Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P53983 (ASI3_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 87. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Protein ASI3
Alternative name(s):
Amino acid sensor-independent protein 3
Gene names
Name:ASI3
Ordered Locus Names:YNL008C
ORF Names:N2874
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length676 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Negative regulator of SPS-sensor signaling. Together with ASI1 and ASI2, prevents the unprocessed precursor forms of STP1 and STP2 that escape cytoplasmic anchoring from inducing SPS-sensor-regulated genes in the absence of inducing signals. Ref.5

Subunit structure

Interacts with ASI1. Ref.5

Subcellular location

Nucleus inner membrane; Multi-pass membrane protein Ref.3 Ref.5.

Sequence similarities

Contains 1 RING-type zinc finger.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 676676Protein ASI3
PRO_0000203467

Regions

Topological domain1 – 7878Perinuclear space Potential
Transmembrane79 – 9921Helical; Potential
Topological domain100 – 12627Nuclear Potential
Transmembrane127 – 14721Helical; Potential
Topological domain148 – 1569Perinuclear space Potential
Transmembrane157 – 17721Helical; Potential
Topological domain178 – 1814Nuclear Potential
Transmembrane182 – 20221Helical; Potential
Topological domain203 – 27775Perinuclear space Potential
Transmembrane278 – 29821Helical; Potential
Topological domain299 – 676378Nuclear Potential
Zinc finger624 – 66441RING-type; atypical

Amino acid modifications

Glycosylation241N-linked (GlcNAc...) Potential
Glycosylation341N-linked (GlcNAc...) Potential
Glycosylation461N-linked (GlcNAc...) Potential
Glycosylation661N-linked (GlcNAc...) Potential

Experimental info

Sequence conflict1 – 1515MSTNI…QLLHN → MLSNCSIT in CAA95867. Ref.1
Sequence conflict1 – 1515MSTNI…QLLHN → MLSNCSIT in CAA95868. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P53983 [UniParc].

Last modified September 21, 2011. Version 2.
Checksum: 58ECCEA014034A3B

FASTA67677,649
        10         20         30         40         50         60 
MSTNILQHVK QLLHNRDVFS FFHNKTGNLN YLDNTTQKPE VFVSPNSTIV SAPTLDSFQA 

        70         80         90        100        110        120 
LMEKGNFTTL QLAKVGIRMF FSYSVSKYAV LCFSTAIILN RLTVMSSLRS NSTNIRLPLW 

       130        140        150        160        170        180 
SKTLLHLVAT LSLVKALLQI LSQFGLMHEL HVSDTDFYAL SVYLFVALSD CIEIFISSTT 

       190        200        210        220        230        240 
NVPSLICSDF SIWGLSLNLY IISKMPAGQQ HIGDNVELLG AVFHRLVIHL VELFHIRAYR 

       250        260        270        280        290        300 
LCGEVILNAG FFTAFVTRTY LNGLDFINIC LIHNYFPGFF YISTILLASI GIFLKALFTS 

       310        320        330        340        350        360 
NPFRSLYSRY KNLEKWWRSN NYNGEEEFNE IALSLCLLLT SNDYKIFKKS DNVKSVDEVA 

       370        380        390        400        410        420 
AFSNSYVVSG HLNQLQSTPE DLLSRKEMTT DSQLPGFART YLGLFELVRT IILTYSRLLK 

       430        440        450        460        470        480 
NLLWSKNFES SIDKKPRVGK RKKRDLNKYV TEKNYKKFLY KPDVKELNIE SDLRSLELLL 

       490        500        510        520        530        540 
PEDDSSKDYF PPRKIDESVS DEEFDSDMES QLIIDEEKEL THLSSNAVDS DDLEEIAWNI 

       550        560        570        580        590        600 
SMWSILNYEM DVHNKVNGPL TRSQYGKRNP QGVLVDVVIE RLLHHTNSRY MYKRLNMKDD 

       610        620        630        640        650        660 
DKLEFKFDFA FDSCDEVEEM DLSCLICKVN KRNIVTWPCR CLALCDDCRI SLGYKGFATC 

       670 
VSCDSEVKGY SKLNIV 

« Hide

References

« Hide 'large scale' references
[1]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications."
Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J. expand/collapse author list , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.
Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]Saccharomyces Genome Database
Submitted (MAR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION, SEQUENCE REVISION.
Strain: ATCC 204508 / S288c.
[3]"Global analysis of protein localization in budding yeast."
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.
Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[4]"A global topology map of the Saccharomyces cerevisiae membrane proteome."
Kim H., Melen K., Oesterberg M., von Heijne G.
Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
Strain: ATCC 208353 / W303-1A.
[5]"Inner nuclear membrane proteins Asi1, Asi2, and Asi3 function in concert to maintain the latent properties of transcription factors Stp1 and Stp2."
Zargari A., Boban M., Heessen S., Andreasson C., Thyberg J., Ljungdahl P.O.
J. Biol. Chem. 282:594-605(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION, GLYCOSYLATION, INTERACTION WITH ASI1.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z71284 Genomic DNA. Translation: CAA95868.1.
Z71283 Genomic DNA. Translation: CAA95867.1.
BK006947 Genomic DNA. Translation: DAA10535.2.
PIRS62919.
RefSeqNP_014390.2. NM_001182847.2.

3D structure databases

ProteinModelPortalP53983.
SMRP53983. Positions 622-675.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid35817. 30 interactions.
DIPDIP-5065N.
IntActP53983. 5 interactions.
MINTMINT-543525.
STRING4932.YNL008C.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYNL008C; YNL008C; YNL008C.
GeneID855724.
KEGGsce:YNL008C.

Organism-specific databases

CYGDYNL008c.
SGDS000004953. ASI3.

Phylogenomic databases

eggNOGNOG46128.
GeneTreeENSGT00530000069536.
OMAIMIREEN.
OrthoDBEOG7BZW1Z.

Enzyme and pathway databases

BioCycYEAST:G3O-33050-MONOMER.

Gene expression databases

GenevestigatorP53983.

Family and domain databases

ProtoNetSearch...

Other

NextBio980093.

Entry information

Entry nameASI3_YEAST
AccessionPrimary (citable) accession number: P53983
Secondary accession number(s): D6W1G9
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: September 21, 2011
Last modified: April 16, 2014
This is version 87 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XIV

Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families