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Protein

Monocarboxylate transporter 1

Gene

Slc16a1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Proton-coupled monocarboxylate transporter. Catalyzes the rapid transport across the plasma membrane of many monocarboxylates such as lactate, pyruvate, branched-chain oxo acids derived from leucine, valine and isoleucine, and the ketone bodies acetoacetate, beta-hydroxybutyrate and acetate. Depending on the tissue and on cicumstances, mediates the import or export of lactic acid and ketone bodies. Required for normal nutrient assimilation, increase of white adipose tissue and body weight gain when on a high-fat diet. Plays a role in cellular responses to a high-fat diet by modulating the cellular levels of lactate and pyruvate, small molecules that contribute to the regulation of central metabolic pathways and insulin secretion, with concomitant effects on plasma insulin levels and blood glucose homeostasis.2 Publications

Miscellaneous

Overexpression in pancreatic beta-cells triggers insulin secretion in response to pyruvate, causing hyperinsulemia and hypoglycemia during strenuous exercise.

GO - Molecular functioni

GO - Biological processi

  • behavioral response to nutrient Source: UniProtKB
  • cellular response to organic cyclic compound Source: Ensembl
  • centrosome cycle Source: MGI
  • glucose homeostasis Source: UniProtKB
  • lipid metabolic process Source: UniProtKB
  • plasma membrane lactate transport Source: UniProtKB
  • regulation of insulin secretion Source: UniProtKB
  • response to food Source: UniProtKB

Keywordsi

Biological processSymport, Transport

Enzyme and pathway databases

ReactomeiR-MMU-210991 Basigin interactions
R-MMU-433692 Proton-coupled monocarboxylate transport
R-MMU-70268 Pyruvate metabolism
SABIO-RKP53986

Names & Taxonomyi

Protein namesi
Recommended name:
Monocarboxylate transporter 1
Short name:
MCT 1
Alternative name(s):
Solute carrier family 16 member 1
Gene namesi
Name:Slc16a1
Synonyms:Mct1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 3

Organism-specific databases

MGIiMGI:106013 Slc16a1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 15CytoplasmicSequence analysisAdd BLAST15
Transmembranei16 – 36HelicalSequence analysisAdd BLAST21
Topological domaini37 – 59ExtracellularSequence analysisAdd BLAST23
Transmembranei60 – 80HelicalSequence analysisAdd BLAST21
Topological domaini81 – 86CytoplasmicSequence analysis6
Transmembranei87 – 107HelicalSequence analysisAdd BLAST21
Topological domaini108 – 111ExtracellularSequence analysis4
Transmembranei112 – 132HelicalSequence analysisAdd BLAST21
Topological domaini133 – 143CytoplasmicSequence analysisAdd BLAST11
Transmembranei144 – 164HelicalSequence analysisAdd BLAST21
Topological domaini165 – 166ExtracellularSequence analysis2
Transmembranei167 – 187HelicalSequence analysisAdd BLAST21
Topological domaini188 – 255CytoplasmicSequence analysisAdd BLAST68
Transmembranei256 – 276HelicalSequence analysisAdd BLAST21
Topological domaini277 – 291ExtracellularSequence analysisAdd BLAST15
Transmembranei292 – 312HelicalSequence analysisAdd BLAST21
Topological domaini313 – 321CytoplasmicSequence analysis9
Transmembranei322 – 342HelicalSequence analysisAdd BLAST21
Topological domaini343 – 346ExtracellularSequence analysis4
Transmembranei347 – 367HelicalSequence analysisAdd BLAST21
Topological domaini368 – 382CytoplasmicSequence analysisAdd BLAST15
Transmembranei383 – 403HelicalSequence analysisAdd BLAST21
Topological domaini404 – 415ExtracellularSequence analysisAdd BLAST12
Transmembranei416 – 436HelicalSequence analysisAdd BLAST21
Topological domaini437 – 493CytoplasmicSequence analysisAdd BLAST57

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Complete lethality during early embryonic development. Heterozygous mice are viable and show no obvious morphological or behavorial phenotype. Heterozygous mice that are kept on a normal diet show normal weight gain, normal glucose tolerance and insulin sensitivity. In contrast, mice exhibit a striking reduction in weight gain relative to wild-type, when kept on a high-fat diet. This is due to reduced fat accumulation in the liver and in subcutaneous white adipose tissue. In addition, heterozygous mice kept on a high-fat diet show higher glucose tolerance and higher insulin sensitivity than wild-type. The reduced weight gain is explained by reduced food intake, less efficient nutrient assimilation in the intestine and increased oxygen consumption when kept on a high-fat diet. Besides, heterozygous mice fail to respond to a high-fat diet by up-regulation of genes involved in lipid metabolism.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002113831 – 493Monocarboxylate transporter 1Add BLAST493

