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P54201 (UBPA_DICDI) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 91. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ubiquitin carboxyl-terminal hydrolase A

EC=3.4.19.12
Alternative name(s):
Deubiquitinating enzyme A
Ubiquitin thioesterase A
Ubiquitin-specific-processing protease A
Gene names
Name:ubpA
Synonyms:usp5
ORF Names:DDB_G0291239
OrganismDictyostelium discoideum (Slime mold) [Reference proteome]
Taxonomic identifier44689 [NCBI]
Taxonomic lineageEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium

Protein attributes

Sequence length837 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Required for development but not growth. Ref.1

Catalytic activity

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sequence similarities

Belongs to the peptidase C19 family.

Contains 2 UBA domains.

Contains 1 UBP-type zinc finger.

Contains 1 USP domain.

Sequence caution

The sequence EAL61603.2 differs from that shown. Reason: Erroneous gene model prediction.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 837837Ubiquitin carboxyl-terminal hydrolase A
PRO_0000080686

Regions

Domain319 – 835517USP
Domain628 – 66942UBA 1
Domain700 – 74041UBA 2
Zinc finger189 – 26375UBP-type
Compositional bias684 – 6907Poly-Asn

Sites

Active site3281Nucleophile By similarity
Active site7971Proton acceptor By similarity

Experimental info

Sequence conflict1801T → N in AAC71068. Ref.1
Sequence conflict6831N → I in AAC71068. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P54201 [UniParc].

Last modified December 4, 2007. Version 2.
Checksum: 5E50A4E2CFE68BB4

FASTA83794,966
        10         20         30         40         50         60 
MELFPELKNI KVPTEKSRLW KDECCYCFDT PENGEGLFID LIGLLAFSKK YVQLNHQKTH 

        70         80         90        100        110        120 
HHLYLNFKKV AIVNEKVKSP TIENGGEEKP PKKLAIGVEG GFNVEDEEIK YEEHYKLYIF 

       130        140        150        160        170        180 
PDDKFLELSD PIIPENVRVC CEKIKTLNSQ SRKEEIVSWN AESVFPSAFA ESIIQLDNNT 

       190        200        210        220        230        240 
KKIDPKGPWR CDIEGCDKVE NLWLNLTDGF IGCGRKYADG TGGNGHAQEH FNQTQYPISV 

       250        260        270        280        290        300 
KLGTITKDHA DVYSYPEDDM VSDPLLFQHL THWGLNPNVM VKTEKSMAEL ELDQNLNFEF 

       310        320        330        340        350        360 
GKIQEKGKLL ENVFGPGLTG IENLGNSCYM SSVIQMIFAI DSFQTRYLKD REASFKDITQ 

       370        380        390        400        410        420 
DPTQSFEIQM SKLAHGLLSG DYSIPLSKPS KNANEESEAA TQIGIAPKMF KSLIGASHAE 

       430        440        450        460        470        480 
FSTMKQQDAH EYLQYLLEYI ERAEHSRPSW IQQANPTRLF QFHNEDRIEC GSSGQVKYTR 

       490        500        510        520        530        540 
RLENILSVPV NLDDATNKQE VAQYEETLKQ QNGVRQKDQE EIRPIIPLVS CINGFVEPYR 

       550        560        570        580        590        600 
VEDFLSPATG VKTFSLNSSR MATFPEVLII HLKKYTYNAD YTPKKLNVFM DVPDIIDIDS 

       610        620        630        640        650        660 
LRGRGIKEGE VPLKEGTINT TTKVPEPSFN QEVLDTLLSM DFPLVRCKKA LLATGGKDAE 

       670        680        690        700        710        720 
LAMNWIFEHT EDPDIDIEQT PVNNNNNNNN SSNSNDKLFV FNSQDVDNII GMGFTDSQAK 

       730        740        750        760        770        780 
LALKNTKGNL ERAADWLFSH IDNLDELVAK DNASTSSINP SLIPQSTTSL QPVSDGVGKY 

       790        800        810        820        830 
ELLGFISHLG NNVTCGHYVC HIKKNNRWIK FNDRHVQLSE QPPKELGYIY FYKRQLN 

« Hide

References

« Hide 'large scale' references
[1]"A deubiquitinating enzyme that disassembles free polyubiquitin chains is required for development but not growth in Dictyostelium."
Lindsey D.F., Amerik A., Deery W.J., Bishop J.D., Hochstrasser M., Gomer R.H.
J. Biol. Chem. 273:29178-29187(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION.
[2]"The genome of the social amoeba Dictyostelium discoideum."
Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. expand/collapse author list , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: AX4.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U48271 mRNA. Translation: AAC71068.1.
AAFI02000177 Genomic DNA. Translation: EAL61603.2. Sequence problems.

3D structure databases

ProteinModelPortalP54201.
SMRP54201. Positions 167-276.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING44689.DDB_0231492.

Protein family/group databases

MEROPSC19.104.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

KEGGddi:DDB_G0291239.

Organism-specific databases

dictyBaseDDB_G0291239. ubpA.

Phylogenomic databases

eggNOGCOG5207.
KOK11836.
PhylomeDBP54201.
ProtClustDBCLSZ2429562.

Family and domain databases

Gene3D3.30.40.10. 1 hit.
InterProIPR018200. Pept_C19ubi-hydrolase_C_CS.
IPR001394. Peptidase_C19_UCH.
IPR009060. UBA-like.
IPR015940. UBA/transl_elong_EF1B_N_euk.
IPR000449. UBA/Ts_N.
IPR016652. Ubiquitinyl_hydrolase.
IPR028889. UCH/PAN2.
IPR013083. Znf_RING/FYVE/PHD.
IPR001607. Znf_UBP.
[Graphical view]
PfamPF00627. UBA. 2 hits.
PF00443. UCH. 1 hit.
PF02148. zf-UBP. 1 hit.
[Graphical view]
PIRSFPIRSF016308. UBP. 1 hit.
SMARTSM00165. UBA. 2 hits.
SM00290. ZnF_UBP. 1 hit.
[Graphical view]
SUPFAMSSF46934. SSF46934. 1 hit.
PROSITEPS50030. UBA. 2 hits.
PS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
PS50271. ZF_UBP. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameUBPA_DICDI
AccessionPrimary (citable) accession number: P54201
Secondary accession number(s): Q54EP2
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: December 4, 2007
Last modified: April 16, 2014
This is version 91 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries

Dictyostelium discoideum

Dictyostelium discoideum: entries, gene names and cross-references to dictyBase