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P53950

- VAC7_YEAST

UniProt

P53950 - VAC7_YEAST

Protein

Vacuolar segregation protein 7

Gene

VAC7

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 96 (01 Oct 2014)
      Sequence version 1 (01 Oct 1996)
      Previous versions | rss
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    Functioni

    The PI(3,5)P2 regulatory complex regulates both the synthesis and turnover of phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2). Positively regulates FAB1 kinase activity. Major activator of FAB1 during hyperosmotic shock and can elevate levels of PtdIns(3,5)P2 in the absence of VAC14 and FIG4. Directly involved in vacuolar membrane scission. Required for normal vacuole acidification, inheritance and morphology.4 Publications

    GO - Molecular functioni

    1. protein binding Source: IntAct

    GO - Biological processi

    1. positive regulation of kinase activity Source: SGD
    2. positive regulation of phosphatidylinositol biosynthetic process Source: SGD

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-15184.
    YEAST:G3O-33086-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Vacuolar segregation protein 7
    Gene namesi
    Name:VAC7
    Ordered Locus Names:YNL054W
    ORF Names:N2467, YNL2467W
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XIV

    Organism-specific databases

    CYGDiYNL054w.
    SGDiS000004999. VAC7.

    Subcellular locationi

    Vacuole membrane 2 Publications; Single-pass type II membrane protein 2 Publications

    GO - Cellular componenti

    1. fungal-type vacuole membrane Source: SGD
    2. integral component of membrane Source: SGD
    3. PAS complex Source: SGD
    4. vacuolar membrane Source: UniProtKB

    Keywords - Cellular componenti

    Membrane, Vacuole

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 11651165Vacuolar segregation protein 7PRO_0000065757Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei164 – 1641Phosphoserine1 Publication
    Glycosylationi1020 – 10201N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi1099 – 10991N-linked (GlcNAc...)Sequence Analysis

    Post-translational modificationi

    N-glycosylated.1 Publication

    Keywords - PTMi

    Glycoprotein, Phosphoprotein

    Proteomic databases

    MaxQBiP53950.
    PaxDbiP53950.
    PRIDEiP53950.

    Expressioni

    Gene expression databases

    GenevestigatoriP53950.

    Interactioni

    Subunit structurei

    Component of the PI(3,5)P2 regulatory complex, composed of ATG18, FIG4, FAB1, VAC14 and VAC7. VAC14 nucleates the assembly of the complex and serves as a scaffold.1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    VAC14Q067084EBI-28714,EBI-27189

    Protein-protein interaction databases

    BioGridi35770. 41 interactions.
    DIPiDIP-4368N.
    IntActiP53950. 4 interactions.
    MINTiMINT-531363.
    STRINGi4932.YNL054W.

    Structurei

    3D structure databases

    ProteinModelPortaliP53950.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 919919CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini941 – 1165225VacuolarSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei920 – 94021Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi277 – 412136Asn-richAdd
    BLAST
    Compositional biasi469 – 48517Gln-richAdd
    BLAST
    Compositional biasi1085 – 111935Asp-richAdd
    BLAST

    Keywords - Domaini

    Signal-anchor, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG42444.
    OMAiKANTENT.
    OrthoDBiEOG74N5R8.

    Family and domain databases

    InterProiIPR024260. Vac7.
    [Graphical view]
    PfamiPF12751. Vac7. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P53950-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTEEDRKLTV ETETVEAPVA NNLLLSNNSN VVAPNPSIPS ASTSTSPLHR     50
    EIVDDSVATA NTTSNVVQHN LPTIDNNLMD SDATSHNQDH WHSDINRAGT 100
    SMSTSDIPTD LHLEHIGSVS STNNNSNNAL INHNPLSSHL SNPSSSLRNK 150
    KSSLLVASNP AFASDVELSK KKPAVISNNM PTSNIALYQT ARSANIHGPS 200
    STSASKAFRK ASAFSNNTAP STSNNIGSNT PPAPLLPLPS LSQQNKPKII 250
    ERPTMHVTNS REILLGENLL DDTKAKNAPA NSTTHDNGPV ANDGLRIPNH 300
    SNADDNENNN KMKKNKNINS GKNERNDDTS KICTTSTKTA PSTAPLGSTD 350
    NTQALTASVS SSNADNHNNN KKKTSSNNNG NNSNSASNKT NADIKNSNAD 400
    LSASTSNNNA INDDSHESNS EKPTKADFFA ARLATAVGEN EISDSEETFV 450
    YESAANSTKN LIFPDSSSQQ QQQQQQPPKQ QQQQQNHGIT SKISAPLLNN 500
    NKKLLSRLKN SRHISTGAIL NNTIATISTN PNLNSNVMQN NNNLMSGHNH 550
    LDELSSIKQE PPHQLQQQQP PMDVQSVDSY TSDNPDSNVI AKSPDKRSSL 600
    VSLSKVSPHL LSSTSSNGNT ISCPNVATNS QELEPNNDIS TKKSLSNSTL 650
    RHSSANRNSN YGDNKRPLRT TVSKIFDSNP NGAPLRRYSG VPDHVNLEDY 700
    IEQPHNYPTM QNSVKKDEFY NSRNNKFPHG LNFYGDNNVI EEENNGDSSN 750
    VNRPQHTNLQ HEFIPEDNES DENDIHSMFY YNHKNDLETK PLISDYGEDE 800
    DVDDYDRPNA TFNSYYGSAS NTHELPLHGR MPSRSNNDYY DFMVGNNTGN 850
    NNQLNEYTPL RMKRGQRHLS RTNNSIMNGS IHMNGNDDVT HSNINNNDIV 900
    GYSPHNFYSR KSPFVKVKNF LYLAFVISSL LMTGFILGFL LATNKELQDV 950
    DVVVMDNVIS SSDELIFDIT VSAFNPGFFS ISVSQVDLDI FAKSSYLKCD 1000
    SNGDCTVMEQ ERKILQITTN LSLVEESANN DISGGNIETV LLGTAKKLET 1050
    PLKFQGGAFN RNYDVSVSSV KLLSPGSREA KHENDDDDDD DGDDGDDENN 1100
    TNERQYKSKP NARDDKEDDT KKWKLLIKHD YELIVRGSMK YEVPFFNTQK 1150
    STAIQKDSMV HPGKK 1165
    Length:1,165
    Mass (Da):128,140
    Last modified:October 1, 1996 - v1
    Checksum:i0414978AA3066CE7
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U12141 Genomic DNA. Translation: AAA99658.1.
    Z71330 Genomic DNA. Translation: CAA95925.1.
    BK006947 Genomic DNA. Translation: DAA10491.1.
    PIRiS62982.
    RefSeqiNP_014344.3. NM_001182893.3.

