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Protein

Processive diacylglycerol beta-glucosyltransferase

Gene

ugtP

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Processive glucosyltransferase involved in the biosynthesis of both the bilayer- and non-bilayer-forming membrane glucolipids. Is able to successively transfer up to three glucosyl residues to diacylglycerol (DAG), thereby catalyzing the formation of beta-monoglucosyl-DAG (3-O-(beta-D-glucopyranosyl)-1,2-diacyl-sn-glycerol), beta-diglucosyl-DAG (3-O-(beta-D-glucopyranosyl-beta-(1->6)-D-glucopyranosyl)-1,2-diacyl-sn-glycerol) and beta-triglucosyl-DAG (3-O-(beta-D-glucopyranosyl-beta-(1->6)-D-glucopyranosyl-beta-(1->6)-D-glucopyranosyl)-1,2-diacyl-sn-glycerol). Beta-diglucosyl-DAG is the predominant glycolipid found in Bacillales and is also used as a membrane anchor for lipoteichoic acid (LTA). Also seems to be able to form beta-tetraglucosyl-DAG, although this glycolipid has not been found in B.subtilis membrane. UgtP can only use UDP-glucose as sugar donor.UniRule annotation1 Publication

Catalytic activityi

UDP-alpha-D-glucose + 1,2-diacyl-3-O-(beta-D-glucopyranosyl)-sn-glycerol = 1,2-diacyl-3-O-(beta-D-glucopyranosyl-(1->6)-O-beta-D-glucopyranosyl)-sn-glycerol + UDP.1 Publication
UDP-alpha-D-glucose + 1,2-diacyl-3-O-(beta-D-glucopyranosyl-(1->6)-O-beta-D-glucopyranosyl)-sn-glycerol = 1,2-diacyl-3-O-(beta-D-glucopyranosyl-(1->6)-beta-D-glucopyranosyl-(1->6)-O-beta-D-glucopyranosyl)-sn-glycerol + UDP.1 Publication
UDP-glucose + 1,2-diacyl-sn-glycerol = UDP + 1,2-diacyl-3-O-(beta-D-glucopyranosyl)-sn-glycerol.UniRule annotation1 Publication

Pathwayi: diglucosyl-diacylglycerol biosynthesis

This protein is involved in the pathway diglucosyl-diacylglycerol biosynthesis, which is part of Glycolipid metabolism.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway diglucosyl-diacylglycerol biosynthesis and in Glycolipid metabolism.

GO - Molecular functioni

  • 1,2-diacylglycerol 3-glucosyltransferase activity Source: UniProtKB

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Carbohydrate metabolism, Lipid biosynthesis, Lipid metabolism

Enzyme and pathway databases

BioCyciBSUB:BSU21920-MONOMER.
BRENDAi2.4.1.315. 658.
UniPathwayiUPA00894.

Protein family/group databases

CAZyiGT28. Glycosyltransferase Family 28.

Names & Taxonomyi

Protein namesi
Recommended name:
Processive diacylglycerol beta-glucosyltransferaseUniRule annotation (EC:2.4.1.315)
Alternative name(s):
Beta-diglucosyldiacylglycerol synthaseUniRule annotation
Short name:
Beta-DGSUniRule annotation
Short name:
DGlcDAG synthaseUniRule annotation
Short name:
Glc2-DAG synthaseUniRule annotation
Beta-gentiobiosyldiacylglycerol synthaseUniRule annotation
Beta-monoglucosyldiacylglycerol synthaseUniRule annotation
Short name:
Beta-MGSUniRule annotation
Short name:
MGlcDAG synthaseUniRule annotation
Beta-triglucosyldiacylglycerol synthaseUniRule annotation
Short name:
TGlcDAG synthaseUniRule annotation
Diglucosyl diacylglycerol synthase (1,6-linking)
Glucosyl-beta-1,6-glucosyldiacylglycerol synthaseUniRule annotation
UDP glucosyltransferaseUniRule annotation
UDP-glucose:1,2-diacylglycerol-3-beta-D-glucosyltransferaseUniRule annotation
Gene namesi
Name:ugtPUniRule annotation
Synonyms:ypfP
Ordered Locus Names:BSU21920
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

Subcellular locationi

  • Cell membrane UniRule annotation

  • Note: Localized in both septal membrane.1 Publication

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000803141 – 382Processive diacylglycerol beta-glucosyltransferaseAdd BLAST382

Proteomic databases

PaxDbiP54166.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
ftsZP178653EBI-1567571,EBI-1569853

Protein-protein interaction databases

IntActiP54166. 2 interactors.
STRINGi224308.Bsubs1_010100012066.

