Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P54219

- VMAT1_HUMAN

UniProt

P54219 - VMAT1_HUMAN

Protein

Chromaffin granule amine transporter

Gene

SLC18A1

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 130 (01 Oct 2014)
      Sequence version 1 (01 Oct 1996)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Involved in the transport of biogenic monoamines, such as serotonin, from the cytoplasm into the secretory vesicles of neuroendocrine and endocrine cells.1 Publication

    GO - Molecular functioni

    1. drug transmembrane transporter activity Source: InterPro
    2. monoamine transmembrane transporter activity Source: UniProtKB

    GO - Biological processi

    1. monoamine transport Source: UniProtKB
    2. neurotransmitter transport Source: UniProtKB-KW
    3. transmembrane transport Source: Reactome

    Keywords - Biological processi

    Neurotransmitter transport, Transport

    Enzyme and pathway databases

    ReactomeiREACT_20506. Na+/Cl- dependent neurotransmitter transporters.

    Protein family/group databases

    TCDBi2.A.1.2.12. the major facilitator superfamily (mfs).

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Chromaffin granule amine transporter
    Alternative name(s):
    Solute carrier family 18 member 1
    Vesicular amine transporter 1
    Short name:
    VAT1
    Gene namesi
    Name:SLC18A1
    Synonyms:VAT1, VMAT1
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 8

    Organism-specific databases

    HGNCiHGNC:10934. SLC18A1.

    Subcellular locationi

    GO - Cellular componenti

    1. clathrin-sculpted monoamine transport vesicle membrane Source: Reactome
    2. endoplasmic reticulum membrane Source: UniProtKB-SubCell
    3. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Cytoplasmic vesicle, Endoplasmic reticulum, Membrane

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA324.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 525525Chromaffin granule amine transporterPRO_0000127510Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi58 – 581N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi87 – 871N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi104 – 1041N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    PaxDbiP54219.
    PRIDEiP54219.

    PTM databases

    PhosphoSiteiP54219.

    Expressioni

    Gene expression databases

    ArrayExpressiP54219.
    BgeeiP54219.
    CleanExiHS_SLC18A1.
    HS_VAT1.
    GenevestigatoriP54219.

    Organism-specific databases

    HPAiHPA007210.

    Interactioni

    Protein-protein interaction databases

    BioGridi112458. 2 interactions.
    STRINGi9606.ENSP00000276373.

    Structurei

    3D structure databases

    ProteinModelPortaliP54219.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 2121CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini43 – 13896Lumenal, vesicleSequence AnalysisAdd
    BLAST
    Topological domaini159 – 1679CytoplasmicSequence Analysis
    Topological domaini189 – 1979Lumenal, vesicleSequence Analysis
    Topological domaini219 – 2279CytoplasmicSequence Analysis
    Topological domaini251 – 2566Lumenal, vesicleSequence Analysis
    Topological domaini280 – 29920CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini320 – 33516Lumenal, vesicleSequence AnalysisAdd
    BLAST
    Topological domaini361 – 3655CytoplasmicSequence Analysis
    Topological domaini387 – 39711Lumenal, vesicleSequence AnalysisAdd
    BLAST
    Topological domaini419 – 4224CytoplasmicSequence Analysis
    Topological domaini444 – 4485Lumenal, vesicleSequence Analysis
    Topological domaini471 – 52555CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei22 – 4221HelicalSequence AnalysisAdd
    BLAST
    Transmembranei139 – 15820HelicalSequence AnalysisAdd
    BLAST
    Transmembranei168 – 18821HelicalSequence AnalysisAdd
    BLAST
    Transmembranei198 – 21821HelicalSequence AnalysisAdd
    BLAST
    Transmembranei228 – 25023HelicalSequence AnalysisAdd
    BLAST
    Transmembranei257 – 27923HelicalSequence AnalysisAdd
    BLAST
    Transmembranei300 – 31920HelicalSequence AnalysisAdd
    BLAST
    Transmembranei336 – 36025HelicalSequence AnalysisAdd
    BLAST
    Transmembranei366 – 38621HelicalSequence AnalysisAdd
    BLAST
    Transmembranei398 – 41821HelicalSequence AnalysisAdd
    BLAST
    Transmembranei423 – 44321HelicalSequence AnalysisAdd
    BLAST
    Transmembranei449 – 47022HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG255549.
    HOGENOMiHOG000018545.
    HOVERGENiHBG055082.
    InParanoidiP54219.
    KOiK08155.
    OMAiNNCLQGT.
    OrthoDBiEOG79CXZ7.
    PhylomeDBiP54219.
    TreeFamiTF313494.

