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Protein

Monocarboxylate transporter 1

Gene

Slc16a1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Proton-coupled monocarboxylate transporter. Catalyzes the rapid transport across the plasma membrane of many monocarboxylates such as lactate, pyruvate, branched-chain oxo acids derived from leucine, valine and isoleucine, and the ketone bodies acetoacetate, beta-hydroxybutyrate and acetate. Depending on the tissue and on cicumstances, mediates the import or export of lactic acid and ketone bodies. Required for normal nutrient assimilation, increase of white adipose tissue and body weight gain when on a high-fat diet. Plays a role in cellular responses to a high-fat diet by modulating the cellular levels of lactate and pyruvate, small molecules that contribute to the regulation of central metabolic pathways and insulin secretion, with concomitant effects on plasma insulin levels and blood glucose homeostasis.5 Publications

Enzyme regulationi

Inhibited by stilbene disulfonates, such as di-isothiocyanostilbene disulfonate(DIDS), a cross-linking reagent that forms covalent linkages with lysine groups.1 Publication

GO - Molecular functioni

  • lactate transmembrane transporter activity Source: GO_Central
  • monocarboxylic acid transmembrane transporter activity Source: InterPro
  • organic cyclic compound binding Source: RGD
  • protein homodimerization activity Source: RGD
  • symporter activity Source: UniProtKB-KW

GO - Biological processi

  • behavioral response to nutrient Source: Ensembl
  • cellular response to organic cyclic compound Source: RGD
  • centrosome organization Source: Ensembl
  • glucose homeostasis Source: Ensembl
  • lactate transmembrane transport Source: RGD
  • lipid metabolic process Source: Ensembl
  • plasma membrane lactate transport Source: UniProtKB
  • regulation of insulin secretion Source: Ensembl
  • response to food Source: Ensembl
Complete GO annotation...

Keywords - Biological processi

Symport, Transport

Enzyme and pathway databases

ReactomeiR-RNO-210991. Basigin interactions.
R-RNO-433692. Proton-coupled monocarboxylate transport.
R-RNO-70268. Pyruvate metabolism.
SABIO-RKP53987.

Names & Taxonomyi

Protein namesi
Recommended name:
Monocarboxylate transporter 1
Short name:
MCT 1
Alternative name(s):
Solute carrier family 16 member 1
Gene namesi
Name:Slc16a1
Synonyms:Mct1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 2

Organism-specific databases

RGDi3690. Slc16a1.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 15CytoplasmicCuratedAdd BLAST15
Transmembranei16 – 36HelicalSequence analysisAdd BLAST21
Topological domaini37 – 59ExtracellularCuratedAdd BLAST23
Transmembranei60 – 80HelicalSequence analysisAdd BLAST21
Topological domaini81 – 86CytoplasmicSequence analysis6
Transmembranei87 – 107HelicalSequence analysisAdd BLAST21
Topological domaini108 – 111ExtracellularSequence analysis4
Transmembranei112 – 132HelicalSequence analysisAdd BLAST21
Topological domaini133 – 143CytoplasmicSequence analysisAdd BLAST11
Transmembranei144 – 164HelicalSequence analysisAdd BLAST21
Topological domaini165 – 166ExtracellularSequence analysis2
Transmembranei167 – 187HelicalSequence analysisAdd BLAST21
Topological domaini188 – 255CytoplasmicSequence analysisAdd BLAST68
Transmembranei256 – 276HelicalSequence analysisAdd BLAST21
Topological domaini277 – 291ExtracellularCuratedAdd BLAST15
Transmembranei292 – 312HelicalSequence analysisAdd BLAST21
Topological domaini313 – 321CytoplasmicSequence analysis9
Transmembranei322 – 342HelicalSequence analysisAdd BLAST21
Topological domaini343 – 346ExtracellularSequence analysis4
Transmembranei347 – 367HelicalSequence analysisAdd BLAST21
Topological domaini368 – 382CytoplasmicSequence analysisAdd BLAST15
Transmembranei383 – 403HelicalSequence analysisAdd BLAST21
Topological domaini404 – 415ExtracellularCuratedAdd BLAST12
Transmembranei416 – 436HelicalSequence analysisAdd BLAST21
Topological domaini437 – 494CytoplasmicCuratedAdd BLAST58

GO - Cellular componenti

  • centrosome Source: Ensembl
  • extracellular exosome Source: Ensembl
  • integral component of plasma membrane Source: UniProtKB
  • mitochondrion Source: Ensembl
  • plasma membrane Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi38K → Q or R: No effect on expression at the cell membrane, but abolishes lactate transport across the cell membrane. 1 Publication1
Mutagenesisi45K → Q or R: No effect on expression at the cell membrane. No effect on lactate transport across the cell membrane. 1 Publication1
Mutagenesisi86R → E or Q: No effect on expression at the cell membrane. No effect on lactate transport across the cell membrane. 1 Publication1
Mutagenesisi196R → E or Q: No effect on expression at the cell membrane. No effect on lactate transport across the cell membrane. 1 Publication1
Mutagenesisi282K → Q or R: No effect on expression at the cell membrane. No effect on lactate transport across the cell membrane. 1 Publication1
Mutagenesisi284K → Q or R: No effect on expression at the cell membrane. No effect on lactate transport across the cell membrane. 1 Publication1
Mutagenesisi290K → Q or R: No effect on expression at the cell membrane. No effect on lactate transport across the cell membrane. 1 Publication1
Mutagenesisi302D → R: Abolishes expression at the cell membrane. Abolishes lactate transport across the cell membrane without affecting expression at the cell membrane; when associated with E-306. 1 Publication1
Mutagenesisi306R → E: Abolishes expression at the cell membrane. Abolishes lactate transport across the cell membrane without affecting expression at the cell membrane; when associated with R-302. 1 Publication1
Mutagenesisi306R → K: No effect on expression at the cell membrane, but abolishes lactate transport across the cell membrane. 1 Publication1
Mutagenesisi413K → Q or R: No effect on expression at the cell membrane. No effect on lactate transport across the cell membrane. 1 Publication1

Chemistry databases

ChEMBLiCHEMBL2073709.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002113841 – 494Monocarboxylate transporter 1Add BLAST494

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei210PhosphoserineCombined sources1
Modified residuei213PhosphoserineCombined sources1
Modified residuei220PhosphoserineCombined sources1
Modified residuei224PhosphothreonineBy similarity1
Modified residuei230PhosphoserineCombined sources1
Modified residuei459PhosphothreonineBy similarity1
Modified residuei460PhosphoserineCombined sources1
Modified residuei461PhosphothreonineBy similarity1
Modified residuei476PhosphoserineBy similarity1
Modified residuei483PhosphoserineBy similarity1
Modified residuei484PhosphoserineBy similarity1
Modified residuei492PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP53987.
PRIDEiP53987.

PTM databases

iPTMnetiP53987.
PhosphoSitePlusiP53987.

Expressioni

Tissue specificityi

Detected in erythrocytes (at protein level). Detected in brain, heart, kidney, lung, muscle, jejunum enterocytes and brain capillaries.4 Publications

Gene expression databases

BgeeiENSRNOG00000019996.
GenevisibleiP53987. RN.

Interactioni

Subunit structurei

Interacts with BSG. Interacts with EMB. Interaction with either BSG or EMB is required for expression at the cell membrane.4 Publications

GO - Molecular functioni

  • protein homodimerization activity Source: RGD

Protein-protein interaction databases

BioGridi247107. 1 interactor.
STRINGi10116.ENSRNOP00000027234.

Chemistry databases

BindingDBiP53987.

Structurei

3D structure databases

ProteinModelPortaliP53987.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IMVV. Eukaryota.
ENOG41116NW. LUCA.
GeneTreeiENSGT00760000118926.
HOGENOMiHOG000280688.
HOVERGENiHBG006384.
InParanoidiP53987.
KOiK08179.
OMAiHMLAPLS.
OrthoDBiEOG091G05SW.
PhylomeDBiP53987.
TreeFamiTF313792.

