Reviewed, UniProtKB/Swiss-Prot P54216 (ALF1_CAEEL)

Names and origin

Protein names

Recommended name:
    Fructose-bisphosphate aldolase 1
    EC=4.1.2.13
Alternative name(s):
    Aldolase CE-1
      Short name=CE1

Gene names

ORF Names:

T05D4.1

Organism

Caenorhabditis elegans [Complete proteome]

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	366 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	D-fructose 1,6-bisphosphate = glycerone phosphate + D-glyceraldehyde 3-phosphate.
Pathway	Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 4/4.
Tissue specificity	Ubiquitous.
Developmental stage	All stages of development.
Sequence similarities	Belongs to the class I fructose-bisphosphate aldolase family.

Ontologies

Keywords
Biological process	Glycolysis
Ligand	Schiff base
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycolysis Ref.1 Non-traceable author statement. Source: UniProtKB
Molecular function	fructose-bisphosphate aldolase activity Ref.1 Inferred from direct assay. Source: UniProtKB identical protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
itself		2	EBI-327260,EBI-327260

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 366	366	Fructose-bisphosphate aldolase 1		PRO_0000216928
Sites
	Active site	189	1	Proton acceptor By similarity
	Active site	231	1	Schiff-base intermediate with dihydroxyacetone-P By similarity
	Binding site	56	1	Substrate By similarity
	Binding site	147	1	Substrate By similarity
	Site	366	1	Necessary for preference for fructose 1,6-bisphosphate over fructose 1-phosphate
Experimental info
	Sequence conflict	172 – 173	2	GS → A in CAB03291. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P54216-1 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 5B9F91D486FFAEB5	FASTA	366	39,240
	10 20 30 40 50 60 MASYSQFLTK AQEDELRSIA NAIVTPGKGI LAADESTGSM DKRLNSIGLE NTEENRRKYR 70 80 90 100 110 120 QLLFTAGADL NKYISGVIMF HETFYQKTDD GKPFTALLQE QGIIPGIKVD KGVVPMAGTI 130 140 150 160 170 180 GEGTTQGLDD LNARCAQYKK DGAQFAKWRC VHKISSTTPS VTALKEIASN LGSRYASICQ 190 200 210 220 230 240 QNGLVPIVEP EILPDGEHCL ARGQKITETV LSYVYHALNE HHVFLEGTLL KPNMVTSGQS 250 260 270 280 290 300 FTGEKPSNAD IGLATVTALQ RGVPSAVPGV VFLSGGQSEE DATLNLNAIN QVSGKKPWAL 310 320 330 340 350 360 TFSYGRALQA SCLAKWAGKD ENIAAAQEVL LHRAQVNSLA SVGKYTGDAS ADAAASQSLF VANHSY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Caenorhabditis elegans has two isozymic forms, CE-1 and CE-2, of fructose-1,6-bisphosphate aldolase which are encoded by different genes." Inoue T., Yatsuki H., Kusakabe T., Joh K., Hori K. Arch. Biochem. Biophys. 339:226-234(1997) [PubMed: 9056253] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Bristol N2.
[2]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed: 9851916] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bristol N2.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	D83738 mRNA. Translation: BAA12091.1. Z81115 Genomic DNA. Translation: CAB03291.1.
PIR	T24514.
RefSeq	NP_741281.1.
UniGene	Cel.6851
3D structure databases
HSSP	HSSP built from PDB template 1ADO based on UniProtKB P00883.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:25939N.
IntAct	P54216. 2 interactions.
STRING	P54216.
Genome annotation databases
Ensembl	T05D4.1.1; T05D4.1.1; T05D4.1; Caenorhabditis elegans. [Genome view] T05D4.1.2; T05D4.1.2; T05D4.1; Caenorhabditis elegans. [Genome view]
GeneID	176788.
KEGG	cel:T05D4.1.
UCSC	T05D4.1.1. c. elegans.
Organism-specific databases
WormBase	WBGene00011474. T05D4.1.
WormPep	T05D4.1. CE16341. [WorfDB]
Enzyme and pathway databases
BRENDA	4.1.2.13. 672.
Gene expression databases
ArrayExpress	P54216.
Family and domain databases
InterPro	IPR000741. Aldolase_I. IPR013785. Aldolase_TIM. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
PANTHER	PTHR11627. Aldolase_I. 1 hit.
Pfam	PF00274. Glycolytic. 1 hit. [Graphical view]
ProDom	PD001128. Aldolase_I. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS00158. ALDOLASE_CLASS_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ALF1_CAEEL

Accession

Primary (citable) accession number: P54216
Secondary accession number(s): O45747

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	November 3, 2009
This is version 64 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Caenorhabditis annotation project