P53974 (ARK1_YEAST) Reviewed, UniProtKB/Swiss-Prot
Last modified
December 14, 2011.
Version 99.
History...
Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: Actin-regulating kinase 1 EC=2.7.11.1 | ||||||
| Gene names |
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| Organism | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | ||||||
| Taxonomic identifier | 559292 [NCBI] | ||||||
| Taxonomic lineage | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces |
Protein attributes
| Sequence length | 638 AA. |
| Sequence status | Complete. |
| Protein existence | Evidence at protein level |
General annotation (Comments)
| Function | Involved in regulation of actin cytoskeleton organization and endocytosis. Ref.3 Ref.4 |
| Catalytic activity | ATP + a protein = ADP + a phosphoprotein. |
| Subunit structure | Interacts with ABP1, which is required for proper actin patch localization. Ref.5 |
| Subcellular location | Cytoplasm › cytoskeleton › actin patch. Note: Cortical actin patches. Ref.3 Ref.6 |
| Miscellaneous | Present with 1551 molecules/cell in log phase SD medium. Ref.7 |
| Sequence similarities | Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Contains 1 protein kinase domain. |
Ontologies
| Keywords | |
|---|---|
| Cellular component | Cytoplasm Cytoskeleton |
| Ligand | ATP-binding Nucleotide-binding |
| Molecular function | Kinase Serine/threonine-protein kinase Transferase |
| PTM | Phosphoprotein |
| Technical term | 3D-structure Complete proteome Reference proteome |
| Gene Ontology (GO) | |
| Biological process | actin cortical patch assembly Traceable author statement. Source: SGD actin filament organizationInferred from mutant phenotype Ref.3. Source: SGD cytokinesisTraceable author statement. Source: SGD regulation of endocytosisInferred from mutant phenotype. Source: SGD |
| Cellular component | actin cortical patch Inferred from direct assay Ref.3. Source: SGD mating projection tipInferred from direct assay. Source: SGD |
| Molecular function | ATP binding Inferred from electronic annotation. Source: UniProtKB-KW protein bindingInferred from physical interaction. Source: IntAct protein serine/threonine kinase activityInferred from direct assay. Source: SGD |
| Complete GO annotation... | |
Binary interactions
With | Entry | #Exp. | IntAct | Notes |
|---|---|---|---|---|
| ABP1 | P15891 | 4 | EBI-9817,EBI-2036 |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 638 | 638 | Actin-regulating kinase 1 | PRO_0000085635 | |||||
Regions | |||||||||
| Domain | 22 – 298 | 277 | Protein kinase | ||||||
| Nucleotide binding | 28 – 36 | 9 | ATP By similarity | ||||||
| Region | 602 – 615 | 14 | Interaction with SH3 domain of ABP1 | ||||||
Sites | |||||||||
| Active site | 159 | 1 | Proton acceptor By similarity | ||||||
| Binding site | 56 | 1 | ATP By similarity | ||||||
Amino acid modifications | |||||||||
| Modified residue | 478 | 1 | Phosphoserine Ref.8 Ref.10 | ||||||
| Modified residue | 502 | 1 | Phosphoserine Ref.10 | ||||||
| Modified residue | 522 | 1 | Phosphoserine Ref.10 | ||||||
| Modified residue | 535 | 1 | Phosphoserine Ref.9 Ref.10 | ||||||
| Modified residue | 568 | 1 | Phosphoserine Ref.10 | ||||||
| Modified residue | 569 | 1 | Phosphoserine Ref.10 | ||||||
Experimental info | |||||||||
| Mutagenesis | 56 | 1 | K → A: Abolishes protein kinase activity. Ref.3 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications." Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J. Hani J.Nature 387:93-98(1997) [PubMed: 9169873] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c. |
| [2] | Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c. |
| [3] | "Novel protein kinases Ark1p and Prk1p associate with and regulate the cortical actin cytoskeleton in budding yeast." Cope M.J.T.V., Yang S., Shang C., Drubin D.G. J. Cell Biol. 144:1203-1218(1999) [PubMed: 10087264] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, MUTAGENESIS OF LYS-56. |
