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P54219

- VMAT1_HUMAN

UniProt

P54219 - VMAT1_HUMAN

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Protein
Chromaffin granule amine transporter
Gene
SLC18A1, VAT1, VMAT1
Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at transcript leveli

Functioni

Involved in the transport of biogenic monoamines, such as serotonin, from the cytoplasm into the secretory vesicles of neuroendocrine and endocrine cells.1 Publication

GO - Molecular functioni

  1. drug transmembrane transporter activity Source: InterPro
  2. monoamine transmembrane transporter activity Source: UniProtKB
Complete GO annotation...

GO - Biological processi

  1. monoamine transport Source: UniProtKB
  2. neurotransmitter transport Source: UniProtKB-KW
  3. transmembrane transport Source: Reactome
Complete GO annotation...

Keywords - Biological processi

Neurotransmitter transport, Transport

Enzyme and pathway databases

ReactomeiREACT_20506. Na+/Cl- dependent neurotransmitter transporters.

Protein family/group databases

TCDBi2.A.1.2.12. the major facilitator superfamily (mfs).

Names & Taxonomyi

Protein namesi
Recommended name:
Chromaffin granule amine transporter
Alternative name(s):
Solute carrier family 18 member 1
Vesicular amine transporter 1
Short name:
VAT1
Gene namesi
Name:SLC18A1
Synonyms:VAT1, VMAT1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 8

Organism-specific databases

HGNCiHGNC:10934. SLC18A1.

Subcellular locationi

Isoform 1 : Cytoplasmic vesicle membrane; Multi-pass membrane protein 1 Publication
Isoform 2 : Endoplasmic reticulum membrane; Multi-pass membrane protein 1 Publication

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 2121Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei22 – 4221Helical; Reviewed prediction
Add
BLAST
Topological domaini43 – 13896Lumenal, vesicle Reviewed prediction
Add
BLAST
Transmembranei139 – 15820Helical; Reviewed prediction
Add
BLAST
Topological domaini159 – 1679Cytoplasmic Reviewed prediction
Transmembranei168 – 18821Helical; Reviewed prediction
Add
BLAST
Topological domaini189 – 1979Lumenal, vesicle Reviewed prediction
Transmembranei198 – 21821Helical; Reviewed prediction
Add
BLAST
Topological domaini219 – 2279Cytoplasmic Reviewed prediction
Transmembranei228 – 25023Helical; Reviewed prediction
Add
BLAST
Topological domaini251 – 2566Lumenal, vesicle Reviewed prediction
Transmembranei257 – 27923Helical; Reviewed prediction
Add
BLAST
Topological domaini280 – 29920Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei300 – 31920Helical; Reviewed prediction
Add
BLAST
Topological domaini320 – 33516Lumenal, vesicle Reviewed prediction
Add
BLAST
Transmembranei336 – 36025Helical; Reviewed prediction
Add
BLAST
Topological domaini361 – 3655Cytoplasmic Reviewed prediction
Transmembranei366 – 38621Helical; Reviewed prediction
Add
BLAST
Topological domaini387 – 39711Lumenal, vesicle Reviewed prediction
Add
BLAST
Transmembranei398 – 41821Helical; Reviewed prediction
Add
BLAST
Topological domaini419 – 4224Cytoplasmic Reviewed prediction
Transmembranei423 – 44321Helical; Reviewed prediction
Add
BLAST
Topological domaini444 – 4485Lumenal, vesicle Reviewed prediction
Transmembranei449 – 47022Helical; Reviewed prediction
Add
BLAST
Topological domaini471 – 52555Cytoplasmic Reviewed prediction
Add
BLAST

GO - Cellular componenti

  1. clathrin-sculpted monoamine transport vesicle membrane Source: Reactome
  2. endoplasmic reticulum membrane Source: UniProtKB-SubCell
  3. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasmic vesicle, Endoplasmic reticulum, Membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA324.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 525525Chromaffin granule amine transporter
PRO_0000127510Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi58 – 581N-linked (GlcNAc...) Reviewed prediction
Glycosylationi87 – 871N-linked (GlcNAc...) Reviewed prediction
Glycosylationi104 – 1041N-linked (GlcNAc...) Reviewed prediction

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiP54219.
PRIDEiP54219.

PTM databases

PhosphoSiteiP54219.

