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P53954

- ALG11_YEAST

UniProt

P53954 - ALG11_YEAST

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Protein

GDP-Man:Man(3)GlcNAc(2)-PP-Dol alpha-1,2-mannosyltransferase

Gene
ALG11, YNL048W, N2510, YNL2510W
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Required for N-linked oligosaccharide assembly. Has a role in the last step of the synthesis of the Man5GlcNAc(2)-PP-dolichol core oligosaccharide on the cytoplasmic face of the endoplasmic reticulum.3 Publications

Catalytic activityi

2 GDP-alpha-D-mannose + D-Man-alpha-(1->3)-(D-Man-alpha-(1->6))-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol = 2 GDP + D-Man-alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-(D-Man-alpha-(1->6))-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol.2 Publications

Pathwayi

GO - Molecular functioni

  1. alpha-1,2-mannosyltransferase activity Source: SGD
  2. GDP-Man:Man3GlcNAc2-PP-Dol alpha-1,2-mannosyltransferase activity Source: UniProtKB-EC
  3. protein binding Source: IntAct

GO - Biological processi

  1. mannosylation Source: GOC
  2. oligosaccharide-lipid intermediate biosynthetic process Source: SGD
  3. protein glycosylation Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-7187.
YEAST:MONOMER3O-36.
UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT4. Glycosyltransferase Family 4.

Names & Taxonomyi

Protein namesi
Recommended name:
GDP-Man:Man(3)GlcNAc(2)-PP-Dol alpha-1,2-mannosyltransferase (EC:2.4.1.131)
Alternative name(s):
Alpha-1,2-mannosyltransferase ALG11
Asparagine-linked glycosylation protein 11
Glycolipid 2-alpha-mannosyltransferase
Gene namesi
Name:ALG11
Ordered Locus Names:YNL048W
ORF Names:N2510, YNL2510W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XIV

Organism-specific databases

CYGDiYNL048w.
SGDiS000004993. ALG11.

Subcellular locationi

Endoplasmic reticulum membrane; Single-pass type II membrane protein 1 Publication

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 2020Cytoplasmic Reviewed predictionAdd
BLAST
Transmembranei21 – 4121Helical; Signal-anchor for type II membrane protein; Reviewed predictionAdd
BLAST
Topological domaini42 – 548507Lumenal Reviewed predictionAdd
BLAST

GO - Cellular componenti

  1. endoplasmic reticulum membrane Source: SGD
  2. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi84 – 841G → A or P: No effect. 1 Publication
Mutagenesisi85 – 851G → A: No effect. 1 Publication
Mutagenesisi85 – 851G → P: No activity. 1 Publication
Mutagenesisi87 – 871G → A: No effect. 1 Publication
Mutagenesisi87 – 871G → P: No activity. 1 Publication
Mutagenesisi319 – 3191K → A: Almost no activity. 1 Publication
Mutagenesisi405 – 4051E → A: Almost no activity. 2 Publications
Mutagenesisi406 – 4061H → A: Almost no activity. 1 Publication
Mutagenesisi407 – 4071F → A: Impaired activity. 1 Publication
Mutagenesisi413 – 4131E → A: No effect. 2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 548548GDP-Man:Man(3)GlcNAc(2)-PP-Dol alpha-1,2-mannosyltransferasePRO_0000080280Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi262 – 2621N-linked (GlcNAc...) Reviewed prediction
Glycosylationi393 – 3931N-linked (GlcNAc...) Reviewed prediction
Glycosylationi480 – 4801N-linked (GlcNAc...) Reviewed prediction
Glycosylationi490 – 4901N-linked (GlcNAc...) Reviewed prediction

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiP53954.
PaxDbiP53954.

Expressioni

Gene expression databases

GenevestigatoriP53954.

Interactioni

Subunit structurei

Interacts with ALG1.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
ALG1P166612EBI-2497,EBI-2206309

Protein-protein interaction databases

BioGridi35776. 20 interactions.
DIPiDIP-7438N.
IntActiP53954. 4 interactions.
MINTiMINT-1356407.
STRINGi4932.YNL048W.

Structurei

3D structure databases

DisProtiDP00618.
ProteinModelPortaliP53954.

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi84 – 874Poly-Gly
Compositional biasi543 – 5464Poly-Glu

Sequence similaritiesi

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0438.
GeneTreeiENSGT00550000075118.
HOGENOMiHOG000209670.
KOiK03844.
OMAiHPYANNG.
OrthoDBiEOG7673KQ.

Family and domain databases

InterProiIPR001296. Glyco_trans_1.
[Graphical view]
PfamiPF00534. Glycos_transf_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P53954-1 [UniParc]FASTAAdd to Basket

