P54066 (RL7A_METJA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
50S ribosomal protein L7Ae

Gene names

Name:	rpl7ae
Ordered Locus Names:	MJ1203

Organism

Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii) [Reference proteome] [HAMAP]

Taxonomic identifier

243232 [NCBI]

Taxonomic lineage

cellular organisms › Archaea › Euryarchaeota › Methanococci › Methanococcales › Methanocaldococcaceae › Methanocaldococcus › Methanocaldococcus jannaschii

Protein attributes

Sequence length	117 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Multifunctional RNA-binding protein that recognizes the K-turn motif in ribosomal RNA, box H/ACA, box C/D and box C'/D' sRNAs. HAMAP-Rule MF_00326
Sequence similarities	Belongs to the ribosomal protein L7Ae family.

Ontologies

Keywords
Ligand	RNA-binding
Molecular function	Ribonucleoprotein Ribosomal protein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	ribosome biogenesis Inferred from electronic annotation. Source: InterPro translation Inferred from electronic annotation. Source: HAMAP
Cellular_component	ribosome Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	RNA binding Inferred from electronic annotation. Source: UniProtKB-KW structural constituent of ribosome Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
MJ0694	Q58105	4	EBI-2944269,EBI-2944259

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 117

117

50S ribosomal protein L7Ae HAMAP-Rule MF_00326

PRO_0000136793

Secondary structure

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117

Helix Strand Turn

Details...

Helix

11 – 22

12

Beta strand

25 – 30

6

Helix

31 – 39

9

Beta strand

44 – 50

7

Helix

55 – 57

3

Turn

58 – 60

3

Helix

61 – 67

7

Beta strand

72 – 76

5

Helix

78 – 84

7

Beta strand

92 – 98

7

Helix

102 – 115

14

Sequences

	Sequence		Length	Mass (Da)
	P54066 [UniParc]. Last modified July 15, 1998. Version 2. Checksum: 7CAB706F09371BA6	FASTA	117	12,686
	10 20 30 40 50 60 MAVYVKFKVP EEIQKELLDA VAKAQKIKKG ANEVTKAVER GIAKLVIIAE DVKPEEVVAH 70 80 90 100 110 LPYLCEEKGI PYAYVASKQD LGKAAGLEVA ASSVAIINEG DAEELKVLIE KVNVLKQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii." Bult C.J., White O., Olsen G.J., Zhou L., Fleischmann R.D., Sutton G.G., Blake J.A., FitzGerald L.M., Clayton R.A., Gocayne J.D., Kerlavage A.R., Dougherty B.A., Tomb J.-F., Adams M.D., Reich C.I., Overbeek R., Kirkness E.F., Weinstock K.G. , Merrick J.M., Glodek A., Scott J.L., Geoghagen N.S.M., Weidman J.F., Fuhrmann J.L., Nguyen D., Utterback T.R., Kelley J.M., Peterson J.D., Sadow P.W., Hanna M.C., Cotton M.D., Roberts K.M., Hurst M.A., Kaine B.P., Borodovsky M., Klenk H.-P., Fraser C.M., Smith H.O., Woese C.R., Venter J.C. Science 273:1058-1073(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440.
[2]	"Structure of protein L7Ae bound to a K-turn derived from an archaeal box H/ACA sRNA at 1.8 A resolution." Hamma T., Ferre-D'Amare A.R. Structure 12:893-903(2004) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS).
[3]	"The crystal structure of the Methanocaldococcus jannaschii multifunctional L7Ae RNA-binding protein reveals an induced-fit interaction with the box C/D RNAs." Suryadi J., Tran E.J., Maxwell E.S., Brown B.A. II Biochemistry 44:9657-9672(2005) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.45 ANGSTROMS).

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

L77117 Genomic DNA. Translation: AAB99207.1.

RefSeq

NP_248198.1. NC_000909.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1RA4	X-ray	1.86	A	1-117	[»]
1SDS	X-ray	1.80	A/B/C	1-117	[»]
1XBI	X-ray	1.45	A	1-117	[»]
3PAF	X-ray	1.70	A/B	1-117	[»]

ProteinModelPortal

P54066.

SMR

P54066. Positions 1-117.

ModBase

Search...

Protein-protein interaction databases

IntAct

P54066. 2 interactions.

STRING

243232.MJ1203.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

AAB99207; AAB99207; MJ_1203.

GeneID

1452098.

KEGG

mja:MJ_1203.

Phylogenomic databases

eggNOG

COG1358.

KO

K02936.

OMA

EDVDPPE.

ProtClustDB

PRK04175.

Family and domain databases

HAMAP

MF_00326. Ribosomal_L7Ae_Arch.

InterPro

IPR004038. Ribosomal_L7Ae/L30e/S12e/Gad45.
IPR018492. Ribosomal_L7Ae/L8/Nhp2.
IPR022481. Ribosomal_L7Ae_arc.
IPR004037. Ribosomal_L7Ae_CS.
IPR000948. Ribosomal_L7Ae_prok.
[Graphical view]

Pfam

PF01248. Ribosomal_L7Ae. 1 hit.
[Graphical view]

PRINTS

PR00881. L7ARS6FAMILY.
PR00884. RIBOSOMALHS6.

TIGRFAMs

TIGR03677. rpl7ae. 1 hit.

PROSITE

PS01082. RIBOSOMAL_L7AE. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P54066.

Entry information

Entry name

RL7A_METJA

Accession

Primary (citable) accession number: P54066

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	July 15, 1998
Last modified:	May 1, 2013
This is version 85 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program