| [4] | "In vivo role for actin-regulating kinases in endocytosis and yeast epsin phosphorylation." Watson H.A., Cope M.J.T.V., Groen A.C., Drubin D.G., Wendland B. Mol. Biol. Cell 12:3668-3679(2001) [PubMed: 11694597] [Abstract] Cited for: FUNCTION. |
| [5] | "Unusual binding properties of the SH3 domain of the yeast actin-binding protein Abp1: structural and functional analysis." Fazi B., Cope M.J.T.V., Douangamath A., Ferracuti S., Schirwitz K., Zucconi A., Drubin D.G., Wilmanns M., Cesareni G., Castagnoli L. J. Biol. Chem. 277:5290-5298(2002) [PubMed: 11668184] [Abstract] Cited for: INTERACTION WITH ABP1. |
| [6] | "Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS]. |
| [7] | "Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS]. |
| [8] | "Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry." Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F. Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed: 17287358] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-478, MASS SPECTROMETRY. |
| [9] | "Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed: 17563356] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-535, MASS SPECTROMETRY. |
| [10] | "A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-478; SER-502; SER-522; SER-535; SER-568 AND SER-569, MASS SPECTROMETRY. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| EMBL GenBank DDBJ | Z71296 Genomic DNA. Translation: CAA95882.1. BK006947 Genomic DNA. Translation: DAA10525.1. | ||||||||||||
| PIR | S62932. | ||||||||||||
| RefSeq | NP_014378.1. NM_001182859.1. | ||||||||||||
3D structure databases | |||||||||||||
| PDBe RCSB PDB PDBj |
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| ProteinModelPortal | P53974. | ||||||||||||
| SMR | P53974. Positions 11-301. | ||||||||||||
| ModBase | Search... | ||||||||||||
Protein-protein interaction databases | |||||||||||||
| DIP | DIP-4381N. | ||||||||||||
| IntAct | P53974. 17 interactions. | ||||||||||||
| MINT | MINT-511765. | ||||||||||||
| STRING | P53974. | ||||||||||||
Protocols and materials databases | |||||||||||||
| StructuralBiologyKnowledgebase | Search... | ||||||||||||
Genome annotation databases | |||||||||||||
| EnsemblFungi | YNL020C; YNL020C; YNL020C. | ||||||||||||
| GeneID | 855711. | ||||||||||||
| KEGG | sce:YNL020C. | ||||||||||||
| NMPDR | fig|4932.3.peg.5456. | ||||||||||||
Organism-specific databases | |||||||||||||
| CYGD | YNL020c. | ||||||||||||
| SGD | S000004965. ARK1. | ||||||||||||
Phylogenomic databases | |||||||||||||
| eggNOG | fuNOG04060. | ||||||||||||
| GeneTree | EFGT00050000000617. | ||||||||||||
| HOGENOM | HBG202709. | ||||||||||||
| OrthoDB | EOG4NCQNG. | ||||||||||||
Gene expression databases | |||||||||||||
| ArrayExpress | P53974. | ||||||||||||
| Genevestigator | P53974. | ||||||||||||
| GermOnline | YNL020C. Saccharomyces cerevisiae. | ||||||||||||
Family and domain databases | |||||||||||||
| InterPro | IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR017442. Se/Thr_kinase-like_dom. IPR008271. Ser/Thr_kinase_AS. [Graphical view] | ||||||||||||
| KO | K08286. | ||||||||||||
| Pfam | PF00069. Pkinase. 1 hit. [Graphical view] | ||||||||||||
| SUPFAM | SSF56112. Kinase_like. 1 hit. | ||||||||||||
| PROSITE | PS00107. PROTEIN_KINASE_ATP. False negative. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view] | ||||||||||||
| ProtoNet | Search... | ||||||||||||
Other | |||||||||||||
| NextBio | 980061. | ||||||||||||
Entry information
| Entry name | ARK1_YEAST | ||||||||
| Accession | Primary (citable) accession number: P53974 Secondary accession number(s): D6W1F9 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Fungal Protein Annotation Program | ||||||||
Relevant documents
| Yeast Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD |
| Yeast chromosome XIV Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names |
| PDB cross-references Index of Protein Data Bank (PDB) cross-references |
| SIMILARITY comments Index of protein domains and families |

Clusters with