Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P53996

- CNBP_MOUSE

UniProt

P53996 - CNBP_MOUSE

Protein

Cellular nucleic acid-binding protein

Gene

Cnbp

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 118 (01 Oct 2014)
      Sequence version 2 (19 Jul 2004)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Single-stranded DNA-binding protein, with specificity to the sterol regulatory element (SRE). Involved in sterol-mediated repression.

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri4 – 2118CCHC-type 1PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri52 – 6918CCHC-type 2PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri72 – 9019CCHC-type 3PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri97 – 11418CCHC-type 4PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri118 – 13518CCHC-type 5PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri136 – 15318CCHC-type 6PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri157 – 17418CCHC-type 7PROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. single-stranded DNA binding Source: Ensembl
    2. single-stranded RNA binding Source: Ensembl
    3. zinc ion binding Source: InterPro

    GO - Biological processi

    1. positive regulation of cell proliferation Source: MGI
    2. positive regulation of transcription, DNA-templated Source: UniProtKB
    3. positive regulation of transcription from RNA polymerase II promoter Source: MGI
    4. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Molecular functioni

    Repressor

    Keywords - Biological processi

    Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding, Metal-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cellular nucleic acid-binding protein
    Short name:
    CNBP
    Alternative name(s):
    Zinc finger protein 9
    Gene namesi
    Name:Cnbp
    Synonyms:Cnbp1, Znf9
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 6

    Organism-specific databases

    MGIiMGI:88431. Cnbp.

    Subcellular locationi

    GO - Cellular componenti

    1. cytosol Source: MGI
    2. endoplasmic reticulum Source: MGI
    3. nucleus Source: MGI

    Keywords - Cellular componenti

    Cytoplasm, Endoplasmic reticulum

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 178177Cellular nucleic acid-binding proteinPRO_0000089966Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylserine1 Publication
    Modified residuei8 – 81N6-acetyllysine1 Publication
    Modified residuei25 – 251Omega-N-methylarginine; by PRMT1By similarity
    Modified residuei27 – 271Omega-N-methylarginine; by PRMT1By similarity

    Post-translational modificationi

    Arginine methylation by PRMT1 in the Arg/Gly-rich region impedes RNA binding.By similarity

    Keywords - PTMi

    Acetylation, Methylation

    Proteomic databases

    MaxQBiP53996.
    PaxDbiP53996.
    PRIDEiP53996.

    PTM databases

    PhosphoSiteiP53996.

    Expressioni

    Tissue specificityi

    Present in all tissues examined.

    Gene expression databases

    ArrayExpressiP53996.
    BgeeiP53996.
    CleanExiMM_CNBP.
    GenevestigatoriP53996.

    Interactioni

    Protein-protein interaction databases

    BioGridi198782. 2 interactions.
    IntActiP53996. 3 interactions.
    MINTiMINT-4091236.

    Structurei

    3D structure databases

    ProteinModelPortaliP53996.
    SMRiP53996. Positions 4-70, 75-113, 136-175.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi22 – 4221Arg/Gly-richAdd
    BLAST

    Sequence similaritiesi

    Contains 7 CCHC-type zinc fingers.PROSITE-ProRule annotation

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri4 – 2118CCHC-type 1PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri52 – 6918CCHC-type 2PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri72 – 9019CCHC-type 3PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri97 – 11418CCHC-type 4PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri118 – 13518CCHC-type 5PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri136 – 15318CCHC-type 6PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri157 – 17418CCHC-type 7PROSITE-ProRule annotationAdd
    BLAST

    Keywords - Domaini

    Repeat, Zinc-finger

    Phylogenomic databases

    eggNOGiCOG5082.
    HOGENOMiHOG000186262.
    HOVERGENiHBG000397.
    InParanoidiP53996.
    KOiK09250.
    OMAiCPNGQGG.
    OrthoDBiEOG790G22.
    PhylomeDBiP53996.
    TreeFamiTF316974.

