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P53965 (SIW14_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 104. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Tyrosine-protein phosphatase SIW14

EC=3.1.3.48
Gene names
Name:SIW14
Ordered Locus Names:YNL032W
ORF Names:N2746
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length281 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Plays a role in actin filament organization and endocytosis. Ref.5

Catalytic activity

Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.

Subcellular location

Cytoplasm Ref.3.

Miscellaneous

Present with 4070 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the protein-tyrosine phosphatase family.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

OCA1P509464EBI-28668,EBI-28814
OCA2P539494EBI-28668,EBI-28725

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 281281Tyrosine-protein phosphatase SIW14
PRO_0000094922

Sites

Active site2141Phosphocysteine intermediate By similarity

Amino acid modifications

Modified residue941Phosphoserine Ref.6 Ref.7

Sequences

Sequence LengthMass (Da)Tools
P53965 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: 88BD8AFC620B4570

FASTA28132,755
        10         20         30         40         50         60 
MGLYQAKNDE GSDPKSSSKI DDLIENEAEI IRLIKEDGKL LIDNGDGRDI HNIIQEDKLL 

        70         80         90        100        110        120 
SVEFNEVLKR FHGEEKSDIP RKEFDEDEDD GYDSNEHHQK TIEVMNTLNH VINKEVIPPE 

       130        140        150        160        170        180 
NFSHVVGEIY RSSFPRQENF SFLHERLKLK SILVLIPEEY PQENLNFLKL TGIKLYQVGM 

       190        200        210        220        230        240 
SGNKEPFVNI PSHLLTKALE IVLNPANQPI LIHCNRGKHR TGCLIGCIRK LQNWSLTMIF 

       250        260        270        280 
DEYRRFAFPK ARALDQQFIE MYDDDEIKRI ASKNNWLPLQ W 

« Hide

References

« Hide 'large scale' references
[1]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications."
Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J. expand/collapse author list , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.
Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[3]"Global analysis of protein localization in budding yeast."
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.
Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[4]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[5]"A synthetic lethal screen identifies a role for the cortical actin patch/endocytosis complex in the response to nutrient deprivation in Saccharomyces cerevisiae."
Care A., Vousden K.A., Binley K.M., Radcliffe P., Trevethick J., Mannazzu I., Sudbery P.E.
Genetics 166:707-719(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[6]"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-94, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Strain: ADR376.
[7]"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-94, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z71308 Genomic DNA. Translation: CAA95895.1.
BK006947 Genomic DNA. Translation: DAA10513.1.
PIRS62954.
RefSeqNP_014366.3. NM_001182871.3.

3D structure databases

ProteinModelPortalP53965.
SMRP53965. Positions 116-263.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid35795. 115 interactions.
DIPDIP-1990N.
IntActP53965. 21 interactions.
MINTMINT-384829.
STRING4932.YNL032W.

Proteomic databases

PaxDbP53965.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYNL032W; YNL032W; YNL032W.
GeneID855699.
KEGGsce:YNL032W.

Organism-specific databases

CYGDYNL032w.
SGDS000004977. SIW14.

Phylogenomic databases

eggNOGCOG2365.
GeneTreeENSGT00530000066535.
HOGENOMHOG000188365.
KOK18045.
OMAPPENFSH.
OrthoDBEOG7Z0K6V.

Enzyme and pathway databases

BioCycYEAST:G3O-33069-MONOMER.

Gene expression databases

GenevestigatorP53965.

Family and domain databases

InterProIPR016130. Tyr_Pase_AS.
IPR020428. Tyr_Pase_dualsp-Pase_euk.
IPR004861. Tyr_Pase_SIW14-like.
[Graphical view]
PfamPF03162. Y_phosphatase2. 1 hit.
[Graphical view]
PRINTSPR01911. PFDSPHPHTASE.
PROSITEPS00383. TYR_PHOSPHATASE_1. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio980028.

Entry information

Entry nameSIW14_YEAST
AccessionPrimary (citable) accession number: P53965
Secondary accession number(s): D6W1E7
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: April 16, 2014
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XIV

Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families