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P53969

- SAM50_YEAST

UniProt

P53969 - SAM50_YEAST

Protein

Sorting assembly machinery 50 kDa subunit

Gene

SAM50

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 108 (01 Oct 2014)
      Sequence version 1 (01 Oct 1996)
      Previous versions | rss
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    Functioni

    Component of the mitochondrial outer membrane sorting assembly machinery (SAM or TOB) complex, which is required for the sorting of proteins with complicated topology, such as beta-barrel proteins, to the mitochondrial outer membrane after import by the TOM complex.1 Publication

    GO - Molecular functioni

    1. protein binding Source: IntAct
    2. protein channel activity Source: SGD

    GO - Biological processi

    1. protein complex assembly Source: SGD
    2. protein import into mitochondrial outer membrane Source: SGD

    Keywords - Biological processi

    Protein transport, Transport

    Enzyme and pathway databases

    BioCyciYEAST:G3O-33064-MONOMER.
    ReactomeiREACT_189012. Mitochondrial protein import.

    Protein family/group databases

    TCDBi1.B.33.3.1. the outer membrane protein insertion porin (bam complex) (ompip) family.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Sorting assembly machinery 50 kDa subunit
    Alternative name(s):
    TOB complex 55 kDa subunit
    Gene namesi
    Name:SAM50
    Synonyms:TOB55
    Ordered Locus Names:YNL026W
    ORF Names:N2802
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XIV

    Organism-specific databases

    CYGDiYNL026w.
    SGDiS000004971. SAM50.

    Subcellular locationi

    GO - Cellular componenti

    1. integral component of mitochondrial outer membrane Source: SGD
    2. mitochondrial outer membrane Source: Reactome
    3. mitochondrial sorting and assembly machinery complex Source: SGD

    Keywords - Cellular componenti

    Membrane, Mitochondrion, Mitochondrion outer membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 484484Sorting assembly machinery 50 kDa subunitPRO_0000215943Add
    BLAST

    Proteomic databases

    MaxQBiP53969.
    PaxDbiP53969.
    PeptideAtlasiP53969.

    Expressioni

    Gene expression databases

    GenevestigatoriP53969.

    Interactioni

    Subunit structurei

    Component of the mitochondrial outer membrane sorting assembly machinery (SAM or TOB) complex, which at least consists of SAM35, SAM37 and SAM50. Associates with the mitochondrial inner membrane MINOS/MitOS complex By similarity.By similarity

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    MDM10P184094EBI-28646,EBI-10580
    SAM35P146938EBI-28646,EBI-24602
    SAM37P501105EBI-28646,EBI-2347180
    TOM22P493346EBI-28646,EBI-12527
    TOM40P236444EBI-28646,EBI-12539

    Protein-protein interaction databases

    BioGridi35800. 13 interactions.
    IntActiP53969. 13 interactions.
    MINTiMINT-4791160.
    STRINGi4932.YNL026W.

    Structurei

    3D structure databases

    ProteinModelPortaliP53969.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domaini

    Its C-terminal part seems to contain many membrane-spanning sided beta-sheets, that have the potential to adopt a transmembrane beta-barrel type structure.

    Sequence similaritiesi

    Belongs to the SAM50/omp85 family.Curated

    Keywords - Domaini

    Transmembrane, Transmembrane beta strand

    Phylogenomic databases

    eggNOGiCOG4775.
    GeneTreeiENSGT00390000011355.
    HOGENOMiHOG000170308.
    KOiK07277.
    OMAiHENDLIR.
    OrthoDBiEOG7W9S4R.

    Family and domain databases

    InterProiIPR000184. Bac_surfAg_D15.
    [Graphical view]
    PfamiPF01103. Bac_surface_Ag. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P53969-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTSSSGVDNE ISLDSPMPIF NESSTLKPIR VAGVVTTGTD HIDPSVLQAY    50
    LDDTIMKSIT LGQLVKNADV LNKRLCQHHI ALNAKQSFHF QGNTYISDEK 100
    ETHDVVPLME VVSQLDILPP KTFTAKTGTN FGNDNDAEAY LQFEKLIDKK 150
    YLKLPTRVNL EILRGTKIHS SFLFNSYSSL SPQSILNLKV FSQFYNWNTN 200
    KGLDIGQRGA RLSLRYEPLF LHKLLHNPHS NESPTLFHEW FLETCWRSTK 250
    ICSQGTSAPY MYSGTMLSQA GDQLRTILGH TFVLDKRDHI MCPTKGSMLK 300
    WSNELSPGKH LKTQLELNSV KSWMNDDFIT FSTTIKTGYL KNLSSQQSLP 350
    VHICDKFQSG GPSDIRGFQT FGLGPRDLYD AVGGDAFVSY GLSVFSRLPW 400
    KKVEKSNFRL HWFFNGGKLV NHDNTSLGNC IGQLSKEHST STGIGLVLRH 450
    PMARFELNFT LPITAHENDL IRKGFQFGLG LAFL 484
    Length:484
    Mass (Da):54,406
    Last modified:October 1, 1996 - v1
    Checksum:i651A1FE08A1F1845
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z71302 Genomic DNA. Translation: CAA95888.1.
    BK006947 Genomic DNA. Translation: DAA10518.1.
    PIRiS62938.
    RefSeqiNP_014372.1. NM_001182865.1.

    Genome annotation databases

    EnsemblFungiiYNL026W; YNL026W; YNL026W.
    GeneIDi855705.
    KEGGisce:YNL026W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z71302 Genomic DNA. Translation: CAA95888.1 .
    BK006947 Genomic DNA. Translation: DAA10518.1 .
    PIRi S62938.
    RefSeqi NP_014372.1. NM_001182865.1.

    3D structure databases

    ProteinModelPortali P53969.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 35800. 13 interactions.
    IntActi P53969. 13 interactions.
    MINTi MINT-4791160.
    STRINGi 4932.YNL026W.

    Protein family/group databases

    TCDBi 1.B.33.3.1. the outer membrane protein insertion porin (bam complex) (ompip) family.

    Proteomic databases

    MaxQBi P53969.
    PaxDbi P53969.
    PeptideAtlasi P53969.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YNL026W ; YNL026W ; YNL026W .
    GeneIDi 855705.
    KEGGi sce:YNL026W.

    Organism-specific databases

    CYGDi YNL026w.
    SGDi S000004971. SAM50.

    Phylogenomic databases

    eggNOGi COG4775.
    GeneTreei ENSGT00390000011355.
    HOGENOMi HOG000170308.
    KOi K07277.
    OMAi HENDLIR.
    OrthoDBi EOG7W9S4R.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-33064-MONOMER.
    Reactomei REACT_189012. Mitochondrial protein import.

    Miscellaneous databases

    NextBioi 980043.
    PROi P53969.

    Gene expression databases

    Genevestigatori P53969.

    Family and domain databases

    InterProi IPR000184. Bac_surfAg_D15.
    [Graphical view ]
    Pfami PF01103. Bac_surface_Ag. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications."
      Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J.
      , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.
      Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    3. "An essential role of Sam50 in the protein sorting and assembly machinery of the mitochondrial outer membrane."
      Kozjak V., Wiedemann N., Milenkovic D., Lohaus C., Meyer H.E., Guiard B., Meisinger C., Pfanner N.
      J. Biol. Chem. 278:48520-48523(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY, FUNCTION, SUBCELLULAR LOCATION, IDENTIFICATION IN THE SAM COMPLEX.
    4. "Evolutionary conservation of biogenesis of beta-barrel membrane proteins."
      Paschen S.A., Waizenegger T., Stan T., Preuss M., Cyrklaff M., Hell K., Rapaport D., Neupert W.
      Nature 426:862-866(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION IN THE SAM COMPLEX.
    5. Cited for: IDENTIFICATION IN THE SAM COMPLEX.

    Entry informationi

    Entry nameiSAM50_YEAST
    AccessioniPrimary (citable) accession number: P53969
    Secondary accession number(s): D6W1F2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 108 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Uncharacterized protein families (UPF)
      List of uncharacterized protein family (UPF) entries
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome XIV
      Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

    External Data

    Dasty 3