P54108 (CRIS3_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cysteine-rich secretory protein 3
Short name=CRISP-3

Alternative name(s):
Specific granule protein of 28 kDa
Short name=SGP28

Gene names

Name:

CRISP3

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	245 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Subunit structure	Interacts with A1BG. Ref.7
Subcellular location	Secreted. Note: In neutrophils, localized in specific granules.
Tissue specificity	Salivary gland, pancreas and prostate > epididymis, ovary, thymus and colon.
Sequence similarities	Belongs to the CRISP family. Contains 1 SCP domain.
Sequence caution	The sequence AAH69580.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended. The sequence AAH69602.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended. The sequence BAG36185.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended. The sequence CAA63984.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended. The sequence CAA64527.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

Ontologies

Keywords
Cellular component	Secreted
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Signal
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	innate immune response Non-traceable author statement PubMed 12223513. Source: UniProtKB
Cellular_component	proteinaceous extracellular matrix Non-traceable author statement PubMed 12223513. Source: UniProtKB specific granule Inferred from direct assay PubMed 12223513Ref.1. Source: UniProtKB
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: P54108-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: P54108-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-1: M → MKQILHPALETTAM

Isoform 3 (identifier: P54108-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-1: M → MKQILHPALETTDPCSTGFVFPAM

