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P53960

- YNE0_YEAST

UniProt

P53960 - YNE0_YEAST

Protein

Putative alanyl-tRNA editing protein alaX

Gene

YNL040W

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 97 (01 Oct 2014)
      Sequence version 1 (01 Oct 1996)
      Previous versions | rss
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    Functioni

    May function in trans to edit the amino acid moiety from incorrectly charged tRNA(Ala).Curated

    Cofactori

    Binds 1 zinc ion per subunit.Curated

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi125 – 1251ZincSequence Analysis
    Metal bindingi129 – 1291ZincSequence Analysis
    Metal bindingi240 – 2401ZincSequence Analysis
    Metal bindingi244 – 2441ZincSequence Analysis

    GO - Molecular functioni

    1. alanine-tRNA ligase activity Source: InterPro
    2. ATP binding Source: InterPro
    3. metal ion binding Source: UniProtKB-KW
    4. Ser-tRNA(Ala) hydrolase activity Source: UniProtKB

    GO - Biological processi

    1. alanyl-tRNA aminoacylation Source: InterPro
    2. regulation of translational fidelity Source: GOC

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    Metal-binding, Zinc

    Enzyme and pathway databases

    BioCyciYEAST:G3O-33076-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Putative alanyl-tRNA editing protein alaX
    Short name:
    AlaX
    Short name:
    AlaXp
    Short name:
    AlaXp-II
    Alternative name(s):
    Alanyl-tRNA deacylase alaX
    Gene namesi
    Ordered Locus Names:YNL040W
    ORF Names:N2679
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XIV

    Organism-specific databases

    CYGDiYNL040w.
    SGDiS000004985. YNL040W.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 456456Putative alanyl-tRNA editing protein alaXPRO_0000203457Add
    BLAST

    Proteomic databases

    MaxQBiP53960.
    PaxDbiP53960.
    PeptideAtlasiP53960.

    Expressioni

    Gene expression databases

    GenevestigatoriP53960.

    Interactioni

    Protein-protein interaction databases

    BioGridi35784. 26 interactions.
    DIPiDIP-6332N.
    IntActiP53960. 1 interaction.
    MINTiMINT-4499377.
    STRINGi4932.YNL040W.

    Structurei

    3D structure databases

    ProteinModelPortaliP53960.
    SMRiP53960. Positions 49-276.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG2872.
    GeneTreeiENSGT00510000046493.
    HOGENOMiHOG000230967.
    KOiK07050.
    OMAiYIELPEK.
    OrthoDBiEOG73FQWN.

    Family and domain databases

    InterProiIPR018164. Ala-tRNA-synth_IIc_N.
    IPR018163. Thr/Ala-tRNA-synth_IIc_edit.
    IPR009000. Transl_B-barrel.
    IPR012947. tRNA_SAD.
    [Graphical view]
    PfamiPF01411. tRNA-synt_2c. 1 hit.
    PF07973. tRNA_SAD. 1 hit.
    [Graphical view]
    SMARTiSM00863. tRNA_SAD. 1 hit.
    [Graphical view]
    SUPFAMiSSF50447. SSF50447. 1 hit.
    SSF55186. SSF55186. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P53960-1 [UniParc]FASTAAdd to Basket

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    MPTPMTPVKV GALACQRNSF LFDGFKTLVV SCEPTKNKKG EIEGYEIELQ    50
    DTILFPEGGG QPSDSGFLKI VEGNRNSSKI EKILVSHVSR FGLHAKHHVN 100
    DYIEPGTTVE VAVDEQKRMD YMQQHTGQHL LSAILERNYK VDTVSWSMGG 150
    IITKKKPVLE PSDYFNYIEL NRKLTLDEIT NVSDEINQLI INFPQEIIVE 200
    ERIGEETVDE VSTSKIPDDY DLSKGVLRTI HIGDIDSNPC CGTHLKCTSQ 250
    IGSILILSNQ SAVRGSNSRL YFMCGKRVSL YAKSVNKILL DSKNLLSCSE 300
    TQISEKITRQ TKQIQQLNKR EQYWIKRLAR TASEELMNTL KASGKKRAYF 350
    MEEEYGTLEL LLQIHKEVSN FLKDDTEGYE IILCGYERQT NTGSLLILSE 400
    SGEKIANLAA NLGSILQNLK GGGGKKGGKW QGKITSISNA EFAALSDYLS 450
    HDFASC 456
    Length:456
    Mass (Da):50,964
    Last modified:October 1, 1996 - v1
    Checksum:i5F636037485D1707
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z71316 Genomic DNA. Translation: CAA95907.1.
    BK006947 Genomic DNA. Translation: DAA10505.1.
    PIRiS62962.
    RefSeqiNP_014358.3. NM_001182879.3.

    Genome annotation databases

    EnsemblFungiiYNL040W; YNL040W; YNL040W.
    GeneIDi855688.
    KEGGisce:YNL040W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z71316 Genomic DNA. Translation: CAA95907.1 .
    BK006947 Genomic DNA. Translation: DAA10505.1 .
    PIRi S62962.
    RefSeqi NP_014358.3. NM_001182879.3.

    3D structure databases

    ProteinModelPortali P53960.
    SMRi P53960. Positions 49-276.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 35784. 26 interactions.
    DIPi DIP-6332N.
    IntActi P53960. 1 interaction.
    MINTi MINT-4499377.
    STRINGi 4932.YNL040W.

    Proteomic databases

    MaxQBi P53960.
    PaxDbi P53960.
    PeptideAtlasi P53960.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YNL040W ; YNL040W ; YNL040W .
    GeneIDi 855688.
    KEGGi sce:YNL040W.

    Organism-specific databases

    CYGDi YNL040w.
    SGDi S000004985. YNL040W.

    Phylogenomic databases

    eggNOGi COG2872.
    GeneTreei ENSGT00510000046493.
    HOGENOMi HOG000230967.
    KOi K07050.
    OMAi YIELPEK.
    OrthoDBi EOG73FQWN.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-33076-MONOMER.

    Miscellaneous databases

    NextBioi 979998.
    PROi P53960.

    Gene expression databases

    Genevestigatori P53960.

    Family and domain databases

    InterProi IPR018164. Ala-tRNA-synth_IIc_N.
    IPR018163. Thr/Ala-tRNA-synth_IIc_edit.
    IPR009000. Transl_B-barrel.
    IPR012947. tRNA_SAD.
    [Graphical view ]
    Pfami PF01411. tRNA-synt_2c. 1 hit.
    PF07973. tRNA_SAD. 1 hit.
    [Graphical view ]
    SMARTi SM00863. tRNA_SAD. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50447. SSF50447. 1 hit.
    SSF55186. SSF55186. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications."
      Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J.
      , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.
      Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    3. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    4. Cited for: LACK OF EDITING ACTIVITY ON MISCHARGED TRNA(ALA).
    5. "Paradox of mistranslation of serine for alanine caused by AlaRS recognition dilemma."
      Guo M., Chong Y.E., Shapiro R., Beebe K., Yang X.L., Schimmel P.
      Nature 462:808-812(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION AS TRNA(ALA) EDITING PROTEIN.
    6. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiYNE0_YEAST
    AccessioniPrimary (citable) accession number: P53960
    Secondary accession number(s): D6W1D9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 97 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 1470 molecules/cell in log phase SD medium.1 Publication

    Caution

    Conflicting data shows that it is not able to edit the amino acid moiety from incorrectly charged Ser-tRNA(Ala) in trans (PubMed:14663147). Another paper shows that this protein can edit mischarged Ser-tRNA(Ala) but not Gly-tRNA(Ala) in trans (PubMed:20010690). Experiments are not well detailed in either paper.2 Publications

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome XIV
      Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

    External Data

    Dasty 3