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P53974

- ARK1_YEAST

UniProt

P53974 - ARK1_YEAST

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Protein

Actin-regulating kinase 1

Gene
ARK1, YNL020C, N2823
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Involved in regulation of actin cytoskeleton organization and endocytosis.2 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei56 – 561ATP By similarity
Active sitei159 – 1591Proton acceptor By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi28 – 369ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. protein binding Source: IntAct
  3. protein serine/threonine kinase activity Source: SGD

GO - Biological processi

  1. actin cortical patch assembly Source: SGD
  2. actin filament organization Source: SGD
  3. protein phosphorylation Source: SGD
  4. regulation of endocytosis Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-33058-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Actin-regulating kinase 1 (EC:2.7.11.1)
Gene namesi
Name:ARK1
Ordered Locus Names:YNL020C
ORF Names:N2823
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XIV

Organism-specific databases

CYGDiYNL020c.
SGDiS000004965. ARK1.

Subcellular locationi

Cytoplasmcytoskeletonactin patch
Note: Cortical actin patches.2 Publications

GO - Cellular componenti

  1. actin cortical patch Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi56 – 561K → A: Abolishes protein kinase activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 638638Actin-regulating kinase 1PRO_0000085635Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei478 – 4781Phosphoserine2 Publications
Modified residuei522 – 5221Phosphoserine1 Publication
Modified residuei535 – 5351Phosphoserine2 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP53974.
PaxDbiP53974.

Expressioni

Gene expression databases

GenevestigatoriP53974.

Interactioni

Subunit structurei

Interacts with ABP1, which is required for proper actin patch localization.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
ABP1P1589111EBI-9817,EBI-2036

Protein-protein interaction databases

BioGridi35806. 68 interactions.
DIPiDIP-4381N.
IntActiP53974. 18 interactions.
MINTiMINT-511765.
STRINGi4932.YNL020C.

Structurei

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2RPNNMR-B605-621[»]
ProteinModelPortaliP53974.
SMRiP53974. Positions 11-338.

Miscellaneous databases

EvolutionaryTraceiP53974.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini22 – 298277Protein kinaseAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni602 – 61514Interaction with SH3 domain of ABP1Add
BLAST

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0515.
GeneTreeiENSGT00510000046552.
HOGENOMiHOG000247884.
KOiK08853.
OMAiSANNEYK.
OrthoDBiEOG74BK1F.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P53974-1 [UniParc]FASTAAdd to Basket

« Hide

MNQPQIGTYN VGTQLTVGSH QVEIIKYLTS GGFAQVYSAL INPPDPHSNS    50
SVACLKRVIV PDKPSLNTLR AEVDAMRLLK NNRYVVSYID SHAAKAMLHN 100
GSYEVFVLME YCERGGLIDF MNTRLQNRLH EFEILQIMSQ VTQGVAAMHA 150
LQPPLIHRDI KIENVLISAN NEYKLCDFGS VCGIIRPPRN SQELSYVQQD 200
ILKNTTAQYR SPEMIDTFRG LPIDEKSDIW ALGIFLYKLC YYTTPFEKGG 250
DLAILSGKFE FPLYPNYSEQ LKGLIRDILV QDPRHRPNVY QLLKRISIMQ 300
NVPCPINDIQ VVQAPSSHLN LTELHQLSAT QNILSLNSPT TMENTMPNAT 350
FQISMADNTT TAQMHPNRKP SQIAYDASFS NSAKGSQPLF DKSQNMYHAL 400
DPPLVEPLAS SVSNNDNELK ANSATKLKQA IVSEAHTFRQ NNSIDFPLQN 450
IIPQYEDSSS SSDESYSGDV DELKKTRSLG SYSTRGNIKK NQSVKESLTS 500
SSLPGTSFTP TSTKVNLKHE NSPFKSTFVN TIDNSKDDLN KPSYEDLDVS 550
KQNLKNSIQQ RMIDKLNSSE ESFNARKMSK VKLHEKGEID KPTMLKSSGP 600
ISKDKKTKPT PPPKPSHLKP KPPPKPLLLA GRKLSLDK 638
Length:638
Mass (Da):71,373
Last modified:October 1, 1996 - v1
Checksum:i139CA4CA1B6A181F
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z71296 Genomic DNA. Translation: CAA95882.1.
BK006947 Genomic DNA. Translation: DAA10525.1.
PIRiS62932.
RefSeqiNP_014378.1. NM_001182859.1.

Genome annotation databases

EnsemblFungiiYNL020C; YNL020C; YNL020C.
GeneIDi855711.
KEGGisce:YNL020C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z71296 Genomic DNA. Translation: CAA95882.1 .
BK006947 Genomic DNA. Translation: DAA10525.1 .
PIRi S62932.
RefSeqi NP_014378.1. NM_001182859.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2RPN NMR - B 605-621 [» ]
ProteinModelPortali P53974.
SMRi P53974. Positions 11-338.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 35806. 68 interactions.
DIPi DIP-4381N.
IntActi P53974. 18 interactions.
MINTi MINT-511765.
STRINGi 4932.YNL020C.

Proteomic databases

MaxQBi P53974.
PaxDbi P53974.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YNL020C ; YNL020C ; YNL020C .
GeneIDi 855711.
KEGGi sce:YNL020C.

Organism-specific databases

CYGDi YNL020c.
SGDi S000004965. ARK1.

Phylogenomic databases

eggNOGi COG0515.
GeneTreei ENSGT00510000046552.
HOGENOMi HOG000247884.
KOi K08853.
OMAi SANNEYK.
OrthoDBi EOG74BK1F.

Enzyme and pathway databases

BioCyci YEAST:G3O-33058-MONOMER.

Miscellaneous databases

EvolutionaryTracei P53974.
NextBioi 980061.

Gene expression databases

Genevestigatori P53974.

Family and domain databases

InterProi IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view ]
Pfami PF00069. Pkinase. 1 hit.
[Graphical view ]
SUPFAMi SSF56112. SSF56112. 1 hit.
PROSITEi PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications."
    Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J.
    , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.
    Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. "Novel protein kinases Ark1p and Prk1p associate with and regulate the cortical actin cytoskeleton in budding yeast."
    Cope M.J.T.V., Yang S., Shang C., Drubin D.G.
    J. Cell Biol. 144:1203-1218(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, MUTAGENESIS OF LYS-56.
  4. "In vivo role for actin-regulating kinases in endocytosis and yeast epsin phosphorylation."
    Watson H.A., Cope M.J.T.V., Groen A.C., Drubin D.G., Wendland B.
    Mol. Biol. Cell 12:3668-3679(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  5. "Unusual binding properties of the SH3 domain of the yeast actin-binding protein Abp1: structural and functional analysis."
    Fazi B., Cope M.J.T.V., Douangamath A., Ferracuti S., Schirwitz K., Zucconi A., Drubin D.G., Wilmanns M., Cesareni G., Castagnoli L.
    J. Biol. Chem. 277:5290-5298(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH ABP1.
  6. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  8. "Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
    Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
    Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-478, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  9. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-478; SER-522 AND SER-535, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  10. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-535, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiARK1_YEAST
AccessioniPrimary (citable) accession number: P53974
Secondary accession number(s): D6W1F9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: July 9, 2014
This is version 124 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1551 molecules/cell in log phase SD medium.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XIV
    Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

External Data

Dasty 3

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