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Protein

IST1 homolog

Gene

IST1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

ESCRT-III-like protein involved in specific functions of the ESCRT machinery. Is required for efficient abscission during cytokinesis, but not for HIV-1 budding. The involvement in the MVB pathway is not established. Involved in recruiting VPS4A and/or VPS4B to the midbody of dividing cells (PubMed:19129479, PubMed:19129480). During late anaphase, involved in nuclear envelope reassembly and mitotic spindle disassembly together with the ESCRT-III complex: IST1 acts by mediating the recruitment of SPAST to the nuclear membrane, leading to microtubule severing (PubMed:26040712). Regulates early endosomal tubulation together with the ESCRT-III complex by mediating the recruitment of SPAST (PubMed:23897888).4 Publications

GO - Molecular functioni

  • cadherin binding involved in cell-cell adhesion Source: BHF-UCL
  • MIT domain binding Source: UniProtKB
  • protein complex binding Source: UniProtKB
  • protein domain specific binding Source: UniProtKB

GO - Biological processi

  • abscission Source: UniProtKB
  • cell division Source: UniProtKB
  • cytokinesis Source: UniProtKB
  • ESCRT III complex disassembly Source: ParkinsonsUK-UCL
  • establishment of protein localization Source: UniProtKB
  • multivesicular body assembly Source: ParkinsonsUK-UCL
  • positive regulation of collateral sprouting Source: Ensembl
  • positive regulation of proteolysis Source: UniProtKB
  • protein localization Source: UniProtKB
  • protein transport Source: InterPro
  • viral capsid secondary envelopment Source: UniProtKB
  • viral release from host cell Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division

Enzyme and pathway databases

BioCyciZFISH:ENSG00000140821-MONOMER.
ReactomeiR-HSA-6798695. Neutrophil degranulation.

Names & Taxonomyi

Protein namesi
Recommended name:
IST1 homolog
Short name:
hIST1
Alternative name(s):
Putative MAPK-activating protein PM28
Gene namesi
Name:IST1
Synonyms:KIAA0174
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 16

Organism-specific databases

HGNCiHGNC:28977. IST1.

Subcellular locationi

GO - Cellular componenti

  • cell-cell adherens junction Source: BHF-UCL
  • centrosome Source: UniProtKB
  • cytoplasmic, membrane-bounded vesicle Source: UniProtKB-SubCell
  • cytosol Source: UniProtKB
  • endoplasmic reticulum-Golgi intermediate compartment Source: UniProtKB
  • extracellular exosome Source: UniProtKB
  • Flemming body Source: UniProtKB
  • midbody Source: UniProtKB
  • nuclear envelope Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoplasmic vesicle, Cytoskeleton, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi323L → D: Diminishes interaction with VPS4A. Greatly diminishes interaction with VPS4A; when associated with A-353. 1 Publication1
Mutagenesisi326L → D: Diminishes interaction with VPS4A. Greatly diminishes interaction with VPS4A and abolishes interaction with VTA1; when associated with A-353. Greatly diminishes interaction with VPS4A; when associated with A-360. 1 Publication1
Mutagenesisi353L → A: Diminishes interaction with VPS4A. Greatly diminishes interaction with VPS4A and abolishes interaction with VTA1; when associated with D-326. Greatly diminishes interaction with VPS4A; when associated with D-323. 1 Publication1
Mutagenesisi360 – 361LK → AA: Abolishes interaction with VTA1, MITD1 and USP8; diminishes interaction with VPS4A. 1 Publication2
Mutagenesisi360L → A: Diminishes interaction with VPS4A. Greatly diminishes interaction with VPS4A; when associated with D-326. 1 Publication1

Organism-specific databases

DisGeNETi9798.
OpenTargetsiENSG00000182149.
PharmGKBiPA142671633.

Polymorphism and mutation databases

DMDMi1723119.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000507271 – 364IST1 homologAdd BLAST364

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei4PhosphoserineCombined sources1
Modified residuei43PhosphotyrosineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiP53990.
MaxQBiP53990.
PaxDbiP53990.
PeptideAtlasiP53990.
PRIDEiP53990.

PTM databases

iPTMnetiP53990.
PhosphoSitePlusiP53990.
SwissPalmiP53990.

Miscellaneous databases

PMAP-CutDBP53990.

Expressioni

Gene expression databases

BgeeiENSG00000182149.
CleanExiHS_KIAA0174.
ExpressionAtlasiP53990. baseline and differential.
GenevisibleiP53990. HS.

Organism-specific databases

HPAiHPA041802.
HPA054532.

