ID   YND5_YEAST              Reviewed;         389 AA.
AC   P53962; D6W1E4;
DT   01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1996, sequence version 1.
DT   27-MAR-2024, entry version 168.
DE   RecName: Full=Uncharacterized WD repeat-containing protein YNL035C;
GN   OrderedLocusNames=YNL035C; ORFNames=N2730;
OS   Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX   NCBI_TaxID=559292;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=9169873;
RA   Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K.,
RA   Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K.,
RA   Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M.,
RA   Beinhauer J.D., Boskovic J., Buitrago M.J., Bussereau F., Coster F.,
RA   Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F.,
RA   Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C.,
RA   Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A.,
RA   Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H.,
RA   Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L.,
RA   Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R.,
RA   Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D.,
RA   Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A.,
RA   Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C.,
RA   Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F.,
RA   Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G.,
RA   Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M.,
RA   Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.;
RT   "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its
RT   evolutionary implications.";
RL   Nature 387:93-98(1997).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=24374639; DOI=10.1534/g3.113.008995;
RA   Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA   Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA   Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT   "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL   G3 (Bethesda) 4:389-398(2014).
RN   [3]
RP   LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX   PubMed=14562106; DOI=10.1038/nature02046;
RA   Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA   O'Shea E.K., Weissman J.S.;
RT   "Global analysis of protein expression in yeast.";
RL   Nature 425:737-741(2003).
RN   [4]
RP   PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-351, AND IDENTIFICATION BY
RP   MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   STRAIN=ADR376;
RX   PubMed=17330950; DOI=10.1021/pr060559j;
RA   Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J.,
RA   Elias J.E., Gygi S.P.;
RT   "Large-scale phosphorylation analysis of alpha-factor-arrested
RT   Saccharomyces cerevisiae.";
RL   J. Proteome Res. 6:1190-1197(2007).
RN   [5]
RP   PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-351, AND IDENTIFICATION BY
RP   MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX   PubMed=18407956; DOI=10.1074/mcp.m700468-mcp200;
RA   Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.;
RT   "A multidimensional chromatography technology for in-depth phosphoproteome
RT   analysis.";
RL   Mol. Cell. Proteomics 7:1389-1396(2008).
RN   [6]
RP   PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-351, AND IDENTIFICATION BY
RP   MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX   PubMed=19779198; DOI=10.1126/science.1172867;
RA   Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.;
RT   "Global analysis of Cdk1 substrate phosphorylation sites provides insights
RT   into evolution.";
RL   Science 325:1682-1686(2009).
CC   -!- MISCELLANEOUS: Present with 3970 molecules/cell in log phase SD medium.
CC       {ECO:0000269|PubMed:14562106}.
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DR   EMBL; Z71311; CAA95900.1; -; Genomic_DNA.
DR   EMBL; BK006947; DAA10510.1; -; Genomic_DNA.
DR   PIR; S62957; S62957.
DR   RefSeq; NP_014363.1; NM_001182874.1.
DR   AlphaFoldDB; P53962; -.
DR   SMR; P53962; -.
DR   BioGRID; 35791; 60.
DR   DIP; DIP-6372N; -.
DR   IntAct; P53962; 2.
DR   MINT; P53962; -.
DR   STRING; 4932.YNL035C; -.
DR   iPTMnet; P53962; -.
DR   MaxQB; P53962; -.
DR   PaxDb; 4932-YNL035C; -.
DR   PeptideAtlas; P53962; -.
DR   EnsemblFungi; YNL035C_mRNA; YNL035C; YNL035C.
DR   GeneID; 855695; -.
DR   KEGG; sce:YNL035C; -.
DR   AGR; SGD:S000004980; -.
DR   SGD; S000004980; YNL035C.
DR   VEuPathDB; FungiDB:YNL035C; -.
DR   eggNOG; KOG1188; Eukaryota.
DR   GeneTree; ENSGT00390000006996; -.
DR   HOGENOM; CLU_037323_3_1_1; -.
DR   InParanoid; P53962; -.
DR   OMA; SIHSCGW; -.
DR   OrthoDB; 2726956at2759; -.
DR   BioCyc; YEAST:G3O-33072-MONOMER; -.
DR   BioGRID-ORCS; 855695; 5 hits in 10 CRISPR screens.
DR   PRO; PR:P53962; -.
DR   Proteomes; UP000002311; Chromosome XIV.
DR   RNAct; P53962; Protein.
DR   GO; GO:0005829; C:cytosol; IDA:SGD.
DR   GO; GO:0005634; C:nucleus; IDA:SGD.
DR   Gene3D; 2.130.10.10; YVTN repeat-like/Quinoprotein amine dehydrogenase; 1.
DR   InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR   InterPro; IPR036322; WD40_repeat_dom_sf.
DR   InterPro; IPR001680; WD40_rpt.
DR   InterPro; IPR039328; WDR89.
DR   PANTHER; PTHR22889; UNCHARACTERIZED; 1.
DR   PANTHER; PTHR22889:SF0; WD REPEAT-CONTAINING PROTEIN 89; 1.
DR   Pfam; PF00400; WD40; 2.
DR   SMART; SM00320; WD40; 4.
DR   SUPFAM; SSF50978; WD40 repeat-like; 1.
DR   PROSITE; PS00678; WD_REPEATS_1; 1.
DR   PROSITE; PS50082; WD_REPEATS_2; 1.
DR   PROSITE; PS50294; WD_REPEATS_REGION; 1.
PE   1: Evidence at protein level;
KW   Phosphoprotein; Reference proteome; Repeat; WD repeat.
FT   CHAIN           1..389
FT                   /note="Uncharacterized WD repeat-containing protein
FT                   YNL035C"
FT                   /id="PRO_0000051485"
FT   REPEAT          11..53
FT                   /note="WD 1"
FT   REPEAT          146..186
FT                   /note="WD 2"
FT   REPEAT          289..330
FT                   /note="WD 3"
FT   REGION          361..389
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        371..389
FT                   /note="Basic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOD_RES         351
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0007744|PubMed:17330950,
FT                   ECO:0007744|PubMed:18407956, ECO:0007744|PubMed:19779198"
SQ   SEQUENCE   389 AA;  43848 MW;  DEE82AEA215E5239 CRC64;
     MASYSLVESN SFGSENWCLK LQPSYKHGLL TGLSNGEIRL LDWSTGKSVQ KIKASETAIN
     DMKVIGSDFS AGHLVSSASI DAVKVFDIRT NDRIAQIQNE ANSPFISLDS RHGLLACGTE
     LQGIDAAVYI YDIRKWDTPL RSLIDSHHDD VTCIKFHPSD VNILLSGSTD GYTNIYDLKQ
     DEEEDALHQV INYASIHSCG WLSPKRIFTL SHMETFAIHE LNDKSDELKE PQPLDFGDVR
     EIWNCDYVVD IYPGLIATGK TQENCGELCL LPFKDEKVDT ENGIVIPHAH GDEVVRDIFI
     PAQHSNMLYS CGEDGCVKIW ENKQGPLDIP ENFWDYSKKM NVLGDDDREG SINLDEPLII
     QKESVSTRPR KEKHKKAKKH SMKSRFKPY
//