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P54199 (MPS1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 127. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Serine/threonine-protein kinase MPS1

EC=2.7.12.2
Alternative name(s):
Monopolar spindle protein 1
Regulatory cell proliferation kinase 1
Gene names
Name:MPS1
Synonyms:RPK1
Ordered Locus Names:YDL028C
ORF Names:D2785
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length764 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Involved in the regulation of the onset of mitosis. Involved in a pathway that coordinates cell proliferation and differentiation. Implicated in spindle pole body (SPD) duplication. Dual specificity kinase that can phosphorylate serine, threonine and tyrosine residues. Phosphorylates the SPC29 and SPC110 spindle pole body components. Ref.6 Ref.7

Catalytic activity

ATP + a protein = ADP + a phosphoprotein.

Post-translational modification

Autophosphorylated.

Sequence similarities

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.

Contains 1 protein kinase domain.

Binary interactions

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 764764Serine/threonine-protein kinase MPS1
PRO_0000086391

Regions

Domain440 – 720281Protein kinase
Nucleotide binding446 – 4549ATP By similarity
Compositional bias273 – 28210Poly-Ser
Compositional bias309 – 3157Poly-Ser

Sites

Active site5631Proton acceptor By similarity
Binding site4681ATP By similarity

Experimental info

Mutagenesis5801D → A: Loss of activity. Ref.5
Sequence conflict1461A → S in AAA88731. Ref.1
Sequence conflict211 – 2133TKR → RRE in CAA96461. Ref.2
Sequence conflict211 – 2133TKR → RRE in CAA98587. Ref.3

Sequences

Sequence LengthMass (Da)Tools
P54199 [UniParc].

Last modified July 27, 2011. Version 2.
Checksum: 5A404F4F83A9D548

FASTA76486,772
        10         20         30         40         50         60 
MSTNSFHDYV DLKSRTNTRQ FSDDEEFTTP PKLSNFGSAL LSHTEKTSAS EILSSHNNDK 

        70         80         90        100        110        120 
IANRLEEMDR SSSRSHPPPS MGNLTSGHTS TSSHSTLFGR YLRNNHQTSM TTMNTSDIEI 

       130        140        150        160        170        180 
NVGNSLDKSF ERIRNLRQNM KEDITAKYAE RRSKRFLISN RTTKLGPAKR AMTLTNIFDE 

       190        200        210        220        230        240 
DVPNSPNQPI NARETVELPL EDSHQTNFKE TKRNTDYDSI DFGDLNPIQY IKKHNLPTSD 

       250        260        270        280        290        300 
LPLISQIYFD KQREENRQAA LRKHSSRELL YKSRSSSSSL SSNNLLANKD NSITSNNGSQ 

       310        320        330        340        350        360 
PRRKVSTGSS SSKSSIEIRR ALKENIDTSN NSNFNSPIHK IYKGISRNKD SDSEKREVLR 

       370        380        390        400        410        420 
NISINANHAD NLLQQENKRL KRSLDDAITN ENINSKNLEV FYHRPAPKPP VTKKVEIVEP 

       430        440        450        460        470        480 
AKSASLSNNR NIITVNDSQY EKIELLGRGG SSRVYKVKGS GNRVYALKRV SFDAFDDSSI 

       490        500        510        520        530        540 
DGFKGEIELL EKLKDQKRVI QLLDYEMGDG LLYLIMECGD HDLSQILNQR SGMPLDFNFV 

       550        560        570        580        590        600 
RFYTKEMLLC IKVVHDAGIV HSDLKPANFV LVKGILKIID FGIANAVPEH TVNIYRETQI 

       610        620        630        640        650        660 
GTPNYMAPEA LVAMNYTQNS ENQHEGNKWK VGRPSDMWSC GCIIYQMIYG KPPYGSFQGQ 

       670        680        690        700        710        720 
NRLLAIMNPD VKIPFPEHTS NNEKIPKSAI ELMKACLYRN PDKRWTVDKV LSSTFLQPFM 