Structurei

3D structure databases

ProteinModelPortaliP54166.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyltransferase 28 family. UgtP subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0707. LUCA.
HOGENOMiHOG000221565.
InParanoidiP54166.
KOiK03429.
OMAiYKAFYYS.
PhylomeDBiP54166.

Family and domain databases

HAMAPiMF_01280. Diacylglyc_glucosyltr. 1 hit.
InterProiIPR009695. Diacylglyc_glucosyltr_N.
IPR001296. Glyco_trans_1.
IPR023589. Pro_diacylglycrl_glcsylTrfase.
[Graphical view]
PfamiPF00534. Glycos_transf_1. 1 hit.
PF06925. MGDG_synth. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P54166-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNTNKRVLIL TANYGNGHVQ VAKTLYEQCV RLGFQHVTVS NLYQESNPIV
60 70 80 90 100
SEVTQYLYLK SFSIGKQFYR LFYYGVDKIY NKRKFNIYFK MGNKRLGELV
110 120 130 140 150
DEHQPDIIIN TFPMIVVPEY RRRTGRVIPT FNVMTDFCLH KIWVHENVDK
160 170 180 190 200
YYVATDYVKE KLLEIGTHPS NVKITGIPIR PQFEESMPVG PIYKKYNLSP
210 220 230 240 250
NKKVLLIMAG AHGVLKNVKE LCENLVKDDQ VQVVVVCGKN TALKESLSAL
260 270 280 290 300
EAENGDKLKV LGYVERIDEL FRITDCMITK PGGITLTEAT AIGVPVILYK
310 320 330 340 350
PVPGQEKENA NFFEDRGAAI VVNRHEEILE SVTSLLADED TLHRMKKNIK
360 370 380
DLHLANSSEV ILEDILKESE MMTAKQKAKV LS
Length:382
Mass (Da):43,562
Last modified:October 1, 1996 - v1
Checksum:i8A8B98593DAC6041
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L77246 Genomic DNA. Translation: AAA96624.1.
AL009126 Genomic DNA. Translation: CAB14110.1.
PIRiC69935.
RefSeqiNP_390075.1. NC_000964.3.
WP_003246153.1. NZ_JNCM01000036.1.

Genome annotation databases

EnsemblBacteriaiCAB14110; CAB14110; BSU21920.
GeneIDi939081.
KEGGibsu:BSU21920.
PATRICi18976189. VBIBacSub10457_2285.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L77246 Genomic DNA. Translation: AAA96624.1.
AL009126 Genomic DNA. Translation: CAB14110.1.
PIRiC69935.
RefSeqiNP_390075.1. NC_000964.3.
WP_003246153.1. NZ_JNCM01000036.1.

3D structure databases

ProteinModelPortaliP54166.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP54166. 2 interactors.
STRINGi224308.Bsubs1_010100012066.

Protein family/group databases

CAZyiGT28. Glycosyltransferase Family 28.

Proteomic databases

PaxDbiP54166.

Protocols and materials databases

DNASUi939081.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAB14110; CAB14110; BSU21920.
GeneIDi939081.
KEGGibsu:BSU21920.
PATRICi18976189. VBIBacSub10457_2285.

Phylogenomic databases

eggNOGiCOG0707. LUCA.
HOGENOMiHOG000221565.
InParanoidiP54166.
KOiK03429.
OMAiYKAFYYS.
PhylomeDBiP54166.

Enzyme and pathway databases

UniPathwayiUPA00894.
BioCyciBSUB:BSU21920-MONOMER.
BRENDAi2.4.1.315. 658.

Family and domain databases

HAMAPiMF_01280. Diacylglyc_glucosyltr. 1 hit.
InterProiIPR009695. Diacylglyc_glucosyltr_N.
IPR001296. Glyco_trans_1.
IPR023589. Pro_diacylglycrl_glcsylTrfase.
[Graphical view]
PfamiPF00534. Glycos_transf_1. 1 hit.
PF06925. MGDG_synth. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiUGTP_BACSU
AccessioniPrimary (citable) accession number: P54166
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: September 7, 2016
This is version 108 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.