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P54081

- TYSY2_BACAM

UniProt

P54081 - TYSY2_BACAM

Protein

Thymidylate synthase 2

Gene

thyA2

Organism
Bacillus amyloliquefaciens (Bacillus velezensis)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 75 (01 Oct 2014)
      Sequence version 2 (30 May 2000)
      Previous versions | rss
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    • Comment

    Functioni

    Provides the sole de novo source of dTMP for DNA biosynthesis.UniRule annotation

    Catalytic activityi

    5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei146 – 1461UniRule annotation

    GO - Molecular functioni

    1. thymidylate synthase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. dTMP biosynthetic process Source: UniProtKB-HAMAP
    2. dTTP biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Methyltransferase, Transferase

    Keywords - Biological processi

    Nucleotide biosynthesis

    Enzyme and pathway databases

    UniPathwayiUPA00575.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Thymidylate synthase 2UniRule annotation (EC:2.1.1.45UniRule annotation)
    Short name:
    TS 2UniRule annotation
    Short name:
    TSase 2UniRule annotation
    Alternative name(s):
    Thymidylate synthase BUniRule annotation
    Short name:
    TS BUniRule annotation
    Short name:
    TSase BUniRule annotation
    Gene namesi
    Name:thyA2UniRule annotation
    Synonyms:thyB, thyBA
    OrganismiBacillus amyloliquefaciens (Bacillus velezensis)
    Taxonomic identifieri1390 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 264264Thymidylate synthase 2PRO_0000140918Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer.UniRule annotation

    Structurei

    3D structure databases

    ProteinModelPortaliP54081.
    SMRiP54081. Positions 1-264.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the thymidylate synthase family. Bacterial-type ThyA subfamily.UniRule annotation

    Family and domain databases

    Gene3Di3.30.572.10. 1 hit.
    HAMAPiMF_00008. Thymidy_synth_bact.
    InterProiIPR023451. Thymidate_synth/dCMP_Mease.
    IPR000398. Thymidylate_synthase.
    IPR020940. Thymidylate_synthase_AS.
    [Graphical view]
    PfamiPF00303. Thymidylat_synt. 1 hit.
    [Graphical view]
    PRINTSiPR00108. THYMDSNTHASE.
    SUPFAMiSSF55831. SSF55831. 1 hit.
    TIGRFAMsiTIGR03284. thym_sym. 2 hits.
    PROSITEiPS00091. THYMIDYLATE_SYNTHASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P54081-1 [UniParc]FASTAAdd to Basket

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    MKQYKDLCRH VLENGEKKGD RTGTGTISTF GYQMRFNLQG GFPMLTTKKL    50
    HFKSIAHELL WFLKGDTNVR YLQENGVRIW NEWADENGEL GPVYGSQWRS 100
    WRGADGETID QISRLIHDIK TNPNSRRLIV SAWNVGEIDR MALPPCHCLF 150
    QFYVADGKLS CQLYQRSADV FLGVPFNIAS YALLTMMIAH VTGLEPGEFV 200
    HTFGDVHIYQ NHVEQVNLQL TRDVRPLPKL RFARNVDSIF DFAFEDFIIE 250
    DYDPHPHIKG AVSV 264
    Length:264
    Mass (Da):30,350
    Last modified:May 30, 2000 - v2
    Checksum:i3092687D5653105B
    GO

    Sequence cautioni

    The sequence CAA90532.1 differs from that shown. Reason: Erroneous initiation.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z50164 Genomic DNA. Translation: CAA90532.1. Different initiation.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z50164 Genomic DNA. Translation: CAA90532.1 . Different initiation.

    3D structure databases

    ProteinModelPortali P54081.
    SMRi P54081. Positions 1-264.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Enzyme and pathway databases

    UniPathwayi UPA00575 .

    Family and domain databases

    Gene3Di 3.30.572.10. 1 hit.
    HAMAPi MF_00008. Thymidy_synth_bact.
    InterProi IPR023451. Thymidate_synth/dCMP_Mease.
    IPR000398. Thymidylate_synthase.
    IPR020940. Thymidylate_synthase_AS.
    [Graphical view ]
    Pfami PF00303. Thymidylat_synt. 1 hit.
    [Graphical view ]
    PRINTSi PR00108. THYMDSNTHASE.
    SUPFAMi SSF55831. SSF55831. 1 hit.
    TIGRFAMsi TIGR03284. thym_sym. 2 hits.
    PROSITEi PS00091. THYMIDYLATE_SYNTHASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning of the Bacillus thymidylate synthase genes thyBA and thyBL."
      Steinborn G., Richter K.
      Submitted (JUL-1995) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 23844 / P.

    Entry informationi

    Entry nameiTYSY2_BACAM
    AccessioniPrimary (citable) accession number: P54081
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: May 30, 2000
    Last modified: October 1, 2014
    This is version 75 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3