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P54201

- UBPA_DICDI

UniProt

P54201 - UBPA_DICDI

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Protein
Ubiquitin carboxyl-terminal hydrolase A
Gene
ubpA, usp5, DDB_G0291239
Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at transcript leveli

Functioni

Required for development but not growth.1 Publication

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei328 – 3281Nucleophile By similarity
Active sitei797 – 7971Proton acceptor By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri189 – 26375UBP-type
Add
BLAST

GO - Molecular functioni

  1. cysteine-type peptidase activity Source: UniProtKB-KW
  2. omega peptidase activity Source: InterPro
  3. ubiquitinyl hydrolase activity Source: InterPro
  4. zinc ion binding Source: InterPro

GO - Biological processi

  1. ubiquitin-dependent protein catabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Biological processi

Ubl conjugation pathway

Keywords - Ligandi

Metal-binding, Zinc

Protein family/group databases

MEROPSiC19.104.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin carboxyl-terminal hydrolase A (EC:3.4.19.12)
Alternative name(s):
Deubiquitinating enzyme A
Ubiquitin thioesterase A
Ubiquitin-specific-processing protease A
Gene namesi
Name:ubpA
Synonyms:usp5
ORF Names:DDB_G0291239
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
ProteomesiUP000002195: Chromosome 6, UP000002195: Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0291239. ubpA.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 837837Ubiquitin carboxyl-terminal hydrolase A
PRO_0000080686Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi44689.DDB_0231492.

Structurei

3D structure databases

ProteinModelPortaliP54201.
SMRiP54201. Positions 167-276.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini319 – 835517USP
Add
BLAST
Domaini628 – 66942UBA 1
Add
BLAST
Domaini700 – 74041UBA 2
Add
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi684 – 6907Poly-Asn

Sequence similaritiesi

Belongs to the peptidase C19 family.
Contains 2 UBA domains.
Contains 1 USP domain.

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiCOG5207.
KOiK11836.
PhylomeDBiP54201.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiIPR018200. Pept_C19ubi-hydrolase_C_CS.
IPR001394. Peptidase_C19_UCH.
IPR009060. UBA-like.
IPR015940. UBA/transl_elong_EF1B_N_euk.
IPR000449. UBA/Ts_N.
IPR016652. Ubiquitinyl_hydrolase.
IPR028889. UCH/PAN2.
IPR013083. Znf_RING/FYVE/PHD.
IPR001607. Znf_UBP.
[Graphical view]
PfamiPF00627. UBA. 2 hits.
PF00443. UCH. 1 hit.
PF02148. zf-UBP. 1 hit.
[Graphical view]
PIRSFiPIRSF016308. UBP. 1 hit.
SMARTiSM00165. UBA. 2 hits.
SM00290. ZnF_UBP. 1 hit.
[Graphical view]
SUPFAMiSSF46934. SSF46934. 1 hit.
PROSITEiPS50030. UBA. 2 hits.
PS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
PS50271. ZF_UBP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P54201-1 [UniParc]FASTAAdd to Basket

