P53996 (CNBP_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cellular nucleic acid-binding protein
Short name=CNBP

Alternative name(s):
Zinc finger protein 9

Gene names

Name:	Cnbp
Synonyms:	Cnbp1, Znf9

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	178 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Single stranded DNA-binding protein, with specificity to the sterol regulatory element (SRE). Involved in sterol-mediated repression.
Subcellular location	Cytoplasm. Endoplasmic reticulum.
Tissue specificity	Present in all tissues examined.
Sequence similarities	Contains 7 CCHC-type zinc fingers.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Cytoplasm Endoplasmic reticulum
Coding sequence diversity	Alternative splicing
Domain	Repeat Zinc-finger
Ligand	DNA-binding Metal-binding Zinc
Molecular function	Repressor
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	positive regulation of cell proliferation Inferred from direct assay Ref.3. Source: MGI positive regulation of transcription from RNA polymerase II promoter Inferred from direct assay Ref.3. Source: MGI transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytosol Inferred from direct assay Ref.1. Source: MGI endoplasmic reticulum Inferred from direct assay Ref.1. Source: MGI nucleus Inferred from direct assay Ref.3. Source: MGI
Molecular_function	single-stranded DNA binding Inferred from electronic annotation. Source: Compara single-stranded RNA binding Inferred from electronic annotation. Source: Compara zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: P53996-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: P53996-2)

The sequence of this isoform differs from the canonical sequence as follows:
     36-42: Missing.
     73-73: Missing.

Isoform 3 (identifier: P53996-3)

