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Protein

DnaJ homolog subfamily C member 2

Gene

Dnajc2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Acts both as a chaperone in the cytosol and as a chromatin regulator in the nucleus. When cytosolic, acts as a molecular chaperone: component of the ribosome-associated complex (RAC), a complex involved in folding or maintaining nascent polypeptides in a folding-competent state. In the RAC complex, stimulates the ATPase activity of the ribosome-associated pool of Hsp70-type chaperones HSPA14 that bind to the nascent polypeptide chain. When nuclear, mediates the switching from polycomb-repressed genes to an active state: specifically recruited at histone H2A ubiquitinated at 'Lys-119' (H2AK119ub), and promotes the displacement of the polycomb PRC1 complex from chromatin, thereby facilitating transcription activation (By similarity). Specifically binds DNA sequence 5'-GTCAAGC-3'.By similarity

GO - Molecular functioni

GO - Biological processi

  • chromatin organization Source: UniProtKB-KW
  • DNA replication Source: MGI
  • negative regulation of cell growth Source: MGI
  • negative regulation of DNA biosynthetic process Source: MGI
  • positive regulation of transcription, DNA-templated Source: UniProtKB
  • transcription, DNA-templated Source: UniProtKB-KW

Keywordsi

Molecular functionActivator, Chaperone, Chromatin regulator
Biological processTranscription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-MMU-3371453 Regulation of HSF1-mediated heat shock response

Names & Taxonomyi

Protein namesi
Recommended name:
DnaJ homolog subfamily C member 2
Alternative name(s):
Mouse Id associate 1
Short name:
MIDA1
Zuotin-related factor 1
Gene namesi
Name:Dnajc2
Synonyms:Mida1, Zrf1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:99470 Dnajc2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000711241 – 621DnaJ homolog subfamily C member 2Add BLAST621

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei47PhosphoserineCombined sources1
Modified residuei49PhosphoserineCombined sources1
Modified residuei60PhosphoserineCombined sources1
Modified residuei63PhosphoserineCombined sources1
Modified residuei183PhosphoserineCombined sources1

Post-translational modificationi

Phosphorylated in M (mitotic) phase.By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiP54103
MaxQBiP54103
PaxDbiP54103
PeptideAtlasiP54103
PRIDEiP54103

PTM databases

iPTMnetiP54103
PhosphoSitePlusiP54103

Expressioni

Tissue specificityi

Expressed in all tissues.1 Publication

Gene expression databases

BgeeiENSMUSG00000029014
CleanExiMM_DNAJC2
ExpressionAtlasiP54103 baseline and differential
GenevisibleiP54103 MM

Interactioni

Subunit structurei

Component of ribosome-associated complex (RAC), a heterodimer composed of Hsp70/DnaK-type chaperone HSPA14 and Hsp40/DnaJ-type chaperone DNAJC2 (By similarity). Interacts (via ZRF1-UBD region) with ID1.By similarity2 Publications

GO - Molecular functioni

Protein-protein interaction databases

BioGridi204711, 2 interactors
IntActiP54103, 1 interactor
MINTiP54103
STRINGi10090.ENSMUSP00000030771

Structurei

3D structure databases

ProteinModelPortaliP54103
SMRiP54103
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini88 – 161JPROSITE-ProRule annotationAdd BLAST74
Domaini449 – 511SANT 1PROSITE-ProRule annotationAdd BLAST63
Domaini549 – 604SANT 2PROSITE-ProRule annotationAdd BLAST56

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni160 – 250ZRF1-UBDAdd BLAST91

Domaini

The ZRF1-UBD region specifically recognizes and binds H2AK119ub. The ZRF1-UBD region is also involved in protein-protein interactions with other proteins, suggesting that it may be masked by some regulator, thereby preventing its association with H2AK119ub (By similarity).By similarity

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0724 Eukaryota
COG5269 LUCA
GeneTreeiENSGT00530000063419
HOGENOMiHOG000006900
HOVERGENiHBG008782
InParanoidiP54103
KOiK09522
OMAiTTPDRWD
OrthoDBiEOG091G04S8
PhylomeDBiP54103
TreeFamiTF105834

