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Protein

Actin-regulating kinase 1

Gene

ARK1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in regulation of actin cytoskeleton organization and endocytosis.2 Publications

Miscellaneous

Present with 1551 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei56ATPPROSITE-ProRule annotation1
Active sitei159Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi28 – 36ATPPROSITE-ProRule annotation9

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • protein serine/threonine kinase activity Source: SGD

GO - Biological processi

  • actin cortical patch assembly Source: SGD
  • actin filament organization Source: SGD
  • protein phosphorylation Source: SGD
  • regulation of endocytosis Source: SGD

Keywordsi

Molecular functionKinase, Serine/threonine-protein kinase, Transferase
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-33058-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Actin-regulating kinase 1 (EC:2.7.11.1)
Gene namesi
Name:ARK1
Ordered Locus Names:YNL020C
ORF Names:N2823
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIV

Organism-specific databases

EuPathDBiFungiDB:YNL020C
SGDiS000004965 ARK1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi56K → A: Abolishes protein kinase activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000856351 – 638Actin-regulating kinase 1Add BLAST638

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei478PhosphoserineCombined sources1
Modified residuei522PhosphoserineCombined sources1
Modified residuei535PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP53974
PaxDbiP53974
PRIDEiP53974

PTM databases

iPTMnetiP53974

Interactioni

Subunit structurei

Interacts with ABP1, which is required for proper actin patch localization.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
ABP1P1589112EBI-9817,EBI-2036

Protein-protein interaction databases

BioGridi35806, 157 interactors
DIPiDIP-4381N
IntActiP53974, 18 interactors
MINTiP53974
STRINGi4932.YNL020C

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2RPNNMR-B605-621[»]
ProteinModelPortaliP53974
SMRiP53974
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP53974

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini22 – 298Protein kinasePROSITE-ProRule annotationAdd BLAST277

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni602 – 615Interaction with SH3 domain of ABP1Add BLAST14

Sequence similaritiesi

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.PROSITE-ProRule annotation

Phylogenomic databases

GeneTreeiENSGT00880000137958
HOGENOMiHOG000247884
InParanoidiP53974
KOiK08853
OrthoDBiEOG092C0MJY

Family and domain databases

InterProiView protein in InterPro
IPR030078 Ark1/Prk1
IPR011009 Kinase-like_dom_sf
IPR000719 Prot_kinase_dom
IPR008271 Ser/Thr_kinase_AS
PANTHERiPTHR22967:SF82 PTHR22967:SF82, 3 hits
PfamiView protein in Pfam
PF00069 Pkinase, 1 hit
SMARTiView protein in SMART
SM00220 S_TKc, 1 hit
SUPFAMiSSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00108 PROTEIN_KINASE_ST, 1 hit

Sequencei

Sequence statusi: Complete.

P53974-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNQPQIGTYN VGTQLTVGSH QVEIIKYLTS GGFAQVYSAL INPPDPHSNS
60 70 80 90 100
SVACLKRVIV PDKPSLNTLR AEVDAMRLLK NNRYVVSYID SHAAKAMLHN
110 120 130 140 150
GSYEVFVLME YCERGGLIDF MNTRLQNRLH EFEILQIMSQ VTQGVAAMHA
160 170 180 190 200
LQPPLIHRDI KIENVLISAN NEYKLCDFGS VCGIIRPPRN SQELSYVQQD
210 220 230 240 250
ILKNTTAQYR SPEMIDTFRG LPIDEKSDIW ALGIFLYKLC YYTTPFEKGG
260 270 280 290 300
DLAILSGKFE FPLYPNYSEQ LKGLIRDILV QDPRHRPNVY QLLKRISIMQ
310 320 330 340 350
NVPCPINDIQ VVQAPSSHLN LTELHQLSAT QNILSLNSPT TMENTMPNAT
360 370 380 390 400
FQISMADNTT TAQMHPNRKP SQIAYDASFS NSAKGSQPLF DKSQNMYHAL
410 420 430 440 450
DPPLVEPLAS SVSNNDNELK ANSATKLKQA IVSEAHTFRQ NNSIDFPLQN
460 470 480 490 500
IIPQYEDSSS SSDESYSGDV DELKKTRSLG SYSTRGNIKK NQSVKESLTS
510 520 530 540 550
SSLPGTSFTP TSTKVNLKHE NSPFKSTFVN TIDNSKDDLN KPSYEDLDVS
560 570 580 590 600
KQNLKNSIQQ RMIDKLNSSE ESFNARKMSK VKLHEKGEID KPTMLKSSGP
610 620 630
ISKDKKTKPT PPPKPSHLKP KPPPKPLLLA GRKLSLDK
Length:638
Mass (Da):71,373
Last modified:October 1, 1996 - v1
Checksum:i139CA4CA1B6A181F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z71296 Genomic DNA Translation: CAA95882.1
BK006947 Genomic DNA Translation: DAA10525.1
PIRiS62932
RefSeqiNP_014378.1, NM_001182859.1

Genome annotation databases

EnsemblFungiiYNL020C; YNL020C; YNL020C
GeneIDi855711
KEGGisce:YNL020C

Similar proteinsi

Entry informationi

Entry nameiARK1_YEAST
AccessioniPrimary (citable) accession number: P53974
Secondary accession number(s): D6W1F9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: May 23, 2018
This is version 151 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

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