P54150 (MSRA4_ARATH) Reviewed, UniProtKB/Swiss-Prot
Last modified
January 25, 2012.
Version 84.
History...
Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: Peptide methionine sulfoxide reductase A4, chloroplastic Short name=AtMSRA4 EC=1.8.4.11 Alternative name(s): Peptide-methionine (S)-S-oxide reductase Short name=Peptide Met(O) reductase Protein-methionine-S-oxide reductase | ||||||||
| Gene names |
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| Organism | Arabidopsis thaliana (Mouse-ear cress) | ||||||||
| Taxonomic identifier | 3702 [NCBI] | ||||||||
| Taxonomic lineage | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis |
Protein attributes
| Sequence length | 258 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at protein level |
General annotation (Comments)
| Function | Catalyzes the reduction of methionine sulfoxide (MetSO) to methionine in proteins. Plays a protective role against oxidative stress by restoring activity to proteins that have been inactivated by methionine oxidation. Prevents the methionine sulfoxidation of the heat shock protein HSP21 and its subsequent inactivation. MSRA family specifically reduces the MetSO S-enantiomer. Ref.6 Ref.7 |
| Catalytic activity | Peptide-L-methionine + thioredoxin disulfide + H2O = peptide-L-methionine (S)-S-oxide + thioredoxin. L-methionine + thioredoxin disulfide + H2O = L-methionine (S)-S-oxide + thioredoxin. |
| Subcellular location | |
| Tissue specificity | Expressed in rosette and cauline leaves, and at lower levels in stems and flowers (at protein level). Ref.6 |
| Induction | By light in etiolated seedlings (at protein level). Ref.6 |
| Sequence similarities | Belongs to the MsrA Met sulfoxide reductase family. |
| Biophysicochemical properties | pH dependence: Optimum pH is 7.0. Ref.6 |
Ontologies
| Keywords | |
|---|---|
| Cellular component | Chloroplast Plastid |
| Domain | Transit peptide |
| Molecular function | Oxidoreductase |
| PTM | Phosphoprotein |
| Technical term | Complete proteome Reference proteome |
| Gene Ontology (GO) | |
| Biological process | cellular response to oxidative stress Inferred from expression pattern. Source: TAIR protein metabolic processInferred from electronic annotation. Source: InterPro response to light stimulusInferred from expression pattern Ref.6. Source: UniProtKB |
| Cellular component | chloroplast envelope Inferred from direct assay. Source: TAIR chloroplast stromaInferred from direct assay Ref.6. Source: UniProtKB |
| Molecular function | peptide-methionine-(S)-S-oxide reductase activity Inferred from direct assay Ref.6. Source: UniProtKB |
| Complete GO annotation... | |
Binary interactions
With | Entry | #Exp. | IntAct | Notes |
|---|---|---|---|---|
| TRX3 | Q42403 | 1 | EBI-449349,EBI-449157 |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Transit peptide | 1 – 68 | 68 | Chloroplast Potential | ||||||
| Chain | 69 – 258 | 190 | Peptide methionine sulfoxide reductase A4, chloroplastic | PRO_0000138632 | |||||
Amino acid modifications | |||||||||
| Modified residue | 130 | 1 | Phosphoserine Ref.9 | ||||||
Experimental info | |||||||||
| Sequence conflict | 131 | 1 | Y → S in CAA65991. Ref.1 | ||||||
| Sequence conflict | 195 | 1 | E → D in AAK83645. Ref.4 | ||||||
| Sequence conflict | 195 | 1 | E → D in AAN46787. Ref.4 | ||||||
| Sequence conflict | 212 | 1 | R → K in AAM65092. Ref.5 | ||||||
| Sequence conflict | 225 | 1 | R → K in AAK83645. Ref.4 | ||||||
| Sequence conflict | 225 | 1 | R → K in AAN46787. Ref.4 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | Piffanelli P., Batchedler C., Murphy D.J. Submitted (APR-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: cv. Columbia. |
| [2] | "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana." Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B. McCombie W.R.Nature 402:769-777(1999) [PubMed: 10617198] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia. |
| [3] | The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia. |
