P53966 (KTR5_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable mannosyltransferase KTR5
EC=2.4.1.-

Gene names

Name:	KTR5
Ordered Locus Names:	YNL029C
ORF Names:	N2755

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	522 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Possible glycosyltransferase.
Catalytic activity	Transfers an alpha-D-mannosyl residue from GDP-mannose into lipid-linked oligosaccharide, forming an alpha-(1->2)-D-mannosyl-D-mannose linkage.
Subcellular location	Membrane; Single-pass type II membrane protein Probable.
Miscellaneous	Present with 450 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the glycosyltransferase 15 family.

Ontologies

Keywords
Cellular component	Membrane
Domain	Signal-anchor Transmembrane Transmembrane helix
Molecular function	Glycosyltransferase Transferase
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	fungal-type cell wall organization Inferred from mutant phenotype Ref.3. Source: SGD protein N-linked glycosylation Inferred from sequence or structural similarity PubMed 9878809. Source: SGD
Cellular_component	Golgi apparatus Inferred from sequence or structural similarity PubMed 9878809. Source: SGD integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	mannosyltransferase activity Inferred from sequence or structural similarity PubMed 9878809. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 522	522	Probable mannosyltransferase KTR5		PRO_0000208246
Regions
	Topological domain	1 – 16	16	Cytoplasmic Potential
	Transmembrane	17 – 37	21	Helical; Signal-anchor for type II membrane protein; Potential
	Topological domain	38 – 522	485	Lumenal Potential
	Region	38 – 82	45	Stem region By similarity
	Region	83 – 522	440	Catalytic By similarity
Sites
	Active site	363	1	Nucleophile Potential
Amino acid modifications
	Glycosylation	86	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	P53966 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 3F9D75D3F09D4110	FASTA	522	61,728
	10 20 30 40 50 60 MLLIRRTINA FLGCIHCNLT ATCILIAFVI TMYVVLVSEP ASVDGTMGNF LPFSKMDLAT 70 80 90 100 110 120 KRDRPFYSNC VNTQDYLLNP SYIKQNASFV MLTRNGELED VIKTINSIEE HFNQWFHYPY 130 140 150 160 170 180 VFLNDQPFEE DFKAKVRDVT VGALVEFGTI DEISWNFPSD VKDTFEFYNA IEDQGDRSIL 190 200 210 220 230 240 YGNLESYHKM CRFYSGLFYK HPLVQKYEWY WRLEPDVEFF CDITYDPFLE MLRTNKKYGF 250 260 270 280 290 300 TIIIPELYWT VPNLFRHTKS FISQKGVTLG SLWKLFTKDY DIFESDDPEL RDWINYDFQA 310 320 330 340 350 360 KAKISEKIAI EQLLKKGDDF QQINDDKEGI MNLIHKARSR KHIVEDKFFN EEYNLCHFWS 370 380 390 400 410 420 NFEIARLSVF DNDIYNSFFQ YLEKSGGFWK ERWGDAPVHS IGLSLTLDLD DVHYFRDIGY 430 440 450 460 470 480 RHSTIQHCPH NAMGNEEFSY LASDSKFKRK NAAYDEGREF GCGCRCRCPK KKREIEDSMG 490 500 510 520 FCVNIWVNLL NQQRGHERHV EALNGNEMEE HIREDYLRQF GN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications." Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J. , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J. Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Completion of the Saccharomyces cerevisiae genome sequence allows identification of KTR5, KTR6 and KTR7 and definition of the nine-membered KRE2/MNT1 mannosyltransferase gene family in this organism." Lussier M., Sdicu A.-M., Winnett E., Vo D.H., Sheraton J., Duesterhoeft A., Storms R.K., Bussey H. Yeast 13:267-274(1997) [PubMed] [Europe PMC] [Abstract] Cited for: CHARACTERIZATION.
[4]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z71305 Genomic DNA. Translation: CAA95891.1. BK006947 Genomic DNA. Translation: DAA10516.1.
PIR	S62941.
RefSeq	NP_014369.3. NM_001182868.3.
3D structure databases
ProteinModelPortal	P53966.
SMR	P53966. Positions 73-429.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-6646N.
IntAct	P53966. 2 interactions.
MINT	MINT-614539.
STRING	4932.YNL029C.
Protein family/group databases
CAZy	GT15. Glycosyltransferase Family 15.
Proteomic databases
PaxDb	P53966.
PeptideAtlas	P53966.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YNL029C; YNL029C; YNL029C.
GeneID	855703.
KEGG	sce:YNL029C.
Organism-specific databases
CYGD	YNL029c.
SGD	S000004974. KTR5.
Phylogenomic databases
eggNOG	COG5020.
GeneTree	ENSGT00390000000831.
HOGENOM	HOG000160667.
KO	K03854.
OMA	MERHFNQ.
OrthoDB	EOG4F4WKD.
Enzyme and pathway databases
BioCyc	YEAST:G3O-33066-MONOMER.
Gene expression databases
Genevestigator	P53966.
GermOnline	YNL029C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR002685. Glyco_trans_15. [Graphical view]
Pfam	PF01793. Glyco_transf_15. 1 hit. [Graphical view]
PIRSF	PIRSF018153. Glyco_trans_15. 1 hit.
ProtoNet	Search...
Other
NextBio	980037.

Entry information

Entry name

KTR5_YEAST

Accession

Primary (citable) accession number: P53966
Secondary accession number(s): D6W1F0

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	May 29, 2013
This is version 106 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program