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P53987

- MOT1_RAT

UniProt

P53987 - MOT1_RAT

Protein

Monocarboxylate transporter 1

Gene

Slc16a1

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 113 (01 Oct 2014)
      Sequence version 1 (01 Oct 1996)
      Previous versions | rss
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    Functioni

    Proton-coupled monocarboxylate transporter. Catalyzes the rapid transport across the plasma membrane of many monocarboxylates such as lactate, pyruvate, branched-chain oxo acids derived from leucine, valine and isoleucine, and the ketone bodies acetoacetate, beta-hydroxybutyrate and acetate. Depending on the tissue and on cicumstances, mediates the import or export of lactic acid and ketone bodies. Required for normal nutrient assimilation, increase of white adipose tissue and body weight gain when on a high-fat diet. Plays a role in cellular responses to a high-fat diet by modulating the cellular levels of lactate and pyruvate, small molecules that contribute to the regulation of central metabolic pathways and insulin secretion, with concomitant effects on plasma insulin levels and blood glucose homeostasis.5 Publications

    Enzyme regulationi

    Inhibited by stilbene disulfonates, such as di-isothiocyanostilbene disulfonate(DIDS), a cross-linking reagent that forms covalent linkages with lysine groups.1 Publication

    GO - Molecular functioni

    1. organic cyclic compound binding Source: RGD
    2. protein binding Source: UniProtKB
    3. protein homodimerization activity Source: RGD
    4. secondary active monocarboxylate transmembrane transporter activity Source: InterPro
    5. symporter activity Source: UniProtKB-KW

    GO - Biological processi

    1. cellular response to organic cyclic compound Source: RGD
    2. lactate transmembrane transport Source: RGD
    3. plasma membrane lactate transport Source: UniProtKB

    Keywords - Biological processi

    Symport, Transport

    Enzyme and pathway databases

    ReactomeiREACT_203088. Pyruvate metabolism.
    SABIO-RKP53987.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Monocarboxylate transporter 1
    Short name:
    MCT 1
    Alternative name(s):
    Solute carrier family 16 member 1
    Gene namesi
    Name:Slc16a1
    Synonyms:Mct1
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 2

    Organism-specific databases

    RGDi3690. Slc16a1.

    Subcellular locationi

    Cell membrane 6 Publications; Multi-pass membrane protein 6 Publications

    GO - Cellular componenti

    1. integral component of plasma membrane Source: UniProtKB
    2. mitochondrion Source: Ensembl
    3. plasma membrane Source: RGD

    Keywords - Cellular componenti

    Cell membrane, Membrane

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi38 – 381K → Q or R: No effect on expression at the cell membrane, but abolishes lactate transport across the cell membrane. 1 Publication
    Mutagenesisi45 – 451K → Q or R: No effect on expression at the cell membrane. No effect on lactate transport across the cell membrane. 1 Publication
    Mutagenesisi86 – 861R → E or Q: No effect on expression at the cell membrane. No effect on lactate transport across the cell membrane. 1 Publication
    Mutagenesisi196 – 1961R → E or Q: No effect on expression at the cell membrane. No effect on lactate transport across the cell membrane. 1 Publication
    Mutagenesisi282 – 2821K → Q or R: No effect on expression at the cell membrane. No effect on lactate transport across the cell membrane. 1 Publication
    Mutagenesisi284 – 2841K → Q or R: No effect on expression at the cell membrane. No effect on lactate transport across the cell membrane. 1 Publication
    Mutagenesisi290 – 2901K → Q or R: No effect on expression at the cell membrane. No effect on lactate transport across the cell membrane. 1 Publication
    Mutagenesisi302 – 3021D → R: Abolishes expression at the cell membrane. Abolishes lactate transport across the cell membrane without affecting expression at the cell membrane; when associated with E-306. 1 Publication
    Mutagenesisi306 – 3061R → E: Abolishes expression at the cell membrane. Abolishes lactate transport across the cell membrane without affecting expression at the cell membrane; when associated with R-302. 1 Publication
    Mutagenesisi306 – 3061R → K: No effect on expression at the cell membrane, but abolishes lactate transport across the cell membrane. 1 Publication
    Mutagenesisi413 – 4131K → Q or R: No effect on expression at the cell membrane. No effect on lactate transport across the cell membrane. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 494494Monocarboxylate transporter 1PRO_0000211384Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei210 – 2101PhosphoserineBy similarity
    Modified residuei213 – 2131Phosphoserine1 Publication
    Modified residuei224 – 2241PhosphothreonineBy similarity
    Modified residuei230 – 2301PhosphoserineBy similarity
    Modified residuei459 – 4591PhosphothreonineBy similarity
    Modified residuei460 – 4601PhosphoserineBy similarity
    Modified residuei461 – 4611PhosphothreonineBy similarity
    Modified residuei476 – 4761PhosphoserineBy similarity
    Modified residuei492 – 4921PhosphoserineBy similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiP53987.
    PRIDEiP53987.

    PTM databases

    PhosphoSiteiP53987.

    Expressioni

    Tissue specificityi

    Detected in erythrocytes (at protein level). Detected in brain, heart, kidney, lung, muscle, jejunum enterocytes and brain capillaries.4 Publications

    Gene expression databases

    GenevestigatoriP53987.

    Interactioni

    Subunit structurei

    Interacts with BSG. Interacts with EMB. Interaction with either BSG or EMB is required for expression at the cell membrane.4 Publications

    Protein-protein interaction databases

    BioGridi247107. 1 interaction.
    STRINGi10116.ENSRNOP00000027234.

    Structurei

    3D structure databases

    ProteinModelPortaliP53987.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 1515CytoplasmicCuratedAdd
    BLAST
    Topological domaini37 – 5923ExtracellularCuratedAdd
    BLAST
    Topological domaini81 – 866CytoplasmicSequence Analysis
    Topological domaini108 – 1114ExtracellularSequence Analysis
    Topological domaini133 – 14311CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini165 – 1662ExtracellularSequence Analysis
    Topological domaini188 – 25568CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini277 – 29115ExtracellularCuratedAdd
    BLAST
    Topological domaini313 – 3219CytoplasmicSequence Analysis
    Topological domaini343 – 3464ExtracellularSequence Analysis
    Topological domaini368 – 38215CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini404 – 41512ExtracellularCuratedAdd
    BLAST
    Topological domaini437 – 49458CytoplasmicCuratedAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei16 – 3621HelicalSequence AnalysisAdd
    BLAST
    Transmembranei60 – 8021HelicalSequence AnalysisAdd
    BLAST
    Transmembranei87 – 10721HelicalSequence AnalysisAdd
    BLAST
    Transmembranei112 – 13221HelicalSequence AnalysisAdd
    BLAST
    Transmembranei144 – 16421HelicalSequence AnalysisAdd
    BLAST
    Transmembranei167 – 18721HelicalSequence AnalysisAdd
    BLAST
    Transmembranei256 – 27621HelicalSequence AnalysisAdd
    BLAST
    Transmembranei292 – 31221HelicalSequence AnalysisAdd
    BLAST
    Transmembranei322 – 34221HelicalSequence AnalysisAdd
    BLAST
    Transmembranei347 – 36721HelicalSequence AnalysisAdd
    BLAST
    Transmembranei383 – 40321HelicalSequence AnalysisAdd
    BLAST
    Transmembranei416 – 43621HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG0477.
    GeneTreeiENSGT00670000097675.
    HOGENOMiHOG000280688.
    HOVERGENiHBG006384.
    InParanoidiP53987.
    KOiK08179.
    OMAiQYFFAIS.
    OrthoDBiEOG7W9RTN.
    PhylomeDBiP53987.
    TreeFamiTF313792.