Expressioni

Gene expression databases

ArrayExpressiP54219.
BgeeiP54219.
CleanExiHS_SLC18A1.
HS_VAT1.
GenevestigatoriP54219.

Organism-specific databases

HPAiHPA007210.

Interactioni

Protein-protein interaction databases

BioGridi112458. 2 interactions.
STRINGi9606.ENSP00000276373.

Structurei

3D structure databases

ProteinModelPortaliP54219.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG255549.
HOGENOMiHOG000018545.
HOVERGENiHBG055082.
InParanoidiP54219.
KOiK08155.
OMAiNNCLQGT.
OrthoDBiEOG79CXZ7.
PhylomeDBiP54219.
TreeFamiTF313494.

Family and domain databases

InterProiIPR011701. MFS.
IPR020846. MFS_dom.
IPR016196. MFS_dom_general_subst_transpt.
IPR004734. Multidrug-R.
[Graphical view]
PfamiPF07690. MFS_1. 1 hit.
[Graphical view]
SUPFAMiSSF103473. SSF103473. 2 hits.
TIGRFAMsiTIGR00880. 2_A_01_02. 1 hit.
PROSITEiPS50850. MFS. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: P54219-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MLRTILDAPQ RLLKEGRASR QLVLVVVFVA LLLDNMLFTV VVPIVPTFLY    50
DMEFKEVNSS LHLGHAGSSP HALASPAFST IFSFFNNNTV AVEESVPSGI 100
AWMNDTASTI PPPATEAISA HKNNCLQGTG FLEEEITRVG VLFASKAVMQ 150
LLVNPFVGPL TNRIGYHIPM FAGFVIMFLS TVMFAFSGTY TLLFVARTLQ 200
GIGSSFSSVA GLGMLASVYT DDHERGRAMG TALGGLALGL LVGAPFGSVM 250
YEFVGKSAPF LILAFLALLD GALQLCILQP SKVSPESAKG TPLFMLLKDP 300
YILVAAGSIC FANMGVAILE PTLPIWMMQT MCSPKWQLGL AFLPASVSYL 350
IGTNLFGVLA NKMGRWLCSL IGMLVVGTSL LCVPLAHNIF GLIGPNAGLG 400
LAIGMVDSSM MPIMGHLVDL RHTSVYGSVY AIADVAFCMG FAIGPSTGGA 450
IVKAIGFPWL MVITGVINIV YAPLCYYLRS PPAKEEKLAI LSQDCPMETR 500
MYATQKPTKE FPLGEDSDEE PDHEE 525
Length:525
Mass (Da):56,257
Last modified:October 1, 1996 - v1
Checksum:i041F2959F4F2BFBC
GO
Isoform 2 (identifier: P54219-2) [UniParc]FASTAAdd to Basket

Also known as: VMAT1delta15

The sequence of this isoform differs from the canonical sequence as follows:
     445-525: PSTGGAIVKA...DSDEEPDHEE → YSESGLPHGDPDVCNPEAHEGISSGGGQ

Note: Unable to uptake serotonin.

Show »
Length:472
Mass (Da):50,026
Checksum:iAC98F90178CFEDC1
GO
Isoform 3 (identifier: P54219-3) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     307-338: Missing.

Note: No experimental confirmation available.

Show »
Length:493
Mass (Da):52,677
Checksum:i95F336015020D3B5
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti4 – 41T → P.1 Publication
Corresponds to variant rs2270641 [ dbSNP | Ensembl ].
VAR_021856
Natural varianti11 – 111R → Q.
Corresponds to variant rs17092144 [ dbSNP | Ensembl ].
VAR_052002
Natural varianti74 – 741A → V.
Corresponds to variant rs17215815 [ dbSNP | Ensembl ].
VAR_024632
Natural varianti82 – 821F → C.
Corresponds to variant rs17215822 [ dbSNP | Ensembl ].
VAR_029149
Natural varianti84 – 841F → S.
Corresponds to variant rs17215801 [ dbSNP | Ensembl ].
VAR_024633
Natural varianti98 – 981S → T.
Corresponds to variant rs2270637 [ dbSNP | Ensembl ].
VAR_020033
Natural varianti101 – 1011A → P.
Corresponds to variant rs17222218 [ dbSNP | Ensembl ].
VAR_024634
Natural varianti136 – 1361I → T.1 Publication
Corresponds to variant rs1390938 [ dbSNP | Ensembl ].
VAR_012065
Natural varianti140 – 1401G → R.
Corresponds to variant rs17215808 [ dbSNP | Ensembl ].
VAR_029150
Natural varianti164 – 1641I → M.
Corresponds to variant rs17222092 [ dbSNP | Ensembl ].
VAR_024635
Natural varianti202 – 2021I → T.
Corresponds to variant rs17222120 [ dbSNP | Ensembl ].
VAR_029151
Natural varianti249 – 2491V → I.
Corresponds to variant rs17215759 [ dbSNP | Ensembl ].
VAR_024636
Natural varianti392 – 3921L → V.
Corresponds to variant rs17092104 [ dbSNP | Ensembl ].
VAR_024637

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei307 – 33832Missing in isoform 3.
VSP_046304Add
BLAST
Alternative sequencei445 – 52581PSTGG…PDHEE → YSESGLPHGDPDVCNPEAHE GISSGGGQ in isoform 2.
VSP_046305Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U39905 mRNA. Translation: AAC50472.1.
AC025853 Genomic DNA. No translation available.
BC006317 mRNA. Translation: AAH06317.1.
CCDSiCCDS47814.1. [P54219-3]
CCDS47815.1. [P54219-2]
CCDS6013.1. [P54219-1]
RefSeqiNP_001129163.1. NM_001135691.2. [P54219-1]
NP_001135796.1. NM_001142324.1. [P54219-3]
NP_001135797.1. NM_001142325.1. [P54219-2]
NP_003044.1. NM_003053.3. [P54219-1]
UniGeneiHs.158322.

Genome annotation databases

EnsembliENST00000265808; ENSP00000265808; ENSG00000036565. [P54219-3]
ENST00000276373; ENSP00000276373; ENSG00000036565. [P54219-1]
ENST00000381608; ENSP00000371021; ENSG00000036565. [P54219-2]
ENST00000437980; ENSP00000413361; ENSG00000036565. [P54219-2]
ENST00000440926; ENSP00000387549; ENSG00000036565. [P54219-1]
ENST00000519026; ENSP00000429664; ENSG00000036565. [P54219-3]
GeneIDi6570.
KEGGihsa:6570.
UCSCiuc003wzm.3. human. [P54219-1]
uc003wzn.3. human.

Polymorphism databases

DMDMi1722741.

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U39905 mRNA. Translation: AAC50472.1 .
AC025853 Genomic DNA. No translation available.
BC006317 mRNA. Translation: AAH06317.1 .
CCDSi CCDS47814.1. [P54219-3 ]
CCDS47815.1. [P54219-2 ]
CCDS6013.1. [P54219-1 ]
RefSeqi NP_001129163.1. NM_001135691.2. [P54219-1 ]
NP_001135796.1. NM_001142324.1. [P54219-3 ]
NP_001135797.1. NM_001142325.1. [P54219-2 ]
NP_003044.1. NM_003053.3. [P54219-1 ]
UniGenei Hs.158322.

3D structure databases

ProteinModelPortali P54219.
ModBasei Search...

Protein-protein interaction databases

BioGridi 112458. 2 interactions.
STRINGi 9606.ENSP00000276373.

Chemistry

BindingDBi P54219.
ChEMBLi CHEMBL1838.
GuidetoPHARMACOLOGYi 1011.

Protein family/group databases

TCDBi 2.A.1.2.12. the major facilitator superfamily (mfs).

PTM databases

PhosphoSitei P54219.

Polymorphism databases

DMDMi 1722741.

Proteomic databases

PaxDbi P54219.
PRIDEi P54219.

Protocols and materials databases

DNASUi 6570.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000265808 ; ENSP00000265808 ; ENSG00000036565 . [P54219-3 ]
ENST00000276373 ; ENSP00000276373 ; ENSG00000036565 . [P54219-1 ]
ENST00000381608 ; ENSP00000371021 ; ENSG00000036565 . [P54219-2 ]
ENST00000437980 ; ENSP00000413361 ; ENSG00000036565 . [P54219-2 ]
ENST00000440926 ; ENSP00000387549 ; ENSG00000036565 . [P54219-1 ]
ENST00000519026 ; ENSP00000429664 ; ENSG00000036565 . [P54219-3 ]
GeneIDi 6570.
KEGGi hsa:6570.
UCSCi uc003wzm.3. human. [P54219-1 ]
uc003wzn.3. human.

