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Protein

Monocarboxylate transporter 1

Gene

SLC16A1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Proton-coupled monocarboxylate transporter. Catalyzes the rapid transport across the plasma membrane of many monocarboxylates such as lactate, pyruvate, branched-chain oxo acids derived from leucine, valine and isoleucine, and the ketone bodies acetoacetate, beta-hydroxybutyrate and acetate. Depending on the tissue and on cicumstances, mediates the import or export of lactic acid and ketone bodies. Required for normal nutrient assimilation, increase of white adipose tissue and body weight gain when on a high-fat diet. Plays a role in cellular responses to a high-fat diet by modulating the cellular levels of lactate and pyruvate, small molecules that contribute to the regulation of central metabolic pathways and insulin secretion, with concomitant effects on plasma insulin levels and blood glucose homeostasis.1 Publication

GO - Molecular functioni

GO - Biological processi

  • behavioral response to nutrient Source: Ensembl
  • cellular response to organic cyclic compound Source: Ensembl
  • centrosome organization Source: UniProtKB
  • glucose homeostasis Source: Ensembl
  • leukocyte migration Source: Reactome
  • lipid metabolic process Source: Ensembl
  • mevalonate transport Source: ProtInc
  • monocarboxylic acid transport Source: ProtInc
  • plasma membrane lactate transport Source: UniProtKB
  • pyruvate metabolic process Source: Reactome
  • regulation of insulin secretion Source: Ensembl
  • response to food Source: Ensembl
Complete GO annotation...

Keywords - Biological processi

Symport, Transport

Enzyme and pathway databases

BioCyciMetaCyc:ENSG00000155380-MONOMER.
ZFISH:ENSG00000155380-MONOMER.
ReactomeiR-HSA-210991. Basigin interactions.
R-HSA-433692. Proton-coupled monocarboxylate transport.
R-HSA-70268. Pyruvate metabolism.
SABIO-RKP53985.

Protein family/group databases

TCDBi2.A.1.13.1. the major facilitator superfamily (mfs).

Names & Taxonomyi

Protein namesi
Recommended name:
Monocarboxylate transporter 1
Short name:
MCT 1
Alternative name(s):
Solute carrier family 16 member 1
Gene namesi
Name:SLC16A1
Synonyms:MCT1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 1

Organism-specific databases

HGNCiHGNC:10922. SLC16A1.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 15CytoplasmicSequence analysisAdd BLAST15
Transmembranei16 – 36HelicalSequence analysisAdd BLAST21
Topological domaini37 – 59ExtracellularSequence analysisAdd BLAST23
Transmembranei60 – 80HelicalSequence analysisAdd BLAST21
Topological domaini81 – 86CytoplasmicSequence analysis6
Transmembranei87 – 107HelicalSequence analysisAdd BLAST21
Topological domaini108 – 111ExtracellularSequence analysis4
Transmembranei112 – 132HelicalSequence analysisAdd BLAST21
Topological domaini133 – 143CytoplasmicSequence analysisAdd BLAST11
Transmembranei144 – 164HelicalSequence analysisAdd BLAST21
Topological domaini165 – 166ExtracellularSequence analysis2
Transmembranei167 – 187HelicalSequence analysisAdd BLAST21
Topological domaini188 – 262CytoplasmicSequence analysisAdd BLAST75
Transmembranei263 – 283HelicalSequence analysisAdd BLAST21
Topological domaini284 – 298ExtracellularSequence analysisAdd BLAST15
Transmembranei299 – 319HelicalSequence analysisAdd BLAST21
Topological domaini320 – 328CytoplasmicSequence analysis9
Transmembranei329 – 349HelicalSequence analysisAdd BLAST21
Topological domaini350 – 353ExtracellularSequence analysis4
Transmembranei354 – 374HelicalSequence analysisAdd BLAST21
Topological domaini375 – 389CytoplasmicSequence analysisAdd BLAST15
Transmembranei390 – 410HelicalSequence analysisAdd BLAST21
Topological domaini411 – 422ExtracellularSequence analysisAdd BLAST12
Transmembranei423 – 443HelicalSequence analysisAdd BLAST21
Topological domaini444 – 500CytoplasmicSequence analysisAdd BLAST57

