P54081 (TYSY2_BACAM) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Thymidylate synthase 2
Short name=TS 2
Short name=TSase 2
EC=2.1.1.45

Alternative name(s):
Thymidylate synthase B
Short name=TS B
Short name=TSase B

Gene names

Name:	thyA2
Synonyms:	thyB, thyBA

Organism

Bacillus amyloliquefaciens (Bacillus velezensis)

Taxonomic identifier

1390 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	264 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Provides the sole de novo source of dTMP for DNA biosynthesis By similarity. HAMAP MF_00008
Catalytic activity	5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP. HAMAP MF_00008
Pathway	Pyrimidine metabolism; dTTP biosynthesis. HAMAP MF_00008
Subunit structure	Homodimer By similarity. HAMAP MF_00008
Subcellular location	Cytoplasm By similarity HAMAP MF_00008.
Sequence similarities	Belongs to the thymidylate synthase family. Bacterial-type ThyA subfamily.
Sequence caution	The sequence CAA90532.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Nucleotide biosynthesis
Cellular component	Cytoplasm
Molecular function	Methyltransferase Transferase
Gene Ontology (GO)
Biological process	dTMP biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	thymidylate synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 264	264	Thymidylate synthase 2 HAMAP MF_00008		PRO_0000140918
Sites
	Active site	146	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	P54081 [UniParc]. Last modified May 30, 2000. Version 2. Checksum: 3092687D5653105B	FASTA	264	30,350
	10 20 30 40 50 60 MKQYKDLCRH VLENGEKKGD RTGTGTISTF GYQMRFNLQG GFPMLTTKKL HFKSIAHELL 70 80 90 100 110 120 WFLKGDTNVR YLQENGVRIW NEWADENGEL GPVYGSQWRS WRGADGETID QISRLIHDIK 130 140 150 160 170 180 TNPNSRRLIV SAWNVGEIDR MALPPCHCLF QFYVADGKLS CQLYQRSADV FLGVPFNIAS 190 200 210 220 230 240 YALLTMMIAH VTGLEPGEFV HTFGDVHIYQ NHVEQVNLQL TRDVRPLPKL RFARNVDSIF 250 260 DFAFEDFIIE DYDPHPHIKG AVSV

References

[1]	"Cloning of the Bacillus thymidylate synthase genes thyBA and thyBL." Steinborn G., Richter K. Submitted (JUL-1995) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 23844 / P.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z50164 Genomic DNA. Translation: CAA90532.1. Different initiation.
3D structure databases
ProteinModelPortal	P54081.
SMR	P54081. Positions 1-264.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
HAMAP	MF_00008. Thymidy_synth_bact. [Tree]
InterPro	IPR023451. Thymidate_synth/dCMP_Mease. IPR000398. Thymidylate_synthase. IPR020940. Thymidylate_synthase_AS. [Graphical view]
Gene3D	G3DSA:3.30.572.10. Thymidylat_synth_C. 1 hit.
PANTHER	PTHR11549:SF2. Thymidylat_synth_C. 1 hit.
Pfam	PF00303. Thymidylat_synt. 1 hit. [Graphical view]
PRINTS	PR00108. THYMDSNTHASE.
SUPFAM	SSF55831. Thymidylat_synth_C. 1 hit.
TIGRFAMs	TIGR03284. Thym_sym. 2 hits.
PROSITE	PS00091. THYMIDYLATE_SYNTHASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TYSY2_BACAM

Accession

Primary (citable) accession number: P54081

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	May 30, 2000
Last modified:	November 16, 2011
This is version 64 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program