Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Serine/threonine-protein kinase MPS1

Gene

MPS1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the regulation of the onset of mitosis. Involved in a pathway that coordinates cell proliferation and differentiation. Implicated in spindle pole body (SPD) duplication. Dual specificity kinase that can phosphorylate serine, threonine and tyrosine residues. Phosphorylates the SPC29 and SPC110 spindle pole body components.2 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei468ATPPROSITE-ProRule annotation1
Active sitei563Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi446 – 454ATPPROSITE-ProRule annotation9

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • protein serine/threonine/tyrosine kinase activity Source: SGD
  • protein serine/threonine kinase activity Source: SGD

GO - Biological processi

  • mitotic spindle assembly checkpoint Source: SGD
  • protein autophosphorylation Source: SGD
  • protein localization to kinetochore Source: SGD
  • protein phosphorylation Source: SGD
  • regulation of attachment of spindle microtubules to kinetochore Source: SGD
  • sister chromatid biorientation Source: SGD
  • spindle assembly Source: SGD
  • spindle pole body duplication Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-29454-MONOMER.
BRENDAi2.7.12.1. 984.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase MPS1 (EC:2.7.12.2)
Alternative name(s):
Monopolar spindle protein 1
Regulatory cell proliferation kinase 1
Gene namesi
Name:MPS1
Synonyms:RPK1
Ordered Locus Names:YDL028C
ORF Names:D2785
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDL028C.
SGDiS000002186. MPS1.

Subcellular locationi

GO - Cellular componenti

  • condensed nuclear chromosome kinetochore Source: SGD
  • spindle pole body Source: SGD
Complete GO annotation...

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi580D → A: Loss of activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000863911 – 764Serine/threonine-protein kinase MPS1Add BLAST764

Post-translational modificationi

Autophosphorylated.

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP54199.
PRIDEiP54199.

PTM databases

iPTMnetiP54199.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
BEM1P293663EBI-11224,EBI-3508
NDC80P404604EBI-11224,EBI-25247
SLA1P327902EBI-11224,EBI-17313

Protein-protein interaction databases

BioGridi32028. 160 interactors.
DIPiDIP-5897N.
IntActiP54199. 37 interactors.
MINTiMINT-597563.

Structurei

3D structure databases

ProteinModelPortaliP54199.
SMRiP54199.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini440 – 720Protein kinasePROSITE-ProRule annotationAdd BLAST281

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi273 – 282Poly-Ser10
Compositional biasi309 – 315Poly-Ser7

Sequence similaritiesi

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

GeneTreeiENSGT00790000123008.
HOGENOMiHOG000248654.
InParanoidiP54199.
KOiK08866.
OMAiCVKVVHD.
OrthoDBiEOG092C116S.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR016242. Mps1.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
PIRSFiPIRSF000611. Ser/Thr_PK_MPS1. 1 hit.
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P54199-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTNSFHDYV DLKSRTNTRQ FSDDEEFTTP PKLSNFGSAL LSHTEKTSAS
60 70 80 90 100
EILSSHNNDK IANRLEEMDR SSSRSHPPPS MGNLTSGHTS TSSHSTLFGR
110 120 130 140 150
YLRNNHQTSM TTMNTSDIEI NVGNSLDKSF ERIRNLRQNM KEDITAKYAE
160 170 180 190 200
RRSKRFLISN RTTKLGPAKR AMTLTNIFDE DVPNSPNQPI NARETVELPL
210 220 230 240 250
EDSHQTNFKE TKRNTDYDSI DFGDLNPIQY IKKHNLPTSD LPLISQIYFD
260 270 280 290 300
KQREENRQAA LRKHSSRELL YKSRSSSSSL SSNNLLANKD NSITSNNGSQ
310 320 330 340 350
PRRKVSTGSS SSKSSIEIRR ALKENIDTSN NSNFNSPIHK IYKGISRNKD
360 370 380 390 400
SDSEKREVLR NISINANHAD NLLQQENKRL KRSLDDAITN ENINSKNLEV
410 420 430 440 450
FYHRPAPKPP VTKKVEIVEP AKSASLSNNR NIITVNDSQY EKIELLGRGG
460 470 480 490 500
SSRVYKVKGS GNRVYALKRV SFDAFDDSSI DGFKGEIELL EKLKDQKRVI
510 520 530 540 550
QLLDYEMGDG LLYLIMECGD HDLSQILNQR SGMPLDFNFV RFYTKEMLLC
560 570 580 590 600
IKVVHDAGIV HSDLKPANFV LVKGILKIID FGIANAVPEH TVNIYRETQI
610 620 630 640 650
GTPNYMAPEA LVAMNYTQNS ENQHEGNKWK VGRPSDMWSC GCIIYQMIYG
660 670 680 690 700
KPPYGSFQGQ NRLLAIMNPD VKIPFPEHTS NNEKIPKSAI ELMKACLYRN
710 720 730 740 750
PDKRWTVDKV LSSTFLQPFM ISGSIMEDLI RNAVRYGSEK PHISQDDLND
760
VVDTVLRKFA DYKI
Length:764
Mass (Da):86,772
Last modified:July 27, 2011 - v2
Checksum:i5A404F4F83A9D548
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti146A → S in AAA88731 (PubMed:8028580).Curated1
Sequence conflicti211 – 213TKR → RRE in CAA96461 (PubMed:9046088).Curated3
Sequence conflicti211 – 213TKR → RRE in CAA98587 (PubMed:9169867).Curated3

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L08909 Genomic DNA. Translation: AAA88731.1.
Z71781 Genomic DNA. Translation: CAA96461.1.
Z74076 Genomic DNA. Translation: CAA98587.1.
BK006938 Genomic DNA. Translation: DAA11824.2.
PIRiS67561.
RefSeqiNP_010256.2. NM_001180087.2.

Genome annotation databases

EnsemblFungiiYDL028C; YDL028C; YDL028C.
GeneIDi851533.
KEGGisce:YDL028C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L08909 Genomic DNA. Translation: AAA88731.1.
Z71781 Genomic DNA. Translation: CAA96461.1.
Z74076 Genomic DNA. Translation: CAA98587.1.
BK006938 Genomic DNA. Translation: DAA11824.2.
PIRiS67561.
RefSeqiNP_010256.2. NM_001180087.2.

3D structure databases

ProteinModelPortaliP54199.
SMRiP54199.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi32028. 160 interactors.
DIPiDIP-5897N.
IntActiP54199. 37 interactors.
MINTiMINT-597563.

PTM databases

iPTMnetiP54199.

Proteomic databases

MaxQBiP54199.
PRIDEiP54199.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYDL028C; YDL028C; YDL028C.
GeneIDi851533.
KEGGisce:YDL028C.

Organism-specific databases

EuPathDBiFungiDB:YDL028C.
SGDiS000002186. MPS1.

Phylogenomic databases

GeneTreeiENSGT00790000123008.
HOGENOMiHOG000248654.
InParanoidiP54199.
KOiK08866.
OMAiCVKVVHD.
OrthoDBiEOG092C116S.

Enzyme and pathway databases

BioCyciYEAST:G3O-29454-MONOMER.
BRENDAi2.7.12.1. 984.

Miscellaneous databases

PROiP54199.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR016242. Mps1.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
PIRSFiPIRSF000611. Ser/Thr_PK_MPS1. 1 hit.
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMPS1_YEAST
AccessioniPrimary (citable) accession number: P54199
Secondary accession number(s): D6VRW4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: July 27, 2011
Last modified: November 2, 2016
This is version 149 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.