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P53960

- YNE0_YEAST

UniProt

P53960 - YNE0_YEAST

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Protein

Putative alanyl-tRNA editing protein alaX

Gene

YNL040W

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

May function in trans to edit the amino acid moiety from incorrectly charged tRNA(Ala).Curated

Cofactori

Zn2+CuratedNote: Binds 1 zinc ion per subunit.Curated

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi125 – 1251ZincSequence Analysis
Metal bindingi129 – 1291ZincSequence Analysis
Metal bindingi240 – 2401ZincSequence Analysis
Metal bindingi244 – 2441ZincSequence Analysis

GO - Molecular functioni

  1. alanine-tRNA ligase activity Source: InterPro
  2. ATP binding Source: InterPro
  3. metal ion binding Source: UniProtKB-KW
  4. Ser-tRNA(Ala) hydrolase activity Source: UniProtKB

GO - Biological processi

  1. alanyl-tRNA aminoacylation Source: InterPro
  2. regulation of translational fidelity Source: GOC
Complete GO annotation...

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciYEAST:G3O-33076-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Putative alanyl-tRNA editing protein alaX
Short name:
AlaX
Short name:
AlaXp
Short name:
AlaXp-II
Alternative name(s):
Alanyl-tRNA deacylase alaX
Gene namesi
Ordered Locus Names:YNL040W
ORF Names:N2679
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XIV

Organism-specific databases

CYGDiYNL040w.
SGDiS000004985. YNL040W.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 456456Putative alanyl-tRNA editing protein alaXPRO_0000203457Add
BLAST

Proteomic databases

MaxQBiP53960.
PaxDbiP53960.
PeptideAtlasiP53960.

Expressioni

Gene expression databases

GenevestigatoriP53960.

Interactioni

Protein-protein interaction databases

BioGridi35784. 26 interactions.
DIPiDIP-6332N.
IntActiP53960. 1 interaction.
MINTiMINT-4499377.
STRINGi4932.YNL040W.

Structurei

3D structure databases

ProteinModelPortaliP53960.
SMRiP53960. Positions 42-317.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG2872.
GeneTreeiENSGT00510000046493.
HOGENOMiHOG000230967.
InParanoidiP53960.
KOiK07050.
OMAiYIELPEK.
OrthoDBiEOG73FQWN.

Family and domain databases

InterProiIPR018164. Ala-tRNA-synth_IIc_N.
IPR018163. Thr/Ala-tRNA-synth_IIc_edit.
IPR009000. Transl_B-barrel.
IPR012947. tRNA_SAD.
[Graphical view]
PfamiPF01411. tRNA-synt_2c. 1 hit.
PF07973. tRNA_SAD. 1 hit.
[Graphical view]
SMARTiSM00863. tRNA_SAD. 1 hit.
[Graphical view]
SUPFAMiSSF50447. SSF50447. 1 hit.
SSF55186. SSF55186. 1 hit.

Sequencei

Sequence statusi: Complete.

P53960-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MPTPMTPVKV GALACQRNSF LFDGFKTLVV SCEPTKNKKG EIEGYEIELQ
60 70 80 90 100
DTILFPEGGG QPSDSGFLKI VEGNRNSSKI EKILVSHVSR FGLHAKHHVN
110 120 130 140 150
DYIEPGTTVE VAVDEQKRMD YMQQHTGQHL LSAILERNYK VDTVSWSMGG
160 170 180 190 200
IITKKKPVLE PSDYFNYIEL NRKLTLDEIT NVSDEINQLI INFPQEIIVE
210 220 230 240 250
ERIGEETVDE VSTSKIPDDY DLSKGVLRTI HIGDIDSNPC CGTHLKCTSQ
260 270 280 290 300
IGSILILSNQ SAVRGSNSRL YFMCGKRVSL YAKSVNKILL DSKNLLSCSE
310 320 330 340 350
TQISEKITRQ TKQIQQLNKR EQYWIKRLAR TASEELMNTL KASGKKRAYF
360 370 380 390 400
MEEEYGTLEL LLQIHKEVSN FLKDDTEGYE IILCGYERQT NTGSLLILSE
410 420 430 440 450
SGEKIANLAA NLGSILQNLK GGGGKKGGKW QGKITSISNA EFAALSDYLS

HDFASC
Length:456
Mass (Da):50,964
Last modified:October 1, 1996 - v1
Checksum:i5F636037485D1707
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z71316 Genomic DNA. Translation: CAA95907.1.
BK006947 Genomic DNA. Translation: DAA10505.1.
PIRiS62962.
RefSeqiNP_014358.3. NM_001182879.3.

Genome annotation databases

EnsemblFungiiYNL040W; YNL040W; YNL040W.
GeneIDi855688.
KEGGisce:YNL040W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z71316 Genomic DNA. Translation: CAA95907.1 .
BK006947 Genomic DNA. Translation: DAA10505.1 .
PIRi S62962.
RefSeqi NP_014358.3. NM_001182879.3.

3D structure databases

ProteinModelPortali P53960.
SMRi P53960. Positions 42-317.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 35784. 26 interactions.
DIPi DIP-6332N.
IntActi P53960. 1 interaction.
MINTi MINT-4499377.
STRINGi 4932.YNL040W.

Proteomic databases

MaxQBi P53960.
PaxDbi P53960.
PeptideAtlasi P53960.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YNL040W ; YNL040W ; YNL040W .
GeneIDi 855688.
KEGGi sce:YNL040W.

Organism-specific databases

CYGDi YNL040w.
SGDi S000004985. YNL040W.

Phylogenomic databases

eggNOGi COG2872.
GeneTreei ENSGT00510000046493.
HOGENOMi HOG000230967.
InParanoidi P53960.
KOi K07050.
OMAi YIELPEK.
OrthoDBi EOG73FQWN.

Enzyme and pathway databases

BioCyci YEAST:G3O-33076-MONOMER.

Miscellaneous databases

NextBioi 979998.
PROi P53960.

Gene expression databases

Genevestigatori P53960.

Family and domain databases

InterProi IPR018164. Ala-tRNA-synth_IIc_N.
IPR018163. Thr/Ala-tRNA-synth_IIc_edit.
IPR009000. Transl_B-barrel.
IPR012947. tRNA_SAD.
[Graphical view ]
Pfami PF01411. tRNA-synt_2c. 1 hit.
PF07973. tRNA_SAD. 1 hit.
[Graphical view ]
SMARTi SM00863. tRNA_SAD. 1 hit.
[Graphical view ]
SUPFAMi SSF50447. SSF50447. 1 hit.
SSF55186. SSF55186. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications."
    Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J.
    , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.
    Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  4. Cited for: LACK OF EDITING ACTIVITY ON MISCHARGED TRNA(ALA).
  5. "Paradox of mistranslation of serine for alanine caused by AlaRS recognition dilemma."
    Guo M., Chong Y.E., Shapiro R., Beebe K., Yang X.L., Schimmel P.
    Nature 462:808-812(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION AS TRNA(ALA) EDITING PROTEIN.
  6. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiYNE0_YEAST
AccessioniPrimary (citable) accession number: P53960
Secondary accession number(s): D6W1D9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: November 26, 2014
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1470 molecules/cell in log phase SD medium.1 Publication

Caution

Conflicting data shows that it is not able to edit the amino acid moiety from incorrectly charged Ser-tRNA(Ala) in trans (PubMed:14663147). Another paper shows that this protein can edit mischarged Ser-tRNA(Ala) but not Gly-tRNA(Ala) in trans (PubMed:20010690). Experiments are not well detailed in either paper.2 Publications

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XIV
    Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

External Data

Dasty 3