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Protein

Prolyl 4-hydroxylase subunit alpha-1

Gene

P4ha1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes the post-translational formation of 4-hydroxyproline in -Xaa-Pro-Gly- sequences in collagens and other proteins.By similarity

Catalytic activityi

L-proline-[procollagen] + 2-oxoglutarate + O2 = trans-4-hydroxy-L-proline-[procollagen] + succinate + CO2.

Cofactori

Protein has several cofactor binding sites:
  • Fe2+PROSITE-ProRule annotationNote: Binds 1 Fe2+ ion per subunit.PROSITE-ProRule annotation
  • L-ascorbateBy similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi429 – 4291IronPROSITE-ProRule annotation
Metal bindingi431 – 4311IronPROSITE-ProRule annotation
Metal bindingi500 – 5001IronPROSITE-ProRule annotation
Binding sitei510 – 51012-oxoglutaratePROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

  • collagen fibril organization Source: Ensembl
  • peptidyl-proline hydroxylation to 4-hydroxy-L-proline Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Dioxygenase, Oxidoreductase

Keywords - Ligandi

Iron, Metal-binding, Vitamin C

Enzyme and pathway databases

BRENDAi1.14.11.2. 5301.
ReactomeiR-RNO-1650814. Collagen biosynthesis and modifying enzymes.

Names & Taxonomyi

Protein namesi
Recommended name:
Prolyl 4-hydroxylase subunit alpha-1 (EC:1.14.11.2)
Short name:
4-PH alpha-1
Alternative name(s):
Procollagen-proline,2-oxoglutarate-4-dioxygenase subunit alpha-1
Gene namesi
Name:P4ha1
Synonyms:P4ha
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 20

Organism-specific databases

RGDi621000. P4ha1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum

Pathology & Biotechi

Chemistry

ChEMBLiCHEMBL3301.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1717By similarityAdd
BLAST
Chaini18 – 534517Prolyl 4-hydroxylase subunit alpha-1PRO_0000022725Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi113 – 1131N-linked (GlcNAc...)Sequence analysis
Glycosylationi259 – 2591N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiP54001.
PRIDEiP54001.

Interactioni

Subunit structurei

Heterotetramer of two alpha-1 chains and two beta chains (the beta chain is the multi-functional PDI).By similarity

Protein-protein interaction databases

IntActiP54001. 1 interaction.
STRINGi10116.ENSRNOP00000064405.

Chemistry

BindingDBiP54001.

Structurei

3D structure databases

ProteinModelPortaliP54001.
SMRiP54001. Positions 161-254.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati205 – 23834TPRAdd
BLAST
Domaini411 – 519109Fe2OG dioxygenasePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the P4HA family.Curated
Contains 1 Fe2OG dioxygenase domain.PROSITE-ProRule annotation
Contains 1 TPR repeat.PROSITE-ProRule annotation

Keywords - Domaini

Signal, TPR repeat

Phylogenomic databases

eggNOGiKOG1591. Eukaryota.
ENOG410XS5J. LUCA.
GeneTreeiENSGT00390000018885.
HOVERGENiHBG006834.
InParanoidiP54001.
KOiK00472.
OMAiFLTVEDC.
OrthoDBiEOG7W6WKC.
PhylomeDBiP54001.

Family and domain databases

Gene3Di1.25.40.10. 2 hits.
InterProiIPR005123. Oxoglu/Fe-dep_dioxygenase.
IPR006620. Pro_4_hyd_alph.
IPR013547. Pro_4_hyd_alph_N.
IPR013026. TPR-contain_dom.
IPR011990. TPR-like_helical_dom.
IPR019734. TPR_repeat.
[Graphical view]
PfamiPF13640. 2OG-FeII_Oxy_3. 1 hit.
PF08336. P4Ha_N. 1 hit.
[Graphical view]
SMARTiSM00702. P4Hc. 1 hit.
[Graphical view]
SUPFAMiSSF48452. SSF48452. 1 hit.
PROSITEiPS51471. FE2OG_OXY. 1 hit.
PS50005. TPR. 1 hit.
PS50293. TPR_REGION. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P54001-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIWGVLMMGI LLPQCSAHPG FFTSIGQMTD LIHNEKDLVT SLKDYIKAEE
60 70 80 90 100
DKLEQIKKWA EKLDRLTSTA TKDPEGFVGH PVNAFKLMKR LNTEWSELEN
110 120 130 140 150
LILKDMSDGF ISNLTIQRQY FPNDEDQVGA AKALFRLQDT YNLDTNTISK
160 170 180 190 200
GNLPGVKHKS FLTAEDCFEL GKVAYTEADY YHTELWMEQA LMQLEEGEMS
210 220 230 240 250
TVDKVSVLDY LSYAVYQQGD LDKALLLTKK LLELDPEHQR ANGNLVYFEY
260 270 280 290 300
IMSKEKDANK SASGDQSDQK TTPKKKGIAV DYLPERQKYE MLCRGEGIKM
310 320 330 340 350
TPRRQKRLFC RYHDGNRNPK FILAPAKQED EWDKPRIIRF HDIISDAEIE
360 370 380 390 400
IVKDLAKPRL SRATVHDPET GKLTTAQYRV SKSAWLSGYE DPVVSRINMR
410 420 430 440 450
IQDLTGLDVS TAEELQVANY GVGGQYEPHF DFARKDEPDA FRELGTGNRI
460 470 480 490 500
ATWLFYMSDV SAGGATVFPE VGASVWPKKG TAVFWYNLFA SGEGDYSTRH
510 520 530
AACPVLVGNK WVSNKWLHER GQEFRRPCTL SELE
Length:534
Mass (Da):60,898
Last modified:September 13, 2005 - v2
Checksum:iC44659978281FF30
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti265 – 2673DQS → ERA in CAA55546 (PubMed:7959029).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X78949 mRNA. Translation: CAA55546.1.
BC078703 mRNA. Translation: AAH78703.1.
PIRiS44204.
RefSeqiNP_742059.2. NM_172062.2.
XP_006256486.1. XM_006256424.2.
UniGeneiRn.8531.

Genome annotation databases

EnsembliENSRNOT00000070897; ENSRNOP00000064405; ENSRNOG00000050655.
GeneIDi64475.
KEGGirno:64475.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X78949 mRNA. Translation: CAA55546.1.
BC078703 mRNA. Translation: AAH78703.1.
PIRiS44204.
RefSeqiNP_742059.2. NM_172062.2.
XP_006256486.1. XM_006256424.2.
UniGeneiRn.8531.

3D structure databases

ProteinModelPortaliP54001.
SMRiP54001. Positions 161-254.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP54001. 1 interaction.
STRINGi10116.ENSRNOP00000064405.

Chemistry

BindingDBiP54001.
ChEMBLiCHEMBL3301.

Proteomic databases

PaxDbiP54001.
PRIDEiP54001.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000070897; ENSRNOP00000064405; ENSRNOG00000050655.
GeneIDi64475.
KEGGirno:64475.

Organism-specific databases

CTDi5033.
RGDi621000. P4ha1.

Phylogenomic databases

eggNOGiKOG1591. Eukaryota.
ENOG410XS5J. LUCA.
GeneTreeiENSGT00390000018885.
HOVERGENiHBG006834.
InParanoidiP54001.
KOiK00472.
OMAiFLTVEDC.
OrthoDBiEOG7W6WKC.
PhylomeDBiP54001.

Enzyme and pathway databases

BRENDAi1.14.11.2. 5301.
ReactomeiR-RNO-1650814. Collagen biosynthesis and modifying enzymes.

Miscellaneous databases

PROiP54001.

Family and domain databases

Gene3Di1.25.40.10. 2 hits.
InterProiIPR005123. Oxoglu/Fe-dep_dioxygenase.
IPR006620. Pro_4_hyd_alph.
IPR013547. Pro_4_hyd_alph_N.
IPR013026. TPR-contain_dom.
IPR011990. TPR-like_helical_dom.
IPR019734. TPR_repeat.
[Graphical view]
PfamiPF13640. 2OG-FeII_Oxy_3. 1 hit.
PF08336. P4Ha_N. 1 hit.
[Graphical view]
SMARTiSM00702. P4Hc. 1 hit.
[Graphical view]
SUPFAMiSSF48452. SSF48452. 1 hit.
PROSITEiPS51471. FE2OG_OXY. 1 hit.
PS50005. TPR. 1 hit.
PS50293. TPR_REGION. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The complete cDNA derived sequence of the rat prolyl 4-hydroxylase alpha subunit."
    Hopkinson I., Smith S.A., Donne A., Gregory H., Franklin T.J., Grant M.E., Rosamond J.
    Gene 149:391-392(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Testis.

Entry informationi

Entry nameiP4HA1_RAT
AccessioniPrimary (citable) accession number: P54001
Secondary accession number(s): Q6AZ74
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: September 13, 2005
Last modified: June 8, 2016
This is version 114 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.