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Protein

Cellular nucleic acid-binding protein

Gene

Cnbp

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Single-stranded DNA-binding protein, with specificity to the sterol regulatory element (SRE). Involved in sterol-mediated repression.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri4 – 21CCHC-type 1PROSITE-ProRule annotationAdd BLAST18
Zinc fingeri52 – 69CCHC-type 2PROSITE-ProRule annotationAdd BLAST18
Zinc fingeri72 – 90CCHC-type 3PROSITE-ProRule annotationAdd BLAST19
Zinc fingeri97 – 114CCHC-type 4PROSITE-ProRule annotationAdd BLAST18
Zinc fingeri118 – 135CCHC-type 5PROSITE-ProRule annotationAdd BLAST18
Zinc fingeri136 – 153CCHC-type 6PROSITE-ProRule annotationAdd BLAST18
Zinc fingeri157 – 174CCHC-type 7PROSITE-ProRule annotationAdd BLAST18

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-binding, Repressor
Biological processTranscription, Transcription regulation
LigandMetal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Cellular nucleic acid-binding protein
Short name:
CNBP
Alternative name(s):
Zinc finger protein 9
Gene namesi
Name:Cnbp
Synonyms:Cnbp1, Znf9
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 6

Organism-specific databases

MGIiMGI:88431. Cnbp.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Endoplasmic reticulum

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00000899662 – 178Cellular nucleic acid-binding proteinAdd BLAST177

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineCombined sources1
Modified residuei8N6-acetyllysineCombined sources1
Modified residuei25Omega-N-methylarginine; by PRMT1By similarity1
Modified residuei27Omega-N-methylarginine; by PRMT1By similarity1
Modified residuei49PhosphoserineCombined sources1
Modified residuei80Omega-N-methylarginineBy similarity1
Isoform 2 (identifier: P53996-2)
Modified residuei32Omega-N-methylarginineCombined sources1
Modified residuei34Omega-N-methylarginineCombined sources1
Modified residuei72Omega-N-methylarginineCombined sources1
Isoform 3 (identifier: P53996-3)
Modified residuei79Omega-N-methylarginineCombined sources1

Post-translational modificationi

Arginine methylation by PRMT1 in the Arg/Gly-rich region impedes RNA binding.By similarity

Keywords - PTMi

Acetylation, Methylation, Phosphoprotein

Proteomic databases

EPDiP53996.
PaxDbiP53996.
PeptideAtlasiP53996.
PRIDEiP53996.

PTM databases

iPTMnetiP53996.
PhosphoSitePlusiP53996.

Expressioni

Tissue specificityi

Present in all tissues examined.

Gene expression databases

BgeeiENSMUSG00000030057.
CleanExiMM_CNBP.
ExpressionAtlasiP53996. baseline and differential.
GenevisibleiP53996. MM.

Interactioni

Protein-protein interaction databases

BioGridi198782. 2 interactors.
IntActiP53996. 3 interactors.
MINTiMINT-4091236.
STRINGi10090.ENSMUSP00000032138.

Structurei

3D structure databases

ProteinModelPortaliP53996.
SMRiP53996.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi22 – 42Arg/Gly-richAdd BLAST21

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri4 – 21CCHC-type 1PROSITE-ProRule annotationAdd BLAST18
Zinc fingeri52 – 69CCHC-type 2PROSITE-ProRule annotationAdd BLAST18
Zinc fingeri72 – 90CCHC-type 3PROSITE-ProRule annotationAdd BLAST19
Zinc fingeri97 – 114CCHC-type 4PROSITE-ProRule annotationAdd BLAST18
Zinc fingeri118 – 135CCHC-type 5PROSITE-ProRule annotationAdd BLAST18
Zinc fingeri136 – 153CCHC-type 6PROSITE-ProRule annotationAdd BLAST18
Zinc fingeri157 – 174CCHC-type 7PROSITE-ProRule annotationAdd BLAST18

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG4400. Eukaryota.
COG5082. LUCA.
GeneTreeiENSGT00510000047065.
HOGENOMiHOG000186262.
HOVERGENiHBG000397.
InParanoidiP53996.
KOiK09250.
OMAiRRECDEK.
OrthoDBiEOG091G0LZK.
PhylomeDBiP53996.
TreeFamiTF316974.

Family and domain databases

Gene3Di4.10.60.10. 7 hits.
InterProiView protein in InterPro
IPR001878. Znf_CCHC.
IPR036875. Znf_CCHC_sf.
PfamiView protein in Pfam
PF00098. zf-CCHC. 7 hits.
SMARTiView protein in SMART
SM00343. ZnF_C2HC. 7 hits.
SUPFAMiSSF57756. SSF57756. 4 hits.
PROSITEiView protein in PROSITE
PS50158. ZF_CCHC. 7 hits.

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P53996-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSSNECFKCG RSGHWARECP TGGGRGRGMR SRGRGGFTSD RGFQFVSSSL
60 70 80 90 100
PDICYRCGES GHLAKDCDLQ EDEACYNCGR GGHIAKDCKE PKREREQCCY
110 120 130 140 150
NCGKPGHLAR DCDHADEQKC YSCGEFGHIQ KDCTKVKCYR CGETGHVAIN
160 170
CSKTSEVNCY RCGESGHLAR ECTIEATA
Length:178
Mass (Da):19,592
Last modified:July 19, 2004 - v2
Checksum:iDF0CDAB9BF3D96BB
GO
Isoform 2 (identifier: P53996-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     36-42: Missing.
     73-73: Missing.

Show »
Length:170
Mass (Da):18,742
Checksum:i152BEC42881358E8
GO
Isoform 3 (identifier: P53996-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     73-73: Missing.

Show »
Length:177
Mass (Da):19,463
Checksum:i996F398285F52618
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti2S → R in CAA45345 (PubMed:7896269).Curated1
Sequence conflicti2S → R in CAA77896 (PubMed:7896269).Curated1
Sequence conflicti60S → P in BAC37269 (PubMed:16141072).Curated1
Sequence conflicti106G → D in CAA77897 (PubMed:7896269).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_01098336 – 42Missing in isoform 2. 2 Publications7
Alternative sequenceiVSP_01098473Missing in isoform 2 and isoform 3. 2 Publications1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L12693 mRNA. Translation: AAA89198.1.
Z11870 mRNA. Translation: CAA77896.1.
X63866 mRNA. Translation: CAA45345.1.
Z11871 mRNA. Translation: CAA77897.1.
U20326 mRNA. Translation: AAB60490.1.
AY176064 Genomic DNA. Translation: AAO31613.1.
AK075760 mRNA. Translation: BAC35938.1.
AK078427 mRNA. Translation: BAC37269.1.
BC058723 mRNA. Translation: AAH58723.1.
CCDSiCCDS51841.1. [P53996-1]
CCDS85091.1. [P53996-3]
CCDS85092.1. [P53996-2]
PIRiI48297.
I48298.
I49259.
RefSeqiNP_001103215.1. NM_001109745.1.
NP_001334254.1. NM_001347325.1. [P53996-3]
NP_038521.1. NM_013493.3. [P53996-1]
UniGeneiMm.290251.

Genome annotation databases

EnsembliENSMUST00000032138; ENSMUSP00000032138; ENSMUSG00000030057. [P53996-1]
ENSMUST00000113617; ENSMUSP00000109247; ENSMUSG00000030057. [P53996-3]
ENSMUST00000113619; ENSMUSP00000109249; ENSMUSG00000030057. [P53996-2]
ENSMUST00000204653; ENSMUSP00000145274; ENSMUSG00000030057. [P53996-3]
GeneIDi12785.
KEGGimmu:12785.
UCSCiuc009cuc.2. mouse. [P53996-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiCNBP_MOUSE
AccessioniPrimary (citable) accession number: P53996
Secondary accession number(s): Q80Y06, Q8BP23
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: July 19, 2004
Last modified: October 25, 2017
This is version 143 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot