P53982 (IDHH_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Isocitrate dehydrogenase [NADP]
Short name=IDH
EC=1.1.1.42

Alternative name(s):
IDP
NADP(+)-specific ICDH
Oxalosuccinate decarboxylase

Gene names

Name:	IDP3
Ordered Locus Names:	YNL009W
ORF Names:	N2870

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	420 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May function in the production of NADPH for fatty acid and sterol synthesis.
Catalytic activity	Isocitrate + NADP+ = 2-oxoglutarate + CO2 + NADPH.
Cofactor	Binds 1 magnesium or manganese ion per subunit By similarity.
Sequence similarities	Belongs to the isocitrate and isopropylmalate dehydrogenases family.

Ontologies

Keywords
Biological process	Glyoxylate bypass Tricarboxylic acid cycle
Ligand	Magnesium Manganese Metal-binding NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	NADPH regeneration Traceable author statement PubMed 9452501. Source: SGD fatty acid beta-oxidation Inferred from mutant phenotype PubMed 9452501. Source: SGD glyoxylate cycle Inferred from electronic annotation. Source: UniProtKB-KW isocitrate metabolic process Traceable author statement PubMed 9175438. Source: SGD tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	peroxisome Inferred from direct assay PubMed 9452501. Source: SGD
Molecular_function	NAD binding Inferred from electronic annotation. Source: InterPro isocitrate dehydrogenase (NADP+) activity Traceable author statement PubMed 9175438. Source: SGD magnesium ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 420	420	Isocitrate dehydrogenase [NADP]		PRO_0000083588
Regions
	Nucleotide binding	75 – 77	3	NADP By similarity
	Nucleotide binding	310 – 315	6	NADP By similarity
	Region	94 – 100	7	Substrate binding By similarity
Sites
	Metal binding	252	1	Magnesium or manganese By similarity
	Metal binding	275	1	Magnesium or manganese By similarity
	Binding site	77	1	Substrate By similarity
	Binding site	82	1	NADP By similarity
	Binding site	109	1	Substrate By similarity
	Binding site	132	1	Substrate By similarity
	Binding site	260	1	NADP By similarity
	Binding site	328	1	NADP; via amide nitrogen and carbonyl oxygen By similarity
	Site	139	1	Critical for catalysis By similarity
	Site	212	1	Critical for catalysis By similarity

Sequences

	Sequence		Length	Mass (Da)
	P53982 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 4094007E13FCD506	FASTA	420	47,856
	10 20 30 40 50 60 MSKIKVVHPI VEMDGDEQTR VIWKLIKEKL ILPYLDVDLK YYDLSIQERD RTNDQVTKDS 70 80 90 100 110 120 SYATLKYGVA VKCATITPDE ARMKEFNLKE MWKSPNGTIR NILGGTVFRE PIIIPKIPRL 130 140 150 160 170 180 VPHWEKPIII GRHAFGDQYR ATDIKIKKAG KLRLQFSSDD GKENIDLKVY EFPKSGGIAM 190 200 210 220 230 240 AMFNTNDSIK GFAKASFELA LKRKLPLFFT TKNTILKNYD NQFKQIFDNL FDKEYKEKFQ 250 260 270 280 290 300 ALKITYEHRL IDDMVAQMLK SKGGFIIAMK NYDGDVQSDI VAQGFGSLGL MTSILITPDG 310 320 330 340 350 360 KTFESEAAHG TVTRHFRKHQ RGEETSTNSI ASIFAWTRAI IQRGKLDNTD DVIKFGNLLE 370 380 390 400 410 420 KATLDTVQVG GKMTKDLALM LGKTNRSSYV TTEEFIDEVA KRLQNMMLSS NEDKKGMCKL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications." Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J. , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J. Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z71285 Genomic DNA. Translation: CAA95869.1. AY693154 Genomic DNA. Translation: AAT93173.1. BK006947 Genomic DNA. Translation: DAA10534.1.
PIR	S62921.
RefSeq	NP_014389.3. NM_001182848.3.
3D structure databases
ProteinModelPortal	P53982.
SMR	P53982. Positions 2-404.
ModBase	Search...
MobiDB	Search...
Protein-protein interaction databases
BioGrid	35816. 30 interactions.
DIP	DIP-4693N.
IntAct	P53982. 4 interactions.
MINT	MINT-473180.
STRING	4932.YNL009W.
Proteomic databases
PaxDb	P53982.
PeptideAtlas	P53982.
PRIDE	P53982.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YNL009W; YNL009W; YNL009W.
GeneID	855723.
KEGG	sce:YNL009W.
Organism-specific databases
CYGD	YNL009w.
SGD	S000004954. IDP3.
Phylogenomic databases
eggNOG	COG0538.
GeneTree	ENSGT00740000116441.
HOGENOM	HOG000019858.
KO	K00031.
OMA	PHWEKPI.
OrthoDB	EOG7G4QQD.
Enzyme and pathway databases
BioCyc	YEAST:YNL009W-MONOMER.
SABIO-RK	P53982.
Gene expression databases
Genevestigator	P53982.
Family and domain databases
Gene3D	3.40.718.10. 1 hit.
InterPro	IPR019818. IsoCit/isopropylmalate_DH_CS. IPR004790. Isocitrate_DH_NADP. IPR024084. IsoPropMal-DH-like_dom. [Graphical view]
PANTHER	PTHR11822. PTHR11822. 1 hit.
Pfam	PF00180. Iso_dh. 1 hit. [Graphical view]
PIRSF	PIRSF000108. IDH_NADP. 1 hit.
TIGRFAMs	TIGR00127. nadp_idh_euk. 1 hit.
PROSITE	PS00470. IDH_IMDH. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	980090.

Entry information

Entry name

IDHH_YEAST

Accession

Primary (citable) accession number: P53982
Secondary accession number(s): D6W1G8

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	February 19, 2014
This is version 111 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program