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P54113 (PUR91_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 125. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Bifunctional purine biosynthesis protein ADE16

Including the following 2 domains:

  1. Phosphoribosylaminoimidazolecarboxamide formyltransferase
    EC=2.1.2.3
    Alternative name(s):
    5-aminoimidazole-4-carboxamide ribonucleotide formyltransferase
    AICAR transformylase
  2. IMP cyclohydrolase
    EC=3.5.4.10
    Alternative name(s):
    ATIC
    IMP synthase
    Inosinicase
Gene names
Name:ADE16
Ordered Locus Names:YLR028C
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length591 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

10-formyltetrahydrofolate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide = tetrahydrofolate + 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide.

IMP + H2O = 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide.

Pathway

Purine metabolism; IMP biosynthesis via de novo pathway; 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide (10-formyl THF route): step 1/1.

Purine metabolism; IMP biosynthesis via de novo pathway; IMP from 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide: step 1/1.

Subunit structure

Homodimer (Possible).

Domain

The IMP cyclohydrolase activity resides in the N-terminal region By similarity.

Miscellaneous

Present with 7700 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the PurH family.

Sequence caution

The sequence CAA97551.2 differs from that shown. Reason: Erroneous translation. Wrong choice of frame.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

ADE17P380093EBI-14213,EBI-14223

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 591591Bifunctional purine biosynthesis protein ADE16
PRO_0000192159

Sequences

Sequence LengthMass (Da)Tools
P54113 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: DEC06684BFED7CA7

FASTA59165,282
        10         20         30         40         50         60 
MGKYTKTAIL SVYDKTGLLD LAKGLVENNV RILASGGTAN MVREAGFPVD DVSSITHAPE 

        70         80         90        100        110        120 
MLGGRVKTLH PAVHAGILAR NLEGDEKDLK EQHIDKVDFV VCNLYPFKET VAKIGVTVQE 

       130        140        150        160        170        180 
AVEEIDIGGV TLLRAAAKNH SRVTILSDPN DYSIFLQDLS KDGEISQDLR NRFALKAFEH 

       190        200        210        220        230        240 
TADYDAAISD FFRKQYSEGK AQLPLRYGCN PHQRPAQAYI TQQEELPFKV LCGTPGYINL 

       250        260        270        280        290        300 
LDALNSWPLV KELSASLNLP AAASFKHVSP AGAAVGLPLS DVERQVYFVN DMEDLSPLAC 

       310        320        330        340        350        360 
AYARARGADR MSSFGDFIAL SNIVDVATAK IISKEVSDGV IAPGYEPEAL NILSKKKNGK 

       370        380        390        400        410        420 
YCILQIDPNY VPGQMESREV FGVTLQQKRN DAIINQSTFK EIVSKNKALT EQAVIDLTVA 

       430        440        450        460        470        480 
TLVLKYTQSN SVCYAKNGMV VGLGAGQQSR IHCTRLAGDK TDNWWLRQHP KVLNMKWAKG 

       490        500        510        520        530        540 
IKRADKSNAI DLFVTGQRIE GPEKVDYESK FEEVPEPFTK EERLEWLSKL NNVSLSSDAF 

       550        560        570        580        590 
FPFPDNVYRA VQSGVKFITA PSGSVMDKVV FQAADSFDIV YVENPIRLFH H 

« Hide

References

« Hide 'large scale' references
[1]Tibbetts A.S., Appling D.R.
Submitted (JUN-1996) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XII."
Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H. expand/collapse author list , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D.
Nature 387:87-90(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U62402 Genomic DNA. Translation: AAB57774.1.
Z73199 Genomic DNA. Translation: CAA97551.2. Sequence problems.
Z73200 Genomic DNA. Translation: CAA97552.1.
BK006945 Genomic DNA. Translation: DAA09346.1.
PIRS77707.
RefSeqNP_013128.1. NM_001181915.1.

3D structure databases

ProteinModelPortalP54113.
SMRP54113. Positions 6-591.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid31302. 33 interactions.
DIPDIP-4657N.
IntActP54113. 16 interactions.
MINTMINT-477775.
STRING4932.YLR028C.

Proteomic databases

MaxQBP54113.
PaxDbP54113.
PeptideAtlasP54113.
PRIDEP54113.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYLR028C; YLR028C; YLR028C.
GeneID850715.
KEGGsce:YLR028C.

Organism-specific databases

CYGDYLR028c.
SGDS000004018. ADE16.

Phylogenomic databases

eggNOGCOG0138.
GeneTreeENSGT00390000004553.
HOGENOMHOG000230372.
KOK00602.
OMAVMSERIP.
OrthoDBEOG7HXD0J.

Enzyme and pathway databases

BioCycMetaCyc:YLR028C-MONOMER.
YEAST:YLR028C-MONOMER.
UniPathwayUPA00074; UER00133.
UPA00074; UER00135.

Gene expression databases

GenevestigatorP54113.

Family and domain databases

Gene3D1.10.287.440. 1 hit.
3.40.140.20. 3 hits.
3.40.50.1380. 1 hit.
InterProIPR024051. AICAR_Tfase_dom.
IPR024050. AICAR_Tfase_insert_dom.
IPR002695. AICARFT_IMPCHas.
IPR016193. Cytidine_deaminase-like.
IPR011607. MGS-like_dom.
[Graphical view]
PANTHERPTHR11692. PTHR11692. 1 hit.
PfamPF01808. AICARFT_IMPCHas. 1 hit.
PF02142. MGS. 1 hit.
[Graphical view]
PIRSFPIRSF000414. AICARFT_IMPCHas. 1 hit.
SMARTSM00798. AICARFT_IMPCHas. 1 hit.
SM00851. MGS. 1 hit.
[Graphical view]
SUPFAMSSF52335. SSF52335. 1 hit.
SSF53927. SSF53927. 1 hit.
TIGRFAMsTIGR00355. purH. 1 hit.
ProtoNetSearch...

Other

NextBio966781.

Entry information

Entry namePUR91_YEAST
AccessionPrimary (citable) accession number: P54113
Secondary accession number(s): D6VY30, E9PAG6
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: June 11, 2014
This is version 125 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XII

Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways