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P54115

- ALDH6_YEAST

UniProt

P54115 - ALDH6_YEAST

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Protein

Magnesium-activated aldehyde dehydrogenase, cytosolic

Gene

ALD6

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Cytosolic aldehyde dehydrogenase which utilizes NADP+ as the preferred coenzyme. Performs the conversion of acetaldehyde to acetate.1 Publication

Catalytic activityi

An aldehyde + NADP+ + H2O = a carboxylate + NADPH.1 Publication

Kineticsi

  1. KM=40 µM for NADP (with acetaldehyde as cosubstrate)1 Publication
  2. KM=99 µM for NADP (with propionaldehyde as cosubstrate)1 Publication
  3. KM=17.4 mM for NAD (with propionaldehyde as cosubstrate)1 Publication
  4. KM=24 µM for acetaldehyde (with NADP as cosubstrate)1 Publication
  5. KM=30 µM for propionaldehyde (with NADP as cosubstrate)1 Publication
  6. KM=0.7 mM for propionaldehyde (with NAD as cosubstrate)1 Publication

Vmax=24 µmol/min/mg enzyme with acetaldehyde and NADP as substrates1 Publication

Vmax=14 µmol/min/mg enzyme with propionaldehyde and NADP as substrates1 Publication

Vmax=8.3 µmol/min/mg enzyme with propionaldehyde and NAD as substrates1 Publication

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei173 – 1731Transition state stabilizerBy similarity
Active sitei272 – 2721Proton acceptorPROSITE-ProRule annotation
Active sitei306 – 3061NucleophilePROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi249 – 2546NADBy similarity

GO - Molecular functioni

  1. aldehyde dehydrogenase (NADP+) activity Source: UniProtKB-EC
  2. aldehyde dehydrogenase [NAD(P)+] activity Source: SGD

GO - Biological processi

  1. acetate biosynthetic process Source: SGD
  2. ethanol catabolic process Source: UniProtKB-UniPathway
  3. NADPH regeneration Source: SGD
  4. response to salt stress Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

Magnesium, NADP

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-13664.
YEAST:MONOMER-13664.
YEAST:YPL061W-MONOMER.
UniPathwayiUPA00780; UER00768.

Names & Taxonomyi

Protein namesi
Recommended name:
Magnesium-activated aldehyde dehydrogenase, cytosolic (EC:1.2.1.4)
Alternative name(s):
Mg(2+)-activated acetaldehyde dehydrogenase
Short name:
Mg(2+)-ACDH
Gene namesi
Name:ALD6
Synonyms:ALDH1
Ordered Locus Names:YPL061W
ORF Names:LPE9
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XVI

Organism-specific databases

SGDiS000005982. ALD6.

Subcellular locationi

GO - Cellular componenti

  1. cytosol Source: SGD
  2. mitochondrion Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 500499Magnesium-activated aldehyde dehydrogenase, cytosolicPRO_0000056441Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Cross-linki3 – 3Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication

Keywords - PTMi

Isopeptide bond, Ubl conjugation

Proteomic databases

MaxQBiP54115.
PaxDbiP54115.
PeptideAtlasiP54115.
PRIDEiP54115.

Expressioni

Gene expression databases

GenevestigatoriP54115.

Interactioni

Protein-protein interaction databases

BioGridi36117. 77 interactions.
DIPiDIP-8324N.
IntActiP54115. 25 interactions.
MINTiMINT-500664.
STRINGi4932.YPL061W.

Structurei

3D structure databases

ProteinModelPortaliP54115.
SMRiP54115. Positions 28-500.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the aldehyde dehydrogenase family.Curated

Phylogenomic databases

eggNOGiCOG1012.
GeneTreeiENSGT00760000118999.
HOGENOMiHOG000271505.
InParanoidiP54115.
KOiK00128.
OMAiAHHRDKV.
OrthoDBiEOG7S226Z.

Family and domain databases

Gene3Di3.40.309.10. 1 hit.
3.40.605.10. 1 hit.
InterProiIPR016161. Ald_DH/histidinol_DH.
IPR016163. Ald_DH_C.
IPR029510. Ald_DH_CS_GLU.
IPR016162. Ald_DH_N.
IPR015590. Aldehyde_DH_dom.
[Graphical view]
PfamiPF00171. Aldedh. 1 hit.
[Graphical view]
SUPFAMiSSF53720. SSF53720. 1 hit.
PROSITEiPS00687. ALDEHYDE_DEHYDR_GLU. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P54115-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTKLHFDTAE PVKITLPNGL TYEQPTGLFI NNKFMKAQDG KTYPVEDPST
60 70 80 90 100
ENTVCEVSSA TTEDVEYAIE CADRAFHDTE WATQDPRERG RLLSKLADEL
110 120 130 140 150
ESQIDLVSSI EALDNGKTLA LARGDVTIAI NCLRDAAAYA DKVNGRTINT
160 170 180 190 200
GDGYMNFTTL EPIGVCGQII PWNFPIMMLA WKIAPALAMG NVCILKPAAV
210 220 230 240 250
TPLNALYFAS LCKKVGIPAG VVNIVPGPGR TVGAALTNDP RIRKLAFTGS
260 270 280 290 300
TEVGKSVAVD SSESNLKKIT LELGGKSAHL VFDDANIKKT LPNLVNGIFK
310 320 330 340 350
NAGQICSSGS RIYVQEGIYD ELLAAFKAYL ETEIKVGNPF DKANFQGAIT
360 370 380 390 400
NRQQFDTIMN YIDIGKKEGA KILTGGEKVG DKGYFIRPTV FYDVNEDMRI
410 420 430 440 450
VKEEIFGPVV TVAKFKTLEE GVEMANSSEF GLGSGIETES LSTGLKVAKM
460 470 480 490 500
LKAGTVWINT YNDFDSRVPF GGVKQSGYGR EMGEEVYHAY TEVKAVRIKL
Length:500
Mass (Da):54,414
Last modified:January 23, 2007 - v4
Checksum:i542AA956EFA0E676
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti121 – 1211L → FK in AAB01219. (PubMed:9473035)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U56604 Genomic DNA. Translation: AAB01219.1.
U39205 Genomic DNA. Translation: AAB68304.1.
BK006949 Genomic DNA. Translation: DAA11369.1.
PIRiS60929.
RefSeqiNP_015264.1. NM_001183875.1.