    Family and domain databases

    InterProiIPR011701. MFS.
    IPR020846. MFS_dom.
    IPR016196. MFS_dom_general_subst_transpt.
    IPR004734. Multidrug-R.
    [Graphical view]
    PfamiPF07690. MFS_1. 1 hit.
    [Graphical view]
    SUPFAMiSSF103473. SSF103473. 2 hits.
    TIGRFAMsiTIGR00880. 2_A_01_02. 1 hit.
    PROSITEiPS50850. MFS. 1 hit.
    [Graphical view]

    Sequences (3)i

    Sequence statusi: Complete.

    This entry describes 3 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: P54219-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MLRTILDAPQ RLLKEGRASR QLVLVVVFVA LLLDNMLFTV VVPIVPTFLY    50
    DMEFKEVNSS LHLGHAGSSP HALASPAFST IFSFFNNNTV AVEESVPSGI 100
    AWMNDTASTI PPPATEAISA HKNNCLQGTG FLEEEITRVG VLFASKAVMQ 150
    LLVNPFVGPL TNRIGYHIPM FAGFVIMFLS TVMFAFSGTY TLLFVARTLQ 200
    GIGSSFSSVA GLGMLASVYT DDHERGRAMG TALGGLALGL LVGAPFGSVM 250
    YEFVGKSAPF LILAFLALLD GALQLCILQP SKVSPESAKG TPLFMLLKDP 300
    YILVAAGSIC FANMGVAILE PTLPIWMMQT MCSPKWQLGL AFLPASVSYL 350
    IGTNLFGVLA NKMGRWLCSL IGMLVVGTSL LCVPLAHNIF GLIGPNAGLG 400
    LAIGMVDSSM MPIMGHLVDL RHTSVYGSVY AIADVAFCMG FAIGPSTGGA 450
    IVKAIGFPWL MVITGVINIV YAPLCYYLRS PPAKEEKLAI LSQDCPMETR 500
    MYATQKPTKE FPLGEDSDEE PDHEE 525
    Length:525
    Mass (Da):56,257
    Last modified:October 1, 1996 - v1
    Checksum:i041F2959F4F2BFBC
    GO
    Isoform 2 (identifier: P54219-2) [UniParc]FASTAAdd to Basket

    Also known as: VMAT1delta15

    The sequence of this isoform differs from the canonical sequence as follows:
         445-525: PSTGGAIVKA...DSDEEPDHEE → YSESGLPHGDPDVCNPEAHEGISSGGGQ

    Note: Unable to uptake serotonin.

    Show »
    Length:472
    Mass (Da):50,026
    Checksum:iAC98F90178CFEDC1
    GO
    Isoform 3 (identifier: P54219-3) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         307-338: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:493
    Mass (Da):52,677
    Checksum:i95F336015020D3B5
    GO

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti4 – 41T → P.1 Publication
    Corresponds to variant rs2270641 [ dbSNP | Ensembl ].
    VAR_021856
    Natural varianti11 – 111R → Q.
    Corresponds to variant rs17092144 [ dbSNP | Ensembl ].
    VAR_052002
    Natural varianti74 – 741A → V.
    Corresponds to variant rs17215815 [ dbSNP | Ensembl ].
    VAR_024632
    Natural varianti82 – 821F → C.
    Corresponds to variant rs17215822 [ dbSNP | Ensembl ].
    VAR_029149
    Natural varianti84 – 841F → S.
    Corresponds to variant rs17215801 [ dbSNP | Ensembl ].
    VAR_024633
    Natural varianti98 – 981S → T.
    Corresponds to variant rs2270637 [ dbSNP | Ensembl ].
    VAR_020033
    Natural varianti101 – 1011A → P.
    Corresponds to variant rs17222218 [ dbSNP | Ensembl ].
    VAR_024634
    Natural varianti136 – 1361I → T.1 Publication
    Corresponds to variant rs1390938 [ dbSNP | Ensembl ].
    VAR_012065
    Natural varianti140 – 1401G → R.
    Corresponds to variant rs17215808 [ dbSNP | Ensembl ].
    VAR_029150
    Natural varianti164 – 1641I → M.
    Corresponds to variant rs17222092 [ dbSNP | Ensembl ].
    VAR_024635
    Natural varianti202 – 2021I → T.
    Corresponds to variant rs17222120 [ dbSNP | Ensembl ].
    VAR_029151
    Natural varianti249 – 2491V → I.
    Corresponds to variant rs17215759 [ dbSNP | Ensembl ].
    VAR_024636
    Natural varianti392 – 3921L → V.
    Corresponds to variant rs17092104 [ dbSNP | Ensembl ].
    VAR_024637

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei307 – 33832Missing in isoform 3. 1 PublicationVSP_046304Add
    BLAST
    Alternative sequencei445 – 52581PSTGG…PDHEE → YSESGLPHGDPDVCNPEAHE GISSGGGQ in isoform 2. 1 PublicationVSP_046305Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U39905 mRNA. Translation: AAC50472.1.
    AC025853 Genomic DNA. No translation available.
    BC006317 mRNA. Translation: AAH06317.1.
    CCDSiCCDS47814.1. [P54219-3]
    CCDS47815.1. [P54219-2]
    CCDS6013.1. [P54219-1]
    RefSeqiNP_001129163.1. NM_001135691.2. [P54219-1]
    NP_001135796.1. NM_001142324.1. [P54219-3]
    NP_001135797.1. NM_001142325.1. [P54219-2]
    NP_003044.1. NM_003053.3. [P54219-1]
    UniGeneiHs.158322.

    Genome annotation databases

    EnsembliENST00000265808; ENSP00000265808; ENSG00000036565. [P54219-3]
    ENST00000276373; ENSP00000276373; ENSG00000036565. [P54219-1]
    ENST00000381608; ENSP00000371021; ENSG00000036565. [P54219-2]
    ENST00000437980; ENSP00000413361; ENSG00000036565. [P54219-2]
    ENST00000440926; ENSP00000387549; ENSG00000036565. [P54219-1]
    ENST00000519026; ENSP00000429664; ENSG00000036565. [P54219-3]
    GeneIDi6570.
    KEGGihsa:6570.
    UCSCiuc003wzm.3. human. [P54219-1]
    uc003wzn.3. human.

    Polymorphism databases

    DMDMi1722741.

    Keywords - Coding sequence diversityi

    Alternative splicing, Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U39905 mRNA. Translation: AAC50472.1 .
    AC025853 Genomic DNA. No translation available.
    BC006317 mRNA. Translation: AAH06317.1 .
    CCDSi CCDS47814.1. [P54219-3 ]
    CCDS47815.1. [P54219-2 ]
    CCDS6013.1. [P54219-1 ]
    RefSeqi NP_001129163.1. NM_001135691.2. [P54219-1 ]
    NP_001135796.1. NM_001142324.1. [P54219-3 ]
    NP_001135797.1. NM_001142325.1. [P54219-2 ]
    NP_003044.1. NM_003053.3. [P54219-1 ]
    UniGenei Hs.158322.

    3D structure databases

    ProteinModelPortali P54219.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 112458. 2 interactions.
    STRINGi 9606.ENSP00000276373.

    Chemistry

    BindingDBi P54219.
    ChEMBLi CHEMBL1838.
    GuidetoPHARMACOLOGYi 1011.

    Protein family/group databases

    TCDBi 2.A.1.2.12. the major facilitator superfamily (mfs).

    PTM databases

    PhosphoSitei P54219.

    Polymorphism databases

    DMDMi 1722741.

    Proteomic databases

    PaxDbi P54219.
    PRIDEi P54219.

    Protocols and materials databases

    DNASUi 6570.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000265808 ; ENSP00000265808 ; ENSG00000036565 . [P54219-3 ]
    ENST00000276373 ; ENSP00000276373 ; ENSG00000036565 . [P54219-1 ]
    ENST00000381608 ; ENSP00000371021 ; ENSG00000036565 . [P54219-2 ]
    ENST00000437980 ; ENSP00000413361 ; ENSG00000036565 . [P54219-2 ]
    ENST00000440926 ; ENSP00000387549 ; ENSG00000036565 . [P54219-1 ]
    ENST00000519026 ; ENSP00000429664 ; ENSG00000036565 . [P54219-3 ]
    GeneIDi 6570.
    KEGGi hsa:6570.
    UCSCi uc003wzm.3. human. [P54219-1 ]
    uc003wzn.3. human.