Family and domain databases

CDDicd06257 DnaJ, 1 hit
cd00167 SANT, 2 hits
Gene3Di1.10.287.110, 1 hit
InterProiView protein in InterPro
IPR001623 DnaJ_domain
IPR018253 DnaJ_domain_CS
IPR009057 Homeobox-like_sf
IPR036869 J_dom_sf
IPR017930 Myb_dom
IPR032003 RAC_head
IPR001005 SANT/Myb
IPR017884 SANT_dom
PfamiView protein in Pfam
PF00226 DnaJ, 1 hit
PF00249 Myb_DNA-binding, 2 hits
PF16717 RAC_head, 1 hit
SMARTiView protein in SMART
SM00271 DnaJ, 1 hit
SM00717 SANT, 2 hits
SUPFAMiSSF46565 SSF46565, 1 hit
SSF46689 SSF46689, 2 hits
PROSITEiView protein in PROSITE
PS00636 DNAJ_1, 1 hit
PS50076 DNAJ_2, 1 hit
PS51293 SANT, 1 hit

Sequencei

Sequence statusi: Complete.

P54103-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLLLPSAAEG QGTAITHALT SASSVCQVEP VGRWFEAFVK RRNRNASTSF
60 70 80 90 100
QELEDKKELS EESEDEELQL EEFPMLKTLD PKDWKNQDHY AVLGLGHVRY
110 120 130 140 150
TATQRQIKAA HKAMVLKHHP DKRKAAGEPI KEGDNDYFTC ITKAYEMLSD
160 170 180 190 200
PVKRRAFNSV DPTFDNSVPS KSEAKDNFFQ VFSPVFERNS RWSNKKNVPK
210 220 230 240 250
LGDMNSSFED VDAFYSFWYN FDSWREFSYL DEEEKEKAEC RDERKWIEKQ
260 270 280 290 300
NRATRAQRKK EEMNRIRTLV DNAYSCDPRI KKFKEEEKAK KEAEKKAKAE
310 320 330 340 350
ARRKEQEAKE KQRQAELEAV RLAKEKEEEE VRQQALLAKK EKDIQKKAIK
360 370 380 390 400
KERQKLRNSC KSWNHFSDNE ADRVKMMEEV EKLCDRLELA SLQGLNEILA
410 420 430 440 450
SSTREVGKAA LEKQIEEVNE QMRREKEEAD ARMRQASKNA EKSTGGSGSG
460 470 480 490 500
SKNWSEDDLQ LLIKAVNLFP AGTNSRWEVI ANYMNIHSSS GVKRTAKDVI
510 520 530 540 550
SKAKSLQKLD PHQKDDINKK AFDKFKKEHG VASQADSAAP SERFEGPCID
560 570 580 590 600
STPWTTEEQK LLEQALKTYP VNTPERWEKI AEAVPGRTKK DCMRRYKELV
610 620
EMVKAKKAAQ EQVLNASRAR K
Length:621
Mass (Da):71,722
Last modified:March 6, 2007 - v2
Checksum:i0FFC511028EA1C41
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti473T → R in AAC52486 (PubMed:8666407).Curated1
Sequence conflicti478E → D in AAC52486 (PubMed:8666407).Curated1
Sequence conflicti502 – 514KAKSL…DPHQK → EVRGPKSWGSSKR in AAC52486 (PubMed:8666407).CuratedAdd BLAST13

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D63784 mRNA Translation: BAA09854.1
AK131964 mRNA Translation: BAE20907.1
BC052027 mRNA Translation: AAH52027.1
U53208 mRNA Translation: AAC52486.1
CCDSiCCDS19107.1
PIRiA57591
RefSeqiNP_033610.1, NM_009584.4
UniGeneiMm.266312

Genome annotation databases

EnsembliENSMUST00000030771; ENSMUSP00000030771; ENSMUSG00000029014
GeneIDi22791
KEGGimmu:22791
UCSCiuc008wpa.1 mouse

Similar proteinsi

Entry informationi

Entry nameiDNJC2_MOUSE
AccessioniPrimary (citable) accession number: P54103
Secondary accession number(s): Q61866
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: March 6, 2007
Last modified: June 20, 2018
This is version 144 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

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