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Protein

RNA polymerase II transcriptional coactivator SUB1

Gene

SUB1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plays a role in the release of TFIIB from the transcription complex during transcription initiation. Binds to TFIIB and specifically inhibits the formation of the TBP-TFIIB-promoter complexes.

GO - Molecular functioni

  • chromatin binding Source: SGD
  • DNA binding Source: UniProtKB-KW
  • transcription coactivator activity Source: SGD

GO - Biological processi

  • double-strand break repair via nonhomologous end joining Source: SGD
  • hyperosmotic response Source: SGD
  • negative regulation of ascospore formation Source: SGD
  • positive regulation of transcription elongation from RNA polymerase II promoter Source: SGD
  • positive regulation of transcription from RNA polymerase III promoter Source: SGD
  • positive regulation of transcription from RNA polymerase II promoter Source: SGD
  • regulation of transcription from RNA polymerase II promoter in response to stress Source: SGD
  • RNA polymerase III transcriptional preinitiation complex assembly Source: SGD
  • termination of RNA polymerase II transcription Source: SGD
Complete GO annotation...

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-32744-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
RNA polymerase II transcriptional coactivator SUB1
Gene namesi
Name:SUB1
Synonyms:TSP1
Ordered Locus Names:YMR039C
ORF Names:YM9532.04C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIII

Organism-specific databases

EuPathDBiFungiDB:YMR039C.
SGDiS000004642. SUB1.

Subcellular locationi

GO - Cellular componenti

  • nucleus Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 292292RNA polymerase II transcriptional coactivator SUB1PRO_0000215951Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei119 – 1191PhosphoserineCombined sources
Modified residuei268 – 2681PhosphoserineCombined sources
Modified residuei269 – 2691PhosphoserineCombined sources
Modified residuei289 – 2891PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP54000.

PTM databases

iPTMnetiP54000.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
RNA15P252992EBI-18492,EBI-15640

Protein-protein interaction databases

BioGridi35211. 184 interactions.
DIPiDIP-4696N.
IntActiP54000. 4 interactions.
MINTiMINT-540355.

Structurei

3D structure databases

ProteinModelPortaliP54000.
SMRiP54000. Positions 39-105.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00390000008802.
HOGENOMiHOG000000752.
InParanoidiP54000.
OMAiHEENIQN.
OrthoDBiEOG7BZW5P.

Family and domain databases

Gene3Di2.30.31.10. 1 hit.
InterProiIPR003173. PC4.
IPR009044. ssDNA-bd_transcriptional_reg.
[Graphical view]
PfamiPF02229. PC4. 1 hit.
[Graphical view]
SUPFAMiSSF54447. SSF54447. 1 hit.

Sequencei

Sequence statusi: Complete.

P54000-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSYYNRYRNK RRSDNGGGNL SNSNNNNGGM PSGLSASDAI FDLGKNKRVT
60 70 80 90 100
VRQFRNINLI DIREYYLDSS TGEMKPGKKG ISLTEDLYDE LLKHRLNIDE
110 120 130 140 150
ALRRLGSKRP KTKMVRLLSD DEYEDDNNND STNNDKDKNG KDKNSPKKRR
160 170 180 190 200
EDKSKASNES HDLEPRSKKK KPAPPTLLPH EENIQNAERE ANATLIIPGQ
210 220 230 240 250
AGRKQQEERK QKEKEEAEEA KAKAVAEQEK EAKAKEKIAE PEPEPVPTLQ
260 270 280 290
AKKEDIVSNI NESKDANSSD EEFAQSLEAE MNKAEDDISE EE
Length:292
Mass (Da):33,102
Last modified:October 1, 1996 - v1
Checksum:i1E278C3EF2D569C8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z48502 Genomic DNA. Translation: CAA88405.1.
BK006946 Genomic DNA. Translation: DAA09938.1.
PIRiS52888.
RefSeqiNP_013753.1. NM_001182536.1.

Genome annotation databases

EnsemblFungiiYMR039C; YMR039C; YMR039C.
GeneIDi855055.
KEGGisce:YMR039C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z48502 Genomic DNA. Translation: CAA88405.1.
BK006946 Genomic DNA. Translation: DAA09938.1.
PIRiS52888.
RefSeqiNP_013753.1. NM_001182536.1.

3D structure databases

ProteinModelPortaliP54000.
SMRiP54000. Positions 39-105.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi35211. 184 interactions.
DIPiDIP-4696N.
IntActiP54000. 4 interactions.
MINTiMINT-540355.

PTM databases

iPTMnetiP54000.

Proteomic databases

MaxQBiP54000.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYMR039C; YMR039C; YMR039C.
GeneIDi855055.
KEGGisce:YMR039C.

Organism-specific databases

EuPathDBiFungiDB:YMR039C.
SGDiS000004642. SUB1.

Phylogenomic databases

GeneTreeiENSGT00390000008802.
HOGENOMiHOG000000752.
InParanoidiP54000.
OMAiHEENIQN.
OrthoDBiEOG7BZW5P.

Enzyme and pathway databases

BioCyciYEAST:G3O-32744-MONOMER.

Miscellaneous databases

NextBioi978301.
PROiP54000.

Family and domain databases

Gene3Di2.30.31.10. 1 hit.
InterProiIPR003173. PC4.
IPR009044. ssDNA-bd_transcriptional_reg.
[Graphical view]
PfamiPF02229. PC4. 1 hit.
[Graphical view]
SUPFAMiSSF54447. SSF54447. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Yeast SUB1 is a suppressor of TFIIB mutations and has homology to the human co-activator PC4."
    Knaus R., Pollock R., Guarente L.
    EMBO J. 15:1933-1940(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  5. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-119; SER-268 AND SER-269, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  6. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-119 AND SER-289, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  7. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-119; SER-268; SER-269 AND SER-289, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiSUB1_YEAST
AccessioniPrimary (citable) accession number: P54000
Secondary accession number(s): D6VZL4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: May 11, 2016
This is version 131 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 7820 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.