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P53995 (APC1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 97. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Anaphase-promoting complex subunit 1

Short name=APC1
Alternative name(s):
Cyclosome subunit 1
Mitotic checkpoint regulator
Testis-specific gene 24 protein
Gene names
Name:Anapc1
Synonyms:Mcpr, Tsg24
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length1944 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains By similarity.

Pathway

Protein modification; protein ubiquitination.

Subunit structure

The APC/C is composed of at least 12 subunits By similarity.

Tissue specificity

Abundantly expressed in proliferating fibroblasts, juvenile testis, adult brain and epididymis.

Developmental stage

Uniformly expressed throughout interphase of the cell cycle.

Post-translational modification

Phosphorylated. Phosphorylation on Ser-355 occurs specifically during mitosis By similarity.

Sequence similarities

Belongs to the APC1 family.

Contains 4 PC repeats.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 19441944Anaphase-promoting complex subunit 1
PRO_0000215872

Regions

Repeat1297 – 132529PC 1
Repeat1366 – 140439PC 2
Repeat1467 – 150135PC 3
Repeat1520 – 155233PC 4

Amino acid modifications

Modified residue511Phosphoserine By similarity
Modified residue601Phosphoserine By similarity
Modified residue2911Phosphothreonine By similarity
Modified residue3411Phosphoserine By similarity
Modified residue3551Phosphoserine By similarity
Modified residue3621Phosphoserine By similarity
Modified residue3731Phosphoserine By similarity
Modified residue3771Phosphoserine By similarity
Modified residue5371Phosphothreonine By similarity
Modified residue5471Phosphoserine By similarity
Modified residue5551Phosphoserine By similarity
Modified residue5711Phosphotyrosine By similarity
Modified residue6861Phosphoserine By similarity
Modified residue6881Phosphoserine By similarity

Experimental info

Sequence conflict1121A → Q in CAA56450. Ref.1
Sequence conflict348 – 3492AA → GV in CAA56450. Ref.1
Sequence conflict6431N → K in BAC34976. Ref.5
Sequence conflict10361D → H in CAA56450. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P53995 [UniParc].

Last modified July 27, 2011. Version 2.
Checksum: 596DD3FC07CDA321

FASTA1,944215,994
        10         20         30         40         50         60 
MSNFSEERAT MIAAGDLQEF VPFGRDHCKH HPNALNLQLR QLQPASELWS SDGAAGLVGS 

        70         80         90        100        110        120 
LQEVTIHEKQ KESWQLRKGV SEIGDAADYD EELYVAGNMV IWSKGSKSQA LAVYKAFTVD 

       130        140        150        160        170        180 
STVQQALWCD FIISQDKSEK IYKSHELEKC ICILQSSCMN MHSIDGKDYI ASLPFQVANV 

       190        200        210        220        230        240 
WATKYGLLFE RCSSSHEVPP SLPREPLPTM FSMLHPLDEI TPLVCKSGSL FGSSRVQYVV 

       250        260        270        280        290        300 
DPAVKIVFLN IDPSIVMTYD AVQNVHSVWT LRRVKPEEEN AVLKFPEQAG TLQNATTSSS 

       310        320        330        340        350        360 
LTAHLRSLSK GESPVASPFQ NYSSIHSQSR STSSPSLHSR SPSISNMAAL SRAHSPALGV 

       370        380        390        400        410        420 
HSFSGAQRFN LSSHSQSPKR HSISHSPSGS FNDSFLAPET EPIVPELCID HLWTETLPNI 

       430        440        450        460        470        480 
REKNSQASKV FITTDLCGQK FLCFLVEAQL QLRCVKFQES NDKTQLIFGS VTNIHAKDAA 

       490        500        510        520        530        540 
PVEKIHTMLV LEGNGNLVLY TGVVRVGKVF IPGLPAPSLT MSNMMPRPST PLDGVGTPKP 

       550        560        570        580        590        600 
LSKLLGSMDE VVLLSPVPEL RDSSKLNDSL YNEDCTFQQL GTYIHSVRDP VHNRVTLELS 

       610        620        630        640        650        660 
NGSMVRITIP EVATSELVQT CLQAIKFILP KEVAIQVLVK WYNVHSAPGG PSCHSEWSLF 

       670        680        690        700        710        720 
VICLLNMMGY NTDRLAWTRS FDFEGSLSPV IAPKKARPSD TGSDEDWEYL LNSEYHRNVE 

       730        740        750        760        770        780 
SHLLNKSLCL TALEVSNAKD EDFSQNLSLD SSTLLFAHIP AIFFVLHLVY EELKLNTLMG 

       790        800        810        820        830        840 
EGICSLIDLL VQLARDLKLD SYLDHYYRDS PTLVKTTGQV CTIDQGQMGF MHHPPFFTSE 

       850        860        870        880        890        900 
PPSIYQWVSS CLKGEGMPPY PYLPGICERS RLVVLSIALY TLGDESCVSD ETCQYLSKVT 

       910        920        930        940        950        960 
STPQKPQAEQ EENRFTFRHS ASVSVLAERL VVWMASVGFT LRDLETLPFG IALPIRDAIY 

       970        980        990       1000       1010       1020 
HCREQPDSDW SEAVCLLIGR QDLSKQACEG NLPRGKSVLS SEVSSGTEAE EEDDGMNDLN 

      1030       1040       1050       1060       1070       1080 
HEVMSLIWSE DLRVQDVRRL LQSAQPVRVN VVQYPELSDH EFIEEKENRL LQLCQRTMAL 

