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Protein

DNA polymerase subunit gamma-1

Gene

Polg

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the replication of mitochondrial DNA. Associates with mitochondrial DNA (By similarity).By similarity

Catalytic activityi

Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).

Cofactori

Mg2+By similarity

GO - Molecular functioni

GO - Biological processi

  • aging Source: MGI
  • mitochondrial DNA replication Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

DNA-directed DNA polymerase, Nucleotidyltransferase, Transferase

Keywords - Biological processi

DNA replication

Keywords - Ligandi

DNA-binding, Magnesium

Names & Taxonomyi

Protein namesi
Recommended name:
DNA polymerase subunit gamma-1 (EC:2.7.7.7)
Alternative name(s):
Mitochondrial DNA polymerase catalytic subunit
PolG-alpha
Gene namesi
Name:Polg
Synonyms:Polg1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:1196389. Polg.

Subcellular locationi

GO - Cellular componenti

  • extracellular exosome Source: MGI
  • gamma DNA polymerase complex Source: InterPro
  • mitochondrial inner membrane Source: MGI
  • mitochondrial nucleoid Source: UniProtKB
  • mitochondrion Source: MGI
  • protein complex Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion, Mitochondrion nucleoid

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001012711 – 1218DNA polymerase subunit gamma-1Add BLAST1218

Proteomic databases

EPDiP54099.
PaxDbiP54099.
PeptideAtlasiP54099.
PRIDEiP54099.

PTM databases

iPTMnetiP54099.
PhosphoSitePlusiP54099.
SwissPalmiP54099.

Expressioni

Gene expression databases

CleanExiMM_POLG.

Interactioni

Subunit structurei

Heterotrimer composed of a catalytic subunit and a homodimer of accessory subunits.

Binary interactionsi

WithEntry#Exp.IntActNotes
Sod2P096712EBI-863636,EBI-1635071
Tp53P023402EBI-863636,EBI-474016

GO - Molecular functioni

Protein-protein interaction databases

IntActiP54099. 2 interactors.
STRINGi10090.ENSMUSP00000073551.

Structurei

3D structure databases

ProteinModelPortaliP54099.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi517 – 520Poly-Glu4

Sequence similaritiesi

Belongs to the DNA polymerase type-A family.Curated

Phylogenomic databases

eggNOGiKOG3657. Eukaryota.
COG0749. LUCA.
HOGENOMiHOG000176668.
HOVERGENiHBG051400.
InParanoidiP54099.

Family and domain databases

Gene3Di3.30.420.10. 1 hit.
InterProiIPR019760. DNA-dir_DNA_pol_A_CS.
IPR002297. DNA-dir_DNA_pol_A_mt.
IPR001098. DNA-dir_DNA_pol_A_palm_dom.
IPR012337. RNaseH-like_dom.
[Graphical view]
PANTHERiPTHR10267. PTHR10267. 2 hits.
PfamiPF00476. DNA_pol_A. 1 hit.
[Graphical view]
PIRSFiPIRSF000797. DNA_pol_mt. 1 hit.
PRINTSiPR00867. DNAPOLG.
SMARTiSM00482. POLAc. 1 hit.
[Graphical view]
SUPFAMiSSF53098. SSF53098. 2 hits.
PROSITEiPS00447. DNA_POLYMERASE_A. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P54099-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSRLLWKKVA GAKVASGPVP ATARWVASSV LDPVPSDGRP PSQMPSSENG
60 70 80 90 100
QLRLNPLLIQ MLSRGLHEQI FGCGGEMPDE AAVQRSVEHL QKHGLWGQPA
110 120 130 140 150
TPLPDVELRL PRLFGGNLDQ HFRLLAQKQS LPYLEAAASL LEAQLPPEPK
160 170 180 190 200
SWAWAEGWTR YGPEGEAEPV AIPEERALVF DVEVCLAEGT CPTLAVAISP
210 220 230 240 250
SAWYSWCSRR LVEERYSWTS QLSPADLIPL GGSTSASSST KQDGQEQLVV
260 270 280 290 300
GHNVSFDRAH IREQYLIQDS RMRFLDTMSM HMAISGLSSF QRSLWMGAKQ
310 320 330 340 350
GKHKNPAAHK ARAEVPEESQ WSSESSSWDW MDISSANNLA DVHNLYVGGP
360 370 380 390 400
PLEKEPRELF VKGSMRDIRE NFQDLMQYCA RDVWATFEVF QQQLPLFLER
410 420 430 440 450
CPHPVTLAGM LEMGVSYLPV NQNWERYLTE AQNTYEELQR EMKKSLMDLA
460 470 480 490 500
NDACQLLSGE RYKEDPWLWD LEWDLQEFKQ KKAKKVKKPA SASKLPIEGA
510 520 530 540 550
GPFGDPMDQE DPGPPSEEEE LQRSVTAHNR LQQLRSTTDL LPKRPQHLPG
560 570 580 590 600
HPGWYRQLCP RLDDPAWAPG PSLLSLQMRV TPKLMALTWD GFPLHYSDSH
610 620 630 640 650
GWGYLVPGRR DNLTEPPVSP TVESAAVTCP YRAIESLYRK HCLEQGKQQL
660 670 680 690 700
EPQEVDLAEE FLLTDNSAMW QTVEELGCLD VEAEAKMENS GLSQPLVLPA
710 720 730 740 750
ACAPKSSQPT YHHGNGPYND VNIPGCWFFK LPHKDGNNYN VGSPFAKDFL
760 770 780 790 800
PKMEDGTLQA GPGGASGPRA LEINKMISFW RNAHKRISSQ MVVWLPRSAL
810 820 830 840 850
PRVVTRHPAF DEEGHYGAIL PQVVTAGTIT RRAVEPTWLT ASNARPDRVG
860 870 880 890 900
SELKAMVQAP PGYVLVGADV DSQELWIAAV LGDAHFAGMH GCTAFGWMTL
910 920 930 940 950
QGRKSRGTDL HSKTAATVGI SREHAKIFNY GRIYGAGQSF AERLLMQFNH
960 970 980 990 1000
RLTRQEAAEK AQQMYAVTKG LRRYRLSADG EWLVKQLNLP VDRTEDGWVS
1010 1020 1030 1040 1050
LQDLRMIRRE ASRKSRWKKW EVAAERAWTG GTESEMFNKL ESIAMSDTPR
1060 1070 1080 1090 1100
TPVLGCCISR ALEPSVVQGE FITSRVNWVV QSSAVDYLHL MLVAMKWLFE
1110 1120 1130 1140 1150
EFAIDGRFCI SIHDEVRYLV REEDRYRAAL ALQITNLLTR CMFAYKLGLN
1160 1170 1180 1190 1200
DLPQSVAFFS AVDIDQCLRK EVTMDCKTPS NPTGMERRYG IPQGEALDIY
1210
QIIELTKGSL EKRSQPGP
Length:1,218
Mass (Da):136,758
Last modified:March 4, 2015 - v3
Checksum:i55BC904A7318DA26
GO

