Reviewed, UniProtKB/Swiss-Prot P53990 (IST1_HUMAN)

Names and origin

Protein names

Recommended name:
    IST1 homolog
      Short name=hIST1
Alternative name(s):
    Putative MAPK-activating protein PM28

Gene names

Name:	KIAA0174
Synonyms:	IST1

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	364 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Proposed to be involved in specific functions of the ESCRT machinery. Is required for efficient abscission during cytokinesis, but not for HIV-1 budding. The involvment in the MVB pathway is not established. Involved in recruiting VPS4A and/or VPS4B to the midbody of dividing cells. Ref.8 Ref.9
Subunit structure	Interacts with CHMP1A, CHMP1B, VPS4A and VTA1. Interacts with SPAST, STAMBP, USP8 and MITD1. May interact with VPS37B. May associate with the ESCRT-I complex. Ref.8 Ref.9
Subcellular location	Cytoplasmic vesicle. Note: Localizes to the midbody of dividing cells. Ref.8 Ref.9
Sequence similarities	Belongs to the IST1 family.

Ontologies

Keywords
Biological process	Cell cycle Cell division
Cellular component	Cytoplasmic vesicle
Coding sequence diversity	Alternative splicing
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	ER-Golgi intermediate compartment Inferred from direct assay. Source: UniProtKB cytoplasmic membrane-bounded vesicle Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
ATXN1	P54253	1	EBI-945994,EBI-930964

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: P53990-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: P53990-2)

The sequence of this isoform differs from the canonical sequence as follows:
     228-228: V → VPM
     252-282: Missing.

Note: No experimental confirmation available.

Isoform 4 (identifier: P53990-4)

The sequence of this isoform differs from the canonical sequence as follows:
     228-228: V → VPM

Isoform 3 (identifier: P53990-3)

The sequence of this isoform differs from the canonical sequence as follows:
     228-228: V → VPM
     283-364: VDDINADKNI...RRFEELKKKT → MTLMLIRISL...LMIFPGGLKS

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 364	364	IST1 homolog		PRO_0000050727
Regions
	Region	1 – 168	168	Interaction with CHMP1A and CHMP1B
	Region	64 – 279	216	Interaction with VPS37B
	Region	190 – 364	175	Interaction with VTA1
	Region	348 – 364	17	Interaction with VPS4A, VTA1, MITD1 STAMBP and USP8
	Motif	321 – 332	12	Type-2 MIT-interacting motif
	Motif	351 – 361	11	MIT-interacting motif
Amino acid modifications
	Modified residue	43	1	Phosphotyrosine Ref.5 Ref.6
Natural variations
	Alternative sequence	228	1	V → VPM in isoform 2, isoform 3 and isoform 4.		VSP_017118
	Alternative sequence	252 – 282	31	Missing in isoform 2.		VSP_017119
	Alternative sequence	283 – 364	82	VDDIN…LKKKT → MTLMLIRISLLHRLLVLDPS QKPLQSFLPDLQITMTTLSY QSCHLCQTHYQLHLLVPAPQ HLKTLTLMIFPGGLKS in isoform 3.		VSP_017120
Experimental info
	Mutagenesis	323	1	L → D: Diminishes interaction with VPS4A. Ref.8 Ref.9
	Mutagenesis	323	1	L → D: Greatly diminishes interaction with VPS4A; when associated with A-353. Ref.8 Ref.9
	Mutagenesis	326	1	L → D: Diminishes interaction with VPS4A. Ref.8 Ref.9
	Mutagenesis	326	1	L → D: Greatly diminishes interaction with VPS4A and abolishes interaction with VTA1; when associated with A-353. Ref.8 Ref.9
	Mutagenesis	326	1	L → D: Greatly diminishes interaction with VPS4A; when associated with A-360.
	Mutagenesis	353	1	L → A: Diminishes interaction with VPS4A. Ref.8 Ref.9
	Mutagenesis	353	1	L → A: Greatly diminishes interaction with VPS4A and abolishes interaction with VTA1; when associated with D-326. Ref.8 Ref.9
	Mutagenesis	353	1	L → A: Greatly diminishes interaction with VPS4A; when associated with D-323.
	Mutagenesis	360 – 361	2	LK → AA: Abolishes interaction with VTA1, MITD1 and USP8; diminishes interaction with VPS4A. Ref.8 Ref.9
	Mutagenesis	360	1	L → A: Diminishes interaction with VPS4A. Ref.8 Ref.9
	Mutagenesis	360	1	L → A: Greatly diminishes interaction with VPS4A; when associated with D-326. Ref.8 Ref.9

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 0DD3C186A52A4380	FASTA	364	39,751
	10 20 30 40 50 60 MLGSGFKAER LRVNLRLVIN RLKLLEKKKT ELAQKARKEI ADYLAAGKDE RARIRVEHII 70 80 90 100 110 120 REDYLVEAME ILELYCDLLL ARFGLIQSMK ELDSGLAESV STLIWAAPRL QSEVAELKIV 130 140 150 160 170 180 ADQLCAKYSK EYGKLCRTNQ IGTVNDRLMH KLSVEAPPKI LVERYLIEIA KNYNVPYEPD 190 200 210 220 230 240 SVVMAEAPPG VETDLIDVGF TDDVKKGGPG RGGSGGFTAP VGGPDGTVPM PMPMPMPSAN 250 260 270 280 290 300 TPFSYPLPKG PSDFNGLPMG TYQAFPNIHP PQIPATPPSY ESVDDINADK NISSAQIVGP 310 320 330 340 350 360 GPKPEASAKL PSRPADNYDN FVLPELPSVP DTLPTASAGA STSASEDIDF DDLSRRFEEL KKKT
	Isoform 2. Checksum: 7D0714269380E835	FASTA	335	36,622
	10 20 30 40 50 60 MLGSGFKAER LRVNLRLVIN RLKLLEKKKT ELAQKARKEI ADYLAAGKDE RARIRVEHII 70 80 90 100 110 120 REDYLVEAME ILELYCDLLL ARFGLIQSMK ELDSGLAESV STLIWAAPRL QSEVAELKIV 130 140 150 160 170 180 ADQLCAKYSK EYGKLCRTNQ IGTVNDRLMH KLSVEAPPKI LVERYLIEIA KNYNVPYEPD 190 200 210 220 230 240 SVVMAEAPPG VETDLIDVGF TDDVKKGGPG RGGSGGFTAP VGGPDGTVPM PMPMPMPMPS 250 260 270 280 290 300 ANTPFSYPLP KGPVDDINAD KNISSAQIVG PGPKPEASAK LPSRPADNYD NFVLPELPSV 310 320 330 PDTLPTASAG ASTSASEDID FDDLSRRFEE LKKKT
	Isoform 4. Checksum: E25DE7D7A5693AF8	FASTA	366	39,979
	10 20 30 40 50 60 MLGSGFKAER LRVNLRLVIN RLKLLEKKKT ELAQKARKEI ADYLAAGKDE RARIRVEHII 70 80 90 100 110 120 REDYLVEAME ILELYCDLLL ARFGLIQSMK ELDSGLAESV STLIWAAPRL QSEVAELKIV 130 140 150 160 170 180 ADQLCAKYSK EYGKLCRTNQ IGTVNDRLMH KLSVEAPPKI LVERYLIEIA KNYNVPYEPD 190 200 210 220 230 240 SVVMAEAPPG VETDLIDVGF TDDVKKGGPG RGGSGGFTAP VGGPDGTVPM PMPMPMPMPS 250 260 270 280 290 300 ANTPFSYPLP KGPSDFNGLP MGTYQAFPNI HPPQIPATPP SYESVDDINA DKNISSAQIV 310 320 330 340 350 360 GPGPKPEASA KLPSRPADNY DNFVLPELPS VPDTLPTASA GASTSASEDI DFDDLSRRFE ELKKKT
	Isoform 3. Checksum: 5D0DDB4113DAA2BC	FASTA	360	39,928
	10 20 30 40 50 60 MLGSGFKAER LRVNLRLVIN RLKLLEKKKT ELAQKARKEI ADYLAAGKDE RARIRVEHII 70 80 90 100 110 120 REDYLVEAME ILELYCDLLL ARFGLIQSMK ELDSGLAESV STLIWAAPRL QSEVAELKIV 130 140 150 160 170 180 ADQLCAKYSK EYGKLCRTNQ IGTVNDRLMH KLSVEAPPKI LVERYLIEIA KNYNVPYEPD 190 200 210 220 230 240 SVVMAEAPPG VETDLIDVGF TDDVKKGGPG RGGSGGFTAP VGGPDGTVPM PMPMPMPMPS 250 260 270 280 290 300 ANTPFSYPLP KGPSDFNGLP MGTYQAFPNI HPPQIPATPP SYESMTLMLI RISLLHRLLV 310 320 330 340 350 360 LDPSQKPLQS FLPDLQITMT TLSYQSCHLC QTHYQLHLLV PAPQHLKTLT LMIFPGGLKS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Prediction of the coding sequences of unidentified human genes. V. The coding sequences of 40 new genes (KIAA0161-KIAA0200) deduced by analysis of cDNA clones from human cell line KG-1." Nagase T., Seki N., Ishikawa K., Tanaka A., Nomura N. DNA Res. 3:17-24(1996) [PubMed: 8724849] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Bone marrow.
[2]	"Large-scale identification and characterization of human genes that activate NF-kappaB and MAPK signaling pathways." Matsuda A., Suzuki Y., Honda G., Muramatsu S., Matsuzaki O., Nagano Y., Doi T., Shimotohno K., Harada T., Nishida E., Hayashi H., Sugano S. Oncogene 22:3307-3318(2003) [PubMed: 12761501] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Lung fibroblast.
[3]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 4).
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2; 3 AND 4). Tissue: Eye and Lung.
[5]	"Immunoaffinity profiling of tyrosine phosphorylation in cancer cells." Rush J., Moritz A., Lee K.A., Guo A., Goss V.L., Spek E.J., Zhang H., Zha X.-M., Polakiewicz R.D., Comb M.J. Nat. Biotechnol. 23:94-101(2005) [PubMed: 15592455] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-43, MASS SPECTROMETRY.
[6]	"Global survey of phosphotyrosine signaling identifies oncogenic kinases in lung cancer." Rikova K., Guo A., Zeng Q., Possemato A., Yu J., Haack H., Nardone J., Lee K., Reeves C., Li Y., Hu Y., Tan Z., Stokes M., Sullivan L., Mitchell J., Wetzel R., Macneill J., Ren J.M. , Yuan J., Bakalarski C.E., Villen J., Kornhauser J.M., Smith B., Li D., Zhou X., Gygi S.P., Gu T.-L., Polakiewicz R.D., Rush J., Comb M.J. Cell 131:1190-1203(2007) [PubMed: 18083107] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-43, MASS SPECTROMETRY.
[7]	Colinge J., Superti-Furga G., Bennett K.L. Submitted (OCT-2008) to UniProtKB Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.
[8]	"Essential role of hIST1 in cytokinesis." Agromayor M., Carlton J.G., Phelan J.P., Matthews D.R., Carlin L.M., Ameer-Beg S., Bowers K., Martin-Serrano J. Mol. Biol. Cell 20:1374-1387(2009) [PubMed: 19129480] [Abstract] Cited for: FUNCTION IN CYTOKINESIS, SUBCELLULAR LOCATION, INTERACTION WITH CHMP1A; CHMP1B; VPS4A; VTA1; MITD1; STAMBP; SPAST AND USP8, MUTAGENESIS OF 360-LEU-LYS-361.
[9]	"Biochemical analyses of human IST1 and its function in cytokinesis." Bajorek M., Morita E., Skalicky J.J., Morham S.G., Babst M., Sundquist W.I. Mol. Biol. Cell 20:1360-1373(2009) [PubMed: 19129479] [Abstract] Cited for: FUNCTION IN CYTOKINESIS, SUBCELLULAR LOCATION, INTERACTION WITH VPS37B; VTA1; CHMP1A; CHMP1B; VPS4A AND VPS4B, INTERACTION WITH THE ESCRT-1 COMPLEX, MUTAGENESIS OF LEU-323; LEU-326; LEU-353 AND LEU-360.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

AB097052 mRNA. Translation: BAC77405.1.
D79996 mRNA. Translation: BAA11491.2. Different initiation.
AK293022 mRNA. Translation: BAF85711.1.
BC000116 mRNA. Translation: AAH00116.1.
BC000430 mRNA. Translation: AAH00430.1.
BC004359 mRNA. Translation: AAH04359.1.
BC103745 mRNA. Translation: AAI03746.1.

IPI

IPI00024660.
IPI00420088.
IPI00643007.
IPI00939709.

RefSeq

NP_055576.2.

UniGene

Hs.232194

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3FRR	X-ray	1.80	A	1-189	[»]
3FRS	X-ray	2.61	A	5-189	[»]

ModBase

Search...

Protein-protein interaction databases

IntAct

P53990. 2 interactions.

STRING

P53990.

PTM databases

PhosphoSite

P53990.

Proteomic databases

PRIDE

P53990.

Genome annotation databases

Ensembl

ENST00000329908; ENSP00000330408; ENSG00000182149; Homo sapiens. [Genome view]
ENST00000378798; ENSP00000368075; ENSG00000182149; Homo sapiens. [Genome view]
ENST00000378799; ENSP00000368076; ENSG00000182149; Homo sapiens. [Genome view]
ENST00000424485; ENSP00000398505; ENSG00000182149; Homo sapiens. [Genome view]
ENST00000439924; ENSP00000410081; ENSG00000182149; Homo sapiens. [Genome view]
ENST00000456820; ENSP00000414942; ENSG00000182149; Homo sapiens. [Genome view]

GeneID

9798.

KEGG

hsa:9798.

UCSC

uc002fbj.1. human.
uc002fbk.1. human.
uc002fbl.1. human.

Organism-specific databases

CTD

9798.

GeneCards

GC16P070486.

HGNC

HGNC:28977. KIAA0174.

PharmGKB

PA142671633.

HUGE

Search...

GenAtlas

Search...

Phylogenomic databases

HOVERGEN

P53990.

OMA

NTPFSYP.

Gene expression databases

ArrayExpress

P53990.

Bgee

P53990.

CleanEx

HS_KIAA0174.

Genevestigator

P53990.

GermOnline

ENSG00000182149. Homo sapiens.

Family and domain databases

InterPro

IPR005061. DUF292_euk.
[Graphical view]

Pfam

PF03398. DUF292. 1 hit.
[Graphical view]

ProtoNet

Search...

Other Resources

NextBio

36900.

PMAP-CutDB

P53990.

Entry information

Entry name

IST1_HUMAN

Accession

Primary (citable) accession number: P53990
Secondary accession number(s): Q3SYM4, Q9BQ81, Q9BWN2

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	November 3, 2009
This is version 72 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.