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Protein

GDP-Man:Man(3)GlcNAc(2)-PP-Dol alpha-1,2-mannosyltransferase

Gene

ALG11

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Required for N-linked oligosaccharide assembly. Has a role in the last step of the synthesis of the Man5GlcNAc(2)-PP-dolichol core oligosaccharide on the cytoplasmic face of the endoplasmic reticulum.3 Publications

Catalytic activityi

2 GDP-alpha-D-mannose + D-Man-alpha-(1->3)-(D-Man-alpha-(1->6))-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol = 2 GDP + D-Man-alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-(D-Man-alpha-(1->6))-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol.2 Publications

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

GO - Molecular functioni

  • alpha-1,2-mannosyltransferase activity Source: SGD
  • GDP-Man:Man3GlcNAc2-PP-Dol alpha-1,2-mannosyltransferase activity Source: SGD

GO - Biological processi

  • dolichol-linked oligosaccharide biosynthetic process Source: Reactome
  • oligosaccharide-lipid intermediate biosynthetic process Source: SGD
  • protein glycosylation Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER3O-36.
YEAST:MONOMER3O-36.
BRENDAi2.4.1.131. 984.
ReactomeiR-SCE-446193. Biosynthesis of the N-glycan precursor (dolichol lipid-linked oligosaccharide, LLO) and transfer to a nascent protein.
UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT4. Glycosyltransferase Family 4.

Names & Taxonomyi

Protein namesi
Recommended name:
GDP-Man:Man(3)GlcNAc(2)-PP-Dol alpha-1,2-mannosyltransferase (EC:2.4.1.131)
Alternative name(s):
Alpha-1,2-mannosyltransferase ALG11
Asparagine-linked glycosylation protein 11
Glycolipid 2-alpha-mannosyltransferase
Gene namesi
Name:ALG11
Ordered Locus Names:YNL048W
ORF Names:N2510, YNL2510W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIV

Organism-specific databases

EuPathDBiFungiDB:YNL048W.
SGDiS000004993. ALG11.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 20CytoplasmicSequence analysisAdd BLAST20
Transmembranei21 – 41Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST21
Topological domaini42 – 548LumenalSequence analysisAdd BLAST507

GO - Cellular componenti

  • endoplasmic reticulum membrane Source: SGD
  • integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi84G → A or P: No effect. 1 Publication1
Mutagenesisi85G → A: No effect. 1 Publication1
Mutagenesisi85G → P: No activity. 1 Publication1
Mutagenesisi87G → A: No effect. 1 Publication1
Mutagenesisi87G → P: No activity. 1 Publication1
Mutagenesisi319K → A: Almost no activity. 1 Publication1
Mutagenesisi405E → A: Almost no activity. 2 Publications1
Mutagenesisi406H → A: Almost no activity. 1 Publication1
Mutagenesisi407F → A: Impaired activity. 1 Publication1
Mutagenesisi413E → A: No effect. 2 Publications1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000802801 – 548GDP-Man:Man(3)GlcNAc(2)-PP-Dol alpha-1,2-mannosyltransferaseAdd BLAST548

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi262N-linked (GlcNAc...)Sequence analysis1
Glycosylationi393N-linked (GlcNAc...)Sequence analysis1
Glycosylationi480N-linked (GlcNAc...)Sequence analysis1
Glycosylationi490N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiP53954.
PRIDEiP53954.

Interactioni

Subunit structurei

Interacts with ALG1.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
ALG1P166612EBI-2497,EBI-2206309

Protein-protein interaction databases

BioGridi35776. 19 interactors.
DIPiDIP-7438N.
IntActiP53954. 4 interactors.
MINTiMINT-1356407.

Structurei

3D structure databases

DisProtiDP00618.
ProteinModelPortaliP53954.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi84 – 87Poly-Gly4
Compositional biasi543 – 546Poly-Glu4

Sequence similaritiesi

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00550000075118.
HOGENOMiHOG000209670.
InParanoidiP53954.
KOiK03844.
OMAiYGFFHPY.
OrthoDBiEOG092C21XK.

Family and domain databases

InterProiIPR031814. ALG11_N.
IPR001296. Glyco_trans_1.
[Graphical view]
PfamiPF15924. ALG11_N. 1 hit.
PF00534. Glycos_transf_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P53954-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGSAWTNYNF EEVKSHFGFK KYVVSSLVLV YGLIKVLTWI FRQWVYSSLN
60 70 80 90 100
PFSKKSSLLN RAVASCGEKN VKVFGFFHPY CNAGGGGEKV LWKAVDITLR
110 120 130 140 150
KDAKNVIVIY SGDFVNGENV TPENILNNVK AKFDYDLDSD RIFFISLKLR
160 170 180 190 200
YLVDSSTWKH FTLIGQAIGS MILAFESIIQ CPPDIWIDTM GYPFSYPIIA
210 220 230 240 250
RFLRRIPIVT YTHYPIMSKD MLNKLFKMPK KGIKVYGKIL YWKVFMLIYQ
260 270 280 290 300
SIGSKIDIVI TNSTWTNNHI KQIWQSNTCK IIYPPCSTEK LVDWKQKFGT
310 320 330 340 350
AKGERLNQAI VLAQFRPEKR HKLIIESFAT FLKNLPDSVS PIKLIMAGST
360 370 380 390 400
RSKQDENYVK SLQDWSENVL KIPKHLISFE KNLPFDKIEI LLNKSTFGVN
410 420 430 440 450
AMWNEHFGIA VVEYMASGLI PIVHASAGPL LDIVTPWDAN GNIGKAPPQW
460 470 480 490 500
ELQKKYFAKL EDDGETTGFF FKEPSDPDYN TTKDPLRYPN LSDLFLQITK
510 520 530 540
LDYDCLRVMG ARNQQYSLYK FSDLKFDKDW ENFVLNPICK LLEEEERG
Length:548
Mass (Da):63,143
Last modified:October 1, 1996 - v1
Checksum:i2A3AD59924C762CC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U12141 Genomic DNA. Translation: AAA99664.1.
U62941 Genomic DNA. Translation: AAB49937.1.
X94547 Genomic DNA. Translation: CAA64234.1.
Z71324 Genomic DNA. Translation: CAA95916.1.
BK006947 Genomic DNA. Translation: DAA10497.1.
PIRiS61096.
RefSeqiNP_014350.1. NM_001182887.1.

Genome annotation databases

EnsemblFungiiYNL048W; YNL048W; YNL048W.
GeneIDi855679.
KEGGisce:YNL048W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U12141 Genomic DNA. Translation: AAA99664.1.
U62941 Genomic DNA. Translation: AAB49937.1.
X94547 Genomic DNA. Translation: CAA64234.1.
Z71324 Genomic DNA. Translation: CAA95916.1.
BK006947 Genomic DNA. Translation: DAA10497.1.
PIRiS61096.
RefSeqiNP_014350.1. NM_001182887.1.

3D structure databases

DisProtiDP00618.
ProteinModelPortaliP53954.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi35776. 19 interactors.
DIPiDIP-7438N.
IntActiP53954. 4 interactors.
MINTiMINT-1356407.

Protein family/group databases

CAZyiGT4. Glycosyltransferase Family 4.

Proteomic databases

MaxQBiP53954.
PRIDEiP53954.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYNL048W; YNL048W; YNL048W.
GeneIDi855679.
KEGGisce:YNL048W.

Organism-specific databases

EuPathDBiFungiDB:YNL048W.
SGDiS000004993. ALG11.

Phylogenomic databases

GeneTreeiENSGT00550000075118.
HOGENOMiHOG000209670.
InParanoidiP53954.
KOiK03844.
OMAiYGFFHPY.
OrthoDBiEOG092C21XK.

Enzyme and pathway databases

UniPathwayiUPA00378.
BioCyciMetaCyc:MONOMER3O-36.
YEAST:MONOMER3O-36.
BRENDAi2.4.1.131. 984.
ReactomeiR-SCE-446193. Biosynthesis of the N-glycan precursor (dolichol lipid-linked oligosaccharide, LLO) and transfer to a nascent protein.

Miscellaneous databases

PROiP53954.

Family and domain databases

InterProiIPR031814. ALG11_N.
IPR001296. Glyco_trans_1.
[Graphical view]
PfamiPF15924. ALG11_N. 1 hit.
PF00534. Glycos_transf_1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiALG11_YEAST
AccessioniPrimary (citable) accession number: P53954
Secondary accession number(s): D6W1D1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: November 2, 2016
This is version 131 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 3140 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XIV
    Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.