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P54113

- PUR91_YEAST

UniProt

P54113 - PUR91_YEAST

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Protein

Bifunctional purine biosynthesis protein ADE16

Gene

ADE16

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Catalytic activityi

10-formyltetrahydrofolate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide = tetrahydrofolate + 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide.
IMP + H2O = 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide.

Pathwayi

GO - Molecular functioni

  1. IMP cyclohydrolase activity Source: SGD
  2. phosphoribosylaminoimidazolecarboxamide formyltransferase activity Source: SGD

GO - Biological processi

  1. 'de novo' IMP biosynthetic process Source: SGD
  2. aerobic respiration Source: SGD
  3. ascospore formation Source: SGD
  4. purine nucleotide biosynthetic process Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Transferase

Keywords - Biological processi

Purine biosynthesis

Enzyme and pathway databases

BioCyciMetaCyc:YLR028C-MONOMER.
YEAST:YLR028C-MONOMER.
UniPathwayiUPA00074; UER00133.
UPA00074; UER00135.

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional purine biosynthesis protein ADE16
Including the following 2 domains:
Phosphoribosylaminoimidazolecarboxamide formyltransferase (EC:2.1.2.3)
Alternative name(s):
5-aminoimidazole-4-carboxamide ribonucleotide formyltransferase
AICAR transformylase
IMP cyclohydrolase (EC:3.5.4.10)
Alternative name(s):
ATIC
IMP synthase
Inosinicase
Gene namesi
Name:ADE16
Ordered Locus Names:YLR028C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XII

Organism-specific databases

CYGDiYLR028c.
SGDiS000004018. ADE16.

Subcellular locationi

GO - Cellular componenti

  1. cytosol Source: SGD
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 591591Bifunctional purine biosynthesis protein ADE16PRO_0000192159Add
BLAST

Proteomic databases

MaxQBiP54113.
PaxDbiP54113.
PeptideAtlasiP54113.
PRIDEiP54113.

Expressioni

Gene expression databases

GenevestigatoriP54113.

Interactioni

Subunit structurei

Homodimer (Possible).

Binary interactionsi

WithEntry#Exp.IntActNotes
ADE17P380093EBI-14213,EBI-14223

Protein-protein interaction databases

BioGridi31302. 35 interactions.
DIPiDIP-4657N.
IntActiP54113. 16 interactions.
MINTiMINT-477775.
STRINGi4932.YLR028C.

Structurei

3D structure databases

ProteinModelPortaliP54113.
SMRiP54113. Positions 6-591.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domaini

The IMP cyclohydrolase activity resides in the N-terminal region.By similarity

Sequence similaritiesi

Belongs to the PurH family.Curated

Phylogenomic databases

eggNOGiCOG0138.
GeneTreeiENSGT00760000120248.
HOGENOMiHOG000230372.
InParanoidiP54113.
KOiK00602.
OMAiVMSERIP.
OrthoDBiEOG7HXD0J.

Family and domain databases

Gene3Di1.10.287.440. 1 hit.
3.40.140.20. 3 hits.
3.40.50.1380. 1 hit.
HAMAPiMF_00139. PurH.
InterProiIPR024051. AICAR_Tfase_dom.
IPR024050. AICAR_Tfase_insert_dom.
IPR002695. AICARFT_IMPCHas.
IPR016193. Cytidine_deaminase-like.
IPR011607. MGS-like_dom.
[Graphical view]
PANTHERiPTHR11692. PTHR11692. 1 hit.
PfamiPF01808. AICARFT_IMPCHas. 1 hit.
PF02142. MGS. 1 hit.
[Graphical view]
PIRSFiPIRSF000414. AICARFT_IMPCHas. 1 hit.
SMARTiSM00798. AICARFT_IMPCHas. 1 hit.
SM00851. MGS. 1 hit.
[Graphical view]
SUPFAMiSSF52335. SSF52335. 1 hit.
SSF53927. SSF53927. 1 hit.
TIGRFAMsiTIGR00355. purH. 1 hit.

Sequencei

Sequence statusi: Complete.

P54113 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MGKYTKTAIL SVYDKTGLLD LAKGLVENNV RILASGGTAN MVREAGFPVD
60 70 80 90 100
DVSSITHAPE MLGGRVKTLH PAVHAGILAR NLEGDEKDLK EQHIDKVDFV
110 120 130 140 150
VCNLYPFKET VAKIGVTVQE AVEEIDIGGV TLLRAAAKNH SRVTILSDPN
160 170 180 190 200
DYSIFLQDLS KDGEISQDLR NRFALKAFEH TADYDAAISD FFRKQYSEGK
210 220 230 240 250
AQLPLRYGCN PHQRPAQAYI TQQEELPFKV LCGTPGYINL LDALNSWPLV
260 270 280 290 300
KELSASLNLP AAASFKHVSP AGAAVGLPLS DVERQVYFVN DMEDLSPLAC
310 320 330 340 350
AYARARGADR MSSFGDFIAL SNIVDVATAK IISKEVSDGV IAPGYEPEAL
360 370 380 390 400
NILSKKKNGK YCILQIDPNY VPGQMESREV FGVTLQQKRN DAIINQSTFK
410 420 430 440 450
EIVSKNKALT EQAVIDLTVA TLVLKYTQSN SVCYAKNGMV VGLGAGQQSR
460 470 480 490 500
IHCTRLAGDK TDNWWLRQHP KVLNMKWAKG IKRADKSNAI DLFVTGQRIE
510 520 530 540 550
GPEKVDYESK FEEVPEPFTK EERLEWLSKL NNVSLSSDAF FPFPDNVYRA
560 570 580 590
VQSGVKFITA PSGSVMDKVV FQAADSFDIV YVENPIRLFH H
Length:591
Mass (Da):65,282
Last modified:October 1, 1996 - v1
Checksum:iDEC06684BFED7CA7
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U62402 Genomic DNA. Translation: AAB57774.1.
Z73199 Genomic DNA. Translation: CAA97551.2. Sequence problems.
Z73200 Genomic DNA. Translation: CAA97552.1.
BK006945 Genomic DNA. Translation: DAA09346.1.
PIRiS77707.
RefSeqiNP_013128.1. NM_001181915.1.

Genome annotation databases

EnsemblFungiiYLR028C; YLR028C; YLR028C.
GeneIDi850715.
KEGGisce:YLR028C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U62402 Genomic DNA. Translation: AAB57774.1 .
Z73199 Genomic DNA. Translation: CAA97551.2 . Sequence problems.
Z73200 Genomic DNA. Translation: CAA97552.1 .
BK006945 Genomic DNA. Translation: DAA09346.1 .
PIRi S77707.
RefSeqi NP_013128.1. NM_001181915.1.

3D structure databases

ProteinModelPortali P54113.
SMRi P54113. Positions 6-591.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 31302. 35 interactions.
DIPi DIP-4657N.
IntActi P54113. 16 interactions.
MINTi MINT-477775.
STRINGi 4932.YLR028C.

Proteomic databases

MaxQBi P54113.
PaxDbi P54113.
PeptideAtlasi P54113.
PRIDEi P54113.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YLR028C ; YLR028C ; YLR028C .
GeneIDi 850715.
KEGGi sce:YLR028C.

Organism-specific databases

CYGDi YLR028c.
SGDi S000004018. ADE16.

Phylogenomic databases

eggNOGi COG0138.
GeneTreei ENSGT00760000120248.
HOGENOMi HOG000230372.
InParanoidi P54113.
KOi K00602.
OMAi VMSERIP.
OrthoDBi EOG7HXD0J.

Enzyme and pathway databases

UniPathwayi UPA00074 ; UER00133 .
UPA00074 ; UER00135 .
BioCyci MetaCyc:YLR028C-MONOMER.
YEAST:YLR028C-MONOMER.

Miscellaneous databases

NextBioi 966781.

Gene expression databases

Genevestigatori P54113.

Family and domain databases

Gene3Di 1.10.287.440. 1 hit.
3.40.140.20. 3 hits.
3.40.50.1380. 1 hit.
HAMAPi MF_00139. PurH.
InterProi IPR024051. AICAR_Tfase_dom.
IPR024050. AICAR_Tfase_insert_dom.
IPR002695. AICARFT_IMPCHas.
IPR016193. Cytidine_deaminase-like.
IPR011607. MGS-like_dom.
[Graphical view ]
PANTHERi PTHR11692. PTHR11692. 1 hit.
Pfami PF01808. AICARFT_IMPCHas. 1 hit.
PF02142. MGS. 1 hit.
[Graphical view ]
PIRSFi PIRSF000414. AICARFT_IMPCHas. 1 hit.
SMARTi SM00798. AICARFT_IMPCHas. 1 hit.
SM00851. MGS. 1 hit.
[Graphical view ]
SUPFAMi SSF52335. SSF52335. 1 hit.
SSF53927. SSF53927. 1 hit.
TIGRFAMsi TIGR00355. purH. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Tibbetts A.S., Appling D.R.
    Submitted (JUN-1996) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XII."
    Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H.
    , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D.
    Nature 387:87-90(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiPUR91_YEAST
AccessioniPrimary (citable) accession number: P54113
Secondary accession number(s): D6VY30, E9PAG6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: October 29, 2014
This is version 127 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 7700 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XII
    Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

External Data

Dasty 3