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P53996 (CNBP_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 117. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Cellular nucleic acid-binding protein

Short name=CNBP
Alternative name(s):
Zinc finger protein 9
Gene names
Name:Cnbp
Synonyms:Cnbp1, Znf9
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length178 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Single-stranded DNA-binding protein, with specificity to the sterol regulatory element (SRE). Involved in sterol-mediated repression.

Subcellular location

Cytoplasm. Endoplasmic reticulum.

Tissue specificity

Present in all tissues examined.

Post-translational modification

Arginine methylation by PRMT1 in the Arg/Gly-rich region impedes RNA binding By similarity.

Sequence similarities

Contains 7 CCHC-type zinc fingers.

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: P53996-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: P53996-2)

The sequence of this isoform differs from the canonical sequence as follows:
     36-42: Missing.
     73-73: Missing.
Isoform 3 (identifier: P53996-3)

The sequence of this isoform differs from the canonical sequence as follows:
     73-73: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 178177Cellular nucleic acid-binding protein
PRO_0000089966

Regions

Zinc finger4 – 2118CCHC-type 1
Zinc finger52 – 6918CCHC-type 2
Zinc finger72 – 9019CCHC-type 3
Zinc finger97 – 11418CCHC-type 4
Zinc finger118 – 13518CCHC-type 5
Zinc finger136 – 15318CCHC-type 6
Zinc finger157 – 17418CCHC-type 7
Compositional bias22 – 4221Arg/Gly-rich

Amino acid modifications

Modified residue21N-acetylserine Ref.6
Modified residue81N6-acetyllysine Ref.6
Modified residue251Omega-N-methylarginine; by PRMT1 By similarity
Modified residue271Omega-N-methylarginine; by PRMT1 By similarity

Natural variations

Alternative sequence36 – 427Missing in isoform 2.
VSP_010983
Alternative sequence731Missing in isoform 2 and isoform 3.
VSP_010984

Experimental info

Sequence conflict21S → R in CAA45345. Ref.1
Sequence conflict21S → R in CAA77896. Ref.1
Sequence conflict601S → P in BAC37269. Ref.4
Sequence conflict1061G → D in CAA77897. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified July 19, 2004. Version 2.
Checksum: DF0CDAB9BF3D96BB

FASTA17819,592
        10         20         30         40         50         60 
MSSNECFKCG RSGHWARECP TGGGRGRGMR SRGRGGFTSD RGFQFVSSSL PDICYRCGES 

        70         80         90        100        110        120 
GHLAKDCDLQ EDEACYNCGR GGHIAKDCKE PKREREQCCY NCGKPGHLAR DCDHADEQKC 

       130        140        150        160        170 
YSCGEFGHIQ KDCTKVKCYR CGETGHVAIN CSKTSEVNCY RCGESGHLAR ECTIEATA 

« Hide

Isoform 2 [UniParc].

Checksum: 152BEC42881358E8
Show »

FASTA17018,742
Isoform 3 [UniParc].

Checksum: 996F398285F52618
Show »

FASTA17719,463

References

« Hide 'large scale' references
[1]"Mouse cellular nucleic acid binding proteins: a highly conserved family identified by genetic mapping and sequencing."
Warden C.H., Krisans S.K., Purcell-Huynh D., Leete L.M., Daluiski A., Diep A., Taylor B.A., Lusis A.J.
Genomics 24:14-19(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 2 AND 3).
Strain: C57BL/6J X CBA/J.
Tissue: Liver.
[2]"Primary structure and developmental regulation of murine CNBP, a zinc finger-containing protein whose general structure is present in evolutionarily diverse eukaryotes."
Kingsley P.D., Palis J.
Submitted (JAN-1995) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE (ISOFORM 1).
Strain: C57BL/6.
[3]"Molecular cloning, developmental expression, promoter analysis and functional characterization of the mouse CNBP gene."
Shimizu K., Chen W., Ashique A.M., Moroi R., Li Y.P.
Gene 307:51-62(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM 1).
[4]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2 AND 3).
Strain: C57BL/6J.
Tissue: Testis.
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Strain: C57BL/6.
Tissue: Brain.
[6]"SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways."
Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y., Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.
Mol. Cell 50:919-930(2013) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2 AND LYS-8, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].
Tissue: Embryonic fibroblast.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L12693 mRNA. Translation: AAA89198.1.
Z11870 mRNA. Translation: CAA77896.1.
X63866 mRNA. Translation: CAA45345.1.
Z11871 mRNA. Translation: CAA77897.1.
U20326 mRNA. Translation: AAB60490.1.
AY176064 Genomic DNA. Translation: AAO31613.1.
AK075760 mRNA. Translation: BAC35938.1.
AK078427 mRNA. Translation: BAC37269.1.
BC058723 mRNA. Translation: AAH58723.1.
CCDSCCDS51841.1. [P53996-1]
PIRI48297.
I48298.
I49259.
RefSeqNP_001103215.1. NM_001109745.1.
NP_038521.1. NM_013493.3. [P53996-1]
XP_006505542.1. XM_006505479.1. [P53996-3]
UniGeneMm.290251.

3D structure databases

ProteinModelPortalP53996.
SMRP53996. Positions 4-70, 75-113, 136-175.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid198782. 2 interactions.
IntActP53996. 3 interactions.
MINTMINT-4091236.

PTM databases

PhosphoSiteP53996.

Proteomic databases

MaxQBP53996.
PaxDbP53996.
PRIDEP53996.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000032138; ENSMUSP00000032138; ENSMUSG00000030057. [P53996-1]
ENSMUST00000113619; ENSMUSP00000109249; ENSMUSG00000030057. [P53996-3]
GeneID12785.
KEGGmmu:12785.
UCSCuc009cuc.2. mouse. [P53996-1]

Organism-specific databases

CTD7555.
MGIMGI:88431. Cnbp.

Phylogenomic databases

eggNOGCOG5082.
HOGENOMHOG000186262.
HOVERGENHBG000397.
InParanoidP53996.
KOK09250.
OMACPNGQGG.
OrthoDBEOG790G22.
PhylomeDBP53996.
TreeFamTF316974.

Gene expression databases

ArrayExpressP53996.
BgeeP53996.
CleanExMM_CNBP.
GenevestigatorP53996.

Family and domain databases

Gene3D4.10.60.10. 4 hits.
InterProIPR001878. Znf_CCHC.
[Graphical view]
PfamPF00098. zf-CCHC. 7 hits.
[Graphical view]
SMARTSM00343. ZnF_C2HC. 7 hits.
[Graphical view]
SUPFAMSSF57756. SSF57756. 4 hits.
PROSITEPS50158. ZF_CCHC. 7 hits.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSCNBP. mouse.
NextBio282182.
PROP53996.
SOURCESearch...

Entry information

Entry nameCNBP_MOUSE
AccessionPrimary (citable) accession number: P53996
Secondary accession number(s): Q80Y06, Q8BP23
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: July 19, 2004
Last modified: July 9, 2014
This is version 117 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot