corresponds to variant rs2270641 [ dbSNP | Ensembl ].		VAR_021856
	Natural variant	11	1	R → Q. Corresponds to variant rs17092144 [ dbSNP | Ensembl ].		VAR_052002
	Natural variant	74	1	A → V. Corresponds to variant rs17215815 [ dbSNP | Ensembl ].		VAR_024632
	Natural variant	82	1	F → C. Corresponds to variant rs17215822 [ dbSNP | Ensembl ].		VAR_029149
	Natural variant	84	1	F → S. Corresponds to variant rs17215801 [ dbSNP | Ensembl ].		VAR_024633
	Natural variant	98	1	S → T. Corresponds to variant rs2270637 [ dbSNP | Ensembl ].		VAR_020033
	Natural variant	101	1	A → P. Corresponds to variant rs17222218 [ dbSNP | Ensembl ].		VAR_024634
	Natural variant	136	1	I → T. Ref.3 Corresponds to variant rs1390938 [ dbSNP | Ensembl ].		VAR_012065
	Natural variant	140	1	G → R. Corresponds to variant rs17215808 [ dbSNP | Ensembl ].		VAR_029150
	Natural variant	164	1	I → M. Corresponds to variant rs17222092 [ dbSNP | Ensembl ].		VAR_024635
	Natural variant	202	1	I → T. Corresponds to variant rs17222120 [ dbSNP | Ensembl ].		VAR_029151
	Natural variant	249	1	V → I. Corresponds to variant rs17215759 [ dbSNP | Ensembl ].		VAR_024636
	Natural variant	392	1	L → V. Corresponds to variant rs17092104 [ dbSNP | Ensembl ].		VAR_024637

