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Protein

Chromaffin granule amine transporter

Gene

SLC18A1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Involved in the transport of biogenic monoamines, such as serotonin, from the cytoplasm into the secretory vesicles of neuroendocrine and endocrine cells.1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Neurotransmitter transport, Transport

Enzyme and pathway databases

BioCyciZFISH:ENSG00000036565-MONOMER.
ReactomeiR-HSA-442660. Na+/Cl- dependent neurotransmitter transporters.

Protein family/group databases

TCDBi2.A.1.2.12. the major facilitator superfamily (mfs).

Names & Taxonomyi

Protein namesi
Recommended name:
Chromaffin granule amine transporter
Alternative name(s):
Solute carrier family 18 member 1
Vesicular amine transporter 1
Short name:
VAT1
Gene namesi
Name:SLC18A1
Synonyms:VAT1, VMAT1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 8

Organism-specific databases

HGNCiHGNC:10934. SLC18A1.

Subcellular locationi

Isoform 1 :
Isoform 2 :

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 21CytoplasmicSequence analysisAdd BLAST21
Transmembranei22 – 42HelicalSequence analysisAdd BLAST21
Topological domaini43 – 138Lumenal, vesicleSequence analysisAdd BLAST96
Transmembranei139 – 158HelicalSequence analysisAdd BLAST20
Topological domaini159 – 167CytoplasmicSequence analysis9
Transmembranei168 – 188HelicalSequence analysisAdd BLAST21
Topological domaini189 – 197Lumenal, vesicleSequence analysis9
Transmembranei198 – 218HelicalSequence analysisAdd BLAST21
Topological domaini219 – 227CytoplasmicSequence analysis9
Transmembranei228 – 250HelicalSequence analysisAdd BLAST23
Topological domaini251 – 256Lumenal, vesicleSequence analysis6
Transmembranei257 – 279HelicalSequence analysisAdd BLAST23
Topological domaini280 – 299CytoplasmicSequence analysisAdd BLAST20
Transmembranei300 – 319HelicalSequence analysisAdd BLAST20
Topological domaini320 – 335Lumenal, vesicleSequence analysisAdd BLAST16
Transmembranei336 – 360HelicalSequence analysisAdd BLAST25
Topological domaini361 – 365CytoplasmicSequence analysis5
Transmembranei366 – 386HelicalSequence analysisAdd BLAST21
Topological domaini387 – 397Lumenal, vesicleSequence analysisAdd BLAST11
Transmembranei398 – 418HelicalSequence analysisAdd BLAST21
Topological domaini419 – 422CytoplasmicSequence analysis4
Transmembranei423 – 443HelicalSequence analysisAdd BLAST21
Topological domaini444 – 448Lumenal, vesicleSequence analysis5
Transmembranei449 – 470HelicalSequence analysisAdd BLAST22
Topological domaini471 – 525CytoplasmicSequence analysisAdd BLAST55

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasmic vesicle, Endoplasmic reticulum, Membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi6570.
OpenTargetsiENSG00000036565.
PharmGKBiPA324.

Chemistry databases

ChEMBLiCHEMBL1838.
DrugBankiDB01363. Ephedra.
DB01577. Methamphetamine.
DB00368. Norepinephrine.
DB00206. Reserpine.
GuidetoPHARMACOLOGYi1011.

Polymorphism and mutation databases

BioMutaiSLC18A1.
DMDMi1722741.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001275101 – 525Chromaffin granule amine transporterAdd BLAST525

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi58N-linked (GlcNAc...)Sequence analysis1
Glycosylationi87N-linked (GlcNAc...)Sequence analysis1
Glycosylationi104N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiP54219.
PeptideAtlasiP54219.
PRIDEiP54219.

PTM databases

iPTMnetiP54219.
PhosphoSitePlusiP54219.

Expressioni

Gene expression databases

BgeeiENSG00000036565.
CleanExiHS_SLC18A1.
HS_VAT1.
ExpressionAtlasiP54219. baseline and differential.
GenevisibleiP54219. HS.

Interactioni

Protein-protein interaction databases

BioGridi112458. 56 interactors.
STRINGi9606.ENSP00000276373.

Chemistry databases

BindingDBiP54219.

Structurei

3D structure databases

ProteinModelPortaliP54219.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3764. Eukaryota.
ENOG410XW69. LUCA.
GeneTreeiENSGT00390000003851.
HOGENOMiHOG000018545.
HOVERGENiHBG055082.
InParanoidiP54219.
KOiK08155.
OMAiNNCLQGT.
OrthoDBiEOG091G0534.
PhylomeDBiP54219.
TreeFamiTF313494.

Family and domain databases

CDDicd06174. MFS. 1 hit.
InterProiIPR011701. MFS.
IPR020846. MFS_dom.
IPR004734. Multidrug-R.
[Graphical view]
PfamiPF07690. MFS_1. 1 hit.
[Graphical view]
SUPFAMiSSF103473. SSF103473. 2 hits.
TIGRFAMsiTIGR00880. 2_A_01_02. 1 hit.
PROSITEiPS50850. MFS. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P54219-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MLRTILDAPQ RLLKEGRASR QLVLVVVFVA LLLDNMLFTV VVPIVPTFLY
60 70 80 90 100
DMEFKEVNSS LHLGHAGSSP HALASPAFST IFSFFNNNTV AVEESVPSGI
110 120 130 140 150
AWMNDTASTI PPPATEAISA HKNNCLQGTG FLEEEITRVG VLFASKAVMQ
160 170 180 190 200
LLVNPFVGPL TNRIGYHIPM FAGFVIMFLS TVMFAFSGTY TLLFVARTLQ
210 220 230 240 250
GIGSSFSSVA GLGMLASVYT DDHERGRAMG TALGGLALGL LVGAPFGSVM
260 270 280 290 300
YEFVGKSAPF LILAFLALLD GALQLCILQP SKVSPESAKG TPLFMLLKDP
310 320 330 340 350
YILVAAGSIC FANMGVAILE PTLPIWMMQT MCSPKWQLGL AFLPASVSYL
360 370 380 390 400
IGTNLFGVLA NKMGRWLCSL IGMLVVGTSL LCVPLAHNIF GLIGPNAGLG
410 420 430 440 450
LAIGMVDSSM MPIMGHLVDL RHTSVYGSVY AIADVAFCMG FAIGPSTGGA
460 470 480 490 500
IVKAIGFPWL MVITGVINIV YAPLCYYLRS PPAKEEKLAI LSQDCPMETR
510 520
MYATQKPTKE FPLGEDSDEE PDHEE
Length:525
Mass (Da):56,257
Last modified:October 1, 1996 - v1
Checksum:i041F2959F4F2BFBC
GO
Isoform 2 (identifier: P54219-2) [UniParc]FASTAAdd to basket
Also known as: VMAT1delta15

The sequence of this isoform differs from the canonical sequence as follows:
     445-525: PSTGGAIVKA...DSDEEPDHEE → YSESGLPHGDPDVCNPEAHEGISSGGGQ

Note: Unable to uptake serotonin.
Show »
Length:472
Mass (Da):50,026
Checksum:iAC98F90178CFEDC1
GO
Isoform 3 (identifier: P54219-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     307-338: Missing.

Note: No experimental confirmation available.
Show »
Length:493
Mass (Da):52,677
Checksum:i95F336015020D3B5
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_0218564T → P.1 PublicationCorresponds to variant rs2270641dbSNPEnsembl.1
Natural variantiVAR_05200211R → Q.Corresponds to variant rs17092144dbSNPEnsembl.1
Natural variantiVAR_02463274A → V.Corresponds to variant rs17215815dbSNPEnsembl.1
Natural variantiVAR_02914982F → C.Corresponds to variant rs17215822dbSNPEnsembl.1
Natural variantiVAR_02463384F → S.Corresponds to variant rs17215801dbSNPEnsembl.1
Natural variantiVAR_02003398S → T.Corresponds to variant rs2270637dbSNPEnsembl.1
Natural variantiVAR_024634101A → P.Corresponds to variant rs17222218dbSNPEnsembl.1
Natural variantiVAR_012065136I → T.1 PublicationCorresponds to variant rs1390938dbSNPEnsembl.1
Natural variantiVAR_029150140G → R.Corresponds to variant rs17215808dbSNPEnsembl.1
Natural variantiVAR_024635164I → M.Corresponds to variant rs17222092dbSNPEnsembl.1
Natural variantiVAR_029151202I → T.Corresponds to variant rs17222120dbSNPEnsembl.1
Natural variantiVAR_024636249V → I.Corresponds to variant rs17215759dbSNPEnsembl.1
Natural variantiVAR_024637392L → V.Corresponds to variant rs17092104dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_046304307 – 338Missing in isoform 3. 1 PublicationAdd BLAST32
Alternative sequenceiVSP_046305445 – 525PSTGG…PDHEE → YSESGLPHGDPDVCNPEAHE GISSGGGQ in isoform 2. 1 PublicationAdd BLAST81

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U39905 mRNA. Translation: AAC50472.1.
AC025853 Genomic DNA. No translation available.
BC006317 mRNA. Translation: AAH06317.1.
CCDSiCCDS47814.1. [P54219-3]
CCDS47815.1. [P54219-2]
CCDS6013.1. [P54219-1]
RefSeqiNP_001129163.1. NM_001135691.2. [P54219-1]
NP_001135796.1. NM_001142324.1. [P54219-3]
NP_001135797.1. NM_001142325.1. [P54219-2]
NP_003044.1. NM_003053.3. [P54219-1]
XP_011542928.1. XM_011544626.1. [P54219-3]
XP_011542929.1. XM_011544627.1. [P54219-2]
UniGeneiHs.158322.

Genome annotation databases

EnsembliENST00000265808; ENSP00000265808; ENSG00000036565. [P54219-3]
ENST00000276373; ENSP00000276373; ENSG00000036565. [P54219-1]
ENST00000381608; ENSP00000371021; ENSG00000036565. [P54219-2]
ENST00000437980; ENSP00000413361; ENSG00000036565. [P54219-2]
ENST00000440926; ENSP00000387549; ENSG00000036565. [P54219-1]
ENST00000519026; ENSP00000429664; ENSG00000036565. [P54219-3]
GeneIDi6570.
KEGGihsa:6570.
UCSCiuc003wzm.3. human. [P54219-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U39905 mRNA. Translation: AAC50472.1.
AC025853 Genomic DNA. No translation available.
BC006317 mRNA. Translation: AAH06317.1.
CCDSiCCDS47814.1. [P54219-3]
CCDS47815.1. [P54219-2]
CCDS6013.1. [P54219-1]
RefSeqiNP_001129163.1. NM_001135691.2. [P54219-1]
NP_001135796.1. NM_001142324.1. [P54219-3]
NP_001135797.1. NM_001142325.1. [P54219-2]
NP_003044.1. NM_003053.3. [P54219-1]
XP_011542928.1. XM_011544626.1. [P54219-3]
XP_011542929.1. XM_011544627.1. [P54219-2]
UniGeneiHs.158322.

3D structure databases

ProteinModelPortaliP54219.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi112458. 56 interactors.
STRINGi9606.ENSP00000276373.

Chemistry databases

BindingDBiP54219.
ChEMBLiCHEMBL1838.
DrugBankiDB01363. Ephedra.
DB01577. Methamphetamine.
DB00368. Norepinephrine.
DB00206. Reserpine.
GuidetoPHARMACOLOGYi1011.

Protein family/group databases

TCDBi2.A.1.2.12. the major facilitator superfamily (mfs).

PTM databases

iPTMnetiP54219.
PhosphoSitePlusiP54219.

Polymorphism and mutation databases

BioMutaiSLC18A1.
DMDMi1722741.

Proteomic databases

PaxDbiP54219.
PeptideAtlasiP54219.
PRIDEiP54219.

Protocols and materials databases

DNASUi6570.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000265808; ENSP00000265808; ENSG00000036565. [P54219-3]
ENST00000276373; ENSP00000276373; ENSG00000036565. [P54219-1]
ENST00000381608; ENSP00000371021; ENSG00000036565. [P54219-2]
ENST00000437980; ENSP00000413361; ENSG00000036565. [P54219-2]
ENST00000440926; ENSP00000387549; ENSG00000036565. [P54219-1]
ENST00000519026; ENSP00000429664; ENSG00000036565. [P54219-3]
GeneIDi6570.
KEGGihsa:6570.
UCSCiuc003wzm.3. human. [P54219-1]

Organism-specific databases

CTDi6570.
DisGeNETi6570.
GeneCardsiSLC18A1.
HGNCiHGNC:10934. SLC18A1.
MIMi193002. gene.
neXtProtiNX_P54219.
OpenTargetsiENSG00000036565.
PharmGKBiPA324.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3764. Eukaryota.
ENOG410XW69. LUCA.
GeneTreeiENSGT00390000003851.
HOGENOMiHOG000018545.
HOVERGENiHBG055082.
InParanoidiP54219.
KOiK08155.
OMAiNNCLQGT.
OrthoDBiEOG091G0534.
PhylomeDBiP54219.
TreeFamiTF313494.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000036565-MONOMER.
ReactomeiR-HSA-442660. Na+/Cl- dependent neurotransmitter transporters.

Miscellaneous databases

GenomeRNAii6570.
PROiP54219.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000036565.
CleanExiHS_SLC18A1.
HS_VAT1.
ExpressionAtlasiP54219. baseline and differential.
GenevisibleiP54219. HS.

Family and domain databases

CDDicd06174. MFS. 1 hit.
InterProiIPR011701. MFS.
IPR020846. MFS_dom.
IPR004734. Multidrug-R.
[Graphical view]
PfamiPF07690. MFS_1. 1 hit.
[Graphical view]
SUPFAMiSSF103473. SSF103473. 2 hits.
TIGRFAMsiTIGR00880. 2_A_01_02. 1 hit.
PROSITEiPS50850. MFS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiVMAT1_HUMAN
AccessioniPrimary (citable) accession number: P54219
Secondary accession number(s): E9PDJ5, Q9BRE4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: November 2, 2016
This is version 146 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 8
    Human chromosome 8: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.