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P53950 (VAC7_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 93. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Vacuolar segregation protein 7
Gene names
Name:VAC7
Ordered Locus Names:YNL054W
ORF Names:N2467, YNL2467W
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length1165 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

The PI(3,5)P2 regulatory complex regulates both the synthesis and turnover of phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2). Positively regulates FAB1 kinase activity. Major activator of FAB1 during hyperosmotic shock and can elevate levels of PtdIns(3,5)P2 in the absence of VAC14 and FIG4. Directly involved in vacuolar membrane scission. Required for normal vacuole acidification, inheritance and morphology. Ref.1 Ref.6 Ref.7 Ref.8

Subunit structure

Component of the PI(3,5)P2 regulatory complex, composed of ATG18, FIG4, FAB1, VAC14 and VAC7. VAC14 nucleates the assembly of the complex and serves as a scaffold. Ref.8

Subcellular location

Vacuole membrane; Single-pass type II membrane protein Ref.1 Ref.8.

Post-translational modification

N-glycosylated.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

VAC14Q067084EBI-28714,EBI-27189

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 11651165Vacuolar segregation protein 7
PRO_0000065757

Regions

Topological domain1 – 919919Cytoplasmic Potential
Transmembrane920 – 94021Helical; Signal-anchor for type II membrane protein; Potential
Topological domain941 – 1165225Vacuolar Potential
Compositional bias277 – 412136Asn-rich
Compositional bias469 – 48517Gln-rich
Compositional bias1085 – 111935Asp-rich

Amino acid modifications

Modified residue1641Phosphoserine Ref.11
Glycosylation10201N-linked (GlcNAc...) Potential
Glycosylation10991N-linked (GlcNAc...) Potential

Sequences

Sequence LengthMass (Da)Tools
P53950 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: 0414978AA3066CE7

FASTA1,165128,140
        10         20         30         40         50         60 
MTEEDRKLTV ETETVEAPVA NNLLLSNNSN VVAPNPSIPS ASTSTSPLHR EIVDDSVATA 

        70         80         90        100        110        120 
NTTSNVVQHN LPTIDNNLMD SDATSHNQDH WHSDINRAGT SMSTSDIPTD LHLEHIGSVS 

       130        140        150        160        170        180 
STNNNSNNAL INHNPLSSHL SNPSSSLRNK KSSLLVASNP AFASDVELSK KKPAVISNNM 

       190        200        210        220        230        240 
PTSNIALYQT ARSANIHGPS STSASKAFRK ASAFSNNTAP STSNNIGSNT PPAPLLPLPS 

       250        260        270        280        290        300 
LSQQNKPKII ERPTMHVTNS REILLGENLL DDTKAKNAPA NSTTHDNGPV ANDGLRIPNH 

       310        320        330        340        350        360 
SNADDNENNN KMKKNKNINS GKNERNDDTS KICTTSTKTA PSTAPLGSTD NTQALTASVS 

       370        380        390        400        410        420 
SSNADNHNNN KKKTSSNNNG NNSNSASNKT NADIKNSNAD LSASTSNNNA INDDSHESNS 

       430        440        450        460        470        480 
EKPTKADFFA ARLATAVGEN EISDSEETFV YESAANSTKN LIFPDSSSQQ QQQQQQPPKQ 

       490        500        510        520        530        540 
QQQQQNHGIT SKISAPLLNN NKKLLSRLKN SRHISTGAIL NNTIATISTN PNLNSNVMQN 

       550        560        570        580        590        600 
NNNLMSGHNH LDELSSIKQE PPHQLQQQQP PMDVQSVDSY TSDNPDSNVI AKSPDKRSSL 

       610        620        630        640        650        660 
VSLSKVSPHL LSSTSSNGNT ISCPNVATNS QELEPNNDIS TKKSLSNSTL RHSSANRNSN 

       670        680        690        700        710        720 
YGDNKRPLRT TVSKIFDSNP NGAPLRRYSG VPDHVNLEDY IEQPHNYPTM QNSVKKDEFY 

       730        740        750        760        770        780 
NSRNNKFPHG LNFYGDNNVI EEENNGDSSN VNRPQHTNLQ HEFIPEDNES DENDIHSMFY 

       790        800        810        820        830        840 
YNHKNDLETK PLISDYGEDE DVDDYDRPNA TFNSYYGSAS NTHELPLHGR MPSRSNNDYY 

       850        860        870        880        890        900 
DFMVGNNTGN NNQLNEYTPL RMKRGQRHLS RTNNSIMNGS IHMNGNDDVT HSNINNNDIV 

       910        920        930        940        950        960 
GYSPHNFYSR KSPFVKVKNF LYLAFVISSL LMTGFILGFL LATNKELQDV DVVVMDNVIS 

       970        980        990       1000       1010       1020 
SSDELIFDIT VSAFNPGFFS ISVSQVDLDI FAKSSYLKCD SNGDCTVMEQ ERKILQITTN 

      1030       1040       1050       1060       1070       1080 
LSLVEESANN DISGGNIETV LLGTAKKLET PLKFQGGAFN RNYDVSVSSV KLLSPGSREA 

      1090       1100       1110       1120       1130       1140 
KHENDDDDDD DGDDGDDENN TNERQYKSKP NARDDKEDDT KKWKLLIKHD YELIVRGSMK 

      1150       1160 
YEVPFFNTQK STAIQKDSMV HPGKK 

« Hide

References

« Hide 'large scale' references
[1]"Vac7p, a novel vacuolar protein, is required for normal vacuole inheritance and morphology."
Bonangelino C.J., Catlett N.L., Weisman L.S.
Mol. Cell. Biol. 17:6847-6858(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, TOPOLOGY, SUBCELLULAR LOCATION.
[2]"The sequence of a 44 420 bp fragment located on the left arm of chromosome XIV from Saccharomyces cerevisiae."
Bergez P., Doignon F., Crouzet M.
Yeast 11:967-974(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: S288c / FY1676.
[3]Erratum
Bergez P., Doignon F., Crouzet M.
Yeast 12:297-297(1996) [PubMed] [Europe PMC] [Abstract]
[4]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications."
Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J. expand/collapse author list , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.
Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[5]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[6]"Regulation of Fab1 phosphatidylinositol 3-phosphate 5-kinase pathway by Vac7 protein and Fig4, a polyphosphoinositide phosphatase family member."
Gary J.D., Sato T.K., Stefan C.J., Bonangelino C.J., Weisman L.S., Emr S.D.
Mol. Biol. Cell 13:1238-1251(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[7]"The Vac14p-Fig4p complex acts independently of Vac7p and couples PI3,5P2 synthesis and turnover."
Duex J.E., Tang F., Weisman L.S.
J. Cell Biol. 172:693-704(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[8]"VAC14 nucleates a protein complex essential for the acute interconversion of PI3P and PI(3,5)P(2) in yeast and mouse."
Jin N., Chow C.Y., Liu L., Zolov S.N., Bronson R., Davisson M., Petersen J.L., Zhang Y., Park S., Duex J.E., Goldowitz D., Meisler M.H., Weisman L.S.
EMBO J. 27:3221-3234(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION IN THE PI(3,5)P2 REGULATORY COMPLEX, FUNCTION, SUBCELLULAR LOCATION.
[9]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[10]"Global analysis of the glycoproteome in Saccharomyces cerevisiae reveals new roles for protein glycosylation in eukaryotes."
Kung L.A., Tao S.-C., Qian J., Smith M.G., Snyder M., Zhu H.
Mol. Syst. Biol. 5:308-308(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS].
[11]"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-164, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U12141 Genomic DNA. Translation: AAA99658.1.
Z71330 Genomic DNA. Translation: CAA95925.1.
BK006947 Genomic DNA. Translation: DAA10491.1.
PIRS62982.
RefSeqNP_014344.3. NM_001182893.3.

3D structure databases

ProteinModelPortalP53950.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid35770. 41 interactions.
DIPDIP-4368N.
IntActP53950. 4 interactions.
MINTMINT-531363.
STRING4932.YNL054W.

Proteomic databases

PaxDbP53950.
PRIDEP53950.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYNL054W; YNL054W; YNL054W.
GeneID855673.
KEGGsce:YNL054W.

Organism-specific databases

CYGDYNL054w.
SGDS000004999. VAC7.

Phylogenomic databases

eggNOGNOG42444.
OMAKANTENT.
OrthoDBEOG74N5R8.

Enzyme and pathway databases

BioCycMetaCyc:MONOMER-15184.
YEAST:G3O-33086-MONOMER.

Gene expression databases

GenevestigatorP53950.

Family and domain databases

InterProIPR024260. Vac7.
[Graphical view]
PfamPF12751. Vac7. 2 hits.
[Graphical view]
ProtoNetSearch...

Other

NextBio979957.

Entry information

Entry nameVAC7_YEAST
AccessionPrimary (citable) accession number: P53950
Secondary accession number(s): D6W1C5
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: April 16, 2014
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XIV

Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD