ID   SAM50_YEAST             Reviewed;         484 AA.
AC   P53969; D6W1F2;
DT   01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1996, sequence version 1.
DT   27-MAR-2024, entry version 172.
DE   RecName: Full=Sorting assembly machinery 50 kDa subunit;
DE   AltName: Full=TOB complex 55 kDa subunit;
GN   Name=SAM50; Synonyms=TOB55; OrderedLocusNames=YNL026W; ORFNames=N2802;
OS   Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX   NCBI_TaxID=559292;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=9169873;
RA   Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K.,
RA   Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K.,
RA   Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M.,
RA   Beinhauer J.D., Boskovic J., Buitrago M.J., Bussereau F., Coster F.,
RA   Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F.,
RA   Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C.,
RA   Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A.,
RA   Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H.,
RA   Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L.,
RA   Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R.,
RA   Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D.,
RA   Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A.,
RA   Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C.,
RA   Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F.,
RA   Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G.,
RA   Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M.,
RA   Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.;
RT   "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its
RT   evolutionary implications.";
RL   Nature 387:93-98(1997).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=24374639; DOI=10.1534/g3.113.008995;
RA   Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA   Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA   Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT   "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL   G3 (Bethesda) 4:389-398(2014).
RN   [3]
RP   IDENTIFICATION BY MASS SPECTROMETRY, FUNCTION, SUBCELLULAR LOCATION, AND
RP   IDENTIFICATION IN THE SAM COMPLEX.
RX   PubMed=14570913; DOI=10.1074/jbc.c300442200;
RA   Kozjak V., Wiedemann N., Milenkovic D., Lohaus C., Meyer H.E., Guiard B.,
RA   Meisinger C., Pfanner N.;
RT   "An essential role of Sam50 in the protein sorting and assembly machinery
RT   of the mitochondrial outer membrane.";
RL   J. Biol. Chem. 278:48520-48523(2003).
RN   [4]
RP   IDENTIFICATION IN THE SAM COMPLEX.
RX   PubMed=14685243; DOI=10.1038/nature02208;
RA   Paschen S.A., Waizenegger T., Stan T., Preuss M., Cyrklaff M., Hell K.,
RA   Rapaport D., Neupert W.;
RT   "Evolutionary conservation of biogenesis of beta-barrel membrane
RT   proteins.";
RL   Nature 426:862-866(2003).
RN   [5]
RP   IDENTIFICATION IN THE SAM COMPLEX.
RX   PubMed=15590639; DOI=10.1074/jbc.m411510200;
RA   Habib S.J., Waizenegger T., Lech M., Neupert W., Rapaport D.;
RT   "Assembly of the TOB complex of mitochondria.";
RL   J. Biol. Chem. 280:6434-6440(2005).
CC   -!- FUNCTION: Component of the mitochondrial outer membrane sorting
CC       assembly machinery (SAM or TOB) complex, which is required for the
CC       sorting of proteins with complicated topology, such as beta-barrel
CC       proteins, to the mitochondrial outer membrane after import by the TOM
CC       complex. {ECO:0000269|PubMed:14570913}.
CC   -!- SUBUNIT: Component of the mitochondrial outer membrane sorting assembly
CC       machinery (SAM or TOB) complex, which at least consists of SAM35, SAM37
CC       and SAM50 (PubMed:14570913, PubMed:14685243, PubMed:15590639).
CC       Associates with the mitochondrial contact site and cristae organizing
CC       system (MICOS) complex (also known as MINOS or MitOS complex) (By
CC       similarity). {ECO:0000250, ECO:0000269|PubMed:14570913,
CC       ECO:0000269|PubMed:14685243, ECO:0000269|PubMed:15590639}.
CC   -!- INTERACTION:
CC       P53969; P18409: MDM10; NbExp=4; IntAct=EBI-28646, EBI-10580;
CC       P53969; P14693: SAM35; NbExp=8; IntAct=EBI-28646, EBI-24602;
CC       P53969; P50110: SAM37; NbExp=5; IntAct=EBI-28646, EBI-2347180;
CC       P53969; P49334: TOM22; NbExp=6; IntAct=EBI-28646, EBI-12527;
CC       P53969; P23644: TOM40; NbExp=4; IntAct=EBI-28646, EBI-12539;
CC   -!- SUBCELLULAR LOCATION: Mitochondrion outer membrane
CC       {ECO:0000269|PubMed:14570913}; Multi-pass membrane protein
CC       {ECO:0000305|PubMed:14685243}.
CC   -!- DOMAIN: Its C-terminal part seems to contain many membrane-spanning
CC       sided beta-sheets, that have the potential to adopt a transmembrane
CC       beta-barrel type structure. {ECO:0000305|PubMed:14685243}.
CC   -!- SIMILARITY: Belongs to the SAM50/omp85 family. {ECO:0000305}.
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DR   EMBL; Z71302; CAA95888.1; -; Genomic_DNA.
DR   EMBL; BK006947; DAA10518.1; -; Genomic_DNA.
DR   PIR; S62938; S62938.
DR   RefSeq; NP_014372.1; NM_001182865.1.
DR   PDB; 7E4H; EM; 3.01 A; A=2-484.
DR   PDB; 7E4I; EM; 3.05 A; A=2-484.
DR   PDB; 7VKU; EM; 3.20 A; A=1-484.
DR   PDBsum; 7E4H; -.
DR   PDBsum; 7E4I; -.
DR   PDBsum; 7VKU; -.
DR   AlphaFoldDB; P53969; -.
DR   EMDB; EMD-30985; -.
DR   EMDB; EMD-30986; -.
DR   EMDB; EMD-32019; -.
DR   SMR; P53969; -.
DR   BioGRID; 35800; 390.
DR   ComplexPortal; CPX-1744; Mitochondrial sorting and assembly machinery complex.
DR   IntAct; P53969; 14.
DR   MINT; P53969; -.
DR   STRING; 4932.YNL026W; -.
DR   TCDB; 1.B.33.3.1; the outer membrane protein insertion porin (bam complex) (ompip) family.
DR   MaxQB; P53969; -.
DR   PaxDb; 4932-YNL026W; -.
DR   PeptideAtlas; P53969; -.
DR   EnsemblFungi; YNL026W_mRNA; YNL026W; YNL026W.
DR   GeneID; 855705; -.
DR   KEGG; sce:YNL026W; -.
DR   AGR; SGD:S000004971; -.
DR   SGD; S000004971; SAM50.
DR   VEuPathDB; FungiDB:YNL026W; -.
DR   eggNOG; KOG2602; Eukaryota.
DR   GeneTree; ENSGT00390000011355; -.
DR   HOGENOM; CLU_014798_3_1_1; -.
DR   InParanoid; P53969; -.
DR   OMA; MNDDFIT; -.
DR   OrthoDB; 46657at2759; -.
DR   BioCyc; YEAST:G3O-33064-MONOMER; -.
DR   BioGRID-ORCS; 855705; 2 hits in 10 CRISPR screens.
DR   PRO; PR:P53969; -.
DR   Proteomes; UP000002311; Chromosome XIV.
DR   RNAct; P53969; Protein.
DR   GO; GO:0005741; C:mitochondrial outer membrane; IDA:SGD.
DR   GO; GO:0005739; C:mitochondrion; IDA:ComplexPortal.
DR   GO; GO:0001401; C:SAM complex; IDA:SGD.
DR   GO; GO:0008320; F:protein transmembrane transporter activity; IDA:SGD.
DR   GO; GO:0030150; P:protein import into mitochondrial matrix; IDA:ComplexPortal.
DR   GO; GO:0045040; P:protein insertion into mitochondrial outer membrane; IMP:SGD.
DR   GO; GO:0065003; P:protein-containing complex assembly; IMP:SGD.
DR   Gene3D; 2.40.160.50; membrane protein fhac: a member of the omp85/tpsb transporter family; 1.
DR   InterPro; IPR000184; Bac_surfAg_D15.
DR   InterPro; IPR039910; D15-like.
DR   PANTHER; PTHR12815:SF18; SORTING AND ASSEMBLY MACHINERY COMPONENT 50 HOMOLOG; 1.
DR   PANTHER; PTHR12815; SORTING AND ASSEMBLY MACHINERY SAMM50 PROTEIN FAMILY MEMBER; 1.
DR   Pfam; PF01103; Omp85; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Membrane; Mitochondrion; Mitochondrion outer membrane;
KW   Protein transport; Reference proteome; Transmembrane;
KW   Transmembrane beta strand; Transport.
FT   CHAIN           1..484
FT                   /note="Sorting assembly machinery 50 kDa subunit"
FT                   /id="PRO_0000215943"
FT   STRAND          30..38
FT                   /evidence="ECO:0007829|PDB:7E4H"
FT   HELIX           44..54
FT                   /evidence="ECO:0007829|PDB:7E4H"
FT   TURN            55..57
FT                   /evidence="ECO:0007829|PDB:7E4H"
FT   HELIX           61..77
FT                   /evidence="ECO:0007829|PDB:7E4H"
FT   STRAND          84..87
FT                   /evidence="ECO:0007829|PDB:7E4H"
FT   STRAND          109..116
FT                   /evidence="ECO:0007829|PDB:7E4H"
FT   STRAND          124..131
FT                   /evidence="ECO:0007829|PDB:7E4H"
FT   STRAND          133..135
FT                   /evidence="ECO:0007829|PDB:7E4H"
FT   STRAND          137..146
FT                   /evidence="ECO:0007829|PDB:7E4H"
FT   TURN            149..151
FT                   /evidence="ECO:0007829|PDB:7E4H"
FT   STRAND          156..181
FT                   /evidence="ECO:0007829|PDB:7E4H"
FT   STRAND          184..196
FT                   /evidence="ECO:0007829|PDB:7E4H"
FT   HELIX           199..201
FT                   /evidence="ECO:0007829|PDB:7E4H"
FT   STRAND          203..216
FT                   /evidence="ECO:0007829|PDB:7E4H"
FT   STRAND          235..251
FT                   /evidence="ECO:0007829|PDB:7E4H"
FT   TURN            255..257
FT                   /evidence="ECO:0007829|PDB:7E4H"
FT   HELIX           264..267
FT                   /evidence="ECO:0007829|PDB:7E4H"
FT   STRAND          271..285
FT                   /evidence="ECO:0007829|PDB:7E4H"
FT   STRAND          290..292
FT                   /evidence="ECO:0007829|PDB:7E4H"
FT   STRAND          295..306
FT                   /evidence="ECO:0007829|PDB:7E4H"
FT   TURN            307..309
FT                   /evidence="ECO:0007829|PDB:7E4H"
FT   STRAND          310..322
FT                   /evidence="ECO:0007829|PDB:7E4H"
FT   STRAND          329..342
FT                   /evidence="ECO:0007829|PDB:7E4H"
FT   HELIX           345..347
FT                   /evidence="ECO:0007829|PDB:7E4H"
FT   HELIX           353..355
FT                   /evidence="ECO:0007829|PDB:7E4H"
FT   TURN            362..364
FT                   /evidence="ECO:0007829|PDB:7E4H"
FT   STRAND          376..381
FT                   /evidence="ECO:0007829|PDB:7E4H"
FT   STRAND          384..397
FT                   /evidence="ECO:0007829|PDB:7E4H"
FT   STRAND          403..420
FT                   /evidence="ECO:0007829|PDB:7E4H"
FT   HELIX           427..433
FT                   /evidence="ECO:0007829|PDB:7E4H"
FT   STRAND          435..437
FT                   /evidence="ECO:0007829|PDB:7E4H"
FT   STRAND          441..449
FT                   /evidence="ECO:0007829|PDB:7E4H"
FT   STRAND          454..466
FT                   /evidence="ECO:0007829|PDB:7E4H"
FT   STRAND          474..481
FT                   /evidence="ECO:0007829|PDB:7E4H"
SQ   SEQUENCE   484 AA;  54406 MW;  651A1FE08A1F1845 CRC64;
     MTSSSGVDNE ISLDSPMPIF NESSTLKPIR VAGVVTTGTD HIDPSVLQAY LDDTIMKSIT
     LGQLVKNADV LNKRLCQHHI ALNAKQSFHF QGNTYISDEK ETHDVVPLME VVSQLDILPP
     KTFTAKTGTN FGNDNDAEAY LQFEKLIDKK YLKLPTRVNL EILRGTKIHS SFLFNSYSSL
     SPQSILNLKV FSQFYNWNTN KGLDIGQRGA RLSLRYEPLF LHKLLHNPHS NESPTLFHEW
     FLETCWRSTK ICSQGTSAPY MYSGTMLSQA GDQLRTILGH TFVLDKRDHI MCPTKGSMLK
     WSNELSPGKH LKTQLELNSV KSWMNDDFIT FSTTIKTGYL KNLSSQQSLP VHICDKFQSG
     GPSDIRGFQT FGLGPRDLYD AVGGDAFVSY GLSVFSRLPW KKVEKSNFRL HWFFNGGKLV
     NHDNTSLGNC IGQLSKEHST STGIGLVLRH PMARFELNFT LPITAHENDL IRKGFQFGLG
     LAFL
//