Interactioni

Subunit structurei

Interacts with CHMP1A, CHMP1B, VPS4A and VTA1. Interacts with SPAST, STAMBP, and USP8. May interact with VPS37B. May associate with the ESCRT-I complex. Interacts with MITD1, in competition with VSP4 (PubMed:23015756). Interacts with SPG20 (via MIT domain); leading to the recruitment of SPG20 to midbodies (PubMed:20719964). Interacts with SPAST (PubMed:23897888, PubMed:26040712).6 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
APEHP137984EBI-945994,EBI-723792
ATXN1P542532EBI-945994,EBI-930964
CAPN7Q7Z4793EBI-945994,EBI-10213454
CAPN7Q9Y6W36EBI-945994,EBI-1765641
UBQLN1Q9UMX03EBI-945994,EBI-741480

GO - Molecular functioni

  • cadherin binding involved in cell-cell adhesion Source: BHF-UCL
  • MIT domain binding Source: UniProtKB
  • protein complex binding Source: UniProtKB
  • protein domain specific binding Source: UniProtKB

Protein-protein interaction databases

BioGridi115141. 45 interactors.
DIPiDIP-42546N.
IntActiP53990. 28 interactors.
MINTiMINT-1461091.
STRINGi9606.ENSP00000330408.

Structurei

Secondary structure

1364
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi8 – 45Combined sources38
Helixi49 – 81Combined sources33
Helixi83 – 87Combined sources5
Helixi94 – 96Combined sources3
Helixi97 – 110Combined sources14
Turni111 – 113Combined sources3
Helixi115 – 128Combined sources14
Helixi130 – 137Combined sources8
Turni138 – 141Combined sources4
Helixi146 – 151Combined sources6
Helixi159 – 173Combined sources15
Helixi181 – 185Combined sources5
Helixi350 – 360Combined sources11

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3FRRX-ray1.80A1-189[»]
3FRSX-ray2.61A5-189[»]
3JC1electron microscopy4.00Aa/Ac/Ae/Ag/Ai/Ak/Am/Ao/Aq/As/Au/Aw/Ay/Ba/Bc/Be/Bg/Bi/Bk/Bm/Bo/Bq/Bs/Bu/Bw/By/Ca/Cc/Ce/Cg6-187[»]
4U7EX-ray1.60A341-364[»]
4U7IX-ray1.79B341-364[»]
4U7YX-ray2.50B341-364[»]
4WZXX-ray1.39E342-364[»]
ProteinModelPortaliP53990.
SMRiP53990.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP53990.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 168Interaction with CHMP1A and CHMP1BAdd BLAST168
Regioni64 – 279Interaction with VPS37B1 PublicationAdd BLAST216
Regioni190 – 364Interaction with VTA1Add BLAST175
Regioni348 – 364Interaction with VPS4A, VTA1, MITD1 STAMBP and USP81 PublicationAdd BLAST17

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi321 – 332Type-2 MIT-interacting motifAdd BLAST12
Motifi351 – 361MIT-interacting motifAdd BLAST11

Sequence similaritiesi

Belongs to the IST1 family.Curated

Phylogenomic databases

eggNOGiKOG2027. Eukaryota.
ENOG410YG52. LUCA.
GeneTreeiENSGT00390000007453.
HOGENOMiHOG000205346.
InParanoidiP53990.
KOiK19476.
OMAiDTYDNFV.
OrthoDBiEOG091G0CZX.
PhylomeDBiP53990.
TreeFamiTF314258.

Family and domain databases

InterProiIPR005061. Ist1.
[Graphical view]
PANTHERiPTHR12161. PTHR12161. 1 hit.
PfamiPF03398. Ist1. 1 hit.
[Graphical view]

Sequences (6)i

Sequence statusi: Complete.

This entry describes 6 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P53990-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MLGSGFKAER LRVNLRLVIN RLKLLEKKKT ELAQKARKEI ADYLAAGKDE
60 70 80 90 100
RARIRVEHII REDYLVEAME ILELYCDLLL ARFGLIQSMK ELDSGLAESV
110 120 130 140 150
STLIWAAPRL QSEVAELKIV ADQLCAKYSK EYGKLCRTNQ IGTVNDRLMH
160 170 180 190 200
KLSVEAPPKI LVERYLIEIA KNYNVPYEPD SVVMAEAPPG VETDLIDVGF
210 220 230 240 250
TDDVKKGGPG RGGSGGFTAP VGGPDGTVPM PMPMPMPSAN TPFSYPLPKG
260 270 280 290 300
PSDFNGLPMG TYQAFPNIHP PQIPATPPSY ESVDDINADK NISSAQIVGP
310 320 330 340 350
GPKPEASAKL PSRPADNYDN FVLPELPSVP DTLPTASAGA STSASEDIDF
360
DDLSRRFEEL KKKT
Length:364
Mass (Da):39,751
Last modified:October 1, 1996 - v1
Checksum:i0DD3C186A52A4380
GO
Isoform 2 (identifier: P53990-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     228-228: V → VPM
     252-282: Missing.

