P53971 (FAP1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
FKBP12-associated protein 1

Gene names

Name:	FAP1
Ordered Locus Names:	YNL023C
ORF Names:	N2812

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	965 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May play a role in transcription regulation By similarity. Ref.4
Subunit structure	Interacts with FPR1. Ref.4
Subcellular location	Cytoplasm. Nucleus. Note: Translocates to the nucleus in response to rapamycin. Ref.4
Miscellaneous	Present with 589 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the NFX1 family. Contains 5 NF-X1-type zinc fingers. Contains 1 R3H domain. Contains 1 RING-type zinc finger.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Cytoplasm Nucleus
Domain	Repeat Zinc-finger
Ligand	Metal-binding Zinc
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from direct assay Ref.4. Source: SGD nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	nucleic acid binding Inferred from electronic annotation. Source: InterPro sequence-specific DNA binding transcription factor activity Inferred from sequence or structural similarity Ref.4. Source: SGD zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
FPR1	P20081	3	EBI-28638,EBI-6961

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 965	965	FKBP12-associated protein 1		PRO_0000056343
Regions
	Domain	733 – 796	64	R3H
	Zinc finger	68 – 118	51	RING-type; degenerate
	Zinc finger	159 – 177	19	NF-X1-type 1
	Zinc finger	216 – 235	20	NF-X1-type 2
	Zinc finger	362 – 382	21	NF-X1-type 3
	Zinc finger	468 – 487	20	NF-X1-type 4
	Zinc finger	586 – 606	21	NF-X1-type 5
Amino acid modifications
	Modified residue	958	1	Phosphoserine Ref.5

Sequences

	Sequence		Length	Mass (Da)
	P53971 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 121C57BB07C6FA9D	FASTA	965	108,495
	10 20 30 40 50 60 MTEHESLGLE QNQDGGDTYR HHNLSDGCIS SVEDANEQPS SYEEESDDDM QYYERAIQEI 70 80 90 100 110 120 SSGDSYVCMI CTVEMDYTCQ MFACKRCYRV FDYGCIREWA LKSTEKTVDR IWKCPNCYYV 130 140 150 160 170 180 SKRVPVKNRP TCWCGKVVNP DPNPLDPNSC GQTCNASTCM HGCSKICHLG PHPECTRMVE 190 200 210 220 230 240 IMCHCGKHSK SIFCYQSKVM KKNFNCQEVC GLPLSCSIHT CKKKCHPGLC GPCPEMIISK 250 260 270 280 290 300 DSPKKQIKCY CGNHTRANIK CSETKFPKSG KSSKDENGNR WIGVFACADN RVVDYSCRKH 310 320 330 340 350 360 SFIESCISPP TINGEKACPF LPSSLKTCPC GRTALEELTK PRKHCDDPIP TCDSRCGKPL 370 380 390 400 410 420 KCGKHSCPFT CHDKACMEPC LQIDSVKCAC EQSTFSVPCG FQGRPRCNIK CESLMSCRRH 430 440 450 460 470 480 RCTDRCCSGR PSAIRRKKNL FRTQDLLDES LVEAKHICLK PCNLTLSCGI HKCQRKCHPG 490 500 510 520 530 540 KCPPCLESDS NDLVCPCGNT VVPAPVRCGT KLPTCNHPCI KVVRGESTCG HKPMPHTCHS 550 560 570 580 590 600 LDVSCPPCTE TVFKPCKCGK KTKVRTVCFQ TDVSCGIKCG IPLSYCYHTC QKTCHLPGNC 610 620 630 640 650 660 QKVCKQTCGQ KRLNCNHECP KPCHGKTECP DLPCATLVKI YCKCGRIKKS VTCGAKSDRV 670 680 690 700 710 720 SVTESSVLDC NEECEALKRL KELKEAFGIK EETNNFTSNE LDALKKLVSV ATTFEELQLP 730 740 750 760 770 780 FTEAALSVYS KQERWCSQIE AILNKLMDDK TRSSLHFKPM RPPQRHFIRE LAKAYGLYSE 790 800 810 820 830 840 SQDREPMRSV FIKKEDNGAS NKPVLSLAEA YPLYESFKQL QKERKAQEFQ ARTTAKLINF 850 860 870 880 890 900 EVQDTEPKVE VAKKNGFLVQ NLVAGNTAED LRRFFEPHLK HTLVVNPQYL ILDDGKTALV 910 920 930 940 950 960 YPENYETASV NTERDMELLV GHFDFMAKEA FLADSISLCS TEEELERRLD TPVIQEDSPV MDNNT

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications." Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J. , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J. Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[4]	"FAP1, a homologue of human transcription factor NF-X1, competes with rapamycin for binding to FKBP12 in yeast." Kunz J., Loeschmann A., Deuter-Reinhard M., Hall M.N. Mol. Microbiol. 37:1480-1493(2000) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH FPR1, SUBCELLULAR LOCATION.
[5]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-958, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z71299 Genomic DNA. Translation: CAA95885.1. BK006947 Genomic DNA. Translation: DAA10522.1.
PIR	S62935.
RefSeq	NP_014375.1. NM_001182862.1.
3D structure databases
ProteinModelPortal	P53971.
SMR	P53971. Positions 737-795.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-967N.
IntAct	P53971. 14 interactions.
MINT	MINT-388122.
STRING	4932.YNL023C.
Proteomic databases
PaxDb	P53971.
PeptideAtlas	P53971.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YNL023C; YNL023C; YNL023C.
GeneID	855708.
KEGG	sce:YNL023C.
Organism-specific databases
CYGD	YNL023c.
SGD	S000004968. FAP1.
Phylogenomic databases
eggNOG	NOG12793.
GeneTree	ENSGT00400000022083.
HOGENOM	HOG000112499.
OMA	LVRYCPC.
OrthoDB	EOG4TQQJ1.
Gene expression databases
Genevestigator	P53971.
GermOnline	YNL023C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR001374. R3H_ss-bd. IPR011011. Znf_FYVE_PHD. IPR000967. Znf_NFX1. IPR001841. Znf_RING. [Graphical view]
Pfam	PF01424. R3H. 1 hit. PF01422. zf-NF-X1. 2 hits. [Graphical view]
SMART	SM00393. R3H. 1 hit. SM00184. RING. 1 hit. SM00438. ZnF_NFX. 7 hits. [Graphical view]
SUPFAM	SSF57903. FYVE_PHD_ZnF. 1 hit.
PROSITE	PS51061. R3H. 1 hit. PS00518. ZF_RING_1. False negative. PS50089. ZF_RING_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	980052.

Entry information

Entry name

FAP1_YEAST

Accession

Primary (citable) accession number: P53971
Secondary accession number(s): D6W1F6

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	May 1, 2013
This is version 110 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program