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Protein

Elongation factor 3B

Gene

HEF3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Weakly expressed functional homolog of translation elongation factor 3A (YEF3), not required for survival. Can complement loss of YEF3 when expressed from the YEF3 or ADH1 promoters. Required for the ATP-dependent release of deacylated tRNA from the ribosomal E-site during protein biosynthesis. Stimulates the eEF1A-dependent binding of aminoacyl-tRNA to the ribosomal A-site, which has reduced affinity for tRNA as long as the E-site is occupied.

Kineticsi

  1. KM=0.102 mM for ATP1 Publication
  1. Vmax=5.7 µmol/min/mg enzyme with ATP as substrate1 Publication

Pathwayi: polypeptide chain elongation

This protein is involved in the pathway polypeptide chain elongation, which is part of Protein biosynthesis.
View all proteins of this organism that are known to be involved in the pathway polypeptide chain elongation and in Protein biosynthesis.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi463 – 470ATP 1PROSITE-ProRule annotation8
Nucleotide bindingi701 – 708ATP 2PROSITE-ProRule annotation8

GO - Molecular functioni

  • ATPase activity Source: SGD
  • ATP binding Source: UniProtKB-KW
  • translation elongation factor activity Source: SGD

GO - Biological processi

  • translational elongation Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Elongation factor

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding, RNA-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-33053-MONOMER.
UniPathwayiUPA00345.

Names & Taxonomyi

Protein namesi
Recommended name:
Elongation factor 3B
Short name:
EF-3B
Alternative name(s):
Homolog of EF-3
Translation elongation factor 3B
Gene namesi
Name:HEF3
Synonyms:YEF3B, ZRG7
Ordered Locus Names:YNL014W
ORF Names:N2846
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIV

Organism-specific databases

EuPathDBiFungiDB:YNL014W.
SGDiS000004959. HEF3.

Subcellular locationi

GO - Cellular componenti

  • cytosolic ribosome Source: SGD
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00000934592 – 1044Elongation factor 3BAdd BLAST1043

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineBy similarity1
Modified residuei187N6,N6,N6-trimethyllysineBy similarity1
Modified residuei196N6,N6,N6-trimethyllysineBy similarity1
Modified residuei789N6,N6,N6-trimethyllysineBy similarity1
Modified residuei972PhosphothreonineBy similarity1
Modified residuei974PhosphoserineBy similarity1
Modified residuei1039PhosphoserineBy similarity1
Modified residuei1040PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Methylation, Phosphoprotein

Proteomic databases

MaxQBiP53978.
PRIDEiP53978.

PTM databases

iPTMnetiP53978.

Expressioni

Developmental stagei

Expressed in vegetatively growing cells at very low levels.2 Publications

Interactioni

Protein-protein interaction databases

BioGridi35812. 32 interactors.
DIPiDIP-6529N.
IntActiP53978. 8 interactors.
MINTiMINT-704227.

Structurei

3D structure databases

ProteinModelPortaliP53978.
SMRiP53978.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati5 – 42HEAT 1Add BLAST38
Repeati86 – 123HEAT 2Add BLAST38
Repeati124 – 162HEAT 3Add BLAST39
Repeati166 – 203HEAT 4Add BLAST38
Repeati205 – 241HEAT 5Add BLAST37
Repeati242 – 279HEAT 6Add BLAST38
Repeati285 – 323HEAT 7Add BLAST39
Domaini426 – 641ABC transporter 1PROSITE-ProRule annotationAdd BLAST216
Domaini667 – 993ABC transporter 2PROSITE-ProRule annotationAdd BLAST327

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi1009 – 1031Lys-rich (basic)Add BLAST23

Sequence similaritiesi

Contains 2 ABC transporter domains.PROSITE-ProRule annotation
Contains 7 HEAT repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

GeneTreeiENSGT00550000075671.
HOGENOMiHOG000180957.
InParanoidiP53978.
KOiK03235.
OMAiAYIANET.
OrthoDBiEOG092C22OB.

Family and domain databases

Gene3Di1.25.10.10. 1 hit.
3.40.50.300. 3 hits.
InterProiIPR003593. AAA+_ATPase.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR015688. Elongation_fac_3.
IPR021133. HEAT_type_2.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR19211:SF5. PTHR19211:SF5. 2 hits.
PfamiPF00005. ABC_tran. 2 hits.
[Graphical view]
SMARTiSM00382. AAA. 2 hits.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 7 hits.
SSF52540. SSF52540. 3 hits.
PROSITEiPS00211. ABC_TRANSPORTER_1. 2 hits.
PS50893. ABC_TRANSPORTER_2. 2 hits.
PS50077. HEAT_REPEAT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P53978-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSDSQQSITV LEELFRKLET ATSETREGIS SELSSFLNGN IIEHDVPEVF
60 70 80 90 100
FDEFQKAIQS KQKALNTLGA VAYIANETNL SPSVEPYIVA TVPSVCSKAG
110 120 130 140 150
SKDNDVQLAA TKALKAIASA VNPVAVKALL PHLIHSLETS NKWKEKVAVL
160 170 180 190 200
EVISVLVDAA KEQIALRMPE LIPVLSESMW DTKKGVKEAA TTTITKATET
210 220 230 240 250
VDNKDIERFI PKLIECIANP NEVPETVHLL GATTFVAEVT PATLSIMVPL
260 270 280 290 300
LSRGLAERET SIKRKAAVII DNMCKLVEDP QVVAPFLGKL LPGLKNNFAT
310 320 330 340 350
IADPEAREVT LKALKTLRRV GNVGEDDVLP EISHAGDVST TLGVIKELLE
360 370 380 390 400
PEKVAPRFTI VVEYIAAIAA NLIDERIIDQ QTWFTHVTPY MTIFLHEKTA
410 420 430 440 450
KEILDDFRKR AVDNIPVGPN FQDEEDEGED LCNCEFSLAY GAKILLNKTQ
460 470 480 490 500
LRLKRGRRYG LCGPNGAGKS TLMRSIANGQ VDGFPTQDEC RTVYVEHDID
510 520 530 540 550
NTHSDMSVLD FVYSGNVGTK DVITSKLKEF GFSDEMIEMP IASLSGGWKM
560 570 580 590 600
KLALARAVLK DADILLLDEP TNHLDTVNVE WLVNYLNTCG ITSVIVSHDS
610 620 630 640 650
GFLDKVCQYI IHYEGLKLRK YKGNLSEFVQ KCPTAQSYYE LGASDLEFQF
660 670 680 690 700
PTPGYLEGVK TKQKAIVKVS NMTFQYPGTT KPQVSDVTFQ CSLSSRIAVI
710 720 730 740 750
GPNGAGKSTL INVLTGELLP TSGEVYTHEN CRIAYIKQHA FAHIESHLDK
760 770 780 790 800
TPSEYIQWRF QTGEDRETMD RANRQINEND AEAMNKIFKI EGTPRRVAGI
810 820 830 840 850
HSRRKFKNTY EYECSFLLGE NIGMKSERWV PMMSVDNAWL PRGELIESHS
860 870 880 890 900
KMVAEIDMKE ALASGQFRAL TRKEIELHCA MLGLDSELVS HSRIRGLSGG
910 920 930 940 950
QKVKLVLAAC TWQRPHLIVL DEPTNYLDRD SLGALSKALK AFEGGVIIIT
960 970 980 990 1000
HSAEFTKNLT DEVWAVKDGK MTPSGHNWVA GQGAGPRIEK KEEEGDKFDA
1010 1020 1030 1040
MGNKINSGKK KSKLSSAELR KKKKERMKKK KEMGDEYVSS DEDF
Length:1,044
Mass (Da):115,869
Last modified:January 23, 2007 - v2
Checksum:i7E040EE826D550F1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z71290 Genomic DNA. Translation: CAA95874.1.
BK006947 Genomic DNA. Translation: DAA10530.1.
PIRiS62926.
RefSeqiNP_014384.3. NM_001182853.3.

Genome annotation databases

EnsemblFungiiYNL014W; YNL014W; YNL014W.
GeneIDi855718.
KEGGisce:YNL014W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z71290 Genomic DNA. Translation: CAA95874.1.
BK006947 Genomic DNA. Translation: DAA10530.1.
PIRiS62926.
RefSeqiNP_014384.3. NM_001182853.3.

3D structure databases

ProteinModelPortaliP53978.
SMRiP53978.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi35812. 32 interactors.
DIPiDIP-6529N.
IntActiP53978. 8 interactors.
MINTiMINT-704227.

PTM databases

iPTMnetiP53978.

Proteomic databases

MaxQBiP53978.
PRIDEiP53978.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYNL014W; YNL014W; YNL014W.
GeneIDi855718.
KEGGisce:YNL014W.

Organism-specific databases

EuPathDBiFungiDB:YNL014W.
SGDiS000004959. HEF3.

Phylogenomic databases

GeneTreeiENSGT00550000075671.
HOGENOMiHOG000180957.
InParanoidiP53978.
KOiK03235.
OMAiAYIANET.
OrthoDBiEOG092C22OB.

Enzyme and pathway databases

UniPathwayiUPA00345.
BioCyciYEAST:G3O-33053-MONOMER.

Miscellaneous databases

PROiP53978.

Family and domain databases

Gene3Di1.25.10.10. 1 hit.
3.40.50.300. 3 hits.
InterProiIPR003593. AAA+_ATPase.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR015688. Elongation_fac_3.
IPR021133. HEAT_type_2.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR19211:SF5. PTHR19211:SF5. 2 hits.
PfamiPF00005. ABC_tran. 2 hits.
[Graphical view]
SMARTiSM00382. AAA. 2 hits.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 7 hits.
SSF52540. SSF52540. 3 hits.
PROSITEiPS00211. ABC_TRANSPORTER_1. 2 hits.
PS50893. ABC_TRANSPORTER_2. 2 hits.
PS50077. HEAT_REPEAT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiEF3B_YEAST
AccessioniPrimary (citable) accession number: P53978
Secondary accession number(s): D6W1G4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: January 23, 2007
Last modified: November 2, 2016
This is version 151 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 125 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XIV
    Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.