Reviewed, UniProtKB/Swiss-Prot P54197 (CHI2_COCP7)

Names and origin

Protein names

Recommended name:
    Endochitinase 2
    EC=3.2.1.14

Gene names

Name:	CTS2
ORF Names:	CPC735_058480

Organism

Coccidioides posadasii (strain C735) (Valley fever fungus)

Taxonomic identifier

222929 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Eurotiomycetes › Eurotiomycetidae › Onygenales › mitosporic Onygenales › Coccidioides

Protein attributes

Sequence length	855 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	May be associated with endosporulation.
Catalytic activity	Random hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins.
Subcellular location	Cell membrane; Lipid-anchor › GPI-anchor Potential.
Sequence similarities	Belongs to the glycosyl hydrolase 18 family. Chitinase class II subfamily.
Sequence caution	The sequence AAA92642.1 differs from that shown. Reason: Frameshift at position 847. The sequence EER24478.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Chitin degradation Polysaccharide degradation
Cellular component	Cell membrane Membrane
Domain	Signal
Ligand	Chitin-binding
Molecular function	Glycosidase Hydrolase
PTM	GPI-anchor Glycoprotein Lipoprotein
Gene Ontology (GO)
Biological process	chitin catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	anchored to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	cation binding Inferred from electronic annotation. Source: InterPro chitin binding Inferred from electronic annotation. Source: UniProtKB-KW chitinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 22	22	Potential
	Chain	23 – 826	804	Endochitinase 2		PRO_0000011928
	Propeptide	827 – 855	29	Removed in mature form Potential		PRO_0000252289
Regions
	Compositional bias	346 – 800	455	Ser/Thr-rich
Amino acid modifications
	Lipidation	826	1	GPI-anchor amidated glycine Potential
	Glycosylation	90	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	P54197-1 [UniParc]. Last modified October 3, 2006. Version 2. Checksum: A53628680FE0CA9D	FASTA	855	90,609
	10 20 30 40 50 60 MGPTNILAAF IAVSSLFIQS LALNPYAKSN LAVYWGQGAG QNRLSYFCEK TSFDIIVVGF 70 80 90 100 110 120 INVFPDQGPA GWPGSNFGNQ CADSYYYTKN GTKTKLLDGC YQIKEDLPKC KALGKTILLS 130 140 150 160 170 180 LGGGAVHDFY EVKSEESALN FADFLWGAFG PLTPDWTGPR PFGEASVDGF DFDIEKGSNF 190 200 210 220 230 240 GYSIMVRRLR ELFLQDPLNR YYISAAPQCI MPDKYLSHAI SNSAFDFIFI QFYNNPSCSA 250 260 270 280 290 300 KRWVTNPKSV TYTVDDWVKY IRKSGNPLAK LFIGLPASKS AAAKEDYLTP GEATKIVSTY 310 320 330 340 350 360 MAKYPSTFGG MMVWEATASE NNKLGGLPYA DIMKEVLLRC DPDPPTSTVT STTSASTSTQ 370 380 390 400 410 420 TSSQSTTMET KTLSASTTPS SPSTVSPSST MQTTSTGSTS IETVTTRSQE PPSTTISTRS 430 440 450 460 470 480 ASTEPVTTRS QEPPSTTIST RSASTETVTT RSQEPPSTTI STWSASTETS TSSQDSPSTT 490 500 510 520 530 540 ISTKSAPTGT VTTRSQDLPS TTISTRSPET ETETATTKSQ GSPSITLSTR SSSAETVSTR 550 560 570 580 590 600 SQHSSSTTIS TKSAPTETGT TSEHSTSMPV STRSASTETV ITRSQNSDSQ SMTVSTRSPS 610 620 630 640 650 660 TESITTRSQG SPSETFSTKS VPVDTISTEL PSQTPTTIIT GTPSDPVSAP TTTVPPNPTL 670 680 690 700 710 720 TLAPSSSTTE DRTTITTIIT TSYVTVCPTG FTTVTITYTT TYCPETASLT PTQPPIPGAP 730 740 750 760 770 780 APPPDGWTTI VTVCPQCAPT PTTVTLTVPT RSAFLPARTE TRPVVTVVPV PENPIKNVKP 790 800 810 820 830 840 SESGDFVTVT TAAPATVTKT LEYNNPVDSD VNVQPTGGSS PVEFEGSAMT VRSMDVVAKA 850 LITAGAAVLG LFLGL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Isolation and characterization of two chitinase-encoding genes (cts1, cts2) from the fungus Coccidioides immitis." Pishko E.J., Kirkland T.N., Cole G.T. Gene 167:173-177(1995) [PubMed: 8566773] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Comparative genomic analyses of the human fungal pathogens Coccidioides and their relatives." Sharpton T.J., Stajich J.E., Rounsley S.D., Gardner M.J., Wortman J.R., Jordar V.S., Maiti R., Kodira C.D., Neafsey D.E., Zeng Q., Hung C.-Y., McMahan C., Muszewska A., Grynberg M., Mandel M.A., Kellner E.M., Barker B.M., Galgiani J.N. , Orbach M.J., Kirkland T.N., Cole G.T., Henn M.R., Birren B.W., Taylor J.W. Genome Res. 19:1722-1731(2009) [PubMed: 19717792] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	L41662 Genomic DNA. Translation: AAA92642.1. Frameshift. ACFW01000049 Genomic DNA. Translation: EER24478.1. Sequence problems.
3D structure databases
HSSP	HSSP built from PDB template 2HVM based on UniProtKB P23472.
ModBase	Search...
Protein family/group databases
CAZy	GH18. Glycoside Hydrolase Family 18.
Enzyme and pathway databases
BRENDA	3.2.1.14. 281080.
Family and domain databases
InterPro	IPR001223. Glyco_hydro18cat. IPR001579. Glyco_hydro_18_chit_AS. IPR013781. Glyco_hydro_sg_catalytic. [Graphical view]
Gene3D	G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
Pfam	PF00704. Glyco_hydro_18. 1 hit. [Graphical view]
PROSITE	PS01095. CHITINASE_18. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CHI2_COCP7

Accession

Primary (citable) accession number: P54197
Secondary accession number(s): C5PIX5

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 3, 2006
Last modified:	November 3, 2009
This is version 62 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)