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Protein

Sorting assembly machinery 50 kDa subunit

Gene

SAM50

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the mitochondrial outer membrane sorting assembly machinery (SAM or TOB) complex, which is required for the sorting of proteins with complicated topology, such as beta-barrel proteins, to the mitochondrial outer membrane after import by the TOM complex.1 Publication

GO - Molecular functioni

  • protein transmembrane transporter activity Source: SGD

GO - Biological processi

  • cellular protein-containing complex assembly Source: SGD
  • protein-containing complex assembly Source: SGD
  • protein import into mitochondrial outer membrane Source: SGD

Keywordsi

Biological processProtein transport, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-33064-MONOMER

Protein family/group databases

TCDBi1.B.33.3.1 the outer membrane protein insertion porin (bam complex) (ompip) family

Names & Taxonomyi

Protein namesi
Recommended name:
Sorting assembly machinery 50 kDa subunit
Alternative name(s):
TOB complex 55 kDa subunit
Gene namesi
Name:SAM50
Synonyms:TOB55
Ordered Locus Names:YNL026W
ORF Names:N2802
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIV

Organism-specific databases

EuPathDBiFungiDB:YNL026W
SGDiS000004971 SAM50

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion outer membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002159431 – 484Sorting assembly machinery 50 kDa subunitAdd BLAST484

Proteomic databases

MaxQBiP53969
PaxDbiP53969
PRIDEiP53969

Interactioni

Subunit structurei

Component of the mitochondrial outer membrane sorting assembly machinery (SAM or TOB) complex, which at least consists of SAM35, SAM37 and SAM50. Associates with the mitochondrial contact site and cristae organizing system (MICOS) complex (also known as MINOS or MitOS complex) (By similarity).By similarity

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi35800, 16 interactors
IntActiP53969, 20 interactors
MINTiP53969
STRINGi4932.YNL026W

Structurei

3D structure databases

ProteinModelPortaliP53969
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domaini

Its C-terminal part seems to contain many membrane-spanning sided beta-sheets, that have the potential to adopt a transmembrane beta-barrel type structure.

Sequence similaritiesi

Belongs to the SAM50/omp85 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane beta strand

Phylogenomic databases

GeneTreeiENSGT00390000011355
HOGENOMiHOG000170308
InParanoidiP53969
KOiK07277
OMAiHWFLNGG
OrthoDBiEOG092C3G4O

Family and domain databases

InterProiView protein in InterPro
IPR000184 Bac_surfAg_D15
PfamiView protein in Pfam
PF01103 Bac_surface_Ag, 1 hit

Sequencei

Sequence statusi: Complete.

P53969-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTSSSGVDNE ISLDSPMPIF NESSTLKPIR VAGVVTTGTD HIDPSVLQAY
60 70 80 90 100
LDDTIMKSIT LGQLVKNADV LNKRLCQHHI ALNAKQSFHF QGNTYISDEK
110 120 130 140 150
ETHDVVPLME VVSQLDILPP KTFTAKTGTN FGNDNDAEAY LQFEKLIDKK
160 170 180 190 200
YLKLPTRVNL EILRGTKIHS SFLFNSYSSL SPQSILNLKV FSQFYNWNTN
210 220 230 240 250
KGLDIGQRGA RLSLRYEPLF LHKLLHNPHS NESPTLFHEW FLETCWRSTK
260 270 280 290 300
ICSQGTSAPY MYSGTMLSQA GDQLRTILGH TFVLDKRDHI MCPTKGSMLK
310 320 330 340 350
WSNELSPGKH LKTQLELNSV KSWMNDDFIT FSTTIKTGYL KNLSSQQSLP
360 370 380 390 400
VHICDKFQSG GPSDIRGFQT FGLGPRDLYD AVGGDAFVSY GLSVFSRLPW
410 420 430 440 450
KKVEKSNFRL HWFFNGGKLV NHDNTSLGNC IGQLSKEHST STGIGLVLRH
460 470 480
PMARFELNFT LPITAHENDL IRKGFQFGLG LAFL
Length:484
Mass (Da):54,406
Last modified:October 1, 1996 - v1
Checksum:i651A1FE08A1F1845
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z71302 Genomic DNA Translation: CAA95888.1
BK006947 Genomic DNA Translation: DAA10518.1
PIRiS62938
RefSeqiNP_014372.1, NM_001182865.1

Genome annotation databases

EnsemblFungiiYNL026W; YNL026W; YNL026W
GeneIDi855705
KEGGisce:YNL026W

Similar proteinsi

Entry informationi

Entry nameiSAM50_YEAST
AccessioniPrimary (citable) accession number: P53969
Secondary accession number(s): D6W1F2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: April 25, 2018
This is version 139 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health