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Protein

DnaJ homolog subfamily C member 2

Gene

Dnajc2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Acts both as a chaperone in the cytosol and as a chromatin regulator in the nucleus. When cytosolic, acts as a molecular chaperone: component of the ribosome-associated complex (RAC), a complex involved in folding or maintaining nascent polypeptides in a folding-competent state. In the RAC complex, stimulates the ATPase activity of the ribosome-associated pool of Hsp70-type chaperones HSPA14 that bind to the nascent polypeptide chain. When nuclear, mediates the switching from polycomb-repressed genes to an active state: specifically recruited at histone H2A ubiquitinated at 'Lys-119' (H2AK119ub), and promotes the displacement of the polycomb PRC1 complex from chromatin, thereby facilitating transcription activation (By similarity). Specifically binds DNA sequence 5'-GTCAAGC-3'.By similarity

GO - Molecular functioni

GO - Biological processi

  • chromatin modification Source: UniProtKB-KW
  • DNA replication Source: MGI
  • negative regulation of cell growth Source: Ensembl
  • negative regulation of DNA biosynthetic process Source: MGI
  • positive regulation of transcription, DNA-templated Source: UniProtKB
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator, Chaperone, Chromatin regulator

Keywords - Biological processi

Transcription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-MMU-3371453. Regulation of HSF1-mediated heat shock response.

Names & Taxonomyi

Protein namesi
Recommended name:
DnaJ homolog subfamily C member 2
Alternative name(s):
Mouse Id associate 1
Short name:
MIDA1
Zuotin-related factor 1
Cleaved into the following chain:
Gene namesi
Name:Dnajc2
Synonyms:Mida1, Zrf1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:99470. Dnajc2.

Subcellular locationi

  • Nucleus PROSITE-ProRule annotation1 Publication
  • Cytoplasmcytosol By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 621621DnaJ homolog subfamily C member 2PRO_0000071124Add
BLAST
Initiator methionineiRemoved; alternateBy similarity
Chaini2 – 621620DnaJ homolog subfamily C member 2, N-terminally processedPRO_0000434352Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionineBy similarity
Modified residuei47 – 471PhosphoserineCombined sources
Modified residuei49 – 491PhosphoserineCombined sources
Modified residuei60 – 601PhosphoserineCombined sources
Modified residuei63 – 631PhosphoserineCombined sources
Modified residuei183 – 1831PhosphoserineCombined sources

Post-translational modificationi

Phosphorylated in M (mitotic) phase.By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiP54103.
MaxQBiP54103.
PaxDbiP54103.
PeptideAtlasiP54103.
PRIDEiP54103.

PTM databases

iPTMnetiP54103.
PhosphoSiteiP54103.

Expressioni

Tissue specificityi

Expressed in all tissues.1 Publication

Gene expression databases

BgeeiENSMUSG00000029014.
CleanExiMM_DNAJC2.
ExpressionAtlasiP54103. baseline and differential.
GenevisibleiP54103. MM.

Interactioni

Subunit structurei

Component of ribosome-associated complex (RAC), a heterodimer composed of Hsp70/DnaK-type chaperone HSPA14 and Hsp40/DnaJ-type chaperone DNAJC2 (By similarity). Interacts (via ZRF1-UBD region) with ID1.By similarity2 Publications

GO - Molecular functioni

Protein-protein interaction databases

BioGridi204711. 2 interactions.
IntActiP54103. 1 interaction.
MINTiMINT-4373956.
STRINGi10090.ENSMUSP00000030771.

Structurei

3D structure databases

ProteinModelPortaliP54103.
SMRiP54103. Positions 87-163, 552-620.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini88 – 16174JPROSITE-ProRule annotationAdd
BLAST
Domaini449 – 51163SANT 1PROSITE-ProRule annotationAdd
BLAST
Domaini549 – 60456SANT 2PROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni160 – 25091ZRF1-UBDAdd
BLAST

Domaini

The ZRF1-UBD region specifically recognizes and binds H2AK119ub. The ZRF1-UBD region is also involved in protein-protein interactions with other proteins, suggesting that it may be masked by some regulator, thereby preventing its association with H2AK119ub (By similarity).By similarity

Sequence similaritiesi

Contains 1 J domain.PROSITE-ProRule annotation
Contains 2 SANT domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0724. Eukaryota.
COG5269. LUCA.
GeneTreeiENSGT00530000063419.
HOGENOMiHOG000006900.
HOVERGENiHBG008782.
InParanoidiP54103.
KOiK09522.
OMAiLTSCTKE.
OrthoDBiEOG091G04S8.
PhylomeDBiP54103.
TreeFamiTF105834.

Family and domain databases

CDDicd06257. DnaJ. 1 hit.
Gene3Di1.10.10.60. 1 hit.
1.10.287.110. 1 hit.
InterProiIPR001623. DnaJ_domain.
IPR018253. DnaJ_domain_CS.
IPR009057. Homeodomain-like.
IPR017930. Myb_dom.
IPR032003. RAC_head.
IPR001005. SANT/Myb.
IPR017884. SANT_dom.
[Graphical view]
PfamiPF00226. DnaJ. 1 hit.
PF00249. Myb_DNA-binding. 2 hits.
PF16717. RAC_head. 1 hit.
[Graphical view]
SMARTiSM00271. DnaJ. 1 hit.
SM00717. SANT. 2 hits.
[Graphical view]
SUPFAMiSSF46565. SSF46565. 1 hit.
SSF46689. SSF46689. 2 hits.
PROSITEiPS00636. DNAJ_1. 1 hit.
PS50076. DNAJ_2. 1 hit.
PS51293. SANT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P54103-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLLLPSAAEG QGTAITHALT SASSVCQVEP VGRWFEAFVK RRNRNASTSF
60 70 80 90 100
QELEDKKELS EESEDEELQL EEFPMLKTLD PKDWKNQDHY AVLGLGHVRY
110 120 130 140 150
TATQRQIKAA HKAMVLKHHP DKRKAAGEPI KEGDNDYFTC ITKAYEMLSD
160 170 180 190 200
PVKRRAFNSV DPTFDNSVPS KSEAKDNFFQ VFSPVFERNS RWSNKKNVPK
210 220 230 240 250
LGDMNSSFED VDAFYSFWYN FDSWREFSYL DEEEKEKAEC RDERKWIEKQ
260 270 280 290 300
NRATRAQRKK EEMNRIRTLV DNAYSCDPRI KKFKEEEKAK KEAEKKAKAE
310 320 330 340 350
ARRKEQEAKE KQRQAELEAV RLAKEKEEEE VRQQALLAKK EKDIQKKAIK
360 370 380 390 400
KERQKLRNSC KSWNHFSDNE ADRVKMMEEV EKLCDRLELA SLQGLNEILA
410 420 430 440 450
SSTREVGKAA LEKQIEEVNE QMRREKEEAD ARMRQASKNA EKSTGGSGSG
460 470 480 490 500
SKNWSEDDLQ LLIKAVNLFP AGTNSRWEVI ANYMNIHSSS GVKRTAKDVI
510 520 530 540 550
SKAKSLQKLD PHQKDDINKK AFDKFKKEHG VASQADSAAP SERFEGPCID
560 570 580 590 600
STPWTTEEQK LLEQALKTYP VNTPERWEKI AEAVPGRTKK DCMRRYKELV
610 620
EMVKAKKAAQ EQVLNASRAR K
Length:621
Mass (Da):71,722
Last modified:March 6, 2007 - v2
Checksum:i0FFC511028EA1C41
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti473 – 4731T → R in AAC52486 (PubMed:8666407).Curated
Sequence conflicti478 – 4781E → D in AAC52486 (PubMed:8666407).Curated
Sequence conflicti502 – 51413KAKSL…DPHQK → EVRGPKSWGSSKR in AAC52486 (PubMed:8666407).CuratedAdd
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D63784 mRNA. Translation: BAA09854.1.
AK131964 mRNA. Translation: BAE20907.1.
BC052027 mRNA. Translation: AAH52027.1.
U53208 mRNA. Translation: AAC52486.1.
CCDSiCCDS19107.1.
PIRiA57591.
RefSeqiNP_033610.1. NM_009584.4.
UniGeneiMm.266312.

Genome annotation databases

EnsembliENSMUST00000030771; ENSMUSP00000030771; ENSMUSG00000029014.
GeneIDi22791.
KEGGimmu:22791.
UCSCiuc008wpa.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D63784 mRNA. Translation: BAA09854.1.
AK131964 mRNA. Translation: BAE20907.1.
BC052027 mRNA. Translation: AAH52027.1.
U53208 mRNA. Translation: AAC52486.1.
CCDSiCCDS19107.1.
PIRiA57591.
RefSeqiNP_033610.1. NM_009584.4.
UniGeneiMm.266312.

3D structure databases

ProteinModelPortaliP54103.
SMRiP54103. Positions 87-163, 552-620.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi204711. 2 interactions.
IntActiP54103. 1 interaction.
MINTiMINT-4373956.
STRINGi10090.ENSMUSP00000030771.

PTM databases

iPTMnetiP54103.
PhosphoSiteiP54103.

Proteomic databases

EPDiP54103.
MaxQBiP54103.
PaxDbiP54103.
PeptideAtlasiP54103.
PRIDEiP54103.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000030771; ENSMUSP00000030771; ENSMUSG00000029014.
GeneIDi22791.
KEGGimmu:22791.
UCSCiuc008wpa.1. mouse.

Organism-specific databases

CTDi27000.
MGIiMGI:99470. Dnajc2.

Phylogenomic databases

eggNOGiKOG0724. Eukaryota.
COG5269. LUCA.
GeneTreeiENSGT00530000063419.
HOGENOMiHOG000006900.
HOVERGENiHBG008782.
InParanoidiP54103.
KOiK09522.
OMAiLTSCTKE.
OrthoDBiEOG091G04S8.
PhylomeDBiP54103.
TreeFamiTF105834.

Enzyme and pathway databases

ReactomeiR-MMU-3371453. Regulation of HSF1-mediated heat shock response.

Miscellaneous databases

PROiP54103.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000029014.
CleanExiMM_DNAJC2.
ExpressionAtlasiP54103. baseline and differential.
GenevisibleiP54103. MM.

Family and domain databases

CDDicd06257. DnaJ. 1 hit.
Gene3Di1.10.10.60. 1 hit.
1.10.287.110. 1 hit.
InterProiIPR001623. DnaJ_domain.
IPR018253. DnaJ_domain_CS.
IPR009057. Homeodomain-like.
IPR017930. Myb_dom.
IPR032003. RAC_head.
IPR001005. SANT/Myb.
IPR017884. SANT_dom.
[Graphical view]
PfamiPF00226. DnaJ. 1 hit.
PF00249. Myb_DNA-binding. 2 hits.
PF16717. RAC_head. 1 hit.
[Graphical view]
SMARTiSM00271. DnaJ. 1 hit.
SM00717. SANT. 2 hits.
[Graphical view]
SUPFAMiSSF46565. SSF46565. 1 hit.
SSF46689. SSF46689. 2 hits.
PROSITEiPS00636. DNAJ_1. 1 hit.
PS50076. DNAJ_2. 1 hit.
PS51293. SANT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDNJC2_MOUSE
AccessioniPrimary (citable) accession number: P54103
Secondary accession number(s): Q61866
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: March 6, 2007
Last modified: September 7, 2016
This is version 129 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.