    Family and domain databases

    InterProiIPR011701. MFS.
    IPR020846. MFS_dom.
    IPR016196. MFS_dom_general_subst_transpt.
    IPR004743. Monocarb_transpt.
    [Graphical view]
    PfamiPF07690. MFS_1. 1 hit.
    [Graphical view]
    SUPFAMiSSF103473. SSF103473. 2 hits.
    TIGRFAMsiTIGR00892. 2A0113. 1 hit.
    PROSITEiPS50850. MFS. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P53987-1 [UniParc]FASTAAdd to Basket

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    MPPAIGGPVG YTPPDGGWGW AVVVGAFISI GFSYAFPKSI TVFFKEIEII    50
    FSATTSEVSW ISSIMLAVMY AGGPISSILV NKYGSRPVMI AGGCLSGCGL 100
    IAASFCNTVQ ELYFCIGVIG GLGLAFNLNP ALTMIGKYFY KKRPLANGLA 150
    MAGSPVFLST LAPLNQAFFG IFGWRGSFLI LGGLLLNCCV AGSLMRPIGP 200
    QQGKVEKLKS KESLQEAGKS DANTDLIGGS PKGEKLSVFQ TVNKFLDLSL 250
    FTHRGFLLYL SGNVVMFFGL FTPLVFLSNY GKSKHFSSEK SAFLLSILAF 300
    VDMVARPSMG LAANTRWIRP RVQYFFAASV VANGVCHLLA PLSTTYVGFC 350
    IYAGVFGFAF GWLSSVLFET LMDLVGPQRF SSAVGLVTIV ECCPVLLGPP 400
    LLGRLNDMYG DYKYTYWACG VILIIAGLYL FIGMGINYRL VAKEQKAEEK 450
    KRDGKEDETS TDVDEKPKKT MKETQSPAPL QNSSGDPAEE ESPV 494
    Length:494
    Mass (Da):53,238
    Last modified:October 1, 1996 - v1
    Checksum:iCF82F8794CDBF057
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D63834 mRNA. Translation: BAA09894.1.
    X86216 mRNA. Translation: CAA60116.1.
    BC078877 mRNA. Translation: AAH78877.1.
    AJ236865 mRNA. Translation: CAB37948.1.
    PIRiJC4399.
    RefSeqiNP_036848.1. NM_012716.2.
    UniGeneiRn.6085.

    Genome annotation databases

    EnsembliENSRNOT00000027234; ENSRNOP00000027234; ENSRNOG00000019996.
    GeneIDi25027.
    KEGGirno:25027.
    UCSCiRGD:3690. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D63834 mRNA. Translation: BAA09894.1 .
    X86216 mRNA. Translation: CAA60116.1 .
    BC078877 mRNA. Translation: AAH78877.1 .
    AJ236865 mRNA. Translation: CAB37948.1 .
    PIRi JC4399.
    RefSeqi NP_036848.1. NM_012716.2.
    UniGenei Rn.6085.

    3D structure databases

    ProteinModelPortali P53987.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 247107. 1 interaction.
    STRINGi 10116.ENSRNOP00000027234.

    Chemistry

    ChEMBLi CHEMBL2073709.

    PTM databases

    PhosphoSitei P53987.

    Proteomic databases

    PaxDbi P53987.
    PRIDEi P53987.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000027234 ; ENSRNOP00000027234 ; ENSRNOG00000019996 .
    GeneIDi 25027.
    KEGGi rno:25027.
    UCSCi RGD:3690. rat.

    Organism-specific databases

    CTDi 6566.
    RGDi 3690. Slc16a1.

    Phylogenomic databases

    eggNOGi COG0477.
    GeneTreei ENSGT00670000097675.
    HOGENOMi HOG000280688.
    HOVERGENi HBG006384.
    InParanoidi P53987.
    KOi K08179.
    OMAi QYFFAIS.
    OrthoDBi EOG7W9RTN.
    PhylomeDBi P53987.
    TreeFami TF313792.