Family and domain databases

CDDicd06174. MFS. 1 hit.
InterProiIPR004743. MCT.
IPR030757. MCT1.
IPR011701. MFS.
IPR020846. MFS_dom.
[Graphical view]
PANTHERiPTHR11360:SF24. PTHR11360:SF24. 1 hit.
PfamiPF07690. MFS_1. 1 hit.
[Graphical view]
SUPFAMiSSF103473. SSF103473. 2 hits.
TIGRFAMsiTIGR00892. 2A0113. 1 hit.
PROSITEiPS50850. MFS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P53987-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPPAIGGPVG YTPPDGGWGW AVVVGAFISI GFSYAFPKSI TVFFKEIEII
60 70 80 90 100
FSATTSEVSW ISSIMLAVMY AGGPISSILV NKYGSRPVMI AGGCLSGCGL
110 120 130 140 150
IAASFCNTVQ ELYFCIGVIG GLGLAFNLNP ALTMIGKYFY KKRPLANGLA
160 170 180 190 200
MAGSPVFLST LAPLNQAFFG IFGWRGSFLI LGGLLLNCCV AGSLMRPIGP
210 220 230 240 250
QQGKVEKLKS KESLQEAGKS DANTDLIGGS PKGEKLSVFQ TVNKFLDLSL
260 270 280 290 300
FTHRGFLLYL SGNVVMFFGL FTPLVFLSNY GKSKHFSSEK SAFLLSILAF
310 320 330 340 350
VDMVARPSMG LAANTRWIRP RVQYFFAASV VANGVCHLLA PLSTTYVGFC
360 370 380 390 400
IYAGVFGFAF GWLSSVLFET LMDLVGPQRF SSAVGLVTIV ECCPVLLGPP
410 420 430 440 450
LLGRLNDMYG DYKYTYWACG VILIIAGLYL FIGMGINYRL VAKEQKAEEK
460 470 480 490
KRDGKEDETS TDVDEKPKKT MKETQSPAPL QNSSGDPAEE ESPV
Length:494
Mass (Da):53,238
Last modified:October 1, 1996 - v1
Checksum:iCF82F8794CDBF057
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D63834 mRNA. Translation: BAA09894.1.
X86216 mRNA. Translation: CAA60116.1.
BC078877 mRNA. Translation: AAH78877.1.
AJ236865 mRNA. Translation: CAB37948.1.
PIRiJC4399.
RefSeqiNP_036848.1. NM_012716.2.
XP_017446125.1. XM_017590636.1.
UniGeneiRn.6085.

Genome annotation databases

EnsembliENSRNOT00000027234; ENSRNOP00000027234; ENSRNOG00000019996.
GeneIDi25027.
KEGGirno:25027.
UCSCiRGD:3690. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D63834 mRNA. Translation: BAA09894.1.
X86216 mRNA. Translation: CAA60116.1.
BC078877 mRNA. Translation: AAH78877.1.
AJ236865 mRNA. Translation: CAB37948.1.
PIRiJC4399.
RefSeqiNP_036848.1. NM_012716.2.
XP_017446125.1. XM_017590636.1.
UniGeneiRn.6085.

3D structure databases

ProteinModelPortaliP53987.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi247107. 1 interactor.
STRINGi10116.ENSRNOP00000027234.

Chemistry databases

BindingDBiP53987.
ChEMBLiCHEMBL2073709.

PTM databases

iPTMnetiP53987.
PhosphoSitePlusiP53987.

Proteomic databases

PaxDbiP53987.
PRIDEiP53987.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000027234; ENSRNOP00000027234; ENSRNOG00000019996.
GeneIDi25027.
KEGGirno:25027.
UCSCiRGD:3690. rat.

Organism-specific databases

CTDi6566.
RGDi3690. Slc16a1.

Phylogenomic databases

eggNOGiENOG410IMVV. Eukaryota.
ENOG41116NW. LUCA.
GeneTreeiENSGT00760000118926.
HOGENOMiHOG000280688.
HOVERGENiHBG006384.
InParanoidiP53987.
KOiK08179.
OMAiHMLAPLS.
OrthoDBiEOG091G05SW.
PhylomeDBiP53987.
TreeFamiTF313792.

Enzyme and pathway databases

ReactomeiR-RNO-210991. Basigin interactions.
R-RNO-433692. Proton-coupled monocarboxylate transport.
R-RNO-70268. Pyruvate metabolism.
SABIO-RKP53987.

Miscellaneous databases

PROiP53987.

Gene expression databases

BgeeiENSRNOG00000019996.
GenevisibleiP53987. RN.

Family and domain databases

CDDicd06174. MFS. 1 hit.
InterProiIPR004743. MCT.
IPR030757. MCT1.
IPR011701. MFS.
IPR020846. MFS_dom.
[Graphical view]
PANTHERiPTHR11360:SF24. PTHR11360:SF24. 1 hit.
PfamiPF07690. MFS_1. 1 hit.
[Graphical view]
SUPFAMiSSF103473. SSF103473. 2 hits.
TIGRFAMsiTIGR00892. 2A0113. 1 hit.
PROSITEiPS50850. MFS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMOT1_RAT
AccessioniPrimary (citable) accession number: P53987
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: November 30, 2016
This is version 134 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.