RNA polymerase II transcriptional coactivator SUB1 - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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P54000 (SUB1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 93. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
RNA polymerase II transcriptional coactivator SUB1

Gene names

Name:	SUB1
Synonyms:	TSP1
Ordered Locus Names:	YMR039C
ORF Names:	YM9532.04C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	292 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Plays a role in the release of TFIIB from the transcription complex during transcription initiation. Binds to TFIIB and specifically inhibits the formation of the TBP-TFIIB-promoter complexes.
Subcellular location	Nucleus Probable.
Miscellaneous	Present with 7820 molecules/cell in log phase SD medium. Ref.4
Sequence similarities	Belongs to the transcriptional coactivator PC4 family.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Ligand	DNA-binding
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	RNA polymerase III transcriptional preinitiation complex assembly Inferred from direct assay. Source: SGD positive regulation of transcription elongation from RNA polymerase II promoter Inferred from mutant phenotype. Source: SGD positive regulation of transcription from RNA polymerase II promoter Inferred from physical interaction Ref.1. Source: SGD positive regulation of transcription from RNA polymerase III promoter Inferred from direct assay. Source: SGD regulation of transcription from RNA polymerase II promoter in response to stress Inferred from direct assay. Source: SGD termination of RNA polymerase II transcription Inferred from genetic interaction. Source: SGD
Cellular component	nucleus Inferred by curator Ref.1. Source: SGD
Molecular function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW protein binding Inferred from physical interaction. Source: IntAct transcription coactivator activity Inferred from physical interaction Ref.1. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
RNA15	P25299	2	EBI-18492,EBI-15640

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 292

292

RNA polymerase II transcriptional coactivator SUB1

PRO_0000215951

Amino acid modifications

Modified residue

119

Phosphoserine Ref.5 Ref.6 Ref.7

Modified residue

268

Phosphoserine Ref.5

Modified residue

269

Phosphoserine Ref.5

Modified residue

289

Phosphoserine Ref.7

Sequences

Sequence

Length

Mass (Da)

Tools

P54000 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: 1E278C3EF2D569C8
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FASTA

292

33,102

        10         20         30         40         50         60 
MSYYNRYRNK RRSDNGGGNL SNSNNNNGGM PSGLSASDAI FDLGKNKRVT VRQFRNINLI 

        70         80         90        100        110        120 
DIREYYLDSS TGEMKPGKKG ISLTEDLYDE LLKHRLNIDE ALRRLGSKRP KTKMVRLLSD 

       130        140        150        160        170        180 
DEYEDDNNND STNNDKDKNG KDKNSPKKRR EDKSKASNES HDLEPRSKKK KPAPPTLLPH 

       190        200        210        220        230        240 
EENIQNAERE ANATLIIPGQ AGRKQQEERK QKEKEEAEEA KAKAVAEQEK EAKAKEKIAE 

       250        260        270        280        290 
PEPEPVPTLQ AKKEDIVSNI NESKDANSSD EEFAQSLEAE MNKAEDDISE EE

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Yeast SUB1 is a suppressor of TFIIB mutations and has homology to the human co-activator PC4." Knaus R., Pollock R., Guarente L. EMBO J. 15:1933-1940(1996) [PubMed: 8617240] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII." Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. Barrell B.G. Nature 387:90-93(1997) [PubMed: 9169872] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[5]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed: 17330950] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-119; SER-268 AND SER-269, MASS SPECTROMETRY. Strain: ADR376.
[6]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed: 17563356] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-119, MASS SPECTROMETRY.
[7]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-119 AND SER-289, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z48502 Genomic DNA. Translation: CAA88405.1. BK006946 Genomic DNA. Translation: DAA09938.1.
PIR	S52888.
RefSeq	NP_013753.1. NM_001182536.1.
3D structure databases
ProteinModelPortal	P54000.
SMR	P54000. Positions 39-105.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-4696N.
IntAct	P54000. 2 interactions.
MINT	MINT-540355.
STRING	P54000.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YMR039C; YMR039C; YMR039C.
GeneID	855055.
KEGG	sce:YMR039C.
NMPDR	fig\|4932.3.peg.4800.
Organism-specific databases
CYGD	YMR039c.
SGD	S000004642. SUB1.
Phylogenomic databases
eggNOG	fuNOG12771.
GeneTree	EFGT00050000004998.
HOGENOM	HBG203392.
OMA	EREANAT.
OrthoDB	EOG4WQ4C5.
Gene expression databases
ArrayExpress	P54000.
Genevestigator	P54000.
GermOnline	YMR039C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR003173. PC4. IPR009044. ssDNA-bd_transcriptional_reg. [Graphical view]
Gene3D	G3DSA:2.30.31.10. ssDNA-bd_transcriptional_reg. 1 hit.
Pfam	PF02229. PC4. 1 hit. [Graphical view]
SUPFAM	SSF54447. ssDNA_bind_regul. 1 hit.
ProtoNet	Search...
Other
NextBio	978301.

Entry information

Entry name

SUB1_YEAST

Accession

Primary (citable) accession number: P54000
Secondary accession number(s): D6VZL4

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	January 25, 2012
This is version 93 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome XIII

Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents