Reviewed,
UniProtKB/Swiss-Prot P53990 (IST1_HUMAN)
Last modified
November 3, 2009.
Version 72.
History...
Clusters with 100%,
90%,
50% identity |
Documents (3) |
Third-party data |
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Names and origin
| Protein names | Recommended name: IST1 homolog Short name=hIST1 Alternative name(s): Putative MAPK-activating protein PM28 | ||||
| Gene names |
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| Organism | Homo sapiens (Human) [Complete proteome] | ||||
| Taxonomic identifier | 9606 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Protein attributes
| Sequence length | 364 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Proposed to be involved in specific functions of the ESCRT machinery. Is required for efficient abscission during cytokinesis, but not for HIV-1 budding. The involvment in the MVB pathway is not established. Involved in recruiting VPS4A and/or VPS4B to the midbody of dividing cells. Ref.8 Ref.9 |
| Subunit structure | Interacts with CHMP1A, CHMP1B, VPS4A and VTA1. Interacts with SPAST, STAMBP, USP8 and MITD1. May interact with VPS37B. May associate with the ESCRT-I complex. Ref.8 Ref.9 |
| Subcellular location | Cytoplasmic vesicle. Note: Localizes to the midbody of dividing cells. Ref.8 Ref.9 |
| Sequence similarities | Belongs to the IST1 family. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Cell cycle Cell division |
| Cellular component | Cytoplasmic vesicle |
| Coding sequence diversity | Alternative splicing |
| PTM | Phosphoprotein |
| Technical term | 3D-structure Complete proteome |
| Gene Ontology (GO) | |
| Biological process | cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell divisionInferred from electronic annotation. Source: UniProtKB-KW |
| Cellular component | ER-Golgi intermediate compartment Inferred from direct assay. Source: UniProtKB cytoplasmic membrane-bounded vesicleInferred from electronic annotation. Source: UniProtKB-SubCell |
| Molecular function | protein binding Inferred from physical interaction. Source: IntAct |
| Complete GO annotation... | |
Alternative products
| This entry describes 4 isoforms produced by alternative splicing. [Align] [Select] | ||||||
| Isoform 1 (identifier: P53990-1) This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. | ||||||
| Isoform 2 (identifier: P53990-2) The sequence of this isoform differs from the canonical sequence as follows: 228-228: V → VPM 252-282: Missing. | ||||||
| Note: No experimental confirmation available. | ||||||
| Isoform 4 (identifier: P53990-4) The sequence of this isoform differs from the canonical sequence as follows: 228-228: V → VPM | ||||||
| Isoform 3 (identifier: P53990-3) The sequence of this isoform differs from the canonical sequence as follows: 228-228: V → VPM 283-364: VDDINADKNI...RRFEELKKKT → MTLMLIRISL...LMIFPGGLKS | ||||||
| Note: No experimental confirmation available. |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 364 | 364 | IST1 homolog | PRO_0000050727 | |||||
Regions | |||||||||
| Region | 1 – 168 | 168 | Interaction with CHMP1A and CHMP1B | ||||||
| Region | 64 – 279 | 216 | Interaction with VPS37B | ||||||
| Region | 190 – 364 | 175 | Interaction with VTA1 | ||||||
| Region | 348 – 364 | 17 | Interaction with VPS4A, VTA1, MITD1 STAMBP and USP8 | ||||||
| Motif | 321 – 332 | 12 | Type-2 MIT-interacting motif | ||||||
| Motif | 351 – 361 | 11 | MIT-interacting motif | ||||||
Amino acid modifications | |||||||||
| Modified residue | 43 | 1 | Phosphotyrosine Ref.5 Ref.6 | ||||||
Natural variations | |||||||||
| Alternative sequence | 228 | 1 | V → VPM in isoform 2, isoform 3 and isoform 4. | VSP_017118 | |||||
| Alternative sequence | 252 – 282 | 31 | Missing in isoform 2. | VSP_017119 | |||||
| Alternative sequence | 283 – 364 | 82 | VDDIN…LKKKT → MTLMLIRISLLHRLLVLDPS QKPLQSFLPDLQITMTTLSY QSCHLCQTHYQLHLLVPAPQ HLKTLTLMIFPGGLKS in isoform 3. | VSP_017120 | |||||
Experimental info | |||||||||
| Mutagenesis | 323 | 1 | L → D: Diminishes interaction with VPS4A. Ref.8 Ref.9 | ||||||
| Mutagenesis | 323 | 1 | L → D: Greatly diminishes interaction with VPS4A; when associated with A-353. Ref.8 Ref.9 | ||||||
| Mutagenesis | 326 | 1 | L → D: Diminishes interaction with VPS4A. Ref.8 Ref.9 | ||||||
| Mutagenesis | 326 | 1 | L → D: Greatly diminishes interaction with VPS4A and abolishes interaction with VTA1; when associated with A-353. Ref.8 Ref.9 | ||||||
| Mutagenesis | 326 | 1 | L → D: Greatly diminishes interaction with VPS4A; when associated with A-360. | ||||||
| Mutagenesis | 353 | 1 | L → A: Diminishes interaction with VPS4A. Ref.8 Ref.9 | ||||||
| Mutagenesis | 353 | 1 | L → A: Greatly diminishes interaction with VPS4A and abolishes interaction with VTA1; when associated with D-326. Ref.8 Ref.9 | ||||||
| Mutagenesis | 353 | 1 | L → A: Greatly diminishes interaction with VPS4A; when associated with D-323. | ||||||
| Mutagenesis | 360 – 361 | 2 | LK → AA: Abolishes interaction with VTA1, MITD1 and USP8; diminishes interaction with VPS4A. Ref.8 Ref.9 | ||||||
| Mutagenesis | 360 | 1 | L → A: Diminishes interaction with VPS4A. Ref.8 Ref.9 | ||||||
| Mutagenesis | 360 | 1 | L → A: Greatly diminishes interaction with VPS4A; when associated with D-326. Ref.8 Ref.9 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Prediction of the coding sequences of unidentified human genes. V. The coding sequences of 40 new genes (KIAA0161-KIAA0200) deduced by analysis of cDNA clones from human cell line KG-1." Nagase T., Seki N., Ishikawa K., Tanaka A., Nomura N. DNA Res. 3:17-24(1996) [PubMed: 8724849] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Bone marrow. |
| [2] | "Large-scale identification and characterization of human genes that activate NF-kappaB and MAPK signaling pathways." Matsuda A., Suzuki Y., Honda G., Muramatsu S., Matsuzaki O., Nagano Y., Doi T., Shimotohno K., Harada T., Nishida E., Hayashi H., Sugano S. Oncogene 22:3307-3318(2003) [PubMed: 12761501] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Lung fibroblast. |
| [3] | "Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. Sugano S.Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 4). |
| [4] | "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2; 3 AND 4). Tissue: Eye and Lung. |
| [5] | "Immunoaffinity profiling of tyrosine phosphorylation in cancer cells." Rush J., Moritz A., Lee K.A., Guo A., Goss V.L., Spek E.J., Zhang H., Zha X.-M., Polakiewicz R.D., Comb M.J. Nat. Biotechnol. 23:94-101(2005) [PubMed: 15592455] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-43, MASS SPECTROMETRY. |
| [6] | "Global survey of phosphotyrosine signaling identifies oncogenic kinases in lung cancer." Rikova K., Guo A., Zeng Q., Possemato A., Yu J., Haack H., Nardone J., Lee K., Reeves C., Li Y., Hu Y., Tan Z., Stokes M., Sullivan L., Mitchell J., Wetzel R., Macneill J., Ren J.M. Comb M.J.Cell 131:1190-1203(2007) [PubMed: 18083107] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-43, MASS SPECTROMETRY. |
| [7] | Colinge J., Superti-Furga G., Bennett K.L. Submitted (OCT-2008) to UniProtKB Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY. |
| [8] | "Essential role of hIST1 in cytokinesis." Agromayor M., Carlton J.G., Phelan J.P., Matthews D.R., Carlin L.M., Ameer-Beg S., Bowers K., Martin-Serrano J. Mol. Biol. Cell 20:1374-1387(2009) [PubMed: 19129480] [Abstract] Cited for: FUNCTION IN CYTOKINESIS, SUBCELLULAR LOCATION, INTERACTION WITH CHMP1A; CHMP1B; VPS4A; VTA1; MITD1; STAMBP; SPAST AND USP8, MUTAGENESIS OF 360-LEU-LYS-361. |
