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Protein

Putative alanyl-tRNA editing protein alaX

Gene

YNL040W

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

May function in trans to edit the amino acid moiety from incorrectly charged tRNA(Ala).Curated

Miscellaneous

Present with 1470 molecules/cell in log phase SD medium.1 Publication

Caution

Conflicting data shows that it is not able to edit the amino acid moiety from incorrectly charged Ser-tRNA(Ala) in trans (PubMed:14663147). Another paper shows that this protein can edit mischarged Ser-tRNA(Ala) but not Gly-tRNA(Ala) in trans (PubMed:20010690). Experiments are not well detailed in either paper.2 Publications

Cofactori

Zn2+CuratedNote: Binds 1 zinc ion per subunit.Curated

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi125ZincSequence analysis1
Metal bindingi129ZincSequence analysis1
Metal bindingi240ZincSequence analysis1
Metal bindingi244ZincSequence analysis1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processProtein biosynthesis
LigandMetal-binding, Zinc

Enzyme and pathway databases

BioCyciYEAST:G3O-33076-MONOMER
ReactomeiR-SCE-2162123 Synthesis of Prostaglandins (PG) and Thromboxanes (TX)
R-SCE-3371497 HSP90 chaperone cycle for steroid hormone receptors (SHR)
R-SCE-3371511 HSF1 activation
R-SCE-3371568 Attenuation phase
R-SCE-8939211 ESR-mediated signaling

Names & Taxonomyi

Protein namesi
Recommended name:
Putative alanyl-tRNA editing protein alaX
Short name:
AlaX
Short name:
AlaXp
Short name:
AlaXp-II
Alternative name(s):
Alanyl-tRNA deacylase alaX
Gene namesi
Ordered Locus Names:YNL040W
ORF Names:N2679
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIV

Organism-specific databases

EuPathDBiFungiDB:YNL040W
SGDiS000004985 YNL040W

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002034571 – 456Putative alanyl-tRNA editing protein alaXAdd BLAST456

Proteomic databases

MaxQBiP53960
PaxDbiP53960
PRIDEiP53960

Interactioni

Protein-protein interaction databases

BioGridi35784, 106 interactors
DIPiDIP-6332N
IntActiP53960, 2 interactors
MINTiP53960
STRINGi4932.YNL040W

Structurei

3D structure databases

ProteinModelPortaliP53960
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00510000046493
HOGENOMiHOG000230967
InParanoidiP53960
KOiK07050
OMAiRNPCCGT
OrthoDBiEOG092C1Q5K

Family and domain databases

InterProiView protein in InterPro
IPR018164 Ala-tRNA-synth_IIc_N
IPR018163 Thr/Ala-tRNA-synth_IIc_edit
IPR009000 Transl_B-barrel_sf
IPR012947 tRNA_SAD
PfamiView protein in Pfam
PF01411 tRNA-synt_2c, 1 hit
PF07973 tRNA_SAD, 1 hit
SMARTiView protein in SMART
SM00863 tRNA_SAD, 1 hit
SUPFAMiSSF50447 SSF50447, 1 hit
SSF55186 SSF55186, 1 hit

Sequencei

Sequence statusi: Complete.

P53960-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPTPMTPVKV GALACQRNSF LFDGFKTLVV SCEPTKNKKG EIEGYEIELQ
60 70 80 90 100
DTILFPEGGG QPSDSGFLKI VEGNRNSSKI EKILVSHVSR FGLHAKHHVN
110 120 130 140 150
DYIEPGTTVE VAVDEQKRMD YMQQHTGQHL LSAILERNYK VDTVSWSMGG
160 170 180 190 200
IITKKKPVLE PSDYFNYIEL NRKLTLDEIT NVSDEINQLI INFPQEIIVE
210 220 230 240 250
ERIGEETVDE VSTSKIPDDY DLSKGVLRTI HIGDIDSNPC CGTHLKCTSQ
260 270 280 290 300
IGSILILSNQ SAVRGSNSRL YFMCGKRVSL YAKSVNKILL DSKNLLSCSE
310 320 330 340 350
TQISEKITRQ TKQIQQLNKR EQYWIKRLAR TASEELMNTL KASGKKRAYF
360 370 380 390 400
MEEEYGTLEL LLQIHKEVSN FLKDDTEGYE IILCGYERQT NTGSLLILSE
410 420 430 440 450
SGEKIANLAA NLGSILQNLK GGGGKKGGKW QGKITSISNA EFAALSDYLS

HDFASC
Length:456
Mass (Da):50,964
Last modified:October 1, 1996 - v1
Checksum:i5F636037485D1707
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z71316 Genomic DNA Translation: CAA95907.1
BK006947 Genomic DNA Translation: DAA10505.1
PIRiS62962
RefSeqiNP_014358.3, NM_001182879.3

Genome annotation databases

EnsemblFungiiYNL040W; YNL040W; YNL040W
GeneIDi855688
KEGGisce:YNL040W

Similar proteinsi

Entry informationi

Entry nameiYNE0_YEAST
AccessioniPrimary (citable) accession number: P53960
Secondary accession number(s): D6W1D9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: May 23, 2018
This is version 128 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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