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P54081 (TYSY2_BACAM) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 74. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Thymidylate synthase 2

Short name=TS 2
Short name=TSase 2
EC=2.1.1.45
Alternative name(s):
Thymidylate synthase B
Short name=TS B
Short name=TSase B
Gene names
Name:thyA2
Synonyms:thyB, thyBA
OrganismBacillus amyloliquefaciens (Bacillus velezensis)
Taxonomic identifier1390 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

Protein attributes

Sequence length264 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Provides the sole de novo source of dTMP for DNA biosynthesis By similarity. HAMAP-Rule MF_00008

Catalytic activity

5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP. HAMAP-Rule MF_00008

Pathway

Pyrimidine metabolism; dTTP biosynthesis. HAMAP-Rule MF_00008

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_00008

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00008.

Sequence similarities

Belongs to the thymidylate synthase family. Bacterial-type ThyA subfamily.

Sequence caution

The sequence CAA90532.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
   Biological processNucleotide biosynthesis
   Cellular componentCytoplasm
   Molecular functionMethyltransferase
Transferase
Gene Ontology (GO)
   Biological_processdTMP biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-HAMAP

dTTP biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionthymidylate synthase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 264264Thymidylate synthase 2 HAMAP-Rule MF_00008
PRO_0000140918

Sites

Active site1461 By similarity

Sequences

Sequence LengthMass (Da)Tools
P54081 [UniParc].

Last modified May 30, 2000. Version 2.
Checksum: 3092687D5653105B

FASTA26430,350
        10         20         30         40         50         60 
MKQYKDLCRH VLENGEKKGD RTGTGTISTF GYQMRFNLQG GFPMLTTKKL HFKSIAHELL 

        70         80         90        100        110        120 
WFLKGDTNVR YLQENGVRIW NEWADENGEL GPVYGSQWRS WRGADGETID QISRLIHDIK 

       130        140        150        160        170        180 
TNPNSRRLIV SAWNVGEIDR MALPPCHCLF QFYVADGKLS CQLYQRSADV FLGVPFNIAS 

       190        200        210        220        230        240 
YALLTMMIAH VTGLEPGEFV HTFGDVHIYQ NHVEQVNLQL TRDVRPLPKL RFARNVDSIF 

       250        260 
DFAFEDFIIE DYDPHPHIKG AVSV 

« Hide

References

[1]"Cloning of the Bacillus thymidylate synthase genes thyBA and thyBL."
Steinborn G., Richter K.
Submitted (JUL-1995) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 23844 / P.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z50164 Genomic DNA. Translation: CAA90532.1. Different initiation.

3D structure databases

ProteinModelPortalP54081.
SMRP54081. Positions 1-264.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayUPA00575.

Family and domain databases

Gene3D3.30.572.10. 1 hit.
HAMAPMF_00008. Thymidy_synth_bact.
InterProIPR023451. Thymidate_synth/dCMP_Mease.
IPR000398. Thymidylate_synthase.
IPR020940. Thymidylate_synthase_AS.
[Graphical view]
PfamPF00303. Thymidylat_synt. 1 hit.
[Graphical view]
PRINTSPR00108. THYMDSNTHASE.
SUPFAMSSF55831. SSF55831. 1 hit.
TIGRFAMsTIGR03284. thym_sym. 2 hits.
PROSITEPS00091. THYMIDYLATE_SYNTHASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTYSY2_BACAM
AccessionPrimary (citable) accession number: P54081
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: May 30, 2000
Last modified: February 19, 2014
This is version 74 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways