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P53987

- MOT1_RAT

UniProt

P53987 - MOT1_RAT

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Protein
Monocarboxylate transporter 1
Gene
Slc16a1, Mct1
Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Proton-coupled monocarboxylate transporter. Catalyzes the rapid transport across the plasma membrane of many monocarboxylates such as lactate, pyruvate, branched-chain oxo acids derived from leucine, valine and isoleucine, and the ketone bodies acetoacetate, beta-hydroxybutyrate and acetate. Depending on the tissue and on cicumstances, mediates the import or export of lactic acid and ketone bodies. Required for normal nutrient assimilation, increase of white adipose tissue and body weight gain when on a high-fat diet. Plays a role in cellular responses to a high-fat diet by modulating the cellular levels of lactate and pyruvate, small molecules that contribute to the regulation of central metabolic pathways and insulin secretion, with concomitant effects on plasma insulin levels and blood glucose homeostasis.5 Publications

Enzyme regulationi

Inhibited by stilbene disulfonates, such as di-isothiocyanostilbene disulfonate(DIDS), a cross-linking reagent that forms covalent linkages with lysine groups.1 Publication

GO - Molecular functioni

  1. organic cyclic compound binding Source: RGD
  2. protein binding Source: UniProtKB
  3. protein homodimerization activity Source: RGD
  4. secondary active monocarboxylate transmembrane transporter activity Source: InterPro
  5. symporter activity Source: UniProtKB-KW

GO - Biological processi

  1. cellular response to organic cyclic compound Source: RGD
  2. lactate transmembrane transport Source: RGD
  3. plasma membrane lactate transport Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Symport, Transport

Enzyme and pathway databases

ReactomeiREACT_203088. Pyruvate metabolism.
SABIO-RKP53987.

Names & Taxonomyi

Protein namesi
Recommended name:
Monocarboxylate transporter 1
Short name:
MCT 1
Alternative name(s):
Solute carrier family 16 member 1
Gene namesi
Name:Slc16a1
Synonyms:Mct1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 2

Organism-specific databases

RGDi3690. Slc16a1.

Subcellular locationi

Cell membrane; Multi-pass membrane protein 6 Publications

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 1515Cytoplasmic Inferred
Add
BLAST
Transmembranei16 – 3621Helical; Reviewed prediction
Add
BLAST
Topological domaini37 – 5923Extracellular Inferred
Add
BLAST
Transmembranei60 – 8021Helical; Reviewed prediction
Add
BLAST
Topological domaini81 – 866Cytoplasmic Reviewed prediction
Transmembranei87 – 10721Helical; Reviewed prediction
Add
BLAST
Topological domaini108 – 1114Extracellular Reviewed prediction
Transmembranei112 – 13221Helical; Reviewed prediction
Add
BLAST
Topological domaini133 – 14311Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei144 – 16421Helical; Reviewed prediction
Add
BLAST
Topological domaini165 – 1662Extracellular Reviewed prediction
Transmembranei167 – 18721Helical; Reviewed prediction
Add
BLAST
Topological domaini188 – 25568Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei256 – 27621Helical; Reviewed prediction
Add
BLAST
Topological domaini277 – 29115Extracellular Inferred
Add
BLAST
Transmembranei292 – 31221Helical; Reviewed prediction
Add
BLAST
Topological domaini313 – 3219Cytoplasmic Reviewed prediction
Transmembranei322 – 34221Helical; Reviewed prediction
Add
BLAST
Topological domaini343 – 3464Extracellular Reviewed prediction
Transmembranei347 – 36721Helical; Reviewed prediction
Add
BLAST
Topological domaini368 – 38215Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei383 – 40321Helical; Reviewed prediction
Add
BLAST
Topological domaini404 – 41512Extracellular Inferred
Add
BLAST
Transmembranei416 – 43621Helical; Reviewed prediction
Add
BLAST
Topological domaini437 – 49458Cytoplasmic Inferred
Add
BLAST

