P53990 (IST1_HUMAN) Reviewed, UniProtKB/Swiss-Prot
Last modified
May 1, 2013.
Version 104.
History...
Names·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
Names·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: IST1 homolog Short name=hIST1 Alternative name(s): Putative MAPK-activating protein PM28 | ||||
| Gene names |
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| Organism | Homo sapiens (Human) [Reference proteome] | ||||
| Taxonomic identifier | 9606 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo![]() |
Protein attributes
| Sequence length | 364 AA. |
| Sequence status | Complete. |
| Protein existence | Evidence at protein level |
General annotation (Comments)
| Function | Proposed to be involved in specific functions of the ESCRT machinery. Is required for efficient abscission during cytokinesis, but not for HIV-1 budding. The involvement in the MVB pathway is not established. Involved in recruiting VPS4A and/or VPS4B to the midbody of dividing cells. Ref.5 Ref.6 |
| Subunit structure | Interacts with CHMP1A, CHMP1B, VPS4A and VTA1. Interacts with SPAST, STAMBP, and USP8. May interact with VPS37B. May associate with the ESCRT-I complex. Interacts with MITD1, in competition with VSP4. Ref.5 Ref.6 Ref.9 |
| Subcellular location | Cytoplasmic vesicle. Note: Localizes to the midbody of dividing cells. Ref.5 Ref.6 |
| Sequence similarities | Belongs to the IST1 family. |
| Sequence caution | The sequence BAA11491.2 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened. |
Ontologies
Binary interactions
With | Entry | #Exp. | IntAct | Notes |
|---|---|---|---|---|
| ATXN1 | P54253 | 2 | EBI-945994,EBI-930964 |
Alternative products
| This entry describes 4 isoforms produced by alternative splicing. [Align] [Select] | ||||||
| Isoform 1 (identifier: P53990-1) This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. | ||||||
| Isoform 2 (identifier: P53990-2) The sequence of this isoform differs from the canonical sequence as follows: 228-228: V → VPM 252-282: Missing. | ||||||
| Note: No experimental confirmation available. | ||||||
| Isoform 4 (identifier: P53990-4) The sequence of this isoform differs from the canonical sequence as follows: 228-228: V → VPM | ||||||
| Isoform 3 (identifier: P53990-3) The sequence of this isoform differs from the canonical sequence as follows: 228-228: V → VPM 283-364: VDDINADKNI...RRFEELKKKT → MTLMLIRISL...LMIFPGGLKS | ||||||
| Note: No experimental confirmation available. |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | |||||||||||||||||||||||||
Molecule processing | ||||||||||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 364 | 364 | IST1 homolog | PRO_0000050727 | ||||||||||||||||||||||||||
Regions | ||||||||||||||||||||||||||||||
| Region | 1 – 168 | 168 | Interaction with CHMP1A and CHMP1B | |||||||||||||||||||||||||||
| Region | 64 – 279 | 216 | Interaction with VPS37B | |||||||||||||||||||||||||||
| Region | 190 – 364 | 175 | Interaction with VTA1 | |||||||||||||||||||||||||||
| Region | 348 – 364 | 17 | Interaction with VPS4A, VTA1, MITD1 STAMBP and USP8 | |||||||||||||||||||||||||||
| Motif | 321 – 332 | 12 | Type-2 MIT-interacting motif | |||||||||||||||||||||||||||
| Motif | 351 – 361 | 11 | MIT-interacting motif | |||||||||||||||||||||||||||
Natural variations | ||||||||||||||||||||||||||||||
| Alternative sequence | 228 | 1 | V → VPM in isoform 2, isoform 3 and isoform 4. | VSP_017118 | ||||||||||||||||||||||||||
| Alternative sequence | 252 – 282 | 31 | Missing in isoform 2. | VSP_017119 | ||||||||||||||||||||||||||
| Alternative sequence | 283 – 364 | 82 | VDDIN…LKKKT → MTLMLIRISLLHRLLVLDPS QKPLQSFLPDLQITMTTLSY QSCHLCQTHYQLHLLVPAPQ HLKTLTLMIFPGGLKS in isoform 3. | VSP_017120 | ||||||||||||||||||||||||||
Experimental info | ||||||||||||||||||||||||||||||
| Mutagenesis | 323 | 1 | L → D: Diminishes interaction with VPS4A. Ref.5 Ref.6 | |||||||||||||||||||||||||||
| Mutagenesis | 323 | 1 | L → D: Greatly diminishes interaction with VPS4A; when associated with A-353. Ref.5 Ref.6 | |||||||||||||||||||||||||||
| Mutagenesis | 326 | 1 | L → D: Diminishes interaction with VPS4A. Ref.5 Ref.6 | |||||||||||||||||||||||||||
| Mutagenesis | 326 | 1 | L → D: Greatly diminishes interaction with VPS4A and abolishes interaction with VTA1; when associated with A-353. Ref.5 Ref.6 | |||||||||||||||||||||||||||
| Mutagenesis | 326 | 1 | L → D: Greatly diminishes interaction with VPS4A; when associated with A-360. | |||||||||||||||||||||||||||
| Mutagenesis | 353 | 1 | L → A: Diminishes interaction with VPS4A. Ref.5 Ref.6 | |||||||||||||||||||||||||||
| Mutagenesis | 353 | 1 | L → A: Greatly diminishes interaction with VPS4A and abolishes interaction with VTA1; when associated with D-326. Ref.5 Ref.6 | |||||||||||||||||||||||||||
| Mutagenesis | 353 | 1 | L → A: Greatly diminishes interaction with VPS4A; when associated with D-323. | |||||||||||||||||||||||||||
| Mutagenesis | 360 – 361 | 2 | LK → AA: Abolishes interaction with VTA1, MITD1 and USP8; diminishes interaction with VPS4A. Ref.5 Ref.6 | |||||||||||||||||||||||||||
| Mutagenesis | 360 | 1 | L → A: Diminishes interaction with VPS4A. Ref.5 Ref.6 | |||||||||||||||||||||||||||
| Mutagenesis | 360 | 1 | L → A: Greatly diminishes interaction with VPS4A; when associated with D-326. Ref.5 Ref.6 | |||||||||||||||||||||||||||
Secondary structure | ||||||||||||||||||||||||||||||
Helix Strand Turn | ||||||||||||||||||||||||||||||
| Helix | 8 – 45 | 38 | ||||||||||||||||||||||||||||
| Helix | 49 – 81 | 33 | ||||||||||||||||||||||||||||
| Helix | 83 – 87 | 5 | ||||||||||||||||||||||||||||
| Helix | 94 – 96 | 3 | ||||||||||||||||||||||||||||
| Helix | 97 – 110 | 14 | ||||||||||||||||||||||||||||
| Turn | 111 – 113 | 3 | ||||||||||||||||||||||||||||
| Helix | 115 – 128 | 14 | ||||||||||||||||||||||||||||
| Helix | 130 – 137 | 8 | ||||||||||||||||||||||||||||
| Turn | 138 – 141 | 4 | ||||||||||||||||||||||||||||
| Helix | 146 – 151 | 6 | ||||||||||||||||||||||||||||
| Helix | 159 – 173 | 15 | ||||||||||||||||||||||||||||
| Helix | 181 – 185 | 5 | ||||||||||||||||||||||||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Prediction of the coding sequences of unidentified human genes. V. The coding sequences of 40 new genes (KIAA0161-KIAA0200) deduced by analysis of cDNA clones from human cell line KG-1." Nagase T., Seki N., Ishikawa K., Tanaka A., Nomura N. DNA Res. 3:17-24(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Bone marrow. |
| [2] | "Large-scale identification and characterization of human genes that activate NF-kappaB and MAPK signaling pathways." Matsuda A., Suzuki Y., Honda G., Muramatsu S., Matsuzaki O., Nagano Y., Doi T., Shimotohno K., Harada T., Nishida E., Hayashi H., Sugano S. Oncogene 22:3307-3318(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Lung fibroblast. |
| [3] | "Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. Sugano S.Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 4). |
| [4] | "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2; 3 AND 4). Tissue: Eye and Lung. |
| [5] | "Essential role of hIST1 in cytokinesis." Agromayor M., Carlton J.G., Phelan J.P., Matthews D.R., Carlin L.M., Ameer-Beg S., Bowers K., Martin-Serrano J. Mol. Biol. Cell 20:1374-1387(2009) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION IN CYTOKINESIS, SUBCELLULAR LOCATION, INTERACTION WITH CHMP1A; CHMP1B; VPS4A; VTA1; MITD1; STAMBP; SPAST AND USP8, MUTAGENESIS OF 360-LEU-LYS-361. |
| [6] | "Biochemical analyses of human IST1 and its function in cytokinesis." Bajorek M., Morita E., Skalicky J.J., Morham S.G., Babst M., Sundquist W.I. Mol. Biol. Cell 20:1360-1373(2009) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION IN CYTOKINESIS, SUBCELLULAR LOCATION, INTERACTION WITH VPS37B; VTA1; CHMP1A; CHMP1B; VPS4A AND VPS4B, INTERACTION WITH THE ESCRT-1 COMPLEX, MUTAGENESIS OF LEU-323; LEU-326; LEU-353 AND LEU-360. |
| [7] | "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis." Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M. Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Tissue: Cervix carcinoma. |
| [8] | "Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. |
| [9] | "MITD1 is recruited to midbodies by ESCRT-III and participates in cytokinesis." Lee S., Chang J., Renvoise B., Tipirneni A., Yang S., Blackstone C. Mol. Biol. Cell 23:4347-4361(2012) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH MITD1. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| EMBL GenBank DDBJ | AB097052 mRNA. Translation: BAC77405.1. D79996 mRNA. Translation: BAA11491.2. Different initiation. AK293022 mRNA. Translation: BAF85711.1. BC000116 mRNA. Translation: AAH00116.1. BC000430 mRNA. Translation: AAH00430.1. BC004359 mRNA. Translation: AAH04359.1. BC103745 mRNA. Translation: AAI03746.1. | ||||||||||||||||||
| IPI | IPI00024660. IPI00420088. IPI00643007. IPI00939709. | ||||||||||||||||||
| RefSeq | NP_001257904.1. NM_001270975.1. NP_001257906.1. NM_001270977.1. NP_055576.2. NM_014761.3. | ||||||||||||||||||
| UniGene | Hs.232194. | ||||||||||||||||||
3D structure databases | |||||||||||||||||||
| PDBe RCSB PDB PDBj |
| ||||||||||||||||||
| ProteinModelPortal | P53990. | ||||||||||||||||||
| ModBase | Search... | ||||||||||||||||||
Protein-protein interaction databases | |||||||||||||||||||
| DIP | DIP-42546N. | ||||||||||||||||||
| IntAct | P53990. 4 interactions. | ||||||||||||||||||
| MINT | MINT-1461091. | ||||||||||||||||||
| STRING | 9606.ENSP00000330408. | ||||||||||||||||||
PTM databases | |||||||||||||||||||
| PhosphoSite | P53990. | ||||||||||||||||||
Polymorphism databases | |||||||||||||||||||
| DMDM | 1723119. | ||||||||||||||||||
Proteomic databases | |||||||||||||||||||
| PaxDb | P53990. | ||||||||||||||||||
| PRIDE | P53990. | ||||||||||||||||||
Protocols and materials databases | |||||||||||||||||||
| DNASU | 9798. | ||||||||||||||||||
| StructuralBiologyKnowledgebase | Search... | ||||||||||||||||||
Genome annotation databases | |||||||||||||||||||
| Ensembl | ENST00000329908; ENSP00000330408; ENSG00000182149. ENST00000378798; ENSP00000368075; ENSG00000182149. ENST00000378799; ENSP00000368076; ENSG00000182149. ENST00000541571; ENSP00000455860; ENSG00000182149. ENST00000544564; ENSP00000457844; ENSG00000182149. | ||||||||||||||||||
| GeneID | 9798. | ||||||||||||||||||
| KEGG | hsa:9798. | ||||||||||||||||||
| UCSC | uc002fbk.1. human. uc002fbl.1. human. uc002fbm.1. human. | ||||||||||||||||||
Organism-specific databases | |||||||||||||||||||
| CTD | 9798. | ||||||||||||||||||
| GeneCards | GC16P071930. | ||||||||||||||||||
| HGNC | HGNC:28977. IST1. | ||||||||||||||||||
| HPA | HPA041802. | ||||||||||||||||||
| neXtProt | NX_P53990. | ||||||||||||||||||
| PharmGKB | PA142671633. | ||||||||||||||||||
| HUGE | Search... | ||||||||||||||||||
| GenAtlas | Search... | ||||||||||||||||||
Phylogenomic databases | |||||||||||||||||||
| eggNOG | NOG273475. | ||||||||||||||||||
| HOGENOM | HOG000205346. | ||||||||||||||||||
| InParanoid | P53990. | ||||||||||||||||||
| OrthoDB | EOG4DNF4W. | ||||||||||||||||||
Gene expression databases | |||||||||||||||||||
| ArrayExpress | P53990. | ||||||||||||||||||
| Bgee | P53990. | ||||||||||||||||||
| CleanEx | HS_KIAA0174. | ||||||||||||||||||
| Genevestigator | P53990. | ||||||||||||||||||
| GermOnline | ENSG00000182149. Homo sapiens. | ||||||||||||||||||
Family and domain databases | |||||||||||||||||||
| InterPro | IPR005061. DUF292_euk. [Graphical view] | ||||||||||||||||||
| Pfam | PF03398. Ist1. 1 hit. [Graphical view] | ||||||||||||||||||
| ProtoNet | Search... | ||||||||||||||||||
Other | |||||||||||||||||||
| ChiTaRS | IST1. human. | ||||||||||||||||||
| EvolutionaryTrace | P53990. | ||||||||||||||||||
| GenomeRNAi | 9798. | ||||||||||||||||||
| NextBio | 36900. | ||||||||||||||||||
| PMAP-CutDB | P53990. | ||||||||||||||||||
Entry information
| Entry name | IST1_HUMAN | ||||||||
| Accession | Primary (citable) accession number: P53990 Secondary accession number(s): Q3SYM4, Q9BQ81, Q9BWN2 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Chordata Protein Annotation Program | ||||||||
| Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. | ||||||||
Relevant documents
| Human chromosome 16 Human chromosome 16: entries, gene names and cross-references to MIM |
| PDB cross-references Index of Protein Data Bank (PDB) cross-references |
| SIMILARITY comments Index of protein domains and families |

Clusters with
