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P53954

- ALG11_YEAST

UniProt

P53954 - ALG11_YEAST

Protein

GDP-Man:Man(3)GlcNAc(2)-PP-Dol alpha-1,2-mannosyltransferase

Gene

ALG11

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 112 (01 Oct 2014)
      Sequence version 1 (01 Oct 1996)
      Previous versions | rss
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    Functioni

    Required for N-linked oligosaccharide assembly. Has a role in the last step of the synthesis of the Man5GlcNAc(2)-PP-dolichol core oligosaccharide on the cytoplasmic face of the endoplasmic reticulum.3 Publications

    Catalytic activityi

    2 GDP-alpha-D-mannose + D-Man-alpha-(1->3)-(D-Man-alpha-(1->6))-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol = 2 GDP + D-Man-alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-(D-Man-alpha-(1->6))-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol.2 Publications

    Pathwayi

    GO - Molecular functioni

    1. alpha-1,2-mannosyltransferase activity Source: SGD
    2. GDP-Man:Man3GlcNAc2-PP-Dol alpha-1,2-mannosyltransferase activity Source: UniProtKB-EC
    3. protein binding Source: IntAct

    GO - Biological processi

    1. mannosylation Source: GOC
    2. oligosaccharide-lipid intermediate biosynthetic process Source: SGD
    3. protein glycosylation Source: SGD

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-7187.
    YEAST:MONOMER3O-36.
    UniPathwayiUPA00378.

    Protein family/group databases

    CAZyiGT4. Glycosyltransferase Family 4.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    GDP-Man:Man(3)GlcNAc(2)-PP-Dol alpha-1,2-mannosyltransferase (EC:2.4.1.131)
    Alternative name(s):
    Alpha-1,2-mannosyltransferase ALG11
    Asparagine-linked glycosylation protein 11
    Glycolipid 2-alpha-mannosyltransferase
    Gene namesi
    Name:ALG11
    Ordered Locus Names:YNL048W
    ORF Names:N2510, YNL2510W
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XIV

    Organism-specific databases

    CYGDiYNL048w.
    SGDiS000004993. ALG11.

    Subcellular locationi

    Endoplasmic reticulum membrane 1 Publication; Single-pass type II membrane protein 1 Publication

    GO - Cellular componenti

    1. endoplasmic reticulum membrane Source: SGD
    2. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi84 – 841G → A or P: No effect. 1 Publication
    Mutagenesisi85 – 851G → A: No effect. 1 Publication
    Mutagenesisi85 – 851G → P: No activity. 1 Publication
    Mutagenesisi87 – 871G → A: No effect. 1 Publication
    Mutagenesisi87 – 871G → P: No activity. 1 Publication
    Mutagenesisi319 – 3191K → A: Almost no activity. 1 Publication
    Mutagenesisi405 – 4051E → A: Almost no activity. 2 Publications
    Mutagenesisi406 – 4061H → A: Almost no activity. 1 Publication
    Mutagenesisi407 – 4071F → A: Impaired activity. 1 Publication
    Mutagenesisi413 – 4131E → A: No effect. 2 Publications

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 548548GDP-Man:Man(3)GlcNAc(2)-PP-Dol alpha-1,2-mannosyltransferasePRO_0000080280Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi262 – 2621N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi393 – 3931N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi480 – 4801N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi490 – 4901N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    MaxQBiP53954.
    PaxDbiP53954.

    Expressioni

    Gene expression databases

    GenevestigatoriP53954.

    Interactioni

    Subunit structurei

    Interacts with ALG1.1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    ALG1P166612EBI-2497,EBI-2206309

    Protein-protein interaction databases

    BioGridi35776. 20 interactions.
    DIPiDIP-7438N.
    IntActiP53954. 4 interactions.
    MINTiMINT-1356407.
    STRINGi4932.YNL048W.

    Structurei

    3D structure databases

    DisProtiDP00618.
    ProteinModelPortaliP53954.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 2020CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini42 – 548507LumenalSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei21 – 4121Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi84 – 874Poly-Gly
    Compositional biasi543 – 5464Poly-Glu

    Sequence similaritiesi

    Keywords - Domaini

    Signal-anchor, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG0438.
    GeneTreeiENSGT00550000075118.
    HOGENOMiHOG000209670.
    KOiK03844.
    OMAiHPYANNG.
    OrthoDBiEOG7673KQ.

    Family and domain databases

    InterProiIPR001296. Glyco_trans_1.
    [Graphical view]
    PfamiPF00534. Glycos_transf_1. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P53954-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGSAWTNYNF EEVKSHFGFK KYVVSSLVLV YGLIKVLTWI FRQWVYSSLN    50
    PFSKKSSLLN RAVASCGEKN VKVFGFFHPY CNAGGGGEKV LWKAVDITLR 100
    KDAKNVIVIY SGDFVNGENV TPENILNNVK AKFDYDLDSD RIFFISLKLR 150
    YLVDSSTWKH FTLIGQAIGS MILAFESIIQ CPPDIWIDTM GYPFSYPIIA 200
    RFLRRIPIVT YTHYPIMSKD MLNKLFKMPK KGIKVYGKIL YWKVFMLIYQ 250
    SIGSKIDIVI TNSTWTNNHI KQIWQSNTCK IIYPPCSTEK LVDWKQKFGT 300
    AKGERLNQAI VLAQFRPEKR HKLIIESFAT FLKNLPDSVS PIKLIMAGST 350
    RSKQDENYVK SLQDWSENVL KIPKHLISFE KNLPFDKIEI LLNKSTFGVN 400
    AMWNEHFGIA VVEYMASGLI PIVHASAGPL LDIVTPWDAN GNIGKAPPQW 450
    ELQKKYFAKL EDDGETTGFF FKEPSDPDYN TTKDPLRYPN LSDLFLQITK 500
    LDYDCLRVMG ARNQQYSLYK FSDLKFDKDW ENFVLNPICK LLEEEERG 548
    Length:548
    Mass (Da):63,143
    Last modified:October 1, 1996 - v1
    Checksum:i2A3AD59924C762CC
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U12141 Genomic DNA. Translation: AAA99664.1.
    U62941 Genomic DNA. Translation: AAB49937.1.
    X94547 Genomic DNA. Translation: CAA64234.1.
    Z71324 Genomic DNA. Translation: CAA95916.1.
    BK006947 Genomic DNA. Translation: DAA10497.1.
    PIRiS61096.
    RefSeqiNP_014350.1. NM_001182887.1.

