Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P53969 (SAM50_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 103. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Sorting assembly machinery 50 kDa subunit
Alternative name(s):
TOB complex 55 kDa subunit
Gene names
Name:SAM50
Synonyms:TOB55
Ordered Locus Names:YNL026W
ORF Names:N2802
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length484 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Component of the mitochondrial outer membrane sorting assembly machinery (SAM or TOB) complex, which is required for the sorting of proteins with complicated topology, such as beta-barrel proteins, to the mitochondrial outer membrane after import by the TOM complex. Ref.3

Subunit structure

Component of the mitochondrial outer membrane sorting assembly machinery (SAM or TOB) complex, which at least consists of SAM35, SAM37 and SAM50. Associates with the mitochondrial inner membrane MINOS/MitOS complex By similarity. Ref.3 Ref.4 Ref.5

Subcellular location

Mitochondrion outer membrane; Multi-pass membrane protein By similarity Ref.3.

Domain

Its C-terminal part seems to contain many membrane-spanning sided beta-sheets, that have the potential to adopt a transmembrane beta-barrel type structure.

Sequence similarities

Belongs to the SAM50/omp85 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 484484Sorting assembly machinery 50 kDa subunit
PRO_0000215943

Sequences

Sequence LengthMass (Da)Tools
P53969 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: 651A1FE08A1F1845

FASTA48454,406
        10         20         30         40         50         60 
MTSSSGVDNE ISLDSPMPIF NESSTLKPIR VAGVVTTGTD HIDPSVLQAY LDDTIMKSIT 

        70         80         90        100        110        120 
LGQLVKNADV LNKRLCQHHI ALNAKQSFHF QGNTYISDEK ETHDVVPLME VVSQLDILPP 

       130        140        150        160        170        180 
KTFTAKTGTN FGNDNDAEAY LQFEKLIDKK YLKLPTRVNL EILRGTKIHS SFLFNSYSSL 

       190        200        210        220        230        240 
SPQSILNLKV FSQFYNWNTN KGLDIGQRGA RLSLRYEPLF LHKLLHNPHS NESPTLFHEW 

       250        260        270        280        290        300 
FLETCWRSTK ICSQGTSAPY MYSGTMLSQA GDQLRTILGH TFVLDKRDHI MCPTKGSMLK 

       310        320        330        340        350        360 
WSNELSPGKH LKTQLELNSV KSWMNDDFIT FSTTIKTGYL KNLSSQQSLP VHICDKFQSG 

       370        380        390        400        410        420 
GPSDIRGFQT FGLGPRDLYD AVGGDAFVSY GLSVFSRLPW KKVEKSNFRL HWFFNGGKLV 

       430        440        450        460        470        480 
NHDNTSLGNC IGQLSKEHST STGIGLVLRH PMARFELNFT LPITAHENDL IRKGFQFGLG 


LAFL 

« Hide

References

« Hide 'large scale' references
[1]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications."
Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J. expand/collapse author list , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.
Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[3]"An essential role of Sam50 in the protein sorting and assembly machinery of the mitochondrial outer membrane."
Kozjak V., Wiedemann N., Milenkovic D., Lohaus C., Meyer H.E., Guiard B., Meisinger C., Pfanner N.
J. Biol. Chem. 278:48520-48523(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY, FUNCTION, SUBCELLULAR LOCATION, IDENTIFICATION IN THE SAM COMPLEX.
[4]"Evolutionary conservation of biogenesis of beta-barrel membrane proteins."
Paschen S.A., Waizenegger T., Stan T., Preuss M., Cyrklaff M., Hell K., Rapaport D., Neupert W.
Nature 426:862-866(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION IN THE SAM COMPLEX.
[5]"Assembly of the TOB complex of mitochondria."
Habib S.J., Waizenegger T., Lech M., Neupert W., Rapaport D.
J. Biol. Chem. 280:6434-6440(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION IN THE SAM COMPLEX.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z71302 Genomic DNA. Translation: CAA95888.1.
BK006947 Genomic DNA. Translation: DAA10518.1.
PIRS62938.
RefSeqNP_014372.1. NM_001182865.1.

3D structure databases

ProteinModelPortalP53969.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid35800. 12 interactions.
IntActP53969. 13 interactions.
MINTMINT-4791160.
STRING4932.YNL026W.

Protein family/group databases

TCDB1.B.33.3.1. the outer membrane protein insertion porin (bam complex) (ompip) family.

Proteomic databases

PaxDbP53969.
PeptideAtlasP53969.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYNL026W; YNL026W; YNL026W.
GeneID855705.
KEGGsce:YNL026W.

Organism-specific databases

CYGDYNL026w.
SGDS000004971. SAM50.

Phylogenomic databases

eggNOGCOG4775.
GeneTreeENSGT00390000011355.
HOGENOMHOG000170308.
KOK07277.
OrthoDBEOG7W9S4R.

Enzyme and pathway databases

BioCycYEAST:G3O-33064-MONOMER.
ReactomeREACT_118590. Mitochondrial Protein Import (yeast).
REACT_85873. Metabolism of proteins.

Gene expression databases

GenevestigatorP53969.

Family and domain databases

InterProIPR000184. Bac_surfAg_D15.
[Graphical view]
PfamPF01103. Bac_surface_Ag. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio980043.
PROP53969.

Entry information

Entry nameSAM50_YEAST
AccessionPrimary (citable) accession number: P53969
Secondary accession number(s): D6W1F2
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: April 16, 2014
This is version 103 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XIV

Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Uncharacterized protein families (UPF)

List of uncharacterized protein family (UPF) entries

SIMILARITY comments

Index of protein domains and families