P54155 (MSRB_BACSU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Peptide methionine sulfoxide reductase MsrB
EC=1.8.4.12

Alternative name(s):
Peptide-methionine (R)-S-oxide reductase

Gene names

Name:	msrB
Synonyms:	yppQ
Ordered Locus Names:	BSU21680

Organism

Bacillus subtilis

Taxonomic identifier

1423 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	143 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	Peptide-L-methionine + thioredoxin disulfide + H2O = peptide-L-methionine (R)-S-oxide + thioredoxin. HAMAP MF_01400
Sequence similarities	Belongs to the MsrB Met sulfoxide reductase family.

Ontologies

Keywords
Molecular function	Oxidoreductase
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Molecular function	peptide-methionine (R)-S-oxide reductase activity Inferred from electronic annotation. Source: EC peptide-methionine-(S)-S-oxide reductase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 143

143

Peptide methionine sulfoxide reductase MsrB HAMAP MF_01400

PRO_0000140262

Sites

Active site

115

1

Nucleophile By similarity

Secondary structure

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143

Helix Strand Turn

Details...

Helix

5 – 9

5

Helix

14 – 22

9

Beta strand

38 – 46

9

Beta strand

50 – 53

4

Turn

54 – 56

3

Beta strand

62 – 64

3

Beta strand

67 – 70

4

Beta strand

74 – 78

5

Beta strand

89 – 94

6

Beta strand

101 – 105

5

Beta strand

107 – 110

4

Beta strand

112 – 115

4

Helix

118 – 120

3

Beta strand

121 – 125

5

Turn

126 – 128

3

Helix

130 – 132

3

Helix

137 – 140

4

Sequences

	Sequence		Length	Mass (Da)
	P54155 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: EC29C9B017C68EED	FASTA	143	16,602
	10 20 30 40 50 60 MAYNKEEKIK SLNRMQYEVT QNNGTEPPFQ NEYWDHKEEG LYVDIVSGKP LFTSKDKFDS 70 80 90 100 110 120 QCGWPSFTKP IEEEVEEKLD TSHGMIRTEV RSRTADSHLG HVFNDGPGPN GLRYCINSAA 130 140 LRFVPKHKLK EEGYESYLHL FNK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Organization of the Bacillus subtilis 168 chromosome between kdg and the attachment site of the SP beta prophage: use of long accurate PCR and yeast artificial chromosomes for sequencing." Capuano V., Galleron N., Pujic P., Sorokin A., Ehrlich S.D. Microbiology 142:3005-3015(1996) [PubMed: 8969496] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 168 / Marburg / ATCC 6051 / DSM 10 / JCM 1465 / NBRC 13719 / NCIMB 3610 / VKM B-501.
[2]	"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis." Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A. Nature 390:249-256(1997) [PubMed: 9384377] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 168.
[3]	"Solution NMR structure of methionine sulfoxide reductase B using minimal constraint strategy; Northeast structural genomics target SR10." Northeast structural genomics consortium (NESG) Submitted (OCT-2006) to the PDB data bank Cited for: STRUCTURE BY NMR.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

L77246 Genomic DNA. Translation: AAA96648.1.
AL009126 Genomic DNA. Translation: CAB14086.1.

PIR

F69940.

RefSeq

NP_390051.1. NC_000964.3.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2KZN	NMR	-	A	1-143	[»]
3E0O	X-ray	2.60	A/B/C/D/E/F	1-143	[»]

ProteinModelPortal

P54155.

SMR

P54155. Positions 1-141.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

EBBACT00000002834; EBBACP00000002834; EBBACG00000002829.

GeneID

939102.

GenomeReviews

Gene locus BSU21680 in contig AL009126_GR.

KEGG

bsu:BSU21680.

NMPDR

fig|224308.1.peg.2174.

PATRIC

18976141. VBIBacSub10457_2261.

Organism-specific databases

GenoList

BSU21680. [Micado]

Phylogenomic databases

GeneTree

EBGT00050000000580.

HOGENOM

HBG715255.

OMA

FTDGPVD.

PhylomeDB

P54155.

ProtClustDB

PRK00222.

Enzyme and pathway databases

BioCyc

BSUB:BSU21680-MONOMER.

Family and domain databases

HAMAP

MF_01400. MsrB.
[Tree]

InterPro

IPR002579. Methionine_sulphoxide_MsrB.
IPR011057. Mss4-like.
[Graphical view]

Gene3D

G3DSA:2.170.150.20. MsrB. 1 hit.

KO

K07305.

Pfam

PF01641. SelR. 1 hit.
[Graphical view]

SUPFAM

SSF51316. Mss4_like. 1 hit.

TIGRFAMs

TIGR00357. TIGR00357. 1 hit.

ProtoNet

Search...

Entry information

Entry name

MSRB_BACSU

Accession

Primary (citable) accession number: P54155

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	January 25, 2012
This is version 94 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program