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Protein

D-inositol 3-phosphate glycosyltransferase

Gene

mshA

Organism
Mycobacterium leprae (strain TN)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the transfer of a N-acetyl-glucosamine moiety to 1D-myo-inositol 3-phosphate to produce 1D-myo-inositol 2-acetamido-2-deoxy-glucopyranoside 3-phosphate in the mycothiol biosynthesis pathway.By similarity

Catalytic activityi

UDP-N-acetyl-D-glucosamine + 1D-myo-inositol 3-phosphate = UDP + 1-O-(2-acetamido-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol 3-phosphate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei5 – 511D-inositol 3-phosphateBy similarity
Binding sitei19 – 191UDP-GlcNAc; via amide nitrogenBy similarity
Binding sitei74 – 7411D-inositol 3-phosphateBy similarity
Binding sitei107 – 10711D-inositol 3-phosphateBy similarity
Binding sitei131 – 13111D-inositol 3-phosphateBy similarity
Binding sitei151 – 15111D-inositol 3-phosphateBy similarity
Binding sitei225 – 2251UDP-GlcNAcBy similarity
Binding sitei230 – 2301UDP-GlcNAcBy similarity
Binding sitei283 – 2831UDP-GlcNAc; via amide nitrogen and carbonyl oxygenBy similarity
Metal bindingi292 – 2921Magnesium; via carbonyl oxygenBy similarity
Metal bindingi293 – 2931Magnesium; via carbonyl oxygenBy similarity
Metal bindingi295 – 2951Magnesium; via carbonyl oxygenBy similarity
Binding sitei305 – 3051UDP-GlcNAcBy similarity
Binding sitei313 – 3131UDP-GlcNAcBy similarity
Metal bindingi319 – 3191MagnesiumBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Ligandi

Magnesium, Metal-binding

Protein family/group databases

CAZyiGT4. Glycosyltransferase Family 4.

Names & Taxonomyi

Protein namesi
Recommended name:
D-inositol 3-phosphate glycosyltransferase (EC:2.4.1.250)
Alternative name(s):
N-acetylglucosamine-inositol-phosphate N-acetylglucosaminyltransferase
Short name:
GlcNAc-Ins-P N-acetylglucosaminyltransferase
Gene namesi
Name:mshA
Ordered Locus Names:ML2443
ORF Names:B2168_C2_201, u2168f
OrganismiMycobacterium leprae (strain TN)
Taxonomic identifieri272631 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacterium
Proteomesi
  • UP000000806 Componenti: Chromosome

Organism-specific databases

LepromaiML2443.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 428428D-inositol 3-phosphate glycosyltransferasePRO_0000080320Add
BLAST

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi272631.ML2443.

Structurei

3D structure databases

ProteinModelPortaliP54138.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni11 – 122UDP-GlcNAc bindingBy similarity
Regioni16 – 2161D-inositol 3-phosphate bindingBy similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4107R5M. Bacteria.
COG0438. LUCA.
HOGENOMiHOG000077288.
KOiK15521.
OMAiHTMAKVK.
OrthoDBiPOG091H0AO3.

Family and domain databases

HAMAPiMF_01695. MshA. 1 hit.
InterProiIPR001296. Glyco_trans_1.
IPR028098. Glyco_trans_4-like_N.
IPR017814. Mycothiol_biosynthesis_MshA.
[Graphical view]
PfamiPF13439. Glyco_transf_4. 1 hit.
PF00534. Glycos_transf_1. 1 hit.
[Graphical view]
TIGRFAMsiTIGR03449. mycothiol_MshA. 1 hit.

Sequencei

Sequence statusi: Complete.

P54138-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLAVHTSPLA QPGIGDAGGM NVYVLQSALH LARRGIEVEI FTRATASADP
60 70 80 90 100
PIVWVAPGVL VRNVVAGPFE GLDKYDLPTQ LCAFAAGVLR AEAAHEPGYY
110 120 130 140 150
DIVHSHYWLS GQVGWLARDR WAVPLVHTAH TLAAVKNAAL ADGDAAEPPL
160 170 180 190 200
RSVGEQQVVD EADRMIVNTD DEARQLISIH RADPAKIDVA HPGVDLDMFR
210 220 230 240 250
PGDRRAARAA LGLPLDGNVV AFVGRIQPLK APDIVLRAAA KLPQVRIVVA
260 270 280 290 300
GGPSGSGLAS PDGLVRLADE LGITARVTFL PPQSRTNLAT VFQAADLVAV
310 320 330 340 350
PSYSESFGLV AVEAQACGTP VVAAAVGGLP VAVRDGVTGT LVFGHNVGHW
360 370 380 390 400
ADAVDQLLRL SAGPQARAIS RAAVVHAAQF SWDNTTDALL ASYRRAIGDF
410 420
TATRQHRVRD LVATRKPRRW ISRRGMGA
Length:428
Mass (Da):45,292
Last modified:April 27, 2001 - v2
Checksum:iA14F9F0187E3587C
GO

Sequence cautioni

The sequence AAA17228 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00018 Genomic DNA. Translation: AAA17228.1. Different initiation.
AL583925 Genomic DNA. Translation: CAC31960.1.
PIRiH87214.
S72892.
RefSeqiNP_302584.1. NC_002677.1.
WP_010908903.1. NC_002677.1.

Genome annotation databases

EnsemblBacteriaiCAC31960; CAC31960; CAC31960.
GeneIDi908203.
KEGGimle:ML2443.
PATRICi18059551. VBIMycLep78757_4690.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00018 Genomic DNA. Translation: AAA17228.1. Different initiation.
AL583925 Genomic DNA. Translation: CAC31960.1.
PIRiH87214.
S72892.
RefSeqiNP_302584.1. NC_002677.1.
WP_010908903.1. NC_002677.1.

3D structure databases

ProteinModelPortaliP54138.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi272631.ML2443.

Protein family/group databases

CAZyiGT4. Glycosyltransferase Family 4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAC31960; CAC31960; CAC31960.
GeneIDi908203.
KEGGimle:ML2443.
PATRICi18059551. VBIMycLep78757_4690.

Organism-specific databases

LepromaiML2443.

Phylogenomic databases

eggNOGiENOG4107R5M. Bacteria.
COG0438. LUCA.
HOGENOMiHOG000077288.
KOiK15521.
OMAiHTMAKVK.
OrthoDBiPOG091H0AO3.

Family and domain databases

HAMAPiMF_01695. MshA. 1 hit.
InterProiIPR001296. Glyco_trans_1.
IPR028098. Glyco_trans_4-like_N.
IPR017814. Mycothiol_biosynthesis_MshA.
[Graphical view]
PfamiPF13439. Glyco_transf_4. 1 hit.
PF00534. Glycos_transf_1. 1 hit.
[Graphical view]
TIGRFAMsiTIGR03449. mycothiol_MshA. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiMSHA_MYCLE
AccessioniPrimary (citable) accession number: P54138
Secondary accession number(s): Q9CB50
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: April 27, 2001
Last modified: September 7, 2016
This is version 94 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.