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei210PhosphoserineCombined sources1
Modified residuei213PhosphoserineCombined sources1
Modified residuei220PhosphoserineCombined sources1
Modified residuei224PhosphothreonineCombined sources1
Modified residuei230PhosphoserineCombined sources1
Modified residuei461PhosphoserineCombined sources1
Modified residuei462PhosphothreonineCombined sources1
Modified residuei477PhosphoserineCombined sources1
Modified residuei482PhosphoserineCombined sources1
Modified residuei483PhosphoserineCombined sources1
Modified residuei491PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiP53986
PaxDbiP53986
PeptideAtlasiP53986
PRIDEiP53986

PTM databases

iPTMnetiP53986
PhosphoSitePlusiP53986
SwissPalmiP53986

Expressioni

Tissue specificityi

Detected in liver, brain, spinal cord, spermatozoa, muscle, white adipose tissue and brown adipose tissue (at protein level). Widely expressed, except in pancreas, where expression is not detectable.5 Publications

Gene expression databases

BgeeiENSMUSG00000032902
ExpressionAtlasiP53986 baseline and differential
GenevisibleiP53986 MM

Interactioni

Subunit structurei

Interacts with BSG. Interacts with EMB. Interaction with either BSG or EMB is required for expression at the cell membrane.1 Publication

GO - Molecular functioni

Protein-protein interaction databases

BioGridi203282, 3 interactors
STRINGi10090.ENSMUSP00000045216

Structurei

3D structure databases

ProteinModelPortaliP53986
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IMVV Eukaryota
ENOG41116NW LUCA
GeneTreeiENSGT00760000118926
HOGENOMiHOG000280688
HOVERGENiHBG006384
InParanoidiP53986
KOiK08179
OMAiRPFVGMF
OrthoDBiEOG091G05SW
PhylomeDBiP53986
TreeFamiTF313792

Family and domain databases

CDDicd06174 MFS, 1 hit
InterProiView protein in InterPro
IPR004743 MCT
IPR030757 MCT1
IPR011701 MFS
IPR020846 MFS_dom
IPR036259 MFS_trans_sf
PANTHERiPTHR11360:SF24 PTHR11360:SF24, 1 hit
PfamiView protein in Pfam
PF07690 MFS_1, 1 hit
SUPFAMiSSF103473 SSF103473, 2 hits
TIGRFAMsiTIGR00892 2A0113, 1 hit
PROSITEiView protein in PROSITE
PS50850 MFS, 1 hit

Sequencei

Sequence statusi: Complete.

P53986-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPPAIGGPVG YTPPDGGWGW AVLVGAFISI GFSYAFPKSI TVFFKEIEVI
60 70 80 90 100
FSATTSEVSW ISSIMLAVMY AGGPISSILV NKYGSRPVMI AGGCLSGCGL
110 120 130 140 150
IAASFCNTVQ ELYLCIGVIG GLGLAFNLNP ALTMIGKYFY KKRPLANGLA
160 170 180 190 200
MAGSPVFLST LAPLNQAFFD IFDWRGSFLI LGGLLLNCCV AGSLMRPIGP
210 220 230 240 250
EQVKLEKLKS KESLQEAGKS DANTDLIGGS PKGEKLSVFQ TINKFLDLSL
260 270 280 290 300
FTHRGFLLYL SGNVVMFFGL FTPLVFLSSY GKSKDFSSEK SAFLLSILAF
310 320 330 340 350
VDMVARPSMG LAANTKWIRP RIQYFFAASV VANGVCHLLA PLSTTYVGFC
360 370 380 390 400
VYAGVFGFAF GWLSSVLFET LMDLIGPQRF SSAVGLVTIV ECCPVLLGPP
410 420 430 440 450
LLGRLNDMYG DYKYTYWACG VILIIAGIYL FIGMGINYRL LAKEQKAEEK
460 470 480 490
QKREGKEDEA STDVDEKPKE TMKAAQSPQQ HSSGDPTEEE SPV
Length:493
Mass (Da):53,267
Last modified:October 1, 1996 - v1
Checksum:i8B6DAB7741340DD7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X82438 mRNA Translation: CAA57819.1
AF058055 mRNA Translation: AAC13720.1
BC014777 mRNA Translation: AAH14777.1
CCDSiCCDS17702.1
RefSeqiNP_033222.1, NM_009196.4
UniGeneiMm.9086

Genome annotation databases

EnsembliENSMUST00000046212; ENSMUSP00000045216; ENSMUSG00000032902
GeneIDi20501
KEGGimmu:20501
UCSCiuc008qui.2 mouse

Similar proteinsi

Entry informationi

Entry nameiMOT1_MOUSE
AccessioniPrimary (citable) accession number: P53986
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: May 23, 2018
This is version 150 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health