| [4] | "Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. Ecker J.R.Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia. |
| [5] | "Full-length cDNA from Arabidopsis thaliana." Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A. Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. |
| [6] | "Differential regulation of plastidial and cytosolic isoforms of peptide methionine sulfoxide reductase in Arabidopsis." Sadanandom A., Poghosyan Z., Fairbairn D.J., Murphy D.J. Plant Physiol. 123:255-264(2000) [PubMed: 10806242] [Abstract] Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, TISSUE SPECIFICITY, INDUCTION. Strain: cv. Columbia. |
| [7] | "A peptide methionine sulfoxide reductase highly expressed in photosynthetic tissue in Arabidopsis thaliana can protect the chaperone-like activity of a chloroplast-localized small heat shock protein." Gustavsson N., Kokke B.P., Haerndahl U., Silow M., Bechtold U., Poghosyan Z., Murphy D., Boelens W.C., Sundby C. Plant J. 29:545-553(2002) [PubMed: 11874568] [Abstract] Cited for: FUNCTION. |
| [8] | "Plant methionine sulfoxide reductase A and B multigenic families." Rouhier N., Vieira Dos Santos C., Tarrago L., Rey P. Photosyn. Res. 89:247-262(2006) [PubMed: 17031545] [Abstract] Cited for: GENE FAMILY, NOMENCLATURE. |
| [9] | "Large-scale Arabidopsis phosphoproteome profiling reveals novel chloroplast kinase substrates and phosphorylation networks." Reiland S., Messerli G., Baerenfaller K., Gerrits B., Endler A., Grossmann J., Gruissem W., Baginsky S. Plant Physiol. 150:889-903(2009) [PubMed: 19376835] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-130, MASS SPECTROMETRY. Strain: cv. Columbia. Tissue: Seedling. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | X97326 Genomic DNA. Translation: CAA65991.1. AL035523 Genomic DNA. Translation: CAB36755.1. AL161562 Genomic DNA. Translation: CAB79422.1. CP002687 Genomic DNA. Translation: AEE85015.1. AY049303 mRNA. Translation: AAK83645.1. BT001033 mRNA. Translation: AAN46787.1. AY087550 mRNA. Translation: AAM65092.1. |
| IPI | IPI00543565. |
| PIR | T05534. |
| RefSeq | NP_194243.1. NM_118645.3. |
| UniGene | At.24140. |
3D structure databases | |
| HSSP | HSSP built from PDB template 1FF3 based on UniProtKB P27110. |
| ProteinModelPortal | P54150. |
| SMR | P54150. Positions 78-258. |
| ModBase | Search... |
Protein-protein interaction databases | |
| IntAct | P54150. 1 interaction. |
| STRING | P54150. |
Proteomic databases | |
| PRIDE | P54150. |
Protocols and materials databases | |
| StructuralBiologyKnowledgebase | Search... |
Genome annotation databases | |
| EnsemblPlants | AT4G25130.1; AT4G25130.1; AT4G25130. |
| GeneID | 828616. |
| GenomeReviews | Gene locus AT4G25130 in contig CT486007_GR. |
| KEGG | ath:AT4G25130. |
| NMPDR | fig|3702.1.peg.20418. |
Organism-specific databases | |
| TAIR | At4g25130. |
Phylogenomic databases | |
| eggNOG | KOG1635. |
| GeneTree | EPGT00070000029005. |
| HOGENOM | HBG748152. |
| InParanoid | P54150. |
| OMA | QQKLLNR. |
| PhylomeDB | P54150. |
| ProtClustDB | CLSN2915968. |
Enzyme and pathway databases | |
| BRENDA | 1.8.4.11. 399. |
Gene expression databases | |
| ArrayExpress | P54150. |
| Genevestigator | P54150. |
| GermOnline | AT4G25130. Arabidopsis thaliana. |
Family and domain databases | |
| InterPro | IPR002569. Peptide_Met_Sox_Rdtase_MsrA. [Graphical view] |
| Gene3D | G3DSA:3.30.1060.10. MsrA. 1 hit. |
| KO | K07304. |
| Pfam | PF01625. PMSR. 1 hit. [Graphical view] |
| SUPFAM | SSF55068. MsrA. 1 hit. |
| TIGRFAMs | TIGR00401. MsrA. 1 hit. |
| ProtoNet | Search... |
Entry information
| Entry name | MSRA4_ARATH | ||||||||
| Accession | Primary (citable) accession number: P54150 Secondary accession number(s): Q8LAX4, Q94A72, Q9SW13 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Plant Protein Annotation Program | ||||||||
Relevant documents
| Arabidopsis thaliana Arabidopsis thaliana: entries and gene names |
| SIMILARITY comments Index of protein domains and families |

Clusters with