« Hide

MGSAWTNYNF EEVKSHFGFK KYVVSSLVLV YGLIKVLTWI FRQWVYSSLN    50
PFSKKSSLLN RAVASCGEKN VKVFGFFHPY CNAGGGGEKV LWKAVDITLR 100
KDAKNVIVIY SGDFVNGENV TPENILNNVK AKFDYDLDSD RIFFISLKLR 150
YLVDSSTWKH FTLIGQAIGS MILAFESIIQ CPPDIWIDTM GYPFSYPIIA 200
RFLRRIPIVT YTHYPIMSKD MLNKLFKMPK KGIKVYGKIL YWKVFMLIYQ 250
SIGSKIDIVI TNSTWTNNHI KQIWQSNTCK IIYPPCSTEK LVDWKQKFGT 300
AKGERLNQAI VLAQFRPEKR HKLIIESFAT FLKNLPDSVS PIKLIMAGST 350
RSKQDENYVK SLQDWSENVL KIPKHLISFE KNLPFDKIEI LLNKSTFGVN 400
AMWNEHFGIA VVEYMASGLI PIVHASAGPL LDIVTPWDAN GNIGKAPPQW 450
ELQKKYFAKL EDDGETTGFF FKEPSDPDYN TTKDPLRYPN LSDLFLQITK 500
LDYDCLRVMG ARNQQYSLYK FSDLKFDKDW ENFVLNPICK LLEEEERG 548
Length:548
Mass (Da):63,143
Last modified:October 1, 1996 - v1
Checksum:i2A3AD59924C762CC
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U12141 Genomic DNA. Translation: AAA99664.1.
U62941 Genomic DNA. Translation: AAB49937.1.
X94547 Genomic DNA. Translation: CAA64234.1.
Z71324 Genomic DNA. Translation: CAA95916.1.
BK006947 Genomic DNA. Translation: DAA10497.1.
PIRiS61096.
RefSeqiNP_014350.1. NM_001182887.1.

Genome annotation databases

EnsemblFungiiYNL048W; YNL048W; YNL048W.
GeneIDi855679.
KEGGisce:YNL048W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U12141 Genomic DNA. Translation: AAA99664.1 .
U62941 Genomic DNA. Translation: AAB49937.1 .
X94547 Genomic DNA. Translation: CAA64234.1 .
Z71324 Genomic DNA. Translation: CAA95916.1 .
BK006947 Genomic DNA. Translation: DAA10497.1 .
PIRi S61096.
RefSeqi NP_014350.1. NM_001182887.1.

3D structure databases

DisProti DP00618.
ProteinModelPortali P53954.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 35776. 20 interactions.
DIPi DIP-7438N.
IntActi P53954. 4 interactions.
MINTi MINT-1356407.
STRINGi 4932.YNL048W.

Protein family/group databases

CAZyi GT4. Glycosyltransferase Family 4.

Proteomic databases

MaxQBi P53954.
PaxDbi P53954.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YNL048W ; YNL048W ; YNL048W .
GeneIDi 855679.
KEGGi sce:YNL048W.

Organism-specific databases

CYGDi YNL048w.
SGDi S000004993. ALG11.

Phylogenomic databases

eggNOGi COG0438.
GeneTreei ENSGT00550000075118.
HOGENOMi HOG000209670.
KOi K03844.
OMAi HPYANNG.
OrthoDBi EOG7673KQ.

Enzyme and pathway databases

UniPathwayi UPA00378 .
BioCyci MetaCyc:MONOMER-7187.
YEAST:MONOMER3O-36.

Miscellaneous databases

NextBioi 979975.
PROi P53954.

Gene expression databases

Genevestigatori P53954.

Family and domain databases

InterProi IPR001296. Glyco_trans_1.
[Graphical view ]
Pfami PF00534. Glycos_transf_1. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The yeast ALG11 gene specifies addition of the terminal alpha 1,2-Man to the Man5GlcNAc2-PP-dolichol N-glycosylation intermediate formed on the cytosolic side of the endoplasmic reticulum."
    Cipollo J.F., Trimble R.B., Chi J.H., Yan Q., Dean N.
    J. Biol. Chem. 276:21828-21840(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBCELLULAR LOCATION.
  2. "The sequence of a 44 420 bp fragment located on the left arm of chromosome XIV from Saccharomyces cerevisiae."
    Bergez P., Doignon F., Crouzet M.
    Yeast 11:967-974(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: S288c / FY1676.
  3. "The sequence of 12.8 kb from the left arm of chromosome XIV reveals a sigma element, a pro-tRNA and six complete open reading frames, one of which encodes a protein similar to the human leukotriene A4 hydrolase."
    Nasr F., Becam A.-M., Herbert C.J.
    Yeast 12:493-499(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 96604 / S288c / FY1679.
  4. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications."
    Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J.
    , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.
    Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  5. "In vitro evidence for the dual function of Alg2 and Alg11: essential mannosyltransferases in N-linked glycoprotein biosynthesis."
    O'Reilly M.K., Zhang G., Imperiali B.
    Biochemistry 45:9593-9603(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, CATALYTIC ACTIVITY, SUBSTRATE SPECIFICITY, MUTAGENESIS OF GLU-405 AND GLU-413.
  6. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  8. "Physical interactions between the Alg1, Alg2, and Alg11 mannosyltransferases of the endoplasmic reticulum."
    Gao X.-D., Nishikawa A., Dean N.
    Glycobiology 14:559-570(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH ALG1.
  9. "Biochemical characterization, membrane association and identification of amino acids essential for the function of Alg11 from Saccharomyces cerevisiae, an alpha1,2-mannosyltransferase catalysing two sequential glycosylation steps in the formation of the lipid-linked core oligosaccharide."
    Absmanner B., Schmeiser V., Kaempf M., Lehle L.
    Biochem. J. 426:205-217(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, CATALYTIC ACTIVITY, MUTAGENESIS OF GLY-84; GLY-85; GLY-87; LYS-319; GLU-405; HIS-406; PHE-407 AND GLU-413, BIOPHYSICOCHEMICAL PROPERTIES.

Entry informationi

Entry nameiALG11_YEAST
AccessioniPrimary (citable) accession number: P53954
Secondary accession number(s): D6W1D1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: June 11, 2014
This is version 111 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 3140 molecules/cell in log phase SD medium.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XIV
    Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

External Data

Dasty 3

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