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P53965

- SIW14_YEAST

UniProt

P53965 - SIW14_YEAST

Protein

Tyrosine-protein phosphatase SIW14

Gene

SIW14

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 107 (01 Oct 2014)
      Sequence version 1 (01 Oct 1996)
      Previous versions | rss
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    Functioni

    Plays a role in actin filament organization and endocytosis.1 Publication

    Catalytic activityi

    Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.PROSITE-ProRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei214 – 2141Phosphocysteine intermediatePROSITE-ProRule annotation

    GO - Molecular functioni

    1. protein binding Source: IntAct
    2. protein tyrosine phosphatase activity Source: SGD

    GO - Biological processi

    1. actin filament organization Source: SGD
    2. endocytosis Source: SGD
    3. peptidyl-tyrosine dephosphorylation Source: GOC
    4. protein dephosphorylation Source: SGD

    Keywords - Molecular functioni

    Hydrolase, Protein phosphatase

    Enzyme and pathway databases

    BioCyciYEAST:G3O-33069-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Tyrosine-protein phosphatase SIW14 (EC:3.1.3.48)
    Gene namesi
    Name:SIW14
    Ordered Locus Names:YNL032W
    ORF Names:N2746
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XIV

    Organism-specific databases

    CYGDiYNL032w.
    SGDiS000004977. SIW14.

    Subcellular locationi

    Cytoplasm 1 Publication

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 281281Tyrosine-protein phosphatase SIW14PRO_0000094922Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei94 – 941Phosphoserine2 Publications

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP53965.
    PaxDbiP53965.

    Expressioni

    Gene expression databases

    GenevestigatoriP53965.

    Interactioni

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    OCA1P509464EBI-28668,EBI-28814
    OCA2P539494EBI-28668,EBI-28725

    Protein-protein interaction databases

    BioGridi35795. 115 interactions.
    DIPiDIP-1990N.
    IntActiP53965. 21 interactions.
    MINTiMINT-384829.
    STRINGi4932.YNL032W.

    Structurei

    3D structure databases

    ProteinModelPortaliP53965.
    SMRiP53965. Positions 116-263.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG2365.
    GeneTreeiENSGT00530000066535.
    HOGENOMiHOG000188365.
    KOiK18045.
    OMAiPPENFSH.
    OrthoDBiEOG7Z0K6V.

    Family and domain databases

    Gene3Di3.90.190.10. 1 hit.
    InterProiIPR029021. Prot-tyrosine_phosphatase-like.
    IPR016130. Tyr_Pase_AS.
    IPR020428. Tyr_Pase_dualsp-Pase_euk.
    IPR004861. Tyr_Pase_SIW14-like.
    [Graphical view]
    PfamiPF03162. Y_phosphatase2. 1 hit.
    [Graphical view]
    PRINTSiPR01911. PFDSPHPHTASE.
    SUPFAMiSSF52799. SSF52799. 1 hit.
    PROSITEiPS00383. TYR_PHOSPHATASE_1. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P53965-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGLYQAKNDE GSDPKSSSKI DDLIENEAEI IRLIKEDGKL LIDNGDGRDI    50
    HNIIQEDKLL SVEFNEVLKR FHGEEKSDIP RKEFDEDEDD GYDSNEHHQK 100
    TIEVMNTLNH VINKEVIPPE NFSHVVGEIY RSSFPRQENF SFLHERLKLK 150
    SILVLIPEEY PQENLNFLKL TGIKLYQVGM SGNKEPFVNI PSHLLTKALE 200
    IVLNPANQPI LIHCNRGKHR TGCLIGCIRK LQNWSLTMIF DEYRRFAFPK 250
    ARALDQQFIE MYDDDEIKRI ASKNNWLPLQ W 281
    Length:281
    Mass (Da):32,755
    Last modified:October 1, 1996 - v1
    Checksum:i88BD8AFC620B4570
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z71308 Genomic DNA. Translation: CAA95895.1.
    BK006947 Genomic DNA. Translation: DAA10513.1.
    PIRiS62954.
    RefSeqiNP_014366.3. NM_001182871.3.

    Genome annotation databases

    EnsemblFungiiYNL032W; YNL032W; YNL032W.
    GeneIDi855699.
    KEGGisce:YNL032W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z71308 Genomic DNA. Translation: CAA95895.1 .
    BK006947 Genomic DNA. Translation: DAA10513.1 .
    PIRi S62954.
    RefSeqi NP_014366.3. NM_001182871.3.

    3D structure databases

    ProteinModelPortali P53965.
    SMRi P53965. Positions 116-263.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 35795. 115 interactions.
    DIPi DIP-1990N.
    IntActi P53965. 21 interactions.
    MINTi MINT-384829.
    STRINGi 4932.YNL032W.

    Proteomic databases

    MaxQBi P53965.
    PaxDbi P53965.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YNL032W ; YNL032W ; YNL032W .
    GeneIDi 855699.
    KEGGi sce:YNL032W.

    Organism-specific databases

    CYGDi YNL032w.
    SGDi S000004977. SIW14.

    Phylogenomic databases

    eggNOGi COG2365.
    GeneTreei ENSGT00530000066535.
    HOGENOMi HOG000188365.
    KOi K18045.
    OMAi PPENFSH.
    OrthoDBi EOG7Z0K6V.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-33069-MONOMER.

    Miscellaneous databases

    NextBioi 980028.

    Gene expression databases

    Genevestigatori P53965.

    Family and domain databases

    Gene3Di 3.90.190.10. 1 hit.
    InterProi IPR029021. Prot-tyrosine_phosphatase-like.
    IPR016130. Tyr_Pase_AS.
    IPR020428. Tyr_Pase_dualsp-Pase_euk.
    IPR004861. Tyr_Pase_SIW14-like.
    [Graphical view ]
    Pfami PF03162. Y_phosphatase2. 1 hit.
    [Graphical view ]
    PRINTSi PR01911. PFDSPHPHTASE.
    SUPFAMi SSF52799. SSF52799. 1 hit.
    PROSITEi PS00383. TYR_PHOSPHATASE_1. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications."
      Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J.
      , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.
      Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    3. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    4. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    5. "A synthetic lethal screen identifies a role for the cortical actin patch/endocytosis complex in the response to nutrient deprivation in Saccharomyces cerevisiae."
      Care A., Vousden K.A., Binley K.M., Radcliffe P., Trevethick J., Mannazzu I., Sudbery P.E.
      Genetics 166:707-719(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    6. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
      Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
      J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-94, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: ADR376.
    7. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-94, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiSIW14_YEAST
    AccessioniPrimary (citable) accession number: P53965
    Secondary accession number(s): D6W1E7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 107 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 4070 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome XIV
      Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

    External Data

    Dasty 3