The sequence of this isoform differs from the canonical sequence as follows:
     73-73: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 178	178	Cellular nucleic acid-binding protein		PRO_0000089966
Regions
	Zinc finger	4 – 21	18	CCHC-type 1
	Zinc finger	52 – 69	18	CCHC-type 2
	Zinc finger	72 – 90	19	CCHC-type 3
	Zinc finger	97 – 114	18	CCHC-type 4
	Zinc finger	118 – 135	18	CCHC-type 5
	Zinc finger	136 – 153	18	CCHC-type 6
	Zinc finger	157 – 174	18	CCHC-type 7
Natural variations
	Alternative sequence	36 – 42	7	Missing in isoform 2.		VSP_010983
	Alternative sequence	73	1	Missing in isoform 2 and isoform 3.		VSP_010984
Experimental info
	Sequence conflict	2	1	S → R in CAA45345. Ref.1
	Sequence conflict	2	1	S → R in CAA77896. Ref.1
	Sequence conflict	60	1	S → P in BAC37269. Ref.4
	Sequence conflict	106	1	G → D in CAA77897. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified July 19, 2004. Version 2. Checksum: DF0CDAB9BF3D96BB	FASTA	178	19,592
	10 20 30 40 50 60 MSSNECFKCG RSGHWARECP TGGGRGRGMR SRGRGGFTSD RGFQFVSSSL PDICYRCGES 70 80 90 100 110 120 GHLAKDCDLQ EDEACYNCGR GGHIAKDCKE PKREREQCCY NCGKPGHLAR DCDHADEQKC 130 140 150 160 170 YSCGEFGHIQ KDCTKVKCYR CGETGHVAIN CSKTSEVNCY RCGESGHLAR ECTIEATA
	Isoform 2 [UniParc]. Checksum: 152BEC42881358E8	FASTA	170	18,742
	10 20 30 40 50 60 MSSNECFKCG RSGHWARECP TGGGRGRGMR SRGRGFQFVS SSLPDICYRC GESGHLAKDC 70 80 90 100 110 120 DLQEDACYNC GRGGHIAKDC KEPKREREQC CYNCGKPGHL ARDCDHADEQ KCYSCGEFGH 130 140 150 160 170 IQKDCTKVKC YRCGETGHVA INCSKTSEVN CYRCGESGHL ARECTIEATA
	Isoform 3 [UniParc]. Checksum: 996F398285F52618	FASTA	177	19,463
	10 20 30 40 50 60 MSSNECFKCG RSGHWARECP TGGGRGRGMR SRGRGGFTSD RGFQFVSSSL PDICYRCGES 70 80 90 100 110 120 GHLAKDCDLQ EDACYNCGRG GHIAKDCKEP KREREQCCYN CGKPGHLARD CDHADEQKCY 130 140 150 160 170 SCGEFGHIQK DCTKVKCYRC GETGHVAINC SKTSEVNCYR CGESGHLARE CTIEATA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Mouse cellular nucleic acid binding proteins: a highly conserved family identified by genetic mapping and sequencing." Warden C.H., Krisans S.K., Purcell-Huynh D., Leete L.M., Daluiski A., Diep A., Taylor B.A., Lusis A.J. Genomics 24:14-19(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 2 AND 3). Strain: C57BL/6J X CBA/J. Tissue: Liver.
[2]	"Primary structure and developmental regulation of murine CNBP, a zinc finger-containing protein whose general structure is present in evolutionarily diverse eukaryotes." Kingsley P.D., Palis J. Submitted (JAN-1995) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE (ISOFORM 1). Strain: C57BL/6.
[3]	"Molecular cloning, developmental expression, promoter analysis and functional characterization of the mouse CNBP gene." Shimizu K., Chen W., Ashique A.M., Moroi R., Li Y.P. Gene 307:51-62(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM 1).
[4]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2 AND 3). Strain: C57BL/6J. Tissue: Testis.
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: C57BL/6. Tissue: Brain.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L12693 mRNA. Translation: AAA89198.1. Z11870 mRNA. Translation: CAA77896.1. X63866 mRNA. Translation: CAA45345.1. Z11871 mRNA. Translation: CAA77897.1. U20326 mRNA. Translation: AAB60490.1. AY176064 Genomic DNA. Translation: AAO31613.1. AK075760 mRNA. Translation: BAC35938.1. AK078427 mRNA. Translation: BAC37269.1. BC058723 mRNA. Translation: AAH58723.1.
IPI	IPI00137229. IPI00330767. IPI00454151.
PIR	I48297. I48298. I49259.
RefSeq	NP_001103215.1. NM_001109745.1. NP_038521.1. NM_013493.3.
UniGene	Mm.290251.
3D structure databases
ProteinModelPortal	P53996.
SMR	P53996. Positions 4-70, 75-113, 136-175.
ModBase	Search...
Protein-protein interaction databases
IntAct	P53996. 2 interactions.
PTM databases
PhosphoSite	P53996.
Proteomic databases
PaxDb	P53996.
PRIDE	P53996.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000032138; ENSMUSP00000032138; ENSMUSG00000030057. ENSMUST00000113619; ENSMUSP00000109249; ENSMUSG00000030057.
GeneID	12785.
KEGG	mmu:12785.
Organism-specific databases
CTD	7555.
MGI	MGI:88431. Cnbp.
Phylogenomic databases
eggNOG	COG5082.
HOGENOM	HOG000186262.
HOVERGEN	HBG000397.
InParanoid	P53996.
KO	K09250.
OMA	CPNGQGG.
Gene expression databases
ArrayExpress	P53996.
Bgee	P53996.
CleanEx	MM_CNBP.
Genevestigator	P53996.
GermOnline	ENSMUSG00000030057. Mus musculus.
Family and domain databases
Gene3D	4.10.60.10. 4 hits.
InterPro	IPR001878. Znf_CCHC. [Graphical view]
Pfam	PF00098. zf-CCHC. 7 hits. [Graphical view]
SMART	SM00343. ZnF_C2HC. 7 hits. [Graphical view]
SUPFAM	SSF57756. SSF57756. 4 hits.
PROSITE	PS50158. ZF_CCHC. 7 hits. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	CNBP. mouse.
NextBio	282182.
SOURCE	Search...

Entry information

Entry name

CNBP_MOUSE

Accession

Primary (citable) accession number: P53996
Secondary accession number(s): Q80Y06, Q8BP23

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	July 19, 2004
Last modified:	May 1, 2013
This is version 105 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program