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P54201

- UBPA_DICDI

UniProt

P54201 - UBPA_DICDI

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Protein

Ubiquitin carboxyl-terminal hydrolase A

Gene

ubpA

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli

Functioni

Required for development but not growth.1 Publication

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei328 – 3281NucleophilePROSITE-ProRule annotation
Active sitei797 – 7971Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri189 – 26375UBP-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. cysteine-type peptidase activity Source: UniProtKB-KW
  2. omega peptidase activity Source: InterPro
  3. ubiquitinyl hydrolase activity Source: InterPro
  4. zinc ion binding Source: InterPro

GO - Biological processi

  1. ubiquitin-dependent protein catabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Biological processi

Ubl conjugation pathway

Keywords - Ligandi

Metal-binding, Zinc

Protein family/group databases

MEROPSiC19.104.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin carboxyl-terminal hydrolase A (EC:3.4.19.12)
Alternative name(s):
Deubiquitinating enzyme A
Ubiquitin thioesterase A
Ubiquitin-specific-processing protease A
Gene namesi
Name:ubpA
Synonyms:usp5
ORF Names:DDB_G0291239
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
ProteomesiUP000002195: Chromosome 6, UP000002195: Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0291239. ubpA.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 837837Ubiquitin carboxyl-terminal hydrolase APRO_0000080686Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi44689.DDB_0231492.

Structurei

3D structure databases

ProteinModelPortaliP54201.
SMRiP54201. Positions 167-276.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini319 – 835517USPAdd
BLAST
Domaini628 – 66942UBA 1PROSITE-ProRule annotationAdd
BLAST
Domaini700 – 74041UBA 2PROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi684 – 6907Poly-Asn

Sequence similaritiesi

Belongs to the peptidase C19 family.Curated
Contains 2 UBA domains.PROSITE-ProRule annotation
Contains 1 UBP-type zinc finger.PROSITE-ProRule annotation
Contains 1 USP domain.Curated

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri189 – 26375UBP-typePROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiCOG5207.
InParanoidiP54201.
KOiK11836.
PhylomeDBiP54201.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiIPR018200. Pept_C19ubi-hydrolase_C_CS.
IPR001394. Peptidase_C19_UCH.
IPR009060. UBA-like.
IPR015940. UBA/transl_elong_EF1B_N_euk.
IPR000449. UBA/Ts_N.
IPR016652. Ubiquitinyl_hydrolase.
IPR028889. UCH/PAN2.
IPR013083. Znf_RING/FYVE/PHD.
IPR001607. Znf_UBP.
[Graphical view]
PfamiPF00627. UBA. 2 hits.
PF00443. UCH. 1 hit.
PF02148. zf-UBP. 1 hit.
[Graphical view]
PIRSFiPIRSF016308. UBP. 1 hit.
SMARTiSM00165. UBA. 2 hits.
SM00290. ZnF_UBP. 1 hit.
[Graphical view]
SUPFAMiSSF46934. SSF46934. 1 hit.
PROSITEiPS50030. UBA. 2 hits.
PS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
PS50271. ZF_UBP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P54201-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MELFPELKNI KVPTEKSRLW KDECCYCFDT PENGEGLFID LIGLLAFSKK
60 70 80 90 100
YVQLNHQKTH HHLYLNFKKV AIVNEKVKSP TIENGGEEKP PKKLAIGVEG
110 120 130 140 150
GFNVEDEEIK YEEHYKLYIF PDDKFLELSD PIIPENVRVC CEKIKTLNSQ
160 170 180 190 200
SRKEEIVSWN AESVFPSAFA ESIIQLDNNT KKIDPKGPWR CDIEGCDKVE
210 220 230 240 250
NLWLNLTDGF IGCGRKYADG TGGNGHAQEH FNQTQYPISV KLGTITKDHA
260 270 280 290 300
DVYSYPEDDM VSDPLLFQHL THWGLNPNVM VKTEKSMAEL ELDQNLNFEF
310 320 330 340 350
GKIQEKGKLL ENVFGPGLTG IENLGNSCYM SSVIQMIFAI DSFQTRYLKD
360 370 380 390 400
REASFKDITQ DPTQSFEIQM SKLAHGLLSG DYSIPLSKPS KNANEESEAA
410 420 430 440 450
TQIGIAPKMF KSLIGASHAE FSTMKQQDAH EYLQYLLEYI ERAEHSRPSW
460 470 480 490 500
IQQANPTRLF QFHNEDRIEC GSSGQVKYTR RLENILSVPV NLDDATNKQE
510 520 530 540 550
VAQYEETLKQ QNGVRQKDQE EIRPIIPLVS CINGFVEPYR VEDFLSPATG
560 570 580 590 600
VKTFSLNSSR MATFPEVLII HLKKYTYNAD YTPKKLNVFM DVPDIIDIDS
610 620 630 640 650
LRGRGIKEGE VPLKEGTINT TTKVPEPSFN QEVLDTLLSM DFPLVRCKKA
660 670 680 690 700
LLATGGKDAE LAMNWIFEHT EDPDIDIEQT PVNNNNNNNN SSNSNDKLFV
710 720 730 740 750
FNSQDVDNII GMGFTDSQAK LALKNTKGNL ERAADWLFSH IDNLDELVAK
760 770 780 790 800
DNASTSSINP SLIPQSTTSL QPVSDGVGKY ELLGFISHLG NNVTCGHYVC
810 820 830
HIKKNNRWIK FNDRHVQLSE QPPKELGYIY FYKRQLN
Length:837
Mass (Da):94,966
Last modified:December 4, 2007 - v2
Checksum:i5E50A4E2CFE68BB4
GO

Sequence cautioni

The sequence EAL61603.2 differs from that shown. Reason: Erroneous gene model prediction.

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti180 – 1801T → N in AAC71068. (PubMed:9786928)Curated
Sequence conflicti683 – 6831N → I in AAC71068. (PubMed:9786928)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U48271 mRNA. Translation: AAC71068.1.
AAFI02000177 Genomic DNA. Translation: EAL61603.2. Sequence problems.
RefSeqiXP_635198.2. XM_630106.2.

Genome annotation databases

GeneIDi8628143.
KEGGiddi:DDB_G0291239.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U48271 mRNA. Translation: AAC71068.1 .
AAFI02000177 Genomic DNA. Translation: EAL61603.2 . Sequence problems.
RefSeqi XP_635198.2. XM_630106.2.

3D structure databases

ProteinModelPortali P54201.
SMRi P54201. Positions 167-276.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 44689.DDB_0231492.

Protein family/group databases

MEROPSi C19.104.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 8628143.
KEGGi ddi:DDB_G0291239.

Organism-specific databases

dictyBasei DDB_G0291239. ubpA.

Phylogenomic databases

eggNOGi COG5207.
InParanoidi P54201.
KOi K11836.
PhylomeDBi P54201.

Family and domain databases

Gene3Di 3.30.40.10. 1 hit.
InterProi IPR018200. Pept_C19ubi-hydrolase_C_CS.
IPR001394. Peptidase_C19_UCH.
IPR009060. UBA-like.
IPR015940. UBA/transl_elong_EF1B_N_euk.
IPR000449. UBA/Ts_N.
IPR016652. Ubiquitinyl_hydrolase.
IPR028889. UCH/PAN2.
IPR013083. Znf_RING/FYVE/PHD.
IPR001607. Znf_UBP.
[Graphical view ]
Pfami PF00627. UBA. 2 hits.
PF00443. UCH. 1 hit.
PF02148. zf-UBP. 1 hit.
[Graphical view ]
PIRSFi PIRSF016308. UBP. 1 hit.
SMARTi SM00165. UBA. 2 hits.
SM00290. ZnF_UBP. 1 hit.
[Graphical view ]
SUPFAMi SSF46934. SSF46934. 1 hit.
PROSITEi PS50030. UBA. 2 hits.
PS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
PS50271. ZF_UBP. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "A deubiquitinating enzyme that disassembles free polyubiquitin chains is required for development but not growth in Dictyostelium."
    Lindsey D.F., Amerik A., Deery W.J., Bishop J.D., Hochstrasser M., Gomer R.H.
    J. Biol. Chem. 273:29178-29187(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION.
  2. "The genome of the social amoeba Dictyostelium discoideum."
    Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
    , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
    Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AX4.

Entry informationi

Entry nameiUBPA_DICDI
AccessioniPrimary (citable) accession number: P54201
Secondary accession number(s): Q54EP2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: December 4, 2007
Last modified: October 29, 2014
This is version 96 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3