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Protein

Monocarboxylate transporter 1

Gene

Slc16a1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Proton-coupled monocarboxylate transporter. Catalyzes the rapid transport across the plasma membrane of many monocarboxylates such as lactate, pyruvate, branched-chain oxo acids derived from leucine, valine and isoleucine, and the ketone bodies acetoacetate, beta-hydroxybutyrate and acetate. Depending on the tissue and on cicumstances, mediates the import or export of lactic acid and ketone bodies. Required for normal nutrient assimilation, increase of white adipose tissue and body weight gain when on a high-fat diet. Plays a role in cellular responses to a high-fat diet by modulating the cellular levels of lactate and pyruvate, small molecules that contribute to the regulation of central metabolic pathways and insulin secretion, with concomitant effects on plasma insulin levels and blood glucose homeostasis.2 Publications

GO - Molecular functioni

GO - Biological processi

  • behavioral response to nutrient Source: UniProtKB
  • cellular response to organic cyclic compound Source: Ensembl
  • centrosome organization Source: MGI
  • glucose homeostasis Source: UniProtKB
  • lipid metabolic process Source: UniProtKB
  • plasma membrane lactate transport Source: UniProtKB
  • regulation of insulin secretion Source: UniProtKB
  • response to food Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Symport, Transport

Enzyme and pathway databases

ReactomeiR-MMU-210991. Basigin interactions.
R-MMU-433692. Proton-coupled monocarboxylate transport.
R-MMU-70268. Pyruvate metabolism.
SABIO-RKP53986.

Names & Taxonomyi

Protein namesi
Recommended name:
Monocarboxylate transporter 1
Short name:
MCT 1
Alternative name(s):
Solute carrier family 16 member 1
Gene namesi
Name:Slc16a1
Synonyms:Mct1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 3

Organism-specific databases

MGIiMGI:106013. Slc16a1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 1515CytoplasmicSequence analysisAdd
BLAST
Transmembranei16 – 3621HelicalSequence analysisAdd
BLAST
Topological domaini37 – 5923ExtracellularSequence analysisAdd
BLAST
Transmembranei60 – 8021HelicalSequence analysisAdd
BLAST
Topological domaini81 – 866CytoplasmicSequence analysis
Transmembranei87 – 10721HelicalSequence analysisAdd
BLAST
Topological domaini108 – 1114ExtracellularSequence analysis
Transmembranei112 – 13221HelicalSequence analysisAdd
BLAST
Topological domaini133 – 14311CytoplasmicSequence analysisAdd
BLAST
Transmembranei144 – 16421HelicalSequence analysisAdd
BLAST
Topological domaini165 – 1662ExtracellularSequence analysis
Transmembranei167 – 18721HelicalSequence analysisAdd
BLAST
Topological domaini188 – 25568CytoplasmicSequence analysisAdd
BLAST
Transmembranei256 – 27621HelicalSequence analysisAdd
BLAST
Topological domaini277 – 29115ExtracellularSequence analysisAdd
BLAST
Transmembranei292 – 31221HelicalSequence analysisAdd
BLAST
Topological domaini313 – 3219CytoplasmicSequence analysis
Transmembranei322 – 34221HelicalSequence analysisAdd
BLAST
Topological domaini343 – 3464ExtracellularSequence analysis
Transmembranei347 – 36721HelicalSequence analysisAdd
BLAST
Topological domaini368 – 38215CytoplasmicSequence analysisAdd
BLAST
Transmembranei383 – 40321HelicalSequence analysisAdd
BLAST
Topological domaini404 – 41512ExtracellularSequence analysisAdd
BLAST
Transmembranei416 – 43621HelicalSequence analysisAdd
BLAST
Topological domaini437 – 49357CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Complete lethality during early embryonic development. Heterozygous mice are viable and show no obvious morphological or behavorial phenotype. Heterozygous mice that are kept on a normal diet show normal weight gain, normal glucose tolerance and insulin sensitivity. In contrast, mice exhibit a striking reduction in weight gain relative to wild-type, when kept on a high-fat diet. This is due to reduced fat accumulation in the liver and in subcutaneous white adipose tissue. In addition, heterozygous mice kept on a high-fat diet show higher glucose tolerance and higher insulin sensitivity than wild-type. The reduced weight gain is explained by reduced food intake, less efficient nutrient assimilation in the intestine and increased oxygen consumption when kept on a high-fat diet. Besides, heterozygous mice fail to respond to a high-fat diet by up-regulation of genes involved in lipid metabolism.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 493493Monocarboxylate transporter 1PRO_0000211383Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei210 – 2101PhosphoserineCombined sources
Modified residuei213 – 2131PhosphoserineCombined sources
Modified residuei220 – 2201PhosphoserineCombined sources
Modified residuei224 – 2241PhosphothreonineCombined sources
Modified residuei230 – 2301PhosphoserineCombined sources
Modified residuei461 – 4611PhosphoserineCombined sources
Modified residuei462 – 4621PhosphothreonineCombined sources
Modified residuei477 – 4771PhosphoserineCombined sources
Modified residuei482 – 4821PhosphoserineCombined sources
Modified residuei483 – 4831PhosphoserineCombined sources
Modified residuei491 – 4911PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiP53986.
PaxDbiP53986.
PRIDEiP53986.

PTM databases

iPTMnetiP53986.
PhosphoSiteiP53986.
SwissPalmiP53986.

Expressioni

Tissue specificityi

Detected in liver, brain, spinal cord, spermatozoa, muscle, white adipose tissue and brown adipose tissue (at protein level). Widely expressed, except in pancreas, where expression is not detectable.5 Publications

Gene expression databases

BgeeiP53986.
ExpressionAtlasiP53986. baseline and differential.
GenevisibleiP53986. MM.

Interactioni

Subunit structurei

Interacts with BSG. Interacts with EMB. Interaction with either BSG or EMB is required for expression at the cell membrane.1 Publication

Protein-protein interaction databases

BioGridi203282. 2 interactions.
STRINGi10090.ENSMUSP00000045216.

Structurei

3D structure databases

ProteinModelPortaliP53986.
SMRiP53986. Positions 40-86, 384-411.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IMVV. Eukaryota.
ENOG41116NW. LUCA.
HOGENOMiHOG000280688.
HOVERGENiHBG006384.
InParanoidiP53986.
KOiK08179.
OMAiYSIFGWR.
OrthoDBiEOG7W9RTN.
PhylomeDBiP53986.
TreeFamiTF313792.

Family and domain databases

InterProiIPR004743. MCT.
IPR030757. MCT1.
IPR011701. MFS.
IPR020846. MFS_dom.
[Graphical view]
PANTHERiPTHR11360:SF24. PTHR11360:SF24. 1 hit.
PfamiPF07690. MFS_1. 1 hit.
[Graphical view]
SUPFAMiSSF103473. SSF103473. 2 hits.
TIGRFAMsiTIGR00892. 2A0113. 1 hit.
PROSITEiPS50850. MFS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P53986-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPPAIGGPVG YTPPDGGWGW AVLVGAFISI GFSYAFPKSI TVFFKEIEVI
60 70 80 90 100
FSATTSEVSW ISSIMLAVMY AGGPISSILV NKYGSRPVMI AGGCLSGCGL
110 120 130 140 150
IAASFCNTVQ ELYLCIGVIG GLGLAFNLNP ALTMIGKYFY KKRPLANGLA
160 170 180 190 200
MAGSPVFLST LAPLNQAFFD IFDWRGSFLI LGGLLLNCCV AGSLMRPIGP
210 220 230 240 250
EQVKLEKLKS KESLQEAGKS DANTDLIGGS PKGEKLSVFQ TINKFLDLSL
260 270 280 290 300
FTHRGFLLYL SGNVVMFFGL FTPLVFLSSY GKSKDFSSEK SAFLLSILAF
310 320 330 340 350
VDMVARPSMG LAANTKWIRP RIQYFFAASV VANGVCHLLA PLSTTYVGFC
360 370 380 390 400
VYAGVFGFAF GWLSSVLFET LMDLIGPQRF SSAVGLVTIV ECCPVLLGPP
410 420 430 440 450
LLGRLNDMYG DYKYTYWACG VILIIAGIYL FIGMGINYRL LAKEQKAEEK
460 470 480 490
QKREGKEDEA STDVDEKPKE TMKAAQSPQQ HSSGDPTEEE SPV
Length:493
Mass (Da):53,267
Last modified:October 1, 1996 - v1
Checksum:i8B6DAB7741340DD7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X82438 mRNA. Translation: CAA57819.1.
AF058055 mRNA. Translation: AAC13720.1.
BC014777 mRNA. Translation: AAH14777.1.
CCDSiCCDS17702.1.
RefSeqiNP_033222.1. NM_009196.4.
UniGeneiMm.9086.

Genome annotation databases

EnsembliENSMUST00000046212; ENSMUSP00000045216; ENSMUSG00000032902.
GeneIDi20501.
KEGGimmu:20501.
UCSCiuc008qui.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X82438 mRNA. Translation: CAA57819.1.
AF058055 mRNA. Translation: AAC13720.1.
BC014777 mRNA. Translation: AAH14777.1.
CCDSiCCDS17702.1.
RefSeqiNP_033222.1. NM_009196.4.
UniGeneiMm.9086.

3D structure databases

ProteinModelPortaliP53986.
SMRiP53986. Positions 40-86, 384-411.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi203282. 2 interactions.
STRINGi10090.ENSMUSP00000045216.

PTM databases

iPTMnetiP53986.
PhosphoSiteiP53986.
SwissPalmiP53986.

Proteomic databases

EPDiP53986.
PaxDbiP53986.
PRIDEiP53986.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000046212; ENSMUSP00000045216; ENSMUSG00000032902.
GeneIDi20501.
KEGGimmu:20501.
UCSCiuc008qui.2. mouse.

Organism-specific databases

CTDi6566.
MGIiMGI:106013. Slc16a1.

