P53995 (APC1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Anaphase-promoting complex subunit 1
Short name=APC1

Alternative name(s):
Cyclosome subunit 1
Mitotic checkpoint regulator
Testis-specific gene 24 protein

Gene names

Name:	Anapc1
Synonyms:	Mcpr, Tsg24

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	1944 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains By similarity.
Pathway	Protein modification; protein ubiquitination.
Subunit structure	The APC/C is composed of at least 12 subunits By similarity.
Tissue specificity	Abundantly expressed in proliferating fibroblasts, juvenile testis, adult brain and epididymis.
Developmental stage	Uniformly expressed throughout interphase of the cell cycle.
Post-translational modification	Phosphorylated. Phosphorylation on Ser-355 occurs specifically during mitosis By similarity.
Sequence similarities	Belongs to the APC1 family. Contains 4 PC repeats.

Ontologies

Keywords
Biological process	Cell cycle Cell division Mitosis Ubl conjugation pathway
Domain	Repeat
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell division Inferred from electronic annotation. Source: UniProtKB-KW mitosis Inferred from electronic annotation. Source: UniProtKB-KW protein K11-linked ubiquitination Inferred from sequence or structural similarity. Source: UniProtKB
Cellular_component	anaphase-promoting complex Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1944	1944	Anaphase-promoting complex subunit 1		PRO_0000215872
Regions
	Repeat	1297 – 1325	29	PC 1
	Repeat	1366 – 1404	39	PC 2
	Repeat	1467 – 1501	35	PC 3
	Repeat	1520 – 1552	33	PC 4
Amino acid modifications
	Modified residue	51	1	Phosphoserine By similarity
	Modified residue	60	1	Phosphoserine By similarity
	Modified residue	291	1	Phosphothreonine By similarity
	Modified residue	341	1	Phosphoserine By similarity
	Modified residue	355	1	Phosphoserine By similarity
	Modified residue	362	1	Phosphoserine By similarity
	Modified residue	373	1	Phosphoserine By similarity
	Modified residue	377	1	Phosphoserine By similarity
	Modified residue	537	1	Phosphothreonine By similarity
	Modified residue	547	1	Phosphoserine By similarity
	Modified residue	555	1	Phosphoserine By similarity
	Modified residue	571	1	Phosphotyrosine By similarity
	Modified residue	686	1	Phosphoserine By similarity
	Modified residue	688	1	Phosphoserine Ref.6
Experimental info
	Sequence conflict	112	1	A → Q in CAA56450. Ref.1
	Sequence conflict	348 – 349	2	AA → GV in CAA56450. Ref.1
	Sequence conflict	643	1	N → K in BAC34976. Ref.5
	Sequence conflict	1036	1	D → H in CAA56450. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P53995 [UniParc]. Last modified July 27, 2011. Version 2. Checksum: 596DD3FC07CDA321	FASTA	1,944	215,994
	10 20 30 40 50 60 MSNFSEERAT MIAAGDLQEF VPFGRDHCKH HPNALNLQLR QLQPASELWS SDGAAGLVGS 70 80 90 100 110 120 LQEVTIHEKQ KESWQLRKGV SEIGDAADYD EELYVAGNMV IWSKGSKSQA LAVYKAFTVD 130 140 150 160 170 180 STVQQALWCD FIISQDKSEK IYKSHELEKC ICILQSSCMN MHSIDGKDYI ASLPFQVANV 190 200 210 220 230 240 WATKYGLLFE RCSSSHEVPP SLPREPLPTM FSMLHPLDEI TPLVCKSGSL FGSSRVQYVV 250 260 270 280 290 300 DPAVKIVFLN IDPSIVMTYD AVQNVHSVWT LRRVKPEEEN AVLKFPEQAG TLQNATTSSS 310 320 330 340 350 360 LTAHLRSLSK GESPVASPFQ NYSSIHSQSR STSSPSLHSR SPSISNMAAL SRAHSPALGV 370 380 390 400 410 420 HSFSGAQRFN LSSHSQSPKR HSISHSPSGS FNDSFLAPET EPIVPELCID HLWTETLPNI 430 440 450 460 470 480 REKNSQASKV FITTDLCGQK FLCFLVEAQL QLRCVKFQES NDKTQLIFGS VTNIHAKDAA 490 500 510 520 530 540 PVEKIHTMLV LEGNGNLVLY TGVVRVGKVF IPGLPAPSLT MSNMMPRPST PLDGVGTPKP 550 560 570 580 590 600 LSKLLGSMDE VVLLSPVPEL RDSSKLNDSL YNEDCTFQQL GTYIHSVRDP VHNRVTLELS 610 620 630 640 650 660 NGSMVRITIP EVATSELVQT CLQAIKFILP KEVAIQVLVK WYNVHSAPGG PSCHSEWSLF 670 680 690 700 710 720 VICLLNMMGY NTDRLAWTRS FDFEGSLSPV IAPKKARPSD TGSDEDWEYL LNSEYHRNVE 730 740 750 760 770 780 SHLLNKSLCL TALEVSNAKD EDFSQNLSLD SSTLLFAHIP AIFFVLHLVY EELKLNTLMG 790 800 810 820 830 840 EGICSLIDLL VQLARDLKLD SYLDHYYRDS PTLVKTTGQV CTIDQGQMGF MHHPPFFTSE 850 860 870 880 890 900 PPSIYQWVSS CLKGEGMPPY PYLPGICERS RLVVLSIALY TLGDESCVSD ETCQYLSKVT 910 920 930 940 950 960 STPQKPQAEQ EENRFTFRHS ASVSVLAERL VVWMASVGFT LRDLETLPFG IALPIRDAIY 970 980 990 1000 1010 1020 HCREQPDSDW SEAVCLLIGR QDLSKQACEG NLPRGKSVLS SEVSSGTEAE EEDDGMNDLN 1030 1040 1050 1060 1070 1080 HEVMSLIWSE DLRVQDVRRL LQSAQPVRVN VVQYPELSDH EFIEEKENRL LQLCQRTMAL 1090 1100 1110 1120 1130 1140 PVGRGMFTLF SYHPVPTEPL PVPKLNLTGR APPRNTTVDL NSGNIDVPPN MASWASFHNG 1150 1160 1170 1180 1190 1200 VAAGLKIAPA SQIDSAWIVY NKPKHAELAN EYAGFLMALG LNGHLTKLAT LNIHDYLTKG 1210 1220 1230 1240 1250 1260 HEMTSIGLLL GVSAAKLGTM DMSITRLLSI HVPALLPPTS TELDVPHNVQ VAAVVGIGLV 1270 1280 1290 1300 1310 1320 YQGTAHRHTA EVLLAEIGRP PGPEMEYCTD RESYSLAAGL ALGMVCLGHG SNLIGMSDLN 1330 1340 1350 1360 1370 1380 VPEQLYQYMV GGHRRFQTGM HREKHKSPSY QIKEGDTINV DVTCPGATLA LAMIYLKTNN 1390 1400 1410 1420 1430 1440 RSIADWLRAP DTMYLLDFVK PEFLLLRTLA RCLILWDDIL PNSKWVDSNV PQIIRENSIS 1450 1460 1470 1480 1490 1500 LSEIELPCSE DLNLETLSQA HVYIIAGACL SLGFRFAGSE NLSAFSCLHK FAKDFMNYLS 1510 1520 1530 1540 1550 1560 APNASVTGPY NLETCLSVVL LSLAMVMAGS GNLKVLQLCR FLHMKTGGEM NYGFHLAHHM 1570 1580 1590 1600 1610 1620 ALGLLFLGGG RYSLSTSNSS IAALLCALYP HFPAHSTDNR YHLQALRHLY VLAAEPRLLV 1630 1640 1650 1660 1670 1680 PVDVDTNTPC YALIEVTYKG TQWYEQTKEE LMAPTLLPEL HLLKQMKVKG PRYWELLIDL 1690 1700 1710 1720 1730 1740 SKGEQHLRSI LSKDGVLYVK LRAGQLSYKE DPMGWQSLLA QTVANRNSEA RAFKPETISS 1750 1760 1770 1780 1790 1800 FTSDPALLSF AEYFCKPTVS MGPKQEILDL FSSILYECVA QETPEMLPAY IAMDQALRSL 1810 1820 1830 1840 1850 1860 KKRDMSDTSD LWQIKLILEF FSSRSHQDRQ HTYPKRGLFI NSEFLPVVKC TVDATLDQWL 1870 1880 1890 1900 1910 1920 QAGGDVCVHA YLSGQPVEKS QLNMLACFLV YHSVPAPRHL PPMGLEGSTS FAELLYRFRH 1930 1940 LKMPVRALLR LAPVLLGNPQ PMVM