Sequence cautioni

The sequence AAA98977 differs from that shown. Reason: Frameshift at positions 441, 447, 454 and 1214.Curated
The sequence AAF82772 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti23 – 24AR → EG in AAA98977 (Ref. 1) Curated2
Sequence conflicti27A → S in AAA98977 (Ref. 1) Curated1
Sequence conflicti32D → A in AAA98977 (Ref. 1) Curated1
Sequence conflicti159T → N in AAA98977 (Ref. 1) Curated1
Sequence conflicti228I → T in AAA98977 (Ref. 1) Curated1
Sequence conflicti303 – 308HKNPAA → QQDPAV in AAA98977 (Ref. 1) Curated6
Sequence conflicti324E → D in AAA98977 (Ref. 1) Curated1
Sequence conflicti327Missing in AAA98977 (Ref. 1) Curated1
Sequence conflicti557Q → K in AAA98977 (Ref. 1) Curated1
Sequence conflicti666N → S in AAA98977 (Ref. 1) Curated1
Sequence conflicti861P → T in AAA98977 (Ref. 1) Curated1
Sequence conflicti886F → L in AAA98977 (Ref. 1) Curated1
Sequence conflicti919 – 921GIS → RIH in AAA98977 (Ref. 1) Curated3
Sequence conflicti1024A → S in AAA98977 (Ref. 1) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U53584 mRNA. Translation: AAA98977.1. Frameshift.
AF268975
, AF268970, AF268971, AF268972, AF268973, AF268974 Genomic DNA. Translation: AAF82772.1. Sequence problems.
UniGeneiMm.3616.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U53584 mRNA. Translation: AAA98977.1. Frameshift.
AF268975
, AF268970, AF268971, AF268972, AF268973, AF268974 Genomic DNA. Translation: AAF82772.1. Sequence problems.
UniGeneiMm.3616.

3D structure databases

ProteinModelPortaliP54099.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP54099. 2 interactors.
STRINGi10090.ENSMUSP00000073551.

PTM databases

iPTMnetiP54099.
PhosphoSitePlusiP54099.
SwissPalmiP54099.

Proteomic databases

EPDiP54099.
PaxDbiP54099.
PeptideAtlasiP54099.
PRIDEiP54099.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Organism-specific databases

MGIiMGI:1196389. Polg.

Phylogenomic databases

eggNOGiKOG3657. Eukaryota.
COG0749. LUCA.
HOGENOMiHOG000176668.
HOVERGENiHBG051400.
InParanoidiP54099.

Miscellaneous databases

PROiP54099.
SOURCEiSearch...

Gene expression databases

CleanExiMM_POLG.

Family and domain databases

Gene3Di3.30.420.10. 1 hit.
InterProiIPR019760. DNA-dir_DNA_pol_A_CS.
IPR002297. DNA-dir_DNA_pol_A_mt.
IPR001098. DNA-dir_DNA_pol_A_palm_dom.
IPR012337. RNaseH-like_dom.
[Graphical view]
PANTHERiPTHR10267. PTHR10267. 2 hits.
PfamiPF00476. DNA_pol_A. 1 hit.
[Graphical view]
PIRSFiPIRSF000797. DNA_pol_mt. 1 hit.
PRINTSiPR00867. DNAPOLG.
SMARTiSM00482. POLAc. 1 hit.
[Graphical view]
SUPFAMiSSF53098. SSF53098. 2 hits.
PROSITEiPS00447. DNA_POLYMERASE_A. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDPOG1_MOUSE
AccessioniPrimary (citable) accession number: P54099
Secondary accession number(s): Q9JI28
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: March 4, 2015
Last modified: November 2, 2016
This is version 123 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.