P53969 (SAM50_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Sorting assembly machinery 50 kDa subunit

Alternative name(s):
TOB complex 55 kDa subunit

Gene names

Name:	SAM50
Synonyms:	TOB55
Ordered Locus Names:	YNL026W
ORF Names:	N2802

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	484 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Component of the mitochondrial outer membrane sorting assembly machinery (SAM or TOB) complex, which is required for the sorting of proteins with complicated topology, such as beta-barrel proteins, to the mitochondrial outer membrane after import by the TOM complex. Ref.3
Subunit structure	Component of the mitochondrial outer membrane sorting assembly machinery (SAM or TOB) complex, which at least consists of SAM35, SAM37 and SAM50. Associates with the mitochondrial inner membrane MINOS/MitOS complex By similarity. Ref.3 Ref.4 Ref.5
Subcellular location	Mitochondrion outer membrane; Multi-pass membrane protein By similarity Ref.3.
Domain	Its C-terminal part seems to contain many membrane-spanning sided beta-sheets, that have the potential to adopt a transmembrane beta-barrel type structure.
Sequence similarities	Belongs to the SAM50/omp85 family.

Ontologies

Keywords
Biological process	Protein transport Transport
Cellular component	Membrane Mitochondrion Mitochondrion outer membrane
Domain	Transmembrane Transmembrane beta strand
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	protein complex assembly Inferred from mutant phenotype Ref.3Ref.4. Source: SGD protein import into mitochondrial outer membrane Inferred from mutant phenotype Ref.3PubMed 18039934. Source: SGD
Cellular_component	integral to mitochondrial outer membrane Inferred from direct assay PubMed 14699090. Source: SGD mitochondrial sorting and assembly machinery complex Inferred from direct assay Ref.3. Source: SGD
Molecular_function	protein channel activity Inferred from direct assay Ref.4. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
SAM35	P14693	3	EBI-28646,EBI-24602

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 484	484	Sorting assembly machinery 50 kDa subunit		PRO_0000215943

Sequences

	Sequence		Length	Mass (Da)
	P53969 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 651A1FE08A1F1845	FASTA	484	54,406
	10 20 30 40 50 60 MTSSSGVDNE ISLDSPMPIF NESSTLKPIR VAGVVTTGTD HIDPSVLQAY LDDTIMKSIT 70 80 90 100 110 120 LGQLVKNADV LNKRLCQHHI ALNAKQSFHF QGNTYISDEK ETHDVVPLME VVSQLDILPP 130 140 150 160 170 180 KTFTAKTGTN FGNDNDAEAY LQFEKLIDKK YLKLPTRVNL EILRGTKIHS SFLFNSYSSL 190 200 210 220 230 240 SPQSILNLKV FSQFYNWNTN KGLDIGQRGA RLSLRYEPLF LHKLLHNPHS NESPTLFHEW 250 260 270 280 290 300 FLETCWRSTK ICSQGTSAPY MYSGTMLSQA GDQLRTILGH TFVLDKRDHI MCPTKGSMLK 310 320 330 340 350 360 WSNELSPGKH LKTQLELNSV KSWMNDDFIT FSTTIKTGYL KNLSSQQSLP VHICDKFQSG 370 380 390 400 410 420 GPSDIRGFQT FGLGPRDLYD AVGGDAFVSY GLSVFSRLPW KKVEKSNFRL HWFFNGGKLV 430 440 450 460 470 480 NHDNTSLGNC IGQLSKEHST STGIGLVLRH PMARFELNFT LPITAHENDL IRKGFQFGLG LAFL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications." Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J. , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J. Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"An essential role of Sam50 in the protein sorting and assembly machinery of the mitochondrial outer membrane." Kozjak V., Wiedemann N., Milenkovic D., Lohaus C., Meyer H.E., Guiard B., Meisinger C., Pfanner N. J. Biol. Chem. 278:48520-48523(2003) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY, FUNCTION, SUBCELLULAR LOCATION, IDENTIFICATION IN THE SAM COMPLEX.
[4]	"Evolutionary conservation of biogenesis of beta-barrel membrane proteins." Paschen S.A., Waizenegger T., Stan T., Preuss M., Cyrklaff M., Hell K., Rapaport D., Neupert W. Nature 426:862-866(2003) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION IN THE SAM COMPLEX.
[5]	"Assembly of the TOB complex of mitochondria." Habib S.J., Waizenegger T., Lech M., Neupert W., Rapaport D. J. Biol. Chem. 280:6434-6440(2005) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION IN THE SAM COMPLEX.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z71302 Genomic DNA. Translation: CAA95888.1. BK006947 Genomic DNA. Translation: DAA10518.1.
PIR	S62938.
RefSeq	NP_014372.1. NM_001182865.1.
3D structure databases
ProteinModelPortal	P53969.
ModBase	Search...
Protein-protein interaction databases
IntAct	P53969. 3 interactions.
MINT	MINT-4791160.
STRING	4932.YNL026W.
Protein family/group databases
TCDB	1.B.33.3.1. outer membrane protein insertion porin (Bam Complex) (OmpIP) family.
Proteomic databases
PaxDb	P53969.
PeptideAtlas	P53969.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YNL026W; YNL026W; YNL026W.
GeneID	855705.
KEGG	sce:YNL026W.
Organism-specific databases
CYGD	YNL026w.
SGD	S000004971. SAM50.
Phylogenomic databases
eggNOG	COG4775.
GeneTree	ENSGT00390000011355.
HOGENOM	HOG000170308.
OrthoDB	EOG45XC52.
Enzyme and pathway databases
BioCyc	YEAST:G3O-33064-MONOMER.
Reactome	REACT_118590. Mitochondrial Protein Import (yeast). REACT_85873. Metabolism of proteins.
Gene expression databases
ArrayExpress	P53969.
Genevestigator	P53969.
GermOnline	YNL026W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR000184. Bac_surfAg_D15. [Graphical view]
Pfam	PF01103. Bac_surface_Ag. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	980043.

Entry information

Entry name

SAM50_YEAST

Accession

Primary (citable) accession number: P53969
Secondary accession number(s): D6W1F2

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	May 29, 2013
This is version 96 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program