| [9] | "Biochemical analyses of human IST1 and its function in cytokinesis." Bajorek M., Morita E., Skalicky J.J., Morham S.G., Babst M., Sundquist W.I. Mol. Biol. Cell 20:1360-1373(2009) [PubMed: 19129479] [Abstract] Cited for: FUNCTION IN CYTOKINESIS, SUBCELLULAR LOCATION, INTERACTION WITH VPS37B; VTA1; CHMP1A; CHMP1B; VPS4A AND VPS4B, INTERACTION WITH THE ESCRT-1 COMPLEX, MUTAGENESIS OF LEU-323; LEU-326; LEU-353 AND LEU-360. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| AB097052 mRNA. Translation: BAC77405.1. D79996 mRNA. Translation: BAA11491.2. Different initiation. AK293022 mRNA. Translation: BAF85711.1. BC000116 mRNA. Translation: AAH00116.1. BC000430 mRNA. Translation: AAH00430.1. BC004359 mRNA. Translation: AAH04359.1. BC103745 mRNA. Translation: AAI03746.1. | |||||||||||||||||||
| IPI | IPI00024660. IPI00420088. IPI00643007. IPI00939709. | ||||||||||||||||||
| RefSeq | NP_055576.2. | ||||||||||||||||||
| UniGene | Hs.232194 | ||||||||||||||||||
3D structure databases | |||||||||||||||||||
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| ModBase | Search... | ||||||||||||||||||
Protein-protein interaction databases | |||||||||||||||||||
| IntAct | P53990. 2 interactions. | ||||||||||||||||||
| STRING | P53990. | ||||||||||||||||||
PTM databases | |||||||||||||||||||
| PhosphoSite | P53990. | ||||||||||||||||||
Proteomic databases | |||||||||||||||||||
| PRIDE | P53990. | ||||||||||||||||||
Genome annotation databases | |||||||||||||||||||
| Ensembl | ENST00000329908; ENSP00000330408; ENSG00000182149; Homo sapiens. [Genome view] ENST00000378798; ENSP00000368075; ENSG00000182149; Homo sapiens. [Genome view] ENST00000378799; ENSP00000368076; ENSG00000182149; Homo sapiens. [Genome view] ENST00000424485; ENSP00000398505; ENSG00000182149; Homo sapiens. [Genome view] ENST00000439924; ENSP00000410081; ENSG00000182149; Homo sapiens. [Genome view] ENST00000456820; ENSP00000414942; ENSG00000182149; Homo sapiens. [Genome view] | ||||||||||||||||||
| GeneID | 9798. | ||||||||||||||||||
| KEGG | hsa:9798. | ||||||||||||||||||
| UCSC | uc002fbj.1. human. uc002fbk.1. human. uc002fbl.1. human. | ||||||||||||||||||
Organism-specific databases | |||||||||||||||||||
| CTD | 9798. | ||||||||||||||||||
| GeneCards | GC16P070486. | ||||||||||||||||||
| HGNC | HGNC:28977. KIAA0174. | ||||||||||||||||||
| PharmGKB | PA142671633. | ||||||||||||||||||
| HUGE | Search... | ||||||||||||||||||
| GenAtlas | Search... | ||||||||||||||||||
Phylogenomic databases | |||||||||||||||||||
| HOVERGEN | P53990. | ||||||||||||||||||
| OMA | NTPFSYP. | ||||||||||||||||||
Gene expression databases | |||||||||||||||||||
| ArrayExpress | P53990. | ||||||||||||||||||
| Bgee | P53990. | ||||||||||||||||||
| CleanEx | HS_KIAA0174. | ||||||||||||||||||
| Genevestigator | P53990. | ||||||||||||||||||
| GermOnline | ENSG00000182149. Homo sapiens. | ||||||||||||||||||
Family and domain databases | |||||||||||||||||||
| InterPro | IPR005061. DUF292_euk. [Graphical view] | ||||||||||||||||||
| Pfam | PF03398. DUF292. 1 hit. [Graphical view] | ||||||||||||||||||
| ProtoNet | Search... | ||||||||||||||||||
Other Resources | |||||||||||||||||||
| NextBio | 36900. | ||||||||||||||||||
| PMAP-CutDB | P53990. | ||||||||||||||||||
Entry information
| Entry name | IST1_HUMAN | ||||||||
| Accession | Primary (citable) accession number: P53990 Secondary accession number(s): Q3SYM4, Q9BQ81, Q9BWN2 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||
| Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. | ||||||||
Relevant documents
| Human chromosome 16 Human chromosome 16: entries, gene names and cross-references to MIM |
| PDB cross-references Index of Protein Data Bank (PDB) cross-references |
| SIMILARITY comments Index of protein domains and families |

Clusters with