    Genome annotation databases

    EnsemblFungiiYNL048W; YNL048W; YNL048W.
    GeneIDi855679.
    KEGGisce:YNL048W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U12141 Genomic DNA. Translation: AAA99664.1 .
    U62941 Genomic DNA. Translation: AAB49937.1 .
    X94547 Genomic DNA. Translation: CAA64234.1 .
    Z71324 Genomic DNA. Translation: CAA95916.1 .
    BK006947 Genomic DNA. Translation: DAA10497.1 .
    PIRi S61096.
    RefSeqi NP_014350.1. NM_001182887.1.

    3D structure databases

    DisProti DP00618.
    ProteinModelPortali P53954.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 35776. 20 interactions.
    DIPi DIP-7438N.
    IntActi P53954. 4 interactions.
    MINTi MINT-1356407.
    STRINGi 4932.YNL048W.

    Protein family/group databases

    CAZyi GT4. Glycosyltransferase Family 4.

    Proteomic databases

    MaxQBi P53954.
    PaxDbi P53954.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YNL048W ; YNL048W ; YNL048W .
    GeneIDi 855679.
    KEGGi sce:YNL048W.

    Organism-specific databases

    CYGDi YNL048w.
    SGDi S000004993. ALG11.

    Phylogenomic databases

    eggNOGi COG0438.
    GeneTreei ENSGT00550000075118.
    HOGENOMi HOG000209670.
    KOi K03844.
    OMAi HPYANNG.
    OrthoDBi EOG7673KQ.

    Enzyme and pathway databases

    UniPathwayi UPA00378 .
    BioCyci MetaCyc:MONOMER-7187.
    YEAST:MONOMER3O-36.

    Miscellaneous databases

    NextBioi 979975.
    PROi P53954.

    Gene expression databases

    Genevestigatori P53954.

    Family and domain databases

    InterProi IPR001296. Glyco_trans_1.
    [Graphical view ]
    Pfami PF00534. Glycos_transf_1. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The yeast ALG11 gene specifies addition of the terminal alpha 1,2-Man to the Man5GlcNAc2-PP-dolichol N-glycosylation intermediate formed on the cytosolic side of the endoplasmic reticulum."
      Cipollo J.F., Trimble R.B., Chi J.H., Yan Q., Dean N.
      J. Biol. Chem. 276:21828-21840(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBCELLULAR LOCATION.
    2. "The sequence of a 44 420 bp fragment located on the left arm of chromosome XIV from Saccharomyces cerevisiae."
      Bergez P., Doignon F., Crouzet M.
      Yeast 11:967-974(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: S288c / FY1676.
    3. "The sequence of 12.8 kb from the left arm of chromosome XIV reveals a sigma element, a pro-tRNA and six complete open reading frames, one of which encodes a protein similar to the human leukotriene A4 hydrolase."
      Nasr F., Becam A.-M., Herbert C.J.
      Yeast 12:493-499(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 96604 / S288c / FY1679.
    4. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications."
      Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J.
      , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.
      Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    5. "In vitro evidence for the dual function of Alg2 and Alg11: essential mannosyltransferases in N-linked glycoprotein biosynthesis."
      O'Reilly M.K., Zhang G., Imperiali B.
      Biochemistry 45:9593-9603(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, CATALYTIC ACTIVITY, SUBSTRATE SPECIFICITY, MUTAGENESIS OF GLU-405 AND GLU-413.
    6. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    8. "Physical interactions between the Alg1, Alg2, and Alg11 mannosyltransferases of the endoplasmic reticulum."
      Gao X.-D., Nishikawa A., Dean N.
      Glycobiology 14:559-570(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH ALG1.
    9. "Biochemical characterization, membrane association and identification of amino acids essential for the function of Alg11 from Saccharomyces cerevisiae, an alpha1,2-mannosyltransferase catalysing two sequential glycosylation steps in the formation of the lipid-linked core oligosaccharide."
      Absmanner B., Schmeiser V., Kaempf M., Lehle L.
      Biochem. J. 426:205-217(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, CATALYTIC ACTIVITY, MUTAGENESIS OF GLY-84; GLY-85; GLY-87; LYS-319; GLU-405; HIS-406; PHE-407 AND GLU-413, BIOPHYSICOCHEMICAL PROPERTIES.

    Entry informationi

    Entry nameiALG11_YEAST
    AccessioniPrimary (citable) accession number: P53954
    Secondary accession number(s): D6W1D1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 112 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 3140 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome XIV
      Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

    External Data

    Dasty 3