    Organism-specific databases

    CTDi 6570.
    GeneCardsi GC08M020046.
    HGNCi HGNC:10934. SLC18A1.
    HPAi HPA007210.
    MIMi 193002. gene.
    neXtProti NX_P54219.
    PharmGKBi PA324.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG255549.
    HOGENOMi HOG000018545.
    HOVERGENi HBG055082.
    InParanoidi P54219.
    KOi K08155.
    OMAi NNCLQGT.
    OrthoDBi EOG79CXZ7.
    PhylomeDBi P54219.
    TreeFami TF313494.

    Enzyme and pathway databases

    Reactomei REACT_20506. Na+/Cl- dependent neurotransmitter transporters.

    Miscellaneous databases

    GenomeRNAii 6570.
    NextBioi 25563.
    PROi P54219.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P54219.
    Bgeei P54219.
    CleanExi HS_SLC18A1.
    HS_VAT1.
    Genevestigatori P54219.

    Family and domain databases

    InterProi IPR011701. MFS.
    IPR020846. MFS_dom.
    IPR016196. MFS_dom_general_subst_transpt.
    IPR004734. Multidrug-R.
    [Graphical view ]
    Pfami PF07690. MFS_1. 1 hit.
    [Graphical view ]
    SUPFAMi SSF103473. SSF103473. 2 hits.
    TIGRFAMsi TIGR00880. 2_A_01_02. 1 hit.
    PROSITEi PS50850. MFS. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Distinct pharmacological properties and distribution in neurons and endocrine cells of two isoforms of the human vesicular monoamine transporter."
      Erickson J.D., Schaefer M.K.-H., Bonner T.I., Eiden L.E., Weihe E.
      Proc. Natl. Acad. Sci. U.S.A. 93:5166-5171(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
      Tissue: Pheochromocytoma.
    2. "DNA sequence and analysis of human chromosome 8."
      Nusbaum C., Mikkelsen T.S., Zody M.C., Asakawa S., Taudien S., Garber M., Kodira C.D., Schueler M.G., Shimizu A., Whittaker C.A., Chang J.L., Cuomo C.A., Dewar K., FitzGerald M.G., Yang X., Allen N.R., Anderson S., Asakawa T.
      , Blechschmidt K., Bloom T., Borowsky M.L., Butler J., Cook A., Corum B., DeArellano K., DeCaprio D., Dooley K.T., Dorris L. III, Engels R., Gloeckner G., Hafez N., Hagopian D.S., Hall J.L., Ishikawa S.K., Jaffe D.B., Kamat A., Kudoh J., Lehmann R., Lokitsang T., Macdonald P., Major J.E., Matthews C.D., Mauceli E., Menzel U., Mihalev A.H., Minoshima S., Murayama Y., Naylor J.W., Nicol R., Nguyen C., O'Leary S.B., O'Neill K., Parker S.C.J., Polley A., Raymond C.K., Reichwald K., Rodriguez J., Sasaki T., Schilhabel M., Siddiqui R., Smith C.L., Sneddon T.P., Talamas J.A., Tenzin P., Topham K., Venkataraman V., Wen G., Yamazaki S., Young S.K., Zeng Q., Zimmer A.R., Rosenthal A., Birren B.W., Platzer M., Shimizu N., Lander E.S.
      Nature 439:331-335(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3), VARIANTS PRO-4 AND THR-136.
      Tissue: Brain.
    4. "Identification and characterization of a novel splicing variant of vesicular monoamine transporter 1."
      Essand M., Vikman S., Grawe J., Gedda L., Hellberg C., Oberg K., Totterman T.H., Giandomenico V.
      J. Mol. Endocrinol. 35:489-501(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 445-472 (ISOFORM 2), FUNCTION, ALTERNATIVE SPLICING, SUBCELLULAR LOCATION.

    Entry informationi

    Entry nameiVMAT1_HUMAN
    AccessioniPrimary (citable) accession number: P54219
    Secondary accession number(s): E9PDJ5, Q9BRE4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 130 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 8
      Human chromosome 8: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3