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Protein

Neuronal acetylcholine receptor subunit alpha-7

Gene

CHRNA7

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. The channel is blocked by alpha-bungarotoxin.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ion channel, Ligand-gated ion channel, Receptor

Keywords - Biological processi

Ion transport, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Neuronal acetylcholine receptor subunit alpha-7
Gene namesi
Name:CHRNA7
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Unplaced

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini20 – 227208ExtracellularSequence analysisAdd
BLAST
Transmembranei228 – 25225HelicalSequence analysisAdd
BLAST
Transmembranei259 – 27719HelicalSequence analysisAdd
BLAST
Transmembranei293 – 31422HelicalSequence analysisAdd
BLAST
Topological domaini315 – 466152CytoplasmicSequence analysisAdd
BLAST
Transmembranei467 – 48721HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane, Postsynaptic cell membrane, Synapse

Pathology & Biotechi

Chemistry

ChEMBLiCHEMBL3350221.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1919By similarityAdd
BLAST
Chaini20 – 499480Neuronal acetylcholine receptor subunit alpha-7PRO_0000000365Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi43 – 431N-linked (GlcNAc...)Sequence analysis
Glycosylationi87 – 871N-linked (GlcNAc...)Sequence analysis
Glycosylationi130 – 1301N-linked (GlcNAc...)Sequence analysis
Disulfide bondi147 ↔ 161By similarity
Disulfide bondi209 ↔ 210Associated with receptor activationBy similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiP54131.
PRIDEiP54131.

Expressioni

Tissue specificityi

At least in chromaffin cells.

Interactioni

Subunit structurei

Homopentamer. Interacts with RIC3; which is required for proper folding and assembly (By similarity). Homooligomer of the short form gives rise to unfunctional channels, as does coexpression of both long and short forms of the receptor.By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000020942.

Chemistry

BindingDBiP54131.

Structurei

3D structure databases

ProteinModelPortaliP54131.
SMRiP54131. Positions 224-258.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3645. Eukaryota.
ENOG410XQGR. LUCA.
HOGENOMiHOG000006756.
HOVERGENiHBG003756.
InParanoidiP54131.
KOiK04809.

Family and domain databases

Gene3Di1.20.120.370. 2 hits.
2.70.170.10. 1 hit.
InterProiIPR027361. Acetylcholine_rcpt_TM.
IPR006202. Neur_chan_lig-bd.
IPR006201. Neur_channel.
IPR006029. Neurotrans-gated_channel_TM.
IPR018000. Neurotransmitter_ion_chnl_CS.
IPR002394. Nicotinic_acetylcholine_rcpt.
[Graphical view]
PANTHERiPTHR18945. PTHR18945. 3 hits.
PfamiPF02931. Neur_chan_LBD. 1 hit.
PF02932. Neur_chan_memb. 1 hit.
[Graphical view]
PRINTSiPR00254. NICOTINICR.
PR00252. NRIONCHANNEL.
SUPFAMiSSF63712. SSF63712. 1 hit.
SSF90112. SSF90112. 1 hit.
TIGRFAMsiTIGR00860. LIC. 1 hit.
PROSITEiPS00236. NEUROTR_ION_CHANNEL. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform Long (identifier: P54131-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MRGSLCLALA ASILHVSLQG EFQRKLYKDL VKNYNPLERP VANDSLPLTV
60 70 80 90 100
YFSLSLLQIM DVDEKNQVLT TNIWLQMTWT DHYLQWNASE YPGVKTVRFP
110 120 130 140 150
DGQIWKPDIL LYNSADERFD ATFHTNVLVN SSGHCQYLPP GIFKSSCYID
160 170 180 190 200
VRWFPFDVQQ CKLKFGSWSY GGWSLDLQMQ EADISGYIPN GEWDLVGVLG
210 220 230 240 250
KRSEKFYECC KEPYPDVTFT VSIRRRTLYY GLNLLIPCVL ISALALLVFL
260 270 280 290 300
LPADSGEKIS LGITVLLSLT VFMLLVAEIM PATSDSVPLI AQYFASTMII
310 320 330 340 350
VGLSVVVTVI VLQYHHHDPD GGKMPKWTRV VLLNWCAWFL RMKRPGEDKV
360 370 380 390 400
RPACQHNERR CSLASVEMSA VAGPPATNGN LLYIGFRGLD TMHCAPTPDS
410 420 430 440 450
GVVCGRVACS PTHDEHLLHA GQPSEGDPDL AKILEEVRYI AHRFRCQDES
460 470 480 490
EAVCSEWKFA ACVVDRLCLM AFSVFTILCT IGILMSAPNF VEAVSKDFA
Length:499
Mass (Da):56,003
Last modified:October 1, 1996 - v1
Checksum:iAEE5D0B3820D42D5
GO
Isoform Short (identifier: P54131-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     262-290: Missing.

Show »
Length:470
Mass (Da):52,976
Checksum:iD223E8558685C2CC
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei262 – 29029Missing in isoform Short. 1 PublicationVSP_000075Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X93604 mRNA. Translation: CAA63802.1.
RefSeqiNP_776940.1. NM_174515.2. [P54131-1]
UniGeneiBt.88.

Genome annotation databases

GeneIDi282178.
KEGGibta:282178.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X93604 mRNA. Translation: CAA63802.1.
RefSeqiNP_776940.1. NM_174515.2. [P54131-1]
UniGeneiBt.88.

3D structure databases

ProteinModelPortaliP54131.
SMRiP54131. Positions 224-258.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000020942.

Chemistry

BindingDBiP54131.
ChEMBLiCHEMBL3350221.

Proteomic databases

PaxDbiP54131.
PRIDEiP54131.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi282178.
KEGGibta:282178.

Organism-specific databases

CTDi1139.

Phylogenomic databases

eggNOGiKOG3645. Eukaryota.
ENOG410XQGR. LUCA.
HOGENOMiHOG000006756.
HOVERGENiHBG003756.
InParanoidiP54131.
KOiK04809.

Miscellaneous databases

NextBioi20806007.

Family and domain databases

Gene3Di1.20.120.370. 2 hits.
2.70.170.10. 1 hit.
InterProiIPR027361. Acetylcholine_rcpt_TM.
IPR006202. Neur_chan_lig-bd.
IPR006201. Neur_channel.
IPR006029. Neurotrans-gated_channel_TM.
IPR018000. Neurotransmitter_ion_chnl_CS.
IPR002394. Nicotinic_acetylcholine_rcpt.
[Graphical view]
PANTHERiPTHR18945. PTHR18945. 3 hits.
PfamiPF02931. Neur_chan_LBD. 1 hit.
PF02932. Neur_chan_memb. 1 hit.
[Graphical view]
PRINTSiPR00254. NICOTINICR.
PR00252. NRIONCHANNEL.
SUPFAMiSSF63712. SSF63712. 1 hit.
SSF90112. SSF90112. 1 hit.
TIGRFAMsiTIGR00860. LIC. 1 hit.
PROSITEiPS00236. NEUROTR_ION_CHANNEL. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Alpha-bungarotoxin-sensitive nicotinic receptors on bovine chromaffin cells: molecular cloning, functional expression and alternative splicing of the alpha 7 subunit."
    Garcia-Guzman M., Sala F., Sala S., Campos-Caro A., Stuehmer W., Gutierrez L., Criado M.
    Eur. J. Neurosci. 7:647-655(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS LONG AND SHORT).
    Tissue: Adrenal medulla.

Entry informationi

Entry nameiACHA7_BOVIN
AccessioniPrimary (citable) accession number: P54131
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: May 11, 2016
This is version 119 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.