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P53982

- IDHH_YEAST

UniProt

P53982 - IDHH_YEAST

Protein

Isocitrate dehydrogenase [NADP]

Gene

IDP3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 116 (01 Oct 2014)
      Sequence version 1 (01 Oct 1996)
      Previous versions | rss
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    Functioni

    May function in the production of NADPH for fatty acid and sterol synthesis.

    Catalytic activityi

    Isocitrate + NADP+ = 2-oxoglutarate + CO2 + NADPH.

    Cofactori

    Binds 1 magnesium or manganese ion per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei77 – 771SubstrateBy similarity
    Binding sitei82 – 821NADPBy similarity
    Binding sitei109 – 1091SubstrateBy similarity
    Binding sitei132 – 1321SubstrateBy similarity
    Sitei139 – 1391Critical for catalysisBy similarity
    Sitei212 – 2121Critical for catalysisBy similarity
    Metal bindingi252 – 2521Magnesium or manganeseBy similarity
    Binding sitei260 – 2601NADPBy similarity
    Metal bindingi275 – 2751Magnesium or manganeseBy similarity
    Binding sitei328 – 3281NADP; via amide nitrogen and carbonyl oxygenBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi75 – 773NADPBy similarity
    Nucleotide bindingi310 – 3156NADPBy similarity

    GO - Molecular functioni

    1. isocitrate dehydrogenase (NADP+) activity Source: SGD
    2. magnesium ion binding Source: InterPro
    3. NAD binding Source: InterPro

    GO - Biological processi

    1. fatty acid beta-oxidation Source: SGD
    2. glyoxylate cycle Source: UniProtKB-KW
    3. isocitrate metabolic process Source: InterPro
    4. NADPH regeneration Source: SGD
    5. tricarboxylic acid cycle Source: UniProtKB-KW

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Glyoxylate bypass, Tricarboxylic acid cycle

    Keywords - Ligandi

    Magnesium, Manganese, Metal-binding, NADP

    Enzyme and pathway databases

    BioCyciYEAST:YNL009W-MONOMER.
    ReactomeiREACT_207320. Abnormal conversion of 2-oxoglutarate to 2-hydroxyglutarate.
    REACT_227890. NADPH regeneration.
    SABIO-RKP53982.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isocitrate dehydrogenase [NADP] (EC:1.1.1.42)
    Short name:
    IDH
    Alternative name(s):
    IDP
    NADP(+)-specific ICDH
    Oxalosuccinate decarboxylase
    Gene namesi
    Name:IDP3
    Ordered Locus Names:YNL009W
    ORF Names:N2870
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XIV

    Organism-specific databases

    CYGDiYNL009w.
    SGDiS000004954. IDP3.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: SGD
    2. peroxisome Source: SGD

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 420420Isocitrate dehydrogenase [NADP]PRO_0000083588Add
    BLAST

    Proteomic databases

    MaxQBiP53982.
    PaxDbiP53982.
    PeptideAtlasiP53982.
    PRIDEiP53982.

    Expressioni

    Gene expression databases

    GenevestigatoriP53982.

    Interactioni

    Protein-protein interaction databases

    BioGridi35816. 30 interactions.
    DIPiDIP-4693N.
    IntActiP53982. 4 interactions.
    MINTiMINT-473180.
    STRINGi4932.YNL009W.

    Structurei

    3D structure databases

    ProteinModelPortaliP53982.
    SMRiP53982. Positions 2-404.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni94 – 1007Substrate bindingBy similarity

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0538.
    GeneTreeiENSGT00390000012547.
    HOGENOMiHOG000019858.
    KOiK00031.
    OMAiDESIWGF.
    OrthoDBiEOG7G4QQD.

    Family and domain databases

    Gene3Di3.40.718.10. 1 hit.
    InterProiIPR019818. IsoCit/isopropylmalate_DH_CS.
    IPR004790. Isocitrate_DH_NADP.
    IPR024084. IsoPropMal-DH-like_dom.
    [Graphical view]
    PANTHERiPTHR11822. PTHR11822. 1 hit.
    PfamiPF00180. Iso_dh. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000108. IDH_NADP. 1 hit.
    TIGRFAMsiTIGR00127. nadp_idh_euk. 1 hit.
    PROSITEiPS00470. IDH_IMDH. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P53982-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSKIKVVHPI VEMDGDEQTR VIWKLIKEKL ILPYLDVDLK YYDLSIQERD    50
    RTNDQVTKDS SYATLKYGVA VKCATITPDE ARMKEFNLKE MWKSPNGTIR 100
    NILGGTVFRE PIIIPKIPRL VPHWEKPIII GRHAFGDQYR ATDIKIKKAG 150
    KLRLQFSSDD GKENIDLKVY EFPKSGGIAM AMFNTNDSIK GFAKASFELA 200
    LKRKLPLFFT TKNTILKNYD NQFKQIFDNL FDKEYKEKFQ ALKITYEHRL 250
    IDDMVAQMLK SKGGFIIAMK NYDGDVQSDI VAQGFGSLGL MTSILITPDG 300
    KTFESEAAHG TVTRHFRKHQ RGEETSTNSI ASIFAWTRAI IQRGKLDNTD 350
    DVIKFGNLLE KATLDTVQVG GKMTKDLALM LGKTNRSSYV TTEEFIDEVA 400
    KRLQNMMLSS NEDKKGMCKL 420
    Length:420
    Mass (Da):47,856
    Last modified:October 1, 1996 - v1
    Checksum:i4094007E13FCD506
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z71285 Genomic DNA. Translation: CAA95869.1.
    AY693154 Genomic DNA. Translation: AAT93173.1.
    BK006947 Genomic DNA. Translation: DAA10534.1.
    PIRiS62921.
    RefSeqiNP_014389.3. NM_001182848.3.

    Genome annotation databases

    EnsemblFungiiYNL009W; YNL009W; YNL009W.
    GeneIDi855723.
    KEGGisce:YNL009W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z71285 Genomic DNA. Translation: CAA95869.1 .
    AY693154 Genomic DNA. Translation: AAT93173.1 .
    BK006947 Genomic DNA. Translation: DAA10534.1 .
    PIRi S62921.
    RefSeqi NP_014389.3. NM_001182848.3.

    3D structure databases

    ProteinModelPortali P53982.
    SMRi P53982. Positions 2-404.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 35816. 30 interactions.
    DIPi DIP-4693N.
    IntActi P53982. 4 interactions.
    MINTi MINT-473180.
    STRINGi 4932.YNL009W.

    Proteomic databases

    MaxQBi P53982.
    PaxDbi P53982.
    PeptideAtlasi P53982.
    PRIDEi P53982.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YNL009W ; YNL009W ; YNL009W .
    GeneIDi 855723.
    KEGGi sce:YNL009W.

    Organism-specific databases

    CYGDi YNL009w.
    SGDi S000004954. IDP3.

    Phylogenomic databases

    eggNOGi COG0538.
    GeneTreei ENSGT00390000012547.
    HOGENOMi HOG000019858.
    KOi K00031.
    OMAi DESIWGF.
    OrthoDBi EOG7G4QQD.

    Enzyme and pathway databases

    BioCyci YEAST:YNL009W-MONOMER.
    Reactomei REACT_207320. Abnormal conversion of 2-oxoglutarate to 2-hydroxyglutarate.
    REACT_227890. NADPH regeneration.
    SABIO-RK P53982.

    Miscellaneous databases

    NextBioi 980090.

    Gene expression databases

    Genevestigatori P53982.

    Family and domain databases

    Gene3Di 3.40.718.10. 1 hit.
    InterProi IPR019818. IsoCit/isopropylmalate_DH_CS.
    IPR004790. Isocitrate_DH_NADP.
    IPR024084. IsoPropMal-DH-like_dom.
    [Graphical view ]
    PANTHERi PTHR11822. PTHR11822. 1 hit.
    Pfami PF00180. Iso_dh. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000108. IDH_NADP. 1 hit.
    TIGRFAMsi TIGR00127. nadp_idh_euk. 1 hit.
    PROSITEi PS00470. IDH_IMDH. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications."
      Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J.
      , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.
      Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    3. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.

    Entry informationi

    Entry nameiIDHH_YEAST
    AccessioniPrimary (citable) accession number: P53982
    Secondary accession number(s): D6W1G8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 116 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome XIV
      Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

    External Data

    Dasty 3