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Protein

Ubiquitin carboxyl-terminal hydrolase A

Gene

ubpA

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Required for development but not growth.1 Publication

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei328NucleophilePROSITE-ProRule annotation1
Active sitei797Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri189 – 263UBP-typePROSITE-ProRule annotationAdd BLAST75

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Biological processi

Ubl conjugation pathway

Keywords - Ligandi

Metal-binding, Zinc

Protein family/group databases

MEROPSiC19.104.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin carboxyl-terminal hydrolase A (EC:3.4.19.12)
Alternative name(s):
Deubiquitinating enzyme A
Ubiquitin thioesterase A
Ubiquitin-specific-processing protease A
Gene namesi
Name:ubpA
Synonyms:usp5
ORF Names:DDB_G0291239
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
Proteomesi
  • UP000002195 Componentsi: Chromosome 6, Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0291239. ubpA.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000806861 – 837Ubiquitin carboxyl-terminal hydrolase AAdd BLAST837

Proteomic databases

PaxDbiP54201.

Interactioni

Protein-protein interaction databases

STRINGi44689.DDB0231492.

Structurei

3D structure databases

ProteinModelPortaliP54201.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini319 – 835USPAdd BLAST517
Domaini628 – 669UBA 1PROSITE-ProRule annotationAdd BLAST42
Domaini700 – 740UBA 2PROSITE-ProRule annotationAdd BLAST41

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi684 – 690Poly-Asn7

Sequence similaritiesi

Belongs to the peptidase C19 family.Curated
Contains 2 UBA domains.PROSITE-ProRule annotation
Contains 1 UBP-type zinc finger.PROSITE-ProRule annotation
Contains 1 USP domain.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri189 – 263UBP-typePROSITE-ProRule annotationAdd BLAST75

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG0944. Eukaryota.
COG5207. LUCA.
InParanoidiP54201.
KOiK11836.
PhylomeDBiP54201.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiIPR001394. Peptidase_C19_UCH.
IPR015940. UBA.
IPR009060. UBA-like.
IPR016652. Ubiquitinyl_hydrolase.
IPR018200. USP_CS.
IPR028889. USP_dom.
IPR013083. Znf_RING/FYVE/PHD.
IPR001607. Znf_UBP.
[Graphical view]
PfamiPF00627. UBA. 2 hits.
PF00443. UCH. 1 hit.
PF02148. zf-UBP. 1 hit.
[Graphical view]
PIRSFiPIRSF016308. UBP. 1 hit.
SMARTiSM00165. UBA. 2 hits.
SM00290. ZnF_UBP. 1 hit.
[Graphical view]
SUPFAMiSSF46934. SSF46934. 1 hit.
PROSITEiPS50030. UBA. 2 hits.
PS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
PS50271. ZF_UBP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P54201-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MELFPELKNI KVPTEKSRLW KDECCYCFDT PENGEGLFID LIGLLAFSKK
60 70 80 90 100
YVQLNHQKTH HHLYLNFKKV AIVNEKVKSP TIENGGEEKP PKKLAIGVEG
110 120 130 140 150
GFNVEDEEIK YEEHYKLYIF PDDKFLELSD PIIPENVRVC CEKIKTLNSQ
160 170 180 190 200
SRKEEIVSWN AESVFPSAFA ESIIQLDNNT KKIDPKGPWR CDIEGCDKVE
210 220 230 240 250
NLWLNLTDGF IGCGRKYADG TGGNGHAQEH FNQTQYPISV KLGTITKDHA
260 270 280 290 300
DVYSYPEDDM VSDPLLFQHL THWGLNPNVM VKTEKSMAEL ELDQNLNFEF
310 320 330 340 350
GKIQEKGKLL ENVFGPGLTG IENLGNSCYM SSVIQMIFAI DSFQTRYLKD
360 370 380 390 400
REASFKDITQ DPTQSFEIQM SKLAHGLLSG DYSIPLSKPS KNANEESEAA
410 420 430 440 450
TQIGIAPKMF KSLIGASHAE FSTMKQQDAH EYLQYLLEYI ERAEHSRPSW
460 470 480 490 500
IQQANPTRLF QFHNEDRIEC GSSGQVKYTR RLENILSVPV NLDDATNKQE
510 520 530 540 550
VAQYEETLKQ QNGVRQKDQE EIRPIIPLVS CINGFVEPYR VEDFLSPATG
560 570 580 590 600
VKTFSLNSSR MATFPEVLII HLKKYTYNAD YTPKKLNVFM DVPDIIDIDS
610 620 630 640 650
LRGRGIKEGE VPLKEGTINT TTKVPEPSFN QEVLDTLLSM DFPLVRCKKA
660 670 680 690 700
LLATGGKDAE LAMNWIFEHT EDPDIDIEQT PVNNNNNNNN SSNSNDKLFV
710 720 730 740 750
FNSQDVDNII GMGFTDSQAK LALKNTKGNL ERAADWLFSH IDNLDELVAK
760 770 780 790 800
DNASTSSINP SLIPQSTTSL QPVSDGVGKY ELLGFISHLG NNVTCGHYVC
810 820 830
HIKKNNRWIK FNDRHVQLSE QPPKELGYIY FYKRQLN
Length:837
Mass (Da):94,966
Last modified:December 4, 2007 - v2
Checksum:i5E50A4E2CFE68BB4
GO

Sequence cautioni

The sequence EAL61603 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti180T → N in AAC71068 (PubMed:9786928).Curated1
Sequence conflicti683N → I in AAC71068 (PubMed:9786928).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U48271 mRNA. Translation: AAC71068.1.
AAFI02000177 Genomic DNA. Translation: EAL61603.2. Sequence problems.
RefSeqiXP_635198.2. XM_630106.2.

Genome annotation databases

EnsemblProtistsiEAL61603; EAL61603; DDB_G0291239.
GeneIDi8628143.
KEGGiddi:DDB_G0291239.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U48271 mRNA. Translation: AAC71068.1.
AAFI02000177 Genomic DNA. Translation: EAL61603.2. Sequence problems.
RefSeqiXP_635198.2. XM_630106.2.

3D structure databases

ProteinModelPortaliP54201.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi44689.DDB0231492.

Protein family/group databases

MEROPSiC19.104.

Proteomic databases

PaxDbiP54201.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblProtistsiEAL61603; EAL61603; DDB_G0291239.
GeneIDi8628143.
KEGGiddi:DDB_G0291239.

Organism-specific databases

dictyBaseiDDB_G0291239. ubpA.

Phylogenomic databases

eggNOGiKOG0944. Eukaryota.
COG5207. LUCA.
InParanoidiP54201.
KOiK11836.
PhylomeDBiP54201.

Miscellaneous databases

PROiP54201.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiIPR001394. Peptidase_C19_UCH.
IPR015940. UBA.
IPR009060. UBA-like.
IPR016652. Ubiquitinyl_hydrolase.
IPR018200. USP_CS.
IPR028889. USP_dom.
IPR013083. Znf_RING/FYVE/PHD.
IPR001607. Znf_UBP.
[Graphical view]
PfamiPF00627. UBA. 2 hits.
PF00443. UCH. 1 hit.
PF02148. zf-UBP. 1 hit.
[Graphical view]
PIRSFiPIRSF016308. UBP. 1 hit.
SMARTiSM00165. UBA. 2 hits.
SM00290. ZnF_UBP. 1 hit.
[Graphical view]
SUPFAMiSSF46934. SSF46934. 1 hit.
PROSITEiPS50030. UBA. 2 hits.
PS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
PS50271. ZF_UBP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiUBPA_DICDI
AccessioniPrimary (citable) accession number: P54201
Secondary accession number(s): Q54EP2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: December 4, 2007
Last modified: November 2, 2016
This is version 110 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.