Reviewed, UniProtKB/Swiss-Prot P54149 (MSRA_BOVIN)

Names and origin

Protein names

Recommended name:
    Peptide methionine sulfoxide reductase
    EC=1.8.4.11
Alternative name(s):
    Protein-methionine-S-oxide reductase
    Peptide-methionine (S)-S-oxide reductase
      Short name=Peptide Met(O) reductase

Gene names

Name:

MSRA

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	233 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Has an important function as a repair enzyme for proteins that have been inactivated by oxidation. Catalyzes the reversible oxidation-reduction of methionine sulfoxide in proteins to methionine.
Catalytic activity	Peptide-L-methionine + thioredoxin disulfide + H(2)O = peptide-L-methionine (S)-S-oxide + thioredoxin. L-methionine + thioredoxin disulfide + H(2)O = L-methionine (S)-S-oxide + thioredoxin.
Sequence similarities	Belongs to the msrA Met sulfoxide reductase family.

Ontologies

Keywords
Molecular function	Oxidoreductase
Technical term	3D-structure
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW protein metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	peptide-methionine-(S)-S-oxide reductase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 233

233

Peptide methionine sulfoxide reductase

PRO_0000138625

Experimental info

Sequence conflict

4

1

A → V in AAC48539. Ref.1

Sequence conflict

125

1

H → R in AAX09061. Ref.2

Secondary structure

1

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233

Helix Strand Turn

Details...

Turn

31 – 33

3

Turn

49 – 51

3

Beta strand

54 – 58

5

Beta strand

64 – 72

9

Helix

73 – 81

9

Beta strand

86 – 100

15

Helix

103 – 107

5

Beta strand

114 – 121

8

Turn

123 – 125

3

Helix

128 – 137

10

Beta strand

143 – 147

5

Beta strand

150 – 152

3

Helix

153 – 155

3

Beta strand

157 – 159

3

Helix

164 – 183

20

Helix

204 – 206

3

Turn

207 – 211

5

Sequences

	Sequence		Length	Mass (Da)
	P54149-1 [UniParc]. Last modified May 30, 2006. Version 2. Checksum: DA09151E42FB6C08	FASTA	233	25,818
	10 20 30 40 50 60 MLSATRRALQ LFHSLFPIPR MGDSAAKIVS PQEALPGRKE PLVVAAKHHV NGNRTVEPFP 70 80 90 100 110 120 EGTQMAVFGM GCFWGAERKF WTLKGVYSTQ VGFAGGYTPN PTYKEVCSGK TGHAEVVRVV 130 140 150 160 170 180 FQPEHISFEE LLKVFWENHD PTQGMRQGND HGSQYRSAIY PTSAEHVGAA LKSKEDYQKV 190 200 210 220 230 LSEHGFGLIT TDIREGQTFY YAEDYHQQYL SKDPDGYCGL GGTGVSCPLG IKK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning the expression of a mammalian gene involved in the reduction of methionine sulfoxide residues in proteins." Moskovitz J., Weissbach H., Brot N. Proc. Natl. Acad. Sci. U.S.A. 93:2095-2099(1996) [PubMed: 8700890] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Adrenal medulla.
[2]	"Characterization of 954 bovine full-CDS cDNA sequences." Harhay G.P., Sonstegard T.S., Keele J.W., Heaton M.P., Clawson M.L., Snelling W.M., Wiedmann R.T., Van Tassell C.P., Smith T.P.L. BMC Genomics 6:166-166(2005) [PubMed: 16305752] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[3]	NIH - Mammalian Gene Collection (MGC) project Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Hereford. Tissue: Hypothalamus.
[4]	"Structure and mechanism of peptide methionine sulfoxide reductase, an 'anti-oxidation' enzyme." Lowther W.T., Brot N., Weissbach H., Matthews B.W. Biochemistry 39:13307-13312(2000) [PubMed: 11063566] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.6 ANGSTROMS).

Cross-references

Sequence databases

EMBLGenBankDDBJ

U37150 mRNA. Translation: AAC48539.1.
BT021044 mRNA. Translation: AAX09061.1.
BC102980 mRNA. Translation: AAI02981.1.

RefSeq

NP_776539.1.

UniGene

Bt.4655

3D structure databases

PDBMSD

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1FVA	X-ray	1.70	A/B	13-229	[»]
1FVG	X-ray	1.60	A	21-219	[»]

ModBase

Search...

Genome annotation databases

GeneID

281312.

KEGG

bta:281312.

Phylogenomic databases

HOVERGEN

P54149.

Family and domain databases

InterPro

IPR002569. MsrA.
[Graphical view]

Gene3D

G3DSA:3.30.1060.10. MsrA. 1 hit.

Pfam

PF01625. PMSR. 1 hit.
[Graphical view]

ProDom

PD003489. PMSR. 1 hit.
[Graphical view] [Entries sharing at least one domain]

TIGRFAMs

TIGR00401. msrA. 1 hit.

ProtoNet

Search...

Entry information

Entry name

MSRA_BOVIN

Accession

Primary (citable) accession number: P54149
Secondary accession number(s): Q3ZC16, Q5E976

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	May 30, 2006
Last modified:	November 25, 2008
This is version 58 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)