GO - Cellular componenti

  • centrosome Source: UniProtKB
  • extracellular exosome Source: UniProtKB
  • integral component of membrane Source: ProtInc
  • integral component of plasma membrane Source: UniProtKB
  • membrane Source: UniProtKB
  • mitochondrion Source: Ensembl
  • plasma membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Involvement in diseasei

Symptomatic deficiency in lactate transport (SDLT)1 Publication
The disease is caused by mutations affecting the gene represented in this entry.
Disease descriptionDeficiency of lactate transporter may result in an acidic intracellular environment created by muscle activity with consequent degeneration of muscle and release of myoglobin and creatine kinase. This defect might compromise extreme performance in otherwise healthy individuals.
See also OMIM:245340
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_010434204K → E in SDLT. 1 PublicationCorresponds to variant rs80358222dbSNPEnsembl.1
Natural variantiVAR_010435472G → R in SDLT. 1 PublicationCorresponds to variant rs72552271dbSNPEnsembl.1
Familial hyperinsulinemic hypoglycemia 7 (HHF7)1 Publication
The disease is caused by mutations affecting the gene represented in this entry.
Disease descriptionDominantly inherited hypoglycemic disorder characterized by inappropriate insulin secretion during anaerobic exercise or on pyruvate load.
See also OMIM:610021
Monocarboxylate transporter 1 deficiency (MCT1D)1 Publication
The disease is caused by mutations affecting the gene represented in this entry.
Disease descriptionA metabolic disorder characterized by recurrent ketoacidosis, a pathologic state due to ketone formation exceeding ketone utilization. The clinical consequences of ketoacidosis are vomiting, osmotic diuresis, dehydration, and Kussmaul breathing. The condition may progress to decreased consciousness and, ultimately, death.
See also OMIM:616095
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_072428313R → Q in MCT1D. 1 PublicationCorresponds to variant rs606231302dbSNPEnsembl.1

Keywords - Diseasei

Disease mutation

Organism-specific databases

DisGeNETi6566.
MalaCardsiSLC16A1.
MIMi245340. phenotype.
610021. phenotype.
616095. phenotype.
OpenTargetsiENSG00000155380.
ENSG00000281917.
Orphaneti165991. Exercise-induced hyperinsulinism.
171690. Metabolic myopathy due to lactate transporter defect.
PharmGKBiPA35813.

Chemistry databases

ChEMBLiCHEMBL4360.
DrugBankiDB03166. Acetic acid.
DB00345. Aminohippurate.
DB00415. Ampicillin.
DB03793. Benzoic Acid.
DB00529. Foscarnet.
DB01440. Gamma Hydroxybutyric Acid.
DB04398. Lactic Acid.
DB00563. Methotrexate.
DB00731. Nateglinide.
DB00627. Niacin.
DB04552. Niflumic Acid.
DB00175. Pravastatin.
DB01032. Probenecid.
DB00119. Pyruvic acid.
DB00936. Salicylic acid.
DB00313. Valproic Acid.
GuidetoPHARMACOLOGYi988.

Polymorphism and mutation databases

BioMutaiSLC16A1.
DMDMi313104214.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002113811 – 500Monocarboxylate transporter 1Add BLAST500

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei210PhosphoserineBy similarity1
Modified residuei213PhosphoserineCombined sources1
Modified residuei231PhosphothreonineBy similarity1
Modified residuei461PhosphoserineCombined sources1
Modified residuei466PhosphothreonineCombined sources1
Modified residuei467PhosphoserineCombined sources1
Modified residuei483PhosphoserineCombined sources1
Modified residuei498PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiP53985.
MaxQBiP53985.
PaxDbiP53985.
PeptideAtlasiP53985.
PRIDEiP53985.

