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P53950

- VAC7_YEAST

UniProt

P53950 - VAC7_YEAST

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Protein

Vacuolar segregation protein 7

Gene

VAC7

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

The PI(3,5)P2 regulatory complex regulates both the synthesis and turnover of phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2). Positively regulates FAB1 kinase activity. Major activator of FAB1 during hyperosmotic shock and can elevate levels of PtdIns(3,5)P2 in the absence of VAC14 and FIG4. Directly involved in vacuolar membrane scission. Required for normal vacuole acidification, inheritance and morphology.4 Publications

GO - Biological processi

  1. positive regulation of kinase activity Source: SGD
  2. positive regulation of phosphatidylinositol biosynthetic process Source: SGD
Complete GO annotation...

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-15184.
YEAST:G3O-33086-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Vacuolar segregation protein 7
Gene namesi
Name:VAC7
Ordered Locus Names:YNL054W
ORF Names:N2467, YNL2467W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XIV

Organism-specific databases

CYGDiYNL054w.
SGDiS000004999. VAC7.

Subcellular locationi

Vacuole membrane 2 Publications; Single-pass type II membrane protein 2 Publications

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 919919CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei920 – 94021Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
BLAST
Topological domaini941 – 1165225VacuolarSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. fungal-type vacuole membrane Source: SGD
  2. integral component of membrane Source: SGD
  3. PAS complex Source: SGD
  4. vacuolar membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Vacuole

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 11651165Vacuolar segregation protein 7PRO_0000065757Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei164 – 1641Phosphoserine1 Publication
Glycosylationi1020 – 10201N-linked (GlcNAc...)Sequence Analysis
Glycosylationi1099 – 10991N-linked (GlcNAc...)Sequence Analysis

Post-translational modificationi

N-glycosylated.1 Publication

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

MaxQBiP53950.
PaxDbiP53950.
PRIDEiP53950.

Expressioni

Gene expression databases

GenevestigatoriP53950.

Interactioni

Subunit structurei

Component of the PI(3,5)P2 regulatory complex, composed of ATG18, FIG4, FAB1, VAC14 and VAC7. VAC14 nucleates the assembly of the complex and serves as a scaffold.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
VAC14Q067084EBI-28714,EBI-27189

Protein-protein interaction databases

BioGridi35770. 41 interactions.
DIPiDIP-4368N.
IntActiP53950. 4 interactions.
MINTiMINT-531363.
STRINGi4932.YNL054W.

Structurei

3D structure databases

ProteinModelPortaliP53950.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi277 – 412136Asn-richAdd
BLAST
Compositional biasi469 – 48517Gln-richAdd
BLAST
Compositional biasi1085 – 111935Asp-richAdd
BLAST

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG42444.
InParanoidiP53950.
OMAiKANTENT.
OrthoDBiEOG74N5R8.

Family and domain databases

InterProiIPR024260. Vac7.
[Graphical view]
PfamiPF12751. Vac7. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P53950-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTEEDRKLTV ETETVEAPVA NNLLLSNNSN VVAPNPSIPS ASTSTSPLHR
60 70 80 90 100
EIVDDSVATA NTTSNVVQHN LPTIDNNLMD SDATSHNQDH WHSDINRAGT
110 120 130 140 150
SMSTSDIPTD LHLEHIGSVS STNNNSNNAL INHNPLSSHL SNPSSSLRNK
160 170 180 190 200
KSSLLVASNP AFASDVELSK KKPAVISNNM PTSNIALYQT ARSANIHGPS
210 220 230 240 250
STSASKAFRK ASAFSNNTAP STSNNIGSNT PPAPLLPLPS LSQQNKPKII
260 270 280 290 300
ERPTMHVTNS REILLGENLL DDTKAKNAPA NSTTHDNGPV ANDGLRIPNH
310 320 330 340 350
SNADDNENNN KMKKNKNINS GKNERNDDTS KICTTSTKTA PSTAPLGSTD
360 370 380 390 400
NTQALTASVS SSNADNHNNN KKKTSSNNNG NNSNSASNKT NADIKNSNAD
410 420 430 440 450
LSASTSNNNA INDDSHESNS EKPTKADFFA ARLATAVGEN EISDSEETFV
460 470 480 490 500
YESAANSTKN LIFPDSSSQQ QQQQQQPPKQ QQQQQNHGIT SKISAPLLNN
510 520 530 540 550
NKKLLSRLKN SRHISTGAIL NNTIATISTN PNLNSNVMQN NNNLMSGHNH
560 570 580 590 600
LDELSSIKQE PPHQLQQQQP PMDVQSVDSY TSDNPDSNVI AKSPDKRSSL
610 620 630 640 650
VSLSKVSPHL LSSTSSNGNT ISCPNVATNS QELEPNNDIS TKKSLSNSTL
660 670 680 690 700
RHSSANRNSN YGDNKRPLRT TVSKIFDSNP NGAPLRRYSG VPDHVNLEDY
710 720 730 740 750
IEQPHNYPTM QNSVKKDEFY NSRNNKFPHG LNFYGDNNVI EEENNGDSSN
760 770 780 790 800
VNRPQHTNLQ HEFIPEDNES DENDIHSMFY YNHKNDLETK PLISDYGEDE
810 820 830 840 850
DVDDYDRPNA TFNSYYGSAS NTHELPLHGR MPSRSNNDYY DFMVGNNTGN
860 870 880 890 900
NNQLNEYTPL RMKRGQRHLS RTNNSIMNGS IHMNGNDDVT HSNINNNDIV
910 920 930 940 950
GYSPHNFYSR KSPFVKVKNF LYLAFVISSL LMTGFILGFL LATNKELQDV
960 970 980 990 1000
DVVVMDNVIS SSDELIFDIT VSAFNPGFFS ISVSQVDLDI FAKSSYLKCD
1010 1020 1030 1040 1050
SNGDCTVMEQ ERKILQITTN LSLVEESANN DISGGNIETV LLGTAKKLET
1060 1070 1080 1090 1100
PLKFQGGAFN RNYDVSVSSV KLLSPGSREA KHENDDDDDD DGDDGDDENN
1110 1120 1130 1140 1150
TNERQYKSKP NARDDKEDDT KKWKLLIKHD YELIVRGSMK YEVPFFNTQK
1160
STAIQKDSMV HPGKK
Length:1,165
Mass (Da):128,140
Last modified:October 1, 1996 - v1
Checksum:i0414978AA3066CE7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U12141 Genomic DNA. Translation: AAA99658.1.
Z71330 Genomic DNA. Translation: CAA95925.1.
BK006947 Genomic DNA. Translation: DAA10491.1.
PIRiS62982.
RefSeqiNP_014344.3. NM_001182893.3.

Genome annotation databases

EnsemblFungiiYNL054W; YNL054W; YNL054W.
GeneIDi855673.
KEGGisce:YNL054W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U12141 Genomic DNA. Translation: AAA99658.1 .
Z71330 Genomic DNA. Translation: CAA95925.1 .
BK006947 Genomic DNA. Translation: DAA10491.1 .
PIRi S62982.
RefSeqi NP_014344.3. NM_001182893.3.

3D structure databases

ProteinModelPortali P53950.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 35770. 41 interactions.
DIPi DIP-4368N.
IntActi P53950. 4 interactions.
MINTi MINT-531363.
STRINGi 4932.YNL054W.

Proteomic databases

MaxQBi P53950.
PaxDbi P53950.
PRIDEi P53950.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YNL054W ; YNL054W ; YNL054W .
GeneIDi 855673.
KEGGi sce:YNL054W.

Organism-specific databases

CYGDi YNL054w.
SGDi S000004999. VAC7.

Phylogenomic databases

eggNOGi NOG42444.
InParanoidi P53950.
OMAi KANTENT.
OrthoDBi EOG74N5R8.

Enzyme and pathway databases

BioCyci MetaCyc:MONOMER-15184.
YEAST:G3O-33086-MONOMER.

Miscellaneous databases

NextBioi 979957.

Gene expression databases

Genevestigatori P53950.

Family and domain databases

InterProi IPR024260. Vac7.
[Graphical view ]
Pfami PF12751. Vac7. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Vac7p, a novel vacuolar protein, is required for normal vacuole inheritance and morphology."
    Bonangelino C.J., Catlett N.L., Weisman L.S.
    Mol. Cell. Biol. 17:6847-6858(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, TOPOLOGY, SUBCELLULAR LOCATION.
  2. "The sequence of a 44 420 bp fragment located on the left arm of chromosome XIV from Saccharomyces cerevisiae."
    Bergez P., Doignon F., Crouzet M.
    Yeast 11:967-974(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: S288c / FY1676.
  3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications."
    Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J.
    , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.
    Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. "Regulation of Fab1 phosphatidylinositol 3-phosphate 5-kinase pathway by Vac7 protein and Fig4, a polyphosphoinositide phosphatase family member."
    Gary J.D., Sato T.K., Stefan C.J., Bonangelino C.J., Weisman L.S., Emr S.D.
    Mol. Biol. Cell 13:1238-1251(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  6. "The Vac14p-Fig4p complex acts independently of Vac7p and couples PI3,5P2 synthesis and turnover."
    Duex J.E., Tang F., Weisman L.S.
    J. Cell Biol. 172:693-704(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  7. "VAC14 nucleates a protein complex essential for the acute interconversion of PI3P and PI(3,5)P(2) in yeast and mouse."
    Jin N., Chow C.Y., Liu L., Zolov S.N., Bronson R., Davisson M., Petersen J.L., Zhang Y., Park S., Duex J.E., Goldowitz D., Meisler M.H., Weisman L.S.
    EMBO J. 27:3221-3234(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN THE PI(3,5)P2 REGULATORY COMPLEX, FUNCTION, SUBCELLULAR LOCATION.
  8. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  9. "Global analysis of the glycoproteome in Saccharomyces cerevisiae reveals new roles for protein glycosylation in eukaryotes."
    Kung L.A., Tao S.-C., Qian J., Smith M.G., Snyder M., Zhu H.
    Mol. Syst. Biol. 5:308-308(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS].
  10. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-164, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiVAC7_YEAST
AccessioniPrimary (citable) accession number: P53950
Secondary accession number(s): D6W1C5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: October 29, 2014
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  2. Yeast chromosome XIV
    Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

External Data

Dasty 3