    Enzyme and pathway databases

    Reactomei REACT_203088. Pyruvate metabolism.
    SABIO-RK P53987.

    Miscellaneous databases

    NextBioi 605163.
    PROi P53987.

    Gene expression databases

    Genevestigatori P53987.

    Family and domain databases

    InterProi IPR011701. MFS.
    IPR020846. MFS_dom.
    IPR016196. MFS_dom_general_subst_transpt.
    IPR004743. Monocarb_transpt.
    [Graphical view ]
    Pfami PF07690. MFS_1. 1 hit.
    [Graphical view ]
    SUPFAMi SSF103473. SSF103473. 2 hits.
    TIGRFAMsi TIGR00892. 2A0113. 1 hit.
    PROSITEi PS50850. MFS. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "cDNA cloning and functional characterization of rat intestinal monocarboxylate transporter."
      Takanaga H., Tamai I., Inaba S., Sai Y., Higashida H., Yamamoto H., Tsuji A.
      Biochem. Biophys. Res. Commun. 217:370-377(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, SUBCELLULAR LOCATION.
      Strain: Wistar.
      Tissue: Small intestine.
    2. "cDNA cloning of MCT1, a monocarboxylate transporter from rat skeletal muscle."
      Jackson V.N., Price N.T., Halestrap A.P.
      Biochim. Biophys. Acta 1238:193-196(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: Wistar.
      Tissue: Skeletal muscle.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Lung.
    4. "A monocarboxylate transporter MCT1 is located at the basolateral pole of rat jejunum."
      Orsenigo M.N., Tosco M., Bazzini C., Laforenza U., Faelli A.
      Exp. Physiol. 84:1033-1042(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 264-472, FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
      Tissue: Jejunal epithelium.
    5. "Interaction of the erythrocyte lactate transporter (monocarboxylate transporter 1) with an integral 70-kDa membrane glycoprotein of the immunoglobulin superfamily."
      Poole R.C., Halestrap A.P.
      J. Biol. Chem. 272:14624-14628(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH EMB, TISSUE SPECIFICITY, SUBCELLULAR LOCATION.
    6. "Fluorescence resonance energy transfer studies on the interaction between the lactate transporter MCT1 and CD147 provide information on the topology and stoichiometry of the complex in situ."
      Wilson M.C., Meredith D., Halestrap A.P.
      J. Biol. Chem. 277:3666-3672(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH BSG, FUNCTION, SUBCELLULAR LOCATION.
    7. "Phosphoproteomic analysis of rat liver by high capacity IMAC and LC-MS/MS."
      Moser K., White F.M.
      J. Proteome Res. 5:98-104(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-213, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    8. "The role of charged residues in the transmembrane helices of monocarboxylate transporter 1 and its ancillary protein basigin in determining plasma membrane expression and catalytic activity."
      Manoharan C., Wilson M.C., Sessions R.B., Halestrap A.P.
      Mol. Membr. Biol. 23:486-498(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH BSG, TOPOLOGY, 3D-STRUCTURE MODELING, MUTAGENESIS OF ARG-86; ARG-196; ASP-302 AND ARG-306.
    9. "Studies on the DIDS-binding site of monocarboxylate transporter 1 suggest a homology model of the open conformation and a plausible translocation cycle."
      Wilson M.C., Meredith D., Bunnun C., Sessions R.B., Halestrap A.P.
      J. Biol. Chem. 284:20011-20021(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH EMB, SUBCELLULAR LOCATION, TOPOLOGY, 3D-STRUCTURE MODELING, MUTAGENESIS OF LYS-38; LYS-45; LYS-282; LYS-284; LYS-290 AND LYS-413, ENZYME REGULATION, TISSUE SPECIFICITY.

    Entry informationi

    Entry nameiMOT1_RAT
    AccessioniPrimary (citable) accession number: P53987
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 113 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3