Prolyl 4-hydroxylase subunit alpha-1 precursor

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Reviewed, UniProtKB/Swiss-Prot P54001 (P4HA1_RAT)

Last modified November 25, 2008. Version 65. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
    Prolyl 4-hydroxylase subunit alpha-1
    EC=1.14.11.2
Alternative name(s):
    4-PH alpha-1
    Procollagen-proline,2-oxoglutarate-4-dioxygenase subunit alpha-1

Gene names

Name:	P4ha1
Synonyms:	P4ha

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

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Protein attributes

Sequence length	534 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

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General annotation (Comments)

Function	Catalyzes the post-translational formation of 4-hydroxyproline in -Xaa-Pro-Gly- sequences in collagens and other proteins By similarity.
Catalytic activity	Procollagen L-proline + 2-oxoglutarate + O(2) = procollagen trans-4-hydroxy-L-proline + succinate + CO(2).
Cofactor	Binds 1 Fe(2+) ion per subunit By similarity. Ascorbate By similarity.
Subunit structure	Heterotetramer of two alpha-1 chains and two beta chains (the beta chain is the multi-functional PDI) By similarity.
Subcellular location	Endoplasmic reticulum lumenBy similarity.
Sequence similarities	Belongs to the P4HA family. Contains 1 PKHD (prolyl/lysyl hydroxylase) domain. Contains 1 TPR repeat.

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Ontologies

Keywords
Cellular component	Endoplasmic reticulum
Domain	Signal TPR repeat
Ligand	Iron Metal-binding Vitamin C
Molecular function	Dioxygenase Oxidoreductase
PTM	Glycoprotein
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW protein metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	endoplasmic reticulum Inferred from electronic annotation. Source: UniProtKB-KW endoplasmic reticulum lumen Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	L-ascorbic acid binding Inferred from electronic annotation. Source: UniProtKB-KW iron ion binding Inferred from electronic annotation. Source: InterPro oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen Inferred from electronic annotation. Source: UniProtKB-KW procollagen-proline 4-dioxygenase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 17

By similarity

Chain

18 – 534

517

Prolyl 4-hydroxylase subunit alpha-1

PRO_0000022725

Regions

Repeat

205 – 238

TPR

Domain

335 – 518

184

PKHD

Sites

Metal binding

429

Iron By similarity

Metal binding

431

Iron By similarity

Metal binding

500

Iron By similarity

Binding site

510

2-oxoglutarate Potential

Amino acid modifications

Glycosylation

113

N-linked (GlcNAc...) Potential

Glycosylation

259

N-linked (GlcNAc...) Potential

Experimental info

Sequence conflict

265 – 267

DQS → ERA in CAA55546. Ref.1

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Sequences

Sequence

Length

Mass (Da)

Tools

P54001-1 [UniParc].

Last modified September 13, 2005. Version 2.
Checksum: C44659978281FF30
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FASTA

534

60,898

        10         20         30         40         50         60 
MIWGVLMMGI LLPQCSAHPG FFTSIGQMTD LIHNEKDLVT SLKDYIKAEE DKLEQIKKWA 

        70         80         90        100        110        120 
EKLDRLTSTA TKDPEGFVGH PVNAFKLMKR LNTEWSELEN LILKDMSDGF ISNLTIQRQY 

       130        140        150        160        170        180 
FPNDEDQVGA AKALFRLQDT YNLDTNTISK GNLPGVKHKS FLTAEDCFEL GKVAYTEADY 

       190        200        210        220        230        240 
YHTELWMEQA LMQLEEGEMS TVDKVSVLDY LSYAVYQQGD LDKALLLTKK LLELDPEHQR 

       250        260        270        280        290        300 
ANGNLVYFEY IMSKEKDANK SASGDQSDQK TTPKKKGIAV DYLPERQKYE MLCRGEGIKM 

       310        320        330        340        350        360 
TPRRQKRLFC RYHDGNRNPK FILAPAKQED EWDKPRIIRF HDIISDAEIE IVKDLAKPRL 

       370        380        390        400        410        420 
SRATVHDPET GKLTTAQYRV SKSAWLSGYE DPVVSRINMR IQDLTGLDVS TAEELQVANY 

       430        440        450        460        470        480 
GVGGQYEPHF DFARKDEPDA FRELGTGNRI ATWLFYMSDV SAGGATVFPE VGASVWPKKG 

       490        500        510        520        530 
TAVFWYNLFA SGEGDYSTRH AACPVLVGNK WVSNKWLHER GQEFRRPCTL SELE

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The complete cDNA derived sequence of the rat prolyl 4-hydroxylase alpha subunit." Hopkinson I., Smith S.A., Donne A., Gregory H., Franklin T.J., Grant M.E., Rosamond J. Gene 149:391-392(1994) [PubMed: 7959029] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Testis.

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Cross-references

Sequence databases
	X78949 mRNA. Translation: CAA55546.1. BC078703 mRNA. Translation: AAH78703.1.
PIR	S44204.
RefSeq	NP_742059.2.
UniGene	Rn.8531
3D structure databases
SMR	P54001. Positions 161-254.
ModBase	Search...
Genome annotation databases
GeneID	64475.
KEGG	rno:64475.
Organism-specific databases
RGD	621000. P4ha1.
Phylogenomic databases
HOVERGEN	P54001.
Family and domain databases
InterPro	IPR005123. 2OG-FeII_Oase. IPR006620. Pro_4_hyd_alph. IPR013547. Pro_4_hyd_alph_N. IPR011990. TPR-like_helical. IPR013105. TPR_2. IPR013026. TPR_region. [Graphical view]
Gene3D	G3DSA:1.25.40.10. TPR-like_helical. 1 hit.
Pfam	PF03171. 2OG-FeII_Oxy. 1 hit. PF08336. P4Ha_N. 1 hit. PF07719. TPR_2. 1 hit. [Graphical view]
SMART	SM00702. P4Hc. 1 hit. [Graphical view]
PROSITE	PS50005. TPR. 1 hit. PS50293. TPR_REGION. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	613270.

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Entry information

Entry name

P4HA1_RAT

Accession

Primary (citable) accession number: P54001
Secondary accession number(s): Q6AZ74

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	September 13, 2005
Last modified:	November 25, 2008
This is version 65 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

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Relevant documents

SIMILARITY comments

Index of protein domains and families

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Keywords

Gene Ontology (GO)

Sequence annotation (Features)

Molecule processing

Regions

Sites

Amino acid modifications

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Family and domain databases

Other Resources

Entry information

Relevant documents