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P53995

- APC1_MOUSE

UniProt

P53995 - APC1_MOUSE

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Protein

Anaphase-promoting complex subunit 1

Gene

Anapc1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli

Functioni

Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains (By similarity).By similarity

Pathwayi

GO - Biological processi

  1. mitotic nuclear division Source: UniProtKB-KW
  2. protein K11-linked ubiquitination Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division, Mitosis, Ubl conjugation pathway

Enzyme and pathway databases

ReactomeiREACT_199115. Antigen processing: Ubiquitination & Proteasome degradation.
REACT_205250. Phosphorylation of the APC/C.
REACT_206033. Senescence-Associated Secretory Phenotype (SASP).
REACT_207679. Separation of Sister Chromatids.
REACT_210462. APC/C:Cdc20 mediated degradation of Cyclin B.
REACT_215601. Conversion from APC/C:Cdc20 to APC/C:Cdh1 in late anaphase.
REACT_219897. APC/C:Cdc20 mediated degradation of Securin.
REACT_222875. APC/C:Cdc20 mediated degradation of mitotic proteins.
REACT_225686. Autodegradation of Cdh1 by Cdh1:APC/C.
REACT_226135. APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1.
UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
Anaphase-promoting complex subunit 1
Short name:
APC1
Alternative name(s):
Cyclosome subunit 1
Mitotic checkpoint regulator
Testis-specific gene 24 protein
Gene namesi
Name:Anapc1
Synonyms:Mcpr, Tsg24
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 2

Organism-specific databases

MGIiMGI:103097. Anapc1.

Subcellular locationi

GO - Cellular componenti

  1. anaphase-promoting complex Source: UniProtKB
  2. nucleus Source: MGI
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 19441944Anaphase-promoting complex subunit 1PRO_0000215872Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei51 – 511PhosphoserineBy similarity
Modified residuei60 – 601PhosphoserineBy similarity
Modified residuei291 – 2911PhosphothreonineBy similarity
Modified residuei341 – 3411PhosphoserineBy similarity
Modified residuei355 – 3551PhosphoserineBy similarity
Modified residuei362 – 3621PhosphoserineBy similarity
Modified residuei373 – 3731PhosphoserineBy similarity
Modified residuei377 – 3771PhosphoserineBy similarity
Modified residuei537 – 5371PhosphothreonineBy similarity
Modified residuei547 – 5471PhosphoserineBy similarity
Modified residuei555 – 5551PhosphoserineBy similarity
Modified residuei571 – 5711PhosphotyrosineBy similarity
Modified residuei686 – 6861PhosphoserineBy similarity
Modified residuei688 – 6881PhosphoserineBy similarity

Post-translational modificationi

Phosphorylated. Phosphorylation on Ser-355 occurs specifically during mitosis (By similarity).By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP53995.
PaxDbiP53995.
PRIDEiP53995.

PTM databases

PhosphoSiteiP53995.

Expressioni

Tissue specificityi

Abundantly expressed in proliferating fibroblasts, juvenile testis, adult brain and epididymis.

Developmental stagei

Uniformly expressed throughout interphase of the cell cycle.

Gene expression databases

BgeeiP53995.
CleanExiMM_ANAPC1.
ExpressionAtlasiP53995. baseline and differential.
GenevestigatoriP53995.

Interactioni

Subunit structurei

The APC/C is composed of at least 12 subunits.By similarity

Protein-protein interaction databases

BioGridi201352. 14 interactions.
IntActiP53995. 1 interaction.
MINTiMINT-4087721.
STRINGi10090.ENSMUSP00000014499.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati1297 – 132529PC 1Add
BLAST
Repeati1366 – 140439PC 2Add
BLAST
Repeati1467 – 150135PC 3Add
BLAST
Repeati1520 – 155233PC 4Add
BLAST

Sequence similaritiesi

Belongs to the APC1 family.Curated
Contains 4 PC repeats.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiNOG316940.
GeneTreeiENSGT00390000016757.
HOGENOMiHOG000231934.
HOVERGENiHBG045326.
InParanoidiP53995.
KOiK03348.
OMAiLAWTRNF.
OrthoDBiEOG77WWCQ.
TreeFamiTF105441.

Family and domain databases

InterProiIPR024990. Apc1.
IPR002015. Proteasome/cyclosome_rpt.
[Graphical view]
PANTHERiPTHR12827. PTHR12827. 1 hit.
PfamiPF12859. Apc1. 1 hit.
PF01851. PC_rep. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P53995-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSNFSEERAT MIAAGDLQEF VPFGRDHCKH HPNALNLQLR QLQPASELWS
60 70 80 90 100
SDGAAGLVGS LQEVTIHEKQ KESWQLRKGV SEIGDAADYD EELYVAGNMV
110 120 130 140 150
IWSKGSKSQA LAVYKAFTVD STVQQALWCD FIISQDKSEK IYKSHELEKC
160 170 180 190 200
ICILQSSCMN MHSIDGKDYI ASLPFQVANV WATKYGLLFE RCSSSHEVPP
210 220 230 240 250
SLPREPLPTM FSMLHPLDEI TPLVCKSGSL FGSSRVQYVV DPAVKIVFLN
260 270 280 290 300
IDPSIVMTYD AVQNVHSVWT LRRVKPEEEN AVLKFPEQAG TLQNATTSSS
310 320 330 340 350
LTAHLRSLSK GESPVASPFQ NYSSIHSQSR STSSPSLHSR SPSISNMAAL
360 370 380 390 400
SRAHSPALGV HSFSGAQRFN LSSHSQSPKR HSISHSPSGS FNDSFLAPET
410 420 430 440 450
EPIVPELCID HLWTETLPNI REKNSQASKV FITTDLCGQK FLCFLVEAQL
460 470 480 490 500
QLRCVKFQES NDKTQLIFGS VTNIHAKDAA PVEKIHTMLV LEGNGNLVLY
510 520 530 540 550
TGVVRVGKVF IPGLPAPSLT MSNMMPRPST PLDGVGTPKP LSKLLGSMDE
560 570 580 590 600
VVLLSPVPEL RDSSKLNDSL YNEDCTFQQL GTYIHSVRDP VHNRVTLELS
610 620 630 640 650
NGSMVRITIP EVATSELVQT CLQAIKFILP KEVAIQVLVK WYNVHSAPGG
660 670 680 690 700
PSCHSEWSLF VICLLNMMGY NTDRLAWTRS FDFEGSLSPV IAPKKARPSD
710 720 730 740 750
TGSDEDWEYL LNSEYHRNVE SHLLNKSLCL TALEVSNAKD EDFSQNLSLD
760 770 780 790 800
SSTLLFAHIP AIFFVLHLVY EELKLNTLMG EGICSLIDLL VQLARDLKLD
810 820 830 840 850
SYLDHYYRDS PTLVKTTGQV CTIDQGQMGF MHHPPFFTSE PPSIYQWVSS
860 870 880 890 900
CLKGEGMPPY PYLPGICERS RLVVLSIALY TLGDESCVSD ETCQYLSKVT
910 920 930 940 950
STPQKPQAEQ EENRFTFRHS ASVSVLAERL VVWMASVGFT LRDLETLPFG
960 970 980 990 1000
IALPIRDAIY HCREQPDSDW SEAVCLLIGR QDLSKQACEG NLPRGKSVLS
1010 1020 1030 1040 1050
SEVSSGTEAE EEDDGMNDLN HEVMSLIWSE DLRVQDVRRL LQSAQPVRVN
1060 1070 1080 1090 1100
VVQYPELSDH EFIEEKENRL LQLCQRTMAL PVGRGMFTLF SYHPVPTEPL
1110 1120 1130 1140 1150
PVPKLNLTGR APPRNTTVDL NSGNIDVPPN MASWASFHNG VAAGLKIAPA
1160 1170 1180 1190 1200
SQIDSAWIVY NKPKHAELAN EYAGFLMALG LNGHLTKLAT LNIHDYLTKG
1210 1220 1230 1240 1250
HEMTSIGLLL GVSAAKLGTM DMSITRLLSI HVPALLPPTS TELDVPHNVQ
1260 1270 1280 1290 1300
VAAVVGIGLV YQGTAHRHTA EVLLAEIGRP PGPEMEYCTD RESYSLAAGL
1310 1320 1330 1340 1350
ALGMVCLGHG SNLIGMSDLN VPEQLYQYMV GGHRRFQTGM HREKHKSPSY
1360 1370 1380 1390 1400
QIKEGDTINV DVTCPGATLA LAMIYLKTNN RSIADWLRAP DTMYLLDFVK
1410 1420 1430 1440 1450
PEFLLLRTLA RCLILWDDIL PNSKWVDSNV PQIIRENSIS LSEIELPCSE
1460 1470 1480 1490 1500
DLNLETLSQA HVYIIAGACL SLGFRFAGSE NLSAFSCLHK FAKDFMNYLS
1510 1520 1530 1540 1550
APNASVTGPY NLETCLSVVL LSLAMVMAGS GNLKVLQLCR FLHMKTGGEM
1560 1570 1580 1590 1600
NYGFHLAHHM ALGLLFLGGG RYSLSTSNSS IAALLCALYP HFPAHSTDNR
1610 1620 1630 1640 1650
YHLQALRHLY VLAAEPRLLV PVDVDTNTPC YALIEVTYKG TQWYEQTKEE
1660 1670 1680 1690 1700
LMAPTLLPEL HLLKQMKVKG PRYWELLIDL SKGEQHLRSI LSKDGVLYVK
1710 1720 1730 1740 1750
LRAGQLSYKE DPMGWQSLLA QTVANRNSEA RAFKPETISS FTSDPALLSF
1760 1770 1780 1790 1800
AEYFCKPTVS MGPKQEILDL FSSILYECVA QETPEMLPAY IAMDQALRSL
1810 1820 1830 1840 1850
KKRDMSDTSD LWQIKLILEF FSSRSHQDRQ HTYPKRGLFI NSEFLPVVKC
1860 1870 1880 1890 1900
TVDATLDQWL QAGGDVCVHA YLSGQPVEKS QLNMLACFLV YHSVPAPRHL
1910 1920 1930 1940
PPMGLEGSTS FAELLYRFRH LKMPVRALLR LAPVLLGNPQ PMVM
Length:1,944
Mass (Da):215,994
Last modified:July 27, 2011 - v2
Checksum:i596DD3FC07CDA321
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti112 – 1121A → Q in CAA56450. (PubMed:7929068)Curated
Sequence conflicti348 – 3492AA → GV in CAA56450. (PubMed:7929068)Curated
Sequence conflicti643 – 6431N → K in BAC34976. (PubMed:16141072)Curated
Sequence conflicti1036 – 10361D → H in CAA56450. (PubMed:7929068)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X80169 mRNA. Translation: CAA56450.1.
AL928910 Genomic DNA. Translation: CAM25161.1.
CH466519 Genomic DNA. Translation: EDL28216.1.
BC139002 mRNA. Translation: AAI39003.1.
AK052404 mRNA. Translation: BAC34976.1.
AK077847 mRNA. Translation: BAC37032.2.
CCDSiCCDS16715.1.
PIRiA55117.
RefSeqiNP_032595.2. NM_008569.2.
UniGeneiMm.277408.
Mm.449185.

Genome annotation databases

EnsembliENSMUST00000014499; ENSMUSP00000014499; ENSMUSG00000014355.
GeneIDi17222.
KEGGimmu:17222.
UCSCiuc008mgq.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X80169 mRNA. Translation: CAA56450.1 .
AL928910 Genomic DNA. Translation: CAM25161.1 .
CH466519 Genomic DNA. Translation: EDL28216.1 .
BC139002 mRNA. Translation: AAI39003.1 .
AK052404 mRNA. Translation: BAC34976.1 .
AK077847 mRNA. Translation: BAC37032.2 .
CCDSi CCDS16715.1.
PIRi A55117.
RefSeqi NP_032595.2. NM_008569.2.
UniGenei Mm.277408.
Mm.449185.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 201352. 14 interactions.
IntActi P53995. 1 interaction.
MINTi MINT-4087721.
STRINGi 10090.ENSMUSP00000014499.

PTM databases

PhosphoSitei P53995.

Proteomic databases

MaxQBi P53995.
PaxDbi P53995.
PRIDEi P53995.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000014499 ; ENSMUSP00000014499 ; ENSMUSG00000014355 .
GeneIDi 17222.
KEGGi mmu:17222.
UCSCi uc008mgq.2. mouse.

Organism-specific databases

CTDi 64682.
MGIi MGI:103097. Anapc1.

Phylogenomic databases

eggNOGi NOG316940.
GeneTreei ENSGT00390000016757.
HOGENOMi HOG000231934.
HOVERGENi HBG045326.
InParanoidi P53995.
KOi K03348.
OMAi LAWTRNF.
OrthoDBi EOG77WWCQ.
TreeFami TF105441.

Enzyme and pathway databases

UniPathwayi UPA00143 .
Reactomei REACT_199115. Antigen processing: Ubiquitination & Proteasome degradation.
REACT_205250. Phosphorylation of the APC/C.
REACT_206033. Senescence-Associated Secretory Phenotype (SASP).
REACT_207679. Separation of Sister Chromatids.
REACT_210462. APC/C:Cdc20 mediated degradation of Cyclin B.
REACT_215601. Conversion from APC/C:Cdc20 to APC/C:Cdh1 in late anaphase.
REACT_219897. APC/C:Cdc20 mediated degradation of Securin.
REACT_222875. APC/C:Cdc20 mediated degradation of mitotic proteins.
REACT_225686. Autodegradation of Cdh1 by Cdh1:APC/C.
REACT_226135. APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1.

Miscellaneous databases

NextBioi 291630.
PROi P53995.
SOURCEi Search...

Gene expression databases

Bgeei P53995.
CleanExi MM_ANAPC1.
ExpressionAtlasi P53995. baseline and differential.
Genevestigatori P53995.

Family and domain databases

InterProi IPR024990. Apc1.
IPR002015. Proteasome/cyclosome_rpt.
[Graphical view ]
PANTHERi PTHR12827. PTHR12827. 1 hit.
Pfami PF12859. Apc1. 1 hit.
PF01851. PC_rep. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "A novel murine gene encoding a 216-kDa protein is related to a mitotic checkpoint regulator previously identified in Aspergillus nidulans."
    Starborg M., Brundell E., Gell K., Hoeoeg C.
    J. Biol. Chem. 269:24133-24137(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: CBA.
    Tissue: Testis.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  5. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-1063.
    Strain: C57BL/6J.
    Tissue: Embryonic lung and Forelimb.

Entry informationi

Entry nameiAPC1_MOUSE
AccessioniPrimary (citable) accession number: P53995
Secondary accession number(s): A2ATQ4, Q8BP33, Q8C772
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: July 27, 2011
Last modified: October 29, 2014
This is version 103 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3