Note: No experimental confirmation available.
Show »
Length:335
Mass (Da):36,622
Checksum:i7D0714269380E835
GO
Isoform 5 (identifier: P53990-5) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-1: M → MVFKLKTKEEQHSM
     237-237: P → PMP

Show »
Length:379
Mass (Da):41,566
Checksum:i37E0FEF4BB93C054
GO
Isoform 4 (identifier: P53990-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     228-228: V → VPM

Show »
Length:366
Mass (Da):39,979
Checksum:iE25DE7D7A5693AF8
GO
Isoform 3 (identifier: P53990-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     228-228: V → VPM
     283-364: VDDINADKNI...RRFEELKKKT → MTLMLIRISL...LMIFPGGLKS

Note: No experimental confirmation available.
Show »
Length:360
Mass (Da):39,928
Checksum:i5D0DDB4113DAA2BC
GO
Isoform 6 (identifier: P53990-6) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-148: Missing.
     228-228: V → VPM

Note: No experimental confirmation available. Gene prediction based on EST data.
Show »
Length:218
Mass (Da):23,089
Checksum:iB0405956455B9777
GO

Sequence cautioni

The sequence BAA11491 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0551181 – 148Missing in isoform 6. CuratedAdd BLAST148
Alternative sequenceiVSP_0470751M → MVFKLKTKEEQHSM in isoform 5. 1 Publication1
Alternative sequenceiVSP_017118228V → VPM in isoform 2, isoform 3, isoform 4 and isoform 6. 2 Publications1
Alternative sequenceiVSP_047076237P → PMP in isoform 5. 1 Publication1
Alternative sequenceiVSP_017119252 – 282Missing in isoform 2. 1 PublicationAdd BLAST31
Alternative sequenceiVSP_017120283 – 364VDDIN…LKKKT → MTLMLIRISLLHRLLVLDPS QKPLQSFLPDLQITMTTLSY QSCHLCQTHYQLHLLVPAPQ HLKTLTLMIFPGGLKS in isoform 3. 1 PublicationAdd BLAST82

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB097052 mRNA. Translation: BAC77405.1.
D79996 mRNA. Translation: BAA11491.2. Different initiation.
AK057258 mRNA. Translation: BAG51894.1.
AK293022 mRNA. Translation: BAF85711.1.
AK293040 mRNA. Translation: BAF85729.1.
AC009127 Genomic DNA. No translation available.
AC010653 Genomic DNA. No translation available.
BC000116 mRNA. Translation: AAH00116.1.
BC000430 mRNA. Translation: AAH00430.1.
BC004359 mRNA. Translation: AAH04359.1.
BC103745 mRNA. Translation: AAI03746.1.
CCDSiCCDS10905.1. [P53990-3]
CCDS59271.1. [P53990-5]
CCDS59272.1. [P53990-4]
CCDS59273.1. [P53990-2]
CCDS59274.1. [P53990-6]
RefSeqiNP_001257904.1. NM_001270975.1. [P53990-4]
NP_001257905.1. NM_001270976.1. [P53990-5]
NP_001257906.1. NM_001270977.1. [P53990-2]
NP_001257907.1. NM_001270978.1. [P53990-6]
NP_055576.2. NM_014761.3. [P53990-3]
UniGeneiHs.232194.

Genome annotation databases

EnsembliENST00000329908; ENSP00000330408; ENSG00000182149. [P53990-3]
ENST00000378798; ENSP00000368075; ENSG00000182149. [P53990-2]
ENST00000378799; ENSP00000368076; ENSG00000182149. [P53990-4]
ENST00000535424; ENSP00000438399; ENSG00000182149. [P53990-5]
ENST00000538850; ENSP00000463711; ENSG00000182149. [P53990-6]
ENST00000541571; ENSP00000455860; ENSG00000182149. [P53990-4]
ENST00000544564; ENSP00000457844; ENSG00000182149. [P53990-4]
ENST00000606369; ENSP00000475853; ENSG00000182149. [P53990-6]
GeneIDi9798.
KEGGihsa:9798.
UCSCiuc002fbk.3. human. [P53990-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB097052 mRNA. Translation: BAC77405.1.
D79996 mRNA. Translation: BAA11491.2. Different initiation.
AK057258 mRNA. Translation: BAG51894.1.
AK293022 mRNA. Translation: BAF85711.1.
AK293040 mRNA. Translation: BAF85729.1.
AC009127 Genomic DNA. No translation available.
AC010653 Genomic DNA. No translation available.
BC000116 mRNA. Translation: AAH00116.1.
BC000430 mRNA. Translation: AAH00430.1.
BC004359 mRNA. Translation: AAH04359.1.
BC103745 mRNA. Translation: AAI03746.1.
CCDSiCCDS10905.1. [P53990-3]
CCDS59271.1. [P53990-5]
CCDS59272.1. [P53990-4]
CCDS59273.1. [P53990-2]
CCDS59274.1. [P53990-6]
RefSeqiNP_001257904.1. NM_001270975.1. [P53990-4]
NP_001257905.1. NM_001270976.1. [P53990-5]
NP_001257906.1. NM_001270977.1. [P53990-2]
NP_001257907.1. NM_001270978.1. [P53990-6]
NP_055576.2. NM_014761.3. [P53990-3]
UniGeneiHs.232194.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3FRRX-ray1.80A1-189[»]
3FRSX-ray2.61A5-189[»]
3JC1electron microscopy4.00Aa/Ac/Ae/Ag/Ai/Ak/Am/Ao/Aq/As/Au/Aw/Ay/Ba/Bc/Be/Bg/Bi/Bk/Bm/Bo/Bq/Bs/Bu/Bw/By/Ca/Cc/Ce/Cg6-187[»]
4U7EX-ray1.60A341-364[»]
4U7IX-ray1.79B341-364[»]
4U7YX-ray2.50B341-364[»]
4WZXX-ray1.39E342-364[»]
ProteinModelPortaliP53990.
SMRiP53990.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi115141. 45 interactors.
DIPiDIP-42546N.
IntActiP53990. 28 interactors.
MINTiMINT-1461091.
STRINGi9606.ENSP00000330408.

PTM databases

iPTMnetiP53990.
PhosphoSitePlusiP53990.
SwissPalmiP53990.

Polymorphism and mutation databases

DMDMi1723119.

Proteomic databases

EPDiP53990.
MaxQBiP53990.
PaxDbiP53990.
PeptideAtlasiP53990.
PRIDEiP53990.

Protocols and materials databases

DNASUi9798.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000329908; ENSP00000330408; ENSG00000182149. [P53990-3]
ENST00000378798; ENSP00000368075; ENSG00000182149. [P53990-2]
ENST00000378799; ENSP00000368076; ENSG00000182149. [P53990-4]
ENST00000535424; ENSP00000438399; ENSG00000182149. [P53990-5]
ENST00000538850; ENSP00000463711; ENSG00000182149. [P53990-6]
ENST00000541571; ENSP00000455860; ENSG00000182149. [P53990-4]
ENST00000544564; ENSP00000457844; ENSG00000182149. [P53990-4]
ENST00000606369; ENSP00000475853; ENSG00000182149. [P53990-6]
GeneIDi9798.
KEGGihsa:9798.
UCSCiuc002fbk.3. human. [P53990-1]

Organism-specific databases

CTDi9798.
DisGeNETi9798.
GeneCardsiIST1.
HGNCiHGNC:28977. IST1.
HPAiHPA041802.
HPA054532.
MIMi616434. gene.
neXtProtiNX_P53990.
OpenTargetsiENSG00000182149.
PharmGKBiPA142671633.
HUGEiSearch...
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2027. Eukaryota.
ENOG410YG52. LUCA.
GeneTreeiENSGT00390000007453.
HOGENOMiHOG000205346.
InParanoidiP53990.
KOiK19476.
OMAiDTYDNFV.
OrthoDBiEOG091G0CZX.
PhylomeDBiP53990.
TreeFamiTF314258.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000140821-MONOMER.
ReactomeiR-HSA-6798695. Neutrophil degranulation.

Miscellaneous databases

ChiTaRSiIST1. human.
EvolutionaryTraceiP53990.
GenomeRNAii9798.
PMAP-CutDBP53990.
PROiP53990.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000182149.
CleanExiHS_KIAA0174.
ExpressionAtlasiP53990. baseline and differential.
GenevisibleiP53990. HS.

Family and domain databases

InterProiIPR005061. Ist1.
[Graphical view]
PANTHERiPTHR12161. PTHR12161. 1 hit.
PfamiPF03398. Ist1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiIST1_HUMAN
AccessioniPrimary (citable) accession number: P53990
Secondary accession number(s): A8KAH5
, J3QLU7, Q3SYM4, Q9BQ81, Q9BWN2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: November 30, 2016
This is version 141 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 16
    Human chromosome 16: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.