    Family and domain databases

    Gene3Di4.10.60.10. 4 hits.
    InterProiIPR001878. Znf_CCHC.
    [Graphical view]
    PfamiPF00098. zf-CCHC. 7 hits.
    [Graphical view]
    SMARTiSM00343. ZnF_C2HC. 7 hits.
    [Graphical view]
    SUPFAMiSSF57756. SSF57756. 4 hits.
    PROSITEiPS50158. ZF_CCHC. 7 hits.
    [Graphical view]

    Sequences (3)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 3 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: P53996-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MSSNECFKCG RSGHWARECP TGGGRGRGMR SRGRGGFTSD RGFQFVSSSL    50
    PDICYRCGES GHLAKDCDLQ EDEACYNCGR GGHIAKDCKE PKREREQCCY 100
    NCGKPGHLAR DCDHADEQKC YSCGEFGHIQ KDCTKVKCYR CGETGHVAIN 150
    CSKTSEVNCY RCGESGHLAR ECTIEATA 178
    Length:178
    Mass (Da):19,592
    Last modified:July 19, 2004 - v2
    Checksum:iDF0CDAB9BF3D96BB
    GO
    Isoform 2 (identifier: P53996-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         36-42: Missing.
         73-73: Missing.

    Show »
    Length:170
    Mass (Da):18,742
    Checksum:i152BEC42881358E8
    GO
    Isoform 3 (identifier: P53996-3) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         73-73: Missing.

    Show »
    Length:177
    Mass (Da):19,463
    Checksum:i996F398285F52618
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti2 – 21S → R in CAA45345. (PubMed:7896269)Curated
    Sequence conflicti2 – 21S → R in CAA77896. (PubMed:7896269)Curated
    Sequence conflicti60 – 601S → P in BAC37269. (PubMed:16141072)Curated
    Sequence conflicti106 – 1061G → D in CAA77897. (PubMed:7896269)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei36 – 427Missing in isoform 2. 2 PublicationsVSP_010983
    Alternative sequencei73 – 731Missing in isoform 2 and isoform 3. 2 PublicationsVSP_010984

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L12693 mRNA. Translation: AAA89198.1.
    Z11870 mRNA. Translation: CAA77896.1.
    X63866 mRNA. Translation: CAA45345.1.
    Z11871 mRNA. Translation: CAA77897.1.
    U20326 mRNA. Translation: AAB60490.1.
    AY176064 Genomic DNA. Translation: AAO31613.1.
    AK075760 mRNA. Translation: BAC35938.1.
    AK078427 mRNA. Translation: BAC37269.1.
    BC058723 mRNA. Translation: AAH58723.1.
    CCDSiCCDS51841.1. [P53996-1]
    PIRiI48297.
    I48298.
    I49259.
    RefSeqiNP_001103215.1. NM_001109745.1.
    NP_038521.1. NM_013493.3. [P53996-1]
    XP_006505542.1. XM_006505479.1. [P53996-3]
    UniGeneiMm.290251.

    Genome annotation databases

    EnsembliENSMUST00000032138; ENSMUSP00000032138; ENSMUSG00000030057. [P53996-1]
    ENSMUST00000113619; ENSMUSP00000109249; ENSMUSG00000030057. [P53996-3]
    GeneIDi12785.
    KEGGimmu:12785.
    UCSCiuc009cuc.2. mouse. [P53996-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L12693 mRNA. Translation: AAA89198.1 .
    Z11870 mRNA. Translation: CAA77896.1 .
    X63866 mRNA. Translation: CAA45345.1 .
    Z11871 mRNA. Translation: CAA77897.1 .
    U20326 mRNA. Translation: AAB60490.1 .
    AY176064 Genomic DNA. Translation: AAO31613.1 .
    AK075760 mRNA. Translation: BAC35938.1 .
    AK078427 mRNA. Translation: BAC37269.1 .
    BC058723 mRNA. Translation: AAH58723.1 .
    CCDSi CCDS51841.1. [P53996-1 ]
    PIRi I48297.
    I48298.
    I49259.
    RefSeqi NP_001103215.1. NM_001109745.1.
    NP_038521.1. NM_013493.3. [P53996-1 ]
    XP_006505542.1. XM_006505479.1. [P53996-3 ]
    UniGenei Mm.290251.