       730        740        750        760 
ISGSIMEDLI RNAVRYGSEK PHISQDDLND VVDTVLRKFA DYKI 

« Hide

References

« Hide 'large scale' references
[1]"RPK1, an essential yeast protein kinase involved in the regulation of the onset of mitosis, shows homology to mammalian dual-specificity kinases."
Poch O., Schwob E., de Fraipont F., Camasses A., Bordonne R., Martin R.P.
Mol. Gen. Genet. 243:641-653(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 28383 / FL100 / VTT C-80102.
[2]"The sequence of a 36.7 kb segment on the left arm of chromosome IV from Saccharomyces cerevisiae reveals 20 non-overlapping open reading frames (ORFs) including SIT4, FAD1, NAM1, RNA11, SIR2, NAT1, PRP9, ACT2 and MPS1 and 11 new ORFs."
Saren A.-M., Laamanen P., Lejarcegui J.B., Paulin L.
Yeast 13:65-71(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]"The nucleotide sequence of Saccharomyces cerevisiae chromosome IV."
Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T. expand/collapse author list , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P.
Nature 387:75-78(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION, SEQUENCE REVISION TO 211-213.
Strain: ATCC 204508 / S288c.
[5]"Yeast spindle pole body duplication gene MPS1 encodes an essential dual specificity protein kinase."
Lauze E., Stoelcker B., Luca F.C., Weiss E., Schutz A.R., Winey M.
EMBO J. 14:1655-1663(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: CHARACTERIZATION, MUTAGENESIS OF ASP-580.
[6]"Yeast Mps1p phosphorylates the spindle pole component Spc110p in the N-terminal domain."
Friedman D.B., Kern J.W., Huneycutt B.J., Vinh D.B., Crawford D.K., Steiner E., Scheiltz D., Yates J. III, Resing K.A., Ahn N.G., Winey M., Davis T.N.
J. Biol. Chem. 276:17958-17967(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[7]"Budding yeast centrosome duplication requires stabilization of Spc29 via Mps1-mediated phosphorylation."
Holinger E.P., Old W.M., Giddings T.H. Jr., Wong C., Yates J.R. III, Winey M.
J. Biol. Chem. 284:12949-12955(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L08909 Genomic DNA. Translation: AAA88731.1.
Z71781 Genomic DNA. Translation: CAA96461.1.
Z74076 Genomic DNA. Translation: CAA98587.1.
BK006938 Genomic DNA. Translation: DAA11824.2.
PIRS67561.
RefSeqNP_010256.2. NM_001180087.2.

3D structure databases

ProteinModelPortalP54199.
SMRP54199. Positions 405-734.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid32028. 159 interactions.
DIPDIP-5897N.
IntActP54199. 37 interactions.
MINTMINT-597563.
STRING4932.YDL028C.

Proteomic databases

MaxQBP54199.
PaxDbP54199.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYDL028C; YDL028C; YDL028C.
GeneID851533.
KEGGsce:YDL028C.

Organism-specific databases

CYGDYDL028c.
SGDS000002186. MPS1.

Phylogenomic databases

eggNOGCOG0515.
GeneTreeENSGT00620000087848.
HOGENOMHOG000248654.
KOK08866.
OMACVKVVHD.
OrthoDBEOG71GB3B.

Enzyme and pathway databases

BioCycYEAST:G3O-29454-MONOMER.
BRENDA2.7.12.1. 984.

Gene expression databases

GenevestigatorP54199.

Family and domain databases

InterProIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMSSF56112. SSF56112. 1 hit.
PROSITEPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio968929.
PROP54199.

Entry information

Entry nameMPS1_YEAST
AccessionPrimary (citable) accession number: P54199
Secondary accession number(s): D6VRW4
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: July 27, 2011
Last modified: June 11, 2014
This is version 127 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome IV

Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families