Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P54201

- UBPA_DICDI

UniProt

P54201 - UBPA_DICDI

Protein

Ubiquitin carboxyl-terminal hydrolase A

Gene

ubpA

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 95 (01 Oct 2014)
      Sequence version 2 (04 Dec 2007)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Required for development but not growth.1 Publication

    Catalytic activityi

    Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei328 – 3281NucleophilePROSITE-ProRule annotation
    Active sitei797 – 7971Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri189 – 26375UBP-typePROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. cysteine-type peptidase activity Source: UniProtKB-KW
    2. omega peptidase activity Source: InterPro
    3. ubiquitinyl hydrolase activity Source: InterPro
    4. zinc ion binding Source: InterPro

    GO - Biological processi

    1. ubiquitin-dependent protein catabolic process Source: InterPro

    Keywords - Molecular functioni

    Hydrolase, Protease, Thiol protease

    Keywords - Biological processi

    Ubl conjugation pathway

    Keywords - Ligandi

    Metal-binding, Zinc

    Protein family/group databases

    MEROPSiC19.104.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ubiquitin carboxyl-terminal hydrolase A (EC:3.4.19.12)
    Alternative name(s):
    Deubiquitinating enzyme A
    Ubiquitin thioesterase A
    Ubiquitin-specific-processing protease A
    Gene namesi
    Name:ubpA
    Synonyms:usp5
    ORF Names:DDB_G0291239
    OrganismiDictyostelium discoideum (Slime mold)
    Taxonomic identifieri44689 [NCBI]
    Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
    ProteomesiUP000002195: Chromosome 6, UP000002195: Unassembled WGS sequence

    Organism-specific databases

    dictyBaseiDDB_G0291239. ubpA.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 837837Ubiquitin carboxyl-terminal hydrolase APRO_0000080686Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi44689.DDB_0231492.

    Structurei

    3D structure databases

    ProteinModelPortaliP54201.
    SMRiP54201. Positions 167-276.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini319 – 835517USPAdd
    BLAST
    Domaini628 – 66942UBA 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini700 – 74041UBA 2PROSITE-ProRule annotationAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi684 – 6907Poly-Asn

    Sequence similaritiesi

    Belongs to the peptidase C19 family.Curated
    Contains 2 UBA domains.PROSITE-ProRule annotation
    Contains 1 UBP-type zinc finger.PROSITE-ProRule annotation
    Contains 1 USP domain.Curated

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri189 – 26375UBP-typePROSITE-ProRule annotationAdd
    BLAST

    Keywords - Domaini

    Repeat, Zinc-finger

    Phylogenomic databases

    eggNOGiCOG5207.
    KOiK11836.
    PhylomeDBiP54201.

    Family and domain databases

    Gene3Di3.30.40.10. 1 hit.
    InterProiIPR018200. Pept_C19ubi-hydrolase_C_CS.
    IPR001394. Peptidase_C19_UCH.
    IPR009060. UBA-like.
    IPR015940. UBA/transl_elong_EF1B_N_euk.
    IPR000449. UBA/Ts_N.
    IPR016652. Ubiquitinyl_hydrolase.
    IPR028889. UCH/PAN2.
    IPR013083. Znf_RING/FYVE/PHD.
    IPR001607. Znf_UBP.
    [Graphical view]
    PfamiPF00627. UBA. 2 hits.
    PF00443. UCH. 1 hit.
    PF02148. zf-UBP. 1 hit.
    [Graphical view]
    PIRSFiPIRSF016308. UBP. 1 hit.
    SMARTiSM00165. UBA. 2 hits.
    SM00290. ZnF_UBP. 1 hit.
    [Graphical view]
    SUPFAMiSSF46934. SSF46934. 1 hit.
    PROSITEiPS50030. UBA. 2 hits.
    PS00972. USP_1. 1 hit.
    PS00973. USP_2. 1 hit.
    PS50235. USP_3. 1 hit.
    PS50271. ZF_UBP. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P54201-1 [UniParc]FASTAAdd to Basket

    « Hide

    MELFPELKNI KVPTEKSRLW KDECCYCFDT PENGEGLFID LIGLLAFSKK    50
    YVQLNHQKTH HHLYLNFKKV AIVNEKVKSP TIENGGEEKP PKKLAIGVEG 100
    GFNVEDEEIK YEEHYKLYIF PDDKFLELSD PIIPENVRVC CEKIKTLNSQ 150
    SRKEEIVSWN AESVFPSAFA ESIIQLDNNT KKIDPKGPWR CDIEGCDKVE 200
    NLWLNLTDGF IGCGRKYADG TGGNGHAQEH FNQTQYPISV KLGTITKDHA 250
    DVYSYPEDDM VSDPLLFQHL THWGLNPNVM VKTEKSMAEL ELDQNLNFEF 300
    GKIQEKGKLL ENVFGPGLTG IENLGNSCYM SSVIQMIFAI DSFQTRYLKD 350
    REASFKDITQ DPTQSFEIQM SKLAHGLLSG DYSIPLSKPS KNANEESEAA 400
    TQIGIAPKMF KSLIGASHAE FSTMKQQDAH EYLQYLLEYI ERAEHSRPSW 450
    IQQANPTRLF QFHNEDRIEC GSSGQVKYTR RLENILSVPV NLDDATNKQE 500
    VAQYEETLKQ QNGVRQKDQE EIRPIIPLVS CINGFVEPYR VEDFLSPATG 550
    VKTFSLNSSR MATFPEVLII HLKKYTYNAD YTPKKLNVFM DVPDIIDIDS 600
    LRGRGIKEGE VPLKEGTINT TTKVPEPSFN QEVLDTLLSM DFPLVRCKKA 650
    LLATGGKDAE LAMNWIFEHT EDPDIDIEQT PVNNNNNNNN SSNSNDKLFV 700
    FNSQDVDNII GMGFTDSQAK LALKNTKGNL ERAADWLFSH IDNLDELVAK 750
    DNASTSSINP SLIPQSTTSL QPVSDGVGKY ELLGFISHLG NNVTCGHYVC 800
    HIKKNNRWIK FNDRHVQLSE QPPKELGYIY FYKRQLN 837
    Length:837
    Mass (Da):94,966
    Last modified:December 4, 2007 - v2
    Checksum:i5E50A4E2CFE68BB4
    GO

    Sequence cautioni

    The sequence EAL61603.2 differs from that shown. Reason: Erroneous gene model prediction.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti180 – 1801T → N in AAC71068. (PubMed:9786928)Curated
    Sequence conflicti683 – 6831N → I in AAC71068. (PubMed:9786928)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U48271 mRNA. Translation: AAC71068.1.
    AAFI02000177 Genomic DNA. Translation: EAL61603.2. Sequence problems.
    RefSeqiXP_635198.2. XM_630106.2.

    Genome annotation databases

    GeneIDi8628143.
    KEGGiddi:DDB_G0291239.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U48271 mRNA. Translation: AAC71068.1 .
    AAFI02000177 Genomic DNA. Translation: EAL61603.2 . Sequence problems.
    RefSeqi XP_635198.2. XM_630106.2.

    3D structure databases

    ProteinModelPortali P54201.
    SMRi P54201. Positions 167-276.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 44689.DDB_0231492.

    Protein family/group databases

    MEROPSi C19.104.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 8628143.
    KEGGi ddi:DDB_G0291239.

    Organism-specific databases

    dictyBasei DDB_G0291239. ubpA.

    Phylogenomic databases

    eggNOGi COG5207.
    KOi K11836.
    PhylomeDBi P54201.

    Family and domain databases

    Gene3Di 3.30.40.10. 1 hit.
    InterProi IPR018200. Pept_C19ubi-hydrolase_C_CS.
    IPR001394. Peptidase_C19_UCH.
    IPR009060. UBA-like.
    IPR015940. UBA/transl_elong_EF1B_N_euk.
    IPR000449. UBA/Ts_N.
    IPR016652. Ubiquitinyl_hydrolase.
    IPR028889. UCH/PAN2.
    IPR013083. Znf_RING/FYVE/PHD.
    IPR001607. Znf_UBP.
    [Graphical view ]
    Pfami PF00627. UBA. 2 hits.
    PF00443. UCH. 1 hit.
    PF02148. zf-UBP. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF016308. UBP. 1 hit.
    SMARTi SM00165. UBA. 2 hits.
    SM00290. ZnF_UBP. 1 hit.
    [Graphical view ]
    SUPFAMi SSF46934. SSF46934. 1 hit.
    PROSITEi PS50030. UBA. 2 hits.
    PS00972. USP_1. 1 hit.
    PS00973. USP_2. 1 hit.
    PS50235. USP_3. 1 hit.
    PS50271. ZF_UBP. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "A deubiquitinating enzyme that disassembles free polyubiquitin chains is required for development but not growth in Dictyostelium."
      Lindsey D.F., Amerik A., Deery W.J., Bishop J.D., Hochstrasser M., Gomer R.H.
      J. Biol. Chem. 273:29178-29187(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION.
    2. "The genome of the social amoeba Dictyostelium discoideum."
      Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
      , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
      Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: AX4.

    Entry informationi

    Entry nameiUBPA_DICDI
    AccessioniPrimary (citable) accession number: P54201
    Secondary accession number(s): Q54EP2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: December 4, 2007
    Last modified: October 1, 2014
    This is version 95 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Dictyostelium discoideum
      Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
    2. Peptidase families
      Classification of peptidase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3