Organism-specific databases

CTDi 6570.
GeneCardsi GC08M020046.
HGNCi HGNC:10934. SLC18A1.
HPAi HPA007210.
MIMi 193002. gene.
neXtProti NX_P54219.
PharmGKBi PA324.
GenAtlasi Search...

Phylogenomic databases

eggNOGi NOG255549.
HOGENOMi HOG000018545.
HOVERGENi HBG055082.
InParanoidi P54219.
KOi K08155.
OMAi NNCLQGT.
OrthoDBi EOG79CXZ7.
PhylomeDBi P54219.
TreeFami TF313494.

Enzyme and pathway databases

Reactomei REACT_20506. Na+/Cl- dependent neurotransmitter transporters.

Miscellaneous databases

GenomeRNAii 6570.
NextBioi 25563.
PROi P54219.
SOURCEi Search...

Gene expression databases

ArrayExpressi P54219.
Bgeei P54219.
CleanExi HS_SLC18A1.
HS_VAT1.
Genevestigatori P54219.

Family and domain databases

InterProi IPR011701. MFS.
IPR020846. MFS_dom.
IPR016196. MFS_dom_general_subst_transpt.
IPR004734. Multidrug-R.
[Graphical view ]
Pfami PF07690. MFS_1. 1 hit.
[Graphical view ]
SUPFAMi SSF103473. SSF103473. 2 hits.
TIGRFAMsi TIGR00880. 2_A_01_02. 1 hit.
PROSITEi PS50850. MFS. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Distinct pharmacological properties and distribution in neurons and endocrine cells of two isoforms of the human vesicular monoamine transporter."
    Erickson J.D., Schaefer M.K.-H., Bonner T.I., Eiden L.E., Weihe E.
    Proc. Natl. Acad. Sci. U.S.A. 93:5166-5171(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    Tissue: Pheochromocytoma.
  2. "DNA sequence and analysis of human chromosome 8."
    Nusbaum C., Mikkelsen T.S., Zody M.C., Asakawa S., Taudien S., Garber M., Kodira C.D., Schueler M.G., Shimizu A., Whittaker C.A., Chang J.L., Cuomo C.A., Dewar K., FitzGerald M.G., Yang X., Allen N.R., Anderson S., Asakawa T.
    , Blechschmidt K., Bloom T., Borowsky M.L., Butler J., Cook A., Corum B., DeArellano K., DeCaprio D., Dooley K.T., Dorris L. III, Engels R., Gloeckner G., Hafez N., Hagopian D.S., Hall J.L., Ishikawa S.K., Jaffe D.B., Kamat A., Kudoh J., Lehmann R., Lokitsang T., Macdonald P., Major J.E., Matthews C.D., Mauceli E., Menzel U., Mihalev A.H., Minoshima S., Murayama Y., Naylor J.W., Nicol R., Nguyen C., O'Leary S.B., O'Neill K., Parker S.C.J., Polley A., Raymond C.K., Reichwald K., Rodriguez J., Sasaki T., Schilhabel M., Siddiqui R., Smith C.L., Sneddon T.P., Talamas J.A., Tenzin P., Topham K., Venkataraman V., Wen G., Yamazaki S., Young S.K., Zeng Q., Zimmer A.R., Rosenthal A., Birren B.W., Platzer M., Shimizu N., Lander E.S.
    Nature 439:331-335(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3), VARIANTS PRO-4 AND THR-136.
    Tissue: Brain.
  4. "Identification and characterization of a novel splicing variant of vesicular monoamine transporter 1."
    Essand M., Vikman S., Grawe J., Gedda L., Hellberg C., Oberg K., Totterman T.H., Giandomenico V.
    J. Mol. Endocrinol. 35:489-501(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 445-472 (ISOFORM 2), FUNCTION, ALTERNATIVE SPLICING, SUBCELLULAR LOCATION.

Entry informationi

Entry nameiVMAT1_HUMAN
AccessioniPrimary (citable) accession number: P54219
Secondary accession number(s): E9PDJ5, Q9BRE4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: September 3, 2014
This is version 129 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 8
    Human chromosome 8: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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