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P54001 (P4HA1_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified November 16, 2011. Version 86. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Prolyl 4-hydroxylase subunit alpha-1
Short name=4-PH alpha-1
EC=1.14.11.2
Alternative name(s):
Procollagen-proline,2-oxoglutarate-4-dioxygenase subunit alpha-1
Gene names
Name:P4ha1
Synonyms:P4ha
OrganismRattus norvegicus (Rat)
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length534 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Catalyzes the post-translational formation of 4-hydroxyproline in -Xaa-Pro-Gly- sequences in collagens and other proteins By similarity.

Catalytic activity

L-proline-[procollagen] + 2-oxoglutarate + O2 = trans-4-hydroxy-L-proline-[procollagen] + succinate + CO2.

Cofactor

Binds 1 Fe2+ ion per subunit By similarity.

Ascorbate By similarity.

Subunit structure

Heterotetramer of two alpha-1 chains and two beta chains (the beta chain is the multi-functional PDI) By similarity.

Subcellular location

Endoplasmic reticulum lumen By similarity.

Sequence similarities

Belongs to the P4HA family.

Contains 1 Fe2OG dioxygenase domain.

Contains 1 TPR repeat.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1717 By similarity
Chain18 – 534517Prolyl 4-hydroxylase subunit alpha-1
PRO_0000022725

Regions

Repeat205 – 23834TPR
Domain411 – 519109Fe2OG dioxygenase

Sites

Metal binding4291Iron By similarity
Metal binding4311Iron By similarity
Metal binding5001Iron By similarity
Binding site51012-oxoglutarate Potential

Amino acid modifications

Glycosylation1131N-linked (GlcNAc...) Potential
Glycosylation2591N-linked (GlcNAc...) Potential

Experimental info

Sequence conflict265 – 2673DQS → ERA in CAA55546. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P54001 [UniParc].

Last modified September 13, 2005. Version 2.
Checksum: C44659978281FF30

FASTA53460,898
        10         20         30         40         50         60 
MIWGVLMMGI LLPQCSAHPG FFTSIGQMTD LIHNEKDLVT SLKDYIKAEE DKLEQIKKWA 

        70         80         90        100        110        120 
EKLDRLTSTA TKDPEGFVGH PVNAFKLMKR LNTEWSELEN LILKDMSDGF ISNLTIQRQY 

       130        140        150        160        170        180 
FPNDEDQVGA AKALFRLQDT YNLDTNTISK GNLPGVKHKS FLTAEDCFEL GKVAYTEADY 

       190        200        210        220        230        240 
YHTELWMEQA LMQLEEGEMS TVDKVSVLDY LSYAVYQQGD LDKALLLTKK LLELDPEHQR 

       250        260        270        280        290        300 
ANGNLVYFEY IMSKEKDANK SASGDQSDQK TTPKKKGIAV DYLPERQKYE MLCRGEGIKM 

       310        320        330        340        350        360 
TPRRQKRLFC RYHDGNRNPK FILAPAKQED EWDKPRIIRF HDIISDAEIE IVKDLAKPRL 

       370        380        390        400        410        420 
SRATVHDPET GKLTTAQYRV SKSAWLSGYE DPVVSRINMR IQDLTGLDVS TAEELQVANY 

       430        440        450        460        470        480 
GVGGQYEPHF DFARKDEPDA FRELGTGNRI ATWLFYMSDV SAGGATVFPE VGASVWPKKG 

       490        500        510        520        530 
TAVFWYNLFA SGEGDYSTRH AACPVLVGNK WVSNKWLHER GQEFRRPCTL SELE

« Hide

References

« Hide 'large scale' references
[1]"The complete cDNA derived sequence of the rat prolyl 4-hydroxylase alpha subunit."
Hopkinson I., Smith S.A., Donne A., Gregory H., Franklin T.J., Grant M.E., Rosamond J.
Gene 149:391-392(1994) [PubMed: 7959029] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Testis.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		X78949 mRNA. Translation: CAA55546.1.
BC078703 mRNA. Translation: AAH78703.1.
IPIIPI00209863.
PIRS44204.
RefSeqNP_742059.2. NM_172062.2.
UniGeneRn.8531.

3D structure databases

ProteinModelPortalP54001.
SMRP54001. Positions 161-254.
ModBaseSearch...

Protein-protein interaction databases

IntActP54001. 1 interaction.

Proteomic databases

PRIDEP54001.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID64475.
KEGGrno:64475.
UCSCNM_172062. rat.

Organism-specific databases

CTD5033.
RGD621000. P4ha1.

Phylogenomic databases

eggNOGmaNOG06898.
HOVERGENHBG006834.

Enzyme and pathway databases

BRENDA1.14.11.2. 5301.

Gene expression databases

GenevestigatorP54001.

Family and domain databases

InterProIPR005123. Oxoglutarate/Fe-dep_oxygenase.
IPR006620. Pro_4_hyd_alph.
IPR013547. Pro_4_hyd_alph_N.
IPR013026. TPR-contain.
IPR011990. TPR-like_helical.
IPR019734. TPR_repeat.
[Graphical view]
Gene3DG3DSA:1.25.40.10. TPR-like_helical. 1 hit.
KOK00472.
PfamPF03171. 2OG-FeII_Oxy. 1 hit.
PF08336. P4Ha_N. 1 hit.
[Graphical view]
SMARTSM00702. P4Hc. 1 hit.
[Graphical view]
PROSITEPS51471. FE2OG_OXY. 1 hit.
PS50005. TPR. 1 hit.
PS50293. TPR_REGION. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio613270.

Entry information

Entry nameP4HA1_RAT
AccessionPrimary (citable) accession number: P54001
Secondary accession number(s): Q6AZ74
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: September 13, 2005
Last modified: November 16, 2011
This is version 86 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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