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 20	20	Potential
	Chain	21 – 245	225	Cysteine-rich secretory protein 3		PRO_0000006268
Regions
	Domain	43 – 171	129	SCP
Amino acid modifications
	Glycosylation	239	1	N-linked (GlcNAc...) Potential
	Disulfide bond	191 ↔ 198		By similarity
	Disulfide bond	194 ↔ 203		By similarity
	Disulfide bond	207 ↔ 240		By similarity
	Disulfide bond	216 ↔ 234		By similarity
	Disulfide bond	225 ↔ 238		By similarity
Natural variations
	Alternative sequence	1	1	M → MKQILHPALETTAM in isoform 2.		VSP_042758
	Alternative sequence	1	1	M → MKQILHPALETTDPCSTGFV FPAM in isoform 3.		VSP_042759
	Natural variant	106	1	S → P. Ref.2 Ref.5 Corresponds to variant rs495335 [ dbSNP | Ensembl ].		VAR_011718
	Natural variant	134	1	A → S. Ref.2 Corresponds to variant rs1864312 [ dbSNP | Ensembl ].		VAR_011719
Experimental info
	Sequence conflict	79	1	Q → R in BAF85475. Ref.2
	Sequence conflict	130	1	K → R in BAD97100. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: B4DD79CB7AE9E5F9	FASTA	245	27,630
	10 20 30 40 50 60 MTLFPVLLFL VAGLLPSFPA NEDKDPAFTA LLTTQTQVQR EIVNKHNELR RAVSPPARNM 70 80 90 100 110 120 LKMEWNKEAA ANAQKWANQC NYRHSNPKDR MTSLKCGENL YMSSASSSWS QAIQSWFDEY 130 140 150 160 170 180 NDFDFGVGPK TPNAVVGHYT QVVWYSSYLV GCGNAYCPNQ KVLKYYYVCQ YCPAGNWANR 190 200 210 220 230 240 LYVPYEQGAP CASCPDNCDD GLCTNGCKYE DLYSNCKSLK LTLTCKHQLV RDSCKASCNC SNSIY
	Isoform 2 [UniParc]. Checksum: 4371D621491B8427	FASTA	258	29,065
	10 20 30 40 50 60 MKQILHPALE TTAMTLFPVL LFLVAGLLPS FPANEDKDPA FTALLTTQTQ VQREIVNKHN 70 80 90 100 110 120 ELRRAVSPPA RNMLKMEWNK EAAANAQKWA NQCNYRHSNP KDRMTSLKCG ENLYMSSASS 130 140 150 160 170 180 SWSQAIQSWF DEYNDFDFGV GPKTPNAVVG HYTQVVWYSS YLVGCGNAYC PNQKVLKYYY 190 200 210 220 230 240 VCQYCPAGNW ANRLYVPYEQ GAPCASCPDN CDDGLCTNGC KYEDLYSNCK SLKLTLTCKH 250 QLVRDSCKAS CNCSNSIY
	Isoform 3 [UniParc]. Checksum: 4DA3F68D69A9953D	FASTA	268	30,116
	10 20 30 40 50 60 MKQILHPALE TTDPCSTGFV FPAMTLFPVL LFLVAGLLPS FPANEDKDPA FTALLTTQTQ 70 80 90 100 110 120 VQREIVNKHN ELRRAVSPPA RNMLKMEWNK EAAANAQKWA NQCNYRHSNP KDRMTSLKCG 130 140 150 160 170 180 ENLYMSSASS SWSQAIQSWF DEYNDFDFGV GPKTPNAVVG HYTQVVWYSS YLVGCGNAYC 190 200 210 220 230 240 PNQKVLKYYY VCQYCPAGNW ANRLYVPYEQ GAPCASCPDN CDDGLCTNGC KYEDLYSNCK 250 260 SLKLTLTCKH QLVRDSCKAS CNCSNSIY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"SGP28, a novel matrix glycoprotein in specific granules of human neutrophils with similarity to a human testis-specific gene product and a rodent sperm-coating glycoprotein." Kjeldsen L., Cowland J.B., Johnson A.H., Borregaard N. FEBS Lett. 380:246-250(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), NUCLEOTIDE SEQUENCE [MRNA] OF 1-155 (ISOFORM 3), VARIANTS PRO-106 AND SER-134. Tissue: Trachea.
[3]	Totoki Y., Toyoda A., Takeda T., Sakaki Y., Tanaka A., Yokoyama S. Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Gastric mucosa.
[4]	"The DNA sequence and analysis of human chromosome 6." Mungall A.J., Palmer S.A., Sims S.K., Edwards C.A., Ashurst J.L., Wilming L., Jones M.C., Horton R., Hunt S.E., Scott C.E., Gilbert J.G.R., Clamp M.E., Bethel G., Milne S., Ainscough R., Almeida J.P., Ambrose K.D., Andrews T.D. , Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beare D.M., Beasley H., Beasley O., Bird C.P., Blakey S.E., Bray-Allen S., Brook J., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Clark S.Y., Clark G., Clee C.M., Clegg S., Cobley V., Collier R.E., Collins J.E., Colman L.K., Corby N.R., Coville G.J., Culley K.M., Dhami P., Davies J., Dunn M., Earthrowl M.E., Ellington A.E., Evans K.A., Faulkner L., Francis M.D., Frankish A., Frankland J., French L., Garner P., Garnett J., Ghori M.J., Gilby L.M., Gillson C.J., Glithero R.J., Grafham D.V., Grant M., Gribble S., Griffiths C., Griffiths M.N.D., Hall R., Halls K.S., Hammond S., Harley J.L., Hart E.A., Heath P.D., Heathcott R., Holmes S.J., Howden P.J., Howe K.L., Howell G.R., Huckle E., Humphray S.J., Humphries M.D., Hunt A.R., Johnson C.M., Joy A.A., Kay M., Keenan S.J., Kimberley A.M., King A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C.R., Lloyd D.M., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., Maslen G.L., Matthews L., McCann O.T., McLaren S.J., McLay K., McMurray A., Moore M.J.F., Mullikin J.C., Niblett D., Nickerson T., Novik K.L., Oliver K., Overton-Larty E.K., Parker A., Patel R., Pearce A.V., Peck A.I., Phillimore B.J.C.T., Phillips S., Plumb R.W., Porter K.M., Ramsey Y., Ranby S.A., Rice C.M., Ross M.T., Searle S.M., Sehra H.K., Sheridan E., Skuce C.D., Smith S., Smith M., Spraggon L., Squares S.L., Steward C.A., Sycamore N., Tamlyn-Hall G., Tester J., Theaker A.J., Thomas D.W., Thorpe A., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., White S.S., Whitehead S.L., Whittaker H., Wild A., Willey D.J., Wilmer T.E., Wood J.M., Wray P.W., Wyatt J.C., Young L., Younger R.M., Bentley D.R., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Dunham I., Rogers J., Beck S. Nature 425:805-811(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), PARTIAL NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), VARIANT PRO-106. Tissue: Placenta.
[6]	"The human cysteine-rich secretory protein (CRISP) family. Primary structure and tissue distribution of CRISP-1, CRISP-2 and CRISP-3." Kraetzschmar J., Haendler B., Eberspaecher U., Roostermann D., Donner P., Schleuning W.-D. Eur. J. Biochem. 236:827-836(1996) [PubMed] [Europe PMC] [Abstract] Cited for: PARTIAL NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
[7]	"Cysteine-rich secretory protein 3 is a ligand of alpha1B-glycoprotein in human plasma." Udby L., Sorensen O.E., Pass J., Johnsen A.H., Behrendt N., Borregaard N., Kjeldsen L. Biochemistry 43:12877-12886(2004) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH A1BG.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X94323 mRNA. Translation: CAA63984.1. Different initiation. AK292786 mRNA. Translation: BAF85475.1. AK313387 mRNA. Translation: BAG36185.1. Different initiation. DB205467 mRNA. No translation available. AK223380 mRNA. Translation: BAD97100.1. AL121974, AL121950 Genomic DNA. Translation: CAI20517.1. AL121950, AL121974 Genomic DNA. Translation: CAI20995.1. BC069580 mRNA. Translation: AAH69580.1. Different initiation. BC069602 mRNA. Translation: AAH69602.1. Different initiation. BC101539 mRNA. Translation: AAI01540.1. X95240 mRNA. Translation: CAA64527.1. Different initiation.
IPI	IPI00942117.
PIR	S68691.
RefSeq	NP_001177915.1. NM_001190986.1. NP_006052.2. NM_006061.2.
UniGene	Hs.404466.
3D structure databases
ProteinModelPortal	P54108.
ModBase	Search...
Protein-protein interaction databases
STRING	9606.ENSP00000263045.
PTM databases
PhosphoSite	P54108.
Polymorphism databases
DMDM	1706135.
Proteomic databases
PaxDb	P54108.
PeptideAtlas	P54108.
PRIDE	P54108.
Protocols and materials databases
DNASU	10321.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000263045; ENSP00000263045; ENSG00000096006. ENST00000393666; ENSP00000377274; ENSG00000096006. ENST00000433368; ENSP00000389026; ENSG00000096006.
GeneID	10321.
KEGG	hsa:10321.
UCSC	uc003ozs.3. human.
Organism-specific databases
CTD	10321.
GeneCards	GC06M049741.
H-InvDB	HIX0032937.
HGNC	HGNC:16904. CRISP3.
neXtProt	NX_P54108.
PharmGKB	PA134880440.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG312931.
HOGENOM	HOG000236338.
HOVERGEN	HBG004184.
InParanoid	P54108.
OrthoDB	EOG4XKV7K.
PhylomeDB	P54108.
Gene expression databases
ArrayExpress	P54108.
Bgee	P54108.
CleanEx	HS_CRISP3.
Genevestigator	P54108.
GermOnline	ENSG00000096006. Homo sapiens.
Family and domain databases
Gene3D	3.40.33.10. 1 hit.
InterPro	IPR001283. Allrgn_V5/Tpx1. IPR018244. Allrgn_V5/Tpx1_CS. IPR014044. CAP_domain. IPR013871. Cysteine_rich_secretory. [Graphical view]
PANTHER	PTHR10334. PTHR10334. 1 hit.
Pfam	PF00188. CAP. 1 hit. PF08562. Crisp. 1 hit. [Graphical view]
PRINTS	PR00837. V5TPXLIKE.
SMART	SM00198. SCP. 1 hit. [Graphical view]
SUPFAM	SSF55797. SCP-like_extracellular. 1 hit.
PROSITE	PS01009. CRISP_1. 1 hit. PS01010. CRISP_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	10321.
NextBio	39127.

Entry information

Entry name

CRIS3_HUMAN

Accession

Primary (citable) accession number: P54108
Secondary accession number(s): A8K9S1 , B2R8I8, Q15512, Q3MJ82, Q53FA9, Q5JW83, Q9H108

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	May 1, 2013
This is version 115 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.