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P53974

- ARK1_YEAST

UniProt

P53974 - ARK1_YEAST

Protein

Actin-regulating kinase 1

Gene

ARK1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 125 (01 Oct 2014)
      Sequence version 1 (01 Oct 1996)
      Previous versions | rss
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    Functioni

    Involved in regulation of actin cytoskeleton organization and endocytosis.2 Publications

    Catalytic activityi

    ATP + a protein = ADP + a phosphoprotein.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei56 – 561ATPPROSITE-ProRule annotation
    Active sitei159 – 1591Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi28 – 369ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. protein binding Source: IntAct
    3. protein serine/threonine kinase activity Source: SGD

    GO - Biological processi

    1. actin cortical patch assembly Source: SGD
    2. actin filament organization Source: SGD
    3. protein phosphorylation Source: SGD
    4. regulation of endocytosis Source: SGD

    Keywords - Molecular functioni

    Kinase, Serine/threonine-protein kinase, Transferase

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciYEAST:G3O-33058-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Actin-regulating kinase 1 (EC:2.7.11.1)
    Gene namesi
    Name:ARK1
    Ordered Locus Names:YNL020C
    ORF Names:N2823
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XIV

    Organism-specific databases

    CYGDiYNL020c.
    SGDiS000004965. ARK1.

    Subcellular locationi

    Cytoplasmcytoskeletonactin patch 2 Publications
    Note: Cortical actin patches.

    GO - Cellular componenti

    1. actin cortical patch Source: SGD

    Keywords - Cellular componenti

    Cytoplasm, Cytoskeleton

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi56 – 561K → A: Abolishes protein kinase activity. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 638638Actin-regulating kinase 1PRO_0000085635Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei478 – 4781Phosphoserine2 Publications
    Modified residuei522 – 5221Phosphoserine1 Publication
    Modified residuei535 – 5351Phosphoserine2 Publications

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP53974.
    PaxDbiP53974.

    Expressioni

    Gene expression databases

    GenevestigatoriP53974.

    Interactioni

    Subunit structurei

    Interacts with ABP1, which is required for proper actin patch localization.1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    ABP1P1589111EBI-9817,EBI-2036

    Protein-protein interaction databases

    BioGridi35806. 68 interactions.
    DIPiDIP-4381N.
    IntActiP53974. 18 interactions.
    MINTiMINT-511765.
    STRINGi4932.YNL020C.

    Structurei

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2RPNNMR-B605-621[»]
    ProteinModelPortaliP53974.
    SMRiP53974. Positions 11-338.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP53974.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini22 – 298277Protein kinasePROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni602 – 61514Interaction with SH3 domain of ABP1Add
    BLAST

    Sequence similaritiesi

    Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.PROSITE-ProRule annotation
    Contains 1 protein kinase domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0515.
    GeneTreeiENSGT00510000046552.
    HOGENOMiHOG000247884.
    KOiK08853.
    OMAiSANNEYK.
    OrthoDBiEOG74BK1F.

    Family and domain databases

    InterProiIPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view]
    PfamiPF00069. Pkinase. 1 hit.
    [Graphical view]
    SUPFAMiSSF56112. SSF56112. 1 hit.
    PROSITEiPS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P53974-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNQPQIGTYN VGTQLTVGSH QVEIIKYLTS GGFAQVYSAL INPPDPHSNS    50
    SVACLKRVIV PDKPSLNTLR AEVDAMRLLK NNRYVVSYID SHAAKAMLHN 100
    GSYEVFVLME YCERGGLIDF MNTRLQNRLH EFEILQIMSQ VTQGVAAMHA 150
    LQPPLIHRDI KIENVLISAN NEYKLCDFGS VCGIIRPPRN SQELSYVQQD 200
    ILKNTTAQYR SPEMIDTFRG LPIDEKSDIW ALGIFLYKLC YYTTPFEKGG 250
    DLAILSGKFE FPLYPNYSEQ LKGLIRDILV QDPRHRPNVY QLLKRISIMQ 300
    NVPCPINDIQ VVQAPSSHLN LTELHQLSAT QNILSLNSPT TMENTMPNAT 350
    FQISMADNTT TAQMHPNRKP SQIAYDASFS NSAKGSQPLF DKSQNMYHAL 400
    DPPLVEPLAS SVSNNDNELK ANSATKLKQA IVSEAHTFRQ NNSIDFPLQN 450
    IIPQYEDSSS SSDESYSGDV DELKKTRSLG SYSTRGNIKK NQSVKESLTS 500
    SSLPGTSFTP TSTKVNLKHE NSPFKSTFVN TIDNSKDDLN KPSYEDLDVS 550
    KQNLKNSIQQ RMIDKLNSSE ESFNARKMSK VKLHEKGEID KPTMLKSSGP 600
    ISKDKKTKPT PPPKPSHLKP KPPPKPLLLA GRKLSLDK 638
    Length:638
    Mass (Da):71,373
    Last modified:October 1, 1996 - v1
    Checksum:i139CA4CA1B6A181F
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z71296 Genomic DNA. Translation: CAA95882.1.
    BK006947 Genomic DNA. Translation: DAA10525.1.
    PIRiS62932.
    RefSeqiNP_014378.1. NM_001182859.1.

    Genome annotation databases

    EnsemblFungiiYNL020C; YNL020C; YNL020C.
    GeneIDi855711.
    KEGGisce:YNL020C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z71296 Genomic DNA. Translation: CAA95882.1 .
    BK006947 Genomic DNA. Translation: DAA10525.1 .
    PIRi S62932.
    RefSeqi NP_014378.1. NM_001182859.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2RPN NMR - B 605-621 [» ]
    ProteinModelPortali P53974.
    SMRi P53974. Positions 11-338.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 35806. 68 interactions.
    DIPi DIP-4381N.
    IntActi P53974. 18 interactions.
    MINTi MINT-511765.
    STRINGi 4932.YNL020C.

    Proteomic databases

    MaxQBi P53974.
    PaxDbi P53974.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YNL020C ; YNL020C ; YNL020C .
    GeneIDi 855711.
    KEGGi sce:YNL020C.

    Organism-specific databases

    CYGDi YNL020c.
    SGDi S000004965. ARK1.

    Phylogenomic databases

    eggNOGi COG0515.
    GeneTreei ENSGT00510000046552.
    HOGENOMi HOG000247884.
    KOi K08853.
    OMAi SANNEYK.
    OrthoDBi EOG74BK1F.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-33058-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei P53974.
    NextBioi 980061.

    Gene expression databases

    Genevestigatori P53974.

    Family and domain databases

    InterProi IPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view ]
    Pfami PF00069. Pkinase. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56112. SSF56112. 1 hit.
    PROSITEi PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications."
      Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J.
      , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.
      Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    3. "Novel protein kinases Ark1p and Prk1p associate with and regulate the cortical actin cytoskeleton in budding yeast."
      Cope M.J.T.V., Yang S., Shang C., Drubin D.G.
      J. Cell Biol. 144:1203-1218(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION, MUTAGENESIS OF LYS-56.
    4. "In vivo role for actin-regulating kinases in endocytosis and yeast epsin phosphorylation."
      Watson H.A., Cope M.J.T.V., Groen A.C., Drubin D.G., Wendland B.
      Mol. Biol. Cell 12:3668-3679(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    5. "Unusual binding properties of the SH3 domain of the yeast actin-binding protein Abp1: structural and functional analysis."
      Fazi B., Cope M.J.T.V., Douangamath A., Ferracuti S., Schirwitz K., Zucconi A., Drubin D.G., Wilmanns M., Cesareni G., Castagnoli L.
      J. Biol. Chem. 277:5290-5298(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH ABP1.
    6. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    8. "Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
      Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
      Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-478, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    9. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-478; SER-522 AND SER-535, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    10. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-535, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiARK1_YEAST
    AccessioniPrimary (citable) accession number: P53974
    Secondary accession number(s): D6W1F9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 125 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 1551 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome XIV
      Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

    External Data

    Dasty 3