P53944 (MTQ1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Mitochondrial N(5)-glutamine methyltransferase MTQ1
EC=2.1.1.-

Gene names

Name:	MTQ1
Ordered Locus Names:	YNL063W
ORF Names:	N2420, YNL2420W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	314 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Methylates MRF1 on 'Gln-287' using S-adenosyl L-methionine as methyl donor. Ref.8
Subcellular location	Mitochondrion Ref.6 Ref.8.
Miscellaneous	Present with 13200 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the protein N5-glutamine methyltransferase family.

Ontologies

Keywords
Cellular component	Mitochondrion
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	translational readthrough Inferred from genetic interaction Ref.8. Source: SGD
Cellular_component	mitochondrion Inferred from direct assay Ref.6. Source: SGD
Molecular_function	S-adenosylmethionine-dependent methyltransferase activity Inferred from direct assay Ref.8. Source: SGD protein methyltransferase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
MRF1	P30775	2	EBI-28737,EBI-14964

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 314	314	Mitochondrial N(5)-glutamine methyltransferase MTQ1		PRO_0000203448
Regions
	Region	118 – 122	5	S-adenosyl-L-methionine binding By similarity
	Region	188 – 191	4	Substrate binding By similarity
Sites
	Binding site	141	1	S-adenosyl-L-methionine By similarity
	Binding site	188	1	S-adenosyl-L-methionine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P53944 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 2281F44B511EAA65	FASTA	314	35,871
	10 20 30 40 50 60 MPRISTSLIR KASRIRPGLH LLLPECRTLE QAKLEYKWLT EELPPDKSIR WACLQRYKHV 70 80 90 100 110 120 PLQYILRSQP FGALDIVCKP GVLIPRWETE EWVMAIIRAL NNSMLSRHTI PLHICDTFTG 130 140 150 160 170 180 TGCIALALSH GIANCTFTAI DVSTRAIKLV KENMLKNKVS GGKLVQHNIL SSKASDEYPS 190 200 210 220 230 240 HIDILTGNPP YIRKRDFNRD VKTSVKLFEP RLALVGELEC YINLVNYWLP KTDSFFYEIG 250 260 270 280 290 300 DVEQFNYVER RIKEDSYLSR IWSIGLKYDS NGKARVVYGF KATPKGRILH QIFASFGTIR 310 HLATALSGHK ANCN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The sequence of a 44 420 bp fragment located on the left arm of chromosome XIV from Saccharomyces cerevisiae." Bergez P., Doignon F., Crouzet M. Yeast 11:967-974(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: S288c / FY1676.
[2]	Erratum Bergez P., Doignon F., Crouzet M. Yeast 12:297-297(1996) [PubMed] [Europe PMC] [Abstract]
[3]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications." Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J. , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J. Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[5]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[6]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[7]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[8]	"The yeast translation release factors Mrf1p and Sup45p (eRF1) are methylated, respectively, by the methyltransferases Mtq1p and Mtq2p." Polevoda B., Span L., Sherman F. J. Biol. Chem. 281:2562-2571(2006) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U12141 Genomic DNA. Translation: AAA99648.1. Z71339 Genomic DNA. Translation: CAA95936.1. AY692585 Genomic DNA. Translation: AAT92604.1. BK006947 Genomic DNA. Translation: DAA10483.1.
PIR	S58715.
RefSeq	NP_014336.1. NM_001182901.1.
3D structure databases
ProteinModelPortal	P53944.
SMR	P53944. Positions 55-275.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-6545N.
IntAct	P53944. 4 interactions.
MINT	MINT-677281.
STRING	4932.YNL063W.
Proteomic databases
PaxDb	P53944.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YNL063W; YNL063W; YNL063W.
GeneID	855662.
KEGG	sce:YNL063W.
Organism-specific databases
CYGD	YNL063w.
SGD	S000005007. MTQ1.
Phylogenomic databases
eggNOG	COG2890.
GeneTree	ENSGT00390000014125.
HOGENOM	HOG000248841.
OMA	SFVYEVG.
OrthoDB	EOG4X0R2M.
Gene expression databases
Genevestigator	P53944.
GermOnline	YNL063W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR004556. Modification_methylase_HemK. IPR007848. Small_mtfrase_dom. [Graphical view]
Pfam	PF05175. MTS. 1 hit. [Graphical view]
TIGRFAMs	TIGR00536. hemK_fam. 1 hit.
PROSITE	PS00092. N6_MTASE. False negative. [Graphical view]
ProtoNet	Search...
Other
NextBio	979929.

Entry information

Entry name

MTQ1_YEAST

Accession

Primary (citable) accession number: P53944
Secondary accession number(s): D6W1B7

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	May 1, 2013
This is version 100 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program