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A novel murine gene encoding a 216-kDa protein is related to a mitotic checkpoint regulator previously identified in Aspergillus nidulans." Starborg M., Brundell E., Gell K., Hoeoeg C. J. Biol. Chem. 269:24133-24137(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: CBA. Tissue: Testis.
[2]	"Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P. PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C57BL/6J.
[3]	Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[5]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-1063. Strain: C57BL/6J. Tissue: Embryonic lung and Forelimb.
[6]	"Large scale localization of protein phosphorylation by use of electron capture dissociation mass spectrometry." Sweet S.M., Bailey C.M., Cunningham D.L., Heath J.K., Cooper H.J. Mol. Cell. Proteomics 8:904-912(2009) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-688, MASS SPECTROMETRY. Tissue: Embryonic fibroblast.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X80169 mRNA. Translation: CAA56450.1. AL928910 Genomic DNA. Translation: CAM25161.1. CH466519 Genomic DNA. Translation: EDL28216.1. BC139002 mRNA. Translation: AAI39003.1. AK052404 mRNA. Translation: BAC34976.1. AK077847 mRNA. Translation: BAC37032.2.
IPI	IPI00137228.
PIR	A55117.
RefSeq	NP_032595.2. NM_008569.2.
UniGene	Mm.277408. Mm.449185.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	10090.ENSMUSP00000014499.
PTM databases
PhosphoSite	P53995.
Proteomic databases
PaxDb	P53995.
PRIDE	P53995.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000014499; ENSMUSP00000014499; ENSMUSG00000014355.
GeneID	17222.
KEGG	mmu:17222.
Organism-specific databases
CTD	64682.
MGI	MGI:103097. Anapc1.
Phylogenomic databases
eggNOG	NOG316940.
GeneTree	ENSGT00390000016757.
HOGENOM	HOG000231934.
HOVERGEN	HBG045326.
InParanoid	A2ATQ4.
KO	K03348.
OMA	LAWTRNF.
OrthoDB	EOG4Z62MQ.
Enzyme and pathway databases
UniPathway	UPA00143.
Gene expression databases
ArrayExpress	P53995.
Bgee	P53995.
CleanEx	MM_ANAPC1.
Genevestigator	P53995.
GermOnline	ENSMUSG00000014355. Mus musculus.
Family and domain databases
InterPro	IPR024990. Apc1. [Graphical view]
PANTHER	PTHR12827. PTHR12827. 1 hit.
Pfam	PF12859. Apc1. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	291630.
SOURCE	Search...

Entry information

Entry name

APC1_MOUSE

Accession

Primary (citable) accession number: P53995
Secondary accession number(s): A2ATQ4, Q8BP33, Q8C772

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	July 27, 2011
Last modified:	May 1, 2013
This is version 89 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program