« Hide

MELFPELKNI KVPTEKSRLW KDECCYCFDT PENGEGLFID LIGLLAFSKK    50
YVQLNHQKTH HHLYLNFKKV AIVNEKVKSP TIENGGEEKP PKKLAIGVEG 100
GFNVEDEEIK YEEHYKLYIF PDDKFLELSD PIIPENVRVC CEKIKTLNSQ 150
SRKEEIVSWN AESVFPSAFA ESIIQLDNNT KKIDPKGPWR CDIEGCDKVE 200
NLWLNLTDGF IGCGRKYADG TGGNGHAQEH FNQTQYPISV KLGTITKDHA 250
DVYSYPEDDM VSDPLLFQHL THWGLNPNVM VKTEKSMAEL ELDQNLNFEF 300
GKIQEKGKLL ENVFGPGLTG IENLGNSCYM SSVIQMIFAI DSFQTRYLKD 350
REASFKDITQ DPTQSFEIQM SKLAHGLLSG DYSIPLSKPS KNANEESEAA 400
TQIGIAPKMF KSLIGASHAE FSTMKQQDAH EYLQYLLEYI ERAEHSRPSW 450
IQQANPTRLF QFHNEDRIEC GSSGQVKYTR RLENILSVPV NLDDATNKQE 500
VAQYEETLKQ QNGVRQKDQE EIRPIIPLVS CINGFVEPYR VEDFLSPATG 550
VKTFSLNSSR MATFPEVLII HLKKYTYNAD YTPKKLNVFM DVPDIIDIDS 600
LRGRGIKEGE VPLKEGTINT TTKVPEPSFN QEVLDTLLSM DFPLVRCKKA 650
LLATGGKDAE LAMNWIFEHT EDPDIDIEQT PVNNNNNNNN SSNSNDKLFV 700
FNSQDVDNII GMGFTDSQAK LALKNTKGNL ERAADWLFSH IDNLDELVAK 750
DNASTSSINP SLIPQSTTSL QPVSDGVGKY ELLGFISHLG NNVTCGHYVC 800
HIKKNNRWIK FNDRHVQLSE QPPKELGYIY FYKRQLN 837
Length:837
Mass (Da):94,966
Last modified:December 4, 2007 - v2
Checksum:i5E50A4E2CFE68BB4
GO

Sequence cautioni

The sequence EAL61603.2 differs from that shown. Reason: Erroneous gene model prediction.

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti180 – 1801T → N in AAC71068. 1 Publication
Sequence conflicti683 – 6831N → I in AAC71068. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U48271 mRNA. Translation: AAC71068.1.
AAFI02000177 Genomic DNA. Translation: EAL61603.2. Sequence problems.
RefSeqiXP_635198.2. XM_630106.2.

Genome annotation databases

GeneIDi8628143.
KEGGiddi:DDB_G0291239.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U48271 mRNA. Translation: AAC71068.1 .
AAFI02000177 Genomic DNA. Translation: EAL61603.2 . Sequence problems.
RefSeqi XP_635198.2. XM_630106.2.

3D structure databases

ProteinModelPortali P54201.
SMRi P54201. Positions 167-276.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 44689.DDB_0231492.

Protein family/group databases

MEROPSi C19.104.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 8628143.
KEGGi ddi:DDB_G0291239.

Organism-specific databases

dictyBasei DDB_G0291239. ubpA.

Phylogenomic databases

eggNOGi COG5207.
KOi K11836.
PhylomeDBi P54201.

Family and domain databases

Gene3Di 3.30.40.10. 1 hit.
InterProi IPR018200. Pept_C19ubi-hydrolase_C_CS.
IPR001394. Peptidase_C19_UCH.
IPR009060. UBA-like.
IPR015940. UBA/transl_elong_EF1B_N_euk.
IPR000449. UBA/Ts_N.
IPR016652. Ubiquitinyl_hydrolase.
IPR028889. UCH/PAN2.
IPR013083. Znf_RING/FYVE/PHD.
IPR001607. Znf_UBP.
[Graphical view ]
Pfami PF00627. UBA. 2 hits.
PF00443. UCH. 1 hit.
PF02148. zf-UBP. 1 hit.
[Graphical view ]
PIRSFi PIRSF016308. UBP. 1 hit.
SMARTi SM00165. UBA. 2 hits.
SM00290. ZnF_UBP. 1 hit.
[Graphical view ]
SUPFAMi SSF46934. SSF46934. 1 hit.
PROSITEi PS50030. UBA. 2 hits.
PS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
PS50271. ZF_UBP. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "A deubiquitinating enzyme that disassembles free polyubiquitin chains is required for development but not growth in Dictyostelium."
    Lindsey D.F., Amerik A., Deery W.J., Bishop J.D., Hochstrasser M., Gomer R.H.
    J. Biol. Chem. 273:29178-29187(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION.
  2. "The genome of the social amoeba Dictyostelium discoideum."
    Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
    , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
    Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AX4.

Entry informationi

Entry nameiUBPA_DICDI
AccessioniPrimary (citable) accession number: P54201
Secondary accession number(s): Q54EP2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: December 4, 2007
Last modified: September 3, 2014
This is version 94 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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