GO - Cellular componenti

  1. integral component of plasma membrane Source: UniProtKB
  2. mitochondrion Source: Ensembl
  3. plasma membrane Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi38 – 381K → Q or R: No effect on expression at the cell membrane, but abolishes lactate transport across the cell membrane. 1 Publication
Mutagenesisi45 – 451K → Q or R: No effect on expression at the cell membrane. No effect on lactate transport across the cell membrane. 1 Publication
Mutagenesisi86 – 861R → E or Q: No effect on expression at the cell membrane. No effect on lactate transport across the cell membrane. 1 Publication
Mutagenesisi196 – 1961R → E or Q: No effect on expression at the cell membrane. No effect on lactate transport across the cell membrane. 1 Publication
Mutagenesisi282 – 2821K → Q or R: No effect on expression at the cell membrane. No effect on lactate transport across the cell membrane. 1 Publication
Mutagenesisi284 – 2841K → Q or R: No effect on expression at the cell membrane. No effect on lactate transport across the cell membrane. 1 Publication
Mutagenesisi290 – 2901K → Q or R: No effect on expression at the cell membrane. No effect on lactate transport across the cell membrane. 1 Publication
Mutagenesisi302 – 3021D → R: Abolishes expression at the cell membrane. Abolishes lactate transport across the cell membrane without affecting expression at the cell membrane; when associated with E-306. 1 Publication
Mutagenesisi306 – 3061R → E: Abolishes expression at the cell membrane. Abolishes lactate transport across the cell membrane without affecting expression at the cell membrane; when associated with R-302. 1 Publication
Mutagenesisi306 – 3061R → K: No effect on expression at the cell membrane, but abolishes lactate transport across the cell membrane. 1 Publication
Mutagenesisi413 – 4131K → Q or R: No effect on expression at the cell membrane. No effect on lactate transport across the cell membrane. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 494494Monocarboxylate transporter 1
PRO_0000211384Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei210 – 2101Phosphoserine By similarity
Modified residuei213 – 2131Phosphoserine1 Publication
Modified residuei224 – 2241Phosphothreonine By similarity
Modified residuei230 – 2301Phosphoserine By similarity
Modified residuei459 – 4591Phosphothreonine By similarity
Modified residuei460 – 4601Phosphoserine By similarity
Modified residuei461 – 4611Phosphothreonine By similarity
Modified residuei476 – 4761Phosphoserine By similarity
Modified residuei492 – 4921Phosphoserine By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP53987.
PRIDEiP53987.

PTM databases

PhosphoSiteiP53987.

Expressioni

Tissue specificityi

Detected in erythrocytes (at protein level). Detected in brain, heart, kidney, lung, muscle, jejunum enterocytes and brain capillaries.4 Publications

Gene expression databases

GenevestigatoriP53987.

Interactioni

Subunit structurei

Interacts with BSG. Interacts with EMB. Interaction with either BSG or EMB is required for expression at the cell membrane.4 Publications

Protein-protein interaction databases

BioGridi247107. 1 interaction.
STRINGi10116.ENSRNOP00000027234.

Structurei

3D structure databases

ProteinModelPortaliP53987.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0477.
GeneTreeiENSGT00670000097675.
HOGENOMiHOG000280688.
HOVERGENiHBG006384.
InParanoidiP53987.
KOiK08179.
OMAiQYFFAIS.
OrthoDBiEOG7W9RTN.
PhylomeDBiP53987.
TreeFamiTF313792.

Family and domain databases

InterProiIPR011701. MFS.
IPR020846. MFS_dom.
IPR016196. MFS_dom_general_subst_transpt.
IPR004743. Monocarb_transpt.
[Graphical view]
PfamiPF07690. MFS_1. 1 hit.
[Graphical view]
SUPFAMiSSF103473. SSF103473. 2 hits.
TIGRFAMsiTIGR00892. 2A0113. 1 hit.
PROSITEiPS50850. MFS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P53987-1 [UniParc]FASTAAdd to Basket

« Hide

MPPAIGGPVG YTPPDGGWGW AVVVGAFISI GFSYAFPKSI TVFFKEIEII    50
FSATTSEVSW ISSIMLAVMY AGGPISSILV NKYGSRPVMI AGGCLSGCGL 100
IAASFCNTVQ ELYFCIGVIG GLGLAFNLNP ALTMIGKYFY KKRPLANGLA 150
MAGSPVFLST LAPLNQAFFG IFGWRGSFLI LGGLLLNCCV AGSLMRPIGP 200
QQGKVEKLKS KESLQEAGKS DANTDLIGGS PKGEKLSVFQ TVNKFLDLSL 250
FTHRGFLLYL SGNVVMFFGL FTPLVFLSNY GKSKHFSSEK SAFLLSILAF 300
VDMVARPSMG LAANTRWIRP RVQYFFAASV VANGVCHLLA PLSTTYVGFC 350
IYAGVFGFAF GWLSSVLFET LMDLVGPQRF SSAVGLVTIV ECCPVLLGPP 400
LLGRLNDMYG DYKYTYWACG VILIIAGLYL FIGMGINYRL VAKEQKAEEK 450
KRDGKEDETS TDVDEKPKKT MKETQSPAPL QNSSGDPAEE ESPV 494
Length:494
Mass (Da):53,238
Last modified:October 1, 1996 - v1
Checksum:iCF82F8794CDBF057
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D63834 mRNA. Translation: BAA09894.1.
X86216 mRNA. Translation: CAA60116.1.
BC078877 mRNA. Translation: AAH78877.1.
AJ236865 mRNA. Translation: CAB37948.1.
PIRiJC4399.
RefSeqiNP_036848.1. NM_012716.2.
UniGeneiRn.6085.

Genome annotation databases

EnsembliENSRNOT00000027234; ENSRNOP00000027234; ENSRNOG00000019996.
GeneIDi25027.
KEGGirno:25027.
UCSCiRGD:3690. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D63834 mRNA. Translation: BAA09894.1 .
X86216 mRNA. Translation: CAA60116.1 .
BC078877 mRNA. Translation: AAH78877.1 .
AJ236865 mRNA. Translation: CAB37948.1 .
PIRi JC4399.
RefSeqi NP_036848.1. NM_012716.2.
UniGenei Rn.6085.

3D structure databases

ProteinModelPortali P53987.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 247107. 1 interaction.
STRINGi 10116.ENSRNOP00000027234.

Chemistry

ChEMBLi CHEMBL2073709.

PTM databases

PhosphoSitei P53987.

Proteomic databases

PaxDbi P53987.
PRIDEi P53987.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSRNOT00000027234 ; ENSRNOP00000027234 ; ENSRNOG00000019996 .
GeneIDi 25027.
KEGGi rno:25027.
UCSCi RGD:3690. rat.

Organism-specific databases

CTDi 6566.
RGDi 3690. Slc16a1.

Phylogenomic databases

eggNOGi COG0477.
GeneTreei ENSGT00670000097675.
HOGENOMi HOG000280688.
HOVERGENi HBG006384.
InParanoidi P53987.
KOi K08179.
OMAi QYFFAIS.
OrthoDBi EOG7W9RTN.
PhylomeDBi P53987.
TreeFami TF313792.

Enzyme and pathway databases

Reactomei REACT_203088. Pyruvate metabolism.
SABIO-RK P53987.

Miscellaneous databases

NextBioi 605163.
PROi P53987.

Gene expression databases

Genevestigatori P53987.

Family and domain databases

InterProi IPR011701. MFS.
IPR020846. MFS_dom.
IPR016196. MFS_dom_general_subst_transpt.
IPR004743. Monocarb_transpt.
[Graphical view ]
Pfami PF07690. MFS_1. 1 hit.
[Graphical view ]
SUPFAMi SSF103473. SSF103473. 2 hits.
TIGRFAMsi TIGR00892. 2A0113. 1 hit.
PROSITEi PS50850. MFS. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "cDNA cloning and functional characterization of rat intestinal monocarboxylate transporter."
    Takanaga H., Tamai I., Inaba S., Sai Y., Higashida H., Yamamoto H., Tsuji A.
    Biochem. Biophys. Res. Commun. 217:370-377(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, SUBCELLULAR LOCATION.
    Strain: Wistar.
    Tissue: Small intestine.
  2. "cDNA cloning of MCT1, a monocarboxylate transporter from rat skeletal muscle."
    Jackson V.N., Price N.T., Halestrap A.P.
    Biochim. Biophys. Acta 1238:193-196(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Wistar.
    Tissue: Skeletal muscle.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Lung.
  4. "A monocarboxylate transporter MCT1 is located at the basolateral pole of rat jejunum."
    Orsenigo M.N., Tosco M., Bazzini C., Laforenza U., Faelli A.
    Exp. Physiol. 84:1033-1042(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 264-472, FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
    Tissue: Jejunal epithelium.
  5. "Interaction of the erythrocyte lactate transporter (monocarboxylate transporter 1) with an integral 70-kDa membrane glycoprotein of the immunoglobulin superfamily."
    Poole R.C., Halestrap A.P.
    J. Biol. Chem. 272:14624-14628(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH EMB, TISSUE SPECIFICITY, SUBCELLULAR LOCATION.
  6. "Fluorescence resonance energy transfer studies on the interaction between the lactate transporter MCT1 and CD147 provide information on the topology and stoichiometry of the complex in situ."
    Wilson M.C., Meredith D., Halestrap A.P.
    J. Biol. Chem. 277:3666-3672(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH BSG, FUNCTION, SUBCELLULAR LOCATION.
  7. "Phosphoproteomic analysis of rat liver by high capacity IMAC and LC-MS/MS."
    Moser K., White F.M.
    J. Proteome Res. 5:98-104(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-213, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  8. "The role of charged residues in the transmembrane helices of monocarboxylate transporter 1 and its ancillary protein basigin in determining plasma membrane expression and catalytic activity."
    Manoharan C., Wilson M.C., Sessions R.B., Halestrap A.P.
    Mol. Membr. Biol. 23:486-498(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH BSG, TOPOLOGY, 3D-STRUCTURE MODELING, MUTAGENESIS OF ARG-86; ARG-196; ASP-302 AND ARG-306.
  9. "Studies on the DIDS-binding site of monocarboxylate transporter 1 suggest a homology model of the open conformation and a plausible translocation cycle."
    Wilson M.C., Meredith D., Bunnun C., Sessions R.B., Halestrap A.P.
    J. Biol. Chem. 284:20011-20021(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH EMB, SUBCELLULAR LOCATION, TOPOLOGY, 3D-STRUCTURE MODELING, MUTAGENESIS OF LYS-38; LYS-45; LYS-282; LYS-284; LYS-290 AND LYS-413, ENZYME REGULATION, TISSUE SPECIFICITY.

Entry informationi

Entry nameiMOT1_RAT
AccessioniPrimary (citable) accession number: P53987
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: September 3, 2014
This is version 112 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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