    Genome annotation databases

    EnsemblFungiiYNL054W; YNL054W; YNL054W.
    GeneIDi855673.
    KEGGisce:YNL054W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U12141 Genomic DNA. Translation: AAA99658.1 .
    Z71330 Genomic DNA. Translation: CAA95925.1 .
    BK006947 Genomic DNA. Translation: DAA10491.1 .
    PIRi S62982.
    RefSeqi NP_014344.3. NM_001182893.3.

    3D structure databases

    ProteinModelPortali P53950.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 35770. 41 interactions.
    DIPi DIP-4368N.
    IntActi P53950. 4 interactions.
    MINTi MINT-531363.
    STRINGi 4932.YNL054W.

    Proteomic databases

    MaxQBi P53950.
    PaxDbi P53950.
    PRIDEi P53950.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YNL054W ; YNL054W ; YNL054W .
    GeneIDi 855673.
    KEGGi sce:YNL054W.

    Organism-specific databases

    CYGDi YNL054w.
    SGDi S000004999. VAC7.

    Phylogenomic databases

    eggNOGi NOG42444.
    OMAi KANTENT.
    OrthoDBi EOG74N5R8.

    Enzyme and pathway databases

    BioCyci MetaCyc:MONOMER-15184.
    YEAST:G3O-33086-MONOMER.

    Miscellaneous databases

    NextBioi 979957.

    Gene expression databases

    Genevestigatori P53950.

    Family and domain databases

    InterProi IPR024260. Vac7.
    [Graphical view ]
    Pfami PF12751. Vac7. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Vac7p, a novel vacuolar protein, is required for normal vacuole inheritance and morphology."
      Bonangelino C.J., Catlett N.L., Weisman L.S.
      Mol. Cell. Biol. 17:6847-6858(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, TOPOLOGY, SUBCELLULAR LOCATION.
    2. "The sequence of a 44 420 bp fragment located on the left arm of chromosome XIV from Saccharomyces cerevisiae."
      Bergez P., Doignon F., Crouzet M.
      Yeast 11:967-974(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: S288c / FY1676.
    3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications."
      Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J.
      , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.
      Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    5. "Regulation of Fab1 phosphatidylinositol 3-phosphate 5-kinase pathway by Vac7 protein and Fig4, a polyphosphoinositide phosphatase family member."
      Gary J.D., Sato T.K., Stefan C.J., Bonangelino C.J., Weisman L.S., Emr S.D.
      Mol. Biol. Cell 13:1238-1251(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    6. "The Vac14p-Fig4p complex acts independently of Vac7p and couples PI3,5P2 synthesis and turnover."
      Duex J.E., Tang F., Weisman L.S.
      J. Cell Biol. 172:693-704(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    7. "VAC14 nucleates a protein complex essential for the acute interconversion of PI3P and PI(3,5)P(2) in yeast and mouse."
      Jin N., Chow C.Y., Liu L., Zolov S.N., Bronson R., Davisson M., Petersen J.L., Zhang Y., Park S., Duex J.E., Goldowitz D., Meisler M.H., Weisman L.S.
      EMBO J. 27:3221-3234(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION IN THE PI(3,5)P2 REGULATORY COMPLEX, FUNCTION, SUBCELLULAR LOCATION.
    8. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    9. "Global analysis of the glycoproteome in Saccharomyces cerevisiae reveals new roles for protein glycosylation in eukaryotes."
      Kung L.A., Tao S.-C., Qian J., Smith M.G., Snyder M., Zhu H.
      Mol. Syst. Biol. 5:308-308(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS].
    10. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-164, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiVAC7_YEAST
    AccessioniPrimary (citable) accession number: P53950
    Secondary accession number(s): D6W1C5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 96 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    2. Yeast chromosome XIV
      Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

    External Data

    Dasty 3