P54070 (KTR6_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Mannosyltransferase KTR6
EC=2.4.1.-

Alternative name(s):
Mannosylphosphate transferase MNN6

Gene names

Name:	KTR6
Synonyms:	MNN6
Ordered Locus Names:	YPL053C
ORF Names:	LPE19C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	446 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Glycosyltransferase. Required for addition of mannosylphosphate in yeast mannan. Recognizes any oligosaccharides with at least one alpha-1,2-linked mannobiose unit.
Catalytic activity	Transfers an alpha-D-mannosyl residue from GDP-mannose into lipid-linked oligosaccharide, forming an alpha-(1->2)-D-mannosyl-D-mannose linkage.
Pathway	Protein modification; protein glycosylation.
Subcellular location	Membrane; Single-pass type II membrane protein Probable.
Miscellaneous	Present with 4380 molecules/cell in log phase SD medium. Ref.5
Sequence similarities	Belongs to the glycosyltransferase 15 family.

Ontologies

Keywords
Cellular component	Membrane
Domain	Signal-anchor Transmembrane Transmembrane helix
Molecular function	Glycosyltransferase Transferase
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	fungal-type cell wall organization Inferred from mutant phenotype Ref.4. Source: SGD protein N-linked glycosylation Inferred from mutant phenotype Ref.1. Source: SGD
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	mannosylphosphate transferase activity Inferred from mutant phenotype Ref.1. Source: SGD mannosyltransferase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 446	446	Mannosyltransferase KTR6		PRO_0000208247
Regions
	Topological domain	1 – 8	8	Cytoplasmic Potential
	Transmembrane	9 – 29	21	Helical; Signal-anchor for type II membrane protein; Potential
	Topological domain	30 – 446	417	Lumenal Potential
	Region	30 – 114	85	Stem region By similarity
	Region	115 – 446	332	Catalytic By similarity
Sites
	Active site	334	1	Nucleophile Potential
Amino acid modifications
	Glycosylation	82	1	N-linked (GlcNAc...) Potential
	Glycosylation	98	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	P54070 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 72F06428B8722803	FASTA	446	52,128
	10 20 30 40 50 60 MHVLLSKKIA RFLLISFVFV LALMVTINHP KTKQMSEQYV TPYLPKSLQP IAKISAEEQR 70 80 90 100 110 120 RIQSEQEEAE LKQSLEGEAI RNATVNAIKE KIKSYGGNET TLGFMVPSYI NHRGSPPKAC 130 140 150 160 170 180 FVSLITERDS MTQILQSIDE VQVKFNKNFA YPWVFISQGE LDGMKQEMIR QAITDSMNGD 190 200 210 220 230 240 PELINIKFAE IPADEWVYPE WIDENKAAES LISLANVPDG DSRAVRYQAR YFAGFFWRHP 250 260 270 280 290 300 VLDEFDWYWR VDPGIKLYCD IDHDLFRWMQ DEGKVFGFTL SMSEAKEANE KIWDVTKKFA 310 320 330 340 350 360 KDFPKFISEN NFKSFITKKD SEDFNNCEFT SNFEIGNLNF YRSPAYRKFF NYIDEEGGIF 370 380 390 400 410 420 YWKWSDSIIH TIGLSMLLPK DKIHFFENIG FHYDKYNNCP LNDDIWNQYN CNCDQGNDFT 430 440 FRSGSCGGHY FDIMKKDKPE GWDRLP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"MNN6, a member of the KRE2/MNT1 family, is the gene for mannosylphosphate transfer in Saccharomyces cerevisiae." Wang X.-H., Nakayama K., Shimma Y., Tanaka A., Jigami Y. J. Biol. Chem. 272:18117-18124(1997) [PubMed: 9218445] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CHARACTERIZATION. Strain: S288c / GRF88.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI." Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M. , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J. Nature 387:103-105(1997) [PubMed: 9169875] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Completion of the Saccharomyces cerevisiae genome sequence allows identification of KTR5, KTR6 and KTR7 and definition of the nine-membered KRE2/MNT1 mannosyltransferase gene family in this organism." Lussier M., Sdicu A.-M., Winnett E., Vo D.H., Sheraton J., Duesterhoeft A., Storms R.K., Bussey H. Yeast 13:267-274(1997) [PubMed: 9090056] [Abstract] Cited for: CHARACTERIZATION.
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U43922 Genomic DNA. Translation: AAC49761.1. U39205 Genomic DNA. Translation: AAB68312.1. BK006949 Genomic DNA. Translation: DAA11377.1.
PIR	S61089.
RefSeq	NP_015272.1. NM_001183867.1.
3D structure databases
ProteinModelPortal	P54070.
SMR	P54070. Positions 99-445.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-5329N.
IntAct	P54070. 4 interactions.
MINT	MINT-548656.
STRING	P54070.
Protein family/group databases
CAZy	GT15. Glycosyltransferase Family 15.
Proteomic databases
PeptideAtlas	P54070.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YPL053C; YPL053C; YPL053C.
GeneID	856054.
KEGG	sce:YPL053C.
NMPDR	fig|4932.3.peg.6405.
Organism-specific databases
CYGD	YPL053c.
SGD	S000005974. KTR6.
Phylogenomic databases
eggNOG	fuNOG04371.
GeneTree	EFGT00050000003570.
HOGENOM	HBG396033.
OMA	ANIRANW.
Gene expression databases
ArrayExpress	P54070.
Genevestigator	P54070.
GermOnline	YPL053C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR002685. Glyco_trans_15. [Graphical view]
KO	K05537.
Pfam	PF01793. Glyco_transf_15. 1 hit. [Graphical view]
PIRSF	PIRSF018153. Glyco_trans_15. 1 hit.
ProtoNet	Search...
Other
NextBio	981017.

Entry information

Entry name

KTR6_YEAST

Accession

Primary (citable) accession number: P54070
Secondary accession number(s): D6W3W1

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	December 14, 2011
This is version 103 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program