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P54123 (RNJ_SYNY3) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 88. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ribonuclease J

Short name=RNase J
EC=3.1.-.-
Gene names
Name:rnj
Ordered Locus Names:slr0551
OrganismSynechocystis sp. (strain PCC 6803 / Kazusa) [Reference proteome] [HAMAP]
Taxonomic identifier1111708 [NCBI]
Taxonomic lineageBacteriaCyanobacteriaOscillatoriophycideaeChroococcalesSynechocystis

Protein attributes

Sequence length640 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

An RNase that has endonuclease and possibly 5'-3' exonuclease activity. Involved in maturation of rRNA and in some organisms also mRNA maturation and/or decay By similarity. HAMAP-Rule MF_01491

Cofactor

Binds up to 2 Zn2+ ions per subunit. It is not clear if Zn2+ or Mg2+ is physiologically important By similarity.

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_01491

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_01491.

Sequence similarities

Belongs to the metallo-beta-lactamase superfamily. RNA-metabolizing metallo-beta-lactamase-like family. Bacterial RNase J subfamily.

Ontologies

Keywords
   Biological processrRNA processing
   Cellular componentCytoplasm
   LigandMetal-binding
RNA-binding
Zinc
   Molecular functionEndonuclease
Exonuclease
Hydrolase
Nuclease
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionRNA binding

Inferred from electronic annotation. Source: UniProtKB-KW

endonuclease activity

Inferred from electronic annotation. Source: UniProtKB-KW

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 640640Ribonuclease J HAMAP-Rule MF_01491
PRO_0000215273

Regions

Region368 – 3725Substrate binding By similarity

Sites

Metal binding751Zinc 1; catalytic By similarity
Metal binding771Zinc 1; catalytic By similarity
Metal binding791Zinc 2; catalytic By similarity
Metal binding801Zinc 2; catalytic By similarity
Metal binding1451Zinc 1; catalytic By similarity
Metal binding1671Zinc 1; catalytic By similarity
Metal binding1671Zinc 2; catalytic By similarity
Metal binding3941Zinc 2; catalytic By similarity

Sequences

Sequence LengthMass (Da)Tools
P54123 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: F4D1CC23EA656E6D

FASTA64070,427
        10         20         30         40         50         60 
MAKNTQTQAL KILPLGGLHE IGKNTCVFEY DDEILLLDAG LAFPTDDMHG VNVVLPDMTY 

        70         80         90        100        110        120 
LRENREKIKG MVVTHGHEDH IGGIAYHLKQ FDIPIIYGPR LAMALLRDKL EEAGMLERTN 

       130        140        150        160        170        180 
LQTVSPREMV RLGKSFVVEF IRNTHSIADS YCLAIHTPLG VVMHSGDFKI DHTPIDGEFF 

       190        200        210        220        230        240 
DLQKVAEYGE KGVLCLLSDS TNAEVPGITP SEASVIPNLD RVFSQAEGRL MVTTFASSVH 

       250        260        270        280        290        300 
RVNIILSLAQ KHQRKVAVVG RSMLNVIAHA RKLGYIKCPD NLFVPLKAAR NLPDQQQLIL 

       310        320        330        340        350        360 
TTGSQGEPLA AMTRISNGEH PQIKIRQGDT VVFSANPIPG NTIAVVNTID RLMMQGANVI 

       370        380        390        400        410        420 
YGKHQGIHVS GHASQEEHKM LLALTRPKFF VPVHGEHRML VKHSQMAQAQ GIPSENIVIV 

       430        440        450        460        470        480 
NNGDVIELTG DRIRVAGQVP SGIELVDQAG IVHESTMAER QQMAEDGLVT VAAALSKTGT 

       490        500        510        520        530        540 
LLAYPEVHCR GVVMTIQPKL LEELIVRTIE NFLTERWSEF THGSNGSTEV SWNALQKELE 

       550        560        570        580        590        600 
SSLQRLIKRE LQSSPMVLLM LQTDTPIELD QVPQNVSTVS ATSATPAPRK KVVLTKTPEP 

       610        620        630        640 
KVKAKPEKKV VTTAEPSAQP VSTTKVYRRS RKRSTTSVSS 

« Hide

References

[1]"Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. I. Sequence features in the 1 Mb region from map positions 64% to 92% of the genome."
Kaneko T., Tanaka A., Sato S., Kotani H., Sazuka T., Miyajima N., Sugiura M., Tabata S.
DNA Res. 2:153-166(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 27184 / PCC 6803 / N-1.
[2]"Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions."
Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y., Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T., Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S. expand/collapse author list , Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
DNA Res. 3:109-136(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: PCC 6803 / Kazusa.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BA000022 Genomic DNA. Translation: BAA10871.1.
PIRS76024.
RefSeqNP_442800.1. NC_000911.1.
YP_005652861.1. NC_017277.1.
YP_007452676.1. NC_020286.1.

3D structure databases

ProteinModelPortalP54123.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP54123. 4 interactions.
STRING1148.slr0551.

Proteomic databases

PaxDbP54123.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAA10871; BAA10871; BAA10871.
GeneID952079.
KEGGsyn:slr0551.
syy:SYNGTS_2908.
syz:MYO_129360.
PATRIC23843368. VBISynSp132158_3220.

Phylogenomic databases

eggNOGCOG0595.
KOK12574.
OMAKDEKHLI.
OrthoDBEOG6P5ZDC.
PhylomeDBP54123.

Family and domain databases

Gene3D3.60.15.10. 1 hit.
HAMAPMF_01491. RNase_J_bact.
InterProIPR001279. Beta-lactamas-like.
IPR011108. RMMBL.
IPR004613. RNase_J.
IPR001587. RNase_J_CS.
[Graphical view]
PANTHERPTHR11203:SF22. PTHR11203:SF22. 1 hit.
PfamPF00753. Lactamase_B. 1 hit.
PF07521. RMMBL. 1 hit.
[Graphical view]
PIRSFPIRSF004803. RnjA. 1 hit.
SMARTSM00849. Lactamase_B. 1 hit.
[Graphical view]
SUPFAMSSF56281. SSF56281. 1 hit.
TIGRFAMsTIGR00649. MG423. 1 hit.
PROSITEPS01292. UPF0036. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameRNJ_SYNY3
AccessionPrimary (citable) accession number: P54123
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: July 9, 2014
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Synechocystis PCC 6803

Synechocystis (strain PCC 6803): entries and gene names

SIMILARITY comments

Index of protein domains and families