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P53995

- APC1_MOUSE

UniProt

P53995 - APC1_MOUSE

Protein

Anaphase-promoting complex subunit 1

Gene

Anapc1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 102 (01 Oct 2014)
      Sequence version 2 (27 Jul 2011)
      Previous versions | rss
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    Functioni

    Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains By similarity.By similarity

    Pathwayi

    GO - Molecular functioni

    1. protein binding Source: MGI

    GO - Biological processi

    1. mitotic nuclear division Source: UniProtKB-KW
    2. protein K11-linked ubiquitination Source: UniProtKB

    Keywords - Biological processi

    Cell cycle, Cell division, Mitosis, Ubl conjugation pathway

    Enzyme and pathway databases

    ReactomeiREACT_199115. Antigen processing: Ubiquitination & Proteasome degradation.
    REACT_205250. Phosphorylation of the APC/C.
    REACT_206033. Senescence-Associated Secretory Phenotype (SASP).
    REACT_207679. Separation of Sister Chromatids.
    REACT_210462. APC/C:Cdc20 mediated degradation of Cyclin B.
    REACT_215601. Conversion from APC/C:Cdc20 to APC/C:Cdh1 in late anaphase.
    REACT_219897. APC/C:Cdc20 mediated degradation of Securin.
    REACT_222875. APC/C:Cdc20 mediated degradation of mitotic proteins.
    REACT_225686. Autodegradation of Cdh1 by Cdh1:APC/C.
    REACT_226135. APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1.
    UniPathwayiUPA00143.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Anaphase-promoting complex subunit 1
    Short name:
    APC1
    Alternative name(s):
    Cyclosome subunit 1
    Mitotic checkpoint regulator
    Testis-specific gene 24 protein
    Gene namesi
    Name:Anapc1
    Synonyms:Mcpr, Tsg24
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 2

    Organism-specific databases

    MGIiMGI:103097. Anapc1.

    Subcellular locationi

    GO - Cellular componenti

    1. anaphase-promoting complex Source: UniProtKB
    2. nucleus Source: MGI

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 19441944Anaphase-promoting complex subunit 1PRO_0000215872Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei51 – 511PhosphoserineBy similarity
    Modified residuei60 – 601PhosphoserineBy similarity
    Modified residuei291 – 2911PhosphothreonineBy similarity
    Modified residuei341 – 3411PhosphoserineBy similarity
    Modified residuei355 – 3551PhosphoserineBy similarity
    Modified residuei362 – 3621PhosphoserineBy similarity
    Modified residuei373 – 3731PhosphoserineBy similarity
    Modified residuei377 – 3771PhosphoserineBy similarity
    Modified residuei537 – 5371PhosphothreonineBy similarity
    Modified residuei547 – 5471PhosphoserineBy similarity
    Modified residuei555 – 5551PhosphoserineBy similarity
    Modified residuei571 – 5711PhosphotyrosineBy similarity
    Modified residuei686 – 6861PhosphoserineBy similarity
    Modified residuei688 – 6881PhosphoserineBy similarity

    Post-translational modificationi

    Phosphorylated. Phosphorylation on Ser-355 occurs specifically during mitosis By similarity.By similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP53995.
    PaxDbiP53995.
    PRIDEiP53995.

    PTM databases

    PhosphoSiteiP53995.

    Expressioni

    Tissue specificityi

    Abundantly expressed in proliferating fibroblasts, juvenile testis, adult brain and epididymis.

    Developmental stagei

    Uniformly expressed throughout interphase of the cell cycle.

    Gene expression databases

    ArrayExpressiP53995.
    BgeeiP53995.
    CleanExiMM_ANAPC1.
    GenevestigatoriP53995.

    Interactioni

    Subunit structurei

    The APC/C is composed of at least 12 subunits.By similarity

    Protein-protein interaction databases

    BioGridi201352. 14 interactions.
    IntActiP53995. 1 interaction.
    MINTiMINT-4087721.
    STRINGi10090.ENSMUSP00000014499.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati1297 – 132529PC 1Add
    BLAST
    Repeati1366 – 140439PC 2Add
    BLAST
    Repeati1467 – 150135PC 3Add
    BLAST
    Repeati1520 – 155233PC 4Add
    BLAST

    Sequence similaritiesi

    Belongs to the APC1 family.Curated
    Contains 4 PC repeats.Curated

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiNOG316940.
    GeneTreeiENSGT00390000016757.
    HOGENOMiHOG000231934.
    HOVERGENiHBG045326.
    InParanoidiA2ATQ4.
    KOiK03348.
    OMAiLAWTRNF.
    OrthoDBiEOG77WWCQ.
    TreeFamiTF105441.