      1090       1100       1110       1120       1130       1140 
PVGRGMFTLF SYHPVPTEPL PVPKLNLTGR APPRNTTVDL NSGNIDVPPN MASWASFHNG 

      1150       1160       1170       1180       1190       1200 
VAAGLKIAPA SQIDSAWIVY NKPKHAELAN EYAGFLMALG LNGHLTKLAT LNIHDYLTKG 

      1210       1220       1230       1240       1250       1260 
HEMTSIGLLL GVSAAKLGTM DMSITRLLSI HVPALLPPTS TELDVPHNVQ VAAVVGIGLV 

      1270       1280       1290       1300       1310       1320 
YQGTAHRHTA EVLLAEIGRP PGPEMEYCTD RESYSLAAGL ALGMVCLGHG SNLIGMSDLN 

      1330       1340       1350       1360       1370       1380 
VPEQLYQYMV GGHRRFQTGM HREKHKSPSY QIKEGDTINV DVTCPGATLA LAMIYLKTNN 

      1390       1400       1410       1420       1430       1440 
RSIADWLRAP DTMYLLDFVK PEFLLLRTLA RCLILWDDIL PNSKWVDSNV PQIIRENSIS 

      1450       1460       1470       1480       1490       1500 
LSEIELPCSE DLNLETLSQA HVYIIAGACL SLGFRFAGSE NLSAFSCLHK FAKDFMNYLS 

      1510       1520       1530       1540       1550       1560 
APNASVTGPY NLETCLSVVL LSLAMVMAGS GNLKVLQLCR FLHMKTGGEM NYGFHLAHHM 

      1570       1580       1590       1600       1610       1620 
ALGLLFLGGG RYSLSTSNSS IAALLCALYP HFPAHSTDNR YHLQALRHLY VLAAEPRLLV 

      1630       1640       1650       1660       1670       1680 
PVDVDTNTPC YALIEVTYKG TQWYEQTKEE LMAPTLLPEL HLLKQMKVKG PRYWELLIDL 

      1690       1700       1710       1720       1730       1740 
SKGEQHLRSI LSKDGVLYVK LRAGQLSYKE DPMGWQSLLA QTVANRNSEA RAFKPETISS 

      1750       1760       1770       1780       1790       1800 
FTSDPALLSF AEYFCKPTVS MGPKQEILDL FSSILYECVA QETPEMLPAY IAMDQALRSL 

      1810       1820       1830       1840       1850       1860 
KKRDMSDTSD LWQIKLILEF FSSRSHQDRQ HTYPKRGLFI NSEFLPVVKC TVDATLDQWL 

      1870       1880       1890       1900       1910       1920 
QAGGDVCVHA YLSGQPVEKS QLNMLACFLV YHSVPAPRHL PPMGLEGSTS FAELLYRFRH 

      1930       1940 
LKMPVRALLR LAPVLLGNPQ PMVM 

« Hide

References

« Hide 'large scale' references
[1]"A novel murine gene encoding a 216-kDa protein is related to a mitotic checkpoint regulator previously identified in Aspergillus nidulans."
Starborg M., Brundell E., Gell K., Hoeoeg C.
J. Biol. Chem. 269:24133-24137(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: CBA.
Tissue: Testis.
[2]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[3]Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[5]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-1063.
Strain: C57BL/6J.
Tissue: Embryonic lung and Forelimb.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X80169 mRNA. Translation: CAA56450.1.
AL928910 Genomic DNA. Translation: CAM25161.1.
CH466519 Genomic DNA. Translation: EDL28216.1.
BC139002 mRNA. Translation: AAI39003.1.
AK052404 mRNA. Translation: BAC34976.1.
AK077847 mRNA. Translation: BAC37032.2.
PIRA55117.
RefSeqNP_032595.2. NM_008569.2.
UniGeneMm.277408.
Mm.449185.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid201352. 14 interactions.
IntActP53995. 1 interaction.
MINTMINT-4087721.
STRING10090.ENSMUSP00000014499.

PTM databases

PhosphoSiteP53995.

Proteomic databases

PaxDbP53995.
PRIDEP53995.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000014499; ENSMUSP00000014499; ENSMUSG00000014355.
GeneID17222.
KEGGmmu:17222.
UCSCuc008mgq.2. mouse.

Organism-specific databases

CTD64682.
MGIMGI:103097. Anapc1.

Phylogenomic databases

eggNOGNOG316940.
GeneTreeENSGT00390000016757.
HOGENOMHOG000231934.
HOVERGENHBG045326.
InParanoidA2ATQ4.
KOK03348.
OMALAWTRNF.
OrthoDBEOG77WWCQ.
TreeFamTF105441.

Enzyme and pathway databases

UniPathwayUPA00143.

Gene expression databases

ArrayExpressP53995.
BgeeP53995.
CleanExMM_ANAPC1.
GenevestigatorP53995.

Family and domain databases

InterProIPR024990. Apc1.
IPR002015. Proteasome/cyclosome_rpt.
[Graphical view]
PANTHERPTHR12827. PTHR12827. 1 hit.
PfamPF12859. Apc1. 1 hit.
PF01851. PC_rep. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio291630.
PROP53995.
SOURCESearch...

Entry information

Entry nameAPC1_MOUSE
AccessionPrimary (citable) accession number: P53995
Secondary accession number(s): A2ATQ4, Q8BP33, Q8C772
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: July 27, 2011
Last modified: April 16, 2014
This is version 97 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot