P53950 (VAC7_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Vacuolar segregation protein 7

Gene names

Name:	VAC7
Ordered Locus Names:	YNL054W
ORF Names:	N2467, YNL2467W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	1165 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	The PI(3,5)P2 regulatory complex regulates both the synthesis and turnover of phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2). Positively regulates FAB1 kinase activity. Major activator of FAB1 during hyperosmotic shock and can elevate levels of PtdIns(3,5)P2 in the absence of VAC14 and FIG4. Directly involved in vacuolar membrane scission. Required for normal vacuole acidification, inheritance and morphology. Ref.1 Ref.6 Ref.7 Ref.9
Subunit structure	Component of the PI(3,5)P2 regulatory complex, composed of ATG18, FIG4, FAB1, VAC14 and VAC7. VAC14 nucleates the assembly of the complex and serves as a scaffold. Ref.9
Subcellular location	Vacuole membrane; Single-pass type II membrane protein Ref.1 Ref.9.
Post-translational modification	N-glycosylated.

Ontologies

Keywords
Cellular component	Membrane Vacuole
Domain	Signal-anchor Transmembrane Transmembrane helix
PTM	Glycoprotein Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	positive regulation of kinase activity Inferred from genetic interaction Ref.6. Source: SGD positive regulation of phosphatidylinositol biosynthetic process Inferred from genetic interaction Ref.6. Source: SGD
Cellular_component	PAS complex Inferred from direct assay Ref.9. Source: SGD fungal-type vacuole membrane Inferred from direct assay Ref.1. Source: SGD integral to membrane Inferred from direct assay Ref.1. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1165	1165	Vacuolar segregation protein 7		PRO_0000065757
Regions
	Topological domain	1 – 919	919	Cytoplasmic Potential
	Transmembrane	920 – 940	21	Helical; Signal-anchor for type II membrane protein; Potential
	Topological domain	941 – 1165	225	Vacuolar Potential
	Compositional bias	277 – 412	136	Asn-rich
	Compositional bias	469 – 485	17	Gln-rich
	Compositional bias	1085 – 1119	35	Asp-rich
Amino acid modifications
	Modified residue	258	1	Phosphothreonine Ref.10
	Modified residue	443	1	Phosphoserine Ref.10
	Modified residue	494	1	Phosphoserine Ref.8
	Modified residue	579	1	Phosphoserine Ref.10
	Modified residue	602	1	Phosphoserine Ref.10
	Modified residue	607	1	Phosphoserine Ref.10
	Glycosylation	1020	1	N-linked (GlcNAc...) Potential
	Glycosylation	1099	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	P53950 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 0414978AA3066CE7	FASTA	1,165	128,140
	10 20 30 40 50 60 MTEEDRKLTV ETETVEAPVA NNLLLSNNSN VVAPNPSIPS ASTSTSPLHR EIVDDSVATA 70 80 90 100 110 120 NTTSNVVQHN LPTIDNNLMD SDATSHNQDH WHSDINRAGT SMSTSDIPTD LHLEHIGSVS 130 140 150 160 170 180 STNNNSNNAL INHNPLSSHL SNPSSSLRNK KSSLLVASNP AFASDVELSK KKPAVISNNM 190 200 210 220 230 240 PTSNIALYQT ARSANIHGPS STSASKAFRK ASAFSNNTAP STSNNIGSNT PPAPLLPLPS 250 260 270 280 290 300 LSQQNKPKII ERPTMHVTNS REILLGENLL DDTKAKNAPA NSTTHDNGPV ANDGLRIPNH 310 320 330 340 350 360 SNADDNENNN KMKKNKNINS GKNERNDDTS KICTTSTKTA PSTAPLGSTD NTQALTASVS 370 380 390 400 410 420 SSNADNHNNN KKKTSSNNNG NNSNSASNKT NADIKNSNAD LSASTSNNNA INDDSHESNS 430 440 450 460 470 480 EKPTKADFFA ARLATAVGEN EISDSEETFV YESAANSTKN LIFPDSSSQQ QQQQQQPPKQ 490 500 510 520 530 540 QQQQQNHGIT SKISAPLLNN NKKLLSRLKN SRHISTGAIL NNTIATISTN PNLNSNVMQN 550 560 570 580 590 600 NNNLMSGHNH LDELSSIKQE PPHQLQQQQP PMDVQSVDSY TSDNPDSNVI AKSPDKRSSL 610 620 630 640 650 660 VSLSKVSPHL LSSTSSNGNT ISCPNVATNS QELEPNNDIS TKKSLSNSTL RHSSANRNSN 670 680 690 700 710 720 YGDNKRPLRT TVSKIFDSNP NGAPLRRYSG VPDHVNLEDY IEQPHNYPTM QNSVKKDEFY 730 740 750 760 770 780 NSRNNKFPHG LNFYGDNNVI EEENNGDSSN VNRPQHTNLQ HEFIPEDNES DENDIHSMFY 790 800 810 820 830 840 YNHKNDLETK PLISDYGEDE DVDDYDRPNA TFNSYYGSAS NTHELPLHGR MPSRSNNDYY 850 860 870 880 890 900 DFMVGNNTGN NNQLNEYTPL RMKRGQRHLS RTNNSIMNGS IHMNGNDDVT HSNINNNDIV 910 920 930 940 950 960 GYSPHNFYSR KSPFVKVKNF LYLAFVISSL LMTGFILGFL LATNKELQDV DVVVMDNVIS 970 980 990 1000 1010 1020 SSDELIFDIT VSAFNPGFFS ISVSQVDLDI FAKSSYLKCD SNGDCTVMEQ ERKILQITTN 1030 1040 1050 1060 1070 1080 LSLVEESANN DISGGNIETV LLGTAKKLET PLKFQGGAFN RNYDVSVSSV KLLSPGSREA 1090 1100 1110 1120 1130 1140 KHENDDDDDD DGDDGDDENN TNERQYKSKP NARDDKEDDT KKWKLLIKHD YELIVRGSMK 1150 1160 YEVPFFNTQK STAIQKDSMV HPGKK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Vac7p, a novel vacuolar protein, is required for normal vacuole inheritance and morphology." Bonangelino C.J., Catlett N.L., Weisman L.S. Mol. Cell. Biol. 17:6847-6858(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, TOPOLOGY, SUBCELLULAR LOCATION.
[2]	"The sequence of a 44 420 bp fragment located on the left arm of chromosome XIV from Saccharomyces cerevisiae." Bergez P., Doignon F., Crouzet M. Yeast 11:967-974(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: S288c / FY1676.
[3]	Erratum Bergez P., Doignon F., Crouzet M. Yeast 12:297-297(1996) [PubMed] [Europe PMC] [Abstract]
[4]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications." Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J. , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J. Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[5]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[6]	"Regulation of Fab1 phosphatidylinositol 3-phosphate 5-kinase pathway by Vac7 protein and Fig4, a polyphosphoinositide phosphatase family member." Gary J.D., Sato T.K., Stefan C.J., Bonangelino C.J., Weisman L.S., Emr S.D. Mol. Biol. Cell 13:1238-1251(2002) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[7]	"The Vac14p-Fig4p complex acts independently of Vac7p and couples PI3,5P2 synthesis and turnover." Duex J.E., Tang F., Weisman L.S. J. Cell Biol. 172:693-704(2006) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[8]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-494, MASS SPECTROMETRY.
[9]	"VAC14 nucleates a protein complex essential for the acute interconversion of PI3P and PI(3,5)P(2) in yeast and mouse." Jin N., Chow C.Y., Liu L., Zolov S.N., Bronson R., Davisson M., Petersen J.L., Zhang Y., Park S., Duex J.E., Goldowitz D., Meisler M.H., Weisman L.S. EMBO J. 27:3221-3234(2008) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION IN THE PI(3,5)P2 REGULATORY COMPLEX, FUNCTION, SUBCELLULAR LOCATION.
[10]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-258; SER-443; SER-579; SER-602 AND SER-607, MASS SPECTROMETRY.
[11]	"Global analysis of the glycoproteome in Saccharomyces cerevisiae reveals new roles for protein glycosylation in eukaryotes." Kung L.A., Tao S.-C., Qian J., Smith M.G., Snyder M., Zhu H. Mol. Syst. Biol. 5:308-308(2009) [PubMed] [Europe PMC] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U12141 Genomic DNA. Translation: AAA99658.1. Z71330 Genomic DNA. Translation: CAA95925.1. BK006947 Genomic DNA. Translation: DAA10491.1.
PIR	S62982.
RefSeq	NP_014344.3. NM_001182893.3.
3D structure databases
ProteinModelPortal	P53950.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-4368N.
IntAct	P53950. 2 interactions.
MINT	MINT-531363.
STRING	4932.YNL054W.
Proteomic databases
PaxDb	P53950.
PRIDE	P53950.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YNL054W; YNL054W; YNL054W.
GeneID	855673.
KEGG	sce:YNL047C. sce:YNL054W.
Organism-specific databases
CYGD	YNL054w.
SGD	S000004999. VAC7.
Phylogenomic databases
eggNOG	NOG42444.
GeneTree	ENSGT00640000092546.
OMA	KANTENT.
OrthoDB	EOG4KPXKQ.
Enzyme and pathway databases
BioCyc	MetaCyc:MONOMER-15184.
Gene expression databases
Genevestigator	P53950.
GermOnline	YNL054W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR024260. Vac7. [Graphical view]
Pfam	PF12751. Vac7. 2 hits. [Graphical view]
ProtoNet	Search...
Other
NextBio	979957.

Entry information

Entry name

VAC7_YEAST

Accession

Primary (citable) accession number: P53950
Secondary accession number(s): D6W1C5

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	April 3, 2013
This is version 86 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program