    Family and domain databases

    InterProiIPR024990. Apc1.
    IPR002015. Proteasome/cyclosome_rpt.
    [Graphical view]
    PANTHERiPTHR12827. PTHR12827. 1 hit.
    PfamiPF12859. Apc1. 1 hit.
    PF01851. PC_rep. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P53995-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSNFSEERAT MIAAGDLQEF VPFGRDHCKH HPNALNLQLR QLQPASELWS     50
    SDGAAGLVGS LQEVTIHEKQ KESWQLRKGV SEIGDAADYD EELYVAGNMV 100
    IWSKGSKSQA LAVYKAFTVD STVQQALWCD FIISQDKSEK IYKSHELEKC 150
    ICILQSSCMN MHSIDGKDYI ASLPFQVANV WATKYGLLFE RCSSSHEVPP 200
    SLPREPLPTM FSMLHPLDEI TPLVCKSGSL FGSSRVQYVV DPAVKIVFLN 250
    IDPSIVMTYD AVQNVHSVWT LRRVKPEEEN AVLKFPEQAG TLQNATTSSS 300
    LTAHLRSLSK GESPVASPFQ NYSSIHSQSR STSSPSLHSR SPSISNMAAL 350
    SRAHSPALGV HSFSGAQRFN LSSHSQSPKR HSISHSPSGS FNDSFLAPET 400
    EPIVPELCID HLWTETLPNI REKNSQASKV FITTDLCGQK FLCFLVEAQL 450
    QLRCVKFQES NDKTQLIFGS VTNIHAKDAA PVEKIHTMLV LEGNGNLVLY 500
    TGVVRVGKVF IPGLPAPSLT MSNMMPRPST PLDGVGTPKP LSKLLGSMDE 550
    VVLLSPVPEL RDSSKLNDSL YNEDCTFQQL GTYIHSVRDP VHNRVTLELS 600
    NGSMVRITIP EVATSELVQT CLQAIKFILP KEVAIQVLVK WYNVHSAPGG 650
    PSCHSEWSLF VICLLNMMGY NTDRLAWTRS FDFEGSLSPV IAPKKARPSD 700
    TGSDEDWEYL LNSEYHRNVE SHLLNKSLCL TALEVSNAKD EDFSQNLSLD 750
    SSTLLFAHIP AIFFVLHLVY EELKLNTLMG EGICSLIDLL VQLARDLKLD 800
    SYLDHYYRDS PTLVKTTGQV CTIDQGQMGF MHHPPFFTSE PPSIYQWVSS 850
    CLKGEGMPPY PYLPGICERS RLVVLSIALY TLGDESCVSD ETCQYLSKVT 900
    STPQKPQAEQ EENRFTFRHS ASVSVLAERL VVWMASVGFT LRDLETLPFG 950
    IALPIRDAIY HCREQPDSDW SEAVCLLIGR QDLSKQACEG NLPRGKSVLS 1000
    SEVSSGTEAE EEDDGMNDLN HEVMSLIWSE DLRVQDVRRL LQSAQPVRVN 1050
    VVQYPELSDH EFIEEKENRL LQLCQRTMAL PVGRGMFTLF SYHPVPTEPL 1100
    PVPKLNLTGR APPRNTTVDL NSGNIDVPPN MASWASFHNG VAAGLKIAPA 1150
    SQIDSAWIVY NKPKHAELAN EYAGFLMALG LNGHLTKLAT LNIHDYLTKG 1200
    HEMTSIGLLL GVSAAKLGTM DMSITRLLSI HVPALLPPTS TELDVPHNVQ 1250
    VAAVVGIGLV YQGTAHRHTA EVLLAEIGRP PGPEMEYCTD RESYSLAAGL 1300
    ALGMVCLGHG SNLIGMSDLN VPEQLYQYMV GGHRRFQTGM HREKHKSPSY 1350
    QIKEGDTINV DVTCPGATLA LAMIYLKTNN RSIADWLRAP DTMYLLDFVK 1400
    PEFLLLRTLA RCLILWDDIL PNSKWVDSNV PQIIRENSIS LSEIELPCSE 1450
    DLNLETLSQA HVYIIAGACL SLGFRFAGSE NLSAFSCLHK FAKDFMNYLS 1500
    APNASVTGPY NLETCLSVVL LSLAMVMAGS GNLKVLQLCR FLHMKTGGEM 1550
    NYGFHLAHHM ALGLLFLGGG RYSLSTSNSS IAALLCALYP HFPAHSTDNR 1600
    YHLQALRHLY VLAAEPRLLV PVDVDTNTPC YALIEVTYKG TQWYEQTKEE 1650
    LMAPTLLPEL HLLKQMKVKG PRYWELLIDL SKGEQHLRSI LSKDGVLYVK 1700
    LRAGQLSYKE DPMGWQSLLA QTVANRNSEA RAFKPETISS FTSDPALLSF 1750
    AEYFCKPTVS MGPKQEILDL FSSILYECVA QETPEMLPAY IAMDQALRSL 1800
    KKRDMSDTSD LWQIKLILEF FSSRSHQDRQ HTYPKRGLFI NSEFLPVVKC 1850
    TVDATLDQWL QAGGDVCVHA YLSGQPVEKS QLNMLACFLV YHSVPAPRHL 1900
    PPMGLEGSTS FAELLYRFRH LKMPVRALLR LAPVLLGNPQ PMVM 1944
    Length:1,944
    Mass (Da):215,994
    Last modified:July 27, 2011 - v2
    Checksum:i596DD3FC07CDA321
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti112 – 1121A → Q in CAA56450. (PubMed:7929068)Curated
    Sequence conflicti348 – 3492AA → GV in CAA56450. (PubMed:7929068)Curated
    Sequence conflicti643 – 6431N → K in BAC34976. (PubMed:16141072)Curated
    Sequence conflicti1036 – 10361D → H in CAA56450. (PubMed:7929068)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X80169 mRNA. Translation: CAA56450.1.
    AL928910 Genomic DNA. Translation: CAM25161.1.
    CH466519 Genomic DNA. Translation: EDL28216.1.
    BC139002 mRNA. Translation: AAI39003.1.
    AK052404 mRNA. Translation: BAC34976.1.
    AK077847 mRNA. Translation: BAC37032.2.
    CCDSiCCDS16715.1.
    PIRiA55117.
    RefSeqiNP_032595.2. NM_008569.2.
    UniGeneiMm.277408.
    Mm.449185.

    Genome annotation databases

    EnsembliENSMUST00000014499; ENSMUSP00000014499; ENSMUSG00000014355.
    GeneIDi17222.
    KEGGimmu:17222.
    UCSCiuc008mgq.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X80169 mRNA. Translation: CAA56450.1 .
    AL928910 Genomic DNA. Translation: CAM25161.1 .
    CH466519 Genomic DNA. Translation: EDL28216.1 .
    BC139002 mRNA. Translation: AAI39003.1 .
    AK052404 mRNA. Translation: BAC34976.1 .
    AK077847 mRNA. Translation: BAC37032.2 .
    CCDSi CCDS16715.1.
    PIRi A55117.
    RefSeqi NP_032595.2. NM_008569.2.
    UniGenei Mm.277408.
    Mm.449185.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 201352. 14 interactions.
    IntActi P53995. 1 interaction.
    MINTi MINT-4087721.
    STRINGi 10090.ENSMUSP00000014499.

    PTM databases

    PhosphoSitei P53995.

    Proteomic databases

    MaxQBi P53995.
    PaxDbi P53995.
    PRIDEi P53995.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000014499 ; ENSMUSP00000014499 ; ENSMUSG00000014355 .
    GeneIDi 17222.
    KEGGi mmu:17222.
    UCSCi uc008mgq.2. mouse.

    Organism-specific databases

    CTDi 64682.
    MGIi MGI:103097. Anapc1.

    Phylogenomic databases

    eggNOGi NOG316940.
    GeneTreei ENSGT00390000016757.
    HOGENOMi HOG000231934.
    HOVERGENi HBG045326.
    InParanoidi A2ATQ4.
    KOi K03348.
    OMAi LAWTRNF.
    OrthoDBi EOG77WWCQ.
    TreeFami TF105441.

    Enzyme and pathway databases

    UniPathwayi UPA00143 .
    Reactomei REACT_199115. Antigen processing: Ubiquitination & Proteasome degradation.
    REACT_205250. Phosphorylation of the APC/C.
    REACT_206033. Senescence-Associated Secretory Phenotype (SASP).
    REACT_207679. Separation of Sister Chromatids.
    REACT_210462. APC/C:Cdc20 mediated degradation of Cyclin B.
    REACT_215601. Conversion from APC/C:Cdc20 to APC/C:Cdh1 in late anaphase.
    REACT_219897. APC/C:Cdc20 mediated degradation of Securin.
    REACT_222875. APC/C:Cdc20 mediated degradation of mitotic proteins.
    REACT_225686. Autodegradation of Cdh1 by Cdh1:APC/C.
    REACT_226135. APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1.

    Miscellaneous databases

    NextBioi 291630.
    PROi P53995.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P53995.
    Bgeei P53995.
    CleanExi MM_ANAPC1.
    Genevestigatori P53995.

    Family and domain databases

    InterProi IPR024990. Apc1.
    IPR002015. Proteasome/cyclosome_rpt.
    [Graphical view ]
    PANTHERi PTHR12827. PTHR12827. 1 hit.
    Pfami PF12859. Apc1. 1 hit.
    PF01851. PC_rep. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "A novel murine gene encoding a 216-kDa protein is related to a mitotic checkpoint regulator previously identified in Aspergillus nidulans."
      Starborg M., Brundell E., Gell K., Hoeoeg C.
      J. Biol. Chem. 269:24133-24137(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: CBA.
      Tissue: Testis.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    3. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
      Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    5. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-1063.
      Strain: C57BL/6J.
      Tissue: Embryonic lung and Forelimb.

    Entry informationi

    Entry nameiAPC1_MOUSE
    AccessioniPrimary (citable) accession number: P53995
    Secondary accession number(s): A2ATQ4, Q8BP33, Q8C772
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: July 27, 2011
    Last modified: October 1, 2014
    This is version 102 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3