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P54113

- PUR91_YEAST

UniProt

P54113 - PUR91_YEAST

Protein

Bifunctional purine biosynthesis protein ADE16

Gene

ADE16

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 126 (01 Oct 2014)
      Sequence version 1 (01 Oct 1996)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    10-formyltetrahydrofolate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide = tetrahydrofolate + 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide.
    IMP + H2O = 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide.

    Pathwayi

    GO - Molecular functioni

    1. IMP cyclohydrolase activity Source: SGD
    2. phosphoribosylaminoimidazolecarboxamide formyltransferase activity Source: SGD
    3. protein binding Source: IntAct

    GO - Biological processi

    1. 'de novo' IMP biosynthetic process Source: SGD
    2. aerobic respiration Source: SGD
    3. ascospore formation Source: SGD
    4. purine nucleotide biosynthetic process Source: SGD

    Keywords - Molecular functioni

    Hydrolase, Transferase

    Keywords - Biological processi

    Purine biosynthesis

    Enzyme and pathway databases

    BioCyciMetaCyc:YLR028C-MONOMER.
    YEAST:YLR028C-MONOMER.
    UniPathwayiUPA00074; UER00133.
    UPA00074; UER00135.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Bifunctional purine biosynthesis protein ADE16
    Including the following 2 domains:
    Phosphoribosylaminoimidazolecarboxamide formyltransferase (EC:2.1.2.3)
    Alternative name(s):
    5-aminoimidazole-4-carboxamide ribonucleotide formyltransferase
    AICAR transformylase
    IMP cyclohydrolase (EC:3.5.4.10)
    Alternative name(s):
    ATIC
    IMP synthase
    Inosinicase
    Gene namesi
    Name:ADE16
    Ordered Locus Names:YLR028C
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XII

    Organism-specific databases

    CYGDiYLR028c.
    SGDiS000004018. ADE16.

    Subcellular locationi

    GO - Cellular componenti

    1. cytosol Source: SGD

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 591591Bifunctional purine biosynthesis protein ADE16PRO_0000192159Add
    BLAST

    Proteomic databases

    MaxQBiP54113.
    PaxDbiP54113.
    PeptideAtlasiP54113.
    PRIDEiP54113.

    Expressioni

    Gene expression databases

    GenevestigatoriP54113.

    Interactioni

    Subunit structurei

    Homodimer (Possible).

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    ADE17P380093EBI-14213,EBI-14223

    Protein-protein interaction databases

    BioGridi31302. 34 interactions.
    DIPiDIP-4657N.
    IntActiP54113. 16 interactions.
    MINTiMINT-477775.
    STRINGi4932.YLR028C.

    Structurei

    3D structure databases

    ProteinModelPortaliP54113.
    SMRiP54113. Positions 6-591.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domaini

    The IMP cyclohydrolase activity resides in the N-terminal region.By similarity

    Sequence similaritiesi

    Belongs to the PurH family.Curated

    Phylogenomic databases

    eggNOGiCOG0138.
    GeneTreeiENSGT00390000004553.
    HOGENOMiHOG000230372.
    KOiK00602.
    OMAiVMSERIP.
    OrthoDBiEOG7HXD0J.

    Family and domain databases

    Gene3Di1.10.287.440. 1 hit.
    3.40.140.20. 3 hits.
    3.40.50.1380. 1 hit.
    HAMAPiMF_00139. PurH.
    InterProiIPR024051. AICAR_Tfase_dom.
    IPR024050. AICAR_Tfase_insert_dom.
    IPR002695. AICARFT_IMPCHas.
    IPR016193. Cytidine_deaminase-like.
    IPR011607. MGS-like_dom.
    [Graphical view]
    PANTHERiPTHR11692. PTHR11692. 1 hit.
    PfamiPF01808. AICARFT_IMPCHas. 1 hit.
    PF02142. MGS. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000414. AICARFT_IMPCHas. 1 hit.
    SMARTiSM00798. AICARFT_IMPCHas. 1 hit.
    SM00851. MGS. 1 hit.
    [Graphical view]
    SUPFAMiSSF52335. SSF52335. 1 hit.
    SSF53927. SSF53927. 1 hit.
    TIGRFAMsiTIGR00355. purH. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P54113-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGKYTKTAIL SVYDKTGLLD LAKGLVENNV RILASGGTAN MVREAGFPVD    50
    DVSSITHAPE MLGGRVKTLH PAVHAGILAR NLEGDEKDLK EQHIDKVDFV 100
    VCNLYPFKET VAKIGVTVQE AVEEIDIGGV TLLRAAAKNH SRVTILSDPN 150
    DYSIFLQDLS KDGEISQDLR NRFALKAFEH TADYDAAISD FFRKQYSEGK 200
    AQLPLRYGCN PHQRPAQAYI TQQEELPFKV LCGTPGYINL LDALNSWPLV 250
    KELSASLNLP AAASFKHVSP AGAAVGLPLS DVERQVYFVN DMEDLSPLAC 300
    AYARARGADR MSSFGDFIAL SNIVDVATAK IISKEVSDGV IAPGYEPEAL 350
    NILSKKKNGK YCILQIDPNY VPGQMESREV FGVTLQQKRN DAIINQSTFK 400
    EIVSKNKALT EQAVIDLTVA TLVLKYTQSN SVCYAKNGMV VGLGAGQQSR 450
    IHCTRLAGDK TDNWWLRQHP KVLNMKWAKG IKRADKSNAI DLFVTGQRIE 500
    GPEKVDYESK FEEVPEPFTK EERLEWLSKL NNVSLSSDAF FPFPDNVYRA 550
    VQSGVKFITA PSGSVMDKVV FQAADSFDIV YVENPIRLFH H 591
    Length:591
    Mass (Da):65,282
    Last modified:October 1, 1996 - v1
    Checksum:iDEC06684BFED7CA7
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U62402 Genomic DNA. Translation: AAB57774.1.
    Z73199 Genomic DNA. Translation: CAA97551.2. Sequence problems.
    Z73200 Genomic DNA. Translation: CAA97552.1.
    BK006945 Genomic DNA. Translation: DAA09346.1.
    PIRiS77707.
    RefSeqiNP_013128.1. NM_001181915.1.

    Genome annotation databases

    EnsemblFungiiYLR028C; YLR028C; YLR028C.
    GeneIDi850715.
    KEGGisce:YLR028C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U62402 Genomic DNA. Translation: AAB57774.1 .
    Z73199 Genomic DNA. Translation: CAA97551.2 . Sequence problems.
    Z73200 Genomic DNA. Translation: CAA97552.1 .
    BK006945 Genomic DNA. Translation: DAA09346.1 .
    PIRi S77707.
    RefSeqi NP_013128.1. NM_001181915.1.

    3D structure databases

    ProteinModelPortali P54113.
    SMRi P54113. Positions 6-591.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 31302. 34 interactions.
    DIPi DIP-4657N.
    IntActi P54113. 16 interactions.
    MINTi MINT-477775.
    STRINGi 4932.YLR028C.

    Proteomic databases

    MaxQBi P54113.
    PaxDbi P54113.
    PeptideAtlasi P54113.
    PRIDEi P54113.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YLR028C ; YLR028C ; YLR028C .
    GeneIDi 850715.
    KEGGi sce:YLR028C.

    Organism-specific databases

    CYGDi YLR028c.
    SGDi S000004018. ADE16.

    Phylogenomic databases

    eggNOGi COG0138.
    GeneTreei ENSGT00390000004553.
    HOGENOMi HOG000230372.
    KOi K00602.
    OMAi VMSERIP.
    OrthoDBi EOG7HXD0J.

    Enzyme and pathway databases

    UniPathwayi UPA00074 ; UER00133 .
    UPA00074 ; UER00135 .
    BioCyci MetaCyc:YLR028C-MONOMER.
    YEAST:YLR028C-MONOMER.

    Miscellaneous databases

    NextBioi 966781.

    Gene expression databases

    Genevestigatori P54113.

    Family and domain databases

    Gene3Di 1.10.287.440. 1 hit.
    3.40.140.20. 3 hits.
    3.40.50.1380. 1 hit.
    HAMAPi MF_00139. PurH.
    InterProi IPR024051. AICAR_Tfase_dom.
    IPR024050. AICAR_Tfase_insert_dom.
    IPR002695. AICARFT_IMPCHas.
    IPR016193. Cytidine_deaminase-like.
    IPR011607. MGS-like_dom.
    [Graphical view ]
    PANTHERi PTHR11692. PTHR11692. 1 hit.
    Pfami PF01808. AICARFT_IMPCHas. 1 hit.
    PF02142. MGS. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000414. AICARFT_IMPCHas. 1 hit.
    SMARTi SM00798. AICARFT_IMPCHas. 1 hit.
    SM00851. MGS. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52335. SSF52335. 1 hit.
    SSF53927. SSF53927. 1 hit.
    TIGRFAMsi TIGR00355. purH. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Tibbetts A.S., Appling D.R.
      Submitted (JUN-1996) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XII."
      Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H.
      , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D.
      Nature 387:87-90(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiPUR91_YEAST
    AccessioniPrimary (citable) accession number: P54113
    Secondary accession number(s): D6VY30, E9PAG6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 126 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 7700 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Multifunctional enzyme, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome XII
      Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

    External Data

    Dasty 3