    3D structure databases

    ProteinModelPortali P53996.
    SMRi P53996. Positions 4-70, 75-113, 136-175.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 198782. 2 interactions.
    IntActi P53996. 3 interactions.
    MINTi MINT-4091236.

    PTM databases

    PhosphoSitei P53996.

    Proteomic databases

    MaxQBi P53996.
    PaxDbi P53996.
    PRIDEi P53996.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000032138 ; ENSMUSP00000032138 ; ENSMUSG00000030057 . [P53996-1 ]
    ENSMUST00000113619 ; ENSMUSP00000109249 ; ENSMUSG00000030057 . [P53996-3 ]
    GeneIDi 12785.
    KEGGi mmu:12785.
    UCSCi uc009cuc.2. mouse. [P53996-1 ]

    Organism-specific databases

    CTDi 7555.
    MGIi MGI:88431. Cnbp.

    Phylogenomic databases

    eggNOGi COG5082.
    HOGENOMi HOG000186262.
    HOVERGENi HBG000397.
    InParanoidi P53996.
    KOi K09250.
    OMAi CPNGQGG.
    OrthoDBi EOG790G22.
    PhylomeDBi P53996.
    TreeFami TF316974.

    Miscellaneous databases

    ChiTaRSi CNBP. mouse.
    NextBioi 282182.
    PROi P53996.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P53996.
    Bgeei P53996.
    CleanExi MM_CNBP.
    Genevestigatori P53996.

    Family and domain databases

    Gene3Di 4.10.60.10. 4 hits.
    InterProi IPR001878. Znf_CCHC.
    [Graphical view ]
    Pfami PF00098. zf-CCHC. 7 hits.
    [Graphical view ]
    SMARTi SM00343. ZnF_C2HC. 7 hits.
    [Graphical view ]
    SUPFAMi SSF57756. SSF57756. 4 hits.
    PROSITEi PS50158. ZF_CCHC. 7 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Mouse cellular nucleic acid binding proteins: a highly conserved family identified by genetic mapping and sequencing."
      Warden C.H., Krisans S.K., Purcell-Huynh D., Leete L.M., Daluiski A., Diep A., Taylor B.A., Lusis A.J.
      Genomics 24:14-19(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 2 AND 3).
      Strain: C57BL/6J X CBA/J.
      Tissue: Liver.
    2. "Primary structure and developmental regulation of murine CNBP, a zinc finger-containing protein whose general structure is present in evolutionarily diverse eukaryotes."
      Kingsley P.D., Palis J.
      Submitted (JAN-1995) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE (ISOFORM 1).
      Strain: C57BL/6.
    3. "Molecular cloning, developmental expression, promoter analysis and functional characterization of the mouse CNBP gene."
      Shimizu K., Chen W., Ashique A.M., Moroi R., Li Y.P.
      Gene 307:51-62(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM 1).
    4. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2 AND 3).
      Strain: C57BL/6J.
      Tissue: Testis.
    5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Strain: C57BL/6.
      Tissue: Brain.
    6. "SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways."
      Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y., Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.
      Mol. Cell 50:919-930(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2 AND LYS-8, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].
      Tissue: Embryonic fibroblast.

    Entry informationi

    Entry nameiCNBP_MOUSE
    AccessioniPrimary (citable) accession number: P53996
    Secondary accession number(s): Q80Y06, Q8BP23
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: July 19, 2004
    Last modified: October 1, 2014
    This is version 118 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3