Reviewed, UniProtKB/Swiss-Prot P53973 (HDA1_YEAST)

Names and origin

Protein names

Recommended name:
    Histone deacetylase HDA1
    EC=3.5.1.98

Gene names

Name:	HDA1
Ordered Locus Names:	YNL021W
ORF Names:	N2819

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	706 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes.
Catalytic activity	Hydrolysis of an N(6)-acetyl-lysine residue of a histone to yield a deacetylated histone.
Subcellular location	Nucleus.
Miscellaneous	Present with 3050 molecules/cell in log phase SD medium. Ref.3
Sequence similarities	Belongs to the histone deacetylase family. Type 2 subfamily.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Molecular function	Chromatin regulator Hydrolase Repressor
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	chromatin organization during transcription Inferred from mutant phenotype. Source: SGD gene silencing by RNA Inferred from mutant phenotype. Source: SGD histone deacetylation Ref.2 Inferred from direct assay. Source: SGD loss of chromatin silencing during replicative cell aging Inferred from genetic interaction. Source: SGD negative regulation of gene-specific transcription from RNA polymerase II promoter Inferred from mutant phenotype. Source: SGD negative regulation of transcription by transcription factor localization Inferred from genetic interaction. Source: SGD positive regulation of gene-specific transcription from RNA polymerase II promoter Inferred from mutant phenotype. Source: SGD regulation of chromatin silencing at telomere Ref.2 Inferred from mutant phenotype. Source: SGD transcription Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	HDA1 complex Ref.2 Inferred from direct assay. Source: SGD
Molecular function	chromatin binding Inferred from direct assay. Source: SGD hydrolase activity Inferred from electronic annotation. Source: UniProtKB-KW protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
GZF3	P42944	2	EBI-8206,EBI-8042
TUP1	P16649	1	EBI-8206,EBI-19654

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 706	706	Histone deacetylase HDA1		PRO_0000114738
Regions
	Region	67 – 396	330	Histone deacetylase
Sites
	Active site	206	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	P53973-1 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 4E7069E66D03264D	FASTA	706	80,070
	10 20 30 40 50 60 MDSVMVKKEV LENPDHDLKR KLEENKEEEN SLSTTSKSKR QVIVPVCMPK IHYSPLKTGL 70 80 90 100 110 120 CYDVRMRYHA KIFTSYFEYI DPHPEDPRRI YRIYKILAEN GLINDPTLSG VDDLGDLMLK 130 140 150 160 170 180 IPVRAATSEE ILEVHTKEHL EFIESTEKMS REELLKETEK GDSVYFNNDS YASARLPCGG 190 200 210 220 230 240 AIEACKAVVE GRVKNSLAVV RPPGHHAEPQ AAGGFCLFSN VAVAAKNILK NYPESVRRIM 250 260 270 280 290 300 ILDWDIHHGN GTQKSFYQDD QVLYVSLHRF EMGKYYPGTI QGQYDQTGEG KGEGFNCNIT 310 320 330 340 350 360 WPVGGVGDAE YMWAFEQVVM PMGREFKPDL VIISSGFDAA DGDTIGQCHV TPSCYGHMTH 370 380 390 400 410 420 MLKSLARGNL CVVLEGGYNL DAIARSALSV AKVLIGEPPD ELPDPLSDPK PEVIEMIDKV 430 440 450 460 470 480 IRLQSKYWNC FRRRHANSGC NFNEPINDSI ISKNFPLQKA IRQQQQHYLS DEFNFVTLPL 490 500 510 520 530 540 VSMDLPDNTV LCTPNISESN TIIIVVHDTS DIWAKRNVIS GTIDLSSSVI IDNSLDFIKW 550 560 570 580 590 600 GLDRKYGIID VNIPLTLFEP DNYSGMITSQ EVLIYLWDNY IKYFPSVAKI AFIGIGDSYS 610 620 630 640 650 660 GIVHLLGHRD TRAVTKTVIN FLGDKQLKPL VPLVDETLSE WYFKNSLIFS NNSHQCWKEN 670 680 690 700 ESRKPRKKFG RVLRCDTDGL NNIIEERFEE ATDFILDSFE EWSDEE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications." Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J. , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J. Nature 387:93-98(1997) [PubMed: 9169873] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	"HDA1 and RPD3 are members of distinct yeast histone deacetylase complexes that regulate silencing and transcription." Rundlett S.E., Carmen A.A., Kobayashi R., Bavykin S., Turner B.M., Grunstein M. Proc. Natl. Acad. Sci. U.S.A. 93:14503-14508(1996) [PubMed: 8962081] [Abstract] Cited for: CHARACTERIZATION.
[3]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z71297 Genomic DNA. Translation: CAA95883.1.
PIR	S62933.
RefSeq	NP_014377.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:1360N.
IntAct	P53973. 17 interactions.
STRING	P53973.
Proteomic databases
PeptideAtlas	P53973.
Genome annotation databases
Ensembl	YNL021W; YNL021W; YNL021W; Saccharomyces cerevisiae. [Genome view]
GeneID	855710.
GenomeReviews	Gene locus YNL021W in contig Y13139_GR.
KEGG	sce:YNL021W.
NMPDR	fig|4932.3.peg.5455.
Organism-specific databases
CYGD	YNL021w.
SGD	S000004966. HDA1.
Phylogenomic databases
HOGENOM	P53973.
OMA	DPHPEDP.
Gene expression databases
ArrayExpress	P53973.
Genevestigator	P53973.
GermOnline	YNL021W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR019154. Arb2_domain. IPR000286. His_deacetylse. IPR017321. Hist_deAcase_II_yeast. [Graphical view]
Gene3D	G3DSA:3.40.800.20. His_deacetylse. 1 hit.
PANTHER	PTHR10625. His_deacetylse. 1 hit.
Pfam	PF09757. Arb2. 1 hit. PF00850. Hist_deacetyl. 1 hit. [Graphical view]
PIRSF	PIRSF037919. HDAC_II_yeast. 1 hit.
PRINTS	PR01270. HDASUPER.
ProtoNet	Search...
Other Resources
NextBio	980058.

Entry information

Entry name

HDA1_YEAST

Accession

Primary (citable) accession number: P53973

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	November 3, 2009
This is version 71 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)