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Protein

Protein ASI3

Gene

ASI3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Negative regulator of SPS-sensor signaling. Together with ASI1 and ASI2, prevents the unprocessed precursor forms of STP1 and STP2 that escape cytoplasmic anchoring from inducing SPS-sensor-regulated genes in the absence of inducing signals.1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri624 – 66441RING-type; atypicalAdd
BLAST

GO - Molecular functioni

  • metal ion binding Source: UniProtKB-KW
  • ubiquitin-protein transferase activity Source: SGD

GO - Biological processi

  • cellular response to amino acid stimulus Source: SGD
  • protein ubiquitination Source: GOC
  • transcription factor catabolic process Source: SGD
  • ubiquitin-dependent protein catabolic process Source: SGD
Complete GO annotation...

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciYEAST:G3O-33050-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein ASI3
Alternative name(s):
Amino acid sensor-independent protein 3
Gene namesi
Name:ASI3
Ordered Locus Names:YNL008C
ORF Names:N2874
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIV

Organism-specific databases

EuPathDBiFungiDB:YNL008C.
SGDiS000004953. ASI3.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 7878Perinuclear spaceSequence analysisAdd
BLAST
Transmembranei79 – 9921HelicalSequence analysisAdd
BLAST
Topological domaini100 – 12627NuclearSequence analysisAdd
BLAST
Transmembranei127 – 14721HelicalSequence analysisAdd
BLAST
Topological domaini148 – 1569Perinuclear spaceSequence analysis
Transmembranei157 – 17721HelicalSequence analysisAdd
BLAST
Topological domaini178 – 1814NuclearSequence analysis
Transmembranei182 – 20221HelicalSequence analysisAdd
BLAST
Topological domaini203 – 27775Perinuclear spaceSequence analysisAdd
BLAST
Transmembranei278 – 29821HelicalSequence analysisAdd
BLAST
Topological domaini299 – 676378NuclearSequence analysisAdd
BLAST

GO - Cellular componenti

  • Asi complex Source: SGD
  • integral component of membrane Source: UniProtKB-KW
  • nuclear inner membrane Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 676676Protein ASI3PRO_0000203467Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi24 – 241N-linked (GlcNAc...)Sequence analysis
Glycosylationi34 – 341N-linked (GlcNAc...)Sequence analysis
Glycosylationi46 – 461N-linked (GlcNAc...)Sequence analysis
Glycosylationi66 – 661N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiP53983.

Interactioni

Subunit structurei

Interacts with ASI1.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
ASI1P540745EBI-28603,EBI-27241

Protein-protein interaction databases

BioGridi35817. 36 interactions.
DIPiDIP-5065N.
IntActiP53983. 8 interactions.
MINTiMINT-543525.

Structurei

3D structure databases

ProteinModelPortaliP53983.
SMRiP53983. Positions 612-675.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Contains 1 RING-type zinc finger.Curated

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri624 – 66441RING-type; atypicalAdd
BLAST

Keywords - Domaini

Transmembrane, Transmembrane helix, Zinc-finger

Phylogenomic databases

GeneTreeiENSGT00530000069536.
InParanoidiP53983.
OMAiIMIREEN.
OrthoDBiEOG7BZW1Z.

Sequencei

Sequence statusi: Complete.

P53983-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTNILQHVK QLLHNRDVFS FFHNKTGNLN YLDNTTQKPE VFVSPNSTIV
60 70 80 90 100
SAPTLDSFQA LMEKGNFTTL QLAKVGIRMF FSYSVSKYAV LCFSTAIILN
110 120 130 140 150
RLTVMSSLRS NSTNIRLPLW SKTLLHLVAT LSLVKALLQI LSQFGLMHEL
160 170 180 190 200
HVSDTDFYAL SVYLFVALSD CIEIFISSTT NVPSLICSDF SIWGLSLNLY
210 220 230 240 250
IISKMPAGQQ HIGDNVELLG AVFHRLVIHL VELFHIRAYR LCGEVILNAG
260 270 280 290 300
FFTAFVTRTY LNGLDFINIC LIHNYFPGFF YISTILLASI GIFLKALFTS
310 320 330 340 350
NPFRSLYSRY KNLEKWWRSN NYNGEEEFNE IALSLCLLLT SNDYKIFKKS
360 370 380 390 400
DNVKSVDEVA AFSNSYVVSG HLNQLQSTPE DLLSRKEMTT DSQLPGFART
410 420 430 440 450
YLGLFELVRT IILTYSRLLK NLLWSKNFES SIDKKPRVGK RKKRDLNKYV
460 470 480 490 500
TEKNYKKFLY KPDVKELNIE SDLRSLELLL PEDDSSKDYF PPRKIDESVS
510 520 530 540 550
DEEFDSDMES QLIIDEEKEL THLSSNAVDS DDLEEIAWNI SMWSILNYEM
560 570 580 590 600
DVHNKVNGPL TRSQYGKRNP QGVLVDVVIE RLLHHTNSRY MYKRLNMKDD
610 620 630 640 650
DKLEFKFDFA FDSCDEVEEM DLSCLICKVN KRNIVTWPCR CLALCDDCRI
660 670
SLGYKGFATC VSCDSEVKGY SKLNIV
Length:676
Mass (Da):77,649
Last modified:September 21, 2011 - v2
Checksum:i58ECCEA014034A3B
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti1 – 1515MSTNI…QLLHN → MLSNCSIT in CAA95867 (PubMed:9169873).CuratedAdd
BLAST
Sequence conflicti1 – 1515MSTNI…QLLHN → MLSNCSIT in CAA95868 (PubMed:9169873).CuratedAdd
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z71284 Genomic DNA. Translation: CAA95868.1.
Z71283 Genomic DNA. Translation: CAA95867.1.
BK006947 Genomic DNA. Translation: DAA10535.2.
PIRiS62919.
RefSeqiNP_014390.2. NM_001182847.2.

Genome annotation databases

EnsemblFungiiYNL008C; YNL008C; YNL008C.
GeneIDi855724.
KEGGisce:YNL008C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z71284 Genomic DNA. Translation: CAA95868.1.
Z71283 Genomic DNA. Translation: CAA95867.1.
BK006947 Genomic DNA. Translation: DAA10535.2.
PIRiS62919.
RefSeqiNP_014390.2. NM_001182847.2.

3D structure databases

ProteinModelPortaliP53983.
SMRiP53983. Positions 612-675.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi35817. 36 interactions.
DIPiDIP-5065N.
IntActiP53983. 8 interactions.
MINTiMINT-543525.

Proteomic databases

MaxQBiP53983.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYNL008C; YNL008C; YNL008C.
GeneIDi855724.
KEGGisce:YNL008C.

Organism-specific databases

EuPathDBiFungiDB:YNL008C.
SGDiS000004953. ASI3.

Phylogenomic databases

GeneTreeiENSGT00530000069536.
InParanoidiP53983.
OMAiIMIREEN.
OrthoDBiEOG7BZW1Z.

Enzyme and pathway databases

BioCyciYEAST:G3O-33050-MONOMER.

Miscellaneous databases

PROiP53983.

Family and domain databases

ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications."
    Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J.
    , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.
    Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION, SEQUENCE REVISION.
    Strain: ATCC 204508 / S288c.
  3. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  4. "A global topology map of the Saccharomyces cerevisiae membrane proteome."
    Kim H., Melen K., Oesterberg M., von Heijne G.
    Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
    Strain: ATCC 208353 / W303-1A.
  5. "Inner nuclear membrane proteins Asi1, Asi2, and Asi3 function in concert to maintain the latent properties of transcription factors Stp1 and Stp2."
    Zargari A., Boban M., Heessen S., Andreasson C., Thyberg J., Ljungdahl P.O.
    J. Biol. Chem. 282:594-605(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, GLYCOSYLATION, INTERACTION WITH ASI1.

Entry informationi

Entry nameiASI3_YEAST
AccessioniPrimary (citable) accession number: P53983
Secondary accession number(s): D6W1G9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: September 21, 2011
Last modified: June 8, 2016
This is version 102 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XIV
    Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.