Genome annotation databases

EnsemblFungiiYPL061W; YPL061W; YPL061W.
GeneIDi856044.
KEGGisce:YPL061W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U56604 Genomic DNA. Translation: AAB01219.1 .
U39205 Genomic DNA. Translation: AAB68304.1 .
BK006949 Genomic DNA. Translation: DAA11369.1 .
PIRi S60929.
RefSeqi NP_015264.1. NM_001183875.1.

3D structure databases

ProteinModelPortali P54115.
SMRi P54115. Positions 28-500.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 36117. 77 interactions.
DIPi DIP-8324N.
IntActi P54115. 25 interactions.
MINTi MINT-500664.
STRINGi 4932.YPL061W.

Proteomic databases

MaxQBi P54115.
PaxDbi P54115.
PeptideAtlasi P54115.
PRIDEi P54115.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YPL061W ; YPL061W ; YPL061W .
GeneIDi 856044.
KEGGi sce:YPL061W.

Organism-specific databases

SGDi S000005982. ALD6.

Phylogenomic databases

eggNOGi COG1012.
GeneTreei ENSGT00760000118999.
HOGENOMi HOG000271505.
InParanoidi P54115.
KOi K00128.
OMAi AHHRDKV.
OrthoDBi EOG7S226Z.

Enzyme and pathway databases

UniPathwayi UPA00780 ; UER00768 .
BioCyci MetaCyc:MONOMER-13664.
YEAST:MONOMER-13664.
YEAST:YPL061W-MONOMER.

Miscellaneous databases

NextBioi 980989.

Gene expression databases

Genevestigatori P54115.

Family and domain databases

Gene3Di 3.40.309.10. 1 hit.
3.40.605.10. 1 hit.
InterProi IPR016161. Ald_DH/histidinol_DH.
IPR016163. Ald_DH_C.
IPR029510. Ald_DH_CS_GLU.
IPR016162. Ald_DH_N.
IPR015590. Aldehyde_DH_dom.
[Graphical view ]
Pfami PF00171. Aldedh. 1 hit.
[Graphical view ]
SUPFAMi SSF53720. SSF53720. 1 hit.
PROSITEi PS00687. ALDEHYDE_DEHYDR_GLU. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning, characterization, and potential roles of cytosolic and mitochondrial aldehyde dehydrogenases in ethanol metabolism in Saccharomyces cerevisiae."
    Wang X., Mann C.J., Bai Y., Ni L., Weiner H.
    J. Bacteriol. 180:822-830(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES.
    Strain: DBY939.
  2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI."
    Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M.
    , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J.
    Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "Metabolic and regulatory changes associated with growth of Saccharomyces cerevisiae in 1.4 M NaCl. Evidence for osmotic induction of glycerol dissimilation via the dihydroxyacetone pathway."
    Norbeck J., Blomberg A.
    J. Biol. Chem. 272:5544-5554(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 76-79 AND 482-488.
    Strain: ATCC 44827 / SKQ2N.
  5. "The ALD6 gene of Saccharomyces cerevisiae encodes a cytosolic, Mg(2+)-activated acetaldehyde dehydrogenase."
    Meaden P.G., Dickinson F.M., Mifsud A., Tessier W., Westwater J., Bussey H., Midgley M.
    Yeast 13:1319-1327(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 2-16, CHARACTERIZATION.
  6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  7. Cited for: UBIQUITINATION [LARGE SCALE ANALYSIS] AT LYS-3.
    Strain: SUB592.
  8. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  9. "Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
    Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
    Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  10. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  11. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  12. "Sites of ubiquitin attachment in Saccharomyces cerevisiae."
    Starita L.M., Lo R.S., Eng J.K., von Haller P.D., Fields S.
    Proteomics 12:236-240(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiALDH6_YEAST
AccessioniPrimary (citable) accession number: P54115
Secondary accession number(s): D6W3V3, Q02782
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: January 23, 2007
Last modified: October 29, 2014
This is version 137 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 135000 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XVI
    Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

External Data

Dasty 3