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Protein

Cysteine-rich secretory protein 3

Gene

CRISP3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

GO - Biological processi

  1. defense response Source: UniProtKB
  2. innate immune response Source: UniProtKB
Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Cysteine-rich secretory protein 3
Short name:
CRISP-3
Alternative name(s):
Specific granule protein of 28 kDa
Short name:
SGP28
Gene namesi
Name:CRISP3
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 6

Organism-specific databases

HGNCiHGNC:16904. CRISP3.

Subcellular locationi

  1. Secreted

  2. Note: In neutrophils, localized in specific granules.

GO - Cellular componenti

  1. extracellular region Source: UniProtKB
  2. extracellular space Source: UniProtKB
  3. extracellular vesicular exosome Source: UniProtKB
  4. proteinaceous extracellular matrix Source: UniProtKB
  5. specific granule Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA134880440.

Polymorphism and mutation databases

BioMutaiCRISP3.
DMDMi1706135.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2020Sequence AnalysisAdd
BLAST
Chaini21 – 245225Cysteine-rich secretory protein 3PRO_0000006268Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi191 ↔ 198PROSITE-ProRule annotation
Disulfide bondi194 ↔ 203PROSITE-ProRule annotation
Disulfide bondi207 ↔ 240PROSITE-ProRule annotation
Disulfide bondi216 ↔ 234PROSITE-ProRule annotation
Disulfide bondi225 ↔ 238PROSITE-ProRule annotation
Glycosylationi239 – 2391N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiP54108.
PeptideAtlasiP54108.
PRIDEiP54108.

PTM databases

PhosphoSiteiP54108.

Expressioni

Tissue specificityi

Salivary gland, pancreas and prostate > epididymis, ovary, thymus and colon.

Gene expression databases

BgeeiP54108.
CleanExiHS_CRISP3.
ExpressionAtlasiP54108. baseline and differential.
GenevestigatoriP54108.

Organism-specific databases

HPAiHPA054392.

Interactioni

Subunit structurei

Interacts with A1BG.1 Publication

Protein-protein interaction databases

BioGridi115605. 1 interaction.
STRINGi9606.ENSP00000263045.

Structurei

3D structure databases

ProteinModelPortaliP54108.
SMRiP54108. Positions 39-245.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini43 – 171129SCPAdd
BLAST
Domaini207 – 24034ShKTPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the CRISP family.Curated
Contains 1 SCP domain.Curated
Contains 1 ShKT domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG312931.
GeneTreeiENSGT00760000119004.
HOGENOMiHOG000236338.
HOVERGENiHBG004184.
InParanoidiP54108.
OrthoDBiEOG7F7WB4.
PhylomeDBiP54108.
TreeFamiTF316148.

Family and domain databases

Gene3Di3.40.33.10. 1 hit.
InterProiIPR001283. Allrgn_V5/Tpx1.
IPR018244. Allrgn_V5/Tpx1_CS.
IPR014044. CAP_domain.
IPR013871. Cysteine_rich_secretory.
IPR003582. ShKT_dom.
[Graphical view]
PANTHERiPTHR10334. PTHR10334. 1 hit.
PfamiPF00188. CAP. 1 hit.
PF08562. Crisp. 1 hit.
[Graphical view]
PRINTSiPR00837. V5TPXLIKE.
SMARTiSM00198. SCP. 1 hit.
[Graphical view]
SUPFAMiSSF55797. SSF55797. 1 hit.
PROSITEiPS01009. CRISP_1. 1 hit.
PS01010. CRISP_2. 1 hit.
PS51670. SHKT. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P54108-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MTLFPVLLFL VAGLLPSFPA NEDKDPAFTA LLTTQTQVQR EIVNKHNELR
60 70 80 90 100
RAVSPPARNM LKMEWNKEAA ANAQKWANQC NYRHSNPKDR MTSLKCGENL
110 120 130 140 150
YMSSASSSWS QAIQSWFDEY NDFDFGVGPK TPNAVVGHYT QVVWYSSYLV
160 170 180 190 200
GCGNAYCPNQ KVLKYYYVCQ YCPAGNWANR LYVPYEQGAP CASCPDNCDD
210 220 230 240
GLCTNGCKYE DLYSNCKSLK LTLTCKHQLV RDSCKASCNC SNSIY
Length:245
Mass (Da):27,630
Last modified:October 1, 1996 - v1
Checksum:iB4DD79CB7AE9E5F9
GO
Isoform 2 (identifier: P54108-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-1: M → MKQILHPALETTAM

Show »
Length:258
Mass (Da):29,065
Checksum:i4371D621491B8427
GO
Isoform 3 (identifier: P54108-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-1: M → MKQILHPALETTDPCSTGFVFPAM

Show »
Length:268
Mass (Da):30,116
Checksum:i4DA3F68D69A9953D
GO

Sequence cautioni

The sequence AAH69580.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence AAH69602.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence BAG36185.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence CAA63984.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence CAA64527.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti79 – 791Q → R in BAF85475 (PubMed:14702039).Curated
Sequence conflicti130 – 1301K → R in BAD97100 (Ref. 3) Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti106 – 1061S → P.2 Publications
Corresponds to variant rs495335 [ dbSNP | Ensembl ].
VAR_011718
Natural varianti134 – 1341A → S.1 Publication
Corresponds to variant rs1864312 [ dbSNP | Ensembl ].
VAR_011719

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 11M → MKQILHPALETTAM in isoform 2. 2 PublicationsVSP_042758
Alternative sequencei1 – 11M → MKQILHPALETTDPCSTGFV FPAM in isoform 3. 1 PublicationVSP_042759

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X94323 mRNA. Translation: CAA63984.1. Different initiation.
AK292786 mRNA. Translation: BAF85475.1.
AK313387 mRNA. Translation: BAG36185.1. Different initiation.
DB205467 mRNA. No translation available.
AK223380 mRNA. Translation: BAD97100.1.
AL121974, AL121950 Genomic DNA. Translation: CAI20517.1.
AL121950, AL121974 Genomic DNA. Translation: CAI20995.1.
BC069580 mRNA. Translation: AAH69580.1. Different initiation.
BC069602 mRNA. Translation: AAH69602.1. Different initiation.
BC101539 mRNA. Translation: AAI01540.1.
X95240 mRNA. Translation: CAA64527.1. Different initiation.
CCDSiCCDS4929.2. [P54108-2]
CCDS55019.1. [P54108-3]
PIRiS68691.
RefSeqiNP_001177915.1. NM_001190986.1. [P54108-3]
NP_006052.2. NM_006061.2. [P54108-2]
UniGeneiHs.404466.

Genome annotation databases

EnsembliENST00000263045; ENSP00000263045; ENSG00000096006. [P54108-2]
ENST00000433368; ENSP00000389026; ENSG00000096006. [P54108-3]
GeneIDi10321.
KEGGihsa:10321.
UCSCiuc003ozs.3. human. [P54108-2]
uc021zai.1. human. [P54108-3]

Polymorphism and mutation databases

BioMutaiCRISP3.

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X94323 mRNA. Translation: CAA63984.1. Different initiation.
AK292786 mRNA. Translation: BAF85475.1.
AK313387 mRNA. Translation: BAG36185.1. Different initiation.
DB205467 mRNA. No translation available.
AK223380 mRNA. Translation: BAD97100.1.
AL121974, AL121950 Genomic DNA. Translation: CAI20517.1.
AL121950, AL121974 Genomic DNA. Translation: CAI20995.1.
BC069580 mRNA. Translation: AAH69580.1. Different initiation.
BC069602 mRNA. Translation: AAH69602.1. Different initiation.
BC101539 mRNA. Translation: AAI01540.1.
X95240 mRNA. Translation: CAA64527.1. Different initiation.
CCDSiCCDS4929.2. [P54108-2]
CCDS55019.1. [P54108-3]
PIRiS68691.
RefSeqiNP_001177915.1. NM_001190986.1. [P54108-3]
NP_006052.2. NM_006061.2. [P54108-2]
UniGeneiHs.404466.

3D structure databases

ProteinModelPortaliP54108.
SMRiP54108. Positions 39-245.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi115605. 1 interaction.
STRINGi9606.ENSP00000263045.

PTM databases

PhosphoSiteiP54108.

Polymorphism and mutation databases

BioMutaiCRISP3.
DMDMi1706135.

Proteomic databases

PaxDbiP54108.
PeptideAtlasiP54108.
PRIDEiP54108.

Protocols and materials databases

DNASUi10321.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000263045; ENSP00000263045; ENSG00000096006. [P54108-2]
ENST00000433368; ENSP00000389026; ENSG00000096006. [P54108-3]
GeneIDi10321.
KEGGihsa:10321.
UCSCiuc003ozs.3. human. [P54108-2]
uc021zai.1. human. [P54108-3]

Organism-specific databases

CTDi10321.
GeneCardsiGC06M049741.
H-InvDBHIX0032937.
HGNCiHGNC:16904. CRISP3.
HPAiHPA054392.
neXtProtiNX_P54108.
PharmGKBiPA134880440.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG312931.
GeneTreeiENSGT00760000119004.
HOGENOMiHOG000236338.
HOVERGENiHBG004184.
InParanoidiP54108.
OrthoDBiEOG7F7WB4.
PhylomeDBiP54108.
TreeFamiTF316148.

Miscellaneous databases

GeneWikiiCRISP3.
GenomeRNAii10321.
NextBioi39127.
PROiP54108.

Gene expression databases

BgeeiP54108.
CleanExiHS_CRISP3.
ExpressionAtlasiP54108. baseline and differential.
GenevestigatoriP54108.

Family and domain databases

Gene3Di3.40.33.10. 1 hit.
InterProiIPR001283. Allrgn_V5/Tpx1.
IPR018244. Allrgn_V5/Tpx1_CS.
IPR014044. CAP_domain.
IPR013871. Cysteine_rich_secretory.
IPR003582. ShKT_dom.
[Graphical view]
PANTHERiPTHR10334. PTHR10334. 1 hit.
PfamiPF00188. CAP. 1 hit.
PF08562. Crisp. 1 hit.
[Graphical view]
PRINTSiPR00837. V5TPXLIKE.
SMARTiSM00198. SCP. 1 hit.
[Graphical view]
SUPFAMiSSF55797. SSF55797. 1 hit.
PROSITEiPS01009. CRISP_1. 1 hit.
PS01010. CRISP_2. 1 hit.
PS51670. SHKT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "SGP28, a novel matrix glycoprotein in specific granules of human neutrophils with similarity to a human testis-specific gene product and a rodent sperm-coating glycoprotein."
    Kjeldsen L., Cowland J.B., Johnson A.H., Borregaard N.
    FEBS Lett. 380:246-250(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
  2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), NUCLEOTIDE SEQUENCE [MRNA] OF 1-155 (ISOFORM 3), VARIANTS PRO-106 AND SER-134.
    Tissue: Trachea.
  3. Totoki Y., Toyoda A., Takeda T., Sakaki Y., Tanaka A., Yokoyama S.
    Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Gastric mucosa.
  4. "The DNA sequence and analysis of human chromosome 6."
    Mungall A.J., Palmer S.A., Sims S.K., Edwards C.A., Ashurst J.L., Wilming L., Jones M.C., Horton R., Hunt S.E., Scott C.E., Gilbert J.G.R., Clamp M.E., Bethel G., Milne S., Ainscough R., Almeida J.P., Ambrose K.D., Andrews T.D.
    , Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beare D.M., Beasley H., Beasley O., Bird C.P., Blakey S.E., Bray-Allen S., Brook J., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Clark S.Y., Clark G., Clee C.M., Clegg S., Cobley V., Collier R.E., Collins J.E., Colman L.K., Corby N.R., Coville G.J., Culley K.M., Dhami P., Davies J., Dunn M., Earthrowl M.E., Ellington A.E., Evans K.A., Faulkner L., Francis M.D., Frankish A., Frankland J., French L., Garner P., Garnett J., Ghori M.J., Gilby L.M., Gillson C.J., Glithero R.J., Grafham D.V., Grant M., Gribble S., Griffiths C., Griffiths M.N.D., Hall R., Halls K.S., Hammond S., Harley J.L., Hart E.A., Heath P.D., Heathcott R., Holmes S.J., Howden P.J., Howe K.L., Howell G.R., Huckle E., Humphray S.J., Humphries M.D., Hunt A.R., Johnson C.M., Joy A.A., Kay M., Keenan S.J., Kimberley A.M., King A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C.R., Lloyd D.M., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., Maslen G.L., Matthews L., McCann O.T., McLaren S.J., McLay K., McMurray A., Moore M.J.F., Mullikin J.C., Niblett D., Nickerson T., Novik K.L., Oliver K., Overton-Larty E.K., Parker A., Patel R., Pearce A.V., Peck A.I., Phillimore B.J.C.T., Phillips S., Plumb R.W., Porter K.M., Ramsey Y., Ranby S.A., Rice C.M., Ross M.T., Searle S.M., Sehra H.K., Sheridan E., Skuce C.D., Smith S., Smith M., Spraggon L., Squares S.L., Steward C.A., Sycamore N., Tamlyn-Hall G., Tester J., Theaker A.J., Thomas D.W., Thorpe A., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., White S.S., Whitehead S.L., Whittaker H., Wild A., Willey D.J., Wilmer T.E., Wood J.M., Wray P.W., Wyatt J.C., Young L., Younger R.M., Bentley D.R., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Dunham I., Rogers J., Beck S.
    Nature 425:805-811(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), PARTIAL NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), VARIANT PRO-106.
    Tissue: Placenta.
  6. "The human cysteine-rich secretory protein (CRISP) family. Primary structure and tissue distribution of CRISP-1, CRISP-2 and CRISP-3."
    Kraetzschmar J., Haendler B., Eberspaecher U., Roostermann D., Donner P., Schleuning W.-D.
    Eur. J. Biochem. 236:827-836(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: PARTIAL NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
  7. "Cysteine-rich secretory protein 3 is a ligand of alpha1B-glycoprotein in human plasma."
    Udby L., Sorensen O.E., Pass J., Johnsen A.H., Behrendt N., Borregaard N., Kjeldsen L.
    Biochemistry 43:12877-12886(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH A1BG.

Entry informationi

Entry nameiCRIS3_HUMAN
AccessioniPrimary (citable) accession number: P54108
Secondary accession number(s): A8K9S1
, B2R8I8, Q15512, Q3MJ82, Q53FA9, Q5JW83, Q9H108
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: April 29, 2015
This is version 131 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 6
    Human chromosome 6: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.