Reviewed, UniProtKB/Swiss-Prot P54154 (MSRA_BACSU)

Names and origin

Protein names

Recommended name:
    Peptide methionine sulfoxide reductase msrA
      Short name=Protein-methionine-S-oxide reductase
    EC=1.8.4.11
Alternative name(s):
    Peptide-methionine (S)-S-oxide reductase
      Short name=Peptide Met(O) reductase

Gene names

Name:	msrA
Synonyms:	yppP
Ordered Locus Names:	BSU21690

Organism

Bacillus subtilis [Complete proteome] [HAMAP]

Taxonomic identifier

1423 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	177 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Has an important function as a repair enzyme for proteins that have been inactivated by oxidation. Catalyzes the reversible oxidation-reduction of methionine sulfoxide in proteins to methionine. May deal with oxidative damage to alpha/beta-type SASP in spores. HAMAP MF_01401
Catalytic activity	Peptide-L-methionine + thioredoxin disulfide + H2O = peptide-L-methionine (S)-S-oxide + thioredoxin. HAMAP MF_01401 L-methionine + thioredoxin disulfide + H2O = L-methionine (S)-S-oxide + thioredoxin. HAMAP MF_01401
Sequence similarities	Belongs to the msrA Met sulfoxide reductase family.

Ontologies

Keywords
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW protein modification process Inferred from electronic annotation. Source: HAMAP
Molecular function	peptide-methionine-(S)-S-oxide reductase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 177	177	Peptide methionine sulfoxide reductase msrA HAMAP MF_01401		PRO_0000138530
Sites
	Active site	14	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	P54154-1 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: DFEA7ADF34357D03	FASTA	177	20,182
	10 20 30 40 50 60 MSEKKEIATF AGGCFWCMVK PFDEQPGIEK VVSGYTGGHT ENPTYEEVCS ETTGHREAVQ 70 80 90 100 110 120 ITFHPDVFPY EKLLELFWQQ IDPTDAGGQF ADRGSSYRAA IFYHNDKQKE LAEASKQRLA 130 140 150 160 170 ESGIFKDPIV TDILKAEPFY EAEGYHQHFY KKNPAHYQRY RTGSGRAGFI SEHWGAK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Organization of the Bacillus subtilis 168 chromosome between kdg and the attachment site of the SP beta prophage: use of long accurate PCR and yeast artificial chromosomes for sequencing." Capuano V., Galleron N., Pujic P., Sorokin A., Ehrlich S.D. Microbiology 142:3005-3015(1996) [PubMed: 8969496] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 168 / Marburg / ATCC 6051 / DSM 10 / JCM 1465 / NCIB 3610.
[2]	"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis." Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A. Nature 390:249-256(1997) [PubMed: 9384377] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 168.
[3]	"In vitro and in vivo oxidation of methionine residues in small, acid-soluble spore proteins from Bacillus species." Hayes C.S., Illades-Aguiar B., Casillas-Martinez L., Setlow P. J. Bacteriol. 180:2694-2700(1998) [PubMed: 9573155] [Abstract] Cited for: CHARACTERIZATION.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L77246 Genomic DNA. Translation: AAA96647.1. AL009126 Genomic DNA. Translation: CAB14087.1.
PIR	E69940.
RefSeq	NP_390052.1.
3D structure databases
SMR	P54154. Positions 3-157.
ModBase	Search...
Genome annotation databases
GeneID	939099.
GenomeReviews	Gene locus BSU21690 in contig AL009126_GR.
KEGG	bsu:BSU21690.
NMPDR	fig|224308.1.peg.2175.
Organism-specific databases
SubtiList	BG11627. msrA. [Micado]
CMR	Search...
Phylogenomic databases
HOGENOM	HBG748152.
OMA	KTYELAT.
PhylomeDB	P54154.
Enzyme and pathway databases
BRENDA	1.8.4.11. 150.
Family and domain databases
HAMAP	MF_01401. MsrA. [Tree]
InterPro	IPR002569. Peptide_Met_Sox_Rdtase_MsrA. [Graphical view]
Gene3D	G3DSA:3.30.1060.10. MsrA. 1 hit.
Pfam	PF01625. PMSR. 1 hit. [Graphical view]
TIGRFAMs	TIGR00401. msrA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MSRA_BACSU

Accession

Primary (citable) accession number: P54154

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	February 9, 2010
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)