Phylogenomic databases

eggNOGiENOG410IMVV. Eukaryota.
ENOG41116NW. LUCA.
HOGENOMiHOG000280688.
HOVERGENiHBG006384.
InParanoidiP53986.
KOiK08179.
OMAiYSIFGWR.
OrthoDBiEOG7W9RTN.
PhylomeDBiP53986.
TreeFamiTF313792.

Enzyme and pathway databases

ReactomeiR-MMU-210991. Basigin interactions.
R-MMU-433692. Proton-coupled monocarboxylate transport.
R-MMU-70268. Pyruvate metabolism.
SABIO-RKP53986.

Miscellaneous databases

NextBioi298671.
PROiP53986.
SOURCEiSearch...

Gene expression databases

BgeeiP53986.
ExpressionAtlasiP53986. baseline and differential.
GenevisibleiP53986. MM.

Family and domain databases

InterProiIPR004743. MCT.
IPR030757. MCT1.
IPR011701. MFS.
IPR020846. MFS_dom.
[Graphical view]
PANTHERiPTHR11360:SF24. PTHR11360:SF24. 1 hit.
PfamiPF07690. MFS_1. 1 hit.
[Graphical view]
SUPFAMiSSF103473. SSF103473. 2 hits.
TIGRFAMsiTIGR00892. 2A0113. 1 hit.
PROSITEiPS50850. MFS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and sequencing of the monocarboxylate transporter from mouse Ehrlich Lettre tumour cell confirms its identity as MCT1 and demonstrates that glycosylation is not required for MCT1 function."
    Carpenter L., Poole R.C., Halestrap A.P.
    Biochim. Biophys. Acta 1279:157-163(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
  2. "Monocarboxylate transporter expression in mouse brain."
    Koehler-Stec E.M., Simpson I.A., Vannucci S.J., Landschulz K.T., Landschulz W.H.
    Am. J. Physiol. 275:E516-E524(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
    Strain: 129.
    Tissue: Kidney.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Mammary gland.
  4. "Qualitative and quantitative analyses of protein phosphorylation in naive and stimulated mouse synaptosomal preparations."
    Munton R.P., Tweedie-Cullen R., Livingstone-Zatchej M., Weinandy F., Waidelich M., Longo D., Gehrig P., Potthast F., Rutishauser D., Gerrits B., Panse C., Schlapbach R., Mansuy I.M.
    Mol. Cell. Proteomics 6:283-293(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain cortex.
  5. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-210; THR-224 AND SER-491, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.
  6. "Specific phosphopeptide enrichment with immobilized titanium ion affinity chromatography adsorbent for phosphoproteome analysis."
    Zhou H., Ye M., Dong J., Han G., Jiang X., Wu R., Zou H.
    J. Proteome Res. 7:3957-3967(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-213, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.
  7. "The phagosomal proteome in interferon-gamma-activated macrophages."
    Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
    Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-213; SER-230; SER-461; THR-462; SER-477 AND SER-491, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  8. "Large scale localization of protein phosphorylation by use of electron capture dissociation mass spectrometry."
    Sweet S.M., Bailey C.M., Cunningham D.L., Heath J.K., Cooper H.J.
    Mol. Cell. Proteomics 8:904-912(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-213, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Embryonic fibroblast.
  9. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-210; SER-213; SER-220; SER-230; SER-477; SER-482; SER-483 AND SER-491, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Brown adipose tissue, Heart, Kidney, Liver, Lung, Pancreas, Spleen and Testis.
  10. "Overexpression of monocarboxylate transporter-1 (SLC16A1) in mouse pancreatic beta-cells leads to relative hyperinsulinism during exercise."
    Pullen T.J., Sylow L., Sun G., Halestrap A.P., Richter E.A., Rutter G.A.
    Diabetes 61:1719-1725(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY.
  11. "The monocarboxylate transporter family--role and regulation."
    Halestrap A.P., Wilson M.C.
    IUBMB Life 64:109-119(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: REVIEW.
  12. "Basigin interacts with both MCT1 and MCT2 in murine spermatozoa."
    Mannowetz N., Wandernoth P., Wennemuth G.
    J. Cell. Physiol. 227:2154-2162(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH BSG, TISSUE SPECIFICITY, SUBCELLULAR LOCATION.
  13. "Resistance to diet-induced obesity and associated metabolic perturbations in haploinsufficient monocarboxylate transporter 1 mice."
    Lengacher S., Nehiri-Sitayeb T., Steiner N., Carneiro L., Favrod C., Preitner F., Thorens B., Stehle J.C., Dix L., Pralong F., Magistretti P.J., Pellerin L.
    PLoS ONE 8:E82505-E82505(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISRUPTION PHENOTYPE, FUNCTION, TISSUE SPECIFICITY.

Entry informationi

Entry nameiMOT1_MOUSE
AccessioniPrimary (citable) accession number: P53986
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: May 11, 2016
This is version 136 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

Overexpression in pancreatic beta-cells triggers insulin secretion in response to pyruvate, causing hyperinsulemia and hypoglycemia during strenuous exercise.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.