PTM databases

iPTMnetiP53985.
PhosphoSitePlusiP53985.
SwissPalmiP53985.

Miscellaneous databases

PMAP-CutDBP53985.

Expressioni

Tissue specificityi

Detected in heart and in blood lymphocytes and monocytes (at protein level). Widely expressed.1 Publication

Gene expression databases

BgeeiENSG00000155380.
CleanExiHS_SLC16A1.
ExpressionAtlasiP53985. baseline and differential.
GenevisibleiP53985. HS.

Organism-specific databases

HPAiCAB017489.
HPA003324.

Interactioni

Subunit structurei

Interacts with EMB. Interaction with either BSG or EMB is required for expression at the cell membrane (By similarity). Interacts with BSG; this is required for expression at the cell membrane.By similarity1 Publication

Protein-protein interaction databases

BioGridi112454. 24 interactors.
IntActiP53985. 12 interactors.
MINTiMINT-5004345.
STRINGi9606.ENSP00000358640.

Chemistry databases

BindingDBiP53985.

Structurei

3D structure databases

ProteinModelPortaliP53985.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2504. Eukaryota.
COG0477. LUCA.
GeneTreeiENSGT00760000118926.
HOGENOMiHOG000280688.
HOVERGENiHBG006384.
InParanoidiP53985.
KOiK08179.
OMAiHMLAPLS.
OrthoDBiEOG091G05SW.
PhylomeDBiP53985.
TreeFamiTF313792.

Family and domain databases

CDDicd06174. MFS. 1 hit.
InterProiIPR004743. MCT.
IPR030757. MCT1.
IPR011701. MFS.
IPR020846. MFS_dom.
[Graphical view]
PANTHERiPTHR11360:SF24. PTHR11360:SF24. 1 hit.
PfamiPF07690. MFS_1. 1 hit.
[Graphical view]
SUPFAMiSSF103473. SSF103473. 2 hits.
TIGRFAMsiTIGR00892. 2A0113. 1 hit.
PROSITEiPS50850. MFS. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P53985-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MPPAVGGPVG YTPPDGGWGW AVVIGAFISI GFSYAFPKSI TVFFKEIEGI
60 70 80 90 100
FHATTSEVSW ISSIMLAVMY GGGPISSILV NKYGSRIVMI VGGCLSGCGL
110 120 130 140 150
IAASFCNTVQ QLYVCIGVIG GLGLAFNLNP ALTMIGKYFY KRRPLANGLA
160 170 180 190 200
MAGSPVFLCT LAPLNQVFFG IFGWRGSFLI LGGLLLNCCV AGALMRPIGP
210 220 230 240 250
KPTKAGKDKS KASLEKAGKS GVKKDLHDAN TDLIGRHPKQ EKRSVFQTIN
260 270 280 290 300
QFLDLTLFTH RGFLLYLSGN VIMFFGLFAP LVFLSSYGKS QHYSSEKSAF
310 320 330 340 350
LLSILAFVDM VARPSMGLVA NTKPIRPRIQ YFFAASVVAN GVCHMLAPLS
360 370 380 390 400
TTYVGFCVYA GFFGFAFGWL SSVLFETLMD LVGPQRFSSA VGLVTIVECC
410 420 430 440 450
PVLLGPPLLG RLNDMYGDYK YTYWACGVVL IISGIYLFIG MGINYRLLAK
460 470 480 490 500
EQKANEQKKE SKEEETSIDV AGKPNEVTKA AESPDQKDTD GGPKEEESPV
Length:500
Mass (Da):53,944
Last modified:November 30, 2010 - v3
Checksum:i3F5B048CB962ECC8
GO
Isoform 2 (identifier: P53985-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     411-500: RLNDMYGDYK...GGPKEEESPV → IVYLPTNVGLLQNKHVRWEC

Note: No experimental confirmation available.
Show »
Length:430
Mass (Da):46,234
Checksum:i28C6B55692E6C4BE
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti480A → T in AAC41707 (PubMed:7835905).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_05480485S → G.Corresponds to variant rs11551867dbSNPEnsembl.1
Natural variantiVAR_010434204K → E in SDLT. 1 PublicationCorresponds to variant rs80358222dbSNPEnsembl.1
Natural variantiVAR_072428313R → Q in MCT1D. 1 PublicationCorresponds to variant rs606231302dbSNPEnsembl.1
Natural variantiVAR_010435472G → R in SDLT. 1 PublicationCorresponds to variant rs72552271dbSNPEnsembl.1
Natural variantiVAR_010436490D → E.Combined sources3 PublicationsCorresponds to variant rs1049434dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_056191411 – 500RLNDM…EESPV → IVYLPTNVGLLQNKHVRWEC in isoform 2. 1 PublicationAdd BLAST90

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L31801 mRNA. Translation: AAC41707.1.
AJ438945 Genomic DNA. Translation: CAD27707.1.
AL162079 mRNA. Translation: CAB82412.1.
AL158844 Genomic DNA. Translation: CAI21872.1.
CH471122 Genomic DNA. Translation: EAW56552.1.
BC026317 mRNA. Translation: AAH26317.1.
BC045664 mRNA. Translation: AAH45664.1.
CCDSiCCDS858.1. [P53985-1]
PIRiA55568.
RefSeqiNP_001159968.1. NM_001166496.1. [P53985-1]
NP_003042.3. NM_003051.3. [P53985-1]
XP_011540328.1. XM_011542026.2. [P53985-1]
XP_011540329.1. XM_011542027.2. [P53985-1]
UniGeneiHs.75231.

Genome annotation databases

EnsembliENST00000369626; ENSP00000358640; ENSG00000155380. [P53985-1]
ENST00000538576; ENSP00000441065; ENSG00000155380. [P53985-1]
ENST00000628110; ENSP00000485688; ENSG00000281917. [P53985-1]
ENST00000630362; ENSP00000486000; ENSG00000281917. [P53985-1]
GeneIDi6566.
KEGGihsa:6566.
UCSCiuc001ecx.4. human. [P53985-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Web resourcesi

Atlas of Genetics and Cytogenetics in Oncology and Haematology

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L31801 mRNA. Translation: AAC41707.1.
AJ438945 Genomic DNA. Translation: CAD27707.1.
AL162079 mRNA. Translation: CAB82412.1.
AL158844 Genomic DNA. Translation: CAI21872.1.
CH471122 Genomic DNA. Translation: EAW56552.1.
BC026317 mRNA. Translation: AAH26317.1.
BC045664 mRNA. Translation: AAH45664.1.
CCDSiCCDS858.1. [P53985-1]
PIRiA55568.
RefSeqiNP_001159968.1. NM_001166496.1. [P53985-1]
NP_003042.3. NM_003051.3. [P53985-1]
XP_011540328.1. XM_011542026.2. [P53985-1]
XP_011540329.1. XM_011542027.2. [P53985-1]
UniGeneiHs.75231.

3D structure databases

ProteinModelPortaliP53985.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi112454. 24 interactors.
IntActiP53985. 12 interactors.
MINTiMINT-5004345.
STRINGi9606.ENSP00000358640.

Chemistry databases

BindingDBiP53985.
ChEMBLiCHEMBL4360.
DrugBankiDB03166. Acetic acid.
DB00345. Aminohippurate.
DB00415. Ampicillin.
DB03793. Benzoic Acid.
DB00529. Foscarnet.
DB01440. Gamma Hydroxybutyric Acid.
DB04398. Lactic Acid.
DB00563. Methotrexate.
DB00731. Nateglinide.
DB00627. Niacin.
DB04552. Niflumic Acid.
DB00175. Pravastatin.
DB01032. Probenecid.
DB00119. Pyruvic acid.
DB00936. Salicylic acid.
DB00313. Valproic Acid.
GuidetoPHARMACOLOGYi988.

Protein family/group databases

TCDBi2.A.1.13.1. the major facilitator superfamily (mfs).

PTM databases

iPTMnetiP53985.
PhosphoSitePlusiP53985.
SwissPalmiP53985.

Polymorphism and mutation databases

BioMutaiSLC16A1.
DMDMi313104214.

Proteomic databases

EPDiP53985.
MaxQBiP53985.
PaxDbiP53985.
PeptideAtlasiP53985.
PRIDEiP53985.

Protocols and materials databases

DNASUi6566.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000369626; ENSP00000358640; ENSG00000155380. [P53985-1]
ENST00000538576; ENSP00000441065; ENSG00000155380. [P53985-1]
ENST00000628110; ENSP00000485688; ENSG00000281917. [P53985-1]
ENST00000630362; ENSP00000486000; ENSG00000281917. [P53985-1]
GeneIDi6566.
KEGGihsa:6566.
UCSCiuc001ecx.4. human. [P53985-1]

Organism-specific databases

CTDi6566.
DisGeNETi6566.
GeneCardsiSLC16A1.
H-InvDBHIX0000897.
HGNCiHGNC:10922. SLC16A1.
HPAiCAB017489.
HPA003324.
MalaCardsiSLC16A1.
MIMi245340. phenotype.
600682. gene.
610021. phenotype.
616095. phenotype.
neXtProtiNX_P53985.
OpenTargetsiENSG00000155380.
ENSG00000281917.
Orphaneti165991. Exercise-induced hyperinsulinism.
171690. Metabolic myopathy due to lactate transporter defect.
PharmGKBiPA35813.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2504. Eukaryota.
COG0477. LUCA.
GeneTreeiENSGT00760000118926.
HOGENOMiHOG000280688.
HOVERGENiHBG006384.
InParanoidiP53985.
KOiK08179.
OMAiHMLAPLS.
OrthoDBiEOG091G05SW.
PhylomeDBiP53985.
TreeFamiTF313792.

Enzyme and pathway databases

BioCyciMetaCyc:ENSG00000155380-MONOMER.
ZFISH:ENSG00000155380-MONOMER.
ReactomeiR-HSA-210991. Basigin interactions.
R-HSA-433692. Proton-coupled monocarboxylate transport.
R-HSA-70268. Pyruvate metabolism.
SABIO-RKP53985.

Miscellaneous databases

ChiTaRSiSLC16A1. human.
GenomeRNAii6566.
PMAP-CutDBP53985.
PROiP53985.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000155380.
CleanExiHS_SLC16A1.
ExpressionAtlasiP53985. baseline and differential.
GenevisibleiP53985. HS.

Family and domain databases

CDDicd06174. MFS. 1 hit.
InterProiIPR004743. MCT.
IPR030757. MCT1.
IPR011701. MFS.
IPR020846. MFS_dom.
[Graphical view]
PANTHERiPTHR11360:SF24. PTHR11360:SF24. 1 hit.
PfamiPF07690. MFS_1. 1 hit.
[Graphical view]
SUPFAMiSSF103473. SSF103473. 2 hits.
TIGRFAMsiTIGR00892. 2A0113. 1 hit.
PROSITEiPS50850. MFS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMOT1_HUMAN
AccessioniPrimary (citable) accession number: P53985
Secondary accession number(s): Q49A45, Q5T8R6, Q9NSJ9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: November 30, 2010
Last modified: November 30, 2016
This is version 169 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Miscellaneous

Overexpression in pancreatic beta-cells triggers insulin secretion in response to pyruvate, causing hyperinsulemia and hypoglycemia during strenuous exercise.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.