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 041F2959F4F2BFBC	FASTA	525	56,257
	10 20 30 40 50 60 MLRTILDAPQ RLLKEGRASR QLVLVVVFVA LLLDNMLFTV VVPIVPTFLY DMEFKEVNSS 70 80 90 100 110 120 LHLGHAGSSP HALASPAFST IFSFFNNNTV AVEESVPSGI AWMNDTASTI PPPATEAISA 130 140 150 160 170 180 HKNNCLQGTG FLEEEITRVG VLFASKAVMQ LLVNPFVGPL TNRIGYHIPM FAGFVIMFLS 190 200 210 220 230 240 TVMFAFSGTY TLLFVARTLQ GIGSSFSSVA GLGMLASVYT DDHERGRAMG TALGGLALGL 250 260 270 280 290 300 LVGAPFGSVM YEFVGKSAPF LILAFLALLD GALQLCILQP SKVSPESAKG TPLFMLLKDP 310 320 330 340 350 360 YILVAAGSIC FANMGVAILE PTLPIWMMQT MCSPKWQLGL AFLPASVSYL IGTNLFGVLA 370 380 390 400 410 420 NKMGRWLCSL IGMLVVGTSL LCVPLAHNIF GLIGPNAGLG LAIGMVDSSM MPIMGHLVDL 430 440 450 460 470 480 RHTSVYGSVY AIADVAFCMG FAIGPSTGGA IVKAIGFPWL MVITGVINIV YAPLCYYLRS 490 500 510 520 PPAKEEKLAI LSQDCPMETR MYATQKPTKE FPLGEDSDEE PDHEE
	Isoform 2 (VMAT1delta15) [UniParc]. Checksum: AC98F90178CFEDC1	FASTA	472	50,026
	10 20 30 40 50 60 MLRTILDAPQ RLLKEGRASR QLVLVVVFVA LLLDNMLFTV VVPIVPTFLY DMEFKEVNSS 70 80 90 100 110 120 LHLGHAGSSP HALASPAFST IFSFFNNNTV AVEESVPSGI AWMNDTASTI PPPATEAISA 130 140 150 160 170 180 HKNNCLQGTG FLEEEITRVG VLFASKAVMQ LLVNPFVGPL TNRIGYHIPM FAGFVIMFLS 190 200 210 220 230 240 TVMFAFSGTY TLLFVARTLQ GIGSSFSSVA GLGMLASVYT DDHERGRAMG TALGGLALGL 250 260 270 280 290 300 LVGAPFGSVM YEFVGKSAPF LILAFLALLD GALQLCILQP SKVSPESAKG TPLFMLLKDP 310 320 330 340 350 360 YILVAAGSIC FANMGVAILE PTLPIWMMQT MCSPKWQLGL AFLPASVSYL IGTNLFGVLA 370 380 390 400 410 420 NKMGRWLCSL IGMLVVGTSL LCVPLAHNIF GLIGPNAGLG LAIGMVDSSM MPIMGHLVDL 430 440 450 460 470 RHTSVYGSVY AIADVAFCMG FAIGYSESGL PHGDPDVCNP EAHEGISSGG GQ
	Isoform 3 [UniParc]. Checksum: 95F336015020D3B5	FASTA	493	52,677
	10 20 30 40 50 60 MLRTILDAPQ RLLKEGRASR QLVLVVVFVA LLLDNMLFTV VVPIVPTFLY DMEFKEVNSS 70 80 90 100 110 120 LHLGHAGSSP HALASPAFST IFSFFNNNTV AVEESVPSGI AWMNDTASTI PPPATEAISA 130 140 150 160 170 180 HKNNCLQGTG FLEEEITRVG VLFASKAVMQ LLVNPFVGPL TNRIGYHIPM FAGFVIMFLS 190 200 210 220 230 240 TVMFAFSGTY TLLFVARTLQ GIGSSFSSVA GLGMLASVYT DDHERGRAMG TALGGLALGL 250 260 270 280 290 300 LVGAPFGSVM YEFVGKSAPF LILAFLALLD GALQLCILQP SKVSPESAKG TPLFMLLKDP 310 320 330 340 350 360 YILVAAGLAF LPASVSYLIG TNLFGVLANK MGRWLCSLIG MLVVGTSLLC VPLAHNIFGL 370 380 390 400 410 420 IGPNAGLGLA IGMVDSSMMP IMGHLVDLRH TSVYGSVYAI ADVAFCMGFA IGPSTGGAIV 430 440 450 460 470 480 KAIGFPWLMV ITGVINIVYA PLCYYLRSPP AKEEKLAILS QDCPMETRMY ATQKPTKEFP 490 LGEDSDEEPD HEE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Distinct pharmacological properties and distribution in neurons and endocrine cells of two isoforms of the human vesicular monoamine transporter." Erickson J.D., Schaefer M.K.-H., Bonner T.I., Eiden L.E., Weihe E. Proc. Natl. Acad. Sci. U.S.A. 93:5166-5171(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Tissue: Pheochromocytoma.
[2]	"DNA sequence and analysis of human chromosome 8." Nusbaum C., Mikkelsen T.S., Zody M.C., Asakawa S., Taudien S., Garber M., Kodira C.D., Schueler M.G., Shimizu A., Whittaker C.A., Chang J.L., Cuomo C.A., Dewar K., FitzGerald M.G., Yang X., Allen N.R., Anderson S., Asakawa T. , Blechschmidt K., Bloom T., Borowsky M.L., Butler J., Cook A., Corum B., DeArellano K., DeCaprio D., Dooley K.T., Dorris L. III, Engels R., Gloeckner G., Hafez N., Hagopian D.S., Hall J.L., Ishikawa S.K., Jaffe D.B., Kamat A., Kudoh J., Lehmann R., Lokitsang T., Macdonald P., Major J.E., Matthews C.D., Mauceli E., Menzel U., Mihalev A.H., Minoshima S., Murayama Y., Naylor J.W., Nicol R., Nguyen C., O'Leary S.B., O'Neill K., Parker S.C.J., Polley A., Raymond C.K., Reichwald K., Rodriguez J., Sasaki T., Schilhabel M., Siddiqui R., Smith C.L., Sneddon T.P., Talamas J.A., Tenzin P., Topham K., Venkataraman V., Wen G., Yamazaki S., Young S.K., Zeng Q., Zimmer A.R., Rosenthal A., Birren B.W., Platzer M., Shimizu N., Lander E.S. Nature 439:331-335(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3), VARIANTS PRO-4 AND THR-136. Tissue: Brain.
[4]	"Identification and characterization of a novel splicing variant of vesicular monoamine transporter 1." Essand M., Vikman S., Grawe J., Gedda L., Hellberg C., Oberg K., Totterman T.H., Giandomenico V. J. Mol. Endocrinol. 35:489-501(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 445-472 (ISOFORM 2), FUNCTION, ALTERNATIVE SPLICING, SUBCELLULAR LOCATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U39905 mRNA. Translation: AAC50472.1. AC025853 Genomic DNA. No translation available. BC006317 mRNA. Translation: AAH06317.1.
IPI	IPI00005423. IPI00332277.
RefSeq	NP_001129163.1. NM_001135691.2. NP_001135796.1. NM_001142324.1. NP_001135797.1. NM_001142325.1. NP_003044.1. NM_003053.3.
UniGene	Hs.158322.
3D structure databases
ProteinModelPortal	P54219.
ModBase	Search...
Protein-protein interaction databases
STRING	9606.ENSP00000276373.
Protein family/group databases
TCDB	2.A.1.2.12. major facilitator superfamily (MFS).
PTM databases
PhosphoSite	P54219.
Polymorphism databases
DMDM	1722741.
Proteomic databases
PaxDb	P54219.
PRIDE	P54219.
Protocols and materials databases
DNASU	6570.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000265808; ENSP00000265808; ENSG00000036565. ENST00000276373; ENSP00000276373; ENSG00000036565. ENST00000381608; ENSP00000371021; ENSG00000036565. ENST00000437980; ENSP00000413361; ENSG00000036565. ENST00000440926; ENSP00000387549; ENSG00000036565. ENST00000519026; ENSP00000429664; ENSG00000036565.
GeneID	6570.
KEGG	hsa:6570.
UCSC	uc003wzm.3. human.
Organism-specific databases
CTD	6570.
GeneCards	GC08M020046.
HGNC	HGNC:10934. SLC18A1.
HPA	HPA007210.
MIM	193002. gene.
neXtProt	NX_P54219.
PharmGKB	PA324.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG255549.
HOGENOM	HOG000018545.
HOVERGEN	HBG055082.
InParanoid	P54219.
KO	K08155.
OMA	CPMETRM.
OrthoDB	EOG4DR9C5.
PhylomeDB	P54219.
Enzyme and pathway databases
Reactome	REACT_15518. Transmembrane transport of small molecules. REACT_20679. Amine compound SLC transporters.
Gene expression databases
ArrayExpress	P54219.
Bgee	P54219.
CleanEx	HS_SLC18A1. HS_VAT1.
Genevestigator	P54219.
GermOnline	ENSG00000036565. Homo sapiens.
Family and domain databases
InterPro	IPR011701. MFS. IPR020846. MFS_dom. IPR016196. MFS_dom_general_subst_transpt. IPR004734. Multidrug-R. [Graphical view]
Pfam	PF07690. MFS_1. 1 hit. [Graphical view]
SUPFAM	SSF103473. MFS_gen_substrate_transporter. 1 hit.
TIGRFAMs	TIGR00880. 2_A_01_02. 1 hit.
PROSITE	PS50850. MFS. 1 hit. [Graphical view]
ProtoNet	Search...
Other
BindingDB	P54219.
ChEMBL	CHEMBL1838.
GenomeRNAi	6570.
NextBio	25563.
SOURCE	Search...

Entry information

Entry name

VMAT1_HUMAN

Accession

Primary (citable) accession number: P54219
Secondary accession number(s): E9PDJ5, Q9BRE4

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	May 1, 2013
This is version 114 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.