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P53954 (ALG11_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 111. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
GDP-Man:Man(3)GlcNAc(2)-PP-Dol alpha-1,2-mannosyltransferase

EC=2.4.1.131
Alternative name(s):
Alpha-1,2-mannosyltransferase ALG11
Asparagine-linked glycosylation protein 11
Glycolipid 2-alpha-mannosyltransferase
Gene names
Name:ALG11
Ordered Locus Names:YNL048W
ORF Names:N2510, YNL2510W
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length548 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Required for N-linked oligosaccharide assembly. Has a role in the last step of the synthesis of the Man5GlcNAc(2)-PP-dolichol core oligosaccharide on the cytoplasmic face of the endoplasmic reticulum. Ref.1 Ref.6 Ref.10

Catalytic activity

2 GDP-alpha-D-mannose + D-Man-alpha-(1->3)-(D-Man-alpha-(1->6))-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol = 2 GDP + D-Man-alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-(D-Man-alpha-(1->6))-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol. Ref.6 Ref.10

Pathway

Protein modification; protein glycosylation.

Subunit structure

Interacts with ALG1. Ref.9

Subcellular location

Endoplasmic reticulum membrane; Single-pass type II membrane protein Ref.1.

Miscellaneous

Present with 3140 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the glycosyltransferase group 1 family. Glycosyltransferase 4 subfamily.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

ALG1P166612EBI-2497,EBI-2206309

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 548548GDP-Man:Man(3)GlcNAc(2)-PP-Dol alpha-1,2-mannosyltransferase
PRO_0000080280

Regions

Topological domain1 – 2020Cytoplasmic Potential
Transmembrane21 – 4121Helical; Signal-anchor for type II membrane protein; Potential
Topological domain42 – 548507Lumenal Potential
Compositional bias84 – 874Poly-Gly
Compositional bias543 – 5464Poly-Glu

Amino acid modifications

Glycosylation2621N-linked (GlcNAc...) Potential
Glycosylation3931N-linked (GlcNAc...) Potential
Glycosylation4801N-linked (GlcNAc...) Potential
Glycosylation4901N-linked (GlcNAc...) Potential

Experimental info

Mutagenesis841G → A or P: No effect. Ref.10
Mutagenesis851G → A: No effect. Ref.10
Mutagenesis851G → P: No activity. Ref.10
Mutagenesis871G → A: No effect. Ref.10
Mutagenesis871G → P: No activity. Ref.10
Mutagenesis3191K → A: Almost no activity. Ref.10
Mutagenesis4051E → A: Almost no activity. Ref.6 Ref.10
Mutagenesis4061H → A: Almost no activity. Ref.10
Mutagenesis4071F → A: Impaired activity. Ref.10
Mutagenesis4131E → A: No effect. Ref.6 Ref.10

Sequences

Sequence LengthMass (Da)Tools
P53954 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: 2A3AD59924C762CC

FASTA54863,143
        10         20         30         40         50         60 
MGSAWTNYNF EEVKSHFGFK KYVVSSLVLV YGLIKVLTWI FRQWVYSSLN PFSKKSSLLN 

        70         80         90        100        110        120 
RAVASCGEKN VKVFGFFHPY CNAGGGGEKV LWKAVDITLR KDAKNVIVIY SGDFVNGENV 

       130        140        150        160        170        180 
TPENILNNVK AKFDYDLDSD RIFFISLKLR YLVDSSTWKH FTLIGQAIGS MILAFESIIQ 

       190        200        210        220        230        240 
CPPDIWIDTM GYPFSYPIIA RFLRRIPIVT YTHYPIMSKD MLNKLFKMPK KGIKVYGKIL 

       250        260        270        280        290        300 
YWKVFMLIYQ SIGSKIDIVI TNSTWTNNHI KQIWQSNTCK IIYPPCSTEK LVDWKQKFGT 

       310        320        330        340        350        360 
AKGERLNQAI VLAQFRPEKR HKLIIESFAT FLKNLPDSVS PIKLIMAGST RSKQDENYVK 

       370        380        390        400        410        420 
SLQDWSENVL KIPKHLISFE KNLPFDKIEI LLNKSTFGVN AMWNEHFGIA VVEYMASGLI 

       430        440        450        460        470        480 
PIVHASAGPL LDIVTPWDAN GNIGKAPPQW ELQKKYFAKL EDDGETTGFF FKEPSDPDYN 

       490        500        510        520        530        540 
TTKDPLRYPN LSDLFLQITK LDYDCLRVMG ARNQQYSLYK FSDLKFDKDW ENFVLNPICK 


LLEEEERG 

« Hide

References

« Hide 'large scale' references
[1]"The yeast ALG11 gene specifies addition of the terminal alpha 1,2-Man to the Man5GlcNAc2-PP-dolichol N-glycosylation intermediate formed on the cytosolic side of the endoplasmic reticulum."
Cipollo J.F., Trimble R.B., Chi J.H., Yan Q., Dean N.
J. Biol. Chem. 276:21828-21840(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBCELLULAR LOCATION.
[2]"The sequence of a 44 420 bp fragment located on the left arm of chromosome XIV from Saccharomyces cerevisiae."
Bergez P., Doignon F., Crouzet M.
Yeast 11:967-974(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: S288c / FY1676.
[3]Erratum
Bergez P., Doignon F., Crouzet M.
Yeast 12:297-297(1996) [PubMed] [Europe PMC] [Abstract]
[4]"The sequence of 12.8 kb from the left arm of chromosome XIV reveals a sigma element, a pro-tRNA and six complete open reading frames, one of which encodes a protein similar to the human leukotriene A4 hydrolase."
Nasr F., Becam A.-M., Herbert C.J.
Yeast 12:493-499(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 96604 / S288c / FY1679.
[5]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications."
Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J. expand/collapse author list , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.
Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[6]"In vitro evidence for the dual function of Alg2 and Alg11: essential mannosyltransferases in N-linked glycoprotein biosynthesis."
O'Reilly M.K., Zhang G., Imperiali B.
Biochemistry 45:9593-9603(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, CATALYTIC ACTIVITY, SUBSTRATE SPECIFICITY, MUTAGENESIS OF GLU-405 AND GLU-413.
[7]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[8]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[9]"Physical interactions between the Alg1, Alg2, and Alg11 mannosyltransferases of the endoplasmic reticulum."
Gao X.-D., Nishikawa A., Dean N.
Glycobiology 14:559-570(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH ALG1.
[10]"Biochemical characterization, membrane association and identification of amino acids essential for the function of Alg11 from Saccharomyces cerevisiae, an alpha1,2-mannosyltransferase catalysing two sequential glycosylation steps in the formation of the lipid-linked core oligosaccharide."
Absmanner B., Schmeiser V., Kaempf M., Lehle L.
Biochem. J. 426:205-217(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, CATALYTIC ACTIVITY, MUTAGENESIS OF GLY-84; GLY-85; GLY-87; LYS-319; GLU-405; HIS-406; PHE-407 AND GLU-413, BIOPHYSICOCHEMICAL PROPERTIES.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U12141 Genomic DNA. Translation: AAA99664.1.
U62941 Genomic DNA. Translation: AAB49937.1.
X94547 Genomic DNA. Translation: CAA64234.1.
Z71324 Genomic DNA. Translation: CAA95916.1.
BK006947 Genomic DNA. Translation: DAA10497.1.
PIRS61096.
RefSeqNP_014350.1. NM_001182887.1.

3D structure databases

DisProtDP00618.
ProteinModelPortalP53954.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid35776. 20 interactions.
DIPDIP-7438N.
IntActP53954. 4 interactions.
MINTMINT-1356407.
STRING4932.YNL048W.

Protein family/group databases

CAZyGT4. Glycosyltransferase Family 4.

Proteomic databases

MaxQBP53954.
PaxDbP53954.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYNL048W; YNL048W; YNL048W.
GeneID855679.
KEGGsce:YNL048W.

Organism-specific databases

CYGDYNL048w.
SGDS000004993. ALG11.

Phylogenomic databases

eggNOGCOG0438.
GeneTreeENSGT00550000075118.
HOGENOMHOG000209670.
KOK03844.
OMAHPYANNG.
OrthoDBEOG7673KQ.

Enzyme and pathway databases

BioCycMetaCyc:MONOMER-7187.
YEAST:MONOMER3O-36.
UniPathwayUPA00378.

Gene expression databases

GenevestigatorP53954.

Family and domain databases

InterProIPR001296. Glyco_trans_1.
[Graphical view]
PfamPF00534. Glycos_transf_1. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio979975.
PROP53954.

Entry information

Entry nameALG11_YEAST
AccessionPrimary (citable) accession number: P53954
Secondary accession number(s): D6W1D1
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: June 11, 2014
This is version 111 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XIV

Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways