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Protein

Protein ASI1

Gene

ASI1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Negative regulator of SPS-sensor signaling. Together with ASI2 and ASI3, prevents the unprocessed precursor forms of STP1 and STP2 that escape cytoplasmic anchoring from inducing SPS-sensor-regulated genes in the absence of inducing signals.2 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri568 – 608RING-type; atypicalAdd BLAST41

GO - Molecular functioni

  • metal ion binding Source: UniProtKB-KW
  • ubiquitin-protein transferase activity Source: SGD

GO - Biological processi

  • cellular response to amino acid stimulus Source: SGD
  • transcription factor catabolic process Source: SGD
  • ubiquitin-dependent protein catabolic process Source: SGD

Keywordsi

LigandMetal-binding, Zinc

Enzyme and pathway databases

BioCyciYEAST:G3O-32814-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein ASI1
Alternative name(s):
Amino acid sensor-independent protein 1
Gene namesi
Name:ASI1
Ordered Locus Names:YMR119W
ORF Names:YM8564.01, YM9718.18
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIII

Organism-specific databases

EuPathDBiFungiDB:YMR119W.
SGDiS000004725. ASI1.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 69Perinuclear spaceSequence analysisAdd BLAST69
Transmembranei70 – 90HelicalSequence analysisAdd BLAST21
Topological domaini91 – 116NuclearSequence analysisAdd BLAST26
Transmembranei117 – 137HelicalSequence analysisAdd BLAST21
Topological domaini138 – 152Perinuclear spaceSequence analysisAdd BLAST15
Transmembranei153 – 173HelicalSequence analysisAdd BLAST21
Topological domaini174 – 209NuclearSequence analysisAdd BLAST36
Transmembranei210 – 230HelicalSequence analysisAdd BLAST21
Topological domaini231 – 273Perinuclear spaceSequence analysisAdd BLAST43
Transmembranei274 – 294HelicalSequence analysisAdd BLAST21
Topological domaini295 – 624NuclearSequence analysisAdd BLAST330

GO - Cellular componenti

  • Asi complex Source: SGD
  • integral component of membrane Source: UniProtKB-KW
  • nuclear inner membrane Source: SGD

Keywords - Cellular componenti

Membrane, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi583C → S in ASI1-21HA; when associated with S-585; abolishes function. 1 Publication1
Mutagenesisi585C → S in ASI1-21HA; when associated with S-583; abolishes function. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002032951 – 624Protein ASI1Add BLAST624

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi2N-linked (GlcNAc...) asparagine2 Publications1
Glycosylationi19N-linked (GlcNAc...) asparagine2 Publications1
Glycosylationi29N-linked (GlcNAc...) asparagine2 Publications1

Post-translational modificationi

Glycosylation is not required for ASI1 function.2 Publications

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiP54074.
PRIDEiP54074.

Interactioni

Subunit structurei

Interacts with ASI3.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
ASI3P539835EBI-27241,EBI-28603

Protein-protein interaction databases

BioGridi35296. 90 interactors.
DIPiDIP-7733N.
IntActiP54074. 3 interactors.
MINTiMINT-512982.
STRINGi4932.YMR119W.

Structurei

3D structure databases

ProteinModelPortaliP54074.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri568 – 608RING-type; atypicalAdd BLAST41

Keywords - Domaini

Transmembrane, Transmembrane helix, Zinc-finger

Phylogenomic databases

GeneTreeiENSGT00530000069536.
HOGENOMiHOG000034100.
InParanoidiP54074.
OMAiLWPCRCF.
OrthoDBiEOG092C3BJA.

Sequencei

Sequence statusi: Complete.

P54074-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNSSTSSENV FINSFSYLNQ TSQAVISGNS TFANVINFPY RLGLSFIGAV
60 70 80 90 100
NLQYEQTVKS EEIPPTLRSV FDTIGFFFSP YAIFCFVIAI VLNRFVVFYA
110 120 130 140 150
VLNNGSRRTL PLWLSNVFHV SAVVVLAMVS LGPLTLGKDF KILGDPAFAQ
160 170 180 190 200
EKFLLNIFYA FAYSYCVETI FTIMRNSSPL EGTDYSLFEL SIQFYTMTNN
210 220 230 240 250
NTKFLDSPDY IIDCSMAILS RILIHLVEIF RLRNYRLLFS TIMNLCHICY
260 270 280 290 300
LGIRVKQGGW KSLPFSVKFR HFPKLFSVSI ICLSLLIFKL SCLIRWDPFG
310 320 330 340 350
KSRNSCELLQ FYPLSRNWKK YLNYTGEEDF SAMATKFALL LCSGTELMEK
360 370 380 390 400
GIRREFPAIN IPDNVNEKFF ISGYLNELSK PYKENTSISF PKKNSSILKQ
410 420 430 440 450
RFFLMFPKSI IWIMKKLVGQ VFFGFRDNKD EDIPDNDPSK MLKITKTNSL
460 470 480 490 500
NNSAGHKEDI ELELLNTSDD EYSEDYEPSE VESLGDSDEE NLEEDSLIFN
510 520 530 540 550
ETRDALLDLF SSEDNEVHTD YNWIMSTSRI LQQKLLSDKT LTRASILDTK
560 570 580 590 600
LSEVDETFGT ESDFDLSCAV CKVNERNTVL WPCRCFAICE DCRISLGLRG
610 620
FSTCVCCRSK VHGYCKVHPV SDSK
Length:624
Mass (Da):71,444
Last modified:October 1, 1996 - v1
Checksum:iE2F5149F89A09655
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z49702 Genomic DNA. Translation: CAA89757.1.
Z49273 Genomic DNA. Translation: CAA89268.1.
BK006946 Genomic DNA. Translation: DAA10016.1.
PIRiS54581.
RefSeqiNP_013837.1. NM_001182619.1.

Genome annotation databases

EnsemblFungiiYMR119W; YMR119W; YMR119W.
GeneIDi855147.
KEGGisce:YMR119W.

Similar proteinsi

Entry informationi

Entry nameiASI1_YEAST
AccessioniPrimary (citable) accession number: P54074
Secondary accession number(s): D6VZU2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: July 5, 2017
This is version 117 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  2. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names