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Protein

Isocitrate dehydrogenase [NADP]

Gene

IDP3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

May function in the production of NADPH for fatty acid and sterol synthesis.

Catalytic activityi

Isocitrate + NADP+ = 2-oxoglutarate + CO2 + NADPH.

Cofactori

Mg2+By similarity, Mn2+By similarityNote: Binds 1 Mg2+ or Mn2+ ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei77SubstrateBy similarity1
Binding sitei82NADPBy similarity1
Binding sitei109SubstrateBy similarity1
Binding sitei132SubstrateBy similarity1
Sitei139Critical for catalysisBy similarity1
Sitei212Critical for catalysisBy similarity1
Metal bindingi252Magnesium or manganeseBy similarity1
Binding sitei260NADPBy similarity1
Metal bindingi275Magnesium or manganeseBy similarity1
Binding sitei328NADP; via amide nitrogen and carbonyl oxygenBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi75 – 77NADPBy similarity3
Nucleotide bindingi310 – 315NADPBy similarity6

GO - Molecular functioni

  • isocitrate dehydrogenase (NADP+) activity Source: SGD
  • magnesium ion binding Source: InterPro
  • NAD binding Source: InterPro

GO - Biological processi

  • fatty acid beta-oxidation Source: SGD
  • glyoxylate cycle Source: UniProtKB-KW
  • isocitrate metabolic process Source: InterPro
  • NADPH regeneration Source: SGD
  • tricarboxylic acid cycle Source: UniProtKB-KW

Keywordsi

Molecular functionOxidoreductase
Biological processGlyoxylate bypass, Tricarboxylic acid cycle
LigandMagnesium, Manganese, Metal-binding, NADP

Enzyme and pathway databases

BioCyciYEAST:YNL009W-MONOMER
ReactomeiR-SCE-2151201 Transcriptional activation of mitochondrial biogenesis
R-SCE-389542 NADPH regeneration
R-SCE-6798695 Neutrophil degranulation
R-SCE-71403 Citric acid cycle (TCA cycle)
R-SCE-9033241 Peroxisomal protein import
SABIO-RKiP53982

Names & Taxonomyi

Protein namesi
Recommended name:
Isocitrate dehydrogenase [NADP] (EC:1.1.1.42)
Short name:
IDH
Alternative name(s):
IDP
NADP(+)-specific ICDH
Oxalosuccinate decarboxylase
Gene namesi
Name:IDP3
Ordered Locus Names:YNL009W
ORF Names:N2870
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIV

Organism-specific databases

EuPathDBiFungiDB:YNL009W
SGDiS000004954 IDP3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000835881 – 420Isocitrate dehydrogenase [NADP]Add BLAST420

Proteomic databases

MaxQBiP53982
PaxDbiP53982
PRIDEiP53982

PTM databases

CarbonylDBiP53982

Interactioni

Protein-protein interaction databases

BioGridi35816, 94 interactors
DIPiDIP-4693N
IntActiP53982, 4 interactors
MINTiP53982
STRINGi4932.YNL009W

Structurei

3D structure databases

ProteinModelPortaliP53982
SMRiP53982
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni94 – 100Substrate bindingBy similarity7

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00920000150533
HOGENOMiHOG000019858
InParanoidiP53982
KOiK00031
OMAiIQKRWPL
OrthoDBiEOG092C2D51

Family and domain databases

InterProiView protein in InterPro
IPR019818 IsoCit/isopropylmalate_DH_CS
IPR004790 Isocitrate_DH_NADP
IPR024084 IsoPropMal-DH-like_dom
PANTHERiPTHR11822 PTHR11822, 1 hit
PfamiView protein in Pfam
PF00180 Iso_dh, 1 hit
PIRSFiPIRSF000108 IDH_NADP, 1 hit
SMARTiView protein in SMART
SM01329 Iso_dh, 1 hit
TIGRFAMsiTIGR00127 nadp_idh_euk, 1 hit
PROSITEiView protein in PROSITE
PS00470 IDH_IMDH, 1 hit

Sequencei

Sequence statusi: Complete.

P53982-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSKIKVVHPI VEMDGDEQTR VIWKLIKEKL ILPYLDVDLK YYDLSIQERD
60 70 80 90 100
RTNDQVTKDS SYATLKYGVA VKCATITPDE ARMKEFNLKE MWKSPNGTIR
110 120 130 140 150
NILGGTVFRE PIIIPKIPRL VPHWEKPIII GRHAFGDQYR ATDIKIKKAG
160 170 180 190 200
KLRLQFSSDD GKENIDLKVY EFPKSGGIAM AMFNTNDSIK GFAKASFELA
210 220 230 240 250
LKRKLPLFFT TKNTILKNYD NQFKQIFDNL FDKEYKEKFQ ALKITYEHRL
260 270 280 290 300
IDDMVAQMLK SKGGFIIAMK NYDGDVQSDI VAQGFGSLGL MTSILITPDG
310 320 330 340 350
KTFESEAAHG TVTRHFRKHQ RGEETSTNSI ASIFAWTRAI IQRGKLDNTD
360 370 380 390 400
DVIKFGNLLE KATLDTVQVG GKMTKDLALM LGKTNRSSYV TTEEFIDEVA
410 420
KRLQNMMLSS NEDKKGMCKL
Length:420
Mass (Da):47,856
Last modified:October 1, 1996 - v1
Checksum:i4094007E13FCD506
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z71285 Genomic DNA Translation: CAA95869.1
AY693154 Genomic DNA Translation: AAT93173.1
BK006947 Genomic DNA Translation: DAA10534.1
PIRiS62921
RefSeqiNP_014389.3, NM_001182848.3

Genome annotation databases

EnsemblFungiiYNL009W; YNL009W; YNL009W
GeneIDi855723
KEGGisce:YNL009W

Similar proteinsi

Entry informationi

Entry nameiIDHH_YEAST
AccessioniPrimary (citable) accession number: P53982
Secondary accession number(s